Publications

[bacteremia caused by vibrio cholerae no. 01].		1990	2355781
further studies on the gangliosidic nature of the cholinergic-specific antigen, chol-1.	the antigen designated as chol-1 beta, detected by an antiserum specific for cholinergic neurons, has been purified to homogeneity from ganglioside mixtures extracted from torpedo electric organ and pig brain. the final products from the two sources behaved identically in a wide range of tests and gave coincident immunopositive and ehrlich-positive spots after thin layer chromatography in seven different solvent systems; they were thus considered to be identical and to constitute a single, pure ...	1990	2353821
lipid a mutants of vibrio cholerae: isolation and partial characterization.	vibrio cholerae mutants resistant to common antibiotics and neutral and anionic detergents were isolated. analysis of isolated outer membranes revealed a significant deficiency in the acylation of lipid a in the resistant strains. the content of amide-linked and ester-bound fatty acids in the lipid a of the mutant strains compared to that of the wild type was about 50-56% and 29-37% respectively. this defect was specific for lipid a as there was no change in the acylation of phospholipids. the r ...	1990	2350336
the effect of endotoxin on membrane fatty acid composition in bcg-sensitized mice.	the effects of endotoxin on mouse liver phospholipid fatty acid composition have been investigated. administration of endotoxin from salmonella abortus equi led to a decrease in the polyunsaturated fatty acid content of livers from mice sensitized with bacille calmette guérin (bcg). the content of arachidonic acid fell significantly in both the phosphatidylcholine and phosphatidylinositol fractions whereas in the phosphatidylethanolamine fraction the linoleic acid content was significantly reduc ...	1990	2347397
bismuth subsalicylate in the prevention of colonization of infant mice with campylobacter jejuni.	infant mice were used for the evaluation of the efficacy of bismuth subsalicylate (bss) in the prevention of the growth of campylobacter jejuni in the intestine. the mic90 of ten c. jejuni strains was 900 micrograms/ml. of three dosage regimens tested, continuous treatment before and after the bacterial challenge, mimicking the way bss is used in the prevention of traveller's diarrhoea, was the most effective. growth inhibition was dose dependent; the high dose of 2000 micrograms per day was mor ...	1990	2347383
actin gene expression in murine erythroleukemia cells treated with cytochalasin d.	the expression of cytoskeletal protein genes may be linked to both cell growth and the status of the cytoskeleton. actin gene expression was examined in murine erythroleukemia cells treated with the microfilament disrupting agent, cytochalasin d (cd), at a concentration which was determined to inhibit cell growth and arrest cells in the s and g1 phase of the cell cycle. levels of actin mrna and protein synthesis were elevated eight- and sixfold, respectively, after 9 h in cd. this increase was r ...	2008	2347376
identity of pb76 differentiation antigen and lymphocyte alkaline phosphatase.	alkaline phosphatases (apases, ec 3.1.3.1) are ecto-enzymes bound to cell membranes by a phosphatidyl-inositol anchor. we have previously shown that apase is present on activated murine b cells and its expression correlates with the process of b cell differentiation into immunoglobulin secretion. recently, a monoclonal antibody (mab), g-5-2, that recognizes a 76-kda molecule preferentially expressed on the surface of pre-b and plasma cells (pb76) was described. some features shared by apase and ...	2008	2347370
phase-transfer-catalyzed synthesis of aryl alpha-ketosides of n-acetylneuraminic acid. a 2-methylfluoran-6-yl glycoside of n-acetylneuraminic acid, 2-methyl-6-(5-acetamido-3,5-dideoxy-alpha-d-glycero-d-galacto- nonulopyranosylonic acid)xanthene-9-spiro-1'-isobenzofuran-3'-one, a new substrate for neuraminidase assay.	glycosidation of n-acetylneuraminic acid by phase-transfer catalysis in chloroform-aqueous alkali gave several known and some new aryl alpha-ketosides in a short reaction time and in good yields. the 4-methylumbelliferyl alpha-ketoside, the standard substrate for neuraminidase, was prepared in a yield of up to 70%. new neu5ac ketosides were prepared with fluorescein and the fluorescein analog, 2-methyl-6-hydroxyfluoran (2-methyl-6-hydroxyxanthene-9-spiro-1'-isobenzofuran-3'-one) as aglycons, the ...	1990	2346942
a model for teaching periodontal surgery.	a simple laboratory-made model is described for periodontal surgical technique exercises. it has proved useful for tutor demonstration and student practice of all aspects of flap management.	2008	2346697
'an unusual hazard of toothbrushing'.		2008	2346693
inhibitory effect of calcium-binding protein regucalcin on protein kinase c activity in rat liver cytosol.	regucalcin, a calcium-binding protein isolated from rat liver cytosol, inhibited ca2(+)- and phospholipid-dependent protein kinase (protein kinase c) activity in hepatic cytosol. with the increasing concentrations of ca2+ or phosphatidylserine in the medium, regucalcin caused a remarkable inhibition of protein kinase c activity. moreover, regucalcin significantly inhibited dioctanoylglycerol-activated protein kinase c. regucalcin itself did not have protein kinase activity in either the presence ...	1990	2346670
transferable trimethoprim resistance of vibrio cholerae o1 encountered in southern india.		1990	2345916
cholera epidemic in solapur during july-august, 1988.	of the 77 vibrio cholerae isolated during july to september, 1988, 76 were el tor vibrios serotype ogawa, 68 belonging to t2 phage (eight strains untypable). peak incidence was noted in the month of august, 1988. haemolytic and non-haemolytic colony variants of el tor v. cholerae were encountered. all strains showed resistance to one or more antibiotics. no fatality was reported during the epidemic. the epidemiological data collected over the past three years (1986-88) show that cholera is preva ...	1990	2345026
