Publications

[chilean medicine during the government of president balmaceda].	this is a conference dictated on the occasion of the centennial of the first chilean medical congress, the foundation of the first public health council and the school of medicine of the university of chile (1889). during the government of president balmaceda, prominent chilean physicians and university professors were closely linked to the presidency, often in public office or congress. the conditions were then created for an accelerated development of many aspects of chilean medicine, includin ...	2008	2519385
[asymptomatic cholelithiasis. when is a cholecystectomy justified?].	since the introduction of routine abdominal echography, silent gallstone has become a major medical concern. the management of a patient with silent gallstone, is very controversed, and routine cholecystectomy is not always beneficial. aged and high operative risk patients, should be treated conservatively. literature review can identify subgroups of patients in whom cholecystectomy is justified: patients with micro or macro-lithiasis, patients in whom oral cholecystography reveals multiple floa ...	2008	2519334
[a cytochemical study of lysosomal system in rat junctional epithelium after intravenous horseradish peroxidase injection].	lysosome formation was studied in rat junctional epithelium (je) by detection of acid phosphatase (acpase) using the cerium reaction method after intravenous injection of horseradish peroxidase (hrp). intravenously injected hrp was endocytosed by the whole je, especially at the coronal portion. acpase activity was also observed in numerous organellae (vacuoles, dense bodies, multivesicular bodies and golgi cisternae) in the junctional epithelial cells. these acpase positive organellae often fuse ...	1989	2519301
decreased level of immunoreactive epidermal growth factor in the hindlimb muscle of muscular dystrophic mice.		2008	2519267
[request for a hemogram as a marker of the quality of health care].	the evaluation of markers of the health care activity, such as the reason for requesting a peripheral blood count in everyday practice and its inclusion in the clinical record, constitutes an estimate of the quality of care. to this end, we have reviewed the requests of 160 peripheral blood counts in our center during one month. it was found that the request itself and/or its cause had not been mentioned in the clinical record in 32.5% of instances. in the cases where the indication could be asc ...	2007	2518967
[evaluation of results of school health examination in the murcia region. 1986/87].	in the present study, we have carried out an evaluation of school examinations in 47,943 children in the past course. we have compared our results with those from similar previous studies in other parts of spain. our findings were comparable in visual acuity and scoliosis, and not so much about cryptorchidism and phimosis. we think that examinations are necessary, but it is needed to increase the quality and the effectiveness, to get closer to the knowledge of usefulness and to the correct evalu ...	2008	2518932
[undergraduate medical education in cantabria and its relevance to the new health system].	considering that medical schools should serve the community to which they provide professionals and that, therefore, they should have a knowledge of the community health needs, we evaluated the adequacy of the teaching program of the medical school of cantabria and the attitudes and expectations of the medical students. the results showed a majority of students younger than age 20 (90.2%), with a female predominance (68.5%), from upper and middle classes and with professional expectations of bec ...	2008	2518929
[impact of the development of a rural health center on the demand for emergency care].	the new legislation on primary health care (phc) has reorganized the implementation of services. we have evaluated the impact on the demand of emergency attention in the rural town of almodóvar del pinar (cuenca); this attention was previously given by the apd physician and now partially depends from the health center. overall 97 cases were assisted, as compared with 229 in the preceding period (p less than 0.001). the motives for consultation remained unchanged, as well as the timing of the dem ...	2007	2518912
[immunoenzymatic determination of free immunoglobulin light chains in the cerebrospinal fluid: its value in the biological diagnosis of multiple sclerosis].	based on previous data on free immunoglobulin light chains in the cerebrospinal fluid (csf) of patients with multiple sclerosis (ms), we evaluated free kappa and lambda light chains in the csf from 3 patients groups: (a) 42 with ms, (b) 16 with other neurological inflammatory diseases (onid) and (c) 42 with non inflammatory neurological diseases (nind) used as control. the kappa and lambda light chains contents were evaluated using a specific and sensitive (0.5 mcg/ml) enzyme linked immunosorben ...	2008	2518740
furazolidone-induced interstrand cross-links in vibrio cholerae dna. study of conformational change by circular dichroism.	vibrio cholerae dna bearing furazolidone-induced interstand cross-links show a change in the characteristic circular dichroism spectra of the dna itself in dilute buffer. the change in c.d. spectra was characterized by a shift of the positive band around 272 nm to lower wavelength and a loss of ellipticity of the negative band around 242 nm, and is similar to that exhibited by mitomycin linked vibrio cholerae dna under identical conditions and is suggestive of a conformational change of dna bear ...	1989	2518733
[in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics].	in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ...	1989	2518646
