Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
[identification of genes for biofilm formation in a salmonella enteritidis strain by transposon mutagenesis].biofilm plays an important role in pathogenicity of salmonella and food poisoning caused by salmonella. our aim was to identify genes associated with salmonella biofilm formation.200818837362
fluorescent bio-barcode dna assay for the detection of salmonella enterica serovar enteritidis.salmonella enterica serovar enteritidis is one of the most frequently reported causes of foodborne illness. it is a major threat to the food safety chain and public health. a highly amplified bio-barcode dna assay for the rapid detection of the insertion element (iel) gene of salmonella enteritidis is reported in this paper. the biosensor transducer is composed of two nanoparticles: gold nanoparticles (au-nps) and magnetic nanoparticles (mnps). the au-nps are coated with the target-specific dna ...200918835708
anti-inflammatory effects of the methanol extract of sedum telephium ssp. maximum in lipopolysaccharide- stimulated rat peritoneal macrophages.sedum telephium ssp. maximum is a medicinal plant that possesses anti-inflammatory, analgesic and keratolytic properties. we investigated the anti-inflammatory activity of its methanolic extract (stme) in rat peritoneal macrophages (mphis) stimulated with lipopolysaccharide from salmonella enteritidis. after stimulation with 10 microg/ml of lps, mphis were coincubated with different doses of stme (8, 16 and 32 microg/ml) or rpmi medium alone using different times of incubation. stme reduced leve ...200818818510
large salmonella enteritidis outbreak with prolonged transmission attributed to an infected food handler, texas, 2002.in march 2002, an outbreak of salmonella enteritidis (se) infections occurred at a convention centre in dallas, texas and continued for 6 weeks. we conducted epidemiological studies, obtained clinical and environmental cultures, and interviewed employees to identify risk factors for infection. from 17 march-25 april 2002, the implicated hotel kitchen catered 41 multi-day conferences attended by 9790 persons. we received 617 illness reports from residents of 46 states. sauces or items served with ...200918817586
high resolution, advanced intercross mapping of host resistance to salmonella colonization.availability of a dense single-nucleotide polymorphism (snp) map in chickens has allowed for whole-genome qtl mapping for disease resistance. in this study, two f8 advanced intercross lines of broiler by fayoumi and broiler by leghorn chickens, and a dense, genome-wide snp panel were used to map genomic regions associated with host resistance to bacterial colonisation. one week after inoculation of day-old chicks with salmonella enteritidis (se), caecum and spleen tissues were collected to quant ...200818817304
transcriptional response of chicken macrophages to salmonella enterica serovar enteritidis infection.the transcriptional profiles of chicken macrophages (hd11) infected with salmonella enterica serovar enteritidis (se) were analyzed by using an avian macrophage microarray and real time rt-pcr. out of 4906 array elements interrogated, 269 genes exhibited a 2 fold change (p < 0.001) over a 24 h time-course. genes coding for proinflammatory cytokines, cc and cxc chemokines, and chemokine ligand were upregulated; whereas genes associated with transcription, cell adhesion and proliferation were down ...200818817296
rapid detection of salmonella enteritidis by pcr amplification of the sefa gene and it's cloning.the emergence of salmonella enteritidis as an important food-borne pathogenesis in humans, demands the development of novel detection and intervention strategies. it is generally accepted that fimbriae are an important factor in bacterial survival and persistence in the host. this study is directed towards the method of amplifying and cloning the sefa gene, which encode salmonella enteritidis fimbrial protein. strains used for these studies were s. enteritidis (e3), which were collected from ker ...200818817167
multiplication of salmonella enteritidis on the yolk membrane and penetration to the yolk contents at 30 degrees c in an in vitro egg contamination model.refrigeration to limit bacterial multiplication is a critical aspect of efforts to control the transmission of salmonella enterica serovar enteritidis (se) to consumers of contaminated eggs. although the nutrient-rich yolk interior is an uncommon location for se contamination in freshly laid, naturally contaminated eggs, migration across the vitelline membrane could lead to rapid bacterial multiplication even when the initial site of deposition is outside the yolk. multiplication on the yolk mem ...200818810876
[salmonellosis in poland in 2006].in 2006, 13 362 salmonellosis cases were reported (16 006 in 2005), the incidence rate was 35.0 per 100 000 population. in total 298 outbreaks were reported involving 2 965 cases. the most common type of outbreaks were households outbreaks. over seventy percent of patients were hospitalized, however the hospitalization rate was higher for extraintestinal manifestations--94.7%. as in previous years the seasonal peak was observed in july and august. the most frequently isolated type remained salmo ...200818807472
[foodborne infections and intoxications in poland in 2006].a total of 17 157 bacterial foodborne infections and intoxications were registered in 2006 (incidence 45/100 000 population). unlike bacterial infections, the incidence of viral foodborne infections increased in the recent years (2006 - 54.21 vs. 2005 - 32.8, 2004 - 27.2, 2003 - 21.1 and median 1998-2002 - 10.0). the parasitic foodborne infections did not change much during the recent years. there were 11 deaths related to foodborne infections or intoxications registered in 2006, of which the fo ...200818807469
antimicrobial efficacy of citron essential oil on spoilage and pathogenic microorganisms in fruit-based salads.the aim of this study was the evaluation of the effects of pure citral and citron essential oil on microbial spoilage and growth and survival of pathogenic microorganisms during storage. they were added in the syrup of industrial ready-to-eat fruit salads stored at 9 degrees c. both citral (25 to 125 ppm) and citron essential oil (300, 600, 900 ppm) were able to prolong the microbial shelf life of the fruit-based salads. the essential oil gave excellent results, avoiding the undesirable effects ...200818803716
salmonella enterica serovar enteritidis dam mutant induces low nos-2 and cox-2 expression in macrophages via attenuation of mapk and nf-kappab pathways.although dam mutants of salmonella have been proposed as live vaccines, their capacity to trigger cell inflammatory cascades has not been fully elucidated. we investigated in detail the ability of salmonella enterica dam mutant to activate the signalling pathways of the inflammatory response in raw 264.7 cells. apoptosis in macrophages treated with salmonella dam mutant was low. similarly, the expression of both nos-2 and cox-2 and subsequently the production of no and pge(2) was significantly r ...200818801455
