Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| characterization of the genes encoding beta-ketoadipate: succinyl-coenzyme a transferase in pseudomonas putida. | beta-ketoadipate:succinyl-coenzyme a transferase (beta-ketoadipate:succinyl-coa transferase) (ec 2.8.3.6) carries out the penultimate step in the conversion of benzoate and 4-hydroxybenzoate to tricarboxylic acid cycle intermediates in bacteria utilizing the beta-ketoadipate pathway. this report describes the characterization of a dna fragment from pseudomonas putida that encodes this enzyme. the fragment complemented mutants defective in the synthesis of the coa transferase, and two proteins of ... | 1992 | 1624453 |
| colonization of the small intestine of weaned pigs by enterotoxigenic escherichia coli that lack known colonization factors. | intestinal colonization of 3-week-old weaned pigs by enterotoxigenic escherichia coli (etec) strains that were originally isolated from weaned pigs with fatal diarrhea and that lacked k88, k99, f41, and 987p adhesins (4p- etec) was studied by histologic, immunofluorescent, and electron microscopic techniques. in the first experiment, 16 principal pigs were inoculated orogastrically with etec strain 2134 (serogroup o157: h19) or 2171 (serogroup 0141:h4), and eight control pigs were not inoculated ... | 1992 | 1621335 |
| time course of various inflammatory mediators during recurrent endotoxemia. | the time course of thromboxane b2 (txb2), 6-keto-pgf1 alpha (stable metabolite of prostacyclin), tumor necrosis factor-alpha (tnf alpha), platelet activating factor (paf), and interleukin-6 (il-6) formation after three lipopolysaccharide (lps) infusions was studied in pigs over an 18-hr, period. the escherichia coli endotoxin w0111:b4 was injected i.v. into 10 of the test group pigs at a dose of 0.5 micrograms/kg over 30 min at 0, 5 and 10 hr of the experiment. three pigs injected with physiolog ... | 1992 | 1599496 |
| influence of indomethacin on endotoxin-induced changes in gastrointestinal myoelectrical activity and some haematological and clinical parameters in the conscious piglet. | the effect of indomethacin, administered intravenously at 5 mg/kg, on the changes in gastrointestinal myoelectrical activity, rectal body temperature, clinical appearance and some haematological parameters induced by intravenous bolus injection of endotoxin, at 10 micrograms/kg, was examined in conscious piglets with electrodes implanted in the antrum pylori, duodenum, jejunum and ileum. indomethacin inhibited the endotoxin-induced febrile response and the accompanying clinical signs. however, i ... | 1992 | 1598755 |
| 31p nmr spectroscopic studies on purified, native and cloned, expressed forms of nadph-cytochrome p450 reductase. | 31p nmr spectroscopy has been utilized in conjunction with site-directed mutagenesis and phospholipid analysis to determine structural aspects of the prosthetic flavins, fad and fmn, of nadph-cytochrome p450 reductase. comparisons are made among detergent-solubilized and protease (steapsin)-solubilized preparations of porcine liver reductases, showing unequivocally that the 31p nmr signals at approximately 0.0 ppm in the detergent-solubilized, hydrophobic form are attributable to phospholipids. ... | 1992 | 1567869 |
| characterization of escherichia coli strains isolated from pigs with edema disease. | escherichia coli strains belonging to serogroups o 138 and o 139 isolated from pigs with edema disease, were characterized with respect to the presence of genes encoding shiga-like toxin i, shiga-like toxin ii and shiga-like toxin iiv (slt i, slt ii and slt iiv). genes coding for the heat-stable and heat-labile enterotoxins (st i and lt i) were also detected. plasmid profiling, restriction enzyme digestion of total dna, and ribotyping were performed for further characterization of the strains. t ... | 1992 | 1557896 |
| expression in vivo and in vitro of the major structural protein (vp73) of african swine fever virus. | the vp73 protein was produced by in vitro transcription and translation from the xho i-bam hi fragment located between the cla i-n and cla i-h fragments of the viral genome. this dna fragment encodes a late mrna of about 2.6 kb detected in infected ms monkey and bhk hamster cells. the transcript was initiated at a site within two bases upstream of the translation initiation codon. the in vitro synthesized polypeptide shows the same molecular weight as the in vivo synthesized polypeptide, suggest ... | 1992 | 1550491 |
| expression of porcine complement cytolysis inhibitor mrna in cultured aortic smooth muscle cells. changes during differentiation in vitro. | porcine smooth muscle cells (smc) grown to a high density monolayer culture undergo a morphological transition in which the cells draw away from the substrate and form multicellular nodules. the cells within the nodule resemble smc in the aortic media and in some atherosclerotic plaques. the process of nodule formation is associated with the enhanced production of a secreted 38-kda glycoprotein. to characterize the 38-kda protein and its expression, a cdna clone (pc38k) was isolated by immunolog ... | 1992 | 1544909 |
| cloning and expression of a transferrin-binding protein from actinobacillus pleuropneumoniae. | an expression library was constructed from actinobacillus pleuropneumoniae serotype 7. escherichia coli transformants expressing recombinant proteins were identified by immunoscreening with porcine convalescent serum. one transformant expressing a 60-kda protein (60k protein) in aggregated form was identified. serum raised against the recombinant protein recognized a polypeptide with an indistinguishable electrophoretic mobility in the a. pleuropneumoniae wild type after iron-restricted growth o ... | 1992 | 1541562 |
| is escherichia coli invading tubuloepithelial cells? | the adhesion of escherichia coli to host epithelium cells is the very first step of urinary tract infections followed by the internalization of the bacteria into these cells. these steps are influenced by several surface antigens or products of the pathogen, e.g. fimbriae or adhesins, k antigen, and hemolysin. the bacterial adherence and the internalization of several isogenic e. coli o18 strains differing in the expression of k5 antigen, hemolysin, and type of fimbriae were measured by using a ... | 1992 | 1520978 |