survival and growth of food poisoning bacteria following inoculation into cottage cheese varieties.	following inoculation into cottage cheese varieties with and without sorbic acid, obtained directly from the manufacturer, strains of enteropathogenic escherichia coli and other e. coli survived but failed to multiply during storage at 7, 10 or 25 degrees c. in the absence of sorbic acid spoilage due to pseudomonas fluorescens occurred after storage for 5-13 days at 7 or 10 degrees c and 1-2 days at 25 degrees c. salmonella enteritidis, s. hadar, s. saint-paul, s. typhimurium and s. virchow surv ...	1989	2518228
[costs of caries therapy and prevention in a school].	the prevalence of dental caries in the school children population in rieti is very high, according to low levels of fluoride content in drinkable water, similar to those detected in other areas of italy and europe. the aim of our research is to propose a combined prevention protocol, utilizing fluoride tablets and dental therapy through structures already existing in the public health national organization. the application of this combined (profilaxis-therapy) protocol is expected to decrease th ...	2006	2517848
relationship between viral hepatitis and four trace elements in sera.		1989	2517616
[quantitation of human serum apolipoprotein cii by radial immunodiffusion assay].	a radial immunodiffusion assay (rid) for quantitation of human serum apolipoprotein cii (apocii) was developed. antiserum to apocii was raised in goats with purified apocii. the antibody-gel plate was prepared with 1% agarose. 5 microliters of each sample or standard serum was added in a well. diffusion was performed at 25 degrees c or 37 degrees c for 48 h. the standard curve with a working range of 0.8-4.5 mg/dl was plotted. the minimum measurable concentration of apocii was 40 ng. the intra-a ...	1989	2517121
the discovery of the enzyme test for rh antibodies.		1989	2515654
the estimation of dietary protein intake in chronic renal failure.	forty-five adult clinic patients with chronic renal failure each supplied a 4-day weighed dietary record, a 24-h urine collection, and a nocturnal spot urine sample. total nitrogen (n) losses derived from the urines were corrected for proteinuria and non-urea nitrogen excretion. individual estimates of n intake were compared by correlation and assessing the level of agreement. daily urea n excretion derived from the spot sample correlated well with the 24-h collection p less than 0.001, but the ...	1989	2515492
sustained high-dose nitroglycerin transcutaneous patch therapy in angina pectoris: evidence for attenuation of effect over time.	the safety and efficacy of using continuous high-dose transcutaneous nitroglycerin in doses up to 100 mg/24 hours in chronic stable angina was assessed in 20 patients using serial treadmill testing. patients had first to show a response to sublingual nitroglycerin with a 20% improvement in exercise time. all patients were then titrated with 20 mg (40 cm2), 60 mg (120 cm2), 80 mg (160 cm2) or 100 mg (200 cm2) patches, until intolerable headache in association with a 10 mmhg reduction in blood pre ...	2008	2515215
time of insemination and its effect on in-vitro fertilization, cleavage and pregnancy rates in gnrh agonist/hmg-stimulated cycles.	in this prospective study, we compared the effect of delayed inseminations on fertilization, cleavage and pregnancy rates in two groups of patients. in one group, the ovarian stimulation was performed with a clomiphene citrate/human menopausal gonadotrophin/human chorionic gonadotrophin (cc/hmg/hcg) protocol. the other group was pre-treated with gonadotrophin-releasing hormone agonist (gnrha) and ovarian stimulation was carried out with an hmg/hcg protocol. under both protocols, a delay of 2, 4 ...	1989	2515208
an immunocytochemical study of the proliferating cell nuclear matrix antigen p125/6.5 during rat spermatogenesis.	in previous studies of proliferating mammalian cells a p125/6.5 nuclear matrix antigen displaying a marked increase in mitotic cells has been identified. this antigen was investigated by immunocytochemistry of cryosections of testes at different stages of postnatal development: newborn, 20 days after birth and sexually mature rats. in sertoli cells, the distribution of the p125/6.5 antigen parallels [3h]thymidine incorporation: present in newborn and absent in sexually mature testes. the p125/6. ...	1989	2515195
an outbreak of nosocomial cholera in a 755-bed hospital.	from 30 october to 7 december 1984, an outbreak of nosocomial cholera involving 11 cases of biotype el tor, serotype inaba, took place in a 755-bed hospital in southern thailand. the outbreak occurred primarily among patients admitted with severe illness. of the 11 cases, 7 were children and 4 were adults. most cases had mild symptoms of cholera and no case died in this outbreak. the first 2 cases occurred sporadically with a subsequent cluster of cases showing an explosive pattern. a case-contr ...	1989	2514477
[increased efficiency in the disinfection of sewage by lactic acid bacteria].	the antimicrobic properties have been studied in 30 strains of lactobacilli. as a result a strain, the strongest antagonist relative to choleric vibrios and other enteropathogenic microorganisms, is selected. lactobacilli are found to retain their viability and biological activity in the activated sludge during the whole period of observation (6 months). biological disinfection of sewage is shown possible to be intensified using the activated sludge inoculated by the culture of the selected lact ...	1989	2514340
tongue flaps in repair of large palatal defects.	anteriorly based dorsal tongue flaps were used in 19 cleft patients for closing large palatal defects. the procedure was successful in 17 patients. one patient had partial marginal necrosis after division of the pedicle, while another had complete necrosis of the distal part of the flap. our results support the view that for success of the procedure, adequate length, width and thickness of the flap are important, while the height of the palate also plays a role.	2008	2509583
