Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| frequency analysis of simian virus 40-specific cytotoxic t lymphocyte precursors in the high responder c57bl/6 mouse strain. | studies in this laboratory have shown that long term simian virus 40 (sv40)-specific cytolytic t lymphocyte (ctl) cultures established from the spleens of high responder c57bl/6 (b6; h-2b) mice exhibit a preference for the selection of h-2db-restricted ctl clones. in this study, we have investigated the basis for this selection. limiting dilution cultures were established using responder cells from the popliteal lymph nodes and the spleens of b6 mice immunized subcutaneously in the hind footpads ... | 1988 | 2459302 |
| distinct domains of recombinant human ifn-gamma responsible for anti-viral effector function. | a panel of 21 independently isolated neutralizing mab directed against the human rifn-gamma (rhuifn-gamma) was used to characterize those epitopes that are involved in the antiviral function of the rhuifn-gamma. a sandwich competition assay was developed to determine the cross-reactivities between the mab. the 125i-labeled mab were allowed to compete with varying amounts of unlabeled mab for binding to rhuifn-gamma under ag-limiting conditions, and the 50% inhibition endpoints were determined fo ... | 1988 | 2459212 |
| adjuvant effect of deae-dextran on cholera vaccines. | investigations into the effectiveness of deae-dextran as an adjuvant of whole cell cholera vaccines (vibrio cholerae inaba and ogawa serotypes) were conducted using two methods: (a) the active mouse protection test, (b) the determination of antibody production in the sera of immunized mice by measuring the level of vibriocidal antibodies, and by an enzyme-linked immunosorbent assay (elisa). in all tests, the deae-dextran-adjuvanted vaccine showed an antigenicity substantially superior to that ac ... | 1988 | 2458658 |
| transfer of antibiotic resistances from bacillus spp. to staphylococcus aureus and vibrio cholerae. | 1988 | 2456988 | |
| virus enhances histamine release from human basophils. | histamine release from human basophil leukocytes was triggered by staph. aureus or by complement activation caused by endotoxins isolated from e. coli or salmonella bacteria. influenza a virus was found to enhance the mediator release and the effect was caused by synergism, since the virus itself did not release histamine. the potentiating effect of the virus was abolished by a potent neuraminidase inhibitor. furthermore, a purified neuraminidase preparation obtained from vibrio cholerae caused ... | 1988 | 2455978 |
| vibrio fluvialis: a new serogroup (19) possessing the inaba factor antigen of vibrio cholerae o1. | a serogroup of vibrio fluvialis possessing the c (inaba) antigen but not the b (ogawa) nor a antigen of v. cholerae o1 is described. the o-antigen of this serogroup was identical with that of bioserogroup 1875-variant of a marine vibrio species. as the o-antigen of this serogroup was not agglutinated by any of o-antisera for the 18 serogroups of v. fluvialis already recognized, it was designated o-serogroup 19 of this species. | 1987 | 2455085 |
| [characteristics of the formation of hydrogen sulfide by cholera vibrios]. | 1988 | 2453707 | |
| doxepin-induced cardiac dysrhythmia. | 2008 | 2453531 | |
| nucleotide sequences and comparison of the hemolysin determinants of vibrio cholerae el tor rv79(hly+) and rv79(hly-) and classical 569b(hly-). | we determined the nucleotide sequences of the hemolysin structural gene, hlya, of vibrio cholerae el tor biotype strains rv79(hly+) and rv79(hly-) and the hly determinant of the nonhemolytic classical biotype strain 569b(hly-). the sequences of the hlya gene from el tor strains rv79(hly+) and rv79(hly-) have an identical 2,223-base open reading frame which is predicted to encode an 81,977-dalton precursor form of hemolysin. this value is in excellent agreement with the 84,000-mr hemolysin descri ... | 1988 | 2453464 |
| analysis of amplitude and area of concentric needle emg motor unit action potentials. | computer simulations indicate that measurements of the area of motor unit action potentials (muaps) recorded with a concentric needle electrode could be useful in differentiating between neuropathy and myopathy. however, muap area varies markedly when the position of the recording electrode is changed only slightly within the motor unit territory, mainly because of the changes in the muap amplitude produced by only slight electrode movements. the ratio of muap area to amplitude is much less affe ... | 2008 | 2453333 |
| recombinant gibbon interleukin-3 acts synergistically with recombinant human g-csf and gm-csf in vitro. | recombinant gibbon interleukin-3 (il-3) is a multilineage hematopoietic colony-stimulating factor (csf) that recently was cloned and found to be highly homologous with human il-3. gibbon il-3, as well as human granulocyte-csf (g-csf) and human granulocyte-macrophage csf (gm-csf), stimulated normal human bone marrow cells to form myeloid colonies in soft agar in a sigmoidal dose-response manner. when il-3 was added to increasing concentrations of g-csf or gm-csf, synergistic colony formation occu ... | 1988 | 2453230 |
| influenza a virus potentiates basophil histamine release caused by endotoxin-induced complement activation. examination of normal individuals and patients with intrinsic asthma. | histamine release from human basophil leukocytes was triggered by complement activation by means of endotoxins isolated from e. coli and salmonella bacteria. influenza a virus was found to enhance the mediator release, and the effect was caused by synergism, since virus itself did not release histamine. the potentiating effect was similar in cells from normal individuals and from patients with intrinsic asthma. the involvement of viral neuraminidase was examined by a potent neuraminidase inhibit ... | 1988 | 2452582 |
| demonstration of lipopolysaccharide on sheathed flagella of vibrio cholerae o:1 by protein a-gold immunoelectron microscopy. | monoclonal antibodies with group and type specificity for lipopolysaccharide antigens were used in combination with protein a-colloidal gold labeling and transmission electron microscopy to demonstrate the presence of lipopolysaccharide antigens on both the sheathed flagellum and the cell surface of inaba and ogawa strains of vibrio cholerae o:1. labeling was associated with the sheath of the flagellum rather than the core, and flagellar cores were not labeled. flagellum and cell shared a common ... | 1988 | 2450866 |
