Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| trichomoniasis among women with vaginal discharge in jeddah city, saudi arabia. | during the year of 2003, vaginal discharge specimens were collected from 1767 women aged (15-50) in 6 cardinal hospitals in jeddah city of saudi arabia. the samples were examined for trichomonas vaginalis, a prevalence rate of 12 (0.7%) were positive. demographic characters did not show significant relation to the infection rate. all accompanied symptoms were insignificant (p>0.05) except the vaginal consistency which was significant (p<0.05). the studied risk factors were insignificant to the t ... | 2005 | 16333911 |
| characterization of specific trichomonas vaginalis target antigens from different isolates by immunoblotting against hyperimmune rabbit serum. | the sds-page and immunoblot methods were used to identify trichomonas vaginalis specific target antigen(s). ten t. vaginalis isolates, cultured on tym media, were analyzed by sodium dodecyl sulphate poly acrylamide gel electrophoresis (sds-page), revealed 34 distinct bands. immunoblotting against a hyperimmune rabbit serum, showed 20 reactive bands ranging from 14-205 kda. there was isolate to isolate variability among 8 isolates, while 2 isolates (6,7) showed a similar antigenic patterns. imuno ... | 2005 | 16333897 |
| aetiology of sexually transmitted infections and response to syndromic treatment in southwest uganda. | to determine the aetiology of genital ulcers and discharges in rural south western uganda and to assess response to syndromic treatment. | 2005 | 16326853 |
| laboratory diagnosis of sexually transmitted infections in estonia in 2001-2002: shortcomings with impact on diagnostic quality and surveillance. | the objectives of this study were to comprehensively characterize the range, content, and performance of sexually transmitted infection (sti) testing services in estonia during the period 2001 to 2002 and to determine if the observed diagnostic laboratory practices and methods adhered to international evidence-based recommendations. | 2005 | 16314773 |
| synthesis and antiprotozoal activity of some new synthetic substituted quinoxalines. | a series of 29 new quinoxalines was synthesized and evaluated in vitro against several parasites (leishmania donovani, trypanosoma brucei brucei, and trichomonas vaginalis). several of them displayed interesting activities, and particularly four quinoxaline amides showed in vitro antileishmanial properties (ic50 less than 20 microm). | 2006 | 16309903 |
| alternative pathway of metronidazole activation in trichomonas vaginalis hydrogenosomes. | metronidazole and related 5-nitroimidazoles are the only available drugs in the treatment of human urogenital trichomoniasis caused by the protozoan parasite trichomonas vaginalis. the drugs are activated to cytotoxic anion radicals by their reduction within the hydrogenosomes. it has been established that electrons required for metronidazole activation are released from pyruvate by the activity of pyruvate:ferredoxin oxidoreductase and transferred to the drug by a low-redox-potential carrier, f ... | 2005 | 16304169 |
| the 5s ribosomal rna gene from the early diverging protozoa trichomonas vaginalis. | 2006 | 16300840 | |
| prevalence, incidence, natural history, and response to treatment of trichomonas vaginalis infection among adolescent women. | trichomonas vaginalis infection is a sexually transmitted infection (sti) linked with reproductive health complications. however, few data exist concerning the epidemiologic profile of this pathogen in adolescent women, a group at high risk for other stis. | 2005 | 16288365 |
| trichomonas vaginalis lipophosphoglycan mutants have reduced adherence and cytotoxicity to human ectocervical cells. | the extracellular human pathogen trichomonas vaginalis is covered by a dense glycocalyx thought to play a role in host-parasite interactions. the main component of the glycocalyx is lipophosphoglycan (lpg), a polysaccharide anchored in the plasma membrane by inositol phosphoceramide. to study the role of lpg in trichomonads, we produced t. vaginalis lpg mutants by chemical mutagenesis and lectin selection and characterized them using morphological, biochemical, and functional assays. two indepen ... | 2005 | 16278462 |
| microbial isolates and hiv infection in couples attending fertility clinics in sagamu, nigeria. | this observational study was designed to determine the prevalence of hiv infection and the microbial isolates from the genital tracts of couples attending the fertility clinics of olabisi onabanjo university teaching hospital, sagamu, ogun state, nigeria between january 2001 and december 2002. a total of 110 women and 49 of their male partners were recruited into the study. the majority of the patients were between 25 and 34 years (women: 71.8%; partners: 65.3%), though the men were significantl ... | 2005 | 16263544 |
| synthesis and antiprotozoal activity of some 2-(trifluoromethyl)-1h-benzimidazole bioisosteres. | a series of 2-(trifluoromethyl)-1h-benzimidazole derivatives with various 5- and 6-position bioisosteric substituents (-cl, -f, -cf3, -cn), namely 1-7, were prepared using a short synthetic route. each analogue was tested in vitro against the protozoa giardia intestinalis and trichomonas vaginalis in comparison with albendazole and metronidazole. several analogues had ic50 values < 1 microm against both species, which make them significantly more potent than either standard. compound 4 [2,5(6)-b ... | 2006 | 16260067 |
| kinetics of immunoglobulin g, m, a and igg subclass responses in experimental intravaginal trichomoniasis: prominence of igg1 response. | trichomoniasis, the most common nonviral sexually transmitted disease, is caused by infection with the protist trichomonas vaginalis. the clinical spectrum varies from an asymptomatic state to mild, moderate or severe symptoms. however, the exact factors leading to varied symptomatology have not been well elucidated. the mouse is a useful experimental model for intravaginal trichomoniasis, for understanding the role of local immune responses in the pathogenesis and varied symptomatology of this ... | 2005 | 16255745 |