allele distribution and genetic diversity of vntr loci in salmonella enterica serotype enteritidis isolates from different sources.salmonella enterica serotype enteritidis (s. enteritidis) is a zoonotic pathogen, which can be found in many sources including animals and the environment. however, little is known about the molecular relatedness among s. enteritidis isolates from different sources. we have applied multiple-locus variable number tandem repeat analysis (mlva) to study the genetic diversity of s. enteritidis isolates from human and non-human sources.200818793420
replication kinetics of salmonella enteritidis in internal organs of ducklings after oral challenge: a quantitative time-course study using real-time pcr.this research was undertaken to understand the replication kinetics of salmonella enteritidis (s. enteritidis) in the internal organs of ducklings after oral challenge over a 2 wk period. a serovar-specific real-time, fluorescence-based quantitative polymerase chain reaction (fq-pcr) assay was used to detect genomic dna of s. enteritidis in the blood and the internal organs at different time points respectively. the results showed that the spleen was positive at 12 h post inoculation (pi) and th ...200918781393
salmonella enterica serovar enteritidis genes induced during oviduct colonization and egg contamination in laying hens.salmonella enterica serovar enteritidis is the predominant serovar associated with salmonellosis worldwide, which is in part due to its ability to contaminate the internal contents of the hen's egg. it has been shown that s. enterica serovar enteritidis has an unusual tropism for the avian reproductive tract and an ability to persist in the oviduct and ovary. factors allowing s. enterica serovar enteritidis strains to contaminate eggs could be a specific interaction with the oviduct tissue, lead ...200818776023
antifungal cyclic depsipeptide, eujavanicin a, isolated from eupenicillium javanicum.in the course of searching for new antifungal agents, a new cyclic depsipeptide, eujavanicin a (1), was isolated from eupenicillium javanicum as an antifungal agent against the human pathogenic filamentous fungus aspergillus fumigatus. the structure of 1 was established by spectroscopic and chemical investigations. the absolute stereochemistry was elucidated by marfey's method and by chiral hplc analysis.200818771240
a study on the variability in the growth limits of individual cells and its effect on the behavior of microbial populations.the nacl growth limits of individual salmonella enteritidis cells were studied. six different levels of the pathogen (from 10(1) to 10(6) cells) were inoculated on tryptic soy agar plates at nacl concentrations ranging from 0.5 to 8% and ph values of 5.0, 5.5 or 7.3. the nacl growth limits of individual cells were estimated from the ratio between the number of colonies formed at each nacl concentration interval and the number of cells initially inoculated, based on the assumption that each colon ...200818760853
the pathogenesis of salmonella enteritidis in experimentally infected ducks: a quantitative time-course study using taqman polymerase chain reaction.ducks were subcutaneously infected with a high-virulence strain of salmonella enterica ssp. enterica serovar enteritidis (salmonella enteritidis). the kinetics of the salmonella enteritidis genomic dna loads, the immunohistochemical localization of the bacterial antigens, and the histopathological examination in various tissues were investigated. the results showed that the time course of the appearance of the salmonella enteritidis bacterial antigens and the lesions in various tissues was coinc ...200818753444
influence of stressors on normal intestinal microbiota, intestinal morphology, and susceptibility to salmonella enteritidis colonization in broilers.in modern poultry production systems, environmental stressors may influence bird performance and susceptibility to pathogens such as salmonella enteritidis. experiments were conducted to determine the influence of 24-h feed withdrawal and 24-h exposure to high temperature (30 degrees c) on intestinal characteristics of broilers. attachment of salmonella enteritidis to ileal tissue was determined using an in vitro ileal loop assay. changes in commensal intestinal microbial populations were determ ...200818753440
antimicrobial activity of extracts of edible wild and cultivated mushrooms against foodborne bacterial strains.the antimicrobial activity of aqueous, methanol, hexane, and ethyl acetate extracts from edible wild and cultivated mushrooms against nine foodborne pathogenic bacterial strains (escherichia coli o157:h7, salmonella enteritidis, shigella sonnei, vibrio parahaemolyticus, yersinia enterocolitica, bacillus cereus, clostridium perfringens, listeria monocytogenes, and staphylococcus aureus) was screened with a disk diffusion assay. twenty-nine of the 48 species tested had antimicrobial activity. meth ...200818724768
effect of poultry decontaminants concentration on growth kinetics for pathogenic and spoilage bacteria.various chemical compounds are currently under review for final approval as poultry decontaminants in the european union (eu). concentration is among the factors considered by the eu authorities in the evaluation of these treatments. the aim of this research was to compare the growth parameters for pathogenic and spoilage bacteria in presence of high and low concentrations of poultry decontaminants to assess whether such treatments could involve a potential sanitary risk for consumers. growth cu ...200818721678
effects of salmonella enterica serovar enteritidis on cellular recruitment and cytokine gene expression in caecum of vaccinated chickens.although vaccination of poultry is a suitable method to limit human food borne gastroenteritis caused by salmonella (s.), the immune mechanisms responsible for a longer lasting protection against salmonella infection in birds are not completely understood. to reveal unique protection-related immune parameters, day-old chicks were vaccinated with a commercial live s. enteritidis vaccine and challenged with wild-type s. enteritidis 147n at day 56 of life. the bacterial cell count was determined in ...200818706948
development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1.we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ...200918706944
multiple-locus variable-number tandem repeat analysis for subtyping of salmonella enterica subsp. enterica serovar enteritidis.salmonella enterica subsp. enterica serovar enteritidis is a major food-borne pathogen that caused most of salmonella infections worldwide. s. enteritidis phage type 4 (pt4) especially presents a real challenge for the classical typing methods. we developed a simple multiple-locus variable-number tandem repeat analysis (mlva) assay based on three hypervariable variable-number tandem repeat (vntr) loci for subtyping of salmonella enteritidis. testing an arbitrary chosen strain collection of 110 s ...200918706857
lps-induced murine abortions require c5 but not c3, and are prevented by upregulating expression of the cd200 tolerance signaling molecule.lipopolysaccharide (lps) acts via tlr4 to promote th1 cytokine secretion and abortions. lps is an essential co-factor in spontaneous abortion in the cba x dba/2 model and in stress-triggered abortions. in the cba x dba/2 model, c3a, c5a, and fgl2 prothrombinase participate in triggering inflammation that terminates embryo viability. as fgl2 prothrombinase (via thrombin) can generate c5a, it was predicted that lps-driven abortions (which require fgl2) would be independent of c3. cd200fc can preve ...200818705840
