Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [the treatment of aspergillus empyema using open thoraco-myoplasty with the preservation of lung function -- case report]. | invasive aspergillosis is a life threatening complication in immune-compromised patients causing lung tissue destruction. aspergillus empyema requires aggressive multimodality treatment. | 2013 | 24144821 |
| in-silico characterization of β-(1, 3)-endoglucanase (engl1) from aspergillus fumigatus by homology modeling and docking studies. | during the past few years a significant rise in aspergillosis caused by filamentous fungus aspergillus fumigatus has been recorded particularly in immunocompromised patients. at present, there are limited numbers of antifungal agents to combat these infections and the situation has become more complex due to emergence of antifungal resistance and side-effects of antifungal drugs. these situations have increased the demand for novel drug targets. recent studies have revealed that the β-1,3-endogl ... | 2013 | 24143049 |
| laccase production optimization by response surface methodology with aspergillus fumigatus af1 in unique inexpensive medium and decolorization of different dyes with the crude enzyme or fungal pellets. | in the present study, alkaline pretreatment was applied for the enhanced laccase production from rice straw. various process parameters including sodium hydroxide concentration, ph and fermentation temperature were optimized via response surface methodology (rsm) with a box-behnken design (bbd). through regression analysis, it was found that laccase activity was well fitted by a quadratic polynomial equation (r(2)=0.998, adj r(2)=0.995), and the fermentation temperature was the most significant ... | 2013 | 24140539 |
| biosorption characteristics of aspergillus fumigatus for the decolorization of triphenylmethane dye acid violet 49. | this study focuses on the possible use of aspergillus fumigatus to remove acid violet 49 dye (av49) from aqueous solution. in batch biosorption experiments, the highest biosorption efficiency was achieved at ph 3.0, with biosorbent dosage of 3.0 gl(-1) within about 30 min at 40 °c. the langmuir and freundlich models were able to describe the biosorption equilibrium of av49 onto fungal biomass with maximum dye uptake capacity 136.98 mg g(-1). biosorption followed a pseudo-second-order kinetic mod ... | 2014 | 24136473 |
| secretome diversity and quantitative analysis of cellulolytic aspergillus fumigatus z5 in the presence of different carbon sources. | aspergillus fumigatus z5 has a strong ability to decompose lignocellulose biomass, and its extracellular protein secretion has been reported in earlier studies employing traditional techniques. however, a comprehensive analysis of its secretion in the presence of different carbon sources is still lacking. the goal of this work was to identify, quantify and compare the secretome of a. fumigatus z5 in the presence of different carbon sources to understand in more details the mechanisms of lignocel ... | 2013 | 24131596 |
| azole resistance in aspergillus fumigatus: a growing public health concern. | reports from the end of the 2000s forced the medical community to take azole resistance in aspergillus fumigatus into account. not only patients with chronic aspergillus disease, who develop resistance during long-term azole treatment, but also azole-naive patients are at risk, owing to the presence of resistant strains in the environment. the purpose of this review is to overview the latest findings concerning the origin, evolution, and implications of azole resistance in a. fumigatus. | 2013 | 24126719 |
| 2-hydroxyisocaproic acid is fungicidal for candida and aspergillus species. | the amino acid derivative 2-hydroxyisocaproic acid (hica) is a nutritional additive used to increase muscle mass. low levels can be detected in human plasma as a result of leucine metabolism. it has broad antibacterial activity but its efficacy against pathogenic fungi is not known. the aim was to test the efficacy of hica against candida and aspergillus species. efficacy of hica against 19 clinical and reference isolates representing five candida and three aspergillus species with variable azol ... | 2014 | 24125484 |
| aspergillus collagen-like genes (acl): identification, sequence polymorphism, and assessment for pcr-based pathogen detection. | the genus aspergillus is a burden to public health due to its ubiquitous presence in the environment, its production of allergens, and wide demographic susceptibility among cystic fibrosis, asthmatic, and immunosuppressed patients. current methods of detection of aspergillus colonization and infection rely on lengthy morphological characterization or nonstandardized serological assays that are restricted to identifying a fungal etiology. collagen-like genes have been shown to exhibit species-spe ... | 2013 | 24123732 |
| assessment of selection and resistance risk for demethylation inhibitor fungicides in aspergillus fumigatus in agriculture and medicine: a critical review. | an increasing number of publications have claimed that demethylation inhibitor (dmi) fungicides are confronted with resistance development in the fungus aspergillus fumigatus and that the origin of resistant isolates may also be outside the medical area. for resistance risk assessment and sourcing the origin of dmi resistance, the primary exposure events ofa. fumigatus with dmi treatments have been analysed case by case, resulting in the pathogen exposure risk (per). | 2014 | 24123539 |
| laccases involved in 1,8-dihydroxynaphthalene melanin biosynthesis in aspergillus fumigatus are regulated by developmental factors and copper homeostasis. | aspergillus fumigatus produces heavily melanized infectious conidia. the conidial melanin is associated with fungal virulence and resistance to various environmental stresses. this 1,8-dihydroxynaphthalene (dhn) melanin is synthesized by enzymes encoded in a gene cluster in a. fumigatus, including two laccases, abr1 and abr2. although this gene cluster is not conserved in all aspergilli, laccases are critical for melanization in all species examined. here we show that the expression of a. fumiga ... | 2013 | 24123270 |
| contributions of aspergillus fumigatus atp-binding cassette transporter proteins to drug resistance and virulence. | in yeast cells such as those of saccharomyces cerevisiae, expression of atp-binding cassette (abc) transporter proteins has been found to be increased and correlates with a concomitant elevation in azole drug resistance. in this study, we investigated the roles of two aspergillus fumigatus proteins that share high sequence similarity with s. cerevisiae pdr5, an abc transporter protein that is commonly overproduced in azole-resistant isolates in this yeast. the two a. fumigatus genes encoding the ... | 2013 | 24123268 |
| exopolysaccharides and antimicrobial biosurfactants produced by paenibacillus macerans tku029. | paenibacillus macerans tku029 can produce exopolysaccharides (epss; 3.46 g/l) and a biosurfactant (1.78 g/l) in a medium with 2 % (w/v) squid pen powder as the sole carbon/nitrogen source. the biosurfactant can reduce the surface tension of water from 72.30 to 35.34 mn/m at a concentration of 2.76 g/l and reach an emulsification index of 56 % after a 24-h reaction with machine oil. this biosurfactant is stable at 121 °c for 20 min, over a ph range from 3 to 11, and in <5 % salt solutions. it als ... | 2014 | 24122708 |