| fate of escherichia coli o157:h7 as affected by ph or sodium chloride and in fermented, dry sausage. | the influence of ph adjusted with lactic acid or hcl or sodium chloride concentration on survival or growth of escherichia coli o157:h7 in trypticase soy broth (tsb) was determined. studies also determined the fate of e. coli o157:h7 during the production and storage of fermented, dry sausage. the organism grew in tsb containing less than or equal to 6.5% nacl or at a ph of 4.5 to 9.0, adjusted with hcl. when tsb was acidified with lactic acid, the organism grew at ph 4.6 but not at ph 4.5. a co ... | 1992 | 1514799 |
| polymerase chain reaction for detection of verocytotoxigenic escherichia coli isolated from animal and food sources. | animals and their by-products have been implicated as important sources of verocytotoxigenic escherichia coli (vtec) associated with disease in humans. vtec comprise a wide range of serotypes and produce a variety of closely related verocytotoxins (vt). a pair of oligonucleotide primers, targeting conserved sequences found in vt1, vt2 and vte genes, was used to develop a polymerase chain reaction (pcr) procedure to detect all types of vtec. supernatants of boiled broth cultures of vtec (223 stra ... | 1992 | 1513344 |
| solubilization of growth hormone and other recombinant proteins from escherichia coli inclusion bodies by using a cationic surfactant. | recombinant pig growth hormone (rpgh) was solubilized from inclusion bodies by using the cationic surfactant cetyltrimethylammonium chloride (ctac). the solubilizing action of ctac appeared to be dependent on the presence of a positively charged head group, as a non-charged variant was inactive. relatively low concentrations of ctac were required for rapid solubilization, and protein-bound ctac was easily removed by ion-exchange chromatography. compared with solubilization and recovery of rpgh f ... | 1992 | 1497625 |
| effects of injectable beta-carotene and vitamin a on lymphocyte proliferation and polymorphonuclear neutrophil function in piglets. | newborn piglets were injected with either (1) vehicle; (2) 20 mg beta-carotene; (3) 40 mg beta-carotene; (4) 25,000 iu vitamin a palmitate, or (5) 50,000 iu vitamin a palmitate. blood was collected at 0.5, 1, 3, and 6 weeks of age and lymphocyte proliferation, polymorphonuclear neutrophil (pmn) phagocytosis and intracellular killing of live escherichia coli were measured. lymphocyte proliferation in all piglets was high at birth but decreased thereafter. piglets injected with either high beta-ca ... | 1992 | 1467371 |
| molecular characterisation of a dna ligase gene of the extremely thermophilic archaeon desulfurolobus ambivalens shows close phylogenetic relationship to eukaryotic ligases. | a 3382 bp fragment containing a gene for a dna ligase from the extremely thermophilic, acidophilic, and facultatively anaerobic archaeon (archaebacterium) desulfurolobus ambivalens was cloned and sequenced. the deduced amino acid sequence (600 amino acids, 67619 molecular weight) showed 30-34% sequence identity with the atp-dependent eucaryal (eukaryotic) dna ligases of schizosaccharomyces pombe, saccharomyces cerevisiae, the human dna ligase i, and with the vaccinia dna ligase. distant similari ... | 1992 | 1437556 |
| [isolation of vero-cytotoxin-producing escherichia coli from cattle and serotyping and toxin-typing of the isolated strains]. | two hundred and sixty-six piglets with diarrhea (from 4 farms), 73 healthy pregnant pig (from 2 farms), 27 calves with diarrhea (from 9 farms) and 47 healthy milk cows (from 1 farm) were examined for vero-cytotoxin-producing escherichia coli (vtec), and 52, 11, 15 and 67 strains of vtec were isolated from 17 piglets, 11 pregnant pigs, 6 calves and 23 milk cows, respectively. all vtec strains from the piglets produced only vt2vp, while the strains from the healthy pigs did not produce vt2vp, but ... | 1992 | 1431372 |
| effect of concentration and source of sulfate on nursery pig performance and health. | the effect of sulfate in drinking water at concentrations of 600, 1,200, and 1,800 mg/l on nursery pig performance and health was evaluated over 28 days on 415 weaned pigs. sodium sulfate and magnesium sulfate were evaluated in combination at concentrations of 600, 1,200, and 1,800 mg/l, and independently at concentrations of 600 and 1,800 mg/l in the drinking water. seven treatment groups and 1 control group were evaluated for mean gain, feed consumption, water consumption, feed conversion, pre ... | 1992 | 1429159 |
| lipopolysaccharide (lps) inhalation in healthy subjects increases neutrophils, lymphocytes and fibronectin levels in bronchoalveolar lavage fluid. | bacterial endotoxin has been suggested as responsible for the development of subjective symptoms and transient or chronic lung function impairment seen after exposure to organic dusts in cotton mills, poultry houses, swine confinement buildings and saw mills. animal experiments have demonstrated bronchoalveolar neutrophilia being the most prominent cell response in animals following bacterial lipopolysaccharide (lps) inhalation. the present study was conducted to obtain information on some aspec ... | 1992 | 1426208 |
| observations on production of hemolysin, heat-labile enterotoxin and antimicrobial drug resistance among enterotoxigenic escherichia coli from pigs. | a total of 52 isolates of escherichia coli belonging to enterotoxigenic serotypes from piglets with diarrhea were examined for hemolysis, production of cholera-like enterotoxin (lt) and susceptibility to 10 antimicrobial drugs. a strong association between production of lt and hemolysis was seen. ninety percent of 29 hemolylic isolates were lt+ whereas 100% of 23 nonhemolytic isolates were lt- in a commercial latex agglutination assay. antimicrobial susceptibility tests employing disc diffusion ... | 1992 | 1424558 |
| evaluation of three different stb assays and comparison of enterotoxin pattern over a five-year period in swedish porcine escherichia coli. | the pig intestinal loop (pil) assay, inhibition enzyme-linked immunosorbent assay (elisa), and dna hybridization assay were compared for analysis of escherichia coli heat-stable enterotoxin b (stb) on 201 porcine e. coli strains. the dna hybridization had a 95% correlation with the stb elisa and was therefore chosen as the method for subsequent screening of enterotoxin genes: heat labile (lt), heat-stable a (sta), and/or stb. in contrast to the pil assay, both the stb elisa and dna hybridization ... | 1992 | 1424503 |