salmonella tuboovarian abscess.a 38 year old lady with a previous history of an ovarian cyst, presented with a one week history of fever, diarrhoea and intense localized pain in the left iliac fossa. laparotomy revealed a left tuboovarian abscess with adherent bowels. pus from the ruptured cyst grew salmonella enteritidis. histologically the cyst wall showed haemorrhagic and degenerate endometriotic features. recovery was uneventful with cefotaxime and metronidazole.200718705483
essential genes in salmonella enteritidis as identified by tnaraout mutagenesis.tnaraout is a mariner-based transposon containing an arabinose-inducible promoter p(bad) facing outward. tnaraout mutagenesis previously used to identify essential genes in vibrio cholerae can also be used to identify in vitro essential genes in salmonella enteritidis. a mutant screen was conducted based on the assumption that a mutant-harboring tnaraout insertion in the promoter region of an essential gene should exhibit arabinose-dependent growth phenotype. among five isolated mutants with suc ...200818704577
reorganisation of the caecal extracellular matrix upon salmonella infection--relation between bacterial invasiveness and expression of virulence genes.interactions of salmonella (s.) outer membrane structures with extracellular matrix (ecm) of host tissues seem to be crucial for bacterial adhesion and invasion. to evaluate the relationship between the ecm and bacterial invasiveness, the reorganisation of fibronectin, tenascin-c and laminin after salmonella exposure in vivo, the salmonella adhesiveness to ecm proteins in vitro and the virulence gene expression upon co-cultivation of salmonellae and ecm proteins were elucidated for two salmonell ...200918692970
the use of two live attenuated vaccines to immunize egg-laying hens against salmonella enteritidis phage type 4.laying hens aged 24 weeks were vaccinated twice, separated by a 2-week interval, with spectinomycin-resistant mutants of either the salmonella gallinarum 9r vaccine or a rough, aroa insertion mutant of a s. enteritidis phage type 4 strain. the 9r strain was given intramuscularly, the rough, aroa mutant both intramuscularly and orally. two weeks after the second vaccination the immunized and a control group of unimmunized birds were challenged with a nalidixic acid-resistant mutant of a fully vir ...199118680065
persistence of salmonella enteritidis in young chickens.one- and 7-day-old specific pathogen-free chickens were artificially infected with a field isolate of salmonella enteritidis, phage type 13a. at intervals up to 42 days of age, birds were killed and liver, lung, spleen, brain, yolk sac, testis or ovary, duodenum, jejunum, ileum, caecum and colon collected for culture. in chickens infected at one day of age and killed 1, 4 and 7 days pi all tissues were infected. thereafter most non-intestinal tissues (74%) and intestinal tissues (92%) were posit ...199118680039
experimental infection of egg-laying hens with salmonella enteritidis phage type 4.chickens were inoculated intravenously with 10(5) or 10(6) organisms. heavy infection of the ovaries occurred and some infection persisted in this organ for several weeks. most of the ovarian infections were confined to the interstitial tissues and not to the yolk contained in the large follicles. infections of the ovary did not result from contamination from infected air sacs. none of 810 eggs laid contained s. enteritidis. chickens infected orally gave similar results to those following intrav ...199118680027
experimental infection of chickens with salmonella enteritidis.following oral inoculation of newly hatched commercial broilers, four phage types of salmonella enteritidis showed considerable variation in their virulence. the percentage mortality varied from 96%, produced by a phage type 4 strain, to 20% (phage type 13a). when high mortality was observed, chickens that died showed a polyserositis with pericarditis similar to that observed in naturally occurring cases. strains of phage types 4, 6 and 8 were more virulent than a phage type 13a strain when inoc ...199118680007
differential adaptive response and survival of salmonella enterica serovar enteritidis planktonic and biofilm cells exposed to benzalkonium chloride.this study examined the adaptive response and survival of planktonic and biofilm phenotypes of salmonella enterica serovar enteritidis adapted to benzalkonium chloride (bc). planktonic cells and biofilms were continuously exposed to 1 microg ml(-1) of bc for 144 h. the proportion of bc-adapted biofilm cells able to survive a lethal bc treatment (30 microg ml(-1)) was significantly higher (4.6-fold) than that of bc-adapted planktonic cells. similarly, there were 18.3-fold more survivors among the ...200818663028
identification of the genetic determinants of salmonella enterica serotype typhimurium that may regulate the expression of the type 1 fimbriae in response to solid agar and static broth culture conditions.type 1 fimbriae are the most commonly found fimbrial appendages on the outer membrane of salmonella enterica serotype typhimurium. previous investigations indicate that static broth culture favours s. typhimurium to produce type 1 fimbriae, while non-fimbriate bacteria are obtained by growth on solid agar media. the phenotypic expression of type 1 fimbriae in s. typhimurium is the result of the interaction and cooperation of several genes in the fim gene cluster. other gene products that may als ...200818652702
myocarditis related to salmonella enteritidis infection.myocarditis associated with bacterial enteritis has only rarely been described in literature. the clinical manifestation of the disease is often oligosymptomatic, so the real incidence could be underestimated. a case of myocarditis in a 31-year-old male patient having salmonella enteritidis infection is reported. the clinical course and problems concerning the diagnosis are discussed. the possibility of myocardial infection should be considered in any patient with cardiac complaints during gastr ...200718651527
effect of blood spots in table egg albumen on salmonella growth.presence of blood spots in eggs has been correlated with a greater rate of salmonella enteritidis contamination. therefore, this study was conducted to determine whether salmonella inoculated into egg albumen with naturally occurring blood spots would survive or grow. in each of 3 trials, white shell table eggs with blood spots were collected from a commercial egg-processing plant after candling. in each trial, eggs were broken out, and approximately 4 ml of clear albumen (clear) and 4 ml of blo ...200818648063
validation of cooking methods using shell eggs inoculated with salmonella serotypes enteritidis and heidelberg.salmonella enterica serotype enteritidis has long been associated with eggs, and more recently, salmonella enterica serotype heidelberg has also become associated with eggs. this study was undertaken to determine whether salmonella enteritidis and salmonella heidelberg are effectively eliminated from eggs by various cooking methods. seven cooking methods were chosen--hard and soft cooked, scrambled, over easy, sunny-side up, poached, and free poached--and a pan insert and the free-flowing method ...200818648060
