Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| detection of tetracycline resistance genes in bacteria isolated from fish farms using polymerase chain reaction. | five common tetracycline resistance genes tet(a), tet(b), tet(m), tet(o) and tet(s) were studied by polymerase chain reaction in 100 bacteria isolated from iranian fish farms. in the antibiogram test most of the bacteria were either intermediately or completely resistant to tetracycline. nine isolates out of 46 aeromonas spp. contained either tet(a/m/s) resistant genes as follows: tet(a) in a. veronii/sobria (n = 1), a. media (n = 2), a. aquariorum (n = 1), and a. veronii (n = 3); tet(m) in one ... | 2014 | 25610578 |
| investigation of antibacterial, acid and bile tolerance properties of lactobacilli isolated from koozeh cheese. | lactobacillus strains are a major part of the probiotics, microflora of the intestine and of fermented dairy products, and are found in a variety of environments. the aim of this study was to find out the ability of bile and acid tolerance and antibacterial properties of the twenty eight isolates of three group lactobacilli namely lactobacillus plantarum, lactobacillus casei and lactobacillus delbruki. for this purpose twenty eight different lactobacillus strains that isolated from koozeh cheese ... | 2012 | 25610566 |
| guillain-barré syndrome during active brucellosis. | it has been reported that approximately two thirds of patients diagnosed with guillain-barré syndrome (gbs) have had a prodromal illness within a four-week period preceding the onset of gbs. this prodromal illness is most commonly an upper respiratory tract illness or, secondly, gastroenteritis. however, specific infectious agents, such as mycoplasma, cytomegalovirus, ebstein-barr virus, vaccinia, variola, campylobacter, varicella-zoster, measles, mumps, hepatitis a and b viruses, rubella, influ ... | 2010 | 25610149 |
| illness in breastfeeding infants relates to concentration of lactoferrin and secretory immunoglobulin a in mother's milk. | this study aims to better understand the relationship between immune compounds in human milk and infant health. we hypothesized that the concentration of immune compounds in milk would relate to infant illness symptoms according to two possible theoretical paradigms. in the 'protective' paradigm, high concentrations of immune compounds prevent infant illness. the converse, the 'responsive' framework, posits that concentrations of immune compounds are elevated in response to infection. | 2015 | 25608691 |
| mouse genome engineering via crispr-cas9 for study of immune function. | clustered regularly interspaced palindromic repeats (crispr)-associated (cas9) technology has proven a formidable addition to our armory of approaches for genomic editing. derived from pathways in archaea and bacteria that mediate the resistance to exogenous genomic material, the crispr-cas9 system utilizes a short single guide rna (sgrna) to direct the endonuclease cas9 to virtually anywhere in the genome. upon targeting, cas9 generates dna double-strand breaks (dsbs) and facilitates the repair ... | 2015 | 25607456 |
| a man with febrile illness and difficulty in walking. | 2008 | 25606113 | |
| type iv pilus glycosylation mediates resistance of pseudomonas aeruginosa to opsonic activities of the pulmonary surfactant protein a. | pseudomonas aeruginosa is a major bacterial pathogen commonly associated with chronic lung infections in cystic fibrosis (cf). previously, we have demonstrated that the type iv pilus (tfp) of p. aeruginosa mediates resistance to antibacterial effects of pulmonary surfactant protein a (sp-a). interestingly, p. aeruginosa strains with group i pilins are o-glycosylated through the tfpo glycosyltransferase with a single subunit of o-antigen (o-ag). importantly, tfpo-mediated o-glycosylation is impor ... | 2015 | 25605768 |
| sialylation of helicobacter bizzozeronii lipopolysaccharides modulates toll-like receptor (tlr) 2 mediated response. | sialic acid in lipopolysaccharides (lps) of mucosal pathogens is known to be an important virulence factor. few strains of helicobacter pylori express sialyl-lewis-x and we have reported that human and canine helicobacter bizzozeronii strains express sialyl-lactoseamine in their lps. however, the role of sialyation of helicobacter lps in the interaction with the host cells is still unknown. in this study h. bizzozeronii lps is shown to activate the tlr2 in a dose and strain dependent manner in t ... | 2015 | 25603825 |
| transcriptional regulation of bacterial virulence gene expression by molecular oxygen and nitric oxide. | molecular oxygen (o2) and nitric oxide (no) are diatomic gases that play major roles in infection. the host innate immune system generates reactive oxygen species and no as bacteriocidal agents and both require o2 for their production. furthermore, the ability to adapt to changes in o2 availability is crucial for many bacterial pathogens, as many niches within a host are hypoxic. pathogenic bacteria have evolved transcriptional regulatory systems that perceive these gases and respond by reprogra ... | 2014 | 25603427 |
| campylobacter jejuni acquire new host-derived crispr spacers when in association with bacteriophages harboring a crispr-like cas4 protein. | campylobacter jejuni is a worldwide cause of human diarrhoeal disease. clustered repetitively interspaced palindromic repeats (crisprs) and associated proteins allow bacteria and archaea to evade bacteriophage and plasmid infection. type ii crispr systems are found in association with combinations of genes encoding the crispr-associated cas1, cas2, cas4 or csn2, and cas9 proteins. c. jejuni possesses a minimal subtype ii-c crispr system containing cas1, cas2, and cas9 genes whilst cas4 is notabl ... | 2014 | 25601859 |
| campylobacter jejuni acquire new host-derived crispr spacers when in association with bacteriophages harboring a crispr-like cas4 protein. | campylobacter jejuni is a worldwide cause of human diarrhoeal disease. clustered repetitively interspaced palindromic repeats (crisprs) and associated proteins allow bacteria and archaea to evade bacteriophage and plasmid infection. type ii crispr systems are found in association with combinations of genes encoding the crispr-associated cas1, cas2, cas4 or csn2, and cas9 proteins. c. jejuni possesses a minimal subtype ii-c crispr system containing cas1, cas2, and cas9 genes whilst cas4 is notabl ... | 2014 | 25601859 |
| how well does physician selection of microbiologic tests identify clostridium difficile and other pathogens in paediatric diarrhoea? insights using multiplex pcr-based detection. | the objective of this study was to compare the aetiologic yield of standard-of-care microbiologic testing ordered by physicians with that of a multiplex pcr platform. stool specimens obtained from children and young adults with gastrointestinal illness were evaluated by standard laboratory methods and a developmental version of the filmarray gastrointestinal (gi) diagnostic system (filmarray gi panel), a rapid multiplex pcr platform that detects 23 bacterial, viral and protozoal agents. results ... | 2014 | 25599941 |