| distinct mechanisms for spiro-carbon formation reveal biosynthetic pathway crosstalk. | spirotryprostatins, an indole alkaloid class of nonribosomal peptides isolated from aspergillus fumigatus, are known for their antimitotic activity in tumor cells. because spirotryprostatins and many other chemically complex spiro-carbon-bearing natural products exhibit useful biological activities, identifying and understanding the mechanism of spiro-carbon biosynthesis is of great interest. here we report a detailed study of spiro-ring formation in spirotryprostatins from tryprostatins derived ... | 2013 | 24121553 |
| [aspergillus species in hospital environments with pediatric patients in critical condition]. | aspergillus is a group of opportunistic fungi that cause infections, with high morbimortality in immunosuppressed patients. aspergillus fumigatus is the most frequent species in these infections, although the incidence of other species has increased in the last few years. | 2014 | 24120420 |
| gfsa encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of o-glycan in aspergillus nidulans and aspergillus fumigatus. | the cells walls of filamentous fungi in the genus aspergillus have galactofuranose (galf)-containing polysaccharides and glycoconjugates, including o-glycans, n-glycans, fungal-type galactomannan and glycosylinositolphosphoceramide, which are important for cell wall integrity. here, we attempted to identify galactofuranosyltransferases that couple galf monomers onto other wall components in aspergillus nidulans. using reverse-genetic and biochemical approaches, we identified that the an8677 gene ... | 2013 | 24118544 |
| the fumagillin gene cluster, an example of hundreds of genes under vea control in aspergillus fumigatus. | aspergillus fumigatus is the causative agent of invasive aspergillosis, leading to infection-related mortality in immunocompromised patients. we previously showed that the conserved and unique-to-fungi vea gene affects different cell processes such as morphological development, gliotoxin biosynthesis and protease activity, suggesting a global regulatory effect on the genome of this medically relevant fungus. in this study, rna sequencing analysis revealed that vea controls the expression of hund ... | 2013 | 24116213 |
| coinfection of pneumocystis jirovecii and invasive pulmonary aspergillosis in an immunocompromised patient: a diagnostic challenge. | invasive infections caused by aspergillus fumigatus as well as pneumocystis pneumonia are serious and often lethal diseases in severely immunocompromised patients. | 2013 | 24107913 |
| genomic identification of potential targets unique to candida albicans for the discovery of antifungal agents. | despite of modern antifungal therapy, the mortality rates of invasive infection with human fungal pathogen candida albicans are up to 40%. studies suggest that drug resistance in the three most common species of human fungal pathogens viz., c. albicans, aspergillus fumigatus (causing mortality rate up to 90%) and cryptococcus neoformans (causing mortality rate up to 70%) is due to mutations in the target enzymes or high expression of drug transporter genes. drug resistance in human fungal pathog ... | 2014 | 24102473 |
| the complexity of aging: cancer risk among elderly people and infectious risk among those with cancer. | aging is associated with a reduced capability of the immune system to adequately respond to pathogens and to prevent tumor formation. as a consequence of immunosenescence, older people have a higher risk to develop infections as well as cancer. in addition, cancer itself may expose old patients to infections, including opportunistic infections, i.e. pseudomonas aeruginosa, aspergillus fumigatus and cytomegalovirus infection. patients with hematologic malignancies have a higher risk than patients ... | 2013 | 24102281 |
| nkt cells: the smoking gun in fungal-induced asthma? | aspergillus fumigatus is a fungus that is associated with a severe form of asthma, although the precise immunological basis for this disease is unclear. a new study in mice shows that natural killer t (nkt) cells are crucial for progression of a. fumigatus–induced asthma and also identifies a glycolipid antigen from this fungus that seems to drive this nkt cell–mediated inflammatory response (pages 1297–1304). | 2013 | 24100979 |
| isavuconazole (bal4815) pharmacodynamic target determination in an in vivo murine model of invasive pulmonary aspergillosis against wild-type and cyp51 mutant isolates of aspergillus fumigatus. | invasive pulmonary aspergillosis (ipa) continues to rise in concert with increasing numbers of immune suppression techniques to treat other medical conditions and transplantation. despite these advances, morbidity and mortality rates remain unacceptably high. one strategy used to optimize outcomes is antifungal pharmacodynamic (pd) examination. we explored the pharmacodynamics of a new triazole in development, isavuconazole, in a murine neutropenic ipa model. ten a. fumigatus isolates were used, ... | 2013 | 24100500 |
| a functional and structural study of the major metalloprotease secreted by the pathogenic fungus aspergillus fumigatus. | fungalysins are secreted fungal peptidases with the ability to degrade the extracellular matrix proteins elastin and collagen and are thought to act as virulence factors in diseases caused by fungi. fungalysins constitute a unique family among zinc-dependent peptidases that bears low sequence similarity to known bacterial peptidases of the thermolysin family. the crystal structure of the archetype of the fungalysin family, aspergillus fumigatus metalloprotease (afumep), has been obtained for the ... | 2013 | 24100314 |
| antifungal dimeric chalcone derivative kamalachalcone e from mallotus philippinensis. | from the red coloured extract (kamala) prepared through acetone extraction of the fresh whole uncrushed fruits of mallotus philippinensis, one new dimeric chalcone (1) along with three known compounds 1-(5,7-dihydroxy-2,2,6-trimethyl-2h-1-benzopyran-8-yl)-3-phenyl-2-propen-1-one (2), rottlerin (3) and 4'-hydroxyrottlerin (4) were isolated. the structure of compound 1 was elucidated by 1d and 2d nmr analyses that included hsqc, hmbc, cosy and roesy experiments along with the literature comparison ... | 2014 | 24099509 |
| computational approaches for discovery of common immunomodulators in fungal infections: towards broad-spectrum immunotherapeutic interventions. | fungi are the second most abundant type of human pathogens. invasive fungal pathogens are leading causes of life-threatening infections in clinical settings. toxicity to the host and drug-resistance are two major deleterious issues associated with existing antifungal agents. increasing a host's tolerance and/or immunity to fungal pathogens has potential to alleviate these problems. a host's tolerance may be improved by modulating the immune system such that it responds more rapidly and robustly ... | 2013 | 24099000 |
| transcriptional activation of heat shock protein 90 mediated via a proximal promoter region as trigger of caspofungin resistance in aspergillus fumigatus. | invasive aspergillosis is a deadly infection for which new antifungal therapies are needed. heat shock protein 90 (hsp90) is an essential chaperone in aspergillus fumigatus representing an attractive antifungal target. using a thiamine-repressible promoter (pthia), we showed that genetic repression of hsp90 significantly reduced virulence in a murine model of invasive aspergillosis. moreover, substituting the a. fumigatus hsp90 promoter with 2 artificial promoters (potef, pthia) and the candida ... | 2014 | 24096332 |