physical demonstration of a high molecular weight bacteriocin plasmid in vibrio cholerae by genetic transformation process.	bacteriocinogeny was transferred at high frequencies from bacteriocinogenic (bac+) v. cholerae strains to non-bacteriocinogenic (bac-) recipients in the in situ genetic transformation system on agar surface. dna extracted from samples of growth of bacteria transformed to bac+ were obtained at 2 h intervals following contact with the sterile agar surface where the donor had grown previously. this showed acquisition of a high molecular weight plasmid which could be physically demonstrated best in ...	1990	2345019
[inheritance and expression of cholera toxin genes introduced into vibrio cholerae el tor cells in a hybrid plasmid].	two replicons, pox38 (in f-factor derivative lacking all is elements) and pct105 (containing cholera toxin operon cloned in pbr322) have been fused to produce recombinant plasmid, pco109, which was then introduced into vibrio cholerae eltor by conjugation. restriction analysis showed pco109 to dissociate in v. cholerae cells at a higher frequency than in escherichia coli strains, its pox38 component being lost, while the pct105 component demonstrated relative stability. v. cholerae eltor rv79 (p ...	1990	2344953
fatalities associated with vibrio parahaemolyticus and vibrio cholerae non-o1 infections in florida (1981 to 1988).	vibrio infections constitute a continuing source of morbidity and mortality in florida. seven fatal infections caused by vibrio parahaemolyticus or v cholerae non-o1 were reported in florida between 1981 and 1988. review of those seven medical records and vibrio case investigation forms showed that although all patients died of sepsis, gastrointestinal signs and symptoms characterized the early illness in four patients, whereas the other three initially had painful swelling and/or lesions of the ...	1990	2343327
immunobiological relationships of the enterotoxins produced by cholera toxin gene-positive (ct+) and -negative (ct-) strains of vibrio cholerae o1.	the optimum rabbit ileal loop (ril) reacting doses of the new cholera toxin (nct) produced by cholera toxin gene-negative (ct-) strain x-392 and of the enterotoxin produced by cholera toxin gene-positive (ct+) strain 569b of vibrio cholerae o1 were found to be 32 micrograms and 22 micrograms respectively. production of nct by the ct+ strain, in addition to ct, was confirmed by in-vivo neutralisation tests. anti-569b-enterotoxin neutralised the optimum ril reacting activity of nct completely at 1 ...	1990	2342085
person-to-person transmission of cholera in a psychiatric hospital.	an outbreak of cholera caused by vibrio cholerae o1, biotype el tor, serotype ogawa, phage type 4, was reported in a psychiatric hospital in singapore. a total of 74 inmates (18 symptomatic and 56 asymptomatic) were infected; two of them died. extensive epidemiological investigations showed that the organism was not transmitted by contaminated food or water but through close person-to-person contact. early recognition of the outbreak and prompt implementation of epidemic control measures compris ...	1990	2341728
contrast dependence of short-range apparent motion.	the apparent motion of band-pass filtered random dot kinematograms was assessed by measurements of two alternative direction discrimination performance. in the case of two-dimensional (isotropically filtered) stimuli, the results were largely independent of contrast: at michelson contrasts of 5 and 50%, near-perfect direction discrimination was obtainable for a limited range of displacements. however for one-dimensional (grating) stimuli, apparent motion seems to be highly dependent on contrast. ...	2008	2336804
nucleotide sequence of the gene, ompw, encoding a 22kda immunogenic outer membrane protein of vibrio cholerae.		1990	2336399
biotype-specific probe for vibrio cholerae serogroup o1.	the o1 serogroup of vibrio cholerae can be divided into two biotypes, el tor and classical. current tests to distinguish between these biotypes are often difficult to interpret. on the basis of the difference in sequence of the hlya gene in these biotypes, we have developed a simple probe that can easily and reliably differentiate between the two biotypes.	1990	2332478
effect of iron on production of a possible virulence factor by plesiomonas shigelloides.	plesiomonas shigelloides, when grown in an iron-poor medium (syncase), produces a substances that causes elongation of chinese hamster ovary (cho) cells similar to that produced by cholera toxin. when syncase is supplemented with iron, the ability of p. shigelloides (but not of vibrio cholerae) to produce this elongation of cho cells is lost. iron depletion of the growth medium appears to be essential for the cho cell elongation produced by p. shigelloides but is not essential for the production ...	1990	2332475
analysis of the 2-keto-3-deoxyoctonate (kdo) region of lipopolysaccharides isolated from non-01 vibrio cholerae 05r.	phosphorylated 2-keto-3-deoxyoctonate (kdo) has been detected in the strong-acid hydrolysates of lipopolysaccharides (lps) of family vibrionaceae including vibrio cholerae. structural analysis of lps isolated from a rough mutant of non-01 v. cholerae 05 by dephosphorylation, periodate oxidation and methylation analysis revealed that the inner core region of the lps molecule contains only one mole of kdo in contrast to enteric gram-negative bacterial lps, and that the phosphate group on the kdo m ...	1990	2332154
