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osmotic, biomass, and oxygen effects on the growth rate of fusarium oxysporum using a dissolved-oxygen-controlled turbidostat.the system described is a modified hospodka's turbidostat. this device helps to measure the maximum growth rate of fungi in steady-state aerobic conditions with defined and independent concentration of dissolved oxygen, biomass, and substrate even unlimited. the principle consists of a turbidostat controlled by the dissolved oxygen concentration. the inlet medium pump operates when the dissolved oxygen concentration falls below the set point value. this method allows us to study independently ef ...198918587971
antimicrobial activity of globulol isolated from the fruits of eucalyptus globulus labill.an antimicrobial sesquiterpene was separated by bioassay-guided isolation from the petroleum ether fraction of the ethanol crude extract of eucalyptus globulus labill (myrtaceae) fruits, and was identified as globulol by physicochemical properties and spectroscopic analysis. mycelial growth method revealed that the median effective inhibitory concentration (ic50) values of globulol on alternaria solani, fusarium oxysporum f.sp. niverum, f. graminearum, rhizoctonia solani and venturia pirina were ...200818569693
myd88 regulation of fusarium keratitis is dependent on tlr4 and il-1r1 but not tlr2.the fungal pathogens fusarium solani and fusarium oxysporum cause severe corneal disease in the united states and worldwide and were the causative organisms in a recent outbreak of contact lens-associated keratitis. to characterize innate immunity in fusarium keratitis, we developed a murine model in which conidia are injected into the corneal stroma. immunocompetent c57bl/6 mice rapidly developed severe corneal opacification associated with neutrophil infiltration and clearance of fusarium hyph ...200818566426
characterization of a new isolate of pseudomonas fluorescens strain psd as a potential biocontrol agent.evaluation of a new isolate of pseudomonas fluorescens for its biocontrol properties.200818565138
cloning and characterization of a novel chitinase gene (chi46) from chaetomium globosum and identification of its biological activity.chitinases play a major role in the defensive strategies of plants against fungal pathogens. in the current study, the gene for a 46-kda endochitinase (chi46) was cloned from chaetomium globosum, an important biocontrol fungus. the corresponding complementary deoxyribonucleic acid sequence was 1,350 bp in length, encoding 449 amino acid residues. the temporal expression of chi46, in response to the treatments of cell walls of six pathogens and confrontation against two fungal pathogens, was meas ...200818563407
optimization of r-(+)-alpha-terpineol production by the biotransformation of r-(+)-limonene.r-(+)-limonene is an abundant and non-expensive by-product of the citrus industry and is, therefore, a suitable starting material for the production of natural flavor and fragrance compounds. the biotransformation of r-(+)-limonene to r-(+)-alpha-terpineol by fusarium oxysporum 152b has already been reported, although the influence of the main process parameters on the production has not yet been evaluated. in this paper, a plackett-burman screening design was used to define the effects of the m ...200818560915
isolation and identification of antifungal fatty acids from the basidiomycete gomphus floccosus.bioautography of extracts of the fruiting bodies of the basidiomycete gomphus floccosus (schw.) singer indicated the presence of fungitoxic compounds in the ethyl acetate fraction against the plant pathogens colletotrichum fragariae, colletotrichum gloeosporioides, and colletotrichum acutatum. bioassay-guided fractionation of this fraction resulted in the isolation of the bioactive fatty acids (9 s,10 e,12 z)-9-hydroxy-10,12-octadecadienoic acid (1), (9 e,11 z)-13-oxo-9,11-octadecadienoic acid ( ...200818557621
isolation and partial characterization of bacillus subtilis me488 for suppression of soilborne pathogens of cucumber and pepper.environmentally friendly control measures are needed for suppression of soilborne pathogens of vegetable crops in the republic of korea. in vitro challenge assays were used to screen approximately 500 bacterial isolates from 20 korean greenhouse soils for inhibition of diverse plant pathogens. one isolate, bacillus subtilis me488, suppressed the growth of 39 of 42 plant pathogens tested. isolate me488 also suppressed the disease caused by fusarium oxysporum f. sp. cucumerinum on cucumber and phy ...200818542950
chemical composition and antifungal activity of essential oil and various extract of silene armeria l.the aims of this study were to examine the chemical composition of the essential oil isolated from the floral parts of silene armeria l. by hydrodistillation, and to test the efficacy of essential oil and various leaf extracts (n-hexane, chloroform, ethyl acetate and methanol) as an antifungal potential. the gc-ms analysis determined that 28 compounds, which represented 89.03% of total oil, were present in the oil containing mainly 1-butene, methylcyclopropane, 2-butene and caryophyllene oxide. ...200818538562
the complete mitochondrial genome of fusarium oxysporum: insights into fungal mitochondrial evolution.the complete mitochondrial dna (mtdna) sequence was determined for the phytopathogenic fungus fusarium oxysporum. it is 34,477 bp long, maps circularly, and encodes for 14 protein-coding, 25 trna and 2 rrna genes. the nucleotide and amino acid data sets from its 14 concatenated protein-coding mitochondrial (mt) genes were used along with gene order comparisons for an extensive phylogenetic study of the subphylum pezizomycotina. our results are in agreement with current taxonomic treatments and a ...200818538510
possibilities of the use of vinasses in the control of fungi phytopathogens.the purpose of this research was to study the biocide effect of three agroindustrial subproducts, concretely sugar beet, sugar cane and wine vinasse. results from in vitro testing determined that wine vinasse is what shows a 100% capacity to suppress fungal growth with concentrations between 5% and 7% for fusarium oxysporum f.sp. melonis race 0 and 1, sclerotinia sclerotiorum, pythium aphanidermatum and phytophthora parasitica and 10-15% for f. oxysporum f.sp. radicis-cucumerinum. on the other h ...200818519163
role of the white collar 1 photoreceptor in carotenogenesis, uv resistance, hydrophobicity, and virulence of fusarium oxysporum.knockout mutants of fusarium oxysporum lacking the putative photoreceptor wc1 were impaired in aerial hyphae, surface hydrophobicity, light-induced carotenogenesis, photoreactivation after uv treatment, and upregulation of photolyase gene transcription. infection experiments with tomato plants and immunodepressed mice revealed that wc1 is dispensable for pathogenicity on plants but required for full virulence on mammals.200818503005
caspofungin-mediated beta-glucan unmasking and enhancement of human polymorphonuclear neutrophil activity against aspergillus and non-aspergillus hyphae.we investigated whether caspofungin and other echinocandins have immune-enhancing properties that influence human polymorphonuclear neutrophil (pmn)-mediated mold hyphal damage.200818500936