| purification and partial characterization of fimbriae of vibrio cholerae o1. | fimbriae of vibrio cholerae o1 were purified from a strain of the classical biotype, inaba serotype (bgd 17), and a strain of the el tor biotype, ogawa serotype (k23), grown on tcg agar medium by the following procedure; homogenization of the cell suspension to detach fimbriae, ultracentrifugation to remove remaining cells and their debris, concentration of the supernatant containing fimbriae with ultrafiltration, and 20 to 40% sucrose linear gradient centrifugation of the concentrated material. ... | 1987 | 2448968 |
| branch retinal vein occlusion. a clinicopathologic case report. | the eyes of a 72-year-old woman with a history of two branch retinal vein occlusions involving the left eye, were obtained postmortem and studied histopathologically. prior to her death, she had been enrolled in the multicentered, prospective, randomized clinical trial on branch vein occlusion and treated with peripheral argon laser photocoagulation for disc neovascularization. routine fluorescein angiograms and fundus photographs were available for clinical correlation. despite photocoagulation ... | 2008 | 2448855 |
| studies on the vibrio cholerae mucinase complex. iii. neutralisation of the neuraminidase activity by specific anti-neuraminidase igg. | a partially-purified neuraminidase from the mucinase complex of vibrio cholerae was used to prepare a specific anti-neuraminidase antiserum in rabbits. when the neutralising potency of this serum against v. cholerae neuraminidase was assessed in conventional tests, the enzymic activity, as measured by thiobarbituric acid, methoxyphenol-neuraminate and goblet-cell assays, apparently increased. these results are attributable to the presence of a sialylated glycoprotein substrate and small amounts ... | 1988 | 2448460 |
| determination of the functional affinity of igg1 and igg4 antibodies to tetanus toxoid by isotype-specific solid-phase assays. | two solid-phase assays have been used to measure the affinity of igg1 and igg4 antibodies to tetanus toxoid (tt) in whole sera. in the first assay, low affinity antibodies were selectively inhibited from binding to solid-phase antigen by a mild chaotropic agent and in the second assay, high affinity antibodies were preferentially inhibited from binding by low concentrations of free antigen. results from both assays suggest that igg4 antibodies to tt are of lower functional affinity than igg1 ant ... | 2000 | 2448383 |
| different types of monoclonal antibodies to ogawa-specific and group-specific antigens of vibrio cholerae o1. | nine monoclonal antibodies to ogawa-specific antigenic determinants of vibrio cholerae o1 and seven monoclonal antibodies to the ogawa-inaba common antigenic determinants were obtained. specificities and reactivities were examined by slide or microdilution agglutination methods, along with enzyme-linked immunosorbent assays and immunoblotting analysis. in both the ogawa-specific and ogawa-inaba common groups, it was revealed that there were two types of antibodies. one type showed strong aggluti ... | 1987 | 2448333 |
| ca dependence of na influx during treatment of rabbit aorta with ne and high k solutions. | cellular influx of 24na was measured in isolated rabbit aorta during stimulation with 10 microm norepinephrine (ne) or depolarization with 80 mm k solution, using a pulse-labeling, cold-wash technique. ne caused a two- to threefold increase in na influx; a smaller but significant increase was also observed in depolarized tissues. basal and ne-induced fluxes at 1 min were significantly increased by a 20-min preincubation in a ca-free solution containing 2 mm egta; elevation of [mg] in this soluti ... | 1988 | 2447797 |
| the distribution of hla-dr determinants, secretory component (sc), amylase, lysozyme, iga, igm and igd in fetal and postnatal salivary glands. | 2008 | 2447762 | |
| the potential value of the demonstration of thyroglobulin by immunoperoxidase techniques in fine needle aspiration cytology. | the peroxidase-antiperoxidase method for the demonstration of thyroglobulin was performed on 30 fine needle aspiration smears. all of the benign thyroid lesions plus the papillary and follicular carcinomas of the thyroid were positive for thyroglobulin. two cases of metastatic thyroid carcinoma in lymph nodes were also positive. medullary carcinoma of the thyroid and ten control smears from various other tissues were negative. this proves the sensitivity and the specificity of the method, which ... | 2008 | 2447717 |
| a protein preparation from mycobacterium kansasii culture filtrate has biological activity similar to that of hcg and cholera toxin in human cell lines. | a protein preparation, from culture filtrates from a strain of mycobacterium kansasii (mk precipitate), which cross reacts with antibodies to human chorionic gonadotropin (hcg) beta subunit and antibodies to cholera toxin beta subunit, has been isolated. a tissue culture assay was used to detect the ability of this preparation to affect the antiviral activity of interferons and to visualize changes in cell shape and cell-cell contact caused by this preparation. a cholera toxin containing precipi ... | 1987 | 2447470 |
| chinese hamster ovary cell lysosomes rapidly exchange contents. | we have used cell fusion to address the question of whether macromolecules are rapidly exchanged between lysosomes. donor cell lysosomes were labeled by the long-term internalization of the fluid-phase pinocytic markers, invertase (sucrase), lucifer yellow, fitc-conjugated dextran, or texas red-conjugated dextran. recipient cells contained lysosomes swollen by long-term internalization of dilute sucrose or marked by an overnight fitc-dextran uptake. cells were incubated for 1 or 2 h in marker-fr ... | 1987 | 2447096 |
| lectin binding to human endometrial hyperplasias and adenocarcinoma. | arias-stella change, cystic glandular hyperplasia, adenomatous hyperplasia, atypical hyperplasia, and adenocarcinoma of the human endometrium were stained with different fluorescein-conjugated lectins. the lectins used were concanavalin a, triticum vulgaris, arachis hypogaea, glycine maximum, dolichos biflorus, ulex europaeus, and ricinus communis. the results showed that the lectin staining of arias-stella change differed from the other conditions and resembled more closely the staining propert ... | 1987 | 2447025 |
| determinants of immunogenicity and mechanisms of protection by virulent and mutant vibrio cholerae o1 in rabbits. | the colonizing capacities of 16 vibrio cholerae strains, including nine genetically and/or phenotypically defined parent-mutant pairs, were determined in unobstructed adult rabbit small bowel. there were marked interstrain differences in colonizing capacity, which was enhanced by bacterial motility and the production of cholera holotoxin but was unrelated to production of cholera toxin b-subunit or hemolysin or to bacterial serotype or biotype. the role of colonizing capacity and other bacterial ... | 1988 | 2447017 |
