Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| leptomeningeal plasmacytosis. case report and considerations on treatment. | we describe a 68-year-old patient with a plasma cell leukaemia in haematological remission presenting with massive intracranial leptomeningeal plasmocytic infiltration (lpi) and hydrocephalus. he was treated with skull irradiation and a combination of intraventricular and lumbar intrathecal therapy with methotrexate. neurologic improvement and clearance of plasma cells from the cerebrospinal fluid was reached after 2 weeks of treatment but prolonged follow-up was interrupted by a lethal gastro-i ... | 1963 | 2555099 |
| cerebral toxoplasmosis in the acquired immune deficiency syndrome. | we examined the clinical and pathological features of 26 patients who presented with a diagnosis of cns toxoplasmosis. patient data was analyzed with respect to demographics, clinical presentation, treatment course and pathology. patients presented with a wide variety of signs and symptoms. all patients had positive serum antitoxoplasma igg; ring enhancing lesion(s) were present on all but one brain ct scans. a series of guidelines in the management of cns toxoplasmosis in aids patients are pres ... | 1963 | 2555089 |
| angiotensin ii modulates cardiac na+ channels in neonatal rat. | since chronic congestive heart failure syndromes are associated with both elevated circulating levels of angiotensin ii and potentially lethal ventricular tachyarrhythmias, we investigated the effect of angiotensin ii on voltage-dependent cardiac na+ currents. single-channel na+ currents in neonatal rat ventricular myocytes were studied using the patch clamp method in the cell-attached mode. angiotensin ii applied outside the patch increased the frequency of opening and rates of activation and i ... | 1989 | 2555083 |
| developmental changes in guanine nucleotide regulatory proteins in the rat myocardial alpha 1-adrenergic receptor complex. | during development, the cardiac alpha 1-adrenergic chronotropic response changes from positive in the neonate to negative in the adult. the negative chronotropic effect of alpha 1-adrenergic stimulation in the adult depends on maturation of sympathetic innervation and the presence of a pertussis toxin (pt)-sensitive guanine nucleotide-binding (g) protein. to examine the possibility of a developmental change in coupling of a pt-sensitive g protein to the alpha 1-adrenergic receptor, radioligand b ... | 1989 | 2555081 |
| [establishment of immunohistochemical technique for polyamine and its application in research of lung cancer]. | antiputrescine, antispermidine and antispermine were used to detect polyamine in normal and cancer tissues of lung by pap method. sections of lung cancer, which were known to have high concentration of polyamine, were used as positive control. results showed highly significant difference in the staining between the experimental and control groups. immunohistochemical assay further demonstrated that polyamine located mainly in the nucleus, nucleolus and cytoplasm of the cancer cells and was evenl ... | 1964 | 2555074 |
| in vitro pharmacological properties of a series of isoprenaline derivatives. | the p-trifluoromethylanilide congener of isoprenaline, tert-butyl n-[(s)[( 4-[(r)-6-[2-(3,4-dihydroxyphenyl-2- hydroxyethyl]amino]heptanamido]phenyl]methyl][(n-methylcarbamoy l) methyl]carbamoyl]methyl]carbamate (1s,4r)-4,7,7-trimethyl-3-oxo-2- oxabicyclo[2.2.1]heptane-1-carboxylate (1:1) (ro 17-2218) was investigated for its effects in various pharmacological tests in vitro and compared to the parent compound. as ro 17-2218 represented a mixture of four diastereomers, the pure isomers were synt ... | 1989 | 2554933 |
| effects of adenosine analogues on basal, prostaglandin e1- and forskolin-stimulated cyclic amp formation in intact neuroblastoma cells. | we have examined the effects of r-phenylisopropyladenosine (r-pia) and other adenosine analogues on basal, prostaglandin e1 (pge1)- and forskolin-stimulated cyclic amp (camp) formation in intact n1e-115 neuroblastoma cells, to determine whether the cells contain a1 adenosine receptors that are negatively coupled with adenylate cyclase. basal levels of camp (68 +/- 7 pmol/mg protein; mean +/- se, n = 15) were not altered by low concentrations of r-pia. the apparent lack of inhibition was not due ... | 1967 | 2554919 |
| alveolar cell carcinoma complicating sickle cell anemia: a chance occurrence? | alveolar cell carcinoma, a neoplasm associated with pre-existing inflammatory lung disease, has not been reported previously in patients with sickle cell disease. we present such a patient and suggest that chronic pulmonary scarring in this setting predisposed to development of his malignancy. as survival increases in individuals with sickle cell disease, alveolar cell carcinoma must be considered in these differential diagnosis of otherwise unexplained chronic pulmonary infiltrates. | 1963 | 2554726 |
| dietary intake and colon cancer: sex- and anatomic site-specific associations. | a case-control study was conducted in utah between july 1979 and june 1983 in which 231 cases of colon cancer identified through the utah cancer registry and 391 controls identified through random digit dialing were interviewed. odds ratios (or) were calculated comparing the highest exposure categories with the lowest exposure categories. the highest quintile of body mass index (weight (kg)/height (m)2 for males; weight (kg)/height (m)1.5 for females) was associated with increased risk in both m ... | 1963 | 2554725 |
| intrahepatic cholesterol stones associated with peripheral cholangiocellular carcinoma: an autopsy case. | a rare autopsy case of intrahepatic cholesterol stones associated with peripheral intrahepatic cholangiocellular carcinoma is presented. the patient, a 60-yr-old man, was diagnosed as having peritoneal dissemination of adenocarcinoma, and died of respiratory failure 4 months later. at autopsy, intrahepatic cholangiocellular carcinoma was found at the left lateral segment of the liver. the cancer was extensively disseminated to the peritoneal cavity. in addition, the liver harbored a cholesterol ... | 1989 | 2554723 |
| antiviral drugs in pediatrics. | pediatricians are made familiar with antiviral drugs and are provided with specific recommendations for treatment of viral diseases. the antiviral drugs in clinical use today are discussed in terms of their doses, routes of administration, mechanisms of action, established and potential efficacies, and toxicities. these drugs include acyclovir, amantadine, trisodium phosphonoformate, ganciclovir, ribavirin, rimantadine, vidarabine, and zidovudine. biologic response modifiers, such as interferons ... | 1989 | 2554721 |