| diagnosis of trichomonas vaginalis infection: the sensitivities and specificities of microscopy, culture and pcr assay. | the aim of this study was to compare wet mount-, giemsa stain-, acridine orange fluorescent stain-, cultivation- and polymerase chain reaction (pcr)-based approaches to establish which method or combination of methods was most effective in the laboratory diagnosis of trichomoniasis. | 2006 | 16249051 |
| trichomonas vaginalis: identification of a triacylglycerol acylhydrolase. | this work describes the identification of a triacylglycerol lipase named tvlip directly onto blood-lb-agar plates by hemolytic screening of a trichomonas vaginalis cdna expression library. sharing significant similarity in the primary sequence with other lipases, the theoretical 3d structure of the tvlip was resolved. the structure reveals the predictive conserved characteristics of other lipases from ec3.1.1.3 group, although presenting one amino acid change in the catalytic triad ser-his-asp. ... | 2005 | 16236283 |
| sulfur-containing amino acid metabolism in parasitic protozoa. | sulfur-containing amino acids play indispensable roles in a wide variety of biological activities including protein synthesis, methylation, and biosynthesis of polyamines and glutathione. biosynthesis and catabolism of these amino acids need to be carefully regulated to achieve the requirement of the above-mentioned activities and also to eliminate toxicity attributable to the amino acids. genome-wide analyses of enzymes involved in the metabolic pathways of sulfur-containing amino acids, includ ... | 2005 | 16230102 |
| the clinical outcome of 137 rape victims in hong kong. | from 1 august 2001 to 31 july 2004, 137 patients were referred from the association concerning sexual violence against women to the accident and emergency department at the kwong wah hospital for alleged rape. approximately half of the patients presented within 3 days of the alleged assault. fifty-one patients were prescribed emergency contraception: one patient remained pregnant despite treatment and was referred with a further six patients to the gynaecology department for termination of pregn ... | 2005 | 16219959 |
| trichomonas vaginalis perturbs the junctional complex in epithelial cells. | trichomonas vaginalis, a protist parasite of the urogenital tract in humans, is the causative agent of trichomonosis, which in recent years have been associated with the cervical cancer development. in the present study we analyzed the modifications at the junctional complex level of caco-2 cells after interaction with two isolates of t. vaginalis and the influence of the iron concentration present in the parasite's culture medium on the interaction effects. our results show that t. vaginalis ad ... | 2005 | 16212877 |
| [comparative assessment of the diagnostic value of the laboratory diagnostic methods for trichomoniasis]. | the authors compared the sensitivity and specificity of currently available methods for laboratory diagnosis of trichomoniasis, by examining 971 persons. the highest frequency of t. vaginalis was detected by studies of a stained smear (37.4%), culture tests (19.0%); polymerase chain reaction (pcr) (17.1%), immunofluorescence tests (12.7%), wet smear test (2.7%). enzyme immunoassay yielded positive results in 36.2% of cases. the use of pcr and culture tests frequently provided similar results (92 ... | 2005 | 16212090 |
| the prevalence of trichomoniasis in young adults in the united states. | the prevalence of trichomoniasis in the general population of the united states is unknown. this study provides the first population-based prevalence estimates of trichomoniasis among young adults in the united states. | 2005 | 16205299 |
| detection of bacterial vaginosis, trichomonas vaginalis infection, and vaginal candida infection: a comparative study of methods of extracting exudates, with and without a speculum, during pregnancy. | to compare the collection of vaginal exudates with and without the use of a speculum to diagnose vaginal infections. | 2005 | 16205190 |
| intranasal immunisation with a 62 kda proteinase combined with cholera toxin or cpg adjuvant protects against trichomonas vaginalis genital tract infections in mice. | trichomonosis, caused by the protozoan parasite trichomonas vaginalis, is one of the most frequent sexually transmitted diseases and is widely spread in all continents. trichomonas vaginalis as well as other protozoan organisms have high levels of proteolitic activity mainly of the cysteine-proteinase type. this activity is necessary for recognition and adhesion of the parasite to the superficial epithelial cells of the host. in the present study, we show that intranasal immunisation with a 62 k ... | 2005 | 16202417 |
| the incidence of sexually transmitted infections among frequently screened sex workers in a decriminalised and regulated system in melbourne. | to determine the incidence of sexually transmitted infections (sti) among decriminalised and regulated sex workers in victoria. | 2005 | 16199747 |
| adherence to human vaginal epithelial cells signals for increased expression of trichomonas vaginalis genes. | host parasitism by trichomonas vaginalis is complex, and the adhesion to vaginal epithelial cells (vecs) by trichomonads is preparatory to colonization of the vagina. since we showed increased synthesis of adhesins after contact with vecs (a. f. garcia, et al., mol. microbiol. 47:1207-1224, 2003) and more recently demonstrated up-regulated gene expression in vecs after parasite attachment (a. s. kucknoor, et al., cell. microbiol. 7:887-897, 2005), we hypothesized that enhanced expression of adhe ... | 2005 | 16177319 |
| qualitatively distinct patterns of cytokines are released by human dendritic cells in response to different pathogens. | dendritic cells produce cytokines that regulate the class of the adaptive immune response. microbial recognition is mediated, at least in part, by pattern recognition receptors such as toll-like receptors, which influence dendritic cell maturation. in humans it is not yet clear how intact pathogens modulate the developing immune response. to address the effects of intact pathogens on the maturation and effector functions of human dendritic cells, we investigated their responses to a number of mi ... | 2005 | 16162273 |