| repetitive natriuresis and blood pressure. long-term calcium entry blockade with isradipine. | the long-term effects (3.5 months) of a new calcium entry blocker of the 1-4-dihydropyridine class, isradipine (pn 200-110), on renal hemodynamics and excretional parameters were investigated in 10 essential hypertensive subjects (world health organization classes i and ii). blood pressure and renal vascular resistance fell significantly (p less than 0.001), and a slight increase in glomerular filtration rate and renal plasma flow was seen (p less than 0.05). output of fluid from the proximal tu ... | 2008 | 2447016 |
| effects of substance p, neurokinin a and calcitonin gene-related peptide in human skin and their involvement in sensory nerve-mediated responses. | the effects evoked by intradermal injections of substance p (sp), neurokinin a (nka) or calcitonin gene-related peptide (cgrp) were studied in 51 non-atopic subjects. sp and nka produced flare and weal, and cgrp produced an indurated erythema. the reactions to sp were strong, the flare being maximal 3-5 min after injection and the weal after 10-15 min. nka evoked a much weaker flare and a slightly weaker weal than did sp. cgrp produced a prominent long-lasting, indurated erythema with pseudopod ... | 1987 | 2446892 |
| ca-125 and endometriosis. | 2008 | 2446822 | |
| antiviral chemotherapy and prophylaxis of viral respiratory disease. | respiratory viruses continue to be major causes of morbidity and mortality. currently available chemotherapy is limited to oral amantadine for uncomplicated influenza a and aerosolized ribavirin for respiratory syncytial virus (rsv) infections. amantadine is also efficacious for chemoprophylaxis of influenza a virus infections. rimantadine has similar clinical efficacy and is better tolerated than amantadine. aerosolized ribavirin may be useful in the treatment of serious respiratory illness cau ... | 1987 | 2446820 |
| autism and plumbism. a possible association. | six cases of inner city black children with both infantile autism and lead poisoning are reviewed. in three cases, developmental deviance seems to have been present before the possible impact of lead toxicity. in two cases the lead poisoning may have contributed to the onset or acceleration of developmental symptomatology. in one case the temporal sequence remains unclear. possible patterns of interaction and the implications for clinical practice are discussed. | 2008 | 2446813 |
| immunosuppressive effect of fk506 on collagen-induced arthritis in rats. | fk506, a new immunosuppressive agent, was given intramuscularly to rats for 12 days, starting on the day of type ii collagen immunization. fk506 in doses of 0.32 mg/kg or more suppressed arthritis and also suppressed humoral and skin test response to type ii collagen. fk506 suppressed arthritis only when given during the afferent limbs of immune response (0-4 days), whereas the drug was only marginally effective when treatment was started during the efferent limbs of immune response (7-11 days). ... | 1988 | 2446810 |
| characteristics of antibodies adsorbed on the dna immunoadsorbent, agarose poly-l-lysine-dna. | agarose-poly-l-lysine (ag-(lys)n-dna) has been used to bind dna for assay of anti-dna antibodies (ab). in this work, an algorithmic approach has been used to classify antinuclear ab (ana) as being directed against native dna (dsdna), denatured dna (ssdna), dna-protein complexes (deoxyribonucleoprotein; dnp), and against antigens which are independent of dna (idna). these ab were subjected to ag-(lys)n-dna, and the selectivity of this adsorbent for the various specificities of ab was determined. ... | 2008 | 2446798 |
| regulation of cell growth by interferon. | interferons can regulate growth and differentiation in a wide range of cell types. these mechanisms are currently being examined. interferons inhibit the growth of tumour cells and are thus potential anti-cancer agents. they can also inhibit normal cell growth in vitro, and stimulate tumour cell growth in vitro. they may also be involved in some autoimmune diseases. this review examines the effect of interferons on cell proliferation, function, and growth, focusing primarily on in vitro cell sys ... | 2008 | 2446790 |
| effect of endogenous and exogenous interferons on the differentiation of human monocyte cell line u937. | the effect of interferons (ifns) on the differentiation of hematopoietic cells was examined with the human monocyte cell line u937. the differentiation of u937 was induced by hydroxyvitamin d3 and was evaluated through the study of specific markers. the induction of the u937 differentiation was associated with a production of ifn and with a marked increase in (2'5') oligoadenylate synthetase. addition of anti-ifn-alpha/beta antibodies inhibited the enhancement of (2'5') oligoadenylate synthetase ... | 1988 | 2446743 |
| calcium channels and excitation-contraction coupling in cardiac cells. ii. a pharmacological study of the biphasic contraction in guinea-pig papillary muscle. | biphasic contractions were obtained in guinea-pig papillary muscle by inducing partial depolarization in k+-rich solution (17 mm) in the presence of 0.3 microm isoproterenol. mn2+ ions inhibited the two components of contraction in a similar way. nifedipine and particularly cd2+ ions specifically inhibited the second component of contraction. isoproterenol and bay k 8644 markedly increased the amplitude of the second component (p2) of contraction. nevertheless, a moderate positive inotropic effe ... | 1987 | 2446724 |
| distributions of certain neuropeptides in the primate thalamus. | the distributions of fibers and terminals immunoreactive for somatostatin (srif), neuropeptide y (npy), substance p (sp) and cholecystokinin octapeptide (cck), were studied in the diencephalon of cynomolgus monkeys. immunoreactivity for all 4 peptides is found in extrinsic afferent fibers innervating the dorsal thalamus, ventral thalamus and epithalamus. the distributions of such fibers are more extensive than previously described and include many relay nuclei in their zones of terminations. sp ... | 1987 | 2446708 |