| comparison of four latex agglutination (la) and three enzyme-linked immunosorbent assays (elisa) for the detection of rotavirus in fecal specimens. | eighty-two stool specimens obtained from children with gastrointestinal disease were tested for the presence of antigen to rotavirus by latex agglutination (la) (virogen (vr), meritec (mer), wellcome (wel), slidex rotatest (srt), and enzyme-linked immunosorbent assays (rotaclone [trc], rotazyme ii [rtz], pathfinder [pth]). confirmatory testing was performed by isolation of rotavirus from stool specimens with the use of a shell vial centrifugation, antigen-detection tissue culture amplification m ... | 1989 | 2554719 |
| prediction of 'high-risk' cervical papillomavirus infection by biopsy morphology. | certain human papillomavirus (hpv) types (such as type 16) have been linked to high-grade precancers and invasive carcinomas of the cervix. however, the accuracy with which morphologic characteristics will predict the presence and type of hpv infection is controversial. three pathologists independently classified 102 consecutive cervical biopsies with the use of specific criteria and correlated their findings with the presence of hpv 11, 16, and 18 rna sequences by in situ hybridization. based o ... | 1989 | 2554717 |
| hydrophobic calcium channel ligands: methodical problems and their solution. | niguldipine is a 1,4-dihydropyridine derivative that combines l-type ca2+ channel-blocking effects and alpha 1-adrenolytic activity within a single molecule, exemplifying a novel approach in the treatment of hypertension. as niguldipine is a very hydrophobic compound, it (1) readily adsorbs to surfaces of the plastic-ware often used in radioligand binding assays and (2) partitions into the hydrophobic membrane compartments. both phenomena decrease the actual free drug concentration in radioligan ... | 1989 | 2554713 |
| age-related changes in k+ transport and synaptic transmission during and after anoxia in rat hippocampal slices. | 1989 | 2554681 | |
| antigen presentation by b cells and macrophages of cytochrome c and its antigenic fragment when conjugated to the surface of liposomes. | an in vitro antigen presentation system was used to study how antigens coupled to the surface of phospholipid vesicles (liposomes) are presented to antigen specific t cells. liposome-bound pigeon cytochrome c (pcc) was 30-40-fold more potent than free pcc when peritoneal macrophages were the presenting cell. b cells presented surface-bound pcc, albeit less efficiently than unmodified pcc. surface-bound peptide epitope was presented by both cell types, but not as efficiently as unmodified peptide ... | 1969 | 2554605 |
| [assessment of an analysis of the connections of nutrition with risk factors for ischemic heart disease in a one-time epidemiologic study]. | nutrition of 908 males aged 20-54 years, living in tallinn, was studied in simultaneous population investigation by the method of one-day questioning. it was found that subjects with excessive body mass (ebm) and coronary heart disease (chd), who did not keep to a diet, reduced their ration calorific value, consumption of saturated fatty acids, sugar, and increased consumption of polyunsaturated fatty acids and food fibres. no relations were detected between nutrition and chd risk factors in the ... | 1968 | 2554595 |
| expression of avian reticuloendotheliosis virus envelope confers host resistance. | we constructed two reticuloendotheliosis virus (rev) envelope gene expression plasmids, one containing the rev-a envelope gene, the other the spleen necrosis virus (snv) envelope gene. cell lines were generated by transfecting each of the rev envelope plasmids into d17 cells, a canine cell line. the levels of rev envelope glycoprotein in the cell lines were assayed by immunoprecipitating the envelope glycoproteins from lysates of cells that were labeled with [35s]methionine. virological challeng ... | 1989 | 2554569 |
| p53: a frequent target for genetic abnormalities in lung cancer. | allele loss is a hallmark of chromosome regions harboring recessive oncogenes. lung cancer frequently demonstrates loss of heterozygosity on 17p. recent evidence suggests that the p53 gene located on 17p13 has many features of such an antioncogene. the p53 gene was frequently mutated or inactivated in all types of human lung cancer. the genetic abnormalities of p53 include gross changes such as homozygous deletions and abnormally sized messenger rnas along with a variety of point or small mutati ... | 1989 | 2554494 |
| [respiratory viral infections in young children 1985-1988]. | in the period from may 1985 to june 1988 the authors (using the immunofluorescence method) examined 848 children aged 0-2 years hospitalized due to infections of respiratory tracts in the ii clinic of pediatrics of the pediatrics department of the ii medical faculty in the medical academy in warsaw. the most frequent causes of infections in respiratory tracts were virus rs (21.1% of patients) and virus of parainfluenza type 3 (6.5% of patients). an increase in viral respiratory infections took p ... | 1989 | 2554369 |
| effects of repeated trazodone administrations on serotonergic neurotransmission: biochemical studies. | 1. repeated administrations of trazodone as well as imipramine or mianserin (10 mg/kg i.p. twice daily for 3 weeks) attenuated the norepinephrine (ne) stimulation of adenylate cyclase studied in brain minces. therefore trazodone shares with "tricyclic" (imipramine) and "atypic" (mianserin) antidepressants the capability to modulate the beta-adrenergic function. 2. daily treatments with imipramine or trazodone enhanced the vmax of neural uptake of serotonin (5ht) in minces prepared from rat front ... | 1989 | 2554363 |
| molecular cloning and sequence of cdna encoding polyoma medium tumor antigen-associated 61-kda protein. | polyoma virus medium tumor antigen forms specific complexes with several cellular proteins; among these is a protein of approximately 61 kda. with antibodies directed against medium tumor antigen, the 61-kda protein was purified from human 293 cells that were infected with a hybrid adenovirus and overexpressed medium tumor antigen. the purified 61-kda protein was partially digested with protease v8, and one of the protease v8 fragments was isolated and partially sequenced. the amino acid sequenc ... | 1989 | 2554323 |