| identification of shiga-like toxin type ii producing escherichia coli using the polymerase chain reaction and a digoxigenin labelled dna probe. | epidemiological studies have demonstrated that enterohaemorrhagic strains of escherichia coli which cause the haemolytic uremic syndrome in humans and the oedema disease in pigs more frequently produce shiga-like toxin type ii (slt-ii) than any other member of the shiga-like toxin family. a technique has been developed for the identification of slt-ii producing e. coli using the polymerase chain reaction (pcr) and a digoxigenin (dig)-labelled dna probe to facilitate the early detection and epide ... | 1992 | 1406729 |
| influence of actinobacillus pleuropneumoniae serotype 2 and its cytolysins on porcine neutrophil chemiluminescence. | the effects of actinobacillus pleuropneumoniae serotype 2 and its metabolites on the oxidative activity of porcine neutrophils were studied by using a chemiluminescence technique. viable a. pleuropneumoniae stimulated the production of oxygen radicals by neutrophils. after having reached a peak value, the oxidative activity decreased until a total inhibition of the oxidative activity of the neutrophils was achieved. all effects were neutralized with homologous convalescent-phase pig sera which h ... | 1992 | 1398943 |
| heterogeneity among heat-labile enterotoxins produced by porcine enterotoxigenic escherichia coli. | the heat-labile enterotoxins (lt) produced by various porcine strains (ltp) of enterotoxigenic escherichia coli were purified to homogeneity and their molecular properties were compared with each other. by double gel diffusion and rabbit skin permeability test, ltps from wt-1 (ltp-wt-1) and bo-149 (ltp-bo-149) strains were antigenically and biologically identical. by polyacrylamide gel electrophoresis with sodium dodecyl sulfate (sds), the mobilities of a and b subunits of ltp (bo-149) were iden ... | 1992 | 1397223 |
| a chromosome 1-specific dna library from the domestic pig (sus scrofa domestica). | chromosomes were prepared from lymphocytes of a male domestic pig and flow-sorted on a dual-laser facs. twenty spots were observed, corresponding to the known pig karyotype of 18 pairs of autosomes plus the x and y. dna was isolated from 10,000 copies of the presumed chromosome 1 spot, restricted with sau3a, ligated into the vector pgem4z, and pcr amplified using universal primers; the products were then re-ligated into puc18. after transformation into escherichia coli, 210,000 independent colon ... | 1992 | 1395721 |
| cloning and expression of the gal beta 1, 3galnac alpha 2,3-sialyltransferase. | sequence information obtained by nh2-terminal sequence analysis of two molecular weight forms (45 and 48 kda) of the porcine gal beta 1,3galnac alpha 2,3-sialyltransferase was used to clone a full-length cdna of the enzyme. the cdna sequence revealed an open reading frame coding for 343 amino acids and a putative domain structure consisting of a short nh2-terminal cytoplasmic domain, a signal-anchor sequence, and a large cooh-terminal catalytic domain. this domain structure was confirmed by cons ... | 1992 | 1383214 |
| location of the cytoplasmic epitope for a k(+)-competitive antibody of the (h+,k+)-atpase. | the monoclonal antibody (mab) 95-111 binds the alpha subunit of (h+,k+)-atpase and inhibits the k(+)-atpase activity. to map the epitope, all of the partial sequences of the alpha subunit were expressed in escherichia coli hb101 using rabbit alpha subunit cdna restriction fragments ligated into puex vector. bacterial recombinant lysates were separated by sodium dodecyl sulfate-gel electrophoresis, and the epitope was detected by western blotting. the antibody site was mapped between cys529 and g ... | 1992 | 1382067 |
| evidence for linkage between the swine l blood group and the loci specifying the receptors mediating adhesion of k88 escherichia coli pilus antigens. | brush borders or enterocytes obtained from the small intestine of 248 pedigreed pigs were tested by adhesion assay in vitro with enterotoxigenic escherichia (e.) coli strains, each expressing one of the three k88 pilus variants k88ab, k88ac and k88ad. all pigs were classified as belonging to one of the four adhesion phenotypes: i--k88ab(-), ac(-), ad(-); ii--k88ab(-), ac(-), ad(+); iii--k88ab(+), ac(+), ad(-); and iv--k88ab(+), ac(+), ad(+). serum or red cells were typed for 15 blood group syste ... | 1992 | 1349202 |
| restriction fragment length polymorphism and virulence pattern of the veterinary pathogen escherichia coli o139:k82:h1. | escherichia coli o139:k82:h1 strains originating from outbreaks and single cases of oedema disease in pigs were characterized by their genomic restriction fragment length polymorphism (rflp), their virulence pattern, and by the occurrence as well as the genomic distribution of the determinants for hemolysin (hly) and verotoxins (shiga-like toxins; slti, sltii). whereas the rflps revealed considerable variation among the e. coli o139:k82:h1 isolates depending the origin and epidemic source of the ... | 1992 | 1348438 |
| [remember escherichia coli mutabile]. | 1992 | 1340254 | |
| susceptibility to antibiotics of escherichia coli strains isolated from diarrhoeic and non-diarrhoeic livestock in trinidad. | the sensitivity of strains of escherichia coli isolated from calves, piglets, lambs and kids in trinidad to seven antibiotics was determined. two hundred and sixty-four (91.3%) of 289 strains isolated from diarrhoeic animals and 173 (87.4%) of 198 strains from non-diarrhoeic animals exhibited resistance to one or more antibiotics. the difference was not statistically significant (p > or = 0.05; x2). regardless of health status, isolates from lambs were least resistant (75.0%) and those from pigl ... | 1992 | 1339991 |
| mutational analysis of active site residues in pig heart aconitase. | a cdna encoding mature porcine heart aconitase was over-expressed in escherichia coli under the control of a phage t7 promoter. recombinant aconitase purified from e. coli was identical to the enzyme from pig and beef heart in size, [3fe-4s] and [4fe-4s] cluster structure and enzymatic activity. nine amino acid residues in close proximity to the fe-s cluster and bound substrate (lauble, h., kennedy, m.c., beinert, h., and stout, c.d. (1992) biochemistry, in press) were replaced by site-directed ... | 1992 | 1313811 |