| maitotoxin-induced membrane current in neuroblastoma cells. | maitotoxin (mtx) is a potent marine toxin isolated from the toxic dinoflagellate, gambierdiscus toxicus. we have examined the possibility of mtx activating calcium channels using cultured neuroblastoma cells (n1e-115). mtx (10 ng/ml) produced a depolarization of the membrane, which was prevented by the removal of ca2+ from the external medium. under voltage clamp conditions, membrane currents were recorded with 50 mm ba2+ as a charge carrier through calcium channels. after application of mtx (1 ... | 2008 | 2446704 |
| definition of myasthenogenic sites of the human acetylcholine receptor using synthetic peptides. | experimental autoimmune myasthenia gravis (eamg) and antibodies that modulate achrs from cultured human muscle are induced by a disulfide-looped peptide comprising the human acetylcholine receptor (achr) alpha-subunit residues 125-147 (h alpha 125-147). to delineate the essential antigenic requirements for induction of eamg by this peptide, a series of peptides was synthesized: (a) a nonlooped analog (cys 128 replaced by ser) stimulated modulating autoantibodies, induced eamg, and bound antibodi ... | 1987 | 2446556 |
| antibodies to the insulin receptor as tools in the study of receptor structure and function. | 1987 | 2446553 | |
| evolution and development of the outer acrosomal membrane (oam) and evidence that acrosin-inhibitors are proteins of the oam. | an antiserum to the purified porcine outer acrosomal membrane (oam) was raised in rabbits and the igg fraction isolated by ammonium sulphate precipitation and ion exchange chromatography. the antibodies reacted exclusively with the acrosomal cap of the sperm head as revealed by indirect immunofluorescence. in addition they cross-reacted not only with the acrosomal part of the spermatozoa of all mammalian species tested (bull, horse, rabbit, rat, mouse, hamster, mole, antelope, monkey, man) but a ... | 1987 | 2446533 |
| improved synthesis and iodination of a cleavable photoactivated probe. | j. b. denny and g. blobel (1984, proc, natl. acad. sci. usa 81, 5286-5290) have described the synthesis of a water-soluble novel heterobifunctional 125i-labeled photocrosslinking reagent, n-[4-(p-azido-m-[125i]iodophenylazo)benzoyl]-3-aminopropionyl-n'- oxysulfosuccinimide, which contains a cleavable internal azo bond. we report several modifications of their synthesis which greatly increase the yield of a synthetic intermediate, n-[4-(p-aminophenylazo)benzoyl]-3-aminopropionic acid (compound vi ... | 2008 | 2446528 |
| [effect of dna and rna in foods on serum uric acid concentration in the human]. | nutritional experiments with formula diets show, that dna taken orally raises the serum uric acid concentration only half as much as does the same quantity of rna. in evaluating the suitability of foods used in the diet of patients with gout, however, food is only judged by its total purine-n content. in commercially available raw food the total purine content was determined enzymatically as uric acid. in the same samples the content of rna, dna, nucleotides, nucleosides and bases was determined ... | 1987 | 2446437 |
| cystic neoplasms of the pancreas. | eight patients with cystic neoplasms of the pancreas were seen at four northern california hospitals between the years 1978 and 1986. three of the tumors were benign and five were malignant. three females, whose average age was 61 years, had cystadenomas. three females and two males, whose average age was 48 years, had mucinous cystadenocarcinomas. clinical presentations were similar among all patients. abdominal pain was a prominent feature. anorexia, weight loss, nausea and vomiting with a pal ... | 2008 | 2446398 |
| antigenic determinants of the cholera/coli family of enterotoxins. | hybridoma-derived monoclonal antibodies were raised to enterotoxins of the cholera family and to chimeric b-subunit proteins in which individual amino acid residues of a heat-labile, cholera-related enterotoxin from an escherichia coli strain of porcine origin (p-lt) were substituted with corresponding residues from such an enterotoxin from an e. coli strain of human origin (h-lt). single amino acid substitutions were found to have profound effects on the physicochemical behavior of the proteins ... | 1987 | 2446368 |
| [in vitro preparation of enterotoxigenic species of vibrio cholerae 01 from nontoxigenic cultures of cholera and nag vibrios]. | 1987 | 2444761 | |
| analysis of human antibody response to cholera vaccination by epstein barr virus transformation and human-human hybridoma production. | the response of epstein barr virus transformable b lymphocytes to v. cholerae serotypes inaba and ogawa was assessed at various periods of time after primary and secondary immunization. in both cases, 100% of cells laid down at 10(4)/well gave a very strong positive reaction with either serotype one week after immunization. further analysis indicated that general polyclonal activation by the bacterial lps was not a major feature since the reactivity with other bacterial antigens was weak. human ... | 1987 | 2444705 |
| a physical map of the chromosomal region determining o-antigen biosynthesis in vibrio cholerae o1. | we have previously described the cosmid cloning of the genes determining the biosynthesis of the inaba and ogawa o-antigens of the lipopolysaccharides of vibrio cholerae o1 (manning et al., 1986). by southern hybridization analysis of chromosomal and cosmid dna, and heteroduplex analysis between the clones we have been able to precisely define the region of contiguous chromosomal dna in the vicinity of the o-antigen-encoding region. these data and comparison of end points of clones and of deleti ... | 1987 | 2444496 |
| [control of early pregnancies by vaginal sonography]. | 151 patients with suspected early pregnancies between 5 to 10 weeks were examined by transvaginal section scanning. pregnancies could be verified as early as 16 days after conception while the first heart beat actions were seen after 21 to 22 days. in early pregnancies where heart actions could not be shown at first examination a second look was taken after the 6. week dated from conception. beta-hcg-units were found in a range of 59 to 55,000 mu/ml. 3 pregnancies in the 5th, 6th, respectively 7 ... | 1987 | 2444038 |
| [immunostimulation in the lymphadenopathy syndrome and in aids--inosiplex (inosin pranobex, methisoprinol]. | in patients with aids, las and arc a defect in producing lymphokines such as interleukin 2 and gamma-interferon is found. possibilities of therapy are reported. especially the immunostimulant properties of inosiplex are commented, and the first successful results of therapy are discussed. | 2008 | 2443884 |