effects of dnase production, plasmid size, and restriction barriers on transformation of vibrio cholerae by electroporation and osmotic shock.	attempts to transform wild type strains of v. cholerae with plasmid dna by traditional osmotic shock methods were not successful. a mutant of v. cholerae that was deficient in extracellular dnase was transformed with plasmid dna by osmotic shock, demonstrating directly that extracellular dnase is a major barrier to transformation of v. cholerae. transformation of wild type and dnase-negative strains of v. cholerae was accomplished by electroporation. efficiency of transformation by electroporati ...	1990	2332153
[the toxin-producing capacity of populations of vibrio cholerae of different origins].	as the result of the study of toxin formation in 165 v. eltor strains having different virulence, most of the virulent cultures have shown stable toxin production, though 5-10% of their colonies have proved to be nontoxigenic. in faintly virulent strains toxin production was found to be unstable, which is indicative of the heterogeneity of their population composition as regards their capacity for toxin formation. the population of avirulent strains consists mainly of nontoxigenic clones (95.7%) ...	1990	2330784
evaluation of children with possible appendicitis using technetium 99m leukocyte scan.	we evaluated the use of technetium 99m albumin colloid white blood cell (tac-wbc) scan in 33 children with possible appendicitis. ten children had appendicitis, four of whom had perforated their appendix at the time of surgery. children with appendicitis differed from children without appendicitis in the incidence of right lower quadrant rebound tenderness (60% vs 17.4%), and white blood cell count (x10(3)/mm3) (14.0 + 1.9 vs 10.7 +/- 1.0). tac-wbc scans were interpreted as either positive, nega ...	2008	2330248
continuous hemodiafiltration in children.	continuous arteriovenous hemofiltration is a form of renal replacement therapy whereby small molecular weight solutes and water are removed from the blood via convection, alleviating fluid overload and, to a degree, azotemia. it has been used in many adults and several children. however, in patients with multisystem organ dysfunction and acute renal failure, continuous arteriovenous hemofiltration alone may not be sufficient for control of azotemia; intermittent hemodialysis or peritoneal dialys ...	2008	2330246
nurses' use of first names.		2008	2330156
abortion. ru486 under attack.		2008	2330054
[flexible arrangement of working time in the field of nursing--a contribution to optimal care].		2008	2329782
[hemophagocytic syndrome in two elderly men].	case 1: a 68-year-old male, who was admitted to our hospital because of massive intestinal bleeding, died 1 day after admission. case 2: an 80-year-old male, who was admitted to our hospital because of a loss of consciousness, died 4 days after admission. in both cases prodromic infection was not detected. at autopsy, phagocytosis of rbc and wbc by histiocytes was shown markedly in the spleen in case 1 and in the bone marrow in case 2. so diagnosis of virus-associated hemophagocytic syndrome was ...	2008	2329691
contempo '90.		2008	2329645
lipopolysaccharides of non-cholera vibrios possessing common antigen factor to 01 vibrio cholerae.		1990	2327294
[treatment of acute chloroquine poisoning].	acute chloroquine poisoning is life threatening with risk of death from apnoea and cardiac arrhythmia within a few hours of ingestion. mechanical ventilation, infusion of pressor agents and large doses of diazepam seem to provide effective treatment. this treatment was introduced by riou et alii (n engl j med 1988; 318; 1-6), and we used it successfully in a case of severe chloroquine poisoning. intensive treatment was given during the first two days of intoxication, when the whole blood chloroq ...	2000	2327037
a comparison of pediatric versus adult cadaver donor kidneys for transplantation.		1999	2326916
successful treatment of hepatic venoocclusive disease in a bone marrow transplant patient with orthotopic liver transplantation.		1999	2326879
[family work: cooperation with families of young schizophrenics--a new dimension in psychiatric nursing].		1999	2326744
vibrio cholerae bacteremia associated with gastrectomy.	bacteremia due to vibrio cholerae is rare. each of 15 cases previously reported in the english language literature occurred in the setting of immune deficiency. we describe an instance of non-serogroup o1 v. cholerae septicemia in an otherwise healthy patient. susceptibility to such infection may have been enhanced by a prior gastrectomy for duodenal ulcer.	1990	2324281
novel antibiotics, furaquinocins a and b. taxonomy, fermentation, isolation and physico-chemical and biological characteristics.	two novel antibiotics, furaquinocins a and b were isolated from the culture broth of streptomyces sp. ko-3988. these antibiotics possess cytocidal activities against hela s3 cells in vitro at concentrations of 3.1 micrograms/ml for a and 1.6 micrograms/ml for b. neither substance possessed antimicrobial activities against gram-positive and gram-negative bacteria, fungi or yeast at a concentration of 1,000 micrograms/ml.	1990	2324009
long-term clearance of inhaled uo2 particles from the pulmonary region of the rat.	this paper reports on an enriched uranium dioxide (uo2) mass clearance study undertaken with fischer-344 rats. the uo2 had a uranium (u) isotopic composition of 0.79% 234u, 92.8% 235u, 0.34% 236u and 6.06% 238u, by mass, with an alpha-particle activity of 1.91 bq micrograms-1. forty-six rats were exposed to an enriched uo2 aerosol that had an activity median particle aerodynamic diameter ranging from 2.7 to 3.2 microns. the rats were killed from 1 to 720 d post-inhalation (pi). the mass of enric ...	1990	2323929