| isolation and sequence analysis of the cdna for pig kidney fructose 1,6-bisphosphatase. | a full-length clone of pig kidney fructose 1,6-bisphosphatase (d-fructose-1,6-bisphosphate 1-phosphohydrolase, ec 3.1.3.11) was isolated by screening a cdna library for complementation of an escherichia coli fbp deletion mutation. the open reading frame of 1011 bases corresponds to 337 amino acids, two more than have been previously reported [marcus, f., edelstein, i., reardon, i. & heinrikson, r. l. (1982) proc. natl. acad. sci. usa 79, 7161-7165]. the extra two amino acids (ala-lys) are locate ... | 1992 | 1313579 |
| production in escherichia coli of porcine type-i collagenase as a fusion protein with beta-galactosidase. | porcine type-i collagenase (colg-1) was produced as a fusion protein in escherichia coli using the pax5 expression vector. the fusion protein consists of beta-galactosidase at the n terminus joined to a collagen hinge region and a blood-coagulation factor xa cleavage site linked to colg-1. recombinant collagenase (recolg-1) was biologically active in the form of a fusion protein and could be released by treatment with factor xa to yield colg-1 with the authentic n terminus (phenylalanine) found ... | 1992 | 1311701 |
| a ubiquitin conjugating enzyme encoded by african swine fever virus. | the post-translational modification of proteins by covalent attachment of ubiquitin occurs in all eukaryotes by a multi-step process. a family of e2 or ubiquitin conjugating (ubc) enzymes catalyse one step of this process and these have been implicated in several diverse regulatory functions. we report here the sequence of a gene encoded by african swine fever virus (asfv) which has high homology with ubc enzymes. this asfv encoded enzyme has ubc activity when expressed in escherichia coli since ... | 1992 | 1310934 |
| [expression of recombinant human hemoglobin]. | the well recognized prevalence of infectious agents in products derived from human whole blood and the increasing number of transfusion-transmitted diseases has made urgent the search for a safe alternative to conventional blood transfusion. sources of hemoglobin (hb) different from outdated human bank blood are under active scrutiny in several laboratories. different approaches have been proposed to produce recombinant human hb in bacteria (e. coli), yeast (s. cerevisiae) and transgenic mammals ... | 1992 | 1288539 |
| nucleotide sequence of cdna for porcine heme oxygenase and its expression in escherichia coli. | the nucleotide sequence of a cdna for porcine heme oxygenase was determined. the open reading frame encoded a polypeptide of 288 amino acid residues with a molecular mass of 33,074 da. a prokaryotic expression plasmid carrying porcine heme oxygenase cdna was constructed and transfected into escherichia coli cells. the full-length heme oxygenase expressed was localized in the bacterial membranes. two small-sized heme oxygenases with no membrane-bound properties were also detected, suggesting that ... | 1992 | 1288499 |
| isolation and characterization of adhesin-defective tnphoa mutants of septicaemic porcine escherichia coli of serotype o115:k-:f165. | non-enterotoxigenic porcine escherichia coli strains belonging to the serogroup o115 have been associated with septicaemia and diarrhoea. putative factors important in the pathogenicity of e. coli of serogroup o115 include fimbrial antigen f165, haemagglutination (mrha), lipopolysaccharide, serum resistance, capsule and production of aerobactin. using tnphoa transposon insertion mutagenesis, two classes of mutants were obtained from e. coli of serotype o115:f165 with respect to the phenotypic ex ... | 1992 | 1282536 |
| no accounts completely for the oxygenated nitrogen species generated by enzymic l-arginine oxygenation. | we have assessed the stoichiometry of the nitric oxide (no) synthase reaction by using a novel e.p.r. technique. no generated by crude and partially purified no synthase from endothelial cells and escherichia coli-lipopolysaccharide-activated macrophages was trapped by a ferrous diethyldithiocarbamate complex dispersed in yeast. the paramagnetic ferrous mononitrosyl dithiocarbamate complex formed exhibited a characteristic e.p.r. signal at g perpendicular = 2.035 and g parallel = 2.02 with a tri ... | 1992 | 1281408 |
| isolation and metabolism cage system for newborn pigs. | an isolation system was designed and constructed for isolating normal and infected newborn pigs. the system consisted of an outer cage fitted with a biological diffusion filter and a dunk bath entry system and an inner metabolism cage to contain the pig. when tested with s-13 bacteriophage, the isolation and metabolism cage system was at least 99% efficient in preventing the entry or escape of microorganism. a total of 267 escherichia coli-infected newborn pigs have been isolated in these units, ... | 1976 | 1259227 |
| the etiology of swine dysentery. iii. the role of selected gram-negative obligate anaerobes. | a disease resembling swinee dysentery occurred in gnotobiotic piglets previously colonized with escherichia coli and given a mixture of five enteric anaerobes and spirochete b-78, orally. no disease occurred in germ-free piglets exposed to four gram-negative obligate anaerobes or to the spirochete; however, if the four respective anaerobes were introduced into gnotobiotic pigs previously colonized with the spirochete, or when the spirochete was introduced into gnotobiotic pigs colonized with the ... | 1975 | 1167199 |
| [diagnosis of edema disease (colienterotoxemia) in piglets]. | 1975 | 1108384 | |
| [protective oral immunization against coli enterotoxemia in swine (author's tranls)]. | the issue of immunity in the case of enteric infections caused by bacteria is still widely unresolved. to clarify this, weaned piglets 6-7 weeks of age were used as a model, because of the particular susceptibility to colienterotoxemia shown by them at this particular age. the experimental design was as follows. the animals were immunized orally and parenterally (i.v. and i.p. routes) by means of a vaccine consisting of bacteria incapable of reproduction. 14 days later, the animals were challeng ... | 1975 | 1106063 |
| [serotype 185 and e. coli o115 - two distinct bioseropathotypes (author's transl)]. | e. coli o115 strain 27w and trabulsi's serotype 185 have been subjected by us to comparing examinations of the serological behaviour of their cell wall and their protoplasmatic antigens, of their biochemical and cultural behaviour and their pathogenicity for the mucous membranes of guinea-pigs. as a result of this inquiry it was found, that e. coli o115 27w and the dysentery-provoking serotype 185 are biological distinct bacteria which only share a minor cross relationship of their o-antigens, a ... | 1975 | 1106060 |