assay of heat-labile enterotoxins by their adp-ribosyltransferase activities.	simple enzymatic assays to detect heat-labile enterotoxins whose modes of action are similar to that of cholera toxin were evaluated. the assays are performed by using an artificial substrate, diethylamino benzylidine-aminoguanidine, which is an adp-ribose acceptor. the product, formed in the presence of nad+, can be quantitated by spectrofluorometric, spectrophotometric, or high-performance liquid chromatographic (hplc) methods. as little as 25 ng (spectrofluorometry) or 125 ng (spectrophotomet ...	1989	2509510
the immunodiagnosis of schistosoma mansoni infection in the different clinico-parasitological stages.	a total of 52 bilharzial mansoni patients were examined, 20 patients with early intestinal infection with living ova in stools, 20 patients with hepatosplenomegaly but without ascites, also with viable ova in stools, and 12 patients with hepatosplenomegaly and ascites without ova in stools; but in the rectal snip. also 20 normal persons were examined. an idt test was performed by s. mansoni adult worm antigen. s. mansoni adult worm and egg antigen extracts were used for performing ciep, ihat and ...	1989	2504828
oral rehydration formula containing alanine and glucose for treatment of diarrhoea: a controlled trial.	to determine whether adding l-alanine to the glucose based oral rehydration solution recommended by the world health organisation would improve its efficacy in treating acute diarrhoea.	1989	2502251
effects of salinity on the survival of non-o1 vibrio cholerae under environments of low temperature.	under low temperature conditions, the effects of survival and growth of non-o1 vibrio cholerae were studied in laboratory microcosms. as the environmental factors, various concentrations of nacl solution and that of mono- and bivalent cation added nutrient of polypeptone were used under several temperatures. the results of these experiments suggested an extended survival of non-o1 v. cholerae at low temperature. when a range of nacl concentration in microcosms water was between 0.4% and 2.0% wit ...	1989	2501431
"new" food-borne pathogens of public health significance.	recent work by epidemiologists and microbiologists has uncovered several hitherto unrecognized food-borne bacterial pathogens of public health significance. one of these, listeria monocytogenes, has attracted considerable attention because of two major cheese-related outbreaks of listeriosis that were characterized by cases of meningitis, abortion, and perinatal septicemia. thus far, l. monocytogenes has been responsible for well over 300 reported cases of food-borne listeriosis, including about ...	1989	2501376
[soy lipoxygenase as the object for the primary testing of potential inhibitors of leukotriene biosynthesis].	the comparative study of the ability of three compounds (indomethacin, agent bw 755 c and arachidonate hydroxamic acid) to inhibit the activity of preparations of soya bean lipoxygenase and to block leukotriene c4 biosynthesis in human leukocytes recorded by reversed phase liquid chromatography was performed. it was shown that irrespectively of purification degree soya bean lipoxygenase is a sufficiently adequate object for primary testing of inhibitors of biosynthesis of leukotrienes with the g ...	1989	2501105
valproate and spina bifida.		2008	2500206
profile of the gmc. 1978 and all that.		2008	2500205
hearing voices.		2008	2496851
bma's doubts on aids care in reformed nhs.		2008	2496849
case for diagnosis. chronic compensated hemolytic anemia.		2008	2494590
boxing and the brain.		2008	2493277
purification of high and low molecular weight plasminogen activator inhibitor 1 from fibrosarcoma cell-line ht 1080 conditioned medium.	functionally active (high-mr) and inactive (low-mr) plasminogen activator inhibitor 1 (pai) have been purified from fibrosarcoma cell-line ht 1080 conditioned medium, containing 1% fetal calf serum. the two forms were first purified by affinity chromatography on heparin-sepharose and then separated from each other by gel filtration on sephadex g-150. the final purification was achieved by affinity chromatography on insolubilized monoclonal antibodies towards human pai. alternatively, the low-mr ...	1989	2493266
'dental sedation. a review'.		2008	2493260
inhibition of ca2+-induced cytosolic enzyme efflux from skeletal muscle by vitamin e and related compounds.	1. efflux of an intracellular enzyme (creatine kinase) from normal rat skeletal muscles was induced by treatment with the ca2+ ionophore a23187. addition of alpha-tocopherol (230 microm) to the incubation medium was found to significantly diminish this efflux, and this effect was mimicked by alpha-tocopherol acetate, phytol and isophytol, but not by trolox c (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid). 2. analysis of muscle cation content has shown that these protective effects of a ...	1989	2493242
analysis of amikacin-resistant pseudomonas aeruginosa developing in patients receiving amikacin.	during a 36-month period, 28 patients treated for infections due to amikacin-susceptible pseudomonas aeruginosa subsequently developed infections or colonization with amikacin-resistant p aeruginosa at the same site. eleven amikacin-susceptible/-resistant pairs of isolates were analyzed for aminoglycoside-inactivating enzymes, plasmid profiles, cellular proteins, outer membrane proteins (omps), lipopolysaccharide (lps) profiles, and amikacin uptake. while clearly distinct from isolates of other ...	1989	2493230