| endometrial microbial colonization and plasma cell endometritis after spontaneous or indicated preterm versus term delivery. | this study was undertaken to determine whether endometrial microbial colonization or plasma cell endometritis is increased after spontaneous versus indicated preterm delivery or a spontaneous term delivery. | 2005 | 16150268 |
| real-time pcr improves detection of trichomonas vaginalis infection compared with culture using self-collected vaginal swabs. | to compare a real-time polymerase chain reaction (pcr) assay with broth culture for the detection of trichomonas vaginalis using self-collected vaginal swabs. | 2005 | 16126499 |
| risk factors for bacterial vaginosis among bar and hotel workers in northern tanzania. | to determine risk factors for bacterial vaginosis (bv) among women working in the bars and hotels in moshi, northern tanzania. | 2005 | 16122097 |
| association of chlamydia trachomatis with persistence of high-risk types of human papillomavirus in a cohort of female adolescents. | human papillomavirus (hpv) infection is a necessary but not sufficient cause of cervical cancer. while chlamydia infection has been associated with cervical cancer, the meaning of this association remains unclear. the authors' objective was to investigate this association by evaluating whether concurrent genital tract infections are associated with hpv persistence, a precursor to cervical cancer. interview data and biologic samples for hpv, chlamydia trachomatis, neisseria gonorrhoeae, trichomon ... | 2005 | 16120706 |
| "shotgun" versus sequential testing. cost-effectiveness of diagnostic strategies for vaginitis. | although vaginitis is a common outpatient problem, only 60% of patients can be diagnosed at the initial office visit of a primary care provider using the office procedures of ph testing, whiff tests, normal saline, and potassium hydroxide preps. | 2005 | 16117745 |
| an ancient spliceosomal intron in the ribosomal protein l7a gene (rpl7a) of giardia lamblia. | only one spliceosomal-type intron has previously been identified in the unicellular eukaryotic parasite, giardia lamblia (a diplomonad). this intron is only 35 nucleotides in length and is unusual in possessing a non-canonical 5' intron boundary sequence, ct, instead of gt. | 2005 | 16109161 |
| sti/hiv co-infections in uch, ibadan, nigeria. | sexually transmitted infections (stis) are poorly recognised and inadequately treated in nigeria in spite of the fact that it constitutes a major risk for hiv transmission. this study was carried out to ascertain sti/hiv co-infection rate and to obtain relevant socio-demographic and reproductive health data associated with stis. this information is urgently needed for designing sti/hiv control strategies. all consenting patients with history suggestive of sti, who attended sti clinic at the univ ... | 2005 | 16104654 |
| identification of a very large rab gtpase family in the parasitic protozoan trichomonas vaginalis. | rab proteins are pivotal components of the membrane trafficking machinery in all eukaryotes. distinct rab proteins locate to specific endomembrane compartments and genomic studies suggest that rab gene diversity correlates with endomembrane system complexity; for example unicellular organisms generally possess 5-20 rab family members and the size of the repertoire increases to 25-60 in multicellular systems. here we report 65 open reading frames from the unicellular protozoan trichomonas vaginal ... | 2005 | 16099517 |
| [new alkylated derivates and dissociation constans of 3-hydroxy-1,2-benzisoxazole]. | in the reactions of 3-hydroxy-1,2-benzisoxazole (bio) and its 5-cl and 6-cl derivatives with ethyl bromoacetate were obtained the mixtures of o- and n- substituted esters but 7-no2-bio formed only o-ester. the products of n-aminomethylation of 5-no2-bio were active against trichomonas vaginalis. the structures of pure compounds have been confirmed by elementary analysis and ir spectrometry. determinations of dissociation constants (pka) of bio, 5-cl-bio, 6-cl-bio, 7-cl-bio, 5-f-bio, 5-no2-bio, 7 ... | 1992 | 16092412 |
| molecular characterization of egyptian trichomonas vaginalis clinical isolates by hsp70 restriction fragment length polymorphism. | molecular typing of 20 egyptian trichomonas vaginalis clinical isolates was performed using the restriction fragment length polymorphism (rflp) analysis employing a probe from the heat-inducible cytoplasmic hsp70 gene family hybridized with ecor l-digested genomic dna. in each of the isolates tested 5 to 6 distinct dna fragments ranging from 2.7 kb to 7.5 kb in size were detected. analysis of 13 isolates from symptomatic and 7 isolates from asymptomatic women revealed 6 distinct rflp pattern sub ... | 2005 | 16083077 |
| nonoxynol 9 as an additive therapy in metronidazole-resistant cases of vaginal trichomoniasis. | the present study was conducted on 30 female patients with metronidazole resistant vaginal trichomoniasis to investigate the efficacy of nonoxynol 9 (n-9), a non hormonal contraceptive with spermicidal effect, as an additive therapy to metronidazole (mnz). study population were randomly divided into 3 groups according to treatment regimen: g.i: patients received high dose mnz (2 g daily for 7 days) n = 10; g. ii: patients received (100 mg n-9 vaginal suppository daily for 7 days) n = 10; g. iii: ... | 2005 | 16083067 |
| trichomonas vaginalis p16 immunoreactivity in cervicovaginal pap tests: a diagnostic pitfall. | detection of p16 is emerging as a useful biomarker for human papillomavirus (hpv)-related dysplastic and malignant lesions of the cervix, and as such has potential application to cervicovaginal papanicolaou (pap) specimens. while evaluating p16 immunocytochemistry in our laboratory we observed one pap test in which trichomonas vaginalis stained positively for p16. we therefore proceeded to determine the frequency of t. vaginalis immunoreactivity for p16 in 10 consecutive, satisfactory, liquid-ba ... | 2005 | 16078255 |
| the epidemiology of sexually transmitted infections in adolescents. | this article addresses the epidemiology of several common sexually transmitted infections (stis) in adolescents. chlamydia is a common occurrence in adolescents, more so than is gonorrhea, but both are of particular concern because they may cause pelvic inflammatory disease. many experts recommend screening for chlamydia in sexually active adolescents, particularly females. trichomonas vaginalis is significant as a marker for risk of contracting other stis and because of its association with pre ... | 2005 | 16044389 |