| how well does physician selection of microbiologic tests identify clostridium difficile and other pathogens in paediatric diarrhoea? insights using multiplex pcr-based detection. | the objective of this study was to compare the aetiologic yield of standard-of-care microbiologic testing ordered by physicians with that of a multiplex pcr platform. stool specimens obtained from children and young adults with gastrointestinal illness were evaluated by standard laboratory methods and a developmental version of the filmarray gastrointestinal (gi) diagnostic system (filmarray gi panel), a rapid multiplex pcr platform that detects 23 bacterial, viral and protozoal agents. results ... | 2014 | 25599941 |
| identification and characterization of a putative chemotaxis protein, chey, from the oral pathogen campylobacter rectus. | campylobacter rectus is an understudied oral bacterium that contributes to periodontitis. processes that contribute to the disease-causing capabilities of pathogens, such as chemotaxis, are largely unknown in c. rectus. the aim of this study was to better understand c. rectus chemotaxis, by examining the c. rectus genome for the presence of a chey gene. chey proteins play a part in chemotaxis by acting as two-component response regulators. significantly, chey proteins from several pathogens, inc ... | 2014 | 25598863 |
| chemical composition and antimicrobial activity of essential oils of juniperus excelsa bieb. (cupressaceae) grown in r. macedonia. | there are no information of the yield, chemical composition and antimicrobial activity of essential oils of berries (eob) or leaves (eol) of juniperus excelsa bieb. (cupressaceae) growing wild in r. macedonia. | 2015 | 25598638 |
| antibiotic susceptibility and prevalence of foodborne pathogens in poultry meat in romania. | the occurrence of pathogenic strains in poultry meat is of growing concern in romania. another problem found on a global level is the continuous increase of antimicrobial resistance in bacteria isolated from food. this study aimed to evaluate the prevalence of pathogenic bacteria in poultry carcasses obtained in romania in 2012-2013 and to reveal the most prevalent patterns of antimicrobial resistance in the isolated strains. | 2015 | 25596569 |
| characterizing the genetic basis of bacterial phenotypes using genome-wide association studies: a new direction for bacteriology. | genome-wide association studies (gwass) have become an increasingly important approach for eukaryotic geneticists, facilitating the identification of hundreds of genetic polymorphisms that are responsible for inherited diseases. despite the relative simplicity of bacterial genomes, the application of gwass to identify polymorphisms responsible for important bacterial phenotypes has only recently been made possible through advances in genome sequencing technologies. bacterial gwass are now about ... | 2014 | 25593593 |
| reprogramming of yersinia from virulent to persistent mode revealed by complex in vivo rna-seq analysis. | we recently found that yersinia pseudotuberculosis can be used as a model of persistent bacterial infections. we performed in vivo rna-seq of bacteria in small cecal tissue biopsies at early and persistent stages of infection to determine strategies associated with persistence. comprehensive analysis of mixed rna populations from infected tissues revealed that y. pseudotuberculosis undergoes transcriptional reprogramming with drastic down-regulation of t3ss virulence genes during persistence whe ... | 2015 | 25590628 |
| bacterial flagella: twist and stick, or dodge across the kingdoms. | the flagellum organelle is an intricate multiprotein assembly best known for its rotational propulsion of bacteria. however, recent studies have expanded our knowledge of other functions in pathogenic contexts, particularly adherence and immune modulation, e.g., for salmonella enterica, campylobacter jejuni, pseudomonas aeruginosa, and escherichia coli. flagella-mediated adherence is important in host colonisation for several plant and animal pathogens, but the specific interactions that promote ... | 2015 | 25590430 |
| overview and historical perspectives. | in this overview, we describe the history of shiga toxin (stx)-producing escherichia coli (stec) in two phases. in phase one, between 1977 and 2011, we learned that e. coli could produce shiga toxin and cause both hemorrhagic colitis and the hemolytic-uremic syndrome in humans and that the prototype stec-e. coli o157:h7-adheres to and effaces intestinal epithelial cells by a mechanism similar to that of enteropathogenic e. coli. we also recognized that the genes for stx are typically encoded on ... | 2014 | 25590020 |
| detection of mycobacteria, mycobacterium avium subspecies, and mycobacterium tuberculosis complex by a novel tetraplex real-time pcr assay. | mycobacterium tuberculosis complex, mycobacterium avium, and many other nontuberculous mycobacteria are worldwide distributed microorganisms of major medical and veterinary importance. considering the growing epidemiologic significance of wildlife-livestock-human interrelation, developing rapid detection tools of high specificity and sensitivity is vital to assess their presence and accelerate the process of diagnosing mycobacteriosis. here we describe the development and evaluation of a novel t ... | 2015 | 25588660 |
| primary isolation strain determines both phage type and receptors recognised by campylobacter jejuni bacteriophages. | in this study we isolated novel bacteriophages, infecting the zoonotic bacterium campylobacter jejuni. these phages may be used in phage therapy of c. jejuni colonized poultry to prevent spreading of the bacteria to meat products causing disease in humans. many c. jejuni phages have been isolated using nctc12662 as the indicator strain, which may have biased the selection of phages. a large group of c. jejuni phages rely on the highly diverse capsular polysaccharide (cps) for infection and recen ... | 2015 | 25585385 |
| effect of human isolated probiotic bacteria on preventing campylobacter jejuni colonization of poultry. | this study was performed in order to determine whether human isolated probiotic bacteria can be effective in reducing campylobacter jejuni infection of chicken intestinal cells, in vitro, and in decreasing its colonization abilities within the chicken gut. our results show that the probiotic strains lactobacillus paracasei j. r, l. rhamnosus 15b, l. lactis y, and l. lactis foa had a significant effect on c. jejuni invasion of chicken primary cells, with the strongest inhibitory effect detected w ... | 2015 | 25585278 |
| moraxella catarrhalis acrab-oprm efflux pump contributes to antimicrobial resistance and is enhanced during cold shock response. | moraxella catarrhalis is a common pathogen of the human respiratory tract. multidrug efflux pumps play a major role in antibiotic resistance and virulence in many gram-negative organisms. in the present study, the role of the acrab-oprm efflux pump in antibiotic resistance was investigated by constructing mutants that lack the acra, acrb, and oprm genes in m. catarrhalis strain o35e. we observed a moderate (1.5-fold) decrease in the mics of amoxicillin and cefotaxime and a marked (4.7-fold) decr ... | 2015 | 25583725 |
| rapid identification and classification of campylobacter spp. using laser optical scattering technology. | campylobacter jejuni and campylobacter coli are the two important species responsible for most of the campylobacter infections in humans. reliable isolation and detection of campylobacter spp. from food samples are challenging due to the interferences from complex food substances and the fastidious growth requirements of this organism. in this study, a novel biosensor-based detection called bardot (bacterial rapid detection using optical scattering technology) was developed for high-throughput s ... | 2015 | 25583335 |