| transcription terminates near the poly(a) site in the cyc1 gene of the yeast saccharomyces cerevisiae. | a 38-base-pair region required for normal cyc1 mrna 3' end formation in saccharomyces cerevisiae was shown to be necessary for the termination of transcription in vivo by examining the stability of cen3 plasmids. cen3 plasmids were stably maintained during vegetative growth, unless a gal1 transcript impinged on the cen3 region. transcription from the gal1 promoter was terminated, and plasmid stability was restored by the insertion of a fragment containing the 38-base-pair region of cyc1. in cont ... | 1989 | 2554310 |
| cell proteins bind to multiple sites within the 5' untranslated region of poliovirus rna. | the 5' noncoding region of poliovirus rna contains sequences necessary for translation and replication. these functions are probably carried out by recognition of poliovirus rna by cellular and/or viral proteins. using a mobility-shift electrophoresis assay and 1,10-phenanthroline/cu+ footprinting, we demonstrate specific binding of cytoplasmic factors with a sequence from nucleotides 510-629 within the 5' untranslated region (utr). complex formation was also observed with a second sequence (nuc ... | 1968 | 2554308 |
| evidence for site-specific absorption of a novel ace inhibitor. | moexipril [2-[(1-ethoxycarbonyl)-3-phenylpropyl]amino-1-oxopropyl]-6, 7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid (s,s,s)], an ester prodrug of an ace inhibitor, was formulated in controlled-release preparations with a range of in vitro release rates, to provide a prolonged input of drug in vivo. however, pharmacokinetic studies with the controlled-release dosage forms in humans produced plasma profiles with the same characteristics and time to peak as an immediate-release capsu ... | 1967 | 2554270 |
| [clinical and biological aspects of intensive chemotherapy with autologous bone marrow grafts in small-cell bronchial cancer]. | small cell lung cancer (sclc) is a frequent and aggressive tumor characterized by its major sensitivity to chemotherapy since 80% of the patients respond dramatically to the treatment. nevertheless, the majority of them eventually die from their cancer. we have addressed the effect of late intensification with autologous bone marrow transplantation on sclc through a randomized clinical trial. this study included 101 patients, 45 of whom could be randomized to either conventional post-induction t ... | 1989 | 2553180 |
| use-dependent block of sodium channels in frog myelinated nerve by tetrodotoxin and saxitoxin at negative holding potentials. | na+ currents were measured in myelinated frog nerve fibres in the presence of nanomolar concentrations of tetrodotoxin (ttx) or saxitoxin (stx) in the extracellular solution. the na+ currents declined during a train of depolarizing pulses if the fibre was held at hyperpolarizing potentials between the pulses. at a pulse frequency of 0.8 hz, the peak na+ currents were reduced to 70 or 60% of the initial value in 9.3 nm ttx and 3.5 nm stx solutions, respectively. a decline of na+ currents was also ... | 2008 | 2553115 |
| effects of chronic antidepressant and benzodiazepine treatment on corticotropin-releasing-factor receptors in rat brain and pituitary. | we examined the effects of chronic treatment with antidepressants (imipramine or desipramine) or benzodiazepines (diazepam, alprazolam, or adinazolam) on modulation of corticotropin-releasing-factor (crf) receptors in discrete areas of rat brain and in anterior pituitary. as previously reported, we found that chronic antidepressant treatment downregulated 5-ht2 serotonin and beta-adrenergic receptors in cerebral cortex. although there was a trend toward increased crf binding in brain stem, stria ... | 1989 | 2553044 |
| binding modes of mammalian hepatic gal/galnac receptors. | mammalian gal/galnac receptors show dramatic preference for three-branched oligosaccharide structures over two- or one-branched counterparts. the spatial arrangement of the gal residues is extremely important for optimal binding. the three terminal gal residues in the best triantennary ligand are about 15-30 a apart, and therefore the sugar-combining sites on the receptor may also have the same geometry. the results obtained with synthetic ligands containing gal or galnac are in agreement with t ... | 2008 | 2551610 |
| mapping of chromosomal loci associated with lipopolysaccharide synthesis and serotype specificity in vibrio cholerae 01 by transposon mutagenesis using tn5 and tn2680. | vibrio cholerae strains of the 01 serotype have been classified into three subclasses, ogawa, inaba and hikojima, which are associated with the o-antigen of the lipopolysaccharide (lps). the dna encoding the biosynthesis of the o-antigen, the rfb locus, has been cloned and analysed (manning et al. 1986; ward et al. 1987). transposon mutagenesis of the inaba and ogawa strains of v. cholerae, using tn5 or tn2680 allowed the isolation of a series of independent mutants in each of these serotypes. s ... | 1989 | 2550772 |
| comparison of human, simian, and bovine rotaviruses for requirement of sialic acid in hemagglutination and cell adsorption. | human rotaviruses (wa, kun, mo) showed hemagglutination (ha) only with fixed 1-day-old chicken erythrocytes, and their ha activities were completely destroyed by trypsin activation of virions. simian sa-11 and bovine ncdv had ha activities not only against fixed erythrocytes but also against fresh erythrocytes from various species. their ha activities against fixed erythrocytes were also inhibited by trypsin activation, but those against fresh erythrocytes were not. neuraminidase treatment of fi ... | 1989 | 2549710 |
| cloning and characterization of mutl and muts genes of vibrio cholerae: nucleotide sequence of the mutl gene. | the mutl and muts genes of vibrio cholerae have been identified using interspecific complementation of escherichia coli mutl and muts mutants with plasmids containing the gene bank of v. cholerae. the recombinant plasmid pjt470, containing a 4.7 kb fragment of v. cholerae dna codes for a protein of molecular weight 92,000. the product of this gene reduces the spontaneous mutation frequency of the e. coli muts mutant. the plasmid, designated pjt250, containing a 2.5 kb dna fragment of v. cholerae ... | 1989 | 2549510 |