diminished immunogenicity of a recombination-deficient derivative of vibrio cholerae vaccine strain cvd103.	to address potential concerns over the release of genetically engineered live bacterial vaccines, we constructed a recombination-deficient derivative of the vibrio cholerae o1 vaccine strain cvd103 (cvd103rm). oral immunization of adult volunteers with cvd103rm showed that the reca mutation significantly diminished colonization ability and immunogenicity of the vaccine strain.	1990	2323828
production, characterization, and species specificity of monoclonal antibodies to mycobacterium avium complex protein antigens.	the incidence of mycobacterium avium-mycobacterium intracellulare complex infections has increased in recent years primarily because a significant proportion of acquired immunodeficiency syndrome patients develop disseminated m. avium complex disease. in an effort to develop new tools to study these infections, we have produced eight monoclonal antibodies directed against m. avium. western blot (immunoblot) specificity analysis and protease sensitivity assays indicate that four of these antibodi ...	1990	2323822
comparative analysis of the hemolysin genes of vibrio cholerae non-01, v. mimicus, and v. hollisae that are similar to the tdh gene of v. parahaemolyticus.	the genes encoding the hemolysins similar to the thermostable direct hemolysin (tdh gene) of vibrio parahaemolyticus were cloned from chromosomes of v. mimicus and v. hollisae. these cloned hemolysin genes and previously cloned tdh genes of v. parahaemolyticus and v. cholerae non-01 were compared by physical mapping and by hybridization with oligodeoxyribonucleotide probes. the nucleotide sequences in the coding regions of all the cloned hemolysin genes were very homologous and had only minor va ...	1990	2323548
modulation of tartrate-resistant acid phosphatase expression by calcitriol in csf-induced macrophage colonies.	the osteoclast is thought to be a hemopoietically derived cell, but questions exist about which hemopoietic growth factors are responsible for proliferation of osteoclast precursors. experiments were thus performed to see if recombinant human colony-stimulating factors (csfs) influenced the expression of tartrate-resistant acid phosphatase (trap), an osteoclast marker enzyme, by monkey bone marrow colonies in vitro. in addition, the effect of 1,25-dihydroxyvitamin d3 (calcitriol) on csf-induced ...	1990	2323362
contact sensitization to diazolidinyl urea: report of 3 cases.		2008	2323203
epidemic cholera in west africa: the role of food handling and high-risk foods.	during an epidemic of cholera in guinea, west africa, in 1986, the authors conducted two studies of risk factors for transmission. in the capital city, 35 hospitalized cholera patients were more likely than 70 neighborhood-matched controls to have eaten leftover peanut sauces (odds ration (or) = 3.1, 95% confidence interval (ci) 1.2-8.2), but less likely to have eaten tomato sauces (or = 0.2, 95 percent ci 0.1-0.9). hand washing with soap before meals by all family members protected against chol ...	1990	2316500
experimental non-o group 1 vibrio cholerae gastroenteritis in humans.	in this study, 27 volunteers received one of three non-o group 1 vibrio cholerae strains in doses as high as 10(9) cfu. only one strain (strain c) caused diarrhea: this strain was able to colonize the gastrointestinal tract, and produced a heat-stable enterotoxin (nag-st). diarrhea was not seen with a strain (strain a) that colonized the intestine but did not produce nag-st, nor with a strain (strain b) that produced nag-st but did not colonize. persons receiving strain c had diarrhea and abdomi ...	1990	2312721
digestibility of cotton plant by-product diets for sheep at two levels of intake.	crossbred wethers (n = 48) were assigned randomly to 1 of 12 experimental diets, arranged in a 3 x 2 x 2 split plot to evaluate three levels of cotton plant by-product (25, 50 and 75% cpb), two levels of naoh treatment of cpb (0 and 4%) and two levels of monensin supplementation (0 and 30 ppm, dm basis) at two intake levels (70 and 90% of ad libitum). intake was a within-lamb variable. ad libitum intake was established over an initial 17-d adjustment period. feed intake and fecal output were rec ...	1990	2312420
behavioral problems in old dogs: 26 cases (1984-1987).	among 26 dogs greater than or equal to 10 years old, the most frequent owner complaints relating to behavioral problems were destructive behavior in the house (n = 10), inappropriate urination or defecation in the house (n = 10), and excessive vocalization (n = 7). the most frequent behavioral diagnoses were separation anxiety (n = 13) and breakdown of housetraining (n = 6). most of the behavioral problems in the 26 dogs began after the dogs reached the age of 10 years, and most of the dogs had ...	1990	2312394
antisecretory and antiinflammatory properties of bismuth subsalicylate.	the antisecretory properties of pepto-bismol (pb) and its active ingredient, bismuth subsalicylate (bss), were studied in ligated (rabbit and pig) and unligated (rat) intestinal-segment models. when pb was administered to segments before intestinal inoculation with heat-labile (lt) escherichia coli or vibrio cholerae enterotoxins, the inhibition of fluid accumulation was 74%-94% and 60%-91%, respectively. in the pig, the percentages of inhibition by pb of fluid accumulation produced by organisms ...	1990	2305173
in vitro activity of norfloxacin and other antibacterial agents against gastro-intestinal pathogens isolated in sweden.	the in vitro activity of norfloxacin was compared to that of ampicillin, doxycycline, chloramphenicol, trimethoprim in combination with sulfamethoxazole (1/20), and erythromycin, against 272 clinical isolates of gastro-intestinal pathogens. norfloxacin was the most active compound of those tested with mics in the range 0.004-2 mg/l. concentrations inhibiting 90% of the strains (mic 90) were 0.008 mg/l for vibrio cholerae, 0.016 mg/l for aeromonas hydrophila, 0.032 mg/l for vibrio cholerae non 01 ...	1990	2302351