| [research progress on the adhesion course of trichomonas vaginalis]. | 2005 | 16042210 | |
| [genetic characteristics of trichomonas vaginalis]. | 2005 | 16042209 | |
| [cloning and sequence analysis of trichomonas vaginalis ferredoxin gene]. | to construct a recombinant plasmid containing ferredoxin gene of trichomonas vaginalis. | 2005 | 16042202 |
| giardia mitosomes and trichomonad hydrogenosomes share a common mode of protein targeting. | mitochondria are archetypal organelles of endosymbiotic origin in eukaryotic cells. some unicellular eukaryotes (protists) were considered to be primarily amitochondrial organisms that diverged from the eukaryotic lineage before the acquisition of the premitochondrial endosymbiont, but their amitochondrial status was recently challenged by the discovery of mitochondria-like double membrane-bound organelles called mitosomes. here, we report that proteins targeted into mitosomes of giardia intesti ... | 2005 | 16040811 |
| heterogeneity in extracellular nucleotide hydrolysis among clinical isolates of trichomonas vaginalis. | trichomonas vaginalis is a parasitic protozoan that causes trichomonosis, a sexually-transmitted disease, with serious sequelae to women and men. as the host-parasite relationship is complex, it is important to investigate biochemical aspects of the parasite that contribute to our understanding of trichomonal biology and pathogenesis. nucleoside triphosphate diphosphohydrolase 1 (ntpdase 1), which hydrolyses extracellular atp and adp, and ecto-5'-nucleotidase, which hyrolyses amp, have been char ... | 2005 | 16038398 |
| frequency of infectious agents for vaginitis in non- and hysterectomized women. | the frequency of infectious agents for vaginitis has shown varying results. bacterial vaginosis and candidiasis are infections related to vaginal ph alteration. vaginal ph is related to endocervical ph in pre- and post-menopaused women, and vaginal ph in hysterectomized women is more acidic than in non-hysterectomized women. the aim of this paper is to verify differences in papanicolaou smear diagnoses of infectious agents in hysterectomized women. a retrospective study was conducted at faculdad ... | 2005 | 16021494 |
| a linear discrimination analysis based virtual screening of trichomonacidal lead-like compounds: outcomes of in silico studies supported by experimental results. | a computational (virtual) screening test to identify potential trichomonacidals has been developed. molecular structures of trichomonacidal and non-trichomonacidal drugs were represented using stochastic and non-stochastic atom-based quadratic indices and a linear discrimination analysis (lda) was trained to classify molecules regarding their antiprotozoan activity. validation tests revealed that our lda-qsar models recognize at least 88.24% of trichomonacidal lead-like compounds and suggest usi ... | 2005 | 16005626 |
| recalcitrant trichomonas vaginalis infection--a case series. | the objective of this study was (1) to estimate the prevalence of recalcitrant trichomonas vaginalis (tv) infection in a uk genitourinary medicine clinic; (2) to use a case series and literature review to suggest an algorithm for management of recalcitrant tv (rtv). a retrospective review of laboratory records, case-notes and literature review was conducted. fifteen patients were studied, representing 1.16% of the cases presenting during the study period. a wide variety of therapeutic agents was ... | 2005 | 16004634 |
| trichomonas vaginalis in a sheffield genitourinary medicine department. | the case-notes of all patients who were diagnosed with a first episode of trichomonas vaginalis (tv) between 1 october 2002 and 30 september 2003 were reviewed. a total of 78 patients were suitable for inclusion in the study. analysis of their notes revealed that, although the majority of patients presented with symptoms, 15% (n=12) of cases were asymptomatic. a raised vaginal ph was found in 94% (n=47) of the patients in whom it was measured. in all, 97% (n=76) of patients received treatment in ... | 2005 | 16004629 |
| management of metronidazole-resistant trichomonas vaginalis--a new approach. | metronidazole-resistant trichomoniasis is an infrequent but challenging problem with no universally successful treatment. since 1994, we have been using a combination regimen consisting of high-dose tinidazole plus a broad-spectrum antibiotic, i.e. doxycycline or ampicillin, and clotrimazole pessaries, for 7--14 days in this condition. a retrospective case review identified 11 cases of resistant trichomonas between 1994 and 2002. in the absence of resistance testing, resistance was defined clini ... | 2005 | 16004628 |
| predicting adolescents' longitudinal risk for sexually transmitted infection: results from the national longitudinal study of adolescent health. | influencing adolescents' sexual behaviors has the potential to influence trajectories of risk for sexually transmitted infections (stis) among young adults. | 2005 | 15997000 |
| appearance of virus-like particles in tritrichomonas foetus after drug treatment. | tritrichomonas foetus is a parasitic protist that infects the urogenital tract of cattle causing bovine trichomonosis. virus-like particles (vlps) in protozoa have been reported in several parasites including trichomonas vaginalis, a human flagellate, but viruses were never described in t. foetus so far. herein we show for the first time the presence of vlps in t. foetus after several drug treatments. they were detected by electron microscopy and were confirmed by immunofluorescence microscopy u ... | 2005 | 15979116 |
| prevalence and correlates of chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis infections, and bacterial vaginosis among a cohort of young injection drug users in baltimore, maryland. | injection drug users (idus) consistently demonstrate high-risk behaviors for sexually transmitted infections (stis). this study examines sti prevalence and correlates among young idus. | 2005 | 15976603 |