| expression of the functional recombinant human glycosyltransferase galnact2 in escherichia coli. | recombinant protein-based therapeutics have become indispensable for the treatment of many diseases. they are produced using well-established expression systems based on bacteria, yeast, insect and mammalian cells. the majority of therapeutic proteins are glycoproteins and therefore the post-translational attachment of sugar residues is required. the development of an engineered escherichia coli-based expression system for production of human glycoproteins could potentially lead to increased yie ... | 2015 | 25582753 |
| lack of a type-2 glycosyltransferase in the fish pathogen flavobacterium psychrophilum determines pleiotropic changes and loss of virulence. | flavobacterium psychrophilum is an important fish pathogen, responsible for cold water disease, with a significant economic impact on salmonid farms worldwide. in spite of this, little is known about the bacterial physiology and pathogenesis mechanisms, maybe because it is difficult to manipulate, being considered a fastidious microorganism. mutants obtained using a tn4351 transposon were screened in order to identify those with alteration in colony morphology, colony spreading and extracellular ... | 2015 | 25582708 |
| the campylobacter jejuni cprrs two-component regulatory system regulates aspects of the cell envelope. | campylobacter jejuni is a leading cause of food-borne gastroenteritis in humans. it lives commensally in the gastrointestinal tract of animals, and tolerates variable conditions during transit/colonization of susceptible hosts. the c. jejuni cprrs two-component system contains an essential response regulator (cprr), and deletion of the cprs sensor kinase enhances biofilms. we sought to identify cprrs-regulated genes and better understand how the system affects survival. expression from the cprr ... | 2015 | 25582441 |
| microbiota-mediated inflammation and antimicrobial defense in the intestine. | the diverse microbial populations constituting the intestinal microbiota promote immune development and differentiation, but because of their complex metabolic requirements and the consequent difficulty culturing them, they remained, until recently, largely uncharacterized and mysterious. in the last decade, deep nucleic acid sequencing platforms, new computational and bioinformatics tools, and full-genome characterization of several hundred commensal bacterial species facilitated studies of the ... | 2015 | 25581310 |
| prevalence, antibiogram, and cdt genes of toxigenic campylobacter jejuni in salad style vegetables (ulam) at farms and retail outlets in terengganu. | the present study was conducted to investigate the prevalence and antibiotic resistance among campylobacter jejuni in ulam at farms and retail outlets located in kuala terengganu, malaysia. a total of 526 samples (ulam, soil, and fertilizer) were investigated for the presence of c. jejuni and the gene for cytolethal distending toxin (cdt) by using a multiplex pcr method. antibiotic susceptibility to 10 types of antibiotics was determined using the disk diffusion method for 33 c. jejuni isolates. ... | 2015 | 25581179 |
| molecular detection identified a type six secretion system in campylobacter jejuni from various sources but not from human cases. | to determine the presence of the t6ss in campylobacter jejuni from diverse sources. | 2015 | 25580664 |
| bacteria repelling poly(methylmethacrylate-co-dimethylacrylamide) coatings for biomedical devices†electronic supplementary information (esi) available: polymer microarray screening, including analysis of bacterial adhesion by fluorescence microscopy and sem, and chemical composition of bacteria repelling polymers identified in the screen; polymer synthesis and characterisation; preparation of catheter pieces and solvent studies, and details for confocal imaging/analysis. see doi: 10.1039/c4tb01129eclick here for additional data file. | nosocomial infections due to bacteria have serious implications on the health and recovery of patients in a variety of medical scenarios. since bacterial contamination on medical devices contributes to the majority of nosocomical infections, there is a need for redesigning the surfaces of medical devices, such as catheters and tracheal tubes, to resist the binding of bacteria. in this work, polyurethanes and polyacrylates/acrylamides, which resist binding by the major bacterial pathogens underpi ... | 2014 | 25580245 |
| storage, transport, release: heme versatility in nitrite reductase electron transfer studied by molecular dynamics simulations. | using molecular dynamics simulations of the thermodynamic integration type, we study the energetics and kinetics of electron transfer through the nitrite reductase enzyme of sulfurospirillum deleyianum, wolinella succinogenes and campylobacter jejuni. in all of these five-heme proteins, the storage of an even number of electrons within a monomeric chain is thermodynamically favoured. kinetically, two of these electrons are usually transferred almost simultaneously towards the active site. althou ... | 2015 | 25579411 |
| new role for the ankyrin repeat revealed by a study of the n-formyltransferase from providencia alcalifaciens. | n-formylated sugars such as 3,6-dideoxy-3-formamido-d-glucose (qui3nfo) have been observed on the lipopolysaccharides of various pathogenic bacteria, including providencia alcalifaciens, a known cause of gastroenteritis. these unusual carbohydrates are synthesized in vivo as dtdp-linked sugars. the biosynthetic pathway for the production of dtdp-qui3nfo requires five enzymes with the last step catalyzed by an n-formyltransferase that utilizes n(10)-tetrahydrofolate as a cofactor. here we describ ... | 2015 | 25574689 |
| in silico detection of virulence gene homologues in the human pathogen sphingomonas spp. | there is an ongoing debate about the clinical significance of sphingomonas paucimobilis as a virulent bacterial pathogen. in the present study, we investigated the presence of different virulence factors and genes in sphingomonas bacteria. we utilized phylogenetic, comparative genomics and bioinformatics analysis to investigate the potentiality of sphingomonas bacteria as virulent pathogenic bacteria. the 16s ribosomal rna gene (16s rdna) phylogenetic tree showed that the closest bacterial taxon ... | 2014 | 25574122 |
| giardia duodenalis-induced alterations of commensal bacteria kill caenorhabditis elegans: a new model to study microbial-microbial interactions in the gut. | giardia duodenalis is the most common cause of parasitic diarrhea worldwide and a well-established risk factor for postinfectious irritable bowel syndrome. we hypothesized that giardia-induced disruptions in host-microbiota interactions may play a role in the pathogenesis of giardiasis and in postgiardiasis disease. functional changes induced by giardia in commensal bacteria and the resulting effects on caenorhabditis elegans were determined. although giardia or bacteria alone did not affect wor ... | 2015 | 25573177 |