vibrio cholerae bacteremia in a 22-month-old new mexican child.		1990	2300417
isolation of vibrio cholerae non-01 from a somerset farmworker and his tropical fish tank.	vibrio cholerae non-01 is occasionally isolated from wounds. to our knowledge, this is the first such case to be reported from the u.k. it was associated with isolation of a similar organism from a tropical fish tank.	1990	2299183
comparison of a latex agglutination assay and an enzyme-linked immunosorbent assay for detecting cholera toxin.	to determine the pandemic potential of vibrio cholerae, one must demonstrate both the presence of o1 antigen and the production of enterotoxin (ct). tissue culture or enzyme-linked immunosorbent assays (elisas) for ct have been limited to research and reference laboratories. a kit for detecting ct by reversed passive latex agglutination is now commercially available and was used to test 168 strains of v. cholerae o1 and non-o1. when compared with the routine elisa, the latex test was 98% accurat ...	1990	2298870
investigation by amperometric methods of intracellular reduction of 2,6-dichlorophenolindophenol in normal and transformed hepatocytes in the presence of different inhibitors of cellular metabolism.	the reduction of 2,6-dichlorophenolindophenol (dcip) was measured by amperometric methods in morris hepatoma 3924a cells, normal isolated rat hepatocytes and in mitochondria isolated from normal rat liver. the influence of aerobic and anaerobic atmospheres and of various inhibitors of cellular metabolism, especially of the respiratory chain (kcn, nan3, oligomycin), on dcip-reduction were studied using glucose, succinate, beta-hydroxybutyrate, alpha-keto-glutarate and oxalacetate as substrates. u ...	1990	2297512
[hemoglobin, serum iron and transferrin saturation among users of intrauterine devices and oral contraceptive agents].	we studied 60 females using either intrauterine device or taking oral contraceptive pills. hemoglobin, serum iron, total iron binding capacity and saturation of transferrin were determined before and 4 and 10 months after starting a responsible paternity program. women with a basal hemoglobin level below 12 g/dl were excluded. age, parity and hematologic parameters were similar for both groups. a significant decrease in hemoglobin level and saturation of transferrin was observed at 10 months in ...	2007	2293270
[hemoglobin, serum iron and transferrin saturation among users of intrauterine devices and oral contraceptive agents].	we studied 60 females using either intrauterine device or taking oral contraceptive pills. hemoglobin, serum iron, total iron binding capacity and saturation of transferrin were determined before and 4 and 10 months after starting a responsible paternity program. women with a basal hemoglobin level below 12 g/dl were excluded. age, parity and hematologic parameters were similar for both groups. a significant decrease in hemoglobin level and saturation of transferrin was observed at 10 months in ...	2007	2293270
[nephrotic syndrome].		2008	2293261
regulation of metallothionein gene expression.	the metallothioneins are small, cysteine-rich proteins that have the capacity for high affinity binding of heavy metal ions, and whose synthesis is regulated by metal ion concentrations. these properties suggest that they play pivotal roles in the metabolism of the relatively nontoxic essential metals (zinc and copper), as well as toxic heavy metals (cadmium), a concept supported by a variety of studies of cells in culture, as well as in intact animals. expression of the metallothionein genes ma ...	2008	2293243
preliminary observations on the phenotypes of lymphoid cells in mycosis fungoides using anti-human-thymocyte monoclonal antibodies.	the phenotypes of lymphoid cells from twelve patients with mycosis fungoides (including 5 patients in the tumor stage and 7 in the pretumor stage) were determined immunohistochemically using anti-thymocyte monoclonal antibodies (hit1, hit2, and leu 6). the results showed significant differences between the numbers of hit1 and hit2 positive cells in tumor and pretumor stage lesions, indicating that these two monoclonal antibodies may be of help in the staging of mycosis fungoides and in making a ...	1990	2293225
release of human serum albumin from poly(lactide-co-glycolide) microspheres.	human serum albumin (hsa) was encapsulated in a 50:50 copolymer of dl-lactide/glycolide in the form of microspheres. these microspheres were used as a model formulation to study the feasibility of controlling the release of large proteins over a 20- to 30-day period. we show that hsa can be successfully incorporated into microspheres and released intact from these microspheres into various buffer systems at 37 degrees c. a continuous release of the protein could be achieved in physiological buff ...	1990	2293220
a sensitive and specific liquid-chromatographic assay for determination of ganciclovir in plasma and urine and its application to pharmacokinetic studies in the rabbit.	a liquid-chromatographic assay for the analysis of ganciclovir in plasma and urine is described. this assay involves the use of acyclovir, an antiviral drug structurally related to ganciclovir, as the internal standard. a two-step sample preparation method is used. after protein is precipitated with acetonitrile and the addition of diethyl ether, ganciclovir and the internal standard are back extracted into a small volume of aqueous ammonium phosphate, taking advantage of their relatively high w ...	1990	2293208
patch-clamp recordings on rat cardiac muscle slices.	thin slices (100-200 microns) from cardiac ventricles of newborn (1-14 days old) rats were used for patch-clamp recordings. high resistance seals (greater than 10 gohms) could be obtained on this preparation without enzymatic treatment of tissue. resting potential for cardiac fibres, measured in whole-cell configuration, ranged between -30 and -65 mv. averaged sodium currents and inward rectifying potassium single-channel currents, recorded in cell-attached mode, displayed similar features to th ...	1990	2293201