| localization of rate-limiting barrier to penetration of endotoxin through nonkeratinized oral mucosa in vitro. | the penetration of tritiated bacterial endotoxin through nonkeratinized oral mucosal epithelium was studied using an in vitro model system and radioautographic tracer techniques. the basement membrane region of the epithelium was the rate-limiting barrier to penetration, and this barrier effect was independent of the direction and duration of penetration as well as any clearance effects of the vasculature. | 1975 | 1104674 |
| the role of immune pig colostrum, serum and immunoglobulins igg, igm, and iga, in local intestinal immunity against enterotoxic strain in escherichia coli o55 in germfree piglets. | the protective effect of pig immune colostrum, serum and immunoglobulins igg, igm and iga against the enterotoxic strain of escherichia coli o55, was studied in newborn germfree piglets. this strain produced accumulation of fluid and dilatation of intestine when injected into the ligated ileal segment of germfree piglets, which is considered to be the typical effect of enterotoxins. erosion of the intestinal epithelium and penetration of bacteria into the submucosa were also observed. immune ser ... | 1975 | 1104425 |
| [course of beta-hemolytic e. coli carrier state in piglets during weaning]. | the investigations were carried out on 22 sows and 44 piglets (one sow and 2 piglets from each litter). the pigs were divided into 4 groups according to the different additions to their diet during weaning which might have an effect on the carrier state and dissemination of beta-haemolytic e. coli. group i was given a feed concentrate and separated milk, group ii - a feed concentrate and sour milk, group iii - a feed concentrate and milk with addition of garlic (200 mg/kg of body weight), group ... | 1975 | 1103097 |
| [the infectious (plasmidic) bacterial antibiotic resistance]. | 1975 | 1102479 | |
| leptospirae of serotype lora of the serogroup australis isolated for the first time from swine in the netherlands. | leptospirosis in swine, caused by serotype lora (serogroup australis) was detected both serologically and by culture. the most important symptoms consisted in abortion during the final month of gestation and the birth of dead or not viable piglets. | 1975 | 1101443 |
| [goblet cells in spontaneous colibacteriosis in pigs]. | this work is a part of the studies on the intestinal barrier undertaken to cast some more light on the pathogenesis of colibacteriosis in pigs. the objective of the work was to investigate the goblet cells in the intestines of pigs ill with spontaneous colibacteriosis in the form of oedema disease and gastrointestinal form. the investigations were carried out on 26 pigs segregated into 3 groups: i group -- typical oedema disease (12 pigs), ii group -- colibacteriosis in the gastrointestinal form ... | 1975 | 1101236 |
| relationships of intestinal enzymes and serum antitoxin to the pig response to escherichia coli enterotoxin. | the intestinal loop technique was used to evaluate the response of three week old piglets to the heat labile (lt) and the heat stable (st) enterotoxins produced by escherichia coli f11(p155). the serum anti-lt activity and the lipase, amylase and trypsin activities in the jejunal lumen of these pigs were determined. piglets responded independently ti each toxin and no relationship between these responses and serum anti-lt activity or the enzyme activities of the jejunal content could be demonstr ... | 1975 | 1100203 |
| factors involved in bactericidal activity of sheep serum. | the bactericidal activity of blood, plasma, and serum from sheep was measured with 3 strains of escherichia coli. plasma showed greater bactericidal activity than blood or serum for strains o78:k80(b) and lilly. the antibody-complement system of normal serum killed the rough strain, lilly, but neither antibody nor complement alone were bactericidal. antibody, complement, and an additional heat-stable cofactor were necessary for killing strain o78:k80(b). strain 3662 was not susceptible to the le ... | 1975 | 1098531 |
| in vitro study of the influence of virginiamycin and spiramycin on the composition and biochemical activities of the gastrointestinal flora of piglets. i. influence on the composition of the flora. | in vitro fermentations with the gastro-intestinal flora of 18 treated piglets (control, virginiamycin, spiramycin) were carried out to study the influence of nutritional doses of virginiamycin and spiramycin on the composition of the microflora. each total flora was used for three different incubations: control, 50 ppm virginiamycin, 50 ppm spiramycin. to obtain comparable results with experiments in vivo, an artificial fermentation medium was made on the basis of several chemical analyses of in ... | 1975 | 1098325 |
| a field study of coliform mastitis in sows. | three sows with agalactia and mastitis were compared with 3 normally lactating sows at 8, 16, 24, and 32 hours postpartum. bacteriologic examination of milk resulted in isolation of klebsiella pneumoniae or escherichia coli from affected glands of all 3 agalactic sows. milk from clinically normal glands of affected sows and from glands of normal sows was culture-negative for coliforms. affected sows had higher rectal temperatures and lower milk yields, and pigs from affected sows had lower mean ... | 1975 | 1097381 |
| heat-stable escherichia coli enterotoxin production in vivo. | hysterectomy-derived, colostrum-deprived piglets were infected with enterotoxigenic escherichia coli on day 4 of life. samples of feces and intestinal contents were collected and tested in infant mice for enterotoxic activity. positive enterotoxic responses were observed in mice given filtrates of feces and intestinal contents from piglets infected withe enterotoxigenic e. coli known to produce heat-stable enterotoxin but not heat-liabile enterotoxin in vitro. it is concluded that heat-stable en ... | 1975 | 1097335 |
| enteric colibacillosis: evaluation of strains of escherichia coli utilizing the ligated loop technique in gnotobiotic swine. | the enteropathogenicity of 6 strains of escherichia coli was evaluated via the ligated loop technique in 15 gnotobiotic swine 3 to 4 weeks old. strain enteropathogenicity in gnotobiotic swine, as determined on the basis of visual loop distention and light microscopic evaluation of intestinal sections, compared favorably with that reported in conventional swine, except that jejunal loops of gnotobiotic swine may be somewhat less sensitive to certain strains. in addition, postoperative mortality r ... | 1975 | 1096688 |