structural studies of the asparagine-linked sugar chains of two immunoglobulin m's purified from a patient with waldenström's macroglobulinemia.	the structures of the sugar chains present in two human monoclonal igm molecules purified from the serum of a patient with waldenström's macroglobulinemia have been determined. the asparagine-linked sugar chains were liberated as oligosaccharides by hydrazinolysis and labeled by reduction with nab3h4 after n-acetylation. their structures were studied by serial lectin column chromatography and sequential exoglycosidase digestion in combination with methylation analysis. these two igm's were shown ...	1989	2493215
in vitro susceptibility of feline herpesvirus-1 to vidarabine, idoxuridine, trifluridine, acyclovir, or bromovinyldeoxyuridine.	in vitro activities of 9-[( 2-hydroxyethoxy] methyl) guanine (acyclovir), (e)-5-(2-bromovinyl)-2'deoxyuridine, 9-beta-d-arabinofuranosyladenine (vidarabine), 5-iodo-2'-deoxyuridine (idoxuridine), and 5-trifluoromethyl-2'-deoxyuridine (trifluridine) were studied against 6 strains of feline herpesvirus-1. a significant difference was not detected among viral strains in their susceptibility to these compounds (p = 0.442). the relative potency of these compounds was trifluridine much greater than id ...	1989	2493210
monitoring of intestinal transplant rejection.	our results show that maltase, sucrase, and lactase activity are present at a normal level in nonrejecting small bowel transplants after an initial postoperative decline. this confirms that the disaccharide absorbing capacity of these grafts is intact. in allogeneic bowel, however, the levels of maltase and sucrase decline as histologic rejection occurs. these results suggest that serial maltase, sucrase, and possibly lactase levels in allogeneic intestinal transplants may serve as a useful adju ...	1989	2493205
experimental transmission of crimean-congo hemorrhagic fever virus by hyalomma truncatum koch.	larval hyalomma truncatum ticks were infected with crimean-congo hemorrhagic fever (cchf) virus by allowing them to engorge on viremic newborn mice. the overall tick infection rate was 4.4% (24/542). virus was detected in specimens for greater than or equal to 160 days postinfection. transstadial transmission to the adult tick stage was observed and horizontal transmission to a mammalian host was demonstrated. horizontal transmission of cchf virus to uninfected adult ticks occurred while feeding ...	1989	2493203
genetics and fetal hydantoin syndrome.		2008	2493186
[teratogenic effect of thiotepa].		2008	2493184
a review of experimental studies of iodine deficiency during fetal development.	iodine deficiency is now recognized as a major international public health problem. it is estimated that 800 million people may be at risk of the effects of iodine deficiency. in humans, the effects occur at all stages of development: the fetus, the neonate, the child and adult. the effects are now denoted by the term iodine deficiency disorders (idd). they include miscarriages, stillbirths, congenital anomalies, as well as the more familiar goiter, cretinism, impaired brain function, and hypoth ...	1989	2493080
serum fatty acid profile of elderly tube-fed men in a nursing home.	severely dependent tube-fed elderly men with dementia in this va nursing home are nourished exclusively with isocal, which provides only four fatty acids in amounts over 1% of the total (palmitic, oleic, linoleic, and alpha-linolenic). it has not been proven that the frail elderly can efficiently convert linoleic to arachidonic acid, and alpha-linolenic to eicosapentanoic and docosahexaenoic acids. therefore, we compared the serum profile of fatty acids in six of the tube-fed elderly men with th ...	2008	2493045
lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats.		1989	2492809
analysis of cytotoxic t cell responses to intracameral allogeneic tumors.	injection of p815 mastocytoma tumor cells into the anterior chamber (ac) of balb/c mice results in progressively growing intraocular tumors, whereas injection of the same dose of tumor cells subcutaneously results in rapid rejection of the tumor. previous results indicated that ac p815 inoculation results in an unorthodox immune response characterized by suppressed delayed hypersensitivity, elevated antibody levels and priming for cytotoxic t lymphocyte (ctl) activity. since mice inoculated in t ...	2008	2492486
immunobiological relationships among new cholera toxins produced by ct gene-negative strains of vibrio cholerae o1.	the enterotoxic activities of partially purified new cholera toxins (nct) prepared from the ct gene-negative strains of vibrio cholerae serotype o1 isolated from cases of diarrhoea in man and from diverse environmental sources were assayed in the rabbit ileal-loop model and the toxin unit was calculated in micrograms of protein content. the secretory activities of one unit of homologous and heterologous enterotoxins were completely neutralised by 2.5 units of crude antiserum raised against one n ...	1989	2492345
recombinant system for overexpression of cholera toxin b subunit in vibrio cholerae as a basis for vaccine development.	we have constructed an overexpression system in which the gene encoding the b subunit of cholera toxin (ctb) was placed under the control of the strong tacp promoter in a wide host range plasmid. recombinant nontoxigenic classical and e1 tor vibrio cholerae strains of different serotypes harboring this plasmid excreted 10- to 100-fold higher amounts of ctb than any other wild-type or recombinant strain tested and may therefore be useful killed oral vaccine strains. the manipulations to place the ...	1989	2492108
toxigenic vibrio cholerae o1 infection acquired in colorado.		1989	2491910