| [enumeration and antimicrobial resistance of campylobacter species from retail chicken carcasses]. | to determine campylobacter contamination level and antimicrobial resistance patterns from chicken carcasses in supermarkets and farmer's markets of 9 districts in beijing. | 2014 | 25573127 |
| a novel cofactor-binding mode in bacterial imp dehydrogenases explains inhibitor selectivity. | the steadily rising frequency of emerging diseases and antibiotic resistance creates an urgent need for new drugs and targets. inosine 5'-monophosphate dehydrogenase (imp dehydrogenase or impdh) is a promising target for the development of new antimicrobial agents. impdh catalyzes the oxidation of imp to xmp with the concomitant reduction of nad(+), which is the pivotal step in the biosynthesis of guanine nucleotides. potent inhibitors of bacterial impdhs have been identified that bind in a stru ... | 2015 | 25572472 |
| n-acetylcysteine and the human serum components that inhibit bacterial invasion of gingival epithelial cells prevent experimental periodontitis in mice. | we previously reported that human serum significantly reduces the invasion of various oral bacterial species into gingival epithelial cells in vitro. the aims of the present study were to characterize the serum component(s) responsible for the inhibition of bacterial invasion of epithelial cells and to examine their effect on periodontitis induced in mice. | 2014 | 25568806 |
| current and past strategies for bacterial culture in clinical microbiology. | a pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. the first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. the rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. th ... | 2015 | 25567228 |
| laboratory diagnosis of bacterial gastroenteritis. | bacterial gastroenteritis is a disease that is pervasive in both the developing and developed worlds. while for the most part bacterial gastroenteritis is self-limiting, identification of an etiological agent by bacterial stool culture is required for the management of patients with severe or prolonged diarrhea, symptoms consistent with invasive disease, or a history that may predict a complicated course of disease. importantly, characterization of bacterial enteropathogens from stool cultures i ... | 2015 | 25567220 |
| clinical importance and epidemiology of quinolone resistance. | the quinolone class of antimicrobial agents is one of most widely used classes of antimicrobial agents in outpatient and inpatient treatment. however, quinolone resistance in gram-positive and gram-negative bacteria has emerged and increased globally. this resistance limits the usefulness of quinolones in clinical practice. the review summarizes mechanisms of quinolone resistance and its epidemiology and implications in the most common clinical settings, urinary tract infections, respiratory tra ... | 2014 | 25566402 |
| is dosing of therapeutic immunoglobulins optimal? a review of a three-decade long debate in europe. | the consumption of immunoglobulins (ig) is increasing due to better recognition of antibody deficiencies, an aging population, and new indications. this review aims to examine the various dosing regimens and research developments in the established and in some of the relevant off-label indications in europe. the background to the current regulatory settings in europe is provided as a backdrop for the latest developments in primary and secondary immunodeficiencies and in immunomodulatory indicati ... | 2014 | 25566244 |
| the dam replacing gene product enhances neisseria gonorrhoeae fa1090 viability and biofilm formation. | many neisseriaceae do not exhibit dam methyltransferase activity and, instead of the dam gene, possess drg (dam replacing gene) inserted in the leus/dam locus. the drg locus in neisseria gonorrhoeae fa1090 has a lower gc-pairs content (40.5%) compared to the whole genome of n. gonorrhoeae fa1090 (52%). the gonococcal drg gene encodes a dna endonuclease drg, with gmeatc specificity. disruption of drg or insertion of the dam gene in gonococcal genome changes the level of expression of genes as sho ... | 2014 | 25566225 |
| vibrio parahaemolyticus: a review on the pathogenesis, prevalence, and advance molecular identification techniques. | vibrio parahaemolyticus is a gram-negative halophilic bacterium that is found in estuarine, marine and coastal environments. v. parahaemolyticus is the leading causal agent of human acute gastroenteritis following the consumption of raw, undercooked, or mishandled marine products. in rare cases, v. parahaemolyticus causes wound infection, ear infection or septicaemia in individuals with pre-existing medical conditions. v. parahaemolyticus has two hemolysins virulence factors that are thermostabl ... | 2014 | 25566219 |
| 5-ht7 receptor signaling: improved therapeutic strategy in gut disorders. | serotonin (5-hydroxytryptamine; 5-ht) is most commonly known for its role as a neurotransmitter in the central nervous system (cns). however, the majority of the body's 5-ht is produced in the gut by enterochromaffin (ec) cells. alterations in 5-ht signaling have been associated with various gut disorders including inflammatory bowel disease (ibd), irritable bowel syndrome (ibs) and enteric infections. recently, our studies have identified a key role for 5-ht in the pathogenesis of experimental ... | 2014 | 25565996 |
| cronobacter sakazakii: stress survival and virulence potential in an opportunistic foodborne pathogen. | a characteristic feature of the opportunistic foodborne pathogen cronobacter sakazakii is its ability to survive in extremely arid environments, such as powdered infant formula, making it a dangerous opportunistic pathogen of individuals of all age groups, especially infants and neonates. herein, we provide a brief overview of the pathogen; clinical manifestations, environmental reservoirs and our current understanding of stress response mechanisms and virulence factors which allow it to cause d ... | 2014 | 25562731 |
| cronobacter sakazakii: stress survival and virulence potential in an opportunistic foodborne pathogen. | a characteristic feature of the opportunistic foodborne pathogen cronobacter sakazakii is its ability to survive in extremely arid environments, such as powdered infant formula, making it a dangerous opportunistic pathogen of individuals of all age groups, especially infants and neonates. herein, we provide a brief overview of the pathogen; clinical manifestations, environmental reservoirs and our current understanding of stress response mechanisms and virulence factors which allow it to cause d ... | 2014 | 25562731 |
| colonic mucosa-associated lymphoid tissue lymphoma identified by chromoendoscopy. | colonic mucosa-associated lymphoid tissue (malt) lymphomas are a rare occurrence and the definitive treatment has not been established. solitary or multiple, elevated or polypoid lesions are the usual appearances of malt lymphoma in the large intestine and sometimes the surface may reveal abnormal vascularity. herein, we report a case of malt lymphoma and review the relevant literature. upon colonoscopy, a suspected pathologic lesion was observed in the proximal transverse colon. the lesion coul ... | 2014 | 25561821 |