genetic relatedness of trichoderma isolates antagonistic against fusarium oxysporum f.sp. dianthi inflicting carnation wilt.twenty-eight isolates of trichoderma belonging to four different species were screened in vitro for their antagonistic ability against fusarium oxysporum f.sp. dianthi causing carnation wilt. three different levels of antagonism observed in dual plate assay were further confirmed by cell-free culture filtrate experiments. isolates showing class i level of antagonism produced maximum lytic enzymes, chitinases and beta-1,3-glucanases. genetic variability of 25 selected isolates was assessed by ran ...200818500632
citrus compost and its water extract for cultivation of melon plants in greenhouse nurseries. evaluation of nutriactive and biocontrol effects.two different types of citrus composts, and their water extracts, were tested with regard to their utilisations as partial substitutes for peat in growing media for melon seedlings in greenhouse nurseries. both compost showed higher plant growth than peat. compost composed by citrus waste and green residue (c2) showed greater plant growth than compost obtained from the same organic matrices mentioned above further the addition of sludge obtained from citrus industry (c1). compost c2 showed a gre ...200818499444
response of endophytic bacterial communities in banana tissue culture plantlets to fusarium wilt pathogen infection.endophytic bacteria reside within plant hosts without having pathogenic effects, and various endophytes have been found to functionally benefit plant disease suppressive ability. in this study, the influence of banana plant stress on the endophytic bacterial communities, which was achieved by infection with the wilt pathogen fusarium oxysporum f. sp. cubense, was examined by cultivation-independent denaturing gradient gel electrophoresis analysis of 16s ribosomal dna directly amplified from plan ...200818497482
efficacy of organophosphorus derivatives containing chalcones/chalcone semicarbazones against fungal pathogens of sugarcane.ten newly synthesized organophosphorus derivatives containing substituted chalcones and substituted chalcone semicarbazones were tested for their antifungal efficacy against colletotrichum falcatum, fusarium oxysporum, curvularia pallescens (all sugarcane pathogens). the o,o-diethylphosphate derivatives containing 2-chlorochalcone and 2-chlorochalcone semicarbazone exhibited 70-85% mycelial inhibition against all the test fungi at 1000 ppm. the screening results were correlated with structural f ...200218476009
genome flux in tomato cell clones cultured in vitro in different physiological equilibria. ii. a rapd analysis of variability.an analysis of the effect of changing physiological conditions on genome evolution in tomato cell populations has been carried out on long-term in vitro cultured clones grown on different auxin-cytokinin equilibria or selected for low-high competence for active defense against fusarium oxysporum f.sp. lycopersici. rapd analysis, confirmed through pattern rehybridization, was used as a random tool to measure the genetic variability. through the use of a modified anova, variation was shown to depe ...199618469940
suppression of plant resistance gene-based immunity by a fungal effector.the innate immune system of plants consists of two layers. the first layer, called basal resistance, governs recognition of conserved microbial molecules and fends off most attempted invasions. the second layer is based on resistance (r) genes that mediate recognition of effectors, proteins secreted by pathogens to suppress or evade basal resistance. here, we show that a plant-pathogenic fungus secretes an effector that can both trigger and suppress r gene-based immunity. this effector, avr1, is ...200818464895
impact of contact lens group on antifungal efficacy of multipurpose disinfecting contact lens solutions.to evaluate and compare the in vitro effectiveness of multipurpose contact lens solutions (mpds) to inhibit fungal colonization of food and drug administration (fda) contact lens groups.200818463480
isolation of two antifungal compounds fromneolitsea cuipala and their activity against some plant pathogenic fungi.zeylanidine and zeylanicine isolated fromneolitsea cuipala fruit peels are found to be potential antifungal agents againsthelminthosporium oryzae, fusarium oxysporum, alternaria solani, curvularia varuciformis andcolletotrichum gleosporioides.199918461489
the novel cladosporium fulvum lysin motif effector ecp6 is a virulence factor with orthologues in other fungal species.during tomato leaf colonization, the biotrophic fungus cladosporium fulvum secretes several effector proteins into the apoplast. eight effectors have previously been characterized and show no significant homology to each other or to other fungal genes. to discover novel c. fulvum effectors that might play a role in virulence, we utilized two-dimensional polyacrylamide gel electrophoresis (2d-page) to visualize proteins secreted during c. fulvum-tomato interactions. three novel c. fulvum proteins ...200818452583
influence of the chloride channel of fusarium oxysporum on extracellular laccase activity and virulence on tomato plants.clc-type voltage-gated chloride channels are a family of proteins which mediate chloride transport across the plasma and intracellular membranes. a clc1 gene from the vascular wilt fungus fusarium oxysporum was characterized and disrupted. the predicted clc1 protein contained highly conserved transmembrane and cbs domains of this protein family and showed significant identities to the saccharomyces cerevisiae gef1 and the cryptococcus neoformans clc-a chloride channels. inactivation of clc1 caus ...200818451056
biological control of phytopathogenic fungi by fatty acids.the aim of the present study was to evaluate the antifungal activity of fatty acids against phytopathogenic fungi. two pot experiments were conducted by mixing palmitic and oleic acids in the soil in which poor plant growth was observed. in addition, the antifungal activities of nine fatty acids (butyric acid, caproic acid, caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, oleic acid, and linoleic acid) against four phytopathogenic fungi: alternaria solani, colletotrichum la ...200818443921
biocontrol strain pseudomonas fluorescens wcs365 inhibits germination of fusarium oxysporum spores in tomato root exudate as well as subsequent formation of new spores.fusarium oxysporum f.sp.radicis-licopersici (forl) is a soilborne pathogenic fungus which can cause tomato foot and root rot (tfrr). tomato root exudate is a good source of nutrients for both forl and the tfrr-suppressing biocontrol bacterium pseudomonas fluorescens strain wcs365. incubation of forl microconidia in tomato root exudate stimulates their germination. this phenomenon is observed, to a lesser extent, upon incubation in plant nutrient solution supplemented with citrate or glucose, the ...200818430156
the influence of various carbon and nitrogen sources on oil production by fusarium oxysporum.the oil-synthesizing capacity of fusarium oxysporum, cultivated on basal nutrient medium, was evaluated using different carbon and nitrogen sources. in one of the media, molasses was also used as a principal carbon source. media containing glucose and ammonium nitrate were found to be most efficient for oil production. fatty acid profile of the fungal oil indicated the presence of a wide range of fatty acids ranging from c8 to c24. fatty acid composition largely depends on the type of carbon and ...198718425683