| fatal-mixed cutaneous zygomycosis-aspergillosis: a case report. | mucorales and aspergillus are molds responsible for infections in immunocompromised patients. in this report, we describe a case of a rare extensively mixed cutaneous infection caused by lichtheimia ramosa, aspergillus fumigatus and aspergillus terreus in a neutropenic patient suffering from an acute leukemia. the fatal outcome of this patient can be attributed to its hematologic malignancy, the extensive nature of the lesions and the resistance of the strains to antifungals. | 2013 | 24085614 |
| effect of physical and chemical properties of oil palm empty fruit bunch, decanter cake and sago pith residue on cellulases production by trichoderma asperellum upm1 and aspergillus fumigatus upm2. | the effect of cultivation condition of two locally isolated ascomycetes strains namely trichoderma asperellum upm1 and aspergillus fumigatus upm2 were compared in submerged and solid state fermentation. physical evaluation on water absorption index, solubility index and chemical properties of lignin, hemicellulose and cellulose content as well as the cellulose structure on crystallinity and amorphous region of treated oil palm empty fruit bunch (opefb) (resulted in partial removal of lignin), sa ... | 2014 | 24085387 |
| azole-resistant aspergillus fumigatus with the environmental tr46/y121f/t289a mutation in india. | 2014 | 24084639 | |
| synthesis and biological evaluation of potent antifungal agents. | the last two decades are characterized by major increases in the incidence of systemic fungal infections caused by the yeast candida albicans, particularly in immunocompromised patients. on the other hand it was observed the increased number of pathogenic microorganisms with multiple resistance to drugs. also there is a big variety of drugs for the treatment of candidiasis, only two drugs are used for the treatment of infections from aspergillus fumigatus. taking into account that the long term ... | 2013 | 24083791 |
| prototype of an intertwined secondary-metabolite supercluster. | the hallmark trait of fungal secondary-metabolite gene clusters is well established, consisting of contiguous enzymatic and often regulatory gene(s) devoted to the production of a metabolite of a specific chemical class. unexpectedly, we have found a deviation from this motif in a subtelomeric region of aspergillus fumigatus. this region, under the control of the master regulator of secondary metabolism, laea, contains, in its entirety, the genetic machinery for three natural products (fumitremo ... | 2013 | 24082142 |
| role of ser-257 in the sliding mechanism of nadp(h) in the reaction catalyzed by the aspergillus fumigatus flavin-dependent ornithine n5-monooxygenase sida. | sida (siderophore a) is a flavin-dependent n-hydroxylating monooxygenase that is essential for virulence in aspergillus fumigatus. sida catalyzes the nadph- and oxygen-dependent formation of n(5)-hydroxyornithine. in this reaction, nadph reduces the flavin, and the resulting nadp(+) is the last product to be released. the presence of nadp(+) is essential for activity, as it is required for stabilization of the c4a-hydroperoxyflavin, which is the hydroxylating species. as part of our efforts to d ... | 2013 | 24072704 |
| unusual causes of fungal rhinosinusitis: a study from a tertiary care centre in south india. | the frequency of mycotic infections of the nose and paranasal sinuses has been increasing over the past three decades. apart from the common causes of fungal rhinosinusitis such as aspergillus species and penicillium species, there have been reports of rare and unusual fungi isolated from india and other countries. | 2015 | 24064646 |
| the impact of aspergillus fumigatus viability and sensitization to its allergens on the murine allergic asthma phenotype. | aspergillus fumigatus is a ubiquitously present respiratory pathogen. the outcome of a pulmonary disease may vary significantly with fungal viability and host immune status. our objective in this study was (1) to assess the ability of inhaled irradiation-killed or live a. fumigatus spores to induce allergic pulmonary disease and (2) to assess the extent to which inhaled dead or live a. fumigatus spores influence pulmonary symptoms in a previously established allergic state. our newly developed f ... | 2013 | 24063011 |
| synthesis and evaluation of novel bis[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles as potent antimicrobial agents. | a series of novel bis[4-methoxy-3-(6-aryl[1,2,4]triazolo[3,4-b][1,3,4]thiadiazol)phenyl]methanes 5a-l has been synthesized and characterized via ir, 1h nmr, 13c nmr, ms and elemental analyses. all the newly synthesized compounds were screened for their antibacterial activity against bacillus subtilis, bacillus sphaericu, staphylococcus aureus, pseudomonas aeruginosa, klobsinella aerogenes and chromobacterium violaceum and antifungal activity against candida albicans, aspergillus fumigatus, trich ... | 2010 | 24061822 |
| 7,8- and 5,8-linoleate diol synthases support the heterolytic scission of oxygen-oxygen bonds by different amide residues. | linoleate diol synthases (lds) are fungal dioxygenase-cytochrome p450 fusion enzymes. they oxidize 18:2n-6 sequentially to 8r-hydroperoxylinoleic acid (8r-hpode) and 7s,8s- or 5s,8r-dihydroxylinoleic acids (dihode) by intramolecular oxygen transfer. the p450 domains contain a conserved sequence, ala-asn-gln-xaa-gln, presumably located in the i-helices. the asn938leu replacement of 7,8-lds of gaeumannomyces graminis virtually abolished and the asn938asp and asn938gln replacements reduced the hydr ... | 2013 | 24060467 |
| microwave assisted synthesis of dihydrobenzo[4,5]imidazo[1,2-a]pyrimidin-4-ones; synthesis, in vitro antimicrobial and anticancer activities of novel coumarin substituted dihydrobenzo[4,5]imidazo[1,2-a]pyrimidin-4-ones. | the present article describes the synthesis of dihydrobenzo[4,5]imidazo[1,2-a]pyrimidin-4-one (2a-h) under microwave irradiation. the product was obtained in excellent yield (74-94%) in a shorter reaction time (2 min). these molecules (2a, b) further reacted with various substituted 4-bromomethylcoumarins (3a-f) to yield a new series of coumarin substituted dihydrobenzo[4,5]imidazo[1,2-a]pyrimidin-4-ones (4a-h). the structure of all the synthesized compounds were confirmed by spectral studies an ... | 2013 | 24056147 |
| oxidative burst and neutrophil elastase contribute to clearance of aspergillus fumigatus pneumonia in mice. | polymorphonuclear neutrophils (pmn) are important for the control of invasive aspergillosis (ia), a major threat to immunocompromised individuals. for clearance of aspergillus fumigatus infections, pmn employ their potent oxidative and non-oxidative mechanisms. to clarify the relative contribution of these mechanisms, we analyzed p47(phox-/-), gp91(phox-/-) and elastase (ela) deficient mice (elane) after intratracheal infection with a. fumigatus. infected p47(phox-/-) and gp91(phox-/-) mice died ... | 2014 | 24054721 |
| antifungal activity of lactic acid bacteria isolated from kimchi against aspergillus fumigatus. | more than 120 isolates of lactic acid bacteria obtained from kimchi was screened for antifungal activity against aspergillus fumigatus. approximately 10% of the isolates showed inhibitory activity and only 4.16% (five isolates) exhibited strong activity against the indicator fungus a. fumigatus. the five isolates showed a wide rang of antifungal activity against a. flavus, fusarium moniliforme, penicillium commune, and rhizopus oryzae. they were identified by 16s rdna sequencing as lactobacillus ... | 2005 | 24049503 |