immunocytochemical study of localization and traffic of thyroid peroxidase/microsomal antigen.	we studied the distribution of binding sites for anti-peroxidase monoclonal antibody and anti-microsomal antibodies on isolated human thyroid follicles and a human thyroid cell line. both open follicles and cells were incubated first with antibodies at +4 degrees c, then with colloidal gold labelled protein a. the topography of the binding sites for monoclonal anti-peroxidase antibody corresponded closely to the expected cell surface distribution of endogenous thyroid peroxidase since labelling ...	1989	2491623
detection of marker associations with a dominant disease gene in genetically complex and heterogeneous diseases.	linkage disequilibrium of a marker allele with disease may characterize a chromosomal region containing the disease gene. in several diseases, only a limited number of pedigrees are linked to a particular region, because of linkage heterogeneity. disequilibrium in this situation is more easily detected when the association is positive (an infrequent marker allele associated with disease mutation), and sampling is conditional on presence or absence of illness in individuals or gametes. defining h ...	1987	2491015
a bacteriological study of cholera in gulbarga district.		1989	2486988
enzyme-linked immunosorbent assays.		1994	2486571
[the fate of pathogens in waste-waters].	a large variety of pathogenic organisms capable of transmission by the faecal-oral route may be found in wastewaters. among the bacteria salmonella, shigella, vibrio cholerae, yersinia and campylobacter are the important agents of concern. also the human enteric viruses (poliovirus, coxsackievirus, echovirus, hepatitis a virus, rotavirus) have been shown to be present in domestic waste and may not be completely removed by conventional sewage treatment processes, including chlorination. discharge ...	1989	2484470
[current problems in the microbiological control of food].	the paper deals with a number of aspects of current interest and significance on the subject of the microbiological testing of food with particular reference to the role of research institutes. the main objectives of microbiological testing of food are essentially two: (i) to establish the absence of a human health hazard due to microbial contamination of food (food safety); and (ii) to define the quality standard of food (food quality). as far as the first of these objectives is concerned, the ...	1989	2483651
6-nitrobenzo[a]pyrene.		2008	2483416
occurrence and characteristics of agglutination of vibrio cholerae by serum from the eastern oyster, crassostrea virginica.	cell-free hemolymph (serum) of the eastern oyster, crassostrea virginica, agglutinated vibrio cholerae, including all o1 serovars and biovars. seventy-nine other strains of bacteria, including 14 genera and 26 species, were not agglutinated. the a, b, and c factors of o1 antigen were not involved in agglutination. bacterial agglutinating (ba) activity was demonstrated for oysters inhabiting different environments of the u.s. atlantic and gulf coasts. oyster serum ba titers showed high individual ...	1989	2483041
variation in epitopes of the b subunit of el tor and classical biotype vibrio cholerae o1 cholera toxin.	variation in epitopes of the b subunit of cholera toxin (ct-b) produced by strains of el tor and classical biotype vibrio cholerae o1 was examined using monoclonal antibodies prepared to v. cholerae 569b ct. ct-b epitopes were markedly conserved for v. cholerae classical biotypes. in contrast, epitope variation was observed for el tor biotypes, which produced both a classical-like ct-b and a unique ct-b lacking at least one epitope common to 569b ct-b. the missing epitope was located outside the ...	1989	2482860
relationship between structure and antigenicity of o1 vibrio cholerae lipopolysaccharides.	the relationship between the release of fructose from o1 vibrio cholerae lipopolysaccharides (lps) by dilute acetic acid hydrolysis and the decrease in their antigenicity was examined. decrease in the antigenicity of lps was not parallel with the release of fructose, and occurred very much later than the latter. periodate oxidation of lps resulted in the total elimination of the fructose and glucose, and two-thirds of the heptose constituents, but no difference in the antigenicity of lps was obs ...	1989	2482336
separation of branched sialylated oligosaccharides using high-ph anion-exchange chromatography with pulsed amperometric detection.	ten characterized sialylated oligosaccharides from bovine fetuin (b. bendiak, m. harris-brandts, s. w. michnick, j. p. carver, and d. a. cumming, biochemistry, in press; and d. a. cumming, c. g. hellerqvist, m. harris-brandts, s. w. michnick, j. p. carver, and b. bendiak, biochemistry, in press) were chromatographed using high-performance anion-exchange chromatography with pulsed amperometric detection. at near neutral ph values, oligosaccharides were separated according to their number of forma ...	1989	2481411
[changes in cerebral level of monoamines by japanese encephalitis virus infection].	the changes in monoamine levels of different brain regions following japanese encephalitis virus (jev) intraperitoneal inoculation were examined in experimentally jev-infected mice. in addition, virus distribution was studied using infectivity assay and immuno-histochemistry of viral antigen. 1) the level of monoamines in brain tissues was not affected by 48 hours after viral inoculation, but marked effects were elicited at 96 hours after the inoculation. the cerebral concentration of 5-hydroxyi ...	1989	2481370
prostatic "dysplasia/atypical hyperplasia". terminology, histopathology, pathobiology, and significance.		2008	2481361
[cytogenetic and cytological approaches to detection of gamete abnormalities (review of the literature)].		1989	2481072
