Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| antibacterial properties of imipenem with special reference to the activity against methicillin-resistant staphylococci, cefotaxime-resistant enterobacteriaceae and pseudomonas aeruginosa. | imipenem was examined with standardized agar dilution procedures against a wide range of bacteria. geometric mean mics against the genera escherichia, klebsiella, enterobacter, citrobacter and serratia were 0.1-0.4 mg/l, and proteus and providencia spp. were inhibited by 0.25-4 mg/l. acinetobacter calcoaceticus var. anitratum strains were inhibited by concentrations ranging from 0.12-0.5 mg/l. methicillin-susceptible staphylococci were highly susceptible to the drug (mics: less than or equal to ... | 1986 | 3102452 |
| determination of endotoxins on hypodermic needles by means of a chromogenic limulus amoebocyte lysate assay. development of a test model. | a test model was developed in order to describe the determination of endotoxins on hypodermic needles in a reliable and reproducible manner. as in all in-vitro experiments one has to be very careful about extrapolating data to in-vivo situations. however by choosing a hydrophilic (salmonella typhimurium atcc 14028) and a hydrophobic bacterium (acinetobacter calcoaceticus var. anitratus rr 8212/113), one could hope to obtain a quite representative idea about the extraction of contaminating gram-n ... | 1986 | 3101323 |
| enhanced emulsan production in mutants of acinetobacter calcoaceticus rag-1 selected for resistance to cetyltrimethylammonium bromide. | mutants of acinetobacter calcoaceticus rag-1 that produced elevated levels of the polymeric bioemulsifier emulsan were isolated on the basis of their resistance to the cationic surfactant cetyltrimethylammonium bromide (ctab). such mutants showed maximum enhancement in both overall yield and specific productivity of some two- to threefold over that of the wild type. in addition, the effect was also observed in a resting cell system in the presence of chloramphenicol, indicating that the mutation ... | 1986 | 3089157 |
| in vitro activity of aztreonam against gram negative bacteria from clinical specimens and its comparison with other commonly used antibiotics. | a total of 755 gram negative bacteria isolated from clinical specimens were tested against aztreonam by the disc agar diffusion test. the strains of bacteria used in this study consisted of escherichia coli