| dismicrobism in inflammatory bowel disease and colorectal cancer: changes in response of colocytes. | patients with inflammatory bowel disease (ibd) have an increased risk of 10%-15% developing colorectal cancer (crc) that is a common disease of high economic costs in developed countries. the crc has been increasing in recent years and its mortality rates are very high. multiple biological and biochemical factors are responsible for the onset and progression of this pathology. moreover, it appears absolutely necessary to investigate the environmental factors favoring the onset of crc and the pro ... | 2014 | 25561781 |
| combining electrochemical sensors with miniaturized sample preparation for rapid detection in clinical samples. | clinical analyses benefit world-wide from rapid and reliable diagnostics tests. new tests are sought with greatest demand not only for new analytes, but also to reduce costs, complexity and lengthy analysis times of current techniques. among the myriad of possibilities available today to develop new test systems, amperometric biosensors are prominent players-best represented by the ubiquitous amperometric-based glucose sensors. electrochemical approaches in general require little and often enoug ... | 2014 | 25558994 |
| combining electrochemical sensors with miniaturized sample preparation for rapid detection in clinical samples. | clinical analyses benefit world-wide from rapid and reliable diagnostics tests. new tests are sought with greatest demand not only for new analytes, but also to reduce costs, complexity and lengthy analysis times of current techniques. among the myriad of possibilities available today to develop new test systems, amperometric biosensors are prominent players-best represented by the ubiquitous amperometric-based glucose sensors. electrochemical approaches in general require little and often enoug ... | 2014 | 25558994 |
| commentary. | 2015 | 25552856 | |
| encapsulation of biocides by cyclodextrins: toward synergistic effects against pathogens. | host-guest chemistry is useful for the construction of nanosized objects. some of the widely used hosts are probably the cyclodextrins (cds). cds can form water-soluble complexes with numerous hydrophobic compounds. they have been widespread used in medicine, drug delivery and are of interest for the biocides encapsulation. indeed, this enables the development of more or less complex systems that release antimicrobial agents with time. in this paper, the general features of cds and their applica ... | 2014 | 25550722 |
| rapid and sensitive detection of listeria ivanovii by loop-mediated isothermal amplification of the smcl gene. | a loop-mediated isothermal amplification (lamp) assay for rapid and sensitive detection of the l. ivanovii strains had been developed and evaluated in this study. oligonucleotide primers specific for l. ivanovii species were designed corresponding to smcl gene sequences. the primers set comprise six primers targeting eight regions on the species-specific gene smcl. the lamp assay could be completed within 1 h at 64°c in a water bath. amplification products were directly observed by the loopamp f ... | 2014 | 25549337 |
| reiter's syndrome and hearing loss: a possible association? | patient complained of hearing loss and tinnitus after the onset of reiter's syndrome. audiometry confirmed the hearing loss on the left ear; blood work showed increased erythrocyte sedimentation rate and c3 fraction of the complement. genotyping for hla-b27 was positive. treatment with prednisolone did not improve the hearing levels. | 2014 | 25548635 |
| cj1386, an atypical hemin-binding protein, mediates hemin trafficking to kata in campylobacter jejuni. | catalase enzymes detoxify h2o2 by the dismutation of h2o2 into o2 and h2o through the use of hemin cofactors. while the structure and biochemical properties of catalase enzymes have been well characterized over many decades of research, it remained unclear how catalases acquire hemin. we have previously reported that cj1386 is essential for ensuring proper hemin content in campylobacter jejuni catalase (kata) (a. flint, y. q. sun, and a. stintzi, j bacteriol 194: 334-345, 2012). in this report, ... | 2014 | 25548249 |
| cj1386, an atypical hemin-binding protein, mediates hemin trafficking to kata in campylobacter jejuni. | catalase enzymes detoxify h2o2 by the dismutation of h2o2 into o2 and h2o through the use of hemin cofactors. while the structure and biochemical properties of catalase enzymes have been well characterized over many decades of research, it remained unclear how catalases acquire hemin. we have previously reported that cj1386 is essential for ensuring proper hemin content in campylobacter jejuni catalase (kata) (a. flint, y. q. sun, and a. stintzi, j bacteriol 194: 334-345, 2012). in this report, ... | 2014 | 25548249 |
| role of the nuclease of nontypeable haemophilus influenzae in dispersal of organisms from biofilms. | nontypeable haemophilus influenzae (nthi) forms biofilms in the middle ear during human infection. the biofilm matrix of nthi contains extracellular dna. we show that nthi possesses a potent nuclease, which is a homolog of the thermonuclease of staphylococcus aureus. using a biofilm dispersal assay, studies showed a biofilm dispersal pattern in the parent strain, no evidence of dispersal in the nuclease mutant, and a partial return of dispersion in the complemented mutant. quantitative pcr of mr ... | 2014 | 25547799 |
| role of the nuclease of nontypeable haemophilus influenzae in dispersal of organisms from biofilms. | nontypeable haemophilus influenzae (nthi) forms biofilms in the middle ear during human infection. the biofilm matrix of nthi contains extracellular dna. we show that nthi possesses a potent nuclease, which is a homolog of the thermonuclease of staphylococcus aureus. using a biofilm dispersal assay, studies showed a biofilm dispersal pattern in the parent strain, no evidence of dispersal in the nuclease mutant, and a partial return of dispersion in the complemented mutant. quantitative pcr of mr ... | 2014 | 25547799 |
| nuclease activity of legionella pneumophila cas2 promotes intracellular infection of amoebal host cells. | legionella pneumophila, the primary agent of legionnaires' disease, flourishes in both natural and man-made environments by growing in a wide variety of aquatic amoebae. recently, we determined that the cas2 protein of l. pneumophila promotes intracellular infection of acanthamoeba castellanii and hartmannella vermiformis, the two amoebae most commonly linked to cases of disease. the cas2 family of proteins is best known for its role in the bacterial and archeal clustered regularly interspaced s ... | 2014 | 25547789 |
| nuclease activity of legionella pneumophila cas2 promotes intracellular infection of amoebal host cells. | legionella pneumophila, the primary agent of legionnaires' disease, flourishes in both natural and man-made environments by growing in a wide variety of aquatic amoebae. recently, we determined that the cas2 protein of l. pneumophila promotes intracellular infection of acanthamoeba castellanii and hartmannella vermiformis, the two amoebae most commonly linked to cases of disease. the cas2 family of proteins is best known for its role in the bacterial and archeal clustered regularly interspaced s ... | 2014 | 25547789 |
| immunoglobulin g fc n-glycosylation in guillain-barré syndrome treated with intravenous immunoglobulin. | 2014 | 25546781 | |