salmonella enteritidis-induced alteration of inflammatory cxcl chemokine messenger-rna expression and histologic changes in the ceca of infected chicks.to understand better the events in early avian host immune responses to salmonella enteritidis (se), we examined messenger-rna (mrna) expression for eight genes: cxcli1[k60], cxcli2 [il-8/caf], interferon (ifn)-gamma, interleukin (il)-1beta, il-6, il-12alpha, il-12beta, and gallinacin (gal)-2 in the ceca of young chicks 1 wk postinoculation with se. cecum tissue sections were stained and evaluated for the presence of macrophages, lymphocytes, heterophils, and apoptotic cells following se infecti ...200818646451
evaluation of salmonella enteritidis-immune lymphokines on host resistance to salmonella enterica ser. gallinarum infection in broiler chicks.the prophylactic treatment of neonatal broiler chicks with lymphokines derived from s. enteritidis-immurazed chickens (se-ilk) was evaluated for its effect on the birds' resistance to an experimental infection s. enterica ser. gallinarum (sg). on the day of hatch, chicks were injected intraperitoneally with either se-ilk, control non-immune lymphokines (nilk), or were left untreated. thirty minutes later, all chicks were orally gavaged with either 10(4) colony forming units (cfu) or 10(6) cfu sg ...199618645895
the antibacterial effects for salmonella enteritidis phage type 4 of different chemical disinfectants and cleaning agents tested under different conditions.a selection of commercially available disinfectants, sanitizers and water sanitizers based on iodophor, quaternary ammonium compounds (qacs) and phenolic compounds were tested for their activity against a phage type 4 strain of salmonella serotype enteritidis in the presence of a variety of organic materials. in general the phenolic preparations were the most effective followed by the qacs and the iodophors. they were all inactivated to different degrees by chick fluff, chicken faeces, feed and ...199618645890
a retrospective case-control study of risk factors associated with salmonella enterica subsp. enterica serovar enteritidis infections on dutch broiler breeder farms.the organized control of salmonella enterica subsp. enterica serovar enteritidis (se) in the dutch poultry sector aims to prevent vertical transmission by the top-down approach, but its effectiveness is now undermined by the increasing role of horizontal transmission, so that preventive hygiene on the poultry farm gains significant importance. in order to assess the actual level of preventive hygiene and to identify risk factors, an inquiry was held among a representative number of broiler breed ...199518645784
resistance of chicks and poults fed vermicompost to caecal colonization by salmonella.vermicompost (vc) was produced by feeding the earthworm eisenia foetida vegetable matter and fresh faeces from adult specific pathogen-free chickens. this was used as a competitive exclusion treatment for chicks and poults against challenge with salmonella spp. one-day-old chicks and poults housed in floor pens, were treated with vc sprinkled on feed on days 1 and 2. for studies with salmonella typhimurium, chicks were challenged on day 5 by either oral inoculation with 10(4) colony-forming unit ...199518645773
growth-phase regulation of lipopolysaccharide o-antigen chain length influences serum resistance in serovars of salmonella.the amount of lipopolysaccharide (lps) o antigen (oag) and its chain length distribution are important factors that protect bacteria from serum complement. salmonella enterica serovar typhi produces lps with long chain length distribution (l-oag) controlled by the wzz gene, whereas serovar typhimurium produces lps with two oag chain lengths: an l-oag controlled by wzz(st) and a very long (vl) oag determined by wzz(fepe). this study shows that serovar enteritidis also has a bimodal oag distributi ...200818628492
[patient with purulent pericarditis caused by salmonella enteritidis complicated by tamponade].we report a case of a 64-year-old male patient with a history of advanced chronic heart failure, who was admitted to our hospital with symptoms of decompensated right heart failure. chronic tamponade was diagnosed and conservative treatment was administered. due to the lack of improvement, surgical drainage of the pericardial sack was performed and salmonella enteritidis was cultured from the pericardial fluid. after a month the patient fully recovered and was discharged.200818626836
analysis of the foodnet case-control study of sporadic salmonella serotype enteritidis infections using persons infected with other salmonella serotypes as the comparison group.use of well persons as the comparison group for laboratory-confirmed cases of sporadic salmonellosis may introduce ascertainment bias into case-control studies. data from the 1996-1997 foodnet case-control study of laboratory-confirmed salmonella serogroups b and d infection were used to estimate the effect of specific behaviours and foods on infection with salmonella serotype enteritidis (se). persons with laboratory-confirmed salmonella of other serotypes acted as the comparison group. the ana ...200918611288
clinical presentation of extraintestinal infections caused by non-typhoid salmonella serotypes among patients at the university hospital in cracow during an 7-year period.the most characteristic finding in non-typhoid salmonella (nts) infection is acute food related outbreaks of gastroenteritis, which is usually benign and self-limiting. however, more serious extraintestinal findings, such as bacteraemia and focal infections localized to any organ may appear. the objective of this paper is to describe the most important characteristic of the extraintestinal infections due to nts serotypes observed in university hospital, in cracow between january 2000 and decembe ...200818610655
importance of the major fli c antigenic site of salmonella enteritidis as a subunit vaccine antigen.our previous study indicated that the antibody against the major antigenic site of se fli c (g.m. region) is characteristically produced after the application of se bacterin, however, the antibody is not produced in chickens after se infection. in the present study, we determined histologically if the major antigenic site could be a candidate antigen for se subunit vaccine. when layermune se, a commercial se bacterin, was injected subcutaneously into the shoulder region as a positive control, th ...200818603341
il-17a is produced by th17, gammadelta t cells and other cd4- lymphocytes during infection with salmonella enterica serovar enteritidis and has a mild effect in bacterial clearance.t(h)17 cells represent a new pro-inflammatory t(h) cell lineage distinct from t(h)1 and t(h)2 cells. t(h)17 cells have been shown to be involved in extracellular bacterial infection but their role in intracellular infection remains unclear. we found antigen-specific il-17a production during a systemic infection of mice with the facultative intracellular bacterium salmonella enterica serovar enteritidis (s. enteritidis) and examined the function and cellular source of il-17a during the adaptive i ...200818599501