cloning, characterization and functional expression of an alkalitolerant type c feruloyl esterase from fusarium oxysporum.a hypothetical protein fofaec-12213 of fusarium oxysporum was found to have high amino acid sequence identity with known type c feruloyl esterases (faes) containing a 13-amino acid conserved region flanking the characteristic g-x-s-x-g motif of a serine esterase. the putative fae from the genomic dna was successfully cloned in frame with the saccharomyces cerevisiae alpha-factor secretion signal under the transcriptional control of the alcohol oxidase (aox1) promoter and integrated in pichia pas ...200818414848
cloning and characterization of a novel lpwrky1 transcription factor in tomato.the initiation of defence responses in plants is accompanied by fundamental changes in gene expression: the expression of pathogenesis-related genes is co-ordinately regulated with metabolic changes such as down regulation of photosynthesis and induction of sink metabolism. to identify candidate regulators of this co-ordinated regulatory mechanism, the role of wrky transcription factors in the initiation of defence response was analysed in tomato. a wrky-type transcription factor (lpwrky1) from ...200818406156
synthesis, characterization and electrochemistry of 4'-functionalized 2,2':6',2''-terpyridine ruthenium(ii) complexes and their biological activity.the synthesis and characterization of ru(ii) terpyridine complexes derived from 4'-functionalized 2,2':6',2''-terpyridine ligands by a multi step procedure have been described. the complexes are redox-active, showing both metal-centred (oxidation) and ligand-centred (reduction) processes. the antibacterial and antifungal activity of the synthesized ruthenium(ii) complexes [ru(attpy)2](pf6)2 (attpy = 4'-(4-acryloyloxymethylphenyl)-2,2':6',2''-terpyridine); [ru(mttpy)2](pf6)2 (mttpy = 4'-(4-methac ...200818398539
bacterial ectosymbionts and virulence silencing in a fusarium oxysporum strain.in the present article we have ascertained the presence of a consortium of ectosymbiotic bacteria belonging to serratia, achromobacter, bacillus and stenotrophomonas genera associated to the mycelium of the antagonistic fusarium oxysporum msa 35 [wild-type (wt) strain]. morphological characterization carried out on the wt strain, on the f. oxysporum msa 35 without ectosymbionts [cured (cu) strain] and on the pathogenic f. oxysporum f.sp. lactucae (fuslat 10) showed that the ectosymbionts, presen ...200818397306
wilting of date palm branches by fusarium oxysporum in south of iran and its control managements with soil solarization method.wilting of some branches in nurseries and orchards of date palm were studied in south of iran including ahvaz and abadan cities in 2005-2006 years. different infected plants were visited and samples showing symptoms including wilting or death of branches collected from various areas and transferred to laboratory. samples were cultured in common media (pda) and different fungi were studied and identified. the most frequently isolated pathogen was fusarium oxysporum which caused wilting of some br ...200718396818
differential interaction between melon cultivars and race 1.2 of fusarium oxysporum f.sp. melonis.one hundred and ten melon accessions from different origins were evaluated for resistance to race 1.2 of fusarium oxysporum f.sp. melonis (fom). artificial inoculations were performed and plants were scored for presence or absence of symptoms. the screening revealed that sources of resistance to this fungus are limited. however, a few resistant accessions were found. the accessions 'kogane nashi makuwa', 'c-211' and 'c-40' from japan provided the highest resistance to both patotypes of race 1.2 ...200718396817
introduction a potato cultivar "sprit" as relatively resistant to main fungal pathogens causal agents of early blight and wilting on potato in iran.potato (solanum tubersum l.) is one of the most human food production cultured in iran especially zanjan province as a temperate region. some fungal pathogens caused severely infected on potato tubers or foliage in the majority grown areas and resulted yield losses in potato production. recent years from 2002 to 2004 infected samples were collected from different potato grown regions in zanjan province then cultured on pda after surface sterilization with sodium hypochlorite. isolated fungal pat ...200718396814
yield loss in susceptible cultivars of spring rapeseed due to fusarium wilt caused by fusarium oxysporum.in 1999, reports of spring rapeseed plants (brassica napus l.) exhibiting wilt symptoms were received by agricultural extension personnel from farmers near fort vermillion and andrew, alberta, canada. fungal colonies recovered from affected plants after surface disinfection were identified as fusarium oxysporum by comparison of morphology on carnation leaf and potato dextrose agars with literature descriptions and reference cultures. root-dip inoculation of young rapeseed plants with spore suspe ...200718396801
susceptibility of gerbera and chrysanthemum varieties (gerbera jamesoni and chrysanthemum morifolium) to fusarium oxysporum f.sp. chrysanthemi.in 2002, gerbera plants (cv kaliki) were observed exhibiting symptoms of a wilt in a soilless cultivation at albenga area (northern italy). a similar wilt was also observed in the sanremo area (northern italy) on cv red bull, anedin and gud finger grown in soil. the same observations were carried out in 2004 in sw spain where gerbera plants showing wilt symptoms were observed in soilless crops. in all cases, the planting material originated from the netherlands. recently on the base of experimen ...200718396800
molecular characterization of cell death induced by a compatible interaction between fusarium oxysporum f. sp. linii and flax (linum usitatissimum) cells.the cellular and molecular events associated with cell death during compatible interaction between fusarium oxysporum sp. linii and a susceptible flax (linum usitatissimum) cell suspension are reported here. in order to determine the physiological and molecular sequence of cell death of inoculated cells, reactive oxygen species (ros) production, mitochondrial potential, lipoxygenase, dnase, protease and caspase-3-like activities, lipid peroxidation and secondary metabolite production were monito ...200818396055
cytotoxicities of enniatins h, i, and mk1688 from fusarium oxysporum kfcc 11363p.enniatins (ens) h, i, and mk1688 and beauvericin (bea) were purified from concentrated chloroform extracts of fusarium oxysporum kfcc 11363p submerged cultures using hplc, and their in vitro cytotoxicities were evaluated against four human carcinoma cell lines (lung, a549; ovarian, sk-ov-3; skin melanoma, sk-mel-2; and colon, hct15) using the sulforhodamine b (srb) method. ens i and mk1688 inhibited the growth of cancer cell lines most strongly and had similar cytotoxic effects on the tested hum ...200818394672