| interleukin-1 induction by lipopolysaccharides: structural requirements of the 3-deoxy-d-manno-2-octulosonic acid (kdo). | we previously showed the importance of the 3-deoxy-d-manno-2-octulosonic acid (kdo) residue in endotoxins (lipopolysaccharides, lps) for the induction of the synthesis and release of interleukin-1 (il-1) by human monocytes. we further investigated the effect of some structural variations within the kdo molecule on il-1 production induced by lps. deamination of bordetella pertussis lps, followed by mild anhydrous acidic methanolysis released a hexasaccharide (fragment b'), which had a terminal me ... | 1989 | 2549403 |
| the expanding role of cisplatin in the treatment of non-small-cell lung cancer. | cisplatin has been employed in the treatment of non-small-cell lung cancer (nsclc) since the late 1970s. current evidence suggests that it may be the most effective single drug for the treatment of nsclc patients. the overall response rate as a single agent is 21%, though responses are higher in previously untreated patients given high doses. cisplatin combinations are the most effective therapy for advanced nsclc. two-drug combinations with a vinca alkaloid or etoposide consistently provide the ... | 2006 | 2548280 |
| [a germ cell tumor of an ectopic testis in polyorchidism]. | 2008 | 2547104 | |
| hypoxic changes in hippocampal neurons. | 1. reversible effects of brief periods of anoxia (replacing 95% o2-5% co2 with 95% n2-5% co2 for 2-4 min) were studied in ca1 neurons in hippocampal slices (from sprague-dawley rats), kept in an interface-type chamber at 33.5 degree. 2. the predominant voltage change during anoxia (n2) was a hyperpolarization, accompanied by a marked fall in resistance and excitability; synaptic potentials were also depressed, especially inhibitory postsynaptic potentials (ipsps). 3. in voltage-current (v-i) plo ... | 1989 | 2547034 |
| a randomized trial of anticoagulation with warfarin and of alternating chemotherapy in extensive small-cell lung cancer by the cancer and leukemia group b. | the cancer and leukemia group b (calgb) conducted a prospective randomized trial to evaluate the role of warfarin and alternating chemotherapy in extensive small-cell lung cancer (sccl). after stratification for sex and performance status, patients were randomly assigned to receive chemotherapy with methotrexate, doxorubicin (adriamycin; adria laboratories, columbus, oh), cyclophosphamide, and lomustine (ccnu) (macc), or macc plus warfarin (macc + w), or mitomycin, etoposide, cisplatin, and hexa ... | 2008 | 2547030 |
| does dietary fibre stimulate intestinal epithelial cell proliferation in germ free rats? | the aim of the present experiment was to investigate the role of hind gut fermentation in the proliferative response of the intestinal epithelium to dietary fibre. we have previously shown that refeeding starved rats with an elemental diet supplemented with fermentable dietary fibre (but not inert bulk) is capable of stimulating intestinal epithelial cell proliferation throughout the gastrointestinal tract. three groups of 10 germ free (gf) rats and three groups of 10 conventional (cv) rats, wer ... | 1989 | 2546871 |
| what do we measure by a luminol-dependent chemiluminescence of phagocytes? | the review presents a survey of published findings concerning the mechanism of luminol-dependent chemiluminescence in biological systems. the potential of various oxygen species (superoxide anion, hydrogen peroxide, hydroxyl radical) to react with luminol is discussed. the ability of commonly used enzymes (superoxide dismutase, catalase), inhibitors, and oxygen radical scavengers to discriminate between individual oxygen species is assessed together with the potential of a variety of substances ... | 2008 | 2546866 |
| the effects of some possible inhibitors of ectonucleotidases on the breakdown and pharmacological effects of atp in the guinea-pig urinary bladder. | 1. the effects of some possible inhibitors of ectonucleotidases on the breakdown of extracellular atp by strips of guinea-pig urinary bladder were investigated. 2. suramin and ethacrynic acid (10 mm) both inhibited atp breakdown significantly, and difluorodinitrobenzene (10 mm) inhibited it slightly whereas n-ethylmaleimide, adenosine 5'-(gamma-thiotriphosphate) (atp-gamma-s) and reactive blue-2 (10 mm) were without effect. 3. the inhibitory effects of suramin on atp breakdown were non-competiti ... | 1989 | 2546853 |
| hypoglycaemia induced by antibodies to insulin receptor following a bone marrow transplantation in an immunodeficient child. | severe hypoglycaemia developed seven months after a bone marrow transplantation in a child with severe combined immunodeficiency. his serum exerted potent insulin-like activity: (a) it stimulated insulin receptor autophosphorylation and kinase activity in cell-free systems, this effect being additive to insulin; (b) it increased glucose transport in isolated soleus muscle. these insulin-like effects were due to immunoglobulins against the insulin receptor. indeed, the patient serum immunoprecipi ... | 1989 | 2546845 |
| characterization of hormone-sensitive adenylate cyclase in rabbit gallbladder mucosa. | 1. we have developed a plasma membrane preparation from the mucosal epithelium of rabbit gallbladder and have characterized the hormonal sensitivity of adenylate cyclase in this preparation. 2. basal activity is low and is stimulated by gtp and gppnhp. hormonal stimulation is largely dependent on exogenous guanine nucleotide. 3. several prostaglandins (e1 approximately e2 greater than a1 greater than b1), vasoactive intestinal peptide and the beta-adrenergic agonist, isoproterenol, stimulate muc ... | 1989 | 2546833 |
| fine needle aspiration of the lung at the medical center of delaware: a retrospective review of 243 patients. | 2008 | 2546832 | |
| electrophysiological characteristics of neurons in neocortical explant cultures. | we examined the electrophysiological and morphological properties of neocortical neurons maintained in explant cultures prepared from the parietal cortex of newborn sprague-dawley rats. after 3-6 weeks in vitro, cultures showed regional differences in cellular density reminiscent of cortical layering, and an abundance of axonal processes. pyramidal-shaped neurons with spinous dendrites were the dominant elements revealed by lucifer yellow injections. intracellular recordings revealed that many e ... | 1989 | 2546787 |
| the relationship of benzodiazepine binding sites to the norepinephrine projection in the hypothalamus of the adult rat. | the relationship of benzodiazepine binding sites to noradrenergic terminals in different brain regions of adult rats was evaluated. the in vitro addition of 10(-5) m diazepam (dz) significantly reduced the depolarization-induced release of [3h]norepinephrine (ne) in the hypothalamus, while no effect was observed in the cerebellum, suggesting that dz may act directly on ne terminals in the hypothalamus. the action of dz in the hypothalamus was blocked either by co-incubation with the benzodiazepi ... | 1989 | 2546655 |