| [problems of resistance (author's transl)]. | the results of sensitivity tests performed during the period from 1970 up to 1973 inclusive are compared with those obtained in pervious years. this showed that resistance to penicillin had increased in staphylococci causing bovine mastitis. during each single year, however, there was a decrease. s. typhimurium isolated from cattle showed increased resistance to chloramphenicol, neomycin and ampicillin; s. dublin showed an increase in resistance to chloramphenicol. e. coli isolated from calves w ... | 1975 | 1096359 |
| the effects of feeding tetracycline, nitrovin and quindoxin on the drug-resistance of coli-aerogenes bacteria from calves and pigs. | 1975 | 1095540 | |
| relationships of antibiotics in animal feeds and salmonellosis in animals and man. | 1975 | 1095537 | |
| the in vitro and in vivo effects of carrageenin on humoral and cellular factors of natural resistance. | the inhibitory effect of carrageenin, a sulfated algal polygalactose, on humoral factors of natural resistance is discussed. in dependence on dose and time, the influence of non-specific and specific bacteriolysis, on the in vitro and in vivo opsonic activity and on the course of infection was studied. | 1975 | 1095463 |
| preweaning mortality in pigs. 4 diseases of the gastrointestinal tract in pigs. | the incidence of fatal gastroenteropathies in sucking pigs was studied during a 2-year period in 17 sow herds involving 2,936 litters. the results showed that 2.8 per cent of the liveborn pigs died with gastroenteropathies during the sucking and immediate post weaning period. the incidence was lowest in offspring from second-litter sows (table i) and the progeny from large litters tended to have the highest incidence (table ii). fatal gastrointestinal diseases were observed in a total of 17.6 pe ... | 1975 | 1094407 |
| some immunological aspects of liver function. | 1975 | 1093339 | |
| the sequence of amino acid residues around the oxidation-reduction active disulfide in yeast glutathione reductase. | a 14-residue peptide containing the oxidation-reduction active cystine residue from yeast glutathione reductase has been isolated from proteolytic digests of the enzyme in which the free sulfhydryl groups had been reacted with n-ethylmaleimide. the sequence of this disulfide-containing peptide was found to be:(see article). the sequence was highly homologous with the active cystine regions in escherichia coli and pig heart lipoamide dehydrogenase. the sequences of three of the postulated four th ... | 1975 | 1092678 |
| enzymatic arginylation of beta-melanocyte-stimulating hormone and of angiotensin ii. | porcine beta-melanocyte-stimulating hormone and angiotensin ii were examined as acceptors in the reaction catalyzed by arginyl-trna-protein transferase. both inhibited enzymatic transfer of [14c]arginine from trna to bovine albumin. inhibition was competitive with albumin and the k-i values were, respectively, 15 and 0.8 mum. the expected arginylated compounds were isolated and characterized. beta-melanocyte-stimulating hormone and its arginylated product had identical activities in the frog epi ... | 1975 | 1091639 |
| [bacteriocinogeny in yersinia enterocolitica]. | bacteriocinogeny was found in 19.8 per cent of y. enterocolitica strains tested. it was designated by the authors as enterocoliticacinogeny and the substance produced by the pathogen was named enterocoliticacine. bacteriocinogeny was found among the collection strains isolated in the south primorye and not among the strains isolated abroad. some cultures of y. enterocolitica isolated abroad and in the south primorye were used as indicator strains for revealing enterocoliticacinogeny. enterocolit ... | 1975 | 1091204 |
| escherichia coli infections in piglets: pathogenesis, virulence and vaccination. | 1975 | 1091085 | |
| [pathogenesis of toxin caused gastroenteritis of vibrios and coli bacteria]. | 1975 | 1089519 | |
| the effect of antisera on porcine enteropathogenic escherichia coli in ligated segments of pig intestine. | nineteen antisera produced in pigs against 14 enteropathogenic and five nonenterotoxigenic porcine strains of escherichia coli were tested for their ability to inhibit gut loop fluid accumulation induced by homologous and heterologous organisms. in addition, four antisera produced in pigs by an intensive series of intravenous inoculations and three by a less intensive series of intramuscular injections of a polyvalent e. coli vaccine were evaluated. antisera were also produced in rabbits against ... | 1975 | 1089462 |
| [the effect of furazolidone or carbadox in the starter mixture for early-weaned piglets]. | for a period of 14 days, piglets from six litters, weaned between the 25th and 28th day of age, were fed the cos 2 starter containing either a premix with furazolidone or carboadox of czechoslovak origin. bentonite hydrosilicate was used as a carrier in both cases. furazolidone administered in the dose of 200 mg per 1 kg of feed prevented diarrhoea, insignificantly increased body weight gain, and decreased the consumption of feed per 1 kg of gain from 5.6 kg in the control to 4.0 kg in the test ... | 1976 | 828986 |
| interference by neisseria gonorrhoeae growth by other bacterial species. | growth of neisseria gonorrhoeae from clinical specimens has been enhanced by the use of selective media that inhibit the simultaneous growth of other microorganisms. one explanation for this enhancement could be that certain other bacteria inhibit gonococcal growth. this hypothesis was examined by testing 167 bacterial isolates for in vitro gonococcal inhibition; 34.1% of the isolates failed to inhibit the gonococcus, but 12.0% produced weak inhibition and 53.9% strongly inhibited n. gonorrhoeae ... | 1976 | 823175 |
| the in vivo opsonizing activity of fab mu and f(c)5 mu fragments of pig immunoglobulin m to escherichia coli 0 55 in newborn, precolostral piglets. | 1975 | 812799 | |
| [false-positive results obtained on examining slaughtered animals for the presence of antibiotic residues (author's transl)]. | as part of the examination of emergency-slaughtered animals for the presence of antibiotic residues, studies were done to see whether false-positive results would be obtained when the sarcina lutea kidney test and bacillus subtilis bga test were performed. when the s. lutea kidney test was positive in cattle, calves and swine, penicillin was invariably found to be present in those animals, the histories of which showed that they had not been given antibiotics. a syringe and an injected fluid con ... | 1975 | 809859 |