| isolation and structural analysis of the escherichia coli trp leader paused transcription complex. | transcription pausing is a key step in many prokaryotic transcription attenuation mechanisms. pausing is thought to occur when an rna hairpin forms near the 3' end of a growing transcript. we report here the isolation of the trp leader paused transcription complex containing a defined 92-nucleotide nascent transcript. digestion of isolated paused complexes with rnase t1 suggests that the trp leader rna hairpin designated 1:2 forms in the paused transcription complex. the transcription factor nus ... | 1987 | 2443722 |
| translation framing code and frame-monitoring mechanism as suggested by the analysis of mrna and 16 s rrna nucleotide sequences. | protein coding sequences carry an additional message in the form of a universal three-base periodical pattern (g-non-g-n)n, which is expressed as a strong preference for guanines in the first positions of the codons in mrna and lack of guanines in the second positions. this periodicity appears immediately after the initiation codon and is maintained along the mrna as far as the termination triplet, where it disappears abruptly. known cases of ribosome slippage during translation (leaky frameshif ... | 1987 | 2443708 |
| topological mapping of complement component c9 by recombinant dna techniques suggests a novel mechanism for its insertion into target membranes. | cdna molecules coding for mouse and trout c9 have been isolated and the derived amino acid sequences compared with that of human c9. regions of high homology between the closely related species (mouse and human) correlate with putative domains in the protein structure supporting a model of c9 having five globular domains. comparison between the more distant species (trout and human) suggests regions of particular importance to c9 structure and function. in addition the three related sequences al ... | 1987 | 2443347 |
| what's new in "in situ hybridization". | in situ hybridization (ish) represents a technique which allows the visualization of cellular dna or rna in tissue sections, single cell or chromosome preparations. in addition to the originally used nick-translated dna probes, synthetic oligodeoxyribonucleotides and single stranded cdna probes are applied to ish. with the more recent introduction of single stranded antisense rna probes a highly specific and more sensitive technique became available. besides the labeling of probes by the incorpo ... | 2008 | 2442734 |
| contradictory responses in induction of delayed type hypersensitivity in orally immunized mice. | induction of delayed type hypersensitivity (dth) in mice fed with sheep red blood cells (srbc) and live vibrio cholerae (v. cholerae) both forcedly and ad lib was comparatively investigated. although suppression of dth to srbc or v. cholerae was induced in mice fed forcedly for 1 or 2 weeks, mice fed ad lib could produce the positive footpad reactions to antigens. furthermore, the suppression of dth in forcedly fed mice showed an antigenic specificity. these observations indicated that the induc ... | 1987 | 2442393 |
| prospectively randomized clinical trial of three intensive chemotherapy regimens for the treatment of advanced unfavorable histology non-hodgkin's lymphoma. | three hundred thirty-two eligible patients with advanced (ann arbor stage iii or iv) non-hodgkin's lymphoma of aggressive histologic subtype (rappaport classification diffuse histiocytic [dh], diffuse poorly differentiated lymphocytic [dpdl], diffuse mixed [dm], or diffuse undifferentiated [du]) were randomly assigned to receive induction chemotherapy with one of three intensive regimens in a clinical trial conducted by the eastern cooperative oncology group (ecog) between 1978 and 1983. chemoth ... | 2008 | 2442322 |
| neonatal jaundice and glucose-6-phosphate dehydrogenase deficiency in basrah. | in a study on a group of 186 newborn babies presenting with jaundice, erythrocyte glucose-6-phosphate dehydrogenase (g6pd) deficiency was detected in 95 (51%) of the patients. the incidence of severe hyperbilirubinaemia appeared to be much greater in g6pd-deficient infants (46%) than in infants who did not have the red cell defect (15%). no change was found in this association when abo incompatibility was excluded. phototherapy did not reduce the need for exchange transfusion, which was necessar ... | 2007 | 2441650 |
| the pattern of cardiovascular malformation in the charge association. | congenital heart disease occurred in 64% of patients with the charge (coloboma, heart disease, choanal atresia, retardation of postnatal growth and mental development, genitalia hypoplasia, and ear anomalies) association (55% of 67 previously described patients and 100% of 16 new patients). of those with congenital heart disease, 42% had conotruncal anomalies (tetralogy of fallot, double-outlet right ventricle, truncus arteriosus), and 36% had aortic arch anomalies (vascular ring, aberrant subcl ... | 2008 | 2441595 |
| [heterogeneity of sperm immobilizing antibodies in sera of sterile women]. | among 126 sterile women, 8 patients indicated sperm immobilizing (si) antibodies in their sera, while 13 and 57 patients showed positive results in sperm agglutination (sa) test and indirect immunofluorescence (if) tests, respectively. these results suggest that the antisperm antibodies detected by different methods are not identical and seem to be raised to different epitopes of sperm antigens. si antibodies were absorbed not only with human spermatozoa, but also with human seminal plasma (hsp) ... | 1987 | 2440964 |
| [changes in blood coagulation, thrombocyte function and vascular prostacyclin synthesis caused by magnesium sulfate]. | magnesium deficiency, prostacyclin deficiency and reduced prostacyclin sensibility of the thrombocytes may possibly play an important pathophysiological role in gestosis/pre-eclampsia. for this reason, we studied the influence of magnesium sulphate (in vitro 2-16 mval/l) on thromboelastogram, spontaneous and adp-induced thrombocyte aggregation, prostacyclin sensitivity of the thrombocytes and prostacyclin liberation from umbilical cord vessel preparations. the thromboelastogram showed an inhibit ... | 2008 | 2440758 |
| no evidence on significant roles of the prostaglandin-thromboxane and kallikrein-kinin system in the antihypertensive effect of mk 421 in rats made hypertensive by norepinephrine or vasopressin. | inhibition of angiotensin converting enzyme by mk 421 (6 mg/kg/day ip) induced a significant increase in urinary kinin excretion in norepinephrine-infused rats (1.8 mg/kg/day ip), whereas it had no effect on urinary prostaglandin e2 excretion. in contrast, mk 421 did not induced any significant changes in urinary kinin and prostaglandin e2 excretion in vasopressin-infused rats (7.2 u/kg/day ip). the simultaneous administration of indomethacin (10 mg/kg/day sc), oky 046 (12 mg/kg/day sc) or aprot ... | 1987 | 2440625 |