| trichomonas vaginalis-induced epithelial monolayer disruption and human immunodeficiency virus type 1 (hiv-1) replication: implications for the sexual transmission of hiv-1. | the objective of this study was to evaluate potential mechanisms of trichomonas vaginalis involvement in human immunodeficiency virus type 1 (hiv-1) transmission. polarized monolayer integrity of primary cervical and prostate epithelial cells or cell lines cultured with t. vaginalis was measured by monitoring transepithelium resistance. the effect of t. vaginalis isolates on hiv-1 passage through polarized epithelial cell monolayers was evaluated for hiv-1 p24gag in the basolateral supernatants. ... | 2005 | 15972505 |
| association between trichomoniasis and bacterial vaginosis: examination of 600 cervicovaginal smears. | to determine whether there is a relationship between trichomoniasis and bacterial vaginosis (bv), cervicovaginal smears obtained from 600 women were stained with the papanicolaou technique and examined cytologically. thirty-six (6%) of 600 women were diagnosed as having trichomonas vaginalis [tv(+)]. sixteen (44.4%) of 36 tv(+) cases were observed as having bv [bv(+)]. thirty-one of 564 tv(-) vaginal smears were also positive for bv (5.5%). there was a statistically significant correlation betwe ... | 2005 | 15969458 |
| cytologic findings in pap smears with actinomyces-like organisms. | to detect whether there is a relationship between the presence ofactinomyces-like organisms (alos) and cytologic findings. | 2005 | 15966286 |
| mucopurulent cervicitis: no longer ignored, but still misunderstood. | the last decade has produced considerable advances in the diagnosis of the common etiologies of mucopurulent cervicitis (mpc), including chlamydia trachomatis and neisseria gonorrhoeae, and in the delineation of key aspects of their pathogenesis. despite this, clear understanding of why these bacteria cause cervical inflammation in a minority of women who is infected with either organism is limited. furthermore, many women who have mpc have neither of these infections detected, even when highly ... | 2005 | 15963875 |
| a high-throughput colorimetric and fluorometric microassay for the evaluation of nitroimidazole derivatives anti-trichomonas activity. | several nitroimidazole derivatives were synthesized and tested as possible trichomonicidal agents. a fast, simple, practical and reliable in vitro colorimetric method was applied to the screening of the nitroimidazole derivatives anti-trichomonas activity. the colorimetric technique was based on the use of alamar blue as a redox-indicator. the test was carried out both qualitatively (minimal inhibitory concentration determined by naked eye observation) and quantitatively (fluorometric determinat ... | 2005 | 15963680 |
| rubrerythrin and peroxiredoxin: two novel putative peroxidases in the hydrogenosomes of the microaerophilic protozoon trichomonas vaginalis. | the parasitic flagellate trichomonas vaginalis contains hydrogenosomes, anaerobic organelles related to mitochondria, that generate atp from the fermentative conversion of pyruvate to acetate, co2 and molecular hydrogen. although an essentially anaerobic organism, trichomonas encounters low oxygen concentrations in its natural habitat and has to protect itself, and especially the oxygen-sensitve enzymes of hydrogenosomal metabolism, from oxidative damage. we have identified two novel proteins in ... | 2005 | 15904985 |
| unexpected diversity and differential success of dna transposons in four species of entamoeba protozoans. | we report the first comprehensive analysis of transposable element content in the compact genomes (approximately 20 mb) of four species of entamoeba unicellular protozoans for which draft sequences are now available. entamoeba histolytica and entamoeba dispar, two human parasites, have many retrotransposons, but few dna transposons. in contrast, the reptile parasite entamoeba invadens and the free-living entamoeba moshkovskii contain few long interspersed elements but harbor diverse and recently ... | 2005 | 15901838 |
| treatment failure of vaginal trichomoniasis in clinical practice. | we have conducted a retrospective study of treatment failure of trichomonas vaginalis (tv) infection in a busy, urban genitourinary medicine clinic in birmingham, uk, between 1998 and 2002. the prevalence of non-response to standard doses of metronidazole without any history of reinfection or nonadherence was 1.7% (28/1603) overall, and has significantly increased from 0.38% in 1999 to 3.5% in 2002 (p = 0.001, chi2 test). there were no associated demographic factors. the majority of patients (53 ... | 2005 | 15899079 |
| n-acetyl-beta-d-hexosaminidase from trichomonas vaginalis: substrate specificity and activity of inhibitors. | among chitinolytic activities previously described in trichomonas vaginalis, n-acetyl-beta-d-hexosaminidase (nahase) was the enzyme system expressing the highest level of specific activity. we report here some biochemical characteristics of nahase purified from t. vaginalis. we found at first that the use of 4-methylumbellifferyl-substrate was responsible for a substrate affinity for the enzyme, about 1000-fold higher than those when using p-nitrophenyl-substrates (pnp). whereas the optimum ph w ... | 2005 | 15890491 |
| trichomonas vaginalis: intrastrain polymorphisms within the ribosomal intergenic spacer do not correlate with clinical presentation. | trichomoniasis presents a broad spectrum of clinical patterns ranging from asymptomatic to severe vaginitis and cervicitis. despite its importance, very little is known about the genetic relatedness of its causative agent, trichomonas vaginalis, and the clinical phenotypes. to address this question, analysis of restriction length polymorphism (rflp) within the intergenic spacer of the ribosomal dna (igs) from 60 clinically defined isolates of t. vaginalis was performed. this is the first descrip ... | 2005 | 15888292 |
| trichomonas vaginalis adherence mediates differential gene expression in human vaginal epithelial cells. | trichomonas vaginalis, an ancient protist, colonizes the vaginal mucosa causing trichomonosis, a vaginitis that sometimes leads to severe health complications. preparatory to colonization of the vagina is the adhesion to vaginal epithelial cells (vecs) by trichomonads. we hypothesized that vecs alter the gene expression to form a complex signalling cascade in response to trichomonal adherence. in order to identify the genes that are upregulated, we constructed a subtraction cdna library after co ... | 2005 | 15888089 |