raw scores vs percentage conversions in factorial solutions for projective test variables.	factor analytic solutions for raw scores and corresponding percentage conversion scores derived from rorschach and hand test variables were directly compared. number of factors, total and individual communalities, and test overlap were sufficiently different to question either approach as preferable for summarizing interrelationships among projective tests and test variables. most of the substantial loadings tended to be either artifactual, relatively uninformative, or appeared on one factor ana ...	2008	2293185
a sex difference in field dependence/independence in the absence of vestibular activation and eye movements.	frame influence on perception of target orientation was investigated using small stimuli presented briefly to central vision. college men and women were equally accurate in their perceptions of the orientations of alphanumeric characters presented without a frame. when the same targets were enclosed in a frame, the performance of women, but not men, was significantly impaired.	2008	2293178
[method of modelling an ulcerative process in the stomach].		1990	2293167
[features of oxygen supply in exercise load in patients with atherosclerosis obliterans of the peripheral arteries].	the organism's oxygen balance was studied in 27 patients with atherosclerosis of the peripheral arteries during the test on a bicycle ergometer. the decrease in the patient's physical capacity was found to be in high correlation with the severity of oxygen disbalance.	2008	2293156
lumbar intrathecal administration of naloxone antagonizes analgesia produced by electrical stimulation of the hypothalamic arcuate nucleus in pentobarbital-anesthetized rats.	in lightly pentobarbital-anesthetized and acutely-prepared rats, electrical stimulation within the arcuate nucleus of the hypothalamus consistently inhibited the tail-flick responses to noxious heating of the tail. the opioid receptor antagonist, naloxone hydrochloride applied intrathecally at the lumbar level, at dose of 20 micrograms, reversed this inhibition without affecting the baseline pain threshold. the same dose of naloxone, applied to the cervical subarachnoid space, had no effect on t ...	1990	2293056
assay of all-trans----11-cis-retinoid isomerase activity in bovine retinal pigment epithelium.		1990	2292965
[human immunodeficiency virus infection associated with tuberculosis].	in order to detect an association between hiv infection and tuberculosis (tb), 130 tb inpatients were studied one of whom presented a pulmonary disease due to mycobacterium avium intracellulare. all had advanced tb, 95.4%, with pulmonary localization. serum anti-hiv antibodies were detected by elisa and their presence confirmed by immunoblotting in 4 (3.1%) individuals, three males and one female, with different degrees of pulmonary tb. of the males, 1 was bisexual, 2 were promiscuous, and the f ...	1990	2292908
a sensitive in vitro functional assay to detect k(+)-channel-dependent vasodilators.	this study describes a sensitive in vitro relaxation assay using isolated rabbit mesenteric artery to detect the activity of a vasodilator as a k(+)-channel activator. thus, comparison of several known k(+)-channel activators was made with other vasodilators known to work via various cellular mechanisms. the vasodilators used were minoxidil sulfate (mnxs; 5 microm), brl-34915 (cromakalim, 0.1 microm), nicorandil (10 microm), pinacidil (1 microm), diazoxide (100 microm), sodium nitroprusside (10 ...	1990	2292878
[technical problems of various sanitary-hygienic aspects of a medical unit on space aircraft].		2008	2292877
[a case of asymptomatic course of kidney tumor].		2008	2292876
[principles of creating esophageal anastomoses].	the techniques of formation of esophageal anastomoses are discussed on the basis of experience with 317 operations. the authors believe the following moments to be most important in the creation of communications with the esophagus: the use of a laser scalpel and monophilic atraumatic suture material, accurate apposition of the identical coats of the anastomosed organs by means of sutures placed at some distance from one another and tied not too tightly, creation of an antireflux mechanism in fo ...	2008	2292863
[endoscopic laser interventions in lung cancer].	endoscopic laser operations were conducted in 156 patients with various tumors of the trachea and bronchi. the indications for laser operations were neoplastic stenosis of the respiratory tract, bleeding from the tumor, and recurrent carcinoma in a bronchial stump after previous operations. neodymium laser with a monofiber quartz light guide was the source of laser radiation. laser photodestruction was performed through a fiberoptic bronchoscope in 34 patients and by a fridel respiratory broncho ...	2008	2292862
[performing lympho-venous anastomoses at several levels of the lower extremity in primary lymphedema].	operations were conducted on 41 patients, 31 of them had a primary form of lymphedema. lymphovenous anastomoses (lva) were formed in the region of the medial collector on the thigh in scarpa's triangle, in the middle third of the leg, and on the dorsum of the foot. the results were good in the late-term postoperative period in 74.0% of patients. according to earlier studies, the formation of lva in patients with primary lymphedema of the lower limbs only on one of the standard levels produces go ...	2008	2292856