| methodology for high-resolution two-dimensional gel electrophoresis. | 2008 | 2440530 | |
| a mechanistic interpretation of the action of toxin ii from anemonia sulcata on the cardiac sodium channel. | cardiac sodium channels, modified by anemonia sulcata toxin ii, have been analyzed by the patch-clamp method. the open state of the modified sodium channels proved to be prolonged highly significantly and reopening from a closed state denoted c*-state frequently occurred, interrupted by silent periods, denoted i*-state. activation from the c*-state was apparently not affected by toxin action, whereas activation from the i*-state was markedly prolonged. upon higher depolarizations toxin-induced s ... | 1987 | 2440476 |
| epiglycanin-immunoreactive glycoproteins in mouse fetal tissues and fetal cells in culture. | fetal tissues from time-pregnant female a/j mice of 16- and 19-day pregnancies and from neonates 1 day after birth, as well as from fetal cells in culture, absorbed significant amounts of anti-epiglycanin antibody. detergent-solubilized glycoproteins, with epiglycanin activity, from fetal tissues and cells were separated by polyacrylamide gel electrophoresis, and the protein bands electroblotted onto a nitrocellulose gel. after the antigens were labeled with rabbit anti-epiglycanin antiserum and ... | 1987 | 2438443 |
| residual mass after chemotherapy for seminoma: changing concepts of management. | a residual mass after induction chemotherapy for advanced seminoma may present radiographically and clinically as either retroperitoneal fibrosis or a discrete mass. the former is nonresectable and often it is associated with negative pathological findings, whereas the latter is resectable but frequently it consists of viable seminoma, providing an indication for further therapy. | 2008 | 2438431 |
| isolation of novel human retrovirus-related sequences by hybridization to synthetic oligonucleotides complementary to the trna(pro) primer-binding site. | synthetic oligonucleotides complementary to putative retroviral primer-binding sites were used as hybridization probes to detect novel retroviruslike sequences. an 8.1-kilobase element with structural features of a retroviral provirus was isolated from a human genomic library by this approach. nucleotide sequence analysis of its 600-base-pair long terminal repeats revealed characteristic motifs known as regulatory signals for rna polymerase ii transcription: ccaat, tata, and attaaa. in addition, ... | 1987 | 2438428 |
| studies on the vibrio cholerae mucinase complex. ii. specific neuraminidase activity measured histochemically in a goblet cell assay. | the activity of neuraminidase prepared from the mucinase complex of vibrio cholerae was measured by a new, semi-quantitative goblet-cell assay. the counts of normal, alcianophilic, sialomucin-containing goblet cells (purple-stained) and neutral mucosubstance-containing goblet cells (magenta-stained) in serial sections of ileum were compared before and after neuraminidase treatment. the procedure provides a more natural assessment of the action of v. cholerae neuraminidase on the viscous intestin ... | 1987 | 2438408 |
| serological properties and processing in escherichia coli k12 of ompv fusion proteins of vibrio cholerae. | fusion proteins comprising the amino-terminal 99 amino acids of the bacteriophage ms2 replicase and various portions of ompv a major outer membrane protein of vibrio cholerae were expressed in escherichia coli k12. these fusions were expressed under the control of the pl promoter of bacteriophage lambda, and expression was controlled using a cits repressor. fusions occurring within the secretory signal sequence of ompv gave rise to the production of mature ompv. the efficiency, however, decrease ... | 1986 | 2436027 |
| sodium-channel inhibitors produced by enteropathogenic vibrio cholerae and aeromonas hydrophila. | 1987 | 2436014 | |
| avermectins: action on target pest species. | 2007 | 2435585 | |
| hybridocytochemistry with non-radioactive probes as a tool for biomedical research and cytodiagnostics. | the advent of dna and rna labelling techniques which allow non-autoradiographic detection of specific nucleic acid sequences has opened new possibilities for rapid diagnosis at the nucleic acid level. there are already practical applications. however, in order to fully compete with radioactive procedures an increase in sensitivity is still desired. | 2007 | 2435580 |
| the presence of the 30 nm filament structure of chromatins in intact chicken erythrocytes observed by 31p nmr. | a 31p nmr spectrum of chicken erythrocyte chromatins in vivo was obtained. the spectrum was characterized by examining the spectra of isolated nuclei and nondigested chromatins under various conditions. the results have shown that most chromatins in chicken erythrocyte nuclei assume the 30 nm filament structure as a second step of condensation of dna in vivo. | 1987 | 2435577 |
| [effect of kontrikal on the dynamics of proteolytic system indices in postischemic toxemia]. | it was shown in wistar male rats that the development of tourniquet shock was followed by an increase of proteolytic activity in the blood by 3 times, activity of aspartate aminotransferase (ast) by 3 times, that of alanine aminotransferase (alt) by 6 times, contents of urea and residual nitrogen by 2.5-3 times; level of alpha 1-protease inhibitor (alpha 1-pi) decreased by 4 times and that of alpha 2-macroglobulin (alpha 2mg) by 2.5 times. at administration of contrykal (10,000 u/kg) proteolytic ... | 1987 | 2435573 |
| [similarity of vibrio alginolyticus, v. cholerae and other vibrio species with respect to the structure of their flagellar apparatus and ribosomal 5s-rna]. | electron microscopic analysis of basal bodies of the flagella vibrio alginolyticus revealed a structure composed of four discs. the diameters of two discs localized in the cytoplasmic membrane appeared to be twice as little as those of the other two discs. in this respect the basal body of v. alginolyticus resembles that of v. cholerae. the 5s sequence of ribosomal rna from v. alginolyticus appeared to be similar to those of v. cholerae, v. harveyi and some other vibrios. comparison of 5s-rna se ... | 1987 | 2434145 |