| in vitro study of the influence of virginiamycin and spiramycin on the composition and biochemical activities of the gastrointestinal flora of piglets. ii. influence on the biochemical activities of the microflora. | chemical analysis was carried out on 54 in vitro fermentations to study the influence of nutritional doses of virginiamycin and spiramycin on the metabolic activity of the gastro-intestinal flora of 18 in vivo treated piglets. changes in the decomposition of glucose and the production of nh3, volatile acids and lactic acid are recorded in function of time. these results are in good accordance with changes noted in the composition of the bacterial flora, such as a marked decrease in the productio ... | 1975 | 808048 |
| [actinomycin do inhibition of rna biosynthesis and its interaction with dna preparations]. | actinomycin d0 differing from actinomycin d in replacement of i residue of sarcosine by glycine in one of the peptide chains was found to have a lower inhibitory effect on rna synthesis in various biological systems. on interaction of e. coli with dna actinomycin d0 formed a complex with dna having the binding constant k=1.6 with 10-5 which was 100 times lower than the binding constant of actinomycin d. therefore, actinomycin d0 formed a complex with dna which was 100 times less stable than acti ... | 1975 | 806255 |
| [effect of acetylsalicylic acid on experimentally induced endotoxin reactions in swine]. | the time and severity of clinical responses to endotoxin depend on dosage, administration of acetylsalicyclic acid and indometacin prior to endotoxin application may delay or moderate the clinical phenomena that are likely to result from certain endotoxin amounts. | 1976 | 798559 |
| [experimental studies on the pathogenesis of coli enterotoxemia in swine. 3. effect of coli enterotoxin in weaned piglets following intragastric administration]. | the following results were obtained from experiments with 23 clinically intact weaned piglets, aged between five and six weeks (18 animals) or eight weeks (five animals), which had received three intragastric applications of crude enterotoxin (culture filtrate supernatant of escherichia coli o149:k91(b)k88(l):. 1. twelve animals (52 per cent) responded to enterotoxin application by temporary outbreaks of diarrhoea (1-8.5 hours) following differentiated latencies (2.5-10 hours). no diarrhoea was ... | 1976 | 798558 |
| [drug resistance and virulence of citrobacter isolated from the focus of piglet intoxication]. | 1976 | 797596 | |
| the mutagenicity of cyclamates and their metabolites. | 1976 | 796692 | |
| characterization of enterotoxigenic bovine escherichia coli. | among 300 isolates of bovine escherichia coli, 56 which had been found enterotoxigenic in calf gut loops were characterized on the basis of o and k antigens, colonial morphology and resistance to seven antimicrobial drugs. the 56 isolates enterotoxigenic in the calf were compared with the nonenterotoxigenic ones. of the 56 enterotoxigenic e. coli the majority possessed the a type of k antigen and had ok groups, o9:k(ps274) or o101:k(rvc118). fourteen of these isolates had the k99 antigen. none o ... | 1976 | 793694 |
| escherichia coli strains isolated from diarrheic piglets in the province of quebec. | during 1972 to 1974, 112 escherichia coli strains isolated from diarrheic piglets were recieved from different parts of the province of quebec, canada. fifty-six strains elicited a positive gut loop response in three week old piglets and were then considered as moon's class 1 enteropathogens, while four of the 56 remaining strains reacted only in ten day old piglets and were classified as class 2 enteropathogens. forty-eight strains produced both a heat-labile and a heat-stable enterotoxin, whil ... | 1976 | 793691 |
| [chemotherapeutic efficiency of olaquindex in experimental e.coli-diarrhea and enterotoxaemia of piglets]. | 1976 | 793001 | |
| [change in the virulence of flexner shigellae following transmission of several parts of chromosomes related to the synthesis of o-antigen lipopolysaccharide from e coli k-12 and r-mutants of shigellae]. | 1976 | 790821 | |
| etiologic diagnosis of diarrheal disease of calves: frequency and methods for detecting enterotoxin and k99 antigen production by escherichia cola. | escherichia coli isolated from calves in minnesota and montana were tested for enterotoxigenicity via bio-assay of cell-free broth culture fluid and for k99 antigen via a serum agglutination test. infant mice were used to assay for heat-stable enterotoxin (st), and adrenal cells in culture were used to assay for heat-labile enterotoxin (lt). forty-six of the 345 e coli isolates produced st enterotoxin, but none produced lt enterotoxin. thirty-five of the 46 enterotoxigenic isolates had k99 antig ... | 1976 | 786085 |
| [the role of environmental pollution in the etiology of endemic nephropathy]. | 1975 | 785634 | |
| proceedings: polypeptide chain elongation in bacterial and mammalian systems. | 1976 | 776955 | |
| [e. coli enterotoxicosis in unweaned piglets. iv. evaluation of results obtained on using an adjuvant vaccine in the field (author's transl)]. | the results obtained on using an adjuvant vaccine against enterotoxaemia caused by e. coli in suckling piglets are discussed on the basis of an inquiry conducted among a number of veterinary practitioners. the vaccine contains the ok groups o138:k81,k88, o141:k85ab and o149:k91,k88 and is administered parenterally to the dams during the latter half of gestation. the inquiry which covered 4,000 sows on fifty-five farms showed that, as regards diarrhoea during the first week of life, morbidity in ... | 1976 | 772879 |
| [e. coli enterotoxicosis in unweaned piglets. iii. preparation and use of vaccines in enterotoxaemia due to e. coli in newborn piglets in the netherlands]. | the preparation of a number of e. coli vaccines designed to prevent enterotoxicosis due to e. coli in young piglets is discussed. the results of laboratory experiments concerned with the production of haemagglutinating antibodies in pigs following inoculation of this vaccine are reported. this oil-adjuvant vaccine (oav) was used in the field in thirty-one herds in which enterotoxicosis due to e. coli was a recurrent problem. it is concluded from serological and clinical observations that the use ... | 1976 | 772878 |