| death causes and pathogens analysis of systemic lupus erythematosus during the past 26 years. | different causes of mortality have been described over different decades followed by description of pathogens identified from infective episodes that led to death. a retrospective review was performed in 3,831 hospitalized systemic lupus erythematosus (sle) patients in peking union medical college hospital from january 1986 to april 2012. the primary causes of death were identified, and the constituent ratio of specific death causes during different periods was compared. among 3,831 hospitalized ... | 2014 | 24046218 |
| use of mycological, nested pcr, and real-time pcr methods on bal fluids for detection of aspergillus fumigatus and a. flavus in solid organ transplant recipients. | invasive aspergillosis continues to be a significant cause of morbidity and mortality in solid organ transplant (sot) recipients. a reliable and early diagnostic method is needed to improve survival. in this study, four methods direct microscopy, culture, nested pcr on internal transcribed spacer region, and taqman real-time pcr targeted β-tubulin gene were examined for the detection of aspergillus fumigatus and a. flavus in sixty-four bronchoalveolar lavage (bal) fluids that were obtained from ... | 2013 | 24045934 |
| signaling mechanism for aspergillus fumigatus tolerance in corneal fibroblasts induced by lps pretreatment. | tlrs, particularly tlr2 and tlr4, play primary roles in inflammatory responses triggered by aspergillus fumigatus and lead to the activation of signaling pathways that initiate host defense responses. we previously demonstrated that lps, a ligand of tlr4, can induce tolerance of a. fumigatus hyphae in telomerase-immortalized human stroma fibroblasts (thsfs). in the present study we investigated the role of tlr4, tlr2 and their downstream signaling pathways in this activity. the thsfs were pretre ... | 2014 | 24045340 |
| development of allergic bronchopulmonary aspergillosis with central bronchiectasis over a 10-year period: the need to recheck allergen sensitization. | a 55-year-old woman visited our hospital for an investigation of central bronchiectasis, mucoid impaction and infiltrative shadows on chest ct. she had a 10-year history of bronchial asthma; however, her adherence to treatment was poor. based on the presence of peripheral blood eosinophilia and immediate cutaneous reactivity to aspergillus fumigatus, the patient was clinically diagnosed with allergic bronchopulmonary aspergillosis. her condition and ct findings improved with systemic corticoster ... | 2013 | 24042528 |
| multidrug-resistant transporter mdr1p-mediated uptake of a novel antifungal compound. | the activity of many anti-infectious drugs has been compromised by the evolution of multidrug-resistant (mdr) pathogens. for life-threatening fungal infections, such as those caused by candida albicans, overexpression of mdr1, which encodes an mdr efflux pump of the major facilitator superfamily (mfs), often confers resistance to chemically unrelated substances, including the most commonly used azole antifungals. as the development of new and efficacious antifungals has lagged far behind the gro ... | 2013 | 24041896 |
| in vitro susceptibility of aspergillus fumigatus to isavuconazole: correlation with itraconazole, voriconazole, and posaconazole. | triazoles are first-line agents for treating aspergillosis. the prevalence of azole resistance in aspergillus fumigatus is increasing, and cross-resistance is a growing concern. in this study, the susceptibilities of 40 a. fumigatus clinical isolates were tested by using the clsi method with amphotericin b, itraconazole, voriconazole, posaconazole, and the new triazole isavuconazole. isavuconazole mics were higher in strains with reduced susceptibilities to other triazoles, mirroring changes in ... | 2013 | 24041890 |
| environmental monitoring for aspergillus fumigatus in association with an immunosuppressed rabbit model of pulmonary aspergillosis. | aspergillus fumigatus causes life-threatening pneumonia in immunocompromised patients. conidia, the infectious form of the organism, are handled in a biologic safety cabinet under bsl2 conditions. however because germinated conidia form noninfectious hyphae in tissue, we hypothesized that rabbits inoculated intratracheally would grow a. fumigatus in their lungs but that the environment would remain free of this fungus, potentially permitting maintenance of infected animals under absl1 conditions ... | 2013 | 24041208 |
| oxygen and an extracellular phase transition independently control central regulatory genes and conidiogenesis in aspergillus fumigatus. | conidiogenesis is the primary process for asexual reproduction in filamentous fungi. as the conidia resulting from the conidiogenesis process are primarily disseminated via air currents and/or water, an outstanding question has been how fungi recognize aerial environments suitable for conidial development. in this study, we documented the somewhat complex development of the conidia-bearing structures, termed conidiophores, from several aspergillus species in a subsurface (gel-phase) layer of sol ... | 2013 | 24040343 |
| epidithiodiketopiperazine biosynthesis: a four-enzyme cascade converts glutathione conjugates into transannular disulfide bridges. | enzyme quartet: isolation of the first sulfur-bearing intermediate of the gliotoxin pathway in aspergillus fumigatus and successful in vitro conversion of the bisglutathione adduct into an intact epidithiodiketopiperazine by a four-enzyme cascade (including glutamyltransferase glik and dipeptidase glij) revealed an outstanding adaptation of a primary metabolic pathway into natural product biosynthesis that is widespread in fungi. | 2013 | 24039048 |
| aspergillus fumigatus sidj mediates intracellular siderophore hydrolysis. | siderophore-mediated iron handling is crucial for the virulence of aspergillus fumigatus. here we identified a new component of its siderophore metabolism, termed sidj, which is encoded by afua_3g03390. the encoding gene is localized in a siderophore biosynthetic gene cluster that is conserved in a variety of fungi. during iron starvation, sidj deficiency resulted in decreased growth and increased intracellular accumulation of hydrolysis products of the siderophore fusarinine c. the implied role ... | 2013 | 24038704 |
| mixed infection of an atypical mycobacterium and aspergillus following a cryopreserved fat graft to a face. | we report a chronic infection of a patient who received a cryopreserved fat graft on her face. a 22-year-old female patient presented with multiple abscesses of her face. four months previously, she received a second fat graft with the fat harvested at a previous surgery which was cryopreserved for 2 months. on examination, she had tender erythematous nodules on both cheeks. a computed tomography of her neck showed multiple peripheral enhancing nodular lesions. in an open pus fungus culture, asp ... | 2013 | 24036751 |
| dexamethasone enhances invasiveness of aspergillus fumigatus conidia and fibronectin expression in a549 cells. | the efficacies of current treatments for invasive aspergillus (ia) are unsatisfactory and new therapeutic targets or regimens to treat ia are urgently needed. previous studies have indicated that the ability of conidia to invade host cells is critical in ia development and fibronectin has a hand in the conidia adherence process. in the clinical setting, many patients who receive glucocorticoid for extended periods are susceptible to aspergillus fumigatus (a. fumigatus) infection, for this reason ... | 2013 | 24033951 |