| stress-induced behavioral depression in the rat is associated with a decrease in gaba receptor-mediated chloride ion flux and brain benzodiazepine receptor occupancy. | rats exposed to inescapable tailshock exhibit deficits in learning a simple shuttlebox escape task 24 h later. this syndrome has been termed 'behavioral depression' or 'learned helplessness', and is a model of stress-induced depression. in the present study a significant (25%) decrease in gaba receptor-mediated chloride ion flux as measured by muscimol-stimulated 36cl- uptake in synaptoneurosomes was found in the cerebral cortices of rats that failed the shuttlebox task as compared to naive cont ... | 1989 | 2546650 |
| differential effects of the destruction of leydig cells by administration of ethane dimethane sulphonate to postnatal rats. | ethane dimethane sulphonate (eds) is a cytotoxic drug that selectively destroys leydig cells in adult testes. this study has examined the effect of a single injection of eds on the leydig cell populations present in the testes of rats aged 5, 10, or 20 days. microscopic examination of the tissue demonstrated that the fetal leydig cell population was destroyed at all ages, but that subsequent development of the adult population of leydig cells was not affected. whilst the destruction of the fetal ... | 1989 | 2546615 |
| [immunoenzyme analysis and latex agglutination reaction: use in diagnosing rotavirus gastroenteritis]. | 1989 | 2543138 | |
| a pertussis toxin-sensitive g protein in hippocampal long-term potentiation. | high-frequency (tetanic) stimulation of presynaptic nerve tracts in the hippocampal region of the brain can lead to long-term synaptic potentiation (ltp). pertussis toxin prevented the development of tetanus-induced ltp in the stratum radiatum-ca1 synaptic system of rat hippocampal slices, indicating that a guanosine triphosphate-binding protein (g protein) may be required for the initiation of ltp. this g protein may be located at a site distinct from the postsynaptic neuron (that is, in presyn ... | 1989 | 2543072 |
| dual function of a nuclear factor i binding site in mmtv transcription regulation. | using linker-scanning mutagenesis we had previously identified four elements within the mmtv ltr which are necessary for transcriptional stimulation by glucocorticoid hormones. two of them overlapped with regions to which the glucocorticoid receptor binds in vitro. the third element contained a nf-i binding site, and the fourth the tata box. here we show that mutations that abolish in vitro binding of nf-i had a negative effect also on the basal activity of the mmtv promoter of ltr-containing pl ... | 1989 | 2542892 |
| multiple cooperative interactions constrain bpv-1 e2 dependent activation of transcription. | transcription directed by the bpv-1 long control region (lcr) is conditional upon activation by the virally encoded e2 protein. within the 1.0 kb lcr there are five separate regions, a to e, that contain e2 responsive enhancers. the smallest functional region, a, is only 38 bp and contains two copies of the consensus sequence acc(n)6ggt that is known to function as an e2 binding site in vitro. we show that a pair of these constitutes a minimal functional e2 responsive enhancer element but that t ... | 1989 | 2542891 |
| a molecular epidemiological study of vibrio cholerae in australia. | two hundred and eight strains of vibrio cholerae serovar o1 and 454 non-o1 strains, which were isolated within or imported into australia, were tested for the production of cholera enterotoxin and for hybridization with gene probes for cholera enterotoxin. genetic relationships among representative isolates were studied by a comparison of the restriction-endonuclease digest patterns of the chromosomal dna and by southern-blot analysis of toxigenic strains with the cholera-toxin gene probe. v. ch ... | 1989 | 2542736 |
| relationship between calcium loading and impaired energy metabolism during na+, k+ pump inhibition and metabolic inhibition in cultured neonatal rat cardiac myocytes. | this study tested the hypothesis that the initiating mechanism is a major determinant of the response to calcium (ca) accumulation in myocardium. cultured neonatal rat ventriculocytes were exposed to na+, k+ pump inhibition with 1 mm ouabain and metabolic inhibition with 20 mm 2-deoxy-d-glucose and 1 mm cyanide (dog-cn) for up to 2 h. microspectrofluorometry of myocytes loaded with fura-2 showed that ouabain resulted in a relatively rapid increase in [ca2+]i up to 2-3 microm (two to threefold ab ... | 1989 | 2542375 |
| gas chromatographic determination of (phosphorylated) 2-keto-3-deoxyoctonic acid, heptoses and glucosamine in bacterial lipopolysaccharides after treatment with hydrofluoric acid, methanolysis and trifluoroacetylation. | quantification of phosphorylated sugar constituents of lipopolysaccharides has been performed by the following sequence: dephosphorylation by treatment with hydrofluoric acid, cleavage to monomeric constituents by methanolysis and analysis of the released sugars by capillary gas chromatography. lipopolysaccharides of salmonella minnesota rd1p+, bordetella pertussis nih 114 and vibrio cholerae, nag and 95r strains, were used as model substances. comparison of the chromatographic data obtained fro ... | 1989 | 2541150 |
| broad-host-range vectors for delivery of tnphoa: use in genetic analysis of secreted virulence determinants of vibrio cholerae. | gene fusions between the cholera toxin structural genes and phoa, which encodes bacterial alkaline phosphatase, were identified after tnphoa mutagenesis of the cloned genes in escherichia coli and were then mobilized into vibrio cholerae. the activities of the hybrid proteins were detectable in v. cholerae and suggested that, like cholera toxin, they were secreted beyond the cytoplasm. to extend the utility of tnphoa to identify additional genetic export signals in v. cholerae and other gram-neg ... | 1989 | 2539354 |
| coordinate regulation and sensory transduction in the control of bacterial virulence. | genes and operons that encode bacterial virulence factors are often subject to coordinate regulation. these regulatory systems are capable of responding to various environmental signals that may be encountered during the infectious cycle. for some pathogens, proteins that mediate sensory transduction and virulence control are similar to components of other bacterial information processing systems. understanding the molecular mechanisms governing global regulation of pathogenicity is essential fo ... | 1989 | 2537530 |