| bacterial vaginosis and intravaginal practices: association with hiv. | to determine the prevalence of bacterial vaginosis and to identify risk factors associated with this condition among urban women in harare, zimbabwe. | 2004 | 15881309 |
| the diagnosis of trichomonas vaginalis in liquid-based pap tests: correlation with pcr. | the conventional papanicolaou smear (cps) is not considered accurate for the diagnosis of trichomonas vaginalis (t. vaginalis), and women noted to carry the organism on cps are recommended to undergo confirmatory testing. liquid-based preparations have been shown to facilitate the diagnosis of squamous lesions and may also facilitate the diagnosis of t. vaginalis. we used polymerase chain reaction (pcr) to investigate the accuracy of the diagnosis of t. vaginalis by the liquid-based pap test (lb ... | 2005 | 15880709 |
| increased sensitivity of trichomonas vaginalis isolation from vaginal secretions by subsequent blind passage of preliminary negative cultures. | trichomonas vaginalis is a vaginal protozoon causing the most prevalent nonviral sexually transmitted disease. culture is considered as the most reliable and gold standard among conventional diagnostic methods in the detection of t. vaginalis. in the present study, the diagnostic effect of subsequent blind culturing of vaginal secretion samples in diamond's complete medium at every 48 h was investigated in 93 women. vaginal secretion samples were examined by wet mount and inoculated into diamond ... | 2005 | 15878448 |
| measuring the significance of field validation in the college of american pathologists interlaboratory comparison program in cervicovaginal cytology: how good are the experts? | expert opinion is often used as a gold standard for gynecologic cytology in the evaluation of new technologies, in the legal setting, and in the validation of cases for use in educational programs and proficiency testing. however, the reliability of expert opinion alone in selecting slides of a specific cytodiagnosis that can be reproducibly and reliably identified by subsequent reviewers has not been determined. | 2005 | 15859630 |
| trichomonas vaginalis: identification of soluble and membrane-associated phospholipase a1 and a2 activities with direct and indirect hemolytic effects. | a direct hemolytic activity, dependent on phospholipase a (pla) activity, was located in the particulate subcellular fraction (p30) of trichomonas vaginalis. we identified soluble direct and indirect hemolytic activities in the spent medium and soluble fraction (s30) of t. vaginalis strain gt-13. spent medium showed the highest specific indirect hemolytic activity (siha) at ph 6.0 (91 indirect hemolytic units [hu]/mg/hr). spent medium and p30, but not s30, showed direct hemolytic activity. pla a ... | 2005 | 15856864 |
| rifaximin, a peculiar rifamycin derivative: established and potential clinical use outside the gastrointestinal tract. | rifaximin is a poorly absorbed semisynthetic rifamycin derivative with a broad spectrum of antibacterial activity including gram-positive and gram-negative bacteria, both aerobes and anaerobes. although originally developed for the treatment of infectious diarrhea, the appreciation of the pathogenic role of gut bacteria in several organic and functional gastrointestinal diseases has increasingly broadened its clinical use. the availability of a topical formulation (a cream containing 5% of the d ... | 2005 | 15855757 |
| [frequency of trichomonas vaginalis infection in couples with fertility problems]. | one-hundred and seventy two couples which went for the first time to the infertility service of the national institute of endocrinology from june 1999 to june 2000, were studied to find out the frequency of trichomonas vaginalis infection in this group, and determine its interaction with a number of clinical and risk variables. the results yielded that 10.5% were positive to the parasite, the prevailing symptom was leukorrea in women and 96.6% of men showed no symptoms. it was highly significant ... | 2002 | 15849932 |
| [behavior of vaginal trichomoniasis in a group of adolescents]. | 275 adolescents aged 10-19 receiving attention at the infantojuvenile department of pregnancy interruption and of sexually transmitted infections of "ramón gonzalez coro" and "eusebio hernández" gynecoobstetric teaching hospitals, both in havana city, were studied from august, 1999, to september, 2000, aimed at knowing the behavior of the infection caused by trichomonas vaginalis in this population group. the results yielded 18.1 % of positivity to parasitosis. it was proved by the calculation o ... | 2003 | 15849923 |
| synthesis and antiprotozoan evaluation of new alkyl-linked bis(2-thioxo-[1,3,5]thiadiazinan-3-yl) carboxylic acids. | two new series of several alkyl-linked bis(2-thioxo-[1,3,5]thiadiazinan-3-yl) carboxylic acids were synthesized in a two step procedure from the corresponding alkyl bis-dithiocarbamic salt intermediary. the novel compounds were evaluated for their activity in vitro against trypanosoma cruzi strain cl (clone cl b5) and trichomonas vaginalis strain jh 31a. | 2005 | 15848753 |
| [application of an alternative method to counting in neubauer chamber to determine trichomonas vaginalis concentrations]. | it was demonstrated that there is a relation of cell concentration with the parasite reading in an elisa reader. it was determined that absorbance showed significant values in wavelengths of visible range and the possible minimum wavelength (450 nm)was chosen to guarantee the maximum sensitivity. likewise, it was confirmed that absorbance significantly increased (p < 0.001) after the plate was filled up, it stabilized in the 40m-2h time interval when applying 100, 150, 200 or 300 ml per well of ... | 2001 | 15846921 |
| is a change in the vaginal flora associated with an increased risk of preterm birth? | the purpose of this study was to determine if a change in the vaginal flora was associated with an increased risk of preterm birth, and to determine if metronidazole therapy before 32 weeks increased the risk of preterm birth. | 2005 | 15846235 |