| agricultural and management practices and bacterial contamination in greenhouse versus open field lettuce production. | the aim of this study was to gain insight into potential differences in risk factors for microbial contamination in greenhouse versus open field lettuce production. information was collected on sources, testing, and monitoring and if applicable, treatment of irrigation and harvest rinsing water. these data were combined with results of analysis on the levels of escherichia coli as a fecal indicator organism and the presence of enteric bacterial pathogens on both lettuce crops and environmental s ... | 2014 | 25546272 |
| agricultural and management practices and bacterial contamination in greenhouse versus open field lettuce production. | the aim of this study was to gain insight into potential differences in risk factors for microbial contamination in greenhouse versus open field lettuce production. information was collected on sources, testing, and monitoring and if applicable, treatment of irrigation and harvest rinsing water. these data were combined with results of analysis on the levels of escherichia coli as a fecal indicator organism and the presence of enteric bacterial pathogens on both lettuce crops and environmental s ... | 2014 | 25546272 |
| seasonality and antibiotic resistance of campylobacter in turkish chicken meat. | this study investigated the seasonal prevalence and the antimicrobial susceptibility of campylobacter jejuni and campylobacter coli in 264 samples of chicken meat. the samples encompassed wings (n=88), breasts (n=79) and thighs (n=97) and were purchased from different butcheries and markets in elazig province, in eastern turkey, between december 2009 and november 2010. the meat samples were tested for campylobacter presence and the collected isolates were identified as campylobacter jejuni and c ... | 2014 | 25546065 |
| selective gelatinase inhibition reduces apoptosis and pro-inflammatory immune cell responses in campylobacter jejuni-infected gnotobiotic il-10 deficient mice. | increased levels of the matrix metalloproteinases-2 and -9 (also referred to gelatinase-a and -b, respectively) can be detected in intestinal inflammation. we have recently shown that selective gelatinase blockage by the synthetic compound ro28-2653 ameliorates acute murine ileitis and colitis. we here investigated whether ro28-2653 exerts anti-inflammatory effects in acute campylobacter jejuni-induced enterocolitis of gnotobiotic il-10(-/-) mice generated following antibiotic treatment. mice we ... | 2014 | 25544894 |
| the use of genome wide association methods to investigate pathogenicity, population structure and serovar in haemophilus parasuis. | haemophilus parasuis is the etiologic agent of glässer's disease in pigs and causes devastating losses to the farming industry. whilst some hyper-virulent isolates have been described, the relationship between genetics and disease outcome has been only partially established. in particular, there is weak correlation between serovar and disease phenotype. we sequenced the genomes of 212 isolates of h. parasuis and have used this to describe the pan-genome and to correlate this with clinical and ca ... | 2014 | 25539682 |
| the vibrio cholerae vpra-vprb two-component system controls virulence through endotoxin modification. | the bacterial cell surface is the first structure the host immune system targets to prevent infection. cationic antimicrobial peptides of the innate immune system bind to the membrane of gram-negative pathogens via conserved, surface-exposed lipopolysaccharide (lps) molecules. we recently reported that modern strains of the global intestinal pathogen vibrio cholerae modify the anionic lipid a domain of lps with a novel moiety, amino acids. remarkably, glycine or diglycine addition to lipid a alt ... | 2014 | 25538196 |
| the structures of the colonic mucosa-associated and luminal microbial communities are distinct and differentially affected by a prolonged murine stressor. | the commensal microbiota of the human gastrointestinal tract live in a largely stable community structure, assisting in host physiological and immunological functions. changes to this structure can be injurious to the health of the host, a concept termed dysbiosis. psychological stress is a factor that has been implicated in causing dysbiosis, and studies performed by our lab have shown that restraint stress can indeed shift the cecal microbiota structure as well as increase the severity of a co ... | 2014 | 25536463 |
| a cronobacter turicensis o1 antigen-specific monoclonal antibody inhibits bacterial motility and entry into epithelial cells. | cronobacter turicensis is an opportunistic foodborne pathogen that can cause a rare but sometimes lethal infection in neonates. little is known about the virulence mechanisms and intracellular lifestyle of this pathogen. in this study, we developed an igg monoclonal antibody (mab; mab 2g4) that specifically recognizes the o1 antigen of c. turicensis cells. the antilipopolysaccharide antibody bound predominantly monovalently to the o antigen and reduced bacterial growth without causing cell agglu ... | 2014 | 25534937 |
| a cronobacter turicensis o1 antigen-specific monoclonal antibody inhibits bacterial motility and entry into epithelial cells. | cronobacter turicensis is an opportunistic foodborne pathogen that can cause a rare but sometimes lethal infection in neonates. little is known about the virulence mechanisms and intracellular lifestyle of this pathogen. in this study, we developed an igg monoclonal antibody (mab; mab 2g4) that specifically recognizes the o1 antigen of c. turicensis cells. the antilipopolysaccharide antibody bound predominantly monovalently to the o antigen and reduced bacterial growth without causing cell agglu ... | 2014 | 25534937 |
| rapid and accurate detection of bacteriophage activity against escherichia coli o157:h7 by propidium monoazide real-time pcr. | conventional methods to determine the efficacy of bacteriophage (phage) for biocontrol of e. coli require several days, due to the need to culture bacteria. furthermore, cell surface-attached phage particles may lyse bacterial cells during experiments, leading to an overestimation of phage activity. dna-based real-time quantitative polymerase chain reaction (qpcr) is a fast, sensitive, and highly specific means of enumerating pathogens. however, qpcr may underestimate phage activity due to its i ... | 2014 | 25530959 |
| toll-like receptors recognize distinct proteinase-resistant glycoconjugates in campylobacter jejuni and escherichia coli. | campylobacter jejuni causes gastroenteritis and autoimmune neuropathy guillain-barré syndrome. the mechanism by which c. jejuni infection results in such the hyperimmunity is not completely understood. host immunity plays an important role in the disease pathogenesis; however, little is known how immune system recognizes this human pathogen. in this study, we report that toll-like receptors recognize distinct proteinase k-resistant glycoconjugates in c. jejuni and escherichia coli. lipopolysacch ... | 2015 | 25530156 |
| molecular characterization of a homolog of the ferric-uptake regulator, fur, from the marine bacterium marinobacter algicola dg893. | full length recombinant iron regulatory protein, fur, has been isolated and characterized from the algal-associated marine bacterium marinobacter algicola dg893. under nondenaturing conditions the fur protein behaves on size exclusion chromatography as a dimer while it is monomeric under sds page conditions. icp-ms and fluorescence quenching experiments show that mb-fur binds a single metal ion (zn, mn, or co) per monomer. electrophoretic mobility shift assays were used to probe the interaction ... | 2014 | 25528647 |