a fumonisin biosynthetic gene cluster in fusarium oxysporum strain o-1890 and the genetic basis for b versus c fumonisin production.most species of fusarium that produce fumonisin mycotoxins produce predominantly b fumonisins (fbs). however, fusarium oxysporum strain o-1890 produces predominantly c fumonisins (fcs). in this study, the nucleotide sequence of the fumonisin biosynthetic gene (fum) cluster in strain o-1890 was determined. the order and orientation of fum genes were the same as in the previously described clusters in fusarium verticillioides and fusarium proliferatum. coding regions of f. oxysporum and f. vertici ...200818375156
microfungal "weeds" in the leafcutter ant symbiosis.leafcutter ants (formicidae: tribe attini) are well-known insects that cultivate basidiomycete fungi (agaricales: lepiotaceae) as their principal food. fungus gardens are monocultures of a single cultivar strain, but they also harbor a diverse assemblage of additional microbes with largely unknown roles in the symbiosis. cultivar-attacking microfungi in the genus escovopsis are specialized parasites found only in association with attine gardens. evolutionary theory predicts that the low genetic ...200818369523
synthetic and spectroscopic characterization of organotin(iv) complexes of biologically active schiff bases derived from sulpha drugs.a number of diorganotin(iv) complexes with schiffbase have been synthesized and characterized by elemental analysis, conductance measurements, molecular weight determinations, infrared, electronic and multinuclear magnetic resonance ((1)h, (13)c and (119)sn nmr) spectral data. the molar conductivity data shows non-electrolytic nature of complexes. the bidentate nature of the ligands is inferred from ir and nmr spectral studies. the antimicrobial activities of the ligands and their tin complexes ...200318365062
cloning and expression of resistance gene analogs (rgas) from wild banana resistant to banana fusarium wilt.wild banana species are essential natural gene pools for banana improvement. in this study, six rgas about 500 bp were obtained from leaves of musa acuminata, a wild banana shown to be resistant to banana fusarium wilt race 4, by pcr amplification with degenerate primers designed according to the conserved nbs motif and serine/threonine kinase domain of plant resistance (r) genes. among these rgas, the deduced amino acids of wnb1 and wnb2 contain nb-arc domain and wnb1 can be translated into pol ...200718349511
chemistry and weak antimicrobial activities of phomopsins produced by mangrove endophytic fungus phomopsis sp. zsu-h76.three metabolites named phomopsin a (1), b (2) and c (3), together with two known compounds cytosporone b (4) and c (5), were isolated from the mangrove endophytic fungus, phomopsis sp. zsu-h76 obtained from the south china sea. their structures were elucidated by spectroscopic methods, mainly by 1d and 2d nmr spectroscopic techniques. the medium-sized cyclic phenol ether based on 1 or 2 is rare in natural products. in bioassays, compounds 1, 2, and 3 had no significant antibiotic activities, bu ...200818343465
the plant defensin, nad1, enters the cytoplasm of fusarium oxysporum hyphae.the plant defensin, nad1, from the flowers of nicotiana alata displays potent antifungal activity against a variety of agronomically important filamentous fungi including fusarium oxysporum f. sp. vasinfectum (fov). to understand the mechanism of this antifungal activity, the effect of nad1 on fov fungal membranes and the location of nad1 in treated hyphae was examined using various fluorescence techniques. nad1 permeabilized fungal plasma membranes via the formation of an aperture with an inter ...200818339623
recent developments in the molecular discrimination of formae speciales of fusarium oxysporum.rapid and reliable detection and identification of potential plant pathogens is required for taking appropriate and timely disease management measures. for many microbial species of which all strains generally are plant pathogens on a known host range, this has become quite straightforward. however, for some fungal species this is quite a challenge. one of these is fusarium oxysporum schlechtend:fr., which, as a species, has a very broad host range, while individual strains are usually highly ho ...200818335459
[screening, identification, and antagonism assessment, of dominant bacteria in ageratina adenophora sprengel rhizosphere soil].by using isolation and culture method, 25 strains of dominant bacteria in ageratina adenophora rhizosphere soil were isolated and identified, of which, 8 strains were assessed for their antagonistic activity. the results showed that bacillus and pseudomonas were highly abundant in a. adenophora rhizosphere soil, of which, b. subtilis and b. megaterium were most abundant and occupied 55.6% of the total identified bacteria. these dominant bacteria had different level antagonistic activity to fusar ...200718333457
clavatol and patulin formation as the antagonistic principle of aspergillus clavatonanicus, an endophytic fungus of taxus mairei.many endophytic fungi are known to protect plants from plant pathogens, but the antagonistic mechanism has rarely been revealed. in this study, we wished to learn whether an endophytic aspergillus sp., isolated from taxus mairei, would indeed produce bioactive components, and if so whether (a) they would antagonize plant pathogenic fungi; and (b) whether this aspergillus sp. would produce the compound also under conditions of confrontation with these fungi. the endophytic fungal strain from t. m ...200818317751
the presence of a virulence locus discriminates fusarium oxysporum isolates causing tomato wilt from other isolates.fusarium oxysporum is an asexual fungus that inhabits soils throughout the world. as a species, f. oxysporum can infect a very broad range of plants and cause wilt or root rot disease. single isolates of f. oxysporum, however, usually infect one or a few plant species only. they have therefore been grouped into formae speciales (f.sp.) based on host specificity. isolates able to cause tomato wilt (f.sp. lycopersici) do not have a single common ancestor within the f. oxysporum species complex. he ...200818312397
arabidopsis defense response against fusarium oxysporum.the plant fungal pathogen fusarium oxysporum (fox) is the causal agent of root rot or wilt diseases in several plant species, including crops such as tomato (solanum lycopersicum), banana (musa sapientum) and asparagus (asparagus officinalis). colonization of plants by fox leads to the necrosis of the infected tissues, a subsequent collapse of vascular vessels and decay of the plant. plant resistance to fox appears to be monogenic or oligogenic depending on the host. perception of fox by plants ...200818289920
diversity in beauvericin and enniatins h, i, and mk1688 by fusarium oxysporum isolated from potato.beauvericins and enniatins are cyclohexadepsipeptide mycotoxins that exhibit phytotoxicity and insecticidal activities. in the present study, the production of beauvericin and newly found enniatins (h, i, and mk1688) was characterized in 28 fusarium strains isolated from potato samples in korea. the predominant fusarium species in potato was f. oxysporum (53.6%). fifteen strains of f. oxysporum and two strains of other fusarium species produced beauvericin (at concentrations from 3.1 to 743.2 mi ...200818279990