| trichomonas vaginalis polyamine metabolism is linked to host cell adherence and cytotoxicity. | trichomonas vaginalis secretes putrescine that is readily detected in vaginal secretions. we wanted to examine the effect of decreased putrescine synthesis by inhibition of ornithine decarboxylase (odc) on t. vaginalis. one reason is because inhibition of tritrichomonas foetus odc results in growth arrest, destruction of hydrogenosomes, and decreased amounts of hydrogenosomal enzymes. treatment of t. vaginalis t016 with >/=20 mm 1,4-diamino-2-butanone (dab) to inhibit odc resulted in growth arre ... | 2005 | 15845462 |
| identification of trichomonas vaginalis cysteine proteases that induce apoptosis in human vaginal epithelial cells. | a secreted cysteine protease (cp) fraction from trichomonas vaginalis is shown here to induce apoptosis in human vaginal epithelial cells (hvec) and is analyzed by mass spectrometry. the trichomonad parasite t. vaginalis causes one of the most common non-viral sexually transmitted infection in humans, trichomoniasis. the parasite as well as a secreted cysteine protease (cp) fraction, isolated by affinity chromatography followed by bio-gel p-60 column chromatography, are shown to induce hvec apop ... | 2005 | 15843376 |
| clinical presentation and management of alleged sexually assaulted females at mulago hospital, kampala, uganda. | to determine the presentation and treatment offered to sexually assaulted females attending emergency gynaecological ward in mulago hospital, kampala, uganda. | 2005 | 15843131 |
| increased prevalence of leukocytes and elevated cytokine levels in semen from schistosoma haematobium-infected individuals. | in this study, we investigated the seminal inflammatory response to egg infestation of the urogenital organs in 240 semen-donating men aged 15-49 years living in a schistosoma haematobium-endemic area of madagascar. in 29 subjects (12%) with excretion of > or =5 ova/ejaculate, leukocytospermia (>10(6) leukocytes/ml) and the presence of seminal lymphocytes and eosinophil leukocytes were each significantly more prevalent than in 74 subjects (31%) who were s. haematobium negative (p<.01). in additi ... | 2005 | 15838790 |
| [routine examination of leukorrhea in 3347 patients with trichomonal vaginitis]. | 2004 | 15830896 | |
| molecular cloning and characterization of a rac1 homologue cdna from trichomonas vaginalis. | to clone and characterize a rac1 homologue from trichomonas vaginalis for studying cell cycle of the organism. | 2004 | 15830884 |
| [study on surface adhesion protein 33 gene sequence of different trichomonas vaginalis isolates]. | to study genetic polymorphism of surface adhesion protein 33 (ap33) gene on the seven isolates of trichomonas vaginalis. | 2004 | 15830862 |
| in vitro resistance of neisseria gonorrhoeae to metronidazole. | susceptibility of 64 clinical isolates of neisseria gonorrhoeae to metronidazole was determined by the agar dilution technique using mueller-hinton and chocolate agar media. all isolates were resistant to metronidazole at 12.5 mug/ml in either medium. this lack of activity of metronidazole against n. gonorrhoeae would suggest that the ability to recover n. gonorrhoeae in patients being treated with metronidazole for concurrent trichomonas vaginalis infections should not be hampered. | 1974 | 15830482 |
| the diagnosis of trichomonas vaginalis in liquid-based pap tests: morphological characteristics. | we investigated the morphological features of trichomonas vaginalis in liquid-based papanicolaou (pap) (lbp) tests, in order to assess the impact of this new slide preparation system on recognition of t. vaginalis organisms. we reviewed 88 lbp test slides previously interpreted as showing t. vaginalis and assessed morphological characteristics of the organisms and the presence of secondary features. eighty-six of 88 slides showed, at least focally, organisms with cytoplasmic granules, nuclei, an ... | 2005 | 15830365 |
| effect of piroxicam, metamizol, and s-adenosylmethionine in a murine model of experimental trichomoniasis. | biological effects of piroxicam, metamizol, and s-adenosylmethionine (s-amet) have been tested in nmri mice infected intraperitoneally with trichomonas vaginalis. an intraperitoneal treatment during ten preinfection days with piroxicam (10 mg/kg/day), or metamizol (275 mg/kg/day), but not with s-amet (117 mg/kg/day) induced a significant decrease of abdominal lesions and mortality, assessed by means of a pathogenicity index. the trichomonicidal activity of piroxicam, metamizol, and s-amet was te ... | 2005 | 15828587 |
| identification of a subversive substrate of trichomonas vaginalis purine nucleoside phosphorylase and the crystal structure of the enzyme-substrate complex. | trichomonas vaginalis is an anaerobic protozoan parasite that causes trichomoniasis, a common sexually transmitted disease with worldwide impact. one of the pivotal enzymes in its purine salvage pathway, purine nucleoside phosphorylase (pnp), shows physical properties and substrate specificities similar to those of the high molecular mass bacterial pnps but differing from those of human pnp. while carrying out studies to identify inhibitors of t. vaginalis pnp (tvpnp), we discovered that the non ... | 2005 | 15817485 |
| seroepidemiology of trichomonas vaginalis in rural women in zimbabwe and patterns of association with hiv infection. | serological assays using dried blood spots from 5221 women in rural areas of eastern zimbabwe were used to assess the epidemiology of trichomonas vaginalis infection, and its association with hiv. antibodies to t. vaginalis and to hiv were detected by enzyme immunoassays. behavioural and demographic data were collected by confidential questionnaires. in total, 516 (9.9%) women were seropositive for t. vaginalis and seroprevalence increased with age among younger women. divorced, widowed and sing ... | 2005 | 15816157 |
| structural implications of novel diversity in eucaryal rnase p rna. | previous eucaryotic rnase p rna secondary structural models have been based on limited diversity, representing only two of the approximately 30 phylogenetic kingdoms of the domain eucarya. to elucidate a more generally applicable structure, we used biochemical, bioinformatic, and molecular approaches to obtain rnase p rna sequences from diverse organisms including representatives of six additional kingdoms of eucaryotes. novel sequences were from acanthamoeba (acathamoeba castellanii, balamuthia ... | 2005 | 15811915 |