| molecular characterization of a homolog of the ferric-uptake regulator, fur, from the marine bacterium marinobacter algicola dg893. | full length recombinant iron regulatory protein, fur, has been isolated and characterized from the algal-associated marine bacterium marinobacter algicola dg893. under nondenaturing conditions the fur protein behaves on size exclusion chromatography as a dimer while it is monomeric under sds page conditions. icp-ms and fluorescence quenching experiments show that mb-fur binds a single metal ion (zn, mn, or co) per monomer. electrophoretic mobility shift assays were used to probe the interaction ... | 2014 | 25528647 |
| oligosaccharide composition of breast milk influences survival of uninfected children born to hiv-infected mothers in lusaka, zambia. | human milk oligosaccharides (hmos) have multiple immunomodulatory functions that influence child health. | 2014 | 25527660 |
| oligosaccharide composition of breast milk influences survival of uninfected children born to hiv-infected mothers in lusaka, zambia. | human milk oligosaccharides (hmos) have multiple immunomodulatory functions that influence child health. | 2014 | 25527660 |
| nod1 and nod2: signaling, host defense, and inflammatory disease. | the nucleotide-binding oligomerization domain (nod) proteins nod1 and nod2, the founding members of the intracellular nod-like receptor family, sense conserved motifs in bacterial peptidoglycan and induce proinflammatory and antimicrobial responses. here, we discuss recent developments about the mechanisms by which nod1 and nod2 are activated by bacterial ligands, the regulation of their signaling pathways, and their role in host defense and inflammatory disease. several routes for the entry of ... | 2014 | 25526305 |
| dimeric structure of the bacterial extracellular foldase prsa. | secretion of proteins into the membrane-cell wall space is essential for cell wall biosynthesis and pathogenicity in gram-positive bacteria. folding and maturation of many secreted proteins depend on a single extracellular foldase, the prsa protein. prsa is a 30-kda protein, lipid anchored to the outer leaflet of the cell membrane. the crystal structure of bacillus subtilis prsa reveals a central catalytic parvulin-type prolyl isomerase domain, which is inserted into a larger composite nc domain ... | 2014 | 25525259 |
| dimeric structure of the bacterial extracellular foldase prsa. | secretion of proteins into the membrane-cell wall space is essential for cell wall biosynthesis and pathogenicity in gram-positive bacteria. folding and maturation of many secreted proteins depend on a single extracellular foldase, the prsa protein. prsa is a 30-kda protein, lipid anchored to the outer leaflet of the cell membrane. the crystal structure of bacillus subtilis prsa reveals a central catalytic parvulin-type prolyl isomerase domain, which is inserted into a larger composite nc domain ... | 2014 | 25525259 |
| chagas' disease: an emergent urban zoonosis. the caracas valley (venezuela) as an epidemiological model. | the unprecedented emergence of important public health and veterinary zoonoses is usually a result of exponential population growth and globalization of human activities. i characterized chagas' disease as an emergent zoonosis in the caracas valley (venezuela) due to the following findings: the presence of reservoirs (didelphis marsupialis, rattus rattus) and vectors (panstrongylus geniculatus, panstrongylus rufotuberculatus) infected with trypanosoma cruzi in urbanized or marginalized areas; th ... | 2014 | 25520950 |
| reversal of igm deficiency following a gluten-free diet in seronegative celiac disease. | selective igm deficiency (sigmd) is very rare; it may be associated with celiac disease (cd). we present the case of an 18-year-old man with sigmd masking seronegative cd. symptoms included abdominal pain, diarrhea and weight loss. laboratory tests showed reduced igm, dq2-hla and negative anti-transglutaminase. villous atrophy and diffuse immature lymphocytes were observed at histology. tissue transglutaminase mrna mucosal levels showed a 6-fold increase. the patient was treated with a gluten-fr ... | 2014 | 25516687 |
| the aeromonas caviae aha0618 gene modulates cell length and influences swimming and swarming motility. | aeromonas caviae is motile via a polar flagellum in liquid culture, with a lateral flagella system used for swarming on solid surfaces. the polar flagellum also has a role in cellular adherence and biofilm formation. the two subunits of the polar flagellum, flaa and flab, are posttranslationally modified by o-linked glycosylation with pseudaminic acid on 6-8 serine and threonine residues within the central region of these proteins. this modification is essential for the formation of the flagellu ... | 2014 | 25515520 |
| the aeromonas caviae aha0618 gene modulates cell length and influences swimming and swarming motility. | aeromonas caviae is motile via a polar flagellum in liquid culture, with a lateral flagella system used for swarming on solid surfaces. the polar flagellum also has a role in cellular adherence and biofilm formation. the two subunits of the polar flagellum, flaa and flab, are posttranslationally modified by o-linked glycosylation with pseudaminic acid on 6-8 serine and threonine residues within the central region of these proteins. this modification is essential for the formation of the flagellu ... | 2014 | 25515520 |
| the b-type channel is a major route for iron entry into the ferroxidase center and central cavity of bacterioferritin. | bacterioferritin is a bacterial iron storage and detoxification protein that is capable of forming a ferric oxyhydroxide mineral core within its central cavity. to do this, iron must traverse the bacterioferritin protein shell, which is expected to occur through one or more of the channels through the shell identified by structural studies. the size and negative electrostatic potential of the 24 b-type channels suggest that they could provide a route for iron into bacterioferritin. residues at t ... | 2014 | 25512375 |
| the b-type channel is a major route for iron entry into the ferroxidase center and central cavity of bacterioferritin. | bacterioferritin is a bacterial iron storage and detoxification protein that is capable of forming a ferric oxyhydroxide mineral core within its central cavity. to do this, iron must traverse the bacterioferritin protein shell, which is expected to occur through one or more of the channels through the shell identified by structural studies. the size and negative electrostatic potential of the 24 b-type channels suggest that they could provide a route for iron into bacterioferritin. residues at t ... | 2014 | 25512375 |
| genotypes and antibiotic resistance of bovine campylobacter and their contribution to human campylobacteriosis. | campylobacter jejuni and campylobacter coli are the most important bacterial causes of human gastroenteritis. chicken has been recognized as a major source for human infection, whereas cattle might also contribute to a lesser extent. however, there is a paucity of information available regarding campylobacter in swiss cattle and their role for human campylobacteriosis. to gain more information on genotypes and antibiotic resistance of bovine c. jejuni and c. coli and on their contribution to hum ... | 2015 | 25511436 |