comparative genome analysis of salmonella enteritidis pt4 and salmonella gallinarum 287/91 provides insights into evolutionary and host adaptation pathways.we have determined the complete genome sequences of a host-promiscuous salmonella enterica serovar enteritidis pt4 isolate p125109 and a chicken-restricted salmonella enterica serovar gallinarum isolate 287/91. genome comparisons between these and other salmonella isolates indicate that s. gallinarum 287/91 is a recently evolved descendent of s. enteritidis. significantly, the genome of s. gallinarum has undergone extensive degradation through deletion and pseudogene formation. comparison of the ...200818583645
coated fatty acids alter virulence properties of salmonella typhimurium and decrease intestinal colonization of pigs.salmonella typhimurium infections in pigs are a major source of human foodborne salmonellosis. to reduce the number of infected pigs, acidification of feed or drinking water is a common practice. the aim of the present study was to determine whether some frequently used short- (scfa) and medium-chain fatty acids (mcfa) are able to alter virulence gene expression and to decrease salmonella typhimurium colonization and shedding in pigs using well established and controlled in vitro and in vivo ass ...200818583068
f-18 fdg pet/ct in the management of infected abdominal aortic aneurysm due to salmonella.mycotic aneurysm is a rare and life-threatening disorder. computed tomography (ct) is considered to be the best diagnostic imaging modality that can detect an abdominal aortic aneurysm and changes in the surrounding structures. more recently, f-18 fluorodeoxyglucose (fdg) pet would seem to hold promise for the diagnosis of focal infection and during the follow-up after antibiotic treatment. we present a case of an infected abdominal aortic aneurysm due to salmonella enteritidis. in this case, a ...200818580240
prediction of an organism's inactivation patterns from three single survival ratios determined at the end of three non-isothermal heat treatments.traditionally, an organism's heat resistance parameters have been determined from a set of experimental isothermal survival data. sometimes, however, even approximating an isothermal profile, and/or obtaining counts at sufficiently short time intervals, is extremely difficult for technical and logistic reasons. the problem would be avoided if the survival parameters could be calculated from the final survival ratios determined at the end of non-isothermal heat treatments with known temperature p ...200818579249
the influence of a fructooligosaccharide prebiotic combined with alfalfa molt diets on the gastrointestinal tract fermentation, salmonella enteritidis infection, and intestinal shedding in laying hens.molting is a natural process, which birds undergo to rejuvenate their reproductive organs. the us poultry egg production industry has used feed withdrawal to effectively induce molt; however, susceptibility of salmonella enteritidis has encouraged the development of alternative methods. previous research conducted in our laboratory showed that alfalfa is effective at molt induction and provides equivalent postmolt production numbers and quality when compared with feed withdrawal. in the attempt ...200818577603
foodborne salmonella ecology in the avian gastrointestinal tract.foodborne salmonella continues to be a major cause of salmonellosis with salmonella enteritidis and s. typhimurium considered to be responsible for most of the infections. investigation of outbreaks and sporadic cases has indicated that food vehicles such as poultry and poultry by-products including raw and uncooked eggs are among the most common sources of salmonella infections. the dissemination and infection of the avian intestinal tract remain somewhat unclear. in vitro incubation of salmone ...200918577459
salmonella enteritidis meningitis in a first time diagnosed aids patient: case report.abstract: we describe a patient with salmonella enteritidis meningitis and unknown hiv infection.setting: a 14-bed adult intensive care unit in a tertiary hospital.the patient was brought to the emergency department with fever, nuchal rigidity and confusion. a first cerebrospinal fluid examination was non diagnostic. after a short period of improvement the patient developed septic shock. a second cerebrospinal fluid specimen was purulent. both specimens yielded salmonella enteritidis and a blood ...200818577242
edible coatings for enhancing microbial safety and extending shelf life of hard-boiled eggs.hard-boiled eggs were coated with chitosan-lysozyme (cl), whey protein isolate (wpi), or bake sheen (bs), inoculated with listeria monocytogenes or salmonella enterica ser. enteritidis at 10(4) cfu/g, and stored for 4 wk at 10 degrees c. microbial populations were enumerated weekly. two nonchallenge studies were also conducted with hard-boiled eggs: coated shell-on eggs packaged in plastic containers or peeled eggs coated and vacuum-packaged. total plate counts (tpc), coliforms, yeasts, molds, w ...200818577005
focal salmonella enteritidis infection in a patient with hiv infection and other multiple causes of immunodeficiency.an hiv-infected man receiving antiretroviral therapy-who also had lupus-like vasculitis and membranous glomerulonephritis (treated with prednisolone and azathioprine), beta-thalassaemia minor trait and post-radiotherapy functional asplenia (mimicking sickle cell disease-induced hyposplenism)-developed focal soft issue and bone infection caused by salmonella enteritidis at the site of previous mycobacterial infection.200818574127
class 1 integrons in multidrug-resistant non-typhoidal salmonella enterica isolated in spain between 2002 and 2004.in this study, 119 multidrug-resistant isolates of non-typhoidal salmonella enterica serovars collected in spain (2002-2004) were screened for integrons. among the isolates, 73.1% contained class 1 integrons, however classes 2 and 3 were not detected. integrons containing gene cassettes were found in s. enteritidis (16/32), s. typhimurium biphasic (18/32) and monophasic [4,5,12:i:-] (11/19), s. virchow (17/18) and s. brandenburg (8/8), but not in s. hadar (0/10). ten complete and four incomplete ...200818571383
molecular diagnosis of salmonella species in captive psittacine birds.cloacal swabs were collected from 280 captive psittacine birds belonging to 13 species. samples of dna were tested by pcr using a pair of primers that amplify a 284 base pair fragment of the salmonella genus inva gene, and the pcr-positive samples were tested by standard microbiological techniques. thirteen per cent of the samples were positive by pcr, but negative by microbiological techniques. the infection rates were significantly different among the 13 species, the most commonly infected bei ...200818567929
mutations influencing expression of the salmonella enterica serovar enteritidis pathogenicity island i key regulator hila.invasion in gut epithelial cells, mediated by genes of the salmonella pathogenicity island i, is a crucial step in the pathogenesis of salmonella enterica serovar enteritidis infections. the most important regulator of the invasive process is the hila gene. in this study, a transposon bank approach was used to identify dna sequences affecting expression of hila. mutants with decreased hila expression carried mutations in known virulence gene regulators (fliz, hild, sira), genes encoding ion tran ...200818563621