| [e. coli enterotoxicosis in unweaned piglets. i. some concepts (author's transl)]. | in a brief review, attention is drawn to a number of findings on infections with enteropathogenic e. coli in suckling piglets: serological typinge k88 antigen, colonisation, enterotoxin production and enterosorption, absence of symptoms of inflammation in the bowel wall and significance of the various immunoglobulin classes. the term e. coli enterotoxicosis used to designate the disease is preferred to terms such as e. coli diarrhoea, e. coli enteritis and (enterotoxic) colibacillosis. | 1976 | 772876 |
| the pathogenesis of reflux nephropathy (chronic atrophic pyelonephritis). | the relationship between vesico-ureteric reflux and coarse renal scarring (atrophic pyelonephritis) has been studied in swine. scars were observed to develop where reflux took place into the kidney substance via the renal papillae (intrarenal reflux). they were confined to these regions and were similar in size, distribution and other features peculiar to those found in the human from early childhood onwards. intrarenal reflux was found to be related to the pressure within the urinary tract as ... | 1975 | 766885 |
| [changes in the virulence of colibacteria under the influence of various biological factors]. | 1975 | 766580 | |
| [study of the role of colicins in the formation of microbial association in experiments in chick embryos]. | experiments were conducted on chick embryos; conditions of formation of bacterial cenosis of shigella sonnei and e. coli were reproduced. the role of colicinogenia in the formation of bacterial cenosis was demonstrated. an accumulation of colicines in high titres (1: 5120- 1:2560) in the chick embryonic cavity was noted in combined or separate cultivation of colicinogenic shigellae and e. coli. an intergenera transmission of the col-factor was traced in experiments on chick embryos. the data obt ... | 1975 | 766520 |
| [residues of antimicrobial materials in the effluents of livestock farms (author's transl)]. | forty water samples were taken from streams of three different regions of belgium, with intensive husbandry and examined for residual microbiological activity. in some samples, activity was detected against staphylococcus aureus; none of them were active against e. coli and c. albicans. the substances in the few positive samples were not identified. with this low activity and the small proportion of positive samples (12 out of 41) any influence on the water biosphere due to the methods of farmin ... | 1976 | 766304 |
| acrolein. | 1978 | 415230 | |
| septic shock in obstetrics and gynecology. pathophysiology. | 1977 | 407404 | |
| [activity of various enzymes in the blood, liver, intestines and kidneys in spontaneous colibacteriosis of piglets]. | the purpose of the studies was to determine the activity of enzymes in the serum and pig organs with colibacteriosis in the form of oedema and stomach-intestines disease playing a diagnostic role in the determination of the organ damage degree. studies were carried out on 29 pigs. the activity of lap, gpt, got, am, fdpa, lp, ap and acp was determined in the serum liver, empty intestines and kidneys of the diseased and control pigs. it resulted from the experiments that in the serum of all the di ... | 1979 | 400185 |
| enterotoxins of enteric bacteria--a review. | a review of the enterotoxins of enteric bacteria was compiled, taking into account recent developments of the field as well as results of the author's own research work. after characterization of enterotoxins of different origin, their pathogenic role is described, with special attention to the genetic background of these agents. the possible use of enterotoxins for active immunization purposes is also discussed. | 1979 | 399581 |
| course of antibiotic sensitivities in escherichia coli and staphylococcus aureus from animals. | in order to establish the evolution of resistance against the common antibiotics from the beginning of their use up to the present time, the sensitivity of escherichia coli isolated from the cecum of wild boars was determined. the mic's of e. coli from these animals in a zoological garden was examined for comparison. the samples from the wild boars were collected in five forests in the belgian ardennes. the mic's obtained (table 1) are comparable to the mean values given in the early literature ... | 1979 | 397700 |
| some biological characteristics of escherichia coli strains isolated from acute diarrhoeal diseases of infants. | the aim of this study was to evaluate the methods of biological characterization of escherichia coli strains, in order to use them as screening tests in clinical microbiological laboratories. in two thirds of the 45 e. coli, isolated from acute intestinal infection of infants, different factors of virulence were found. the strains showed prevalently an enterotoxigenic character (66,6%), which was in correlation with the increased permeability (62,2%). adhesive properties were present in 20% of e ... | 1979 | 396738 |
| [immunobiological activity of various blood serum fractions from cattle and swine. the dynamics of ig in specific serum against colibacillosis in pigs]. | the level of the igm and igg agglutinating antibody specific hyperimmune serum against coli-bacteriosis and its fractions were studied. the serum was obtained at various stages of the preparation, hyperimmunization and exploitation of serum producing swine by using the 2-mercapto-ethanol test. it was established that serum of the total globulin, alpha-beta-globulin and gamma-globulin fractions contains igm and igg agglutinating antibodies against e. coli. these immunoglobulin classes are synthet ... | 1979 | 396718 |
| duration of anti-adhesive and bactericidal activities of milk from vaccinated sows on escherichia coli o149 in the digestive tract of piglets during the nursing period. | piglets were exposed orogastrically to escherichia coli to enable study of the duration of anti-adhesive and bactericidal activities of milk of sows vaccinated with a k88 enriched e coli vaccine. there was a marked increase in the number of the challenge strain in the digestive tract of weaned piglets of all ages (between 888 and 2144 per cent). in contrast, there was a decrease in their number (75 per cent) in the day-old colostrum-fed piglets. when the piglets were two weeks old milk was still ... | 1979 | 396618 |
| [causative agents of category-ii escherichiasis and the results of studies testifying to their circulation in the ussr]. | 1978 | 394394 |