| pneumonia relevant to lung transplantation and pathogen distribution. | pneumonia is the most common cause of morbidity and mortality in lung transplant (lt) recipients. the aim of the present study was to evaluate the incidence, etiology, risk factors and prognosis of pneumonia in lt recipients. | 2013 | 24033938 |
| selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation. | twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45°c on medium containing corn straw and cardboard as carbon sources. these fungi were identified in the genera aspergillus, thermomyces, myceliophthora, thermomucor and candida. the majority of the isolated strains produced xylanase and cellulases under solid state fermentation (ssf). the highest cellulase and xylanase productions wer ... | 2012 | 24031929 |
| antifungal activity of different neem leaf extracts and the nimonol against some important human pathogens. | this study was conducted to evaluate the effect of aqueous, ethanolic and ethyl acetate extracts from neem leaves on growth of some human pathogens (aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus terreus, candida albicans and microsporum gypseum) in vitro. different concentrations (5, 10, 15 and 20%) prepared from these extracts inhibited the growth of the test pathogens and the effect gradually increased with concentration. the 20% ethyl acetate extract gave the stron ... | 2011 | 24031718 |
| the potential inhibitory effect of cuminum cyminum, ziziphora clinopodioides and nigella sativa essential oils on the growth of aspergillus fumigatus and aspergillus. | the goals of this study were to evaluate the effectiveness of cuminum cyminum, ziziphora clinopodioides and nigella sativa essential oils to inhibit the growth of aspergillus fumigatus and a. flavus and to evoke ultrastructural changes. the fungi were cultured into rpmi 1640 media in the presence of oils at concentrations of 8, 6, 5, 4, 3, 2, 1.5, 1.25, 1, 0.75 and 0.5 mg/ml in broth microdilution and 2, 1.5, 1 and 0.5 mg/ml in broth macrodilution methods with shaking for 48 h at 28(o)c. conidia ... | 2011 | 24031624 |
| microbial diversity in a bagasse-based compost prepared for the production of agaricus brasiliensis. | edible mushrooms are renowned for their nutritional and medicinal properties and are thus of considerable commercial importance. mushroom production depends on the chemical composition of the basic substrates and additional supplements employed in the compost as well as on the method of composting. in order to minimise the cost of mushroom production, considerable interest has been shown in the use of agro-industrial residues in the preparation of alternative compost mixtures. however, the inter ... | 2009 | 24031404 |
| invasive aspergillosis associated with systemic lupus erythematosus and cardiac postoperative complication. | aspergillus is a ubiquitous fungus which can cause a variety of clinical syndromes. this fungus has emerged as agent of systemic infections and has therefore gained considerable public health importance. this paper describes two cases of invasive aspergillosis caused by a. fumigatus in immuno-suppressed patients and underscores the importance of early identification of aspergillus infection associated with systemic lupus erythematosus and cardiac postoperative complications. | 2009 | 24031340 |
| discrimination of aspergillus lentulus from aspergillus fumigatus by raman spectroscopy and maldi-tof ms. | in 2005, a new sibling species of aspergillus fumigatus was discovered: aspergillus lentulus. both species can cause invasive fungal disease in immune-compromised patients. the species are morphologically very similar. current techniques for identification are pcr-based or morphology-based. these techniques are labour-intense and not sufficiently discriminatory. since a. lentulus is less susceptible to several antifungal agents, it is important to correctly identify the causative infectious agen ... | 2014 | 24030717 |
| comparison of methods for isolation of bacterial and fungal dna from human blood. | the study aimed at optimization of dna isolation from blood of representatives of four microbial groups causing sepsis, i.e., gram negative: escherichia coli, gram positive: staphylococcus aureus, yeast: candida albicans, and filamentous fungus: aspergillus fumigatus. additionally, the five commercial kits for microbial dna isolation from the blood were tested. the developed procedure of dna isolation consisted of three consecutive steps, i.e., mechanical disruption, chemical lysis, and thermal ... | 2014 | 24026449 |
| extensive proteomic remodeling is induced by eukaryotic translation elongation factor 1bγ deletion in aspergillus fumigatus. | the opportunistic pathogen aspergillus fumigatus is ubiquitous in the environment and predominantly infects immunocompromised patients. the functions of many genes remain unknown despite sequencing of the fungal genome. a putative translation elongation factor 1bγ (eef1bγ, termed elfa; 750 bp) is expressed, and exhibits glutathione s-transferase activity, in a. fumigatus. here, we demonstrate the role of elfa in the oxidative stress response, as well as a possible involvement in translation and ... | 2013 | 24023013 |
| biological screening of a novel nickel (ii) tyrosine complex. | a newly synthesized nickel (ii) tyrosine complex was screened as potential antimicrobial agent against a number of medically important bacteria (bacillus subtilis, streptococcus β-haemolytica, escherichia coli, shigella dysenterae) and fungi (aspergillus fumigatus, candida albicans, aspergillus niger, aspergillus flavus, penicillium sp.) strains. were used for antifungal activity. the antimicrobial activity was evaluated using the agar disc method. moreover, the minimum inhibitory concentration ... | 2007 | 24015064 |
| new insights concerning the occurrence of fungi in water sources and their potential pathogenicity. | fungi are known to occur ubiquitously in the environment. in the past years, the occurrence of filamentous fungi in the aquatic environment has been a subject of growing interest. this study describes the occurrence of various fungal genera in different drinking water sources being penicillium and trichoderma the most representative ones (30% and 17%, respectively). also, 24 fungal species that have not been previously described in the aquatic environment are reported in this study, being once a ... | 2013 | 24011405 |
| comparative evaluation of galactomannan optical density indices and culture results in bronchoscopic specimens obtained from neutropenic and non-neutropenic patients. | aspergillus infections are major causes of morbidity and mortality among immunocompromised patients. this study was designed to investigate the galactomannan assay optical density (od) indices relative to the culture results in bronchoscopic samples obtained from neutropenic and non-neutropenic patients. galactomannan od indices from 1427 samples from 2005 to 2012, which were sent from 839 patients and were composed of bronchial lavage (bl = 727) and bronchoalveolar lavage fluids (bal = 700), we ... | 2014 | 24010950 |