neovascularization of surface demineralized dentin.	uneventful healing of the wound site created by periodontal reconstructive surgery is crucial for the long term survival of the dentition. wound healing has been shown to be initiated and mediated by matrix components and polypeptide growth factors. neovascularization (or angiogenesis) is one of the most important events in the healing process of a wound site. any increase in the degree and/or rate of neovascularization could result in more rapid or complete healing. previously, we have shown th ...	1989	2481025
differentiation and mechanisms of prevention and termination of verapamil-sensitive sustained ventricular tachycardia.	the purpose of this study was to differentiate by means of electrophysiologic study, a drug's ability to terminate or to prevent ventricular tachycardia (vt). differences between the 2 effects were examined in patients with vt and the underlying mechanisms were studied in verapamil-responsive idiopathic sustained vt. the clinical significance of the distinction for chronic oral drug therapy is discussed. thirty-five cases of inducible sustained vt were studied. a drug was considered "preventive" ...	1972	2480708
who benefits most from respite care?	this study examines the effect of five measures of utilization of respite care services (desire to use respite care services, actual use, accessibility, scheduling and helpfulness) upon mothers' coping resources. using the analysis of variance between utilization measures and coping resources with the following intervening variables as covariates--mothers' self-esteem, family cohesion and adaptation, and clients' and mothers' characteristics--it was found that respite care acts as a differential ...	2008	2479611
[effect of interferon on the absorption of staphylococci by the macrophages of mouse peritoneal exudate].		1985	2477669
influence of dietary hexachlorocyclohexane isomers on lipid metabolism in albino rats.	dietary intake of beta- and gamma-hexachlorocyclohexane (beta- and gamma-hch) by albino rats for two weeks (at 800 ppm level) resulted in impairment of lipid metabolism, viz. hyperlipemia and fatty metamorphosis of liver. liver fat content was increased by both beta- and gamma-hch. significant increases were observed in triglyceride and phospholipid fractions of blood in these experimental animals. the incorporation of [14c]acetate and palmitate into liver and blood lipids was higher in hch pret ...	1989	2476378
red krypton and blue-green argon laser diabetic panretinal photocoagulation.	eyes with three or four diabetic retinopathy risk factors received laser panretinal photocoagulation with random selection of either blue-green argon (42 eyes) or red krypton (40 eyes) laser to determine if one laser was superior to the other. after 6 months, visual acuity preservation or improvement was obtained in 33 (79%) argon- and 34 (84%) krypton-treated eyes. peripheral iv-4e visual field constriction of 7% occurred with argon and 10% with krypton. vitreous hemorrhaging after treatment oc ...	1967	2476366
the three-dimensional structure of the zona pellucida in growing and atretic ovarian follicles of the mouse. scanning and transmission electron-microscopic observations using ruthenium red and detergents.	the present study provides further details on the fine-structural three-dimensional architecture of the zona pellucida (zp) in growing and atretic follicles of mice by use of ruthenium red in combination with the detergents triton x100 and saponin. these detergents were used for extraction of the "soluble" fraction of the zonal proteins in an attempt to expose the "structural" zonal glycoproteins, which in turn can be viewed as minute three-dimensional networks upon transmission- and scanning el ...	1989	2476232
["tertiary" lymphedema--procedures in lymph drainage therapy].	in patients with malignant tumours (with or without secondary lymphedema) a so-called tertiary lymphedema may develop. this edema is mostly caused by exacerbation of the primary tumor, by local recurrences or by loco-regional metastases. if a tertiary lymphedema is suspected oncologic diagnostic investigations must be immediately performed. during this period lymphatic drainage therapy is stopped. in patients with safely established hopeless prognosis drainage therapy may be continued if necessi ...	1966	2475986
exocrine pancreatic response to isoproterenol in the rabbit under secretin and cck stimulation.	the effect of isoproterenol (0.3 micrograms/kg/min) on exocrine pancreatic secretion stimulated by secretin (1. c.u./kg/h) and the octapeptide of cholecystokinin (op-cck 0.15 ivy dog units/kg/h) was studied in anaesthetized rabbits. pancreatic blood flow remained unaffected by the action of isoproterenol; however, the administration of the beta-adrenergic agonist over an infusion of secretion an op-cck led to an increase in pancreatic juice flow (approximately 146% for secretin and 125% for op-c ...	1989	2475982
properties of acetylcholine-receptor activation in human duchenne muscular dystrophy myotubes.	in human myotubes cultured from biopsies of normal subjects and dystrophic patients we investigated, with the patch-clamp technique, the activation properties of the nicotinic acetylcholine receptor (achor) in the presence of acetylcholine and suberyldicholine. the single-channel conductance and the lifetime of the openings were not found to differ. in contrast, the average frequency of openings was about four times higher in duchenne muscular dystrophy (dmd) myotubes in the presence of equal am ...	1989	2475875