accumulation of cell wall-bound phenolic metabolites and their upliftment in hairy root cultures of tomato (lycopersicon esculentum mill.).alkaline hydrolysis of cell wall material of tomato hairy roots yielded ferulic acid as the major phenolic compound. other phenolics were 4-hydroxybenzoic acid, vanillic acid, 4-hydroxybenzaldehyde, vanillin and 4-coumaric acid. the content of phenolics was much higher at the early stage of hairy root growth. the ferulic acid content decreased up to 30 days and then sharply increased to 360 microg/g at 60 days of growth. elicitation of hairy root cultures with fusarium mat extract (fme) increase ...200818273552
virulence analysis and oligonucleotide fingerprinting to detect diversity among indian isolates of fusarium oxysporum f. sp. ciceris causing chickpea wilt.virulence analysis of 64 isolates of fusarium oxysporum f. sp. ciceris causing chickpea wilt collected from major chickpea growing states of india on 14 varieties, including 10 international differentials revealed that the isolates from each state were highly variable. based on the reactions on international differentials, more than one race was found to be prevalent in every state. majority of the isolates were not matched with the race specific reactions. therefore, some of the cultivars, name ...200818266072
antifungal susceptibility profile of clinical fusarium spp. isolates identified by molecular methods.to analyse the susceptibility pattern of a collection of fusarium clinical isolates.200818263569
denitrification by the fungus fusarium oxysporum involves nadh-nitrate reductase.fusarium oxysporum jcm11502 expresses a denitrifying (nitrate (no(3)(-))-respiring) mechanism and can thrive under oxygen (o(2)) limitation. the fungus reduces no(3)(-) to nitrite at the initial step of denitrification. in this study, we cloned the gene coding nadh-no(3)(-) reductase (nadh-nar) (niad) from f. oxysporum jcm11502. the niad gene complemented the defective no(3)(-) assimilation by mutant strain m10, indicating that the fungus reduced no(3)(-) through nadh-nar activity and assimilate ...200818256499
characterization of antimicrobial compounds produced by pseudomonas aurantiaca s-1.pseudomonas aurantiaca s-1 can serve as a natural source of pesticides towards phytopathogens like fusarium oxysporum p1 and pseudomonas syringae pv. glycinea bim b-280. the largest pool of produced antimicrobial compounds was found in four days-old spent culture supernatant. at least two groups of bioactive substances were identified, one responsible for the antibacterial activity and the other for the antifungal activity. the fraction with strong antibacterial activity had the molecular mass 2 ...200718254494
rho1 has distinct functions in morphogenesis, cell wall biosynthesis and virulence of fusarium oxysporum.rho-type gtpases regulate polarized growth in yeast by reorganization of the actin cytoskeleton and through signalling pathways that control the expression of cell wall biosynthetic genes. we report the cloning and functional analysis of rho1 from fusarium oxysporum, a soilborne fungal pathogen causing vascular wilt on plants and opportunistic infections in humans. f. oxysporum strains carrying either a deltarho1 loss-of-function mutation or a rho1(g14v) gain-of-function allele were viable, but ...200818248628
fungal root endophytes from natural vegetation in mediterranean environments with special reference to fusarium spp.surveys (in 2002 and 2003) were performed for fungal endophytes in roots of 24 plant species growing at 12 sites (coastal and inland soils, both sandy soils and salt marshes) under either water or salt stress in the alicante province (southeast spain). all plant species examined were colonized by endophytic fungi. a total of 1830 fungal isolates were obtained and identified by morphological and molecular [internal transcribed spacer (its) and translation elongation factor-1alpha gene region (tef ...200818248439
degradation of the fungicide carbendazim in aqueous solutions with uv/tio(2) process: optimization, kinetics and toxicity studies.an attempt was made to investigate the potential of uv-photocatalytic process in the presence of tio(2) particles for the degradation of carbendazim (c(9)h(9)n(3)o(2)), a fungicide with a high worldwide consumption but considered as a "priority hazard substance" by the water framework directive of the european commission (wfdec). a circulating upflow photo-reactor was employed and the influence of catalyst concentration, ph and temperature were investigated. the results showed that degradation o ...200818243543
statistical optimization of growth medium for the production of the entomopathogenic and phytotoxic cyclic depsipeptide beauvericin from fusarium oxysporum kfcc 11363p.the production of the entomopathogenic and phytotoxic cyclic depsipeptide beauvericin (bea) was studied in submerged cultures of fusarium oxysporum kfcc 11363p isolated in korea. the influences of various factors on mycelia growth and bea production were examined in both complete and chemically defined culture media. the mycelia growth and bea production were highest in fusarium defined medium. the optimal carbon and nitrogen sources for maximizing bea production were glucose and nano3, respecti ...200818239431
naphthoquinone spiroketal with allelochemical activity from the newly discovered endophytic fungus edenia gomezpompae.chemical investigation of the mycelium of edenia gomezpompae, a newly discovered endophytic fungus isolated from the leaves of callicarpa acuminata (verbenaceae) collected from the ecological reserve el eden, quintana roo, mexico, resulted in the isolation of four naphthoquinone spiroketals, including three new compounds and palmarumycin cp2 (4). we elucidated the structures of the metabolites by extensive nmr spectroscopy studies, including dept, cosy, noesy, hsqc, hmbc, and chiroptical methods ...200818234248
lysobacter capsici sp. nov., with antimicrobial activity, isolated from the rhizosphere of pepper, and emended description of the genus lysobacter.the taxonomic position of a novel bacterial strain, yc5194(t), with antimicrobial activity, isolated from the rhizosphere of pepper in jinju, south korea, was studied using a polyphasic approach. cells of the strain were gram-negative, rod-shaped, facultative anaerobes. it grew at a temperature of 15-37 degrees c (optimum 28 degrees c). growth of the strain occurred between ph 5.5 and 8.5, with an optimum of ph 7.0-7.5. the strain inhibited mycelial growth of pythium ultimum, colletotrichum gloe ...200818218936
isolation and analysis of bacteria associated with spores of gigaspora margarita.the aim of this work was to observe bacteria associated with the spores of gigaspora margarita, an arbuscular mycorrhizal fungus (amf).200818217929
molecular cloning and characterization of ghnpr1, a gene implicated in pathogen responses from cotton (gossypium hirsutum l.).a novel gene, designated as ghnpr1 (gossypium hirsutum non-expressor of pathogenesis-related genes 1), was isolated from g. hirsutum (cotton) by rt-pcr (reverse transcription-pcr) and race (rapid amplification of cdna ends). the full-length cdna was 2108 bp long and had an orf (open reading frame) that putatively encoded a polypeptide of 592 amino acids, with a predicted molecular mass of 66 kda. comparison of this protein sequence with that of arabidopsis thaliana, brassica juncea and nicotiana ...200818215146