| regulation of sulphur assimilation is essential for virulence and affects iron homeostasis of the human-pathogenic mould aspergillus fumigatus. | sulphur is an essential element that all pathogens have to absorb from their surroundings in order to grow inside their infected host. despite its importance, the relevance of sulphur assimilation in fungal virulence is largely unexplored. here we report a role of the bzip transcription factor metr in sulphur assimilation and virulence of the human pathogen aspergillus fumigatus. the metr regulator is essential for growth on a variety of sulphur sources; remarkably, it is fundamental for assimil ... | 2013 | 24009505 |
| seasonal variation in skin sensitivity to aeroallergens. | we previously demonstrated seasonal variation in sensitization to aeroallergens in a small group of patients with exercise-induced asthma. this study was performed to confirm the relationship in a much larger population. | 2013 | 24003387 |
| exacerbation of allergen-induced eczema in tlr4- and trif-deficient mice. | despite its presence on resident skin cells, the role of tlr4 in skin diseases remains poorly understood. this is highly significant because the skin biome is rich with potential tlr4 agonists. we aimed to establish the contribution of tlr4 to atopic dermatitis and determine the mechanism by which tlr4 acts in an experimental model of atopic dermatitis. myd88, tlr4, or toll-il-1r domain-containing adapter-inducing ifn-β (trif)-deficient and wild-type mice were epicutaneously exposed to aspergill ... | 2013 | 23997219 |
| ca2+-dependent phospholipid scrambling by a reconstituted tmem16 ion channel. | phospholipid (pl) scramblases disrupt the lipid asymmetry of the plasma membrane, externalizing phosphatidylserine to trigger blood coagulation and mark apoptotic cells. recently, members of the tmem16 family of ca(2+)-gated channels have been shown to be involved in ca(2+)-dependent scrambling. it is however controversial whether they are scramblases or channels regulating scrambling. here we show that purified aftmem16, from aspergillus fumigatus, is a dual-function protein: it is a ca(2+)-gat ... | 2013 | 23996062 |
| invariant natural killer t cells recognize a fungal glycosphingolipid that can induce airway hyperreactivity. | aspergillus fumigatus is a saprophytic fungus that is ubiquitous in the environment and is commonly associated with allergic sensitization and severe asthma in humans. although a. fumigatus is recognized by multiple microbial pattern-recognition receptors, we found that an a. fumigatus-derived glycosphingolipid, asperamide b, directly activates invariant natural killer t (inkt) cells in vitro in a cd1d-restricted, myd88-independent and dectin-1-independent fashion. moreover, asperamide b, when l ... | 2013 | 23995283 |
| aspergillus flavus velb acts distinctly from vea in conidiation and may coordinate with flug to modulate sclerotial production. | the proteins vea, velb and laea of aspergillus nidulans form a heterotrimeric complex (the velvet complex) in the dark to coordinate sexual development and production of some secondary metabolites. vea and velb of a. nidulans and aspergillus fumigatus also are repressors of conidiation, but vea of aspergillus flavus in studied strains acts positively on conidiation. in the present study, we show via yeast-two hybrid assays that interactions among a. flavus vea, velb, and laea are conserved as in ... | 2013 | 23994319 |
| composite survival index to compare virulence changes in azole-resistant aspergillus fumigatus clinical isolates. | understanding resistance to antifungal agents in aspergillus fumigatus is of increasing importance for the treatment of invasive infections in immunocompromised patients. although a number of molecular resistance mechanisms are described in detail, the potential accompanying virulence changes and impact on clinical outcome have had little attention. we developed a new measure of survival, the composite survival index (csi) to use as a measure of the virulence properties of a. fumigatus. using a ... | 2013 | 23991080 |
| aspergillus galactosaminogalactan mediates adherence to host constituents and conceals hyphal β-glucan from the immune system. | aspergillus fumigatus is the most common cause of invasive mold disease in humans. the mechanisms underlying the adherence of this mold to host cells and macromolecules have remained elusive. using mutants with different adhesive properties and comparative transcriptomics, we discovered that the gene uge3, encoding a fungal epimerase, is required for adherence through mediating the synthesis of galactosaminogalactan. galactosaminogalactan functions as the dominant adhesin of a. fumigatus and med ... | 2013 | 23990787 |
| phosphorylation of calcineurin at a novel serine-proline rich region orchestrates hyphal growth and virulence in aspergillus fumigatus. | the fungus aspergillus fumigatus is a leading infectious killer in immunocompromised patients. calcineurin, a calmodulin (cam)-dependent protein phosphatase comprised of calcineurin a (cnaa) and calcineurin b (cnab) subunits, localizes at the hyphal tips and septa to direct a. fumigatus invasion and virulence. here we identified a novel serine-proline rich region (sprr) located between two conserved cnaa domains, the cnab-binding helix and the cam-binding domain, that is evolutionarily conserved ... | 2013 | 23990785 |
| dissociation between sensitizing and colonizing fungi in patients with allergic bronchopulmonary aspergillosis. | because allergic bronchopulmonary aspergillosis (abpa) does not require the presence of aspergillus fumigatus for diagnosis, serological and radiological findings without cultures usually confirm this condition. | 2013 | 23987194 |
| the immune interplay between the host and the pathogen in aspergillus fumigatus lung infection. | the interplay between aspergillus fumigatus and the host immune response in lung infection has been subject of studies over the last years due to its importance in immunocompromised patients. the multifactorial virulence factors of a. fumigatus are related to the fungus biological characteristics, for example, structure, ability to grow and adapt to high temperatures and stress conditions, besides capability of evading the immune system and causing damage to the host. in this context, the fungus ... | 2013 | 23984400 |
| aspergillus tracheobronchitis causing subtotal tracheal stenosis in a liver transplant recipient. | invasive aspergillosis is recognized as one of the most significant opportunistic infections after liver transplantation. diagnosis of invasive aspergillosis in transplant recipients has been proven to be challenging, and optimal approach to the treatment of invasive aspergillosis is still controversial. we here present an unusual case of aspergillus tracheobronchitis in the setting of liver transplantation. a 47-year-old female patient with persistent dry cough after liver transplantation devel ... | 2013 | 23984170 |
| inhibition of conidiophore development in aspergillus fumigatus by an escherichia coli dh5α strain, a promising antifungal candidate against aspergillosis. | the opportunistic human pathogen aspergillus fumigatus produces a massive number of asexual spores (conidia) as the primary means of dispersal, survival, genome protection and infection of hosts. in this report, we investigated secretory and cytosolic proteins of non-pathogenic bacterial species (mostly belonging to human microbiome) for antifungal potential against a. fumigatus, a. flavus and a. niger. our preliminary results revealed that cytosolic proteins of e. coli dh5α were most active and ... | 2014 | 23978655 |