[the clinical value of determining the general antitrypsin activity of the blood and exudate in tuberculous pleurisy].	determination of antitryptic activity of blood serum and exudate in patients with tuberculous pleurisy revealed that concentrations of trypsin inhibitors were significantly increased while their activity was markedly decreased (2-3-fold) in the exudate. this indicated exhaustion of the adaptation mechanisms in the inflammatory areas, which required their local correction. intrapleural administration of contrical increased the body's protective and adaptation potentials and treatment efficiency s ...	1965	2475869
[whipple's disease].		2008	2475663
cytochemical analysis of dna organization during encapsidation in herpes simplex virus.	the organization of intranuclear herpes simplex virus dna in rabbit fibroblast cells infected for 7 hr with hsv type 1 was examined before and during encapsidation by electron microscopic cytochemistry. most non-encapsidated viral deoxyribonucleoprotein fibers exhibited a non-nucleosomal configuration. empty capsids within the virus-specific regions of infected nuclei were wrapped with portions of the viral genome which adhered tightly to their surfaces even under conditions that loosened and sp ...	1989	2475542
[a method of fixation and disinfection of microbial cells on magnetic sorbents].	the 96% ethanol has been found fairly effective for the fixation and disinfection of yersinia pestis, vibrio cholerae, pseudomonas mallei, and pseudomonas pseudomallei after their contact with magnetic polyacrylamide sorbents containing specific immunoglobulins for ligands. such step is recommended for the quantitative immunofluorescence test employed for rapid analysis, diagnosis, and identification of the above microorganisms.	1989	2474712
[enzyme activity of induced auxotrophic mutants of el tor vibrio cholerae].	vibrio cholerae induced auxotrophic variants nonfermenting several carbohydrates simultaneously have been obtained for the first time. eltor vibrio clones have been selected, referred to heuberg's groups v and vi by their enzymic activities. the findings of the present study evidence that, when isolating eltor vibrio strains negative towards differential carbohydrates, in case of a positive agglutination test, the incubation period should be prolonged to 3 days. the principal differential diagno ...	1989	2474711
cd2 expression and function in lepromatous leprosy.	leprosy is a spectral disease in which clinical presentation is thought to be related to the host immune response. previous investigations have suggested that selective unresponsiveness to mycobacterium leprae in patients with lepromatous leprosy is due to the presence of m. leprae-specific t-suppressor cells. however, it has recently been suggested that cd2 modulation was the mechanism for the observed impaired immune response in lepromatous patients. therefore, we studied the expression of cd2 ...	1989	2474503
gene-specific labeling of chromatin for electron microscopy.	in restriction enzyme digested xenopus laevis erythrocyte chromatin, the 5s oocyte genes were specifically labeled with colloidal gold so they could be identified in an electron microscopic study. nuclei were digested first with hindiii restriction endonuclease followed by t7 exonuclease to leave single-stranded 3' tails on the fragments. a biotinylated 15-mer oligonucleotide complementary to the tail on the 3' end of the 5s gene was annealed to the chromatin, and streptavidin complexed colloida ...	1989	2474479
influenza a virus enhances staphylococcus aureus-induced basophil histamine release in normal individuals and patients with intrinsic asthma.	influenza a virus was found to enhance staphylococcus aureus-induced histamine release in human leukocyte suspensions, but did not per se release histamine. this potentiating effect was found to be similar in cells from normal individuals and from patients with intrinsic asthma. the enhancement of mediator release could be due to viral neuraminidase on the surface of the virus, since a similar potentiating effect was caused by a purified neuraminidase preparation obtained from vibrio cholerae, a ...	1989	2470268
influenza a virus enhances allergic histamine release. effect of neuraminidase.	histamine release was examined in leukocyte suspensions from patients allergic to house dust mite, grass pollen, birch pollen or cat dander. influenza a virus was found to enhance the antigen-induced mediator release, but did not cause release of histamine from the cells per se. also histamine release induced by anti-ige in cell suspensions from normal individuals was enhanced by virus. the potentiating effect of influenza a virus might be due to neuraminidase on the surface of virus, since a si ...	1989	2470267
[use of gnotobiotic animals for the study of the pathogenesis of cholera intoxication].	germ-free suckling rabbits and minipigs can be recommended as models suitable for the study of different aspects of the pathogenesis of cholera intoxication. in minipigs, individual representatives of intestinal autochthonous microflora produce different effect on the sensitivity of the animals to the toxigenic and choleragenic action of vibrio cholerae antigen introduced by oral administration, that should also be taken into consideration in the determination of residual toxicity during the tri ...	1988	2469271
simple, rapid method for direct isolation of nucleic acids from aquatic environments.	direct isolation of nucleic acids from the environment may be useful in several respects, including the estimation of total biomass, detection of specific organisms and genes, estimations of species diversity, and cloning applications. we have developed a method that facilitates the concentration of microorganisms from aquatic samples and the extraction of their nucleic acids. natural water samples of 350 to greater than 1,000 ml are concentrated on a single cylindrical filter membrane (type svg ...	1989	2467621