| topographical mapping of calmodulin-target enzyme interaction domains. | calmodulin derivatives, specifically biotinylated in domains i and iii, were synthesized to address the structures of calmodulin necessary for binding to its target enzymes in active conformations. by binding avidin to these biotinylated calmodulins, the role of specific sequences of the calmodulin molecule in target enzyme interactions could then be evaluated. the role of domain i in these interactions was assessed by biotinylation of cys-27 of wheat germ calmodulin with n-ethylmaleimidobiotin. ... | 1989 | 2536706 |
| crystallization and preliminary x-ray analysis of porin from rhodobacter capsulatus. | porin monomers of the phototrophic bacterium rhodobacter capsulatus were purified. crystals were obtained from a solution of porin solubilized with the detergent octyltetraoxyethylene within 5 days using the vapor phase equilibration technique. the crystals were rhombohedral with an edge length of 0.4 mm. the space group was trigonal r3 with unit cell constants of a = b = 95 a, c = 147 a. reflexions were observed to 3.2 a. | 1989 | 2536626 |
| bile acid profile in a dog with cholangiocarcinoma. | 1989 | 2536512 | |
| aggregation by fragilis and non-fragilis bacteroides strains in vitro. | bacteroides fragilis is associated with the formation of intra-abdominal abscesses, whereas other bacteroides species are rarely involved. since bacterial clumping may contribute to the survival of bacteria in the face of host defence mechanisms, the hypothesis has been put forward that differences in aggregation between fragilis and non-fragilis strains of bacteroides may account for their differences in survival in vivo. all seven b. fragilis strains tested formed aggregates within 4 h, but st ... | 1989 | 2536442 |
| newly developed transarterial chemoembolization material: cddp-lipiodol suspension. | cis-diamminedichloroplatinum (cddp)-lipiodol suspension (cls) was developed as a transarterial chemoembolization (tace) material. the cls was injected into the proper hepatic artery or distal branches of patients with hepatocellular carcinoma. strong anticancer effects were observed. plasma concentrations of total and filtrable cddp following tace were much lower than those following intravenous administration of cddp solution. no major adverse complications were noted, presumably due to low cdd ... | 1982 | 2535992 |
| correlation between selective inhibition of the cyclic nucleotide phosphodiesterases and the contractile activity in human pregnant myometrium near term. | the present study was carried out to determine the ability of various pharmacological agents to selectively inhibit each cytosolic form of phosphodiesterase isolated from the longitudinal layer of human myometria near term. among the drugs tested, zaprinast specifically inhibits the first form of pde which hydrolyses both substrates (camp and cgmp) and is stimulated by the ca2+-calmodulin complex. a second form of pde specific for camp hydrolysis and ca2+-calmodulin insensitive is only present d ... | 1989 | 2535936 |
| inhibitory effect of dibutyryl cyclic adenosine monophosphate on the induction of alkaline phosphatase in human fetal liver cell line. | when human fetal liver cells (hul-1-317), cultured continuously in a serum-free medium, were incubated with a combination of prednisolone, butyrate and a hypertonic concentration of nacl at 37 degrees c, alkaline phosphatase activity increased. however, the addition of dibutyryl adenosine cyclic monophosphate (bt2camp) to these agents inhibited the increase in alkaline phosphatase activity in a dose-dependent manner: the inhibitory effect of bt2camp was significant at 0.05 mm, but disappeared at ... | 1989 | 2535785 |
| the effectiveness of pro cardiac pacemaker review in reducing implantation in the medicare population. | cardiac pacemaker insertions experienced an explosive growth in utilization following the introduction of this highly beneficial technology. cost considerations prompted critical evaluation of the appropriateness of their use and raised the specter of potential abuse. the connecticut peer review organization (cpro) program of pacemaker review to medicare patients, based on physician input, criteria setting, dissemination of information, and specialist peer review, resulted in a dramatic reductio ... | 2006 | 2535580 |
| ultrastructure of human granulosa cells obtained from follicular fluid aspirates. | samples of the granulosa cells obtained from a punctured human preovulatory follicles stimulated for in vitro fertilization purposes were studied by means of light and electron microscopic methods. early and late preluteinized granulosa cells were characterized by the presence of high amount of pale and homogeneous lipid droplets, moderate accumulation of glycogene and progressive development of smooth endoplasmic reticulum. a new type of granulosa cell containing dense-cored granules with fine ... | 1989 | 2533848 |
| isolation, characterization and immunological determination of basement membrane-associated heparan sulfate proteoglycan. | basement membrane-associated heparan sulfate proteoglycan (hspg) was extracted from isolated porcine glomerular basement membranes and purified by ion-exchange chromatography. the proteogycan was characterized by specific enzymatic digestions, by amino-acid analysis, by sds-polyacrylamide gel electrophoresis and by density gradient centrifugation. polyclonal antibodies were raised against the purified hspg in rabbits. antibodies were characterized by enzyme immunoassays, immunoprecipitation and ... | 1989 | 2533508 |
| coronal and sagittal plane spinal deformities correlating with back pain and pulmonary function in adult idiopathic scoliosis. | one hundred one referred adult patients (ages 20-63; mean, 36 years) with painful idiopathic scoliosis were evaluated. none had prior surgical treatment. severity of pain was graded and localized over radiographic deformities in the coronal and sagittal planes. radiographic changes in primary as well as full and fractional compensatory curves were studied. degrees of scoliosis, percent correction on side bending, vertebral body rotation at curve apex, spinal balance, and lateral olisthesis in th ... | 2008 | 2533406 |
| providing for the needs of handicapped candidates. | 2008 | 2533188 | |