| factors predicting poor outcome in patients with fulminant guillaine-barré syndrome. | this paper describes three patients with acute fulminant guillain-barrι syndrome (gbs) with electrophysiologically inexcitable peripheral nerves not responding to two courses of intravenous immunoglobulin. their clinical profile is compared with two other gbs patients having similar severity of disease but with demyelinative features, managed similarly during the same period. patients who failed to respond were elderly with a mean age of 60 years, had prodromal diarrhea, rapid progression of mus ... | 2014 | 25506175 |
| helical shape of helicobacter pylori requires an atypical glutamine as a zinc ligand in the carboxypeptidase csd4. | peptidoglycan modifying carboxypeptidases (cps) are important determinants of bacterial cell shape. here, we report crystal structures of csd4, a three-domain protein from the human gastric pathogen helicobacter pylori. the catalytic zinc in csd4 is coordinated by a rare his-glu-gln configuration that is conserved among most csd4 homologs, which form a distinct subfamily of cps. substitution of the glutamine to histidine, the residue found in prototypical zinc carboxypeptidases, resulted in decr ... | 2014 | 25505267 |
| helical shape of helicobacter pylori requires an atypical glutamine as a zinc ligand in the carboxypeptidase csd4. | peptidoglycan modifying carboxypeptidases (cps) are important determinants of bacterial cell shape. here, we report crystal structures of csd4, a three-domain protein from the human gastric pathogen helicobacter pylori. the catalytic zinc in csd4 is coordinated by a rare his-glu-gln configuration that is conserved among most csd4 homologs, which form a distinct subfamily of cps. substitution of the glutamine to histidine, the residue found in prototypical zinc carboxypeptidases, resulted in decr ... | 2014 | 25505267 |
| impact of the lab-score on antibiotic prescription rate in children with fever without source: a randomized controlled trial. | the lab-score, based on the combined determination of procalcitonin, c-reactive protein and urinary dipstick results, has been shown accurate in detecting serious bacterial infections (sbi) in children with fever without source (fws) on retrospective cohorts. we aimed to prospectively assess the utility of the lab-score in safely decreasing antibiotic prescriptions in children with fws and to determine its diagnostic characteristics compared to common sbi biomarkers. | 2014 | 25503770 |
| innate endogenous adjuvants prime to desirable immune responses via mucosal routes. | vaccination is an effective strategy to prevent infectious or immune related diseases, which has made remarkable contribution in human history. recently increasing attentions have been paid to mucosal vaccination due to its multiple advantages over conventional ways. subunit or peptide antigens are more reasonable immunogens for mucosal vaccination than live or attenuated pathogens, however adjuvants are required to augment the immune responses. many mucosal adjuvants have been developed to prim ... | 2014 | 25503634 |
| innate endogenous adjuvants prime to desirable immune responses via mucosal routes. | vaccination is an effective strategy to prevent infectious or immune related diseases, which has made remarkable contribution in human history. recently increasing attentions have been paid to mucosal vaccination due to its multiple advantages over conventional ways. subunit or peptide antigens are more reasonable immunogens for mucosal vaccination than live or attenuated pathogens, however adjuvants are required to augment the immune responses. many mucosal adjuvants have been developed to prim ... | 2014 | 25503634 |
| prevalence of type vi secretion system in spanish campylobacter jejuni isolates. | infections from campylobacter jejuni pose a serious public health problem and are now considered the leading cause of foodborne bacterial gastroenteritis throughout the world. sequencing of c. jejuni genomes has previously allowed a number of loci to be identified, which encode virulence factors that aid survival and pathogenicity. recently, a type vi secretion system (t6ss) consisting of 13 conserved genes was described in c. jejuni strains and recognised to promote pathogenicity and adaptation ... | 2015 | 25496466 |
| use of in vivo induced antigen technology to identify genes from aeromonas salmonicida subsp. salmonicida that are specifically expressed during infection of the rainbow trout oncorhynchus mykiss. | aeromonas salmonicida is a major fish pathogen associated with mass mortalities in salmonid fish. in the present study, we applied in vivo induced antigen technology (iviat), a technique that relies on antibodies adsorbed against in vitro cultures of the pathogen, to a clinical isolate of a. salmonicida subsp. salmonicida. | 2014 | 25495705 |
| untargeted metabolomic profiling of amphenicol-resistant campylobacter jejuni by ultra-high-performance liquid chromatography-mass spectrometry. | campylobacter jejuni, an important foodborne microorganism, poses severe and emergent threats to human health as antibiotic resistance becomes increasingly prevalent. the mechanisms of drug resistance are hard to decipher, and little is known at the metabolic level. here we apply metabolomic profiling to discover metabolic changes associated with amphenicol (chloramphenicol and florfenicol) resistance mutations of campylobacter jejuni. an optimized sample preparation method was combined with ult ... | 2015 | 25491530 |
| [acute diarrheal disease caused by enteropathogenic escherichia coli in colombia]. | intestinal escherichia coli pathogens are leading causes of acute diarrheal disease in children less than 5 years in latin america, africa and asia and a leading cause of death in children living in poorest communities in africa and south east asia. studies on the role of e. coli pathogens in childhood diarrhea in colombia and other countries in latin america are limited due to the lack of detection assays in clinical laboratories at the main urban medical centers. recent studies report that ent ... | 2014 | 25491457 |
| reduction of campylobacter jejuni in broiler chicken by successive application of group ii and group iii phages. | bacteriophage treatment is a promising tool to reduce campylobacter in chickens. several studies have been published where group ii or group iii phages were successfully applied. however, these two groups of phages are different regarding their host ranges and host cell receptors. therefore, a concerted activity of group ii and group iii phages might enhance the efficacy of a treatment and decrease the number of resistant bacteria. | 2014 | 25490713 |
| transfection of eimeria mitis with yellow fluorescent protein as reporter and the endogenous development of the transgenic parasite. | advancements have been made in the genetic manipulation of apicomplexan parasites. both the in vitro transient and in vivo stable transfection of eimeria tenella have been developed successfully. herein, we report the transient and stable transfection of eimeria mitis. | 2014 | 25490541 |