[tracheal agenesis: which surgical measures are sensible?].	tracheal agenesis is a rare congenital malformation. up to now no surgical concept exists for a definitive correction. in this paper we describe our experiences with an own case and discuss the purpose and justification of temporary surgical measures.	2007	2467457
[tracheal agenesis: which surgical measures are sensible?].	tracheal agenesis is a rare congenital malformation. up to now no surgical concept exists for a definitive correction. in this paper we describe our experiences with an own case and discuss the purpose and justification of temporary surgical measures.	2007	2467457
[molecular biology of keratins and their significance in the clinical diagnosis of tumors].	keratins are the most heterogenic class of intermediary filament molecules and form tonofilaments in all types of epithelial cells. at least 19 different human keratins have been demonstrated. on the basis of biochemical and immunological criteria, these may be subdivided into two subtypes (type i and type ii). recent molecular biological investigations have provided considerable insight into the construction of the keratin molecule and the tonofilament structure and the corresponding gene struc ...	1989	2467415
comparison of hemolysins of vibrio cholerae non-o1 and vibrio hollisae with thermostable direct hemolysin of vibrio parahaemolyticus.	hemolysin (vh-rtdh) produced by vibrio hollisae and hemolysin (nag-rtdh) produced by vibrio cholerae non-o1 were characterized and compared with hemolysin (vp-tdh) produced by vibrio parahaemolyticus. these three hemolysins are each composed of two subunits and have similar, but not identical, molecular weights. the amino acid compositions of vp-tdh and nag-rtdh are similar, but are different from that of vh-rtdh. the three hemolysins showed similar lethal toxicities to mice. the effects of temp ...	1988	2466545
infection and immunity to vibrio cholerae, salmonella typhimurium and escherichia coli in a rabbit model.	cholera disease can be induced in the rabbit by duodenal inoculation (di) of vibrio cholerae organisms after ligation of the cecum (c) (dic model). when ligation of the cecum is omitted, no disease symptoms develop. in contrast, the animals are primed which becomes apparent as vibriocidal protection upon challenge with v. cholerae in the dic model. this protection coincides with high anti-o antigen iga levels in the bile. the o antigen was shown to be the protective antigen and it must be presen ...	1988	2464884
normal and regenerating optic fibers in goldfish tectum: hrp-em evidence for rapid synaptogenesis and optic fiber-fiber affinity.	the distribution of normal and regenerating retinal fibers and synapses was studied on tectum in goldfish by light (lm) and electron microscopy (em). since labeling of the early regenerating fibers was previously reported to be difficult, a new 'cold-fill' hrp labeling protocol was developed, which labeled regenerating optic fibers and terminals on tectum as early as 14 days after nerve crush when they first arrive on tectum. in order to characterize the laminar distribution of optic afferents i ...	2007	2464622
normal and regenerating optic fibers in goldfish tectum: hrp-em evidence for rapid synaptogenesis and optic fiber-fiber affinity.	the distribution of normal and regenerating retinal fibers and synapses was studied on tectum in goldfish by light (lm) and electron microscopy (em). since labeling of the early regenerating fibers was previously reported to be difficult, a new 'cold-fill' hrp labeling protocol was developed, which labeled regenerating optic fibers and terminals on tectum as early as 14 days after nerve crush when they first arrive on tectum. in order to characterize the laminar distribution of optic afferents i ...	2007	2464622
the development of a bivalent vaccine against diarrhoeal disease.	the live oral typhoid vaccine salmonella typhi ty2la has been successfully used as an effective public health tool for the control of typhoid fever. this paper reviews the progress of one vaccine development programme, which uses this organism as a carrier of the o-antigens of vibrio cholerae. it is already known that antibodies directed against the o-antigens have been previously demonstrated in animals to be protective against subsequent challenge with virulent organisms. this paper reports th ...	1988	2464195
immunoglobulins in bile and serum of the rabbit associated with protection after vibrio cholerae infection and vaccination.	previous studies have shown that cholera, as well as protective immunity against infection with vibrio cholerae, can be induced in the rabbit. this protection is long-lasting (up to 30 months) and is characterized on challenge by rapid, symptom-free disappearance of v. cholerae from the intestine; we therefore believe this to be vibriocidal protection. in this study, we analysed the humoral and secretory immune response against various subcellular v. cholerae components in vibriocidally protecte ...	1987	2462153
[environmental phages active in relation to vibrios of the non-01 group].		1988	2462125
[the population composition of cultures of vibrio cholerae strain 569 b pakistan in relation to the manufacture of a cholera vaccine (choleragen-anatoxin+o-antigen)].	the study of different subcultures of v. cholerae strain 569 b pakistan, used as the source of choleragen in the production of cholera vaccine, has been made. the composition of the population formed by this strain has been found to be heterogeneous with respect to toxin formation. the control of changes occurring in the production culture by analyzing its population composition has been proposed.	1988	2461623
[a minimal synthetic medium for determining cystine desulfohydrolase in vibrio cholerae].		1988	2461482
[auxotrophic mutants of cholera and nonagglutinating vibrios and a method of their determination].		1988	2459483