| [ischemic colitis and recurrent venous thrombosis caused by familial protein s deficiency]. | 2008 | 2533119 | |
| cell surface sialic acid affects immunoglobulin binding to macrophages. | we demonstrate that guinea pig peritoneal macrophages pretreated with neuraminidase from vibrio cholerae bind more 125i-igg than non-treated cells. estimation of binding constants (ka and bmax) shows that the elevation of binding is the result of an increase in affinity and not in the number of receptors for igg. the change of affinity is proportional to amounts of sialic acid liberated from the cells by increasing doses of neuraminidase. it is also shown that affinity of interactions of igg wit ... | 1989 | 2532157 |
| [clinical efficacies of schizophyllan (spg) on advanced gastric cancer]. | immunochemotherapy using the antitumor polysaccharide sizofiran (spg), an extract from the culture broth of schizophyllum, was prescribed randomly for unresectable or resectable gastric cancer patients. in case of unresectable cases, the spg group (treated by spg plus tegafur--f method--or 5-fu combined with mmc--mf method--) survived longer (p = 0.009 in the mf method and p = 0.003 in the f method) than did the control group (chemotherapy only). concerning a treatment regimen for resectable cas ... | 2008 | 2531270 |
| muscarinic receptor activation stimulates b-50/gap43 phosphorylation in isolated nerve growth cones. | a characteristic feature of neurite formation is high expression of the phosphoprotein b-50/gap43. previous studies with growth cone membranes have indicated that this neuron-specific protein kinase c substrate may be involved in transmembrane signal transduction at the growth cone. we monitored the degree of phosphorylation of b-50 by quantitative b-50 immunoprecipitation from intact nerve growth cones, isolated from 5-day-old rat brain and prelabeled with 32p-orthophosphate. b-50 phosphorylati ... | 1989 | 2531215 |
| plasmid vectors for constructing translational fusions to the b subunit of cholera toxin. | a family of plasmid cloning vectors has been developed for creating translational fusions to the ctxb gene encoding the b subunit of cholera toxin (ctb) in escherichia coli. these vectors permit insertion of transcriptionally and translationally competent gene sequences upstream from ctxb. to test the utility of the system, a portion of the glucosyltransferase b (gtf) gene (gtfb) from the cariogenic bacterium streptococcus mutans gs-5 (bratthall serotype c), encoding the n-terminal one-third of ... | 1989 | 2531107 |
| [an acute myocardial infarction register in yerevan]. | 2008 | 2528017 | |
| hepatitis b vaccination in ramathibodi health personnel 1986-1987. | hevac-b-pasteur 5 mcg, h-b-vax i 20 mcg, and engerix b 20 mcg, were given to 23, 34, 15 health personnel in ramathibodi hospital. the seroconversion rate for pasteur vaccine was 85 per cent compared to 95 per cent for the other two groups. adverse reactions were few and transient. | 2008 | 2527940 |
| success with the young. | 2008 | 2527706 | |
| first international symposium on cilazapril preclinical and clinical pharmacology. lausanne, switzerland, may 5-7, 1988. proceedings. | 2008 | 2527527 | |
| a simple and inexpensive method for preparing erythrocyte membranes by filtration through a hollow-fiber system. | an efficient, simple method for preparing biologically active erythrocyte membranes is described. the semi-automated procedure involves circulating hemolysate mixture through a hollow-fiber system, thereby filtering off intracellular components leaving a high yield of washed ghosts. the prepared ghosts exhibit cation-stimulated atpase activities comparable to those of ghosts prepared by traditional methods. electron micrographs revealed that the filtration isolation caused less shearing of the m ... | 2008 | 2527009 |
| treatment of chronic occlusive arterial disease with pentoxifylline: changes in clinical and haemodynamic parameters. | a prospective study was carried out in 15 patients with peripheral obstructive arterial disease (fontaine stage ii), with obstructions in the thigh region, to assess the effect of treatment with pentoxifylline on clinical and haemodynamically measurable factors, particularly changes in the post-ischaemic increase in peripheral blood pressure. after a 7-day wash-out period, patients were hospitalized for 1 week and received a daily infusion of 300 mg pentoxifylline plus a 600 mg tablet orally in ... | 2007 | 2526340 |
| [comparative evaluation of regulated and non-regulated periods of work and rest in a high-temperature microclimate in the glass industry]. | during warm periods of the year under unregulated work regimen the thermal state of the workers of glass manufacturing hot shops was regarded as discomfort. under regulated work regimen the indices of workers' thermal state and hemodynamics were stabilized at a lower level. the dynamics of changes of the central nervous neuromuscular systems was also more favourable compared to the unregulated regimen. it was considered advisable to restrict the time of continuous stay at the workplace with heat ... | 2006 | 2526060 |
| structure of an anti-plasmin inhibitor, eckol, isolated from the brown alga ecklonia kurome okamura and inhibitory activities of its derivatives on plasma plasmin inhibitors. | eckol (1), a novel phlorotannin with a dibenzo-1,4-dioxin skeleton, has been isolated from the brown alga ecklonia kurome okamura as a potent and specific anti-plasmin inhibitor. its structure has been elucidated based on the spectral data, in particular, by means of negative nuclear overhauser effect (noe), and finally established as 1-(3,5-dihydroxyphenoxy)-2,4,7,9-tetrahydroxydibenzo-1,4-dio xin by x-ray analysis. some partially methoxylated derivatives of eckol were prepared by methylation w ... | 2008 | 2525966 |
| [non 01 vibrio cholerae septicemia]. | 1989 | 2525717 | |
| renal effects of nonsteroidal anti-inflammatory drugs and thromboxane receptor antagonists in chronic glomerular disease. | 2008 | 2522275 | |
| neuraminidase pretreatment of donor lymphocytes and graft-versus-host disease. | treatment of murine cba splenocytes with vibrio cholerae neuraminidase (vcn) prior to engraftment into cyclophosphamide (cy) immunosuppressed balb/c x cba mice reduced the incidence of acute lethal graft-versus-host disease (gvhd) and delayed mortality in the later phase of the disease. in the same model, pretreatment of donor splenocytes with vcn also reduced splenomegaly. engraftment of f1 mice with cba cells was clearly demonstrated at day 30 after infusion. treatment of spleen cells with vcn ... | 1989 | 2521829 |