| synthesis and biological properties of new 5-nitroindazole derivatives. | a series of new 3-alkoxy- or 3-hydroxy-1-[omega-(dialkylamino)alkyl]-5-nitroindazoles have been synthesized and their trichomonacidal, antichagasic and antineoplastic properties studied. five derivatives (5, 6, 8, 9 and 17) showed remarkable trichomonacidal activity against trichomonas vaginalis at 10 microg/ml concentration. three compounds (8, 10, 11) exhibited interesting antichagasic activity and these same compounds moderate antineoplastic activity against tk-10 and ht-29 cell lines. unspec ... | 2005 | 15809155 |
| sexually transmitted infections among pregnant women attending antenatal clinics in mongolia: potential impact on the mongolian hiv epidemic. | we conducted mongolia's first nation-wide cross-sectional survey of sexually transmitted infections (stis) among pregnant women attending prenatal care. among our 2000 participants, 386 (19.3%) were infected with chlamydia trachomatis, 133 (6.7%) with trichomonas vaginalis, 121 (6.1%) with neisseria gonorrhoeae, and 128 (6.4%) were seropositive for treponemal antibodies. none of our participants were seropositive for hiv infection. additionally, 605 (30.3%) of the women had at least one sti, 133 ... | 2005 | 15807944 |
| antibiotics for bacterial vaginosis or trichomonas vaginalis in pregnancy: a systematic review. | to determine whether antibiotic treatment for bacterial vaginosis or trichomonas vaginalis during pregnancy decreases the risk of preterm birth and associated adverse outcomes. | 2005 | 15802417 |
| collecting the essence of man: semen collection for hiv transmission studies in sub-saharan africa. | 2005 | 15800108 | |
| clinically resistant trichomoniasis. | 2005 | 15800103 | |
| the prevalence of chlamydia, gonorrhea, and trichomonas in sexual partnerships: implications for partner notification and treatment. | treatment of sex partners by patient-delivered partner therapy (pdpt) may prove to be an effective strategy in reducing reinfection and preventing the sequelae of sexually transmitted infections (stis). however, limited data exists regarding stis within sexual partnerships (dyads). | 2005 | 15788928 |
| vaginal swabs versus lavage for detection of trichomonas vaginalis and bacterial vaginosis among hiv-positive women. | cervicovaginal lavage (cvl) is often used for research and may be easier and more accurate than vaginal swabs as a specimen collection method. | 2005 | 15788920 |
| nematocidal and trichomonacidal activities of 2-substituted quinolines. | several quinolines were synthesized and evaluated in vitro and in vivo against the nematodes caenorhabditis elegans, heligmosomoides polygyrus and the protozoa trichomonas vaginalis. if some of them have shown in vitro nematocide activity (at 10 microm), however, their trichomonacidal activity reached 50% reduction at only 100 microm. the in vivo activity on trichinella spiralis model was evaluated for some of the most in vitro active quinolines. | 2005 | 15784240 |
| prevalence of candida albicans and trichomonas vaginalis in pregnant women in havana city by an immunologic latex agglutination test. | we aimed to estimate the prevalence of candida albicans and trichomonas vaginalis in immunocompetent pregnant women living in havana city, cuba, with or without symptoms of vaginitis, using a sample of 640 women from 6 gyneco-obstetrics hospitals, which represents 2.5% of total yearly pregnant women. diagnosis was made using a new latex agglutination kit (newvagin c-kure, la habana, cuba). clinical sensitivity and specificity of this assay were validated against culture method, with 467 and 489 ... | 2004 | 15775877 |
| is the presence of trichomonas vaginalis a reliable predictor of coinfection with chlamydia trachomatis and/or neisseria gonorrhoeae in female ed patients? | the aim of the study was to determine if the presence of trichomonas vaginalis (tv) is associated with an increased risk of coinfection with chlamydia trachomatis (ct) and/or neisseria gonorrhoeae (ng) in female patients presenting to the ed with symptoms consistent with a sexually transmitted disease (std). | 2005 | 15765329 |
| spliceosomal introns in the deep-branching eukaryote trichomonas vaginalis. | eukaryotes have evolved elaborate splicing mechanisms to remove introns that would otherwise destroy the protein-coding capacity of genes. nuclear premrna splicing requires sequence motifs in the intron and is mediated by a ribonucleoprotein complex, the spliceosome. here we demonstrate the presence of a splicing apparatus in the protist trichomonas vaginalis and show that rna motifs found in yeast and metazoan introns are required for splicing. we also describe the first introns in this deep-br ... | 2005 | 15764705 |
| [frequency of vaginal infections in pregnant women in the department of perinatology and gynaecology in zabrze]. | the analysis of vaginal infections in pregnant women admitted to the department of perinatology and gynaecology in zabrze, from january 2001 to september 2003. | 2004 | 15751213 |
| heterologous expression in tritrichomonas foetus of functional trichomonas vaginalis ap65 adhesin. | trichomonosis, caused by trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. the interaction of t. vaginalis with vaginal epithelial cells (vecs), a step preparatory to infection, is mediated in part by the prominent surface protein ap65. the bovine trichomonad, tritrichomonas foetus, adheres poorly to human vecs. thus, we established a transfection system for heterologous expression of the t. vagin ... | 2005 | 15748280 |
| effects of bacterial vaginosis and other genital infections on the natural history of human papillomavirus infection in hiv-1-infected and high-risk hiv-1-uninfected women. | whether the natural history of human papillomavirus (hpv) infection is affected by bacterial vaginosis (bv) or trichomonas vaginalis (tv) infection has not been adequately investigated in prospective studies. | 2005 | 15747249 |
| evolutionary biology: the hydrogenosome's murky past. | 2005 | 15744282 |