cinnamic acid inhibits growth but stimulates production of pathogenesis factors by in vitro cultures of fusarium oxysporum f.sp. niveum.long-term monoculture of watermelon leads to frequent occurrence of watermelon fusarium wilt caused by fusarium oxysporum f.sp. niveum (fon). some allelochemicals contained in watermelon root exudates and decaying residues are possibly responsible for promoting the wilt disease. the purpose of this study was to evaluate the allelopathic effect of artificially applied cinnamic acid on fon. results demonstrated that hyphal growth of fon was strongly inhibited by cinnamic acid. at the highest conce ...200818211014
antifungal activity of essential oil and its constituents from calocedrus macrolepis var. formosana florin leaf against plant pathogenic fungi.resistance to conventional fungicides causes the poor disease control of agriculture. natural products from plants have great potential as novel fungicide sources for controlling pathogenic fungi. in this study antipathogenic activity of the leaf essential oil and its constituents from calocedrus macrolepis var. formosana florin were evaluated in vitro against six plant pathogenic fungi. chemical analysis of leaf oil by gc/ms allowed identification of alpha-pinene (44.2%), limonene (21.6%), beta ...200818206367
endophytic actinobacteria induce defense pathways in arabidopsis thaliana.endophytic actinobacteria, isolated from healthy wheat tissue, which are capable of suppressing a number wheat fungal pathogens both in vitro and in planta, were investigated for the ability to activate key genes in the systemic acquired resistance (sar) or the jasmonate/ethylene (ja/et) pathways in arabidopsis thaliana. inoculation of a. thaliana (col-0) with selected endophytic strains induced a low level of sar and ja/et gene expression, measured using quantitative polymerase chain reaction. ...200818184065
interactions between glyphosate and autochthonous soil fungi surviving in aqueous solution of glyphosate.the survival of autochthonous fungi in soil treated with 1mm aqueous solution of glyphosate was investigated. significant differences in the total number of fungi in the studied objects were observed, and additionally significant qualitative changes were encountered. the dominating group of fungi belonged to genus fusarium: fusarium solani h30, fusarium solani h50 and fusarium oxysporum h80. interactions between the isolated strains of fungi and varying concentrations of glyphosate were determin ...200818177917
mycobiota of the date palm phylloplane: description and interactions.we have analysed the mycobiota of date palm (phoenix dactylifera, l.) leaves using several techniques. profusely sporulating fungi (penicillium spp. and cladosporium spp.) developed when plating leaf fragments and leaf washings. fusarium oxysporum, was particularly abundant in leaves infested with the red scale insect phoenicococcus marlatti cockerell, 1899, but an undescribed lecanicillium cf. psalliotae was also found. dual and overlay cultures showed interactions between palm pathogens, entom ...200718095764
pcr-based specific detection of ralstonia solanacearum by amplification of cytochrome c1 signal peptide sequences.a polymerase chain reaction (pcr)-based method was developed to detect the dna of ralstonia solanacearum, the causal agent of bacterial wilt in various crop plants. one pair of primers (ralsf and ralsr), designed using cytochrome c1 signal peptide sequences specific to r. solanacearum, produced a pcr product of 932 bp from 13 isolates of r. solanacearum from several countries. the primer specificity was then tested using dna from 21 isolates of ralstonia, pseudomonas, burkholderia, xanthomonas, ...200718092459
synthesis of acyl thiourea derivatives of chitosan and their antimicrobial activities in vitro.three different acyl thiourea derivatives of chitosan (cs) were synthesized and their structures were characterized by ft-ir spectroscopy and elemental analysis. the antimicrobial behaviors of cs and its derivatives against four species of bacteria (escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, and sarcina) and four crop-threatening pathogenic fungi (alternaria solani, fusarium oxysporum f. sp. vasinfectum, colletotrichum gloeosporioides (penz.) saec, and phyllisticta zingiber ...200818083151
anti-lipopolysaccharide factor in litopenaeus vannamei (lvalf): a broad spectrum antimicrobial peptide essential for shrimp immunity against bacterial and fungal infection.antimicrobial peptides are an essential component of the innate immune system of most organisms. expressed sequence tag analysis from various shrimp (litopenaeus vannamei) tissues revealed transcripts corresponding to two distinct sequences (lvalf1 and lvalf2) with strong sequence similarity to anti-lipopolysaccharide factor (alf), an antimicrobial peptide originally isolated from the horseshoe crab limulus polyphemus. full-length clones contained a 528bp transcript with a predicted open reading ...200818078996
inhibitory activity against plant pathogenic fungi of extracts from myoporum bontioides a. gray and indentification of active ingredients.in order to understand the bioactivity of myoporum bontioides a. gray against plant pathogens and determine its active ingredients, the inhibitory activities of methanol extracts from m. bontioides against fusarium oxysporum f. sp. niveum (e. f. smith) snyder & hansen, pestalotia mangiferae p. henn., thielaviopsis paradoxa (de seynes) v. hohnel, colletotrichum musae (berk. & m. a. curtis) v. arx, alternaria alternata (fr.) keissler, mycosphaerella sentina (fr.) schroter and sphaceloma fawcettii ...200818069657
diagnostic value of dna and (1-->3)- beta-d-glucan detection in serum and bronchoalveolar lavage of mice experimentally infected with fusarium oxysporum.a sensitive and highly specific nested pcr (npcr) protocol was developed for the specific detection of fusarium oxysporum dna in clinical specimens. the diagnostic value of f. oxysporum-specific dna and (1-->3)-beta-d-glucan (bdg) detection was subsequently evaluated in serum and bronchoalveolar lavage (bal) specimens of mice infected intravenously with f. oxysporum conidia. mice were sacrificed in groups of six daily up to day 8 and then on days 11 and 14. the f. oxysporum-specific dna and bdg ...200818065665
biological activity of phenylpropionic acid isolated from a terrestrial streptomycetes.the strain anu 6277 was isolated from laterite soil and identified as streptomyces sp. closely related to streptomyces albidoflavus cluster by 16s rrna analysis. the cultural, morphological and physiological characters of the strain were recorded. the strain exhibited resistance to chloramphenicol, penicillin and streptomycin. it had the ability to produce enzymes such as amylase and chitinase. a bioactive compound was isolated from the strain at stationary phase of culture and identified as 3-p ...200718062653
production of biosurfactants and antibiotics by fluorescent pseudomonads isolated from a closed hydroponic system equipped with a slow filter.the presence of antibiotic- and biosurfactant-producing strains of fluorescent pseudomonads in a closed hydroponic system equipped with a slow filter was investigated. a total of 271 strains of pseudomonads were isolated before the filter, from the filter skin and from the effluent. production of biosurfactants was determined using the drop-collapse method. the ability of the strains to inhibit the growth of the plant pathogens pythium ultimum, phytophthora cryptogea and fusarium oxysporum was d ...200818058254