| permethrin-impregnated bednets reduce nuisance arthropods in gambian houses. | the prevalence of bedbugs (cimex hemipterus l.), chicken ticks (argas persicus oken) and headlice (pediculus capitis de geer) was surveyed in a rural area of the gambia. at the beginning of the study 37.5% of children's beds were infested with bedbugs and 3.9% with chicken ticks, whilst the prevalence rate of pediculosis in children under 10 years old was 28.8%. both bedbugs and headlice were clustered within compounds. headlice prevalence increased with hair length and they were more common on ... | 1989 | 2519687 |
| the disconnected visual system mutations in drosophila melanogaster drastically disrupt circadian rhythms. | mutations at the disconnected (disco) locus in drosophila melanogaster cause cultures of this insect to eclose in an essentially arrhythmic manner and also nearly eliminate free-running circadian rhythms of locomotor activity. yet disco mutants are not totally light-insensitive: whereas they performed very poorly in tests of certain behavioral responses to visual stimuli, they were able to exhibit "forced" periodic locomotor activity under conditions of light-dark cycling. we discuss these resul ... | 2008 | 2519577 |
| diagnostic and therapeutic procedures with haptens and glycoproteins (antigens and antibodies) coupled covalently by specific sites to insoluble supports. | 2008 | 2519545 | |
| killing of human tumor cells by antibody c3b conjugates and human complement. | to potentiate the lytic action of complement on tumor cells, we have constructed heteroconjugates composed of monoclonal antibody and of the human c3b component of complement. the conjugates were formed efficiently using the heterobifunctional cross-linking reagent spdp. the monoclonal antibody-c3b conjugate promoted the killing of k562 tumor cells by normal human serum. treatment of the tumor cells with the monoclonal antibody and normal human serum resulted in 10-15% lysis. however, following ... | 1989 | 2519543 |
| removal and reimplantation of the parietal cortex of mice during the first nine days of life: consequences for the barrelfield. | vibrissal follicles on one side of the mouse whiskerpad are topologically connected to barrels in the contralateral somatosensory cortex. barre's develop from postnatal day 3 to 6. recently, i have observed that the barrelfields still develop in pieces of parietal cortex that were removed and reimplanted, in the same place and with the original orientation, on the day of birth, or on postnatal days 1 or 3. now, two questions were asked: (i) can the barrelfield form and/or remain in place after i ... | 1989 | 2519518 |
| a mechanism for cross-lineage gene rearrangements in b-cell neoplasm. | the organization of immunoglobulin heavy chain (igh) gene of b precursor cell acute lymphoblastic leukemia (all) was examined in order to elucidate the mechanism causing simultaneous tcr gene rearrangements. study using a 5'd probe lying 20 kb upstream of igh d region genes was useful to distinguish diversity(d)-joining(j) recombination(dj) from variable(v)-dj recombination(vdj). indeed, igh gene structures determined by 5'd study well correlated to those recognized by dj or vdj transcripts. igh ... | 2008 | 2519516 |
| the biology of the scid mutation. | 2008 | 2519515 | |
| cardiac arrest postoperatively in a patient infected with ascaris, the roundworm. | 1989 | 2519513 | |
| [diabetes in the works of a piedmontese physician--g. f. arma da chivasso--of the 16th century]. | 2008 | 2519496 | |
| [the history of science in the thought of federigo enriques and augusto murri]. | 2008 | 2519495 | |
| [acute polyarthritis in a patient with aids. clinical report]. | a homosexual patient known to be hiv positive for 2 years developed fever, herpes labialis, salmonellosis and symmetric polyarthritis. no specific cause was documented for the arthritis which was ascribed to the aids syndrome. partial improvement in symptoms was observed after therapy with cotrimoxazole and indomethacin. musculoskeletal complications of aids are reviewed. | 1989 | 2519452 |
| [determination of plasma levels of phenytoin. evaluation of 2 techniques]. | we compared 2 techniques for determination of plasma levels of phenytoin, liquid-gas chromatography (lgc) and homogeneous enzymatic immunoassay (ei) in samples from 72 patients receiving monotherapy with this drug. determinations were also made in samples of normal plasma with known concentrations of phenytoin. good correlation of both techniques was observed in patient samples containing less than 15 micrograms/ml of the drug but not with higher concentrations. lgc was accurate in determination ... | 2008 | 2519450 |
| [headache of cervical origin]. | we studied 40 patients with cervical spine disease who had headache (group a) with 40 patients with disease and no headache (group b). in addition to clinical and radiologic evaluation, 24 patients in group a and 27 in group b had electromyographic study, which was normal en 18 of the former and 4 of the latter group. abnormal neurologic findings were present only in group b (37% of patients). alterations of the cervical spine were more pronounced in group b. these findings suggest that headache ... | 2008 | 2519433 |
| [assessment of nutritional status in the elderly]. | 2008 | 2519421 | |
| [cardiac tamponade as the first manifestation of cancer]. | we report 3 patients having cardiac tamponade as the initial presentation of cancer. at the onset of symptoms all three patients were incorrectly diagnosed. pulsus paradoxus and cardiomegaly on the chest x-ray were the main clinical features. two dimensional echocardiography was vital revealing massive pericardial effusion with diastolic right atrial and ventricular collapse. all patients had bloody pericardial fluid removed and the histopathology of the metastatic pericardial tissue showed lung ... | 2008 | 2519419 |
| [myocardial perfusion during exercise and rest in coronary patients. evaluation with a thallium 201 analog (isonitrile-technetium 99 m)]. | isonitrile-technetium, a thallium 201 analog, was used for myocardial perfusion imaging in 12 patients with known coronary heart disease. 10-40 mcu of the isotope were injected at maximal effort during a bruce stress test. imaging was performed using 3 projections immediately after exercise and 24 hr later, using a gamma camera attached to a computer. myocardial necrosis was detected with a sensitivity of 89% and specificity of 63%. corresponding figures for stress induced ischemia were 89% and ... | 2008 | 2519396 |