| allergic bronchopulmonary aspergillosis in garden waste (compost) collectors--occupational implications. | the separation of rotting garden material from general domestic waste and its collection for processing in industrial composting sites is a relatively new industry in the uk. two cases of allergic bronchopulmonary aspergillosis and the results of health surveillance are described in a team of 28 garden waste (compost) collectors. a few cases of extrinsic allergic alveolitis due to aspergillus fumigatus have previously been reported in compost workers. in the absence of any guidance from research ... | 2013 | 23975883 |
| bimodular peptide synthetase side produces fumarylalanine in the human pathogen aspergillus fumigatus. | the filamentous mold aspergillus fumigatus causes invasive aspergillosis, a potentially life-threatening infectious disease, in humans. the side gene encodes a bimodular peptide synthetase and was shown previously to be strongly upregulated during initiation of murine lung infection. in this study, we characterized the two adenylation domains of side with the atp-[(32)p]pyrophosphate exchange assay in vitro, which identified fumarate and l-alanine, respectively, as the preferred substrates. usin ... | 2013 | 23974138 |
| identification and quantification of fungi and mycotoxins from pu-erh tea. | pu-erh tea originates from the province of yunnan in south-western china. as this tea is produced by so called aspergillus post-fermentation the question arises which molds and mycotoxins may be found in this tea. in total 36 samples of pu-erh tea were investigated for their content of filamentous fungi and the mycotoxins aflatoxins b1, b2, g1, and g2, fumonisins b1, b2, and b3, and ochratoxin a. fungi were isolated from all samples in a concentration of 1.0×10(1) to 2.6×10(6) colony forming uni ... | 2013 | 23973844 |
| acute chiasmal abscess resulting from perineural extension of invasive sino-orbital aspergillosis in an immunocompetent patient. | intracranially invasive sino-orbital aspergillosis is a rare entity seen predominantly in immunocompromised individuals. we report a unique case of an acute chiasmal abscess resulting from perineural extension of an indolent invasive sino-orbital aspergillosis in an immunocompetent patient. | 2014 | 23973515 |
| effect of hay dust extract and cyathostomin antigen stimulation on cytokine expression by pbmc in horses with recurrent airway obstruction. | equine recurrent airway obstruction (rao) is an inflammatory, obstructive airway disease induced by exposure of susceptible horses to inhaled organic dust particles. the immunological process underlying rao is still unclear. previous studies have shown that rao is linked to the interleukin-4 receptor (il-4r) gene in one warmblood family (f1), but not in another (f2). it has also been shown that in f1, but not in f2, rao is associated with resistance against parasites, suggesting that this associ ... | 2013 | 23972861 |
| primary cutaneous aspergillosis due to aspergillus tamarii in an immunocompetent host. | primary cutaneous aspergillosis is a rare disease usually caused by aspergillus fumigatus, aspergillus flavus, aspergillus terreus and aspergillus ustus. it is usually seen in immunocompromised hosts, though some cases are also reported in immunocompetent hosts. we present a case of an immunocompetent farmer who presented with generalised nodules and plaques, mimicking erythema nodosum leprosum but turned out to be cutaneous aspergillosis caused by aspergillus tamarii. the characteristic ascospo ... | 2013 | 23970496 |
| an anti-aspergillus protein from escherichia coli dh5α: putative inhibitor of siderophore biosynthesis in aspergillus fumigatus. | an antifungal protein designated as anti-aspergillus protein (aap), produced by escherichia coli dh5α, was purified and characterised. it exhibited a molecular weight of 60 kda on sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis and depicted 99% purity on ultra performance liquid chromatography. the purified protein manifested antimycotic potential against pathogenic isolates of aspergillus spp., depicting a minimum inhibitory concentration in the range 15.62-31.25 μg ml(-1) a ... | 2014 | 23968167 |
| the crucial role of iron uptake in aspergillus fumigatus virulence. | aspergillus fumigatus is an opportunistic fungal pathogen that causes life-threatening infections in immunocompromised individuals. siderophore-mediated iron acquisition has been shown to be essential for virulence. new studies have revealed that enzymes involved in siderophore biosynthesis and uptake are compartmentalized in peroxisomes and endosome-like vesicles, respectively. gene and protein expression studies have revealed coordinated regulation of siderophore and sterol metabolism linked t ... | 2013 | 23962820 |
| biofilm formation by aspergillus fumigatus. | aspergillus fumigatus is a well adapted, opportunistic fungus that causes a severe and commonly fatal disease, invasive pulmonary aspergillosis (ipa), in highly immunocompromised patients, aspergilloma in patients with lung cavities and allergic bronchopulmonary aspergillosis (abpa) in hypersensitive individuals. recent studies have suggested that biofilm formation by a. fumigatus may be one of the most important virulence factors in ipa and aspergilloma. several fungal constituents may contribu ... | 2014 | 23962172 |
| synthesis, characterization, and antimicrobial evaluation of carbostyril derivatives of 1h-pyrazole. | a new series of 12 derivatives of 4-pyrazolyl-n-arylquinoline-2,5-dione (4a-l) were synthesized by one pot base catalyzed cyclocondensation reaction of 1-aryl-5-chloro-3-methyl-1h-pyrazole-4-carbaldehyde (1a-c), meldrum's acid (2) and 3-arylamino-5,5-disubstitutedcyclohex-2-enone (3a-d). all the compounds were characterized by elemental analysis, ft-ir, (1)h nmr and (13)c nmr spectral data and were screened, against six bacterial pathogens, namely bacillus subtilis, clostridium tetani, streptoco ... | 2011 | 23960745 |
| impact of in vivo triazole and echinocandin combination therapy for invasive pulmonary aspergillosis: enhanced efficacy against cyp51 mutant isolates. | previous studies examining combination therapy for invasive pulmonary aspergillosis (ipa) have revealed conflicting results, including antagonism, indifference, and enhanced effects. the most commonly employed combination for this infection includes a mold-active triazole and echinocandin. few studies have evaluated combination therapy from a pharmacodynamic (pd) perspective, and even fewer have examined combination therapy against both wild-type and azole-resistant cyp51 mutant isolates. the cu ... | 2013 | 23959322 |
| determination of isavuconazole susceptibility of aspergillus and candida species by the eucast method. | isavuconazole is a novel expanded-spectrum triazole, which has recently been approved by the fda as an orphan drug to treat invasive aspergillosis and is currently being studied in phase iii clinical trials for invasive candidiasis. the susceptibility of relatively few clinical isolates has been reported. in this study, the isavuconazole susceptibilities of 1,237 aspergillus and 2,010 candida geographically diverse clinical isolates were determined by eucast methodology at four european mycology ... | 2013 | 23959309 |
| regulation of development in aspergillus nidulans and aspergillus fumigatus. | members of the genus aspergillus are the most common fungi and all reproduce asexually by forming long chains of conidiospores (or conidia). the impact of various aspergillus species on humans ranges from beneficial to harmful. for example, several species including aspergillus oryzae and aspergillus niger are used in industry for enzyme production and food processing. in contrast, aspergillus flavus produce the most potent naturally present carcinogen aflatoxins, which contaminate various plant ... | 2010 | 23956662 |