[surgical treatment of occlusive diseases of the brachiocephalic arteries].	experience of the department of vascular surgery of the novgorod regional hospital from 1985 is analysed. the department has the standard sets of instruments, equipment, and roentgenodiagnostic apparatus which allow catheter, selective, and puncture angiography to be carried out. thirty-six operations were performed on 28 patients. all were conducted under endotracheal anesthesia with medicamentous protection of the brain. one patients died from reflex cardiac arrest. no complications occurred i ...	2008	2292854
[regional blood lactate level in arterial occlusive diseases].	fifty-seven patients with obliterating diseases of the lower limb arteries were examined. the concentration of lactate in the venous blood and the venous-arterial difference in the blood lactate level in the affected limb were found to be increased. a relationship between the marked character of these shifts and the severity of the ischemic syndrome was noted. the authors discuss the possibility of using the values of the lactate content in the regional blood as criteria for evaluating the effic ...	2008	2292851
[a clinico-pathological study of small renal cell carcinoma].	between 1960 and 1989, 50 patients with small renal cell carcinoma measuring below 3 cm in diameter were examined. among them, 31 patients were nephrectomized, but in 19 patients, the renal cell carcinoma was found first at autopsy. the average tumor diameter of the unrecognized ones was smaller than that of nephrectomized patients with the minimum of 0.4 cm. forty-one patients had clear cell carcinoma, 9 granular cell carcinoma, 40 grade 1 tumor, and 10 grade 2 tumor. the grade 3 tumor was not ...	2008	2292822
grading white matter lesions on ct and mri: a simple scale.	we developed and tested a simple three-point scale for grading white matter lesions in anterior and posterior regions of the brain. twenty four ct scans and 24 mri scans were separately judged by 11 and five observers, respectively, on the presence and severity of white matter lesions. the observers were radiologists and neurologists. for ct scans, these periventricular changes were graded according to their extent as absent, or partly involving the white matter, or extending up to the subcortic ...	2008	2292703
chemical modification of pig liver initiation factor eif-2 with n-ethylmaleimide. amino acid sequences around the n-ethylmaleimide-reactive sulfhydryl groups and the effect of gdp on the modification.	the activity of eukaryotic initiation factor eif-2 as to the formation of the ternary complex, eif-2 gtp met-trna(f), is inhibited by n-ethylmaleimide. our preparation of pig liver eif-2 contained alpha and gamma subunits and was inhibited by more than 90% by n-ethylmaleimide. using our eif-2, we determined the sequences around the n-ethylmaleimide-reactive sulfhydryl groups, studied the effect of gdp on the sulfhydryl modification and that of nem on the [3h]gdp binding, and examined the protect ...	1990	2292592
generation of affinity for antithrombin iii by supplemental sulfation of heparin species with low affinity for the protein.	the tributylammonium salt of a porcine heparin subfraction with low affinity for antithrombin iii (mr 7,500-18,000; anti-clotting activity, 7 usp units/mg), having degrees of sulfate substitution at d-glucosamine and l-iduronic acid residues of glcns 0.786, glcn6s 0.628, and idoa2s 0.682 mol, was reacted with 10 or 20 mol of pyridine-sulfur trioxide per mol equiv. of available hydroxyl groups in n,n-dimethylformamide at -10 degrees c for 1 h. both chemical and nmr spectroscopic analyses revealed ...	1990	2292586
a case of chediak higashi syndrome.		2008	2292561
development and regulation of growth and differentiated function in human and subhuman primate fetal gonads.	we have attempted to summarize the research on primate fetal gonadal development that has occurred over the past three decades. many similarities exist between fetal gonadal development in human and subhuman primates; therefore, comparisons and analogies between these species can be made. fetal gonadal development is a complex process dependent on timely maturation and differentiation of several cell types with different functions. adequate development is important for normal sexual development ...	1990	2292242
neural transplantation in parkinson's disease: the swedish experience.		1990	2290977
neural transplantation in parkinson's disease: the swedish experience.		1990	2290977
[improvement of chemotherapy of primary forms of pulmonary tuberculosis in adolescents].	observation findings concerning 112 adolescents with tuberculosis are presented. the patients were divided into 2 groups. group 1 included 61 cases with primary forms of tuberculosis and without lung tissue destruction or bacillary excretion. the best part of this group was affected by tuberculosis of intrathoracic lymph nodes and focal tuberculosis. group 2 consisted of 51 patients with primary tuberculosis in addition to lung tissue destruction or bacillary excretion. most of these patients ha ...	2008	2290807
theophylline and isoniazid.		1991	2288839
growth hormone receptors in hypothalamic and extra-hypothalamic tissues.	central gh receptors (ghr) have been identified in hypothalamic and extra-hypothalamic tissues of rabbit and chicken brains. plasma membranes of the rabbit brain demonstrated specific saturable high-affinity, low-capacity binding sites for 125i-labelled gh. rna extracted from hypothalamic and extra-hypothalamic tissues of rabbit and chicken brains contained mrna that hybridized with a cdna probe for the rabbit liver ghr. this transcript was of a similar size to the major ghr mrna moiety in rabbi ...	1990	2288635
american medical association white paper on elderly health. report of the council on scientific affairs.		2008	2288622