screenhouse and field persistence of nonpathogenic endophytic fusarium oxysporum in musa tissue culture plants.two major biotic constraints to highland cooking banana (musa spp., genome group aaa-ea) production in uganda are the banana weevil cosmopolites sordidus and the burrowing nematode radopholus similis. endophytic fusarium oxysporum strains inoculated into tissue culture banana plantlets have shown control of the banana weevil and the nematode. we conducted screenhouse and field experiments to investigate persistence in the roots and rhizome of two endophytic fusarium oxysporum strains, v2w2 and i ...200818058162
antifungal activity of some diaryl ethers.several diaryl ethers were synthesized and tested in vitro against seven phytopathogenic fungi, namely fusarium graminearum, alternaria alternate, helminthosporium sorokinianum, pyricularia oryzae, fusarium oxysporum f. sp. vasinfectum, fusarium oxysporum f. sp. cucumarinum and alternaria brassicae. compared to a commercial agricultural fungicide, hymexazol, especially compounds a, b, e, g and k were found to be more effective at 50 mug/ml against f. graminearum, f. oxysporum f. sp. vasinfectum ...200718057754
in vitro interactions of antifungal agents against clinical isolates of fusarium spp.the in vitro activities of amphotericin b (amb), itraconazole (itc), voriconazole (vcz) and terbinafine (tbf) alone and in the combinations amb+vcz, tbf+itc and tbf+vcz were evaluated against 29 clinical isolates of fusarium spp. (15 fusarium solani, 7 fusarium oxysporum, 2 fusarium decemcellulare, 2 fusarium dimerum and 3 other fusarium spp.). minimum inhibitory concentrations were determined using the method of the clinical and laboratory standards institute and the interaction activity was ca ...200818054469
plant growth-promoting potential of endophytic bacteria isolated from roots of coastal sand dune plants.endophytic bacteria associated with the roots of coastal sand dune plants were isolated, taxonomically characterized, and tested for their plant growth-promoting activities. ninety-one endophytic bacterial isolates were collected and assigned to 17 different genera of 6 major bacterial phyla based on partial 16s rdna sequence analyses. gammaproteobacteria represented the majority of the isolates (65.9%), and members of pseudomonas constituted 49.5% of the total isolates. when testing for antagon ...200718051606
escherichia coli can produce recombinant chitinase in the soil to control the pathogenesis by fusarium oxysporum without colonization.fusarium wilt of cucumbers was effectively controlled by escherichia coli expressing an endochitinase gene (chia), and the rate was as effective (60.0%) as the wildtype strain s. proteamaculans 3095 (55.0%) where the gene was cloned. however, live cells of soil inoculated e. coli host harboring the chia gene did not proliferate but declined 100-fold from 108 cfu during the first week and showed less than 10 cells after day 14, suggesting that e. coli was able to express and produce the chitinase ...200718050952
role of ethylene in the protection of tomato plants against soil-borne fungal pathogens conferred by an endophytic fusarium solani strain.an endophytic fungal isolate (fs-k), identified as a fusarium solani strain, was obtained from root tissues of tomato plants grown on a compost which suppressed soil and foliar pathogens. strain fs-k was able to colonize root tissues and subsequently protect plants against the root pathogen fusarium oxysporum f.sp. radicis-lycopersici (forl), and elicit induced systemic resistance against the tomato foliar pathogen septoria lycopersici. interestingly, attenuated expression of certain pathogenesi ...200718048373
the in vitro effect of gossypol and its interaction with salts on conidial germination and viability of fusarium oxysporum sp. vasinfectum isolates.to assess the effect of different concentrations of gossypol (0, 2, 4, 10 and 20 mg l(-1)) in combination with nacl and na(2)so(4) (20 ms cm(-1)) on the conidial germination and viability of fusarium oxysporum f.sp. vasinfectum (fov).200718045422
isolation and characterization of a trypsin-chymotrypsin inhibitor from the seeds of green lentil (lens culinaris).a bowman-birk type trypsin-chymotrypsin inhibitor was isolated from seeds of the legume green lentil (lens culinaris) by means of affinity chromatography on affi-gel blue gel, ion exchange chromatography on q-sepharose, ion exchange chromatography by fast protein liquid chromatography (fplc) on mono q and mono s, and gel filtration by fplc on superdex 75. the trypsin-chymotrypsin inhibitor was bound on the first three types of chromatographic media. it appeared as a single 16-kda peak in gel fil ...200718045226
differences among strains of the fusarium oxysporum-f. solani complexes in their penetration of hydrogel contact lenses and subsequent susceptibility to multipurpose contact lens disinfection solutions.to examine in vitro conditions for attachment and penetration of silicone hydrogel (sh) lenses by clinical isolates of the fusarium oxysporum-f. solani complexes and the relative susceptibilities of the fusaria in the lens matrices to multipurpose contact lens solutions (mpss).200718043184
the drought response of theobroma cacao (cacao) and the regulation of genes involved in polyamine biosynthesis by drought and other stresses.drought can negatively impact pod production despite the fact that cacao production usually occurs in tropical areas having high rainfall. polyamines (pas) have been associated with the response of plants to drought in addition to their roles in responses to many other stresses. the constitutive and drought inducible expression patterns of genes encoding enzymes involved in pa biosynthesis were determined: an ornithine decarboxylase (tcodc), an arginine decarboxylase (tcadc), an s-adenosylmethio ...200818042394
vegetative hyphal fusion is not essential for plant infection by fusarium oxysporum.vegetative hyphal fusion (vhf) is a ubiquitous phenomenon in filamentous fungi whose biological role is poorly understood. in neurospora crassa, the mitogen-activated protein kinase (mapk) mak-2 and the ww domain protein so are required for efficient vhf. a mapk orthologous to mak-2, fmk1, was previously shown to be essential for root penetration and pathogenicity of the vascular wilt fungus fusarium oxysporum. here we took a genetic approach to test two hypotheses, that (i) vhf and plant infect ...200818039941
optimization of spore and antifungal lipopeptide production during the solid-state fermentation of bacillus subtilis.bacillus subtilis strain trigocor 1448 was grown on wheat middlings in 0.5-l solid-state fermentation (ssf) bioreactors for the production of an antifungal biological control agent. total antifungal activity was quantified using a 96-well microplate bioassay against the plant pathogen fusarium oxysporum f. sp. melonis. the experimental design for process optimization consisted of a 2(6-1) fractional factorial design followed by a central composite face-centered design. initial ssf parameters inc ...200718025597