| isolation and identification of aspergillus section fumigati strains from arable soil in korea. | 63 strains of aspergillus section fumigati were isolated from 17 samples of arable soil in a central province of korea. based on the results of genotypic and phenotypic analyses, they were identified as aspergillus fumigatus, a. lentulus, neosartorya coreana, n. fennelliae, n. fischeri, n. glabra, n. hiratsukae, n. laciniosa, n. pseudofischeri, n. quadricincta, n. spinosa and n. udagawae. among these, n. fennelliae, n. hiratsukae, n. quadricincta, and n. udagawae had not been previously recorded ... | 2010 | 23956617 |
| outcome of tini stent treatments in symptomatic central airway stenoses caused by aspergillus fumigatus infections after lung transplantation. | central airway stenoses due to aspergillus fumigatus infections have been a significant cause of morbidity and mortality after lung transplantation. we reviewed our experience using self-expandable braided tini-metallic stents in the management of 4 single-lung transplant recipients with central airways stenoses between january 2003 and june 2010. | 2013 | 23953550 |
| anterior and pan-endophthalmitis caused by candida albicans and aspergillus fumigatus: report of two cases. | we report two posttraumatic fungal endophthalmitis cases with anterior and entire segment involvement caused, respectively, by candida albicans and aspergillus fumigatus. the anterior endophthalmitis induced by c. albicans presented multifocal lesions with hyphael structures in anterior chamber, but without involvement of posterior vitreous. the pan-endophthalmitis caused by a. fumigatus displayed significant hypopyon and vitritis. treatment strategies are anterior chamber cleaning for anterior ... | 2013 | 23948966 |
| an inhalation model of allergic fungal asthma: aspergillus fumigatus-induced inflammation and remodeling in allergic airway disease. | the ability to accurately mimic normal processes for sensitization and allergen challenge in an experimental animal model are useful in that they allow researchers to critically manipulate the complex interactions of multiple cell types. in the context of the allergic lung, multiple cell types form complex cellular networks and function to regulate a variety of temporal and spatial changes. mouse models of allergic airway disease have proven to be highly useful for dissecting these complex inter ... | 2013 | 23943453 |
| microcalorimetry assay for rapid detection of voriconazole resistance in aspergillus fumigatus. | we describe a calorimetric assay for detection of voriconazole-resistant aspergillus fumigatus within 8 h. among 27 genetically distinct strains, all 21 resistant and all 6 susceptible strains were correctly identified by measurement of fungal heat production in the presence of voriconazole. this proof-of-concept study demonstrates the potential of microcalorimetry for rapid detection of azole resistance in a. fumigatus. | 2013 | 23939893 |
| antifungal cyclic peptides from the marine sponge microscleroderma herdmani. | screening natural product extracts from the national cancer institute open repository for antifungal discovery afforded hits for bioassay-guided fractionation. using lc-ms analysis to generate chemical structure information on potentially active compounds, two new cyclic hexapeptides, microsclerodermins j (1) and k (2), were isolated from the deep-water sponge microscleroderma herdmani, along with microsclerodermins a (3) and b (4), previously isolated from an unidentified microscleroderma speci ... | 2012 | 23936761 |
| gβ-like cpcb plays a crucial role for growth and development of aspergillus nidulans and aspergillus fumigatus. | growth, development, virulence and secondary metabolism in fungi are governed by heterotrimeric g proteins (g proteins). a gβ-like protein called gib2 has been shown to function as an atypical gβ in gpa1-camp signaling in cryptococcus neoformans. we found that the previously reported cpcb (cross pathway control b) protein is the ortholog of gib2 in aspergillus nidulans and aspergillus fumigatus. in this report, we further characterize the roles of cpcb in governing growth, development and toxige ... | 2013 | 23936193 |
| t-2 toxin impairs antifungal activities of chicken macrophages against aspergillus fumigatus conidia but promotes the pro-inflammatory responses. | aspergillosis is the most common fungal disease of the avian respiratory tract and is caused primarily by aspergillus fumigatus. the respiratory macrophages provide important defence against aspergillosis. t-2 toxin (t-2), a trichothecene mycotoxin produced by fusarium spp. in improperly stored agricultural products, has immunomodulatory effects. we studied the impact of t-2 on the antifungal response of the chicken macrophage cell line hd-11 against a. fumigatus infection. the macrophages were ... | 2013 | 23930935 |
| dectin-2 is predominately macrophage restricted and exhibits conspicuous expression during aspergillus fumigatus invasion in human lung. | we investigated the features of dectin-2 expression both at transcriptional and translational levels during aspergillus fumigatus infection in human lung. simultaneously, the expression of cd206 was assayed as an activated marker of alveolar macrophages. the characteristic of dectin-2 expression were then confirmed in monocyte-derived macrophages (mdm) after a. fumigatus stimulation by flow cytometry. we found that the expression of dectin-2 was low in normal lung, while it revealed a markedly u ... | 2013 | 23928558 |
| different colonization patterns of aspergillus terreus in patients with cystic fibrosis. | aspergillus terreus is a common soil saprophyte. after aspergillus fumigatus and scedosporium apiospermum it ranks third amongst the filamentous fungi colonizing the airways of patients with cystic fibrosis. in this context, the clinical presentation of a. terreus infection mainly corresponds to allergic broncho-pulmonary aspergillosis. in the work presented here, we studied colonization patterns of a. terreus in cf patients by genotyping using nine short tandem repeat markers. a total of 115 cl ... | 2014 | 23927682 |
| the roda hydrophobin on aspergillus fumigatus spores masks dectin-1- and dectin-2-dependent responses and enhances fungal survival in vivo. | aspergillus and fusarium species are important causes of fungal infections worldwide. airborne spores (conidia) of these filamentous fungi express a surface protein that confers hydrophobicity (hydrophobin) and covers cell wall components that would otherwise induce a host immune cell response. using a mutant aspergillus fumigatus strain (δroda) that does not express the roda hydrophobin, and aspergillus and fusarium conidia from clinical isolates that were treated with hydrofluoric acid (which ... | 2013 | 23926321 |
| accumulation of ergot alkaloids during conidiophore development in aspergillus fumigatus. | production of ergot alkaloids in the opportunistic fungal pathogen aspergillus fumigatus is restricted to conidiating cultures. these cultures typically accumulate several pathway intermediates at concentrations comparable to that of the pathway end product. we investigated the contribution of different cell types that constitute the multicellular conidiophore of a. fumigatus to the production of ergot alkaloid pathway intermediates versus the pathway end product, fumigaclavine c. a relatively m ... | 2014 | 23925951 |