[environmental pathology and dentistry. iii. carcinogenic process of the soft tissues of the oral cavity].	the context in which a relationship between environmental pathologies and dentistry is most easily distinguished is with reference to the carcinogenic processes of soft tissues in the oral cavity. in this study, a distinction is made between potentially cancerous lesions and true carcinomas, and the relationship between tumour pathology of the oral cavity and the surrounding environment is analysed. clinical aspects are also illustrated.	2008	2287330
antigenic and catalytic disparity in the distribution of cytochrome p-450-dependent 25-hydroxyvitamin d3-1 alpha- and 24-hydroxylases.	chick 25-hydroxyvitamin d3-1 alpha-hydroxylase, a cytochrome p-450 monooxygenase with a molecular weight of 57 kda, can be isolated as described by mandel et al. (1990 b). under normal physiological circumstances, it occurs exclusively in kidney mitochondria. an isozyme of the 1 alpha-hydroxylase, known as the 24-hydroxylase, which uses the same substrate to yield an isomeric product, is also a cytochrome p-450 monooxygenase, has a molecular weight of 55 kda, and like-wise occurs in kidney mitoc ...	1990	2286531
non-o group 1 vibrio cholerae: a look at the epidemiology of an occasional pathogen.	non-o1 v. cholerae is a ubiquitous environmental isolate. it is a common contaminant of shellfish, and in the developing world, it is frequently isolated from food and water. asymptomatic carriage rates approaching 4 percent have been described among persons involved in high-risk activities, such as eating oysters in new orleans or going on pilgrimage to mecca. the actual occurrence of disease appears to be much less common. this is an "occupational" pathogen which may be responsible for outbrea ...	1990	2286218
clonal diversity of vibrio cholerae o1 evidenced by rrna gene restriction patterns.	the rrna gene restriction patterns of 89 vibrio cholerae o1 isolates from different geographic origins were studied. the probe was escherichia coli 16 + 23s rrna labelled with "ecl gene detection system". a total of 17 rrna gene restriction patterns were observed after bgli cleavage. four patterns (b1 to b4) were only given by biotype cholerae (14 strains studied). thirteen patterns (b5 to b17) were only given by biotype el tor (75 strains studied). there was no correlation between serotypes and ...	1990	2284500
electron microscopic study of the polymyxin treated vibrio cholerae cells.	polymyxin b produces dose dependent changes in the surface topography of pathogenic vibrio cholerae cells. the susceptibilities of various vibrio strains to pb are also studied through analytical techniques. statistical analysis shows significant differences among the four vibrios with regard to their sensitivities to pb, the classical strains being the most sensitive. treating the classical strain with subinhibitory concentration of pb, we observed with both sem and tem that the normal smooth s ...	1990	2282116
genetic transformation of vibrio parahaemolyticus, vibrio alginolyticus, and vibrio cholerae non o-1 with plasmid dna by electroporation.	an electroporation procedure for the plasmid-mediated transformation of the genus vibrio was performed, as part of an effort to develop recombinant dna techniques for genetic manipulation of the genus vibrio. vibrio parahaemolyticus, v. alginolyticus, and v. cholerae non o-1 (9 different strains) were transformed with 3 vector plasmids (pacyc184, phsg398, and pbr325). the efficiency of transformation was highly dependent on three parameters: the concentration of plasmid dna; the strength of the ...	1990	2280728
toxoplasma antigens recognized by human immunoglobulin a antibodies.	the immunoglobulin a (iga) antibody response to antigens of toxoplasma gondii during the acute and chronic stages of t. gondii infection was studied by immunoblotting with a nonreduced antigen (nra) preparation. serum samples were from individuals with acute or chronic t. gondii infection, and sequential samples were from women who seroconverted during gestation or congenitally infected infants. iga antibodies to a variety of antigens were present in sera from each of the groups, irrespective of ...	1990	2279993
partial characterization of a highly conserved aspartyl kinase (ak) in normal human liver.	subcellular fractions from human liver were assayed for aspartyl kinase (ak) activity measured by standard spectrophotometric methods. along the purification procedure three different fractions displayed the expected enzyme activity. interestingly, two of these fractions were specifically recognized by antibodies raised against e. coli aspartate kinases, suggesting a high degree of evolutionary conservation for these ubiquitous enzymes for prokaryotes. since their known function in bacteria is n ...	1990	2279261
[prognosis-oriented diagnosis, treatment protocol and after care in uretero-vaginal fistula following gynecological surgery from the urological viewpoint].	it is reported on 20 cases of ureterovaginal fistula after hysterectomy. in all cases renal protection (nephrostomy) was compulsory. spontaneous healing without stenosis occurred in 6 cases, in 10 cases urethral reimplantation was successful, in 4 cases nephrectomy had to be carried out. tactics of the therapy are described from the urologic viewpoint. pre- and postoperative marks with relevance for prognosis and the special following-up are especially emphasized in this connection.	2008	2278219
[pregnancy outcome following cerclage. 2. multiple pregnancy].	in a retrospective study pregnancy and labour after cerclage had been studied in comparison to 160 patients with singleton pregnancies and a matched pair control group of multiple pregnancies without operative cervix closure. cervical incompetence in multiple pregnancy observed more frequently than in singleton ones is not correlated to obstetric history, but has a bad prognosis because of necessity of tocolysis during pregnancy, marked shortening of duration of pregnancy, lowering of length and ...	2008	2278218