| immunochemical properties of the lipopolysaccharide o-antigen of vibrio cholerae o1 in relation to its chemical structure. | d-glucuronic acid and d-glucosamine have an immunodominant role in the lipopolysaccharide (lps) o-antigen of both the ogawa and the inaba subtypes of vibrio cholerae o1. this was evident from the pronounced inhibitory effect on the lps precipitin reaction demonstrated by these monosaccharides and by oligosaccharides containing either of them which were isolated from lps hydrolysate. there was a considerable decrease in the antibody-combining capacity of chemically modified lps in which the carbo ... | 1986 | 2433386 |
| [genetic analysis of mutations affecting the synthesis of somatic o-antigen of vibrio cholerae]. | the mutations oagm and oagr affecting the synthesis of somatic o-antigen have been localized on the chromosome of vibrio cholerae el jor and classic biotypes by conjugational crosses between different donor and recipient strains. the mutations are localized in the vicinity of the arg marker in both classic and el tor biotypes of vibrio cholerae. | 1985 | 2432416 |
| chromatographic evaluation of the binding of haemoglobin to polyanionic polymers. | 2008 | 2432079 | |
| the surface charge of toxoplasma gondii: a cytochemical and electrophoretic study. | the surface charge of toxoplasma gondii tachyzoites was evaluated by means of binding of colloidal iron hydroxide particles at ph 1.8, cationized ferritin particles at ph 7.2 and ruthenium red to the cell surface, as visualized by electron microscopy and by direct measurements of the electrophoretic mobility (epm) of the cells suspended in solutions of different ionic strength and ph. at ph 7.2, t. gondii has a negative surface charge with a mean epm of--1.1272 +/- 0.0917 micron.s-1 x v-1 x cm. ... | 1986 | 2431157 |
| abnormalities of basophil "releasability" in atopic and asthmatic individuals. | 2008 | 2431023 | |
| [effects of enzymatic deglycosylation of human goiter thyroglobulin on its immunochemical properties]. | thyroglobulin (tg), isolated from soluble iodoproteins by ammonium sulphate fractionation, was enzymatically deglycosylated in vitro and analyzed by polyacrylamide gel electrophoresis, double immunodiffusion and non-commercial ria. carbohydrate and iodine content was chemically determined. by paage deglycosylated tg (dtg) showed the appearance of a major band in the 12s region and three slower migrating bands corresponding to higher aggregates than 19s tg. in immunodiffusion by testing native an ... | 1986 | 2430684 |
| effect of nutrient deprivation on lipid, carbohydrate, dna, rna, and protein levels in vibrio cholerae. | the response of vibrio cholerae to low nutrient levels was determined by measuring the concentrations of lipids, carbohydrates, dna, rna, and proteins over a 30-day starvation period. ultrastructural integrity was observed by transmission electron microscopy. total lipids and carbohydrates declined rapidly within the first 7 days, while dna and protein exhibited a more constant decline over the 30 days of starvation. in contrast, rna showed little decrease upon starvation. although neutral lipid ... | 1986 | 2430523 |
| t lymphocytes from hemophiliacs proliferate after exposure to factor viii product. | chronic exposure of hemophiliacs to allogeneic proteins via factor viii concentrate for control of bleeding could lead to a state of chronic antigen stimulation. immune function from eight hemophiliacs requiring large amounts of factor viii concentrate was assessed. it was found that 5 of 8 had a positive blastogenic response to low concentrations of factor viii in a 7-day thymidine uptake assay. separation of lymphocyte subpopulations indicated that the reactivity was contained in the t cell-en ... | 2008 | 2430365 |
| a ganglioside species (gd1 alpha) migrates at a slow rate and cmp-sialic acid severalfold faster in xenopus sciatic nerve: fluorographic demonstration. | the ninth dorsal root ganglion of adult xenopus laevis was labeled with n-acetyl-d-[6-3h]mannosamine, and intraaxonal migration of gangliosides was examined by analysis of the chloroform/methanol extract of each of 5-mm consecutive nerve segments by tlc coupled with fluorography. a unique disialoganglioside (gd1 alpha), which amounted to up to 83% of the total ganglioside in this nerve, migrated at 1-2 mm/day at 15 degrees c. this contrasts with the rapid transport of other ganglioside species p ... | 1986 | 2430059 |
| aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia. | we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ... | 1986 | 2430056 |
| aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia. | we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ... | 1986 | 2430056 |
| enhancement of histamine release from human basophils pretreated with different sialidases. | histamine release from human basophils was investigated in vitro after removal of cell membrane sialic acid by three different sialidases. pretreatment of the cells with sialidases from cl. perfringens, v. cholera or influenza virus a2 enhanced histamine release induced by subsequent stimulation of the cells with anti-ige or the plant lectin concanavalin a and caused a shift to the left of the dose-response curve for anti-ige. the enhanced histamine release was reflected in a increased calcium s ... | 1986 | 2429528 |
| [methodological aspects of the study of bacterial chemotaxis]. | 1986 | 2429040 | |
| structural studies of the o-antigen from vibrio cholerae o:21. | the o-antigen from vibrio cholerae o:21 has been investigated, using n.m.r. spectroscopy, methylation analysis, and smith degradation as the main methods. it is concluded that the o-antigen is composed of tetrasaccharide repeating-units having the following structure (in which hep = d-glycero-d-manno-heptose). (formula: see text). | 1986 | 2428496 |
| the effect of removal of d-fructose on the antigenicity of the lipopolysaccharide from a rough mutant of vibrio cholerae ogawa. | the lipopolysaccharide (lps) of a rough mutant (95r) of vibrio cholerae ogawa has been investigated chemically and serologically. d-fructose was released from lps under conditions (10mm trifluoroacetic acid, 60 degrees, 1 h) that liberated no other sugar constituent of the lps (2-amino-2-deoxy-d-glucose, d-glucose, l-glycero-d-manno-heptose). upon periodate oxidation, d-fructose and d-glucose were oxidised quantitatively, whereas approximately 50% of heptose was periodate-resistant. the data ind ... | 1986 | 2428494 |
| drosophila mutations that alter ionic conduction disrupt acquisition and retention of a conditioned odor avoidance response. | we have previously found that genetic alterations in neuronal ion channel function have specific effects on learning and memory of a conditioned courtship behavior in drosophila. using a behavioral assay of conditioned odor avoidance, we report here that shaker mutants, in which potassium-channel function is abnormal, are defective in both their rate and maximum level of acquisition when compared to wild-type flies. napts (no action potential, temperature sensitive) mutants, in which nerve excit ... | 1970 | 2427679 |