chsvb, a class vii chitin synthase involved in septation, is critical for pathogenicity in fusarium oxysporum.a new myosin motor-like chitin synthase gene, chsvb, has been identified in the vascular wilt fungus fusarium oxysporum f. sp. lycopersici. phylogenetic analysis of the deduced amino acid sequence of the chsvb chitin synthase 2 domain (cs2) revealed that chsvb belongs to class vii chitin synthases. the chsvb myosin motor-like domain (mmd) is shorter than the mmd of class v chitin synthases and does not contain typical atp-binding motifs. targeted disrupted single (deltachsvb) and double (deltach ...200817993572
identification and functional analysis of the fusaricidin biosynthetic gene of paenibacillus polymyxa e681.fusaricidin, a peptide antibiotic consisting of six amino acids, has been identified as a potential antifungal agent from paenibacillus polymyxa. here, we report the complete sequence of the fusaricidin synthetase gene (fusa) identified from the genome sequence of a rhizobacterium, p. polymyxa e681. the gene encodes a polypeptide consisting of six modules in a single open-reading frame. interestingly, module six of fusa does not contain an epimerization domain, which suggests that the sixth amin ...200817980146
chitinase-like proteins with antifungal activity from emperor banana fruits.two 30-kda proteins with n-terminal sequence homology to chitinases have been isolated from fruits of the emperor banana by using a protocol that involved (nh(4))(2)so(4) precipitation, affinity chromatography on affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatography (fplc) on mono s and gel filtration by fplc on superdex 75. the proteins were adsorbed on affi-gel blue gel and mono s. they both inhibited mycelial growth in fusarium oxysporum but not in mycosphaerell ...200717979827
hypotin, a novel antipathogenic and antiproliferative protein from peanuts with a sequence similar to those of chitinase precursors.a protein designated hypotin, with both antifungal and antibacterial activity, was isolated from peanut (arachis hypogaea) seeds. the isolation procedure included extraction, ammonium sulfate precipitation, affinity chromatography on affi-gel blue gel, ion chromatography, and gel filtration. the protein exhibited a molecular mass of 30.4 kda in sds-polyacrylamide gel electrophoresis under both reducing and nonreducing conditions, indicating that it is a monomeric protein. its n-terminal sequence ...200717979231
the fusarium oxysporum sti35 gene functions in thiamine biosynthesis and oxidative stress response.the sti35 gene of the vascular wilt fungus fusarium oxysporum was originally identified based on induced expression under stress conditions. in this study, the transcriptional regulation and biological function of sti35 were examined in the tomato pathogen f. oxysporum f.sp. lycopersici. expression of sti35 was repressed by thiamine and induced by high temperatures. sti35 transcripts were detected both during early and late stages of infection of tomato plants by f. oxysporum. heterologous expre ...200817964830
isolation and characterization of antagonistic bacillus subtilis strains from the avocado rhizoplane displaying biocontrol activity.this study was undertaken to isolate bacillus subtilis strains with biological activity against soil-borne phytopathogenic fungi from the avocado rhizoplane.200717953605
serological detection and immunogold localization of cross-reactive antigens shared by camellia sinensis and exobasidium vexans.pathogenicity of exobasidium vexans, causal agent of blister blight of tea, was studied in 30 commercially cultivated tea varieties by analysing the antigenic patterns of host and pathogen using immunological techniques.200717953578
cutinase production by fusarium oxysporum in liquid medium using central composite design.the objective of the present study was to measure the production of cutinase by fusarium oxysporum in the presence of several carbon and nitrogen sources (glycides, fatty acids and oils, and several organic and inorganic nitrogen sources), trying to find a cost-effective substitute for cutin in the culture medium as an inducer of cutinase production. the results were evaluated by the tukey test, and flaxseed oil was found to give the best results as a cutinase inducer. the authors optimized the ...200817938980
antifungal effects of volatile compounds from black zira (bunium persicum) and other spices and herbs.the dish pack method, which measures growth inhibition or promotion effects of volatile compounds on germinating seeds, was applied to measure the antifungal effects of 52 dried samples of spices and herbs against a soil-borne phytopathogenic fungus, fusarium oxysporum. black zira showed the strongest effect, followed by cumin and cardamom. headspace sampling and gas chromatography-mass spectrometry analysis of black zira identified seven volatile compounds, gamma-terpinene, limonene, p-cymene, ...200717932718
anaerobic elemental sulfur reduction by fungus fusarium oxysporum.reduction of inorganic sulfur compounds by the fungus fusarium oxysporum was examined. when transferred from a normoxic to an anoxic environment, f. oxysporum reduced elemental sulfur to hydrogen sulfide (h2s). this reaction accompanied fungal growth and oxidation of the carbon source (ethanol) to acetate. over 2-fold more of h2s than of acetate was produced, which is the theoretical correlation for the oxidation of ethanol to acetate. nadh-dependent sulfur reductase (sr) activity was detected i ...200717928705
effect of chitosan on hyphal growth and spore germination of plant pathogenic and biocontrol fungi.to investigate the toxic effect of chitosan on important root pathogenic and biocontrol fungi (nematophagous, entomopathogenic and mycoparasitic).200817927761
bikaverin and fusaric acid from fusarium oxysporum show antioomycete activity against phytophthora infestans.to isolate and identify antioomycete substances from fusarium oxysporum ef119 against phytophthora infestans and to investigate their antimicrobial activities against various plant pathogenic bacteria, oomycetes and true fungi.200817927749
reclamation of chitinous materials by bromelain for the preparation of antitumor and antifungal materials.the main purpose of this research was to investigate the antitumor and antimicrobial activities of the chitooligosaccharides containing hydrolyzates obtained from the hydrolysis of chitinous materials (such as chitin, chitosan, and squid pen) by bromelain. the optimum preparations were gained in the hydrolysates of squid pen powder hydrolyzed at ph 5, 37 degrees c for 2 days by 0.1% bromelain. the hydrolysates had an 80% inhibitory activity on phyto-pathogenic mold fusarium oxysporum. chitooligo ...200817920262
fusarium oxysporum-induced oxidative stress and antioxidative defenses of yellow lupine embryo axes with different sugar levels.this study was designed to investigate whether and to what extent oxidative stress is induced in embryo axes of lupinus luteus l. cv. polo inoculated with a necrotrophic fungus, fusarium oxysporum and cultured on heller medium for 96h. four variants were compared: inoculated embryo axes cultured with 60mm sucrose (+si) or without it (-si), and non-inoculated embryo axes cultured with 60mm sucrose (+sn) or without it (-sn). after inoculation, an accumulation of stable free radicals and mn2+ ions ...200817913293
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