trehalose: a biophysics approach to modulate the inflammatory response during endotoxic shock.we evaluated the effects of trehalose against endotoxic shock, a condition in which the loss of bio-membrane integrity plays a pivotal role. in addition we performed a biophysics experiment by quasi elastic neutron scattering (qens) study, to investigate whether the membrane stability effect of trehalose might be correlated with its high capability to switch-off the water diffusive dynamics and, hence, the kinetic mechanisms of interaction. endotoxic shock was induced in male rats by a single in ...200818555988
[chronic diarrhea. main symptoms: on frequent, on fluid stool and diarrhea--lasting > 3 weeks]. 200818548804
phage types and pulsed-field gel electrophoresis patterns of salmonella enterica serovar enteritidis isolated from humans and chickens.we analyzed 66 salmonella enteritidis isolates in 2002. thirty isolates were obtained from human patients with diarrhea, and 36 were obtained from chickens. a total of ten phage types (pt) were identified in the human and chicken isolates. pt1 and pt21 were the predominant pts in both the human (20% and 13%) and chicken (17% and 47%) isolates. twelve pulsotypes were generated by pfge and divided into two major groups. most of the pfge types were categorized into cluster group 1. eighteen chicken ...200818545971
in vitro evaluation of antibacterial, antioxidant, cytotoxic and apoptotic activities of the tubers infusion and extracts of cyperus rotundus.the in vitro antibacterial, antioxidant, cytotoxic and apoptotic activities from tubers extracts of cyperus rotundus (cyperaceae) were investigated. antibacterial activity of different extracts was evaluated against five bacterial reference strains. a marked inhibitory effect was observed against salmonella enteritidis, staphylococcus aureus and enterococcus faecalis with total oligomers flavonoids (tofs) and ethyl acetate extracts. in addition to their antibacterial activity, the same extracts ...200818538563
salmonella enteritidis bacteremia in at win pair. 200818536899
food-borne outbreak caused by salmonella enterica serovar enteritidis in bari, italy.this paper presents data from an epidemiologic investigation of an outbreak of gastroenteritis that occurred in bari, southern italy, in june 2005. the source of the outbreak was traced to consumption of rice "arancini" (a typical italian food prepared with rice, mozzarella cheese, eggs and bread crumbs), prepared in a traditional bakery in town. salmonella enteritidis strains were isolated from affected patients, bakery personnel and food items. isolates were analysed by using traditional micro ...200618536762
symptomatic acute reactive arthritis after an outbreak of salmonella.in 2005, 592 individuals in ontario developed acute gastroenteritis, predominantly after consuming bean sprouts contaminated with salmonella enteritidis. salmonella is a known trigger of reactive arthritis (rea). we describe the population affected by the salmonella outbreak in terms of clinical presentation of self-reported arthritic symptoms and hla-b27 genotyping.200818528961
purulent pericarditis with salmonella enteritidis in a patient with cd4/cd8 depression.a 65-year-old man was admitted for high-grade fever with a shaking chill and general fatigue. chest x-ray showed cardiomegaly, and echocardiography revealed a large amount of pericardial effusion. emergency pericardiocentesis was performed, and salmonella enteritidis was found in pericardial fluids. we diagnosed purulent pericarditis with s. enteritidis, and administered antibiotics. while high-grade fever resolved 10 days after beginning of treatment, effusive-constrictive pericarditis (ecp) wi ...200818522796
reduction of salmonella enteritidis population sizes on almond kernels with infrared heat.catalytic infrared (ir) heating was investigated to determine its effect on salmonella enterica serovar enteritidis population sizes on raw almond kernels. using a double-sided catalytic ir heating system, a radiation intensity of 5,458 w/m2 caused a fast temperature increase at the kernel surface and minimal temperature differences between the top and bottom kernel surfaces. exposure of dry kernels to ir heat for 30, 35 and 45 s resulted in maximum kernel surface temperatures of 90, 102, and 11 ...200818522020
multi-locus variable-number tandem repeat analysis for outbreak studies of salmonella enterica serotype enteritidis.salmonella enterica subsp. enterica serotype enteritidis is known as an important and pathogenic clonal group which continues to cause worldwide sporadic cases and outbreaks in humans. here a new multiple-locus variable-number tandem repeat analysis (mlva) method is reported for highly-discriminative subtyping of salmonella enteritidis. emphasis was given on the most predominant phage types pt4 and pt8. the method comprises multiplex pcr specifically amplifying repeated sequences from nine diffe ...200818513386
[survival of salmonella in spices and growth in cooked food].contamination of spices with pathogens has been reported worldwide, and salmonella might result in foodborne infections. in this study, we investigated the survival of salmonella in black pepper and red pepper, and the growth of the surviving salmonella in cooked food. salmonella enteritidis, salmonella weltevreden and salmonella senftenberg were inoculated into spices, and their survival during storage was examined. in black pepper, s. enteritidis was no longer viable after storage for 28 days, ...200818503241
[plasmid profile analysis in identification of epidemic strains of salmonella enterica serovar enteritidis].as illness caused by sallmonella enterica serovar enteritidis (s. enteritidis) occurs not only as sporadic cases but as outbreaks, to reveal the source and routes of spreading of infection it is necessary to identify epidemic strain by the use of some typing methods. to determine whether plasmid profile analysis, as genotyping method, could be applied for the investigation of epidemic strains, isolates of s. enteritidis, recovered from patient's stools and food associated with outbreaks and thos ...200818499952
influence of exogenous melatonin administration on salmonella enteritidis colonization in molted layers.two studies were conducted to evaluate the effects of melatonin on salmonella enteritidis infection in experimentally challenged laying hens subjected to a forced molt. leghorn hens (>50 wk of age) were randomly assigned to rooms, acclimated to a 16l:8d regimen, and provided ad libitum access to a nonmedicated mash layer diet and water. birds in one room were molted (8l:16d; complete feed withdrawal), whereas birds in the second room served as nonmolted controls (cont). within each room, birds w ...200818492995