| the relationship between the keratocyst antigen (kca) and keratin. | the relationship of the keratocyst antigen (kca), the soluble component present in most keratocyst fluids, and keratin, was studied with immunofluorescence microscopy comparing their distribution in developing mouse embryonic teeth and in human ameloblastomas. in these tissues both molecules showed a strong codistribution in epithelial cells. in the embryonic teeth both molecules were present in the stratum intermedium cells between the stellate reticulum cells and ameloblasts, but the secretory ... | 1986 | 2427676 |
| oligodendroglia development in cell culture as monitored with a monoclonal antibody. | a new marker for young oligodendrocytes has been identified by a monoclonal antibody (mog-1, igm isotype) prepared from cerebellar plasma membrane stimulated mouse lymphocytes. mog-1 reactive cells in the mouse cerebellum first appear at day 19 of gestation. future white matter layers of fixed sections of neonatal rat cerebellum were labeled with mog-1. although em analysis has shown cell-surface binding by presumptive oligodendroglia in neonatal cerebellum, the antibody does not bind to compact ... | 1986 | 2427670 |
| comparison of the light-sensitive and cyclic gmp-sensitive conductances of the rod photoreceptor: noise characteristics. | patch-clamp recordings were made from outer segments of single rod photoreceptors isolated from toad retina. cyclic gmp (cgmp) and its hydrolysis-resistant analog, 8-bromo-cgmp, increased the conductance of excised membrane patches when applied to the intracellular face of the membrane but not when applied to the extracellular face. the rectification properties of the cgmp-dependent conductance depended on the concentration of divalent cations bathing the intra- and extracellular membrane faces. ... | 1970 | 2427668 |
| oncogenes in human primary hepatic cancer. | transfection assay of nih 3t3 cells was performed with dnas isolated from ten human phc (primary hepatic cancer) specimens and a hepatoma 7402 cell line. positive results were obtained in 7402 and in six out of ten phc dnas. human n-ras gene was identified in transfectants from 7402 dna and transformed cells from three phc dna samples, which had passed more than two cycles of transfection. the expression of n-ras was also remarkably enhanced in six out of nine poly(a)+rna samples isolated from p ... | 1986 | 2427530 |
| morphometric analysis of spermatozoa in the assessment of human male fertility. | a videomicrographic system was developed for measurement of morphometric parameters of human spermatozoa. contours of the images of spermatozoa on a video monitor are digitized by manually tracing them with the cursor of an electromagnetic digitizer integrated to a microcomputer. the accuracy and precision of the methodology were evaluated. a comparison of human sperm heads in shallow wet preparations and in dried, stained preparations indicated that the latter were smaller in length, width, pro ... | 1986 | 2427496 |
| building competence in delayed and disturbed preschoolers: outcome evaluation of an intensive day treatment program. | 2008 | 2427178 | |
| evidence that ruthenium red disturbs the synaptic transmission in the rat hippocampal slices through interacting with sialic acid residues. | ruthenium red (rr) at a concentration of 0.71 mm selectively blocked synaptic transmission in hippocampal slices. antidromically evoked potentials and fibre potentials were only little affected. the action of rr was reversible by washout, but only following shorter (40-50 min) times of incubation. after longer incubation times (hours), the abolished population spike did not recover after washout but could be restored by facilitation of the calcium transport into the nerve terminal with 3,4-diami ... | 1986 | 2427153 |
| identification and characterization of the nmyc gene product in human neuroblastoma cells by monoclonal antibodies with defined specificities. | increased n-myc (now designated nmyc in human gene nomenclature) gene expression has been detected at the transcriptional level in certain types of neoplasms. as yet, the n-myc gene product has not been identified. to detect and characterize the n-myc gene product, we have developed monoclonal antibodies against the putative n-myc gene product made in escherichia coli as a fusion protein. the antibodies that recognize the n-myc-specific regions were selected on the basis of their reactivities to ... | 1986 | 2426708 |
| molecular cloning and expression in escherichia coli k-12 of the o antigens of the inaba and ogawa serotypes of the vibrio cholerae o1 lipopolysaccharides and their potential for vaccine development. | the gene clusters that determine the biosynthesis of both the inaba and ogawa serotypes of the o antigen of the lipopolysaccharide of vibrio cholerae were cloned and expressed in escherichia coli k-12. restriction analysis of the clones demonstrated that about 15 kilobases were common to all clones and a further 5 kilobases were common to the ogawa clones. the o antigens expressed by e. coli k-12 had the specificity of v. cholerae. antibodies raised against e. coli k-12 that harbor one of these ... | 1986 | 2426198 |
| on the origin and progression of human breast cancer. | the mature human female breast contains thousands of hormone-sensitive, potentially milk-producing microorgans, called lobules. in the nonpregnant state they vary in size from 1 to 8 mm but most are 1 to 2 mm in diameter. each lobule is drained by a terminal duct attached to the main duct system. it is called the terminal ductal-lobular unit, which normally regresses at menopause. most breast diseases except papillomas in major ducts arise in terminal ductal-lobular units. disease processes such ... | 1986 | 2424309 |
| the interaction of myelin basic protein with tubulin and the inhibition of tubulin carboxypeptidase activity. | tubulin carboxypeptidase was found to be inhibited by myelin basic protein in a concentration dependent manner. the inhibition was produced by the interaction between myelin basic protein with the substrate. as a consequence of this interaction, turbid insoluble aggregates were formed at either 5 degrees or 37 degrees c. the turbidity increased by increasing the myelin basic protein concentration and it reached a plateau at a molar ratio of myelin basic protein to tubulin dimer of about 6. at pl ... | 1986 | 2423083 |