differentiation of salmonella typhimurium from salmonella enteritidis and other salmonella serotypes using random amplified polymorphic dna analysis.salmonella enterica ssp. enterica serovar typhimurium and salmonella enterica ssp. enterica serovar enteritidis are the major dominating serotypes of salmonella in poultry and poultry products. infection by salmonella typhimurium is an important cause of morbidity and mortality in poultry. rapid differentiation of salmonella typhimurium from other salmonella serotypes including salmonella enteritidis can be very crutial for public health and for epidemiologists and for the poultry industry. ten ...200818492993
administration of salmonella enteritidis-immune lymphokine to day-old turkeys by subcutaneous, oral, and nasal routes: a comparison of effects on salmonella enteritidis liver invasion, peripheral blood heterophilia and heterophil activation.previous experiments have shown that the administration by intraperitoncal (i.p.) injection of salmonella enteritidis-immune iymphokines (lk) derived from chicken splenic t cells protect both chicks and turkey poults from salmonella enteritidis (se) organ invasion. this protection was mediated by the avian heterophil. the present experiments evaluated the ability of se-immune iymphokines derived from a virally transformed chicken t cell line (vilk) in protecting day-old turkeys against se liver ...199818484275
evaluation of combined antibiotic and competitive exclusion treatment in broiler breeder flocks infected with salmonella enterica serovar enteritidis.strategic medication in breeding flocks of domestic fowl (gallus gallus), using antimicrobial treatment followed by competitive exclusion, was evaluated in 13 trials between february and september 1993. in each trial, the flock had been confirmed as naturally infected with salmonella enterica serovar enteritidis and the effect of treatment was determined on salmonella isolation from tissues. of 11 trials where enrofloxacin was used, a long-term reduction of salmonella was observed in two and a s ...199718483892
chest wall mass caused by salmonella enteritidis - a pitfall of pet imaging interpretation. 200818481248
review of induced molting by feed removal and contamination of eggs with salmonella enterica serovar enteritidis.as laying hens age, egg production and quality decreases. egg producers can impose an induced molt on older hens that results in increased egg productivity and decreased hen mortality compared with non-molted hens of the same age. this review discusses the effect of induced molting by feed removal on immune parameters, salmonella enterica serovar enteritidis (se) invasion and subsequent production of se-contaminated eggs. experimental oral infections with se show molted hens are more susceptible ...200818479846
first-trimester septic abortion due to salmonella enteritidis oranienburg.septic abortion caused by transplacental salmonella infection is extremely rare; there are no reported cases of serotype oranienburg as an etiology.199518475401
water pressure effectively reduces salmonella enterica serovar enteritidis on the surface of raw almonds.the effects of continuous high hydrostatic water pressure treatments (414 and 483 mpa with a holding time of 6 min) on the viability of two strains of salmonella enterica serovar enteritidis (pt 9c and pt 30) inoculated onto raw almonds were evaluated at 25 degrees c. the concentrations of both salmonella enteritidis isolates were reduced to undetectable levels when the almonds were directly suspended in water, pressurized at 414 mpa and 25 degrees c for 6 min, and then dried at 115 degrees c fo ...200818468041
antimicrobial activity of some new thioureides derived from 2-(4-chlorophenoxymethyl)benzoic acid.we report here the characterisation of eight newly synthesized thioureides of 2-(4-chlorophenoxymethyl)-benzoic acid and the evaluation of the in vitro antimicrobial activity of the new compounds against gram-positive [listeria monocytogenes,staphylococcus aureus, bacillus subtilis], gram-negative [psedomonas aeruginosa,escherichia coli, salmonella enteritidis], as well as candida spp., using both reference and clinical multidrug resistant strains to establish the minimal inhibitory concentratio ...200818463566
effect of a selected lactobacillus spp.-based probiotic on salmonella enterica serovar enteritidis-infected broiler chicks.the effect of a lactobacillus spp.-based probiotic (fm-b11) on salmonella enterica serovar enteritidis (se) recovery was evaluated in liquid (expt. 1) and lyophilized (expt. 2) forms in two separate experiments with two trials each. for each trial, 80 broiler chicks were randomly allocated into two treatments: control and probiotic culture. all chicks were challenged with se (approximately 10(4) colony-forming units [cfu]) upon arrival at our laboratory. in both experiments, probiotic culture wa ...200818459312
serovar-specific real-time quantitative detection of salmonella enteritidis in the gastrointestinal tract of ducks after oral challenge.the objective of this study was to identify and understand the regular distribution pattern and primary penetration site for salmonella enteritidis (se) in the gastrointestinal tract of ducks. an assay based on the serovar-specific dna sequence of se from genbank, a serovar-specific real-time, fluorescence-based quantitative polymerase chain reaction, was developed for the detection of se. we used this assay to detect genomic dna of se in the blood and gastrointestinal tract, including duodenum, ...200818459302
crop immune response post-salmonella enteritidis challenge in eight commercial egg-layer strains and specific-pathogen-free white leghorn chickens.the crop immune response against salmonella enteritidis (se) challenge in eight commercial egg-layer strains (five white-egg layer and three brown-egg layer) and specific-pathogen-free (spf) white leghorn (wl) hens was investigated. pre- and post-se challenge mucosal immune responses within the crops were evaluated. commercial layers and spf wl hens were orally challenged with 10(8) cfu/ml se pt13a and se nalr pt13, respectively. crop lavage samples were collected at weekly intervals from day 0 ...200818459301
bacteriophage treatment reduces salmonella colonization of infected chickens.three different lyric bacteriophages (bps) were isolated from the sewage system of commercial chicken flocks and used to reduce salmonella enteritidis (se) colonization from experimental chickens. ten-day-old chickens were challenged with 9.6 x 10(5) colony-forming units (cfu)/ml of a se strain and treated by coarse spray or drinking water with a cocktail of the three phages at a multiplicity of infection (mo1) of 10(3) plaque-forming units (pfu) 24 hr prior to se challenge. chickens were euthan ...200818459298
characterization of tem-, shv- and ampc-type beta-lactamases from cephalosporin-resistant enterobacteriaceae isolated from swine.extended-spectrum beta-lactamase (esbl) and ampc-producing enterobacteriaceae are an increasing problem in human medicine and an emerging problem in the veterinary field. our study, therefore, focused on assessing the prevalence of beta-lactamases isolated from swine. sixty-six salmonella enterica serovar typhimurium (s. typhimurium), 33 salmonella enterica serovar enteritidis (s. enteritidis), 26 klebsiella pneumonia (k. pneumoniae) and 130 escherichia coli (e. coli) pig isolates collected from ...200818455821
epidemics of invasive salmonella enterica serovar enteritidis and s. enterica serovar typhimurium infection associated with multidrug resistance among adults and children in malawi.nontyphoidal salmonellae (nts) have become the most common cause of bacteremia in tropical africa, particularly among susceptible children and hiv-infected adults.200818444810
increased production of the ether-lipid platelet-activating factor in intestinal epithelial cells infected by salmonella enteritidis.when exposed to enteric pathogens intestinal epithelial cells produce several cytokines and other proinflammatory mediators. to date there is no evidence that the ether-lipid platelet-activating factor (paf) is one of these mediators. our results revealed a significant increase in paf production by human colonic tissue 4 h after infection by enterohemorrhagic escherichia coli (ehec) or salmonella enteritidis. paf is produced in the gut by cells of the immune system in response to bacterial infec ...200818442488
trends in phage types and antimicrobial resistance of salmonella enterica serovar enteritidis isolated from animals in great britain from 1990 to 2005.surveillance data for salmonella enterica serovar enteritidis incidents and isolations from food animals in great britain from 1990 to 2005 were analysed to detect any trends and provide the basis for a comparison between phage types (pt) and antimicrobial sensitivity patterns in human beings and animals. during 2001 to 2005 there was a decrease in incidents involving most species except ducks. only the numbers of incidents involving pts 6, 6a, 9b and 14b (in ducks) and pts 6a and 13a (in mammal ...200818441349
inactivation of salmonella enterica serovar enteritidis in shell eggs by sequential application of heat and ozone.to assess the contribution of ozone to lethality of salmonella enterica serovar enteritidis in experimentally inoculated whole shell eggs that are sequentially treated with heat and gaseous ozone in pilot-scale equipment.200818422940
[technic study on multilocus sequence typing system of salmonella enteritidis].to characterize different salmonella enterica enteritidis isolates through multilocus sequence typing (mlst) system, a molecular epidemiologic analysis method was established.200818421863
reduction of salmonella enteritidis in shell eggs using directional microwave technology.microwaves have been shown to cause thermal as well as nonthermal destruction of pathogens such as salmonella enteritidis, which is commonly found in shell eggs. the objective of this study was to examine the use of new directional microwave technology to reduce salmonella enteritidis without causing any detrimental effects on quality in white and brown eggs. treatments were control and microwaved white and brown eggs. applying directional microwave technology resulted in a 2-log reduction of sa ...200818420992
detection of t lymphocytes in intestine of broiler chicks treated with lactobacillus spp. and challenged with salmonella enterica serovar enteritidis.the expression of immune response in the form of leukocytic infiltrate by cd3+, cd4+, and cd8+ cells in the epithelium and in the intestinal lamina propria of chicks was studied in the present work by means of immunohistochemical reaction. the chicks were treated with lactobacillus spp. or cecal microflora (cm) and experimentally challenged or not with salmonella enterica serovar enteritidis. the 320 birds utilized were divided into 4 groups containing 80 chicks each and submitted to treatments ...200818420983
prevention of spontaneous abortion in the cba x dba/2 mouse model by intravaginal tgf-beta and local recruitment of cd4+8+ foxp3+ cells.activation of latent transforming growth factor (tgf)-beta in seminal plasma has been suggested by robertson et al. to promote maternal tolerance to paternal antigens. a possible consequence reported by tremellen et al. is increased pregnancy rates in women undergoing ivf. a decreased spontaneous abortion rate has also been postulated. seminal plasma contains many factors besides tgf-beta, and a critical test of the hypothesis was required. the purpose of the present study was to directly test t ...200818410469
dietary conjugated linoleic acid improves antioxidant capacity in broiler chicks.1. the influence of dietary conjugated linoleic acid (cla) on the antioxidant status in the absence or presence of endotoxin exposure was studied with male broiler chicks. 2. in experiment 1, a total of 240 1-d-old broilers were allotted into 4 dietary groups (0, 2.5, 5.0 or 10.0 g pure cla/kg) to study the influence of cla on growth performance and antioxidant defence systems. the results showed that growth performance was not altered by 42 d of cla consumption. increased total superoxide dismu ...200818409096
purification of bacteriocin ls1 produced by human oral isolate lactobacillus salivarius bgho1.lactobacillus salivarius bgho1, a human oral isolate with antagonistic activity against growth of streptococcus mutans, streptococcus pneumoniae, staphylococcus aureus, enterococcus faecalis, micrococcus flavus, and salmonella enteritidis, probably produces more than one proteinaceous antimicrobial substance. the objective of this study was the purification of a bacteriocin, named ls1, produced by l. salivarius bgho1.200818402613
[prevalence of salmonella sp. in foods in tamaulipas, mexico, during 2005].to determine the prevalence of salmonella sp. in foods obtained from tamaulipas sanitary jurisdictions during 2005.200718402335
toll-like receptor gene expression in cecum and spleen of advanced intercross line chicks infected with salmonella enterica serovar enteritidis.understanding the changes in host gene expression that occur with bacterial infection will help to elucidate the basis of molecular genetic control of disease resistance. the effect of infecting chicks with salmonella enterica serovar enteritidis on the rna expression level of toll-like receptor (tlr) genes, and the correlation between tlr rna expression level and bacterial burden in the cecum and spleen of young birds was studied. chicks from two advanced intercross lines were either infected o ...200818394716
biodegradable polylactic acid polymer with nisin for use in antimicrobial food packaging.biodegradable polylactic acid (pla) polymer was evaluated for its application as a material for antimicrobial food packaging. pla films were incorporated with nisin to for control of foodborne pathogens. antimicrobial activity of pla/nisin films against listeria monocytogenes, escherichia coli o157:h7, and salmonella enteritidis were evaluated in culture media and liquid foods (orange juice and liquid egg white). scanned electron micrograph and confocal laser microscopy revealed that nisin parti ...200818387115
effect of hydrostatic pressure pulsing on the inactivation of salmonella enteritidis in liquid whole egg.eggs and egg-containing foods contaminated with bacterial human pathogens have been implicated in numerous foodborne outbreaks leading to costly recalls. research was undertaken to investigate the use of high pressure-pulse treatment to inactivate salmonella enteritidis inoculated in liquid egg. liquid egg was inoculated with salmonella enteritidis (8.0 log colony-forming units [cfu]/ml) and exposed to hydrostatic pressures (300-400 mpa) and pressure (350 mpa) pulsing at 25 degrees c, 40 degrees ...200818377201
Refresh
firstprev
...11121314151617181920...
nextlast
Displaying items 1701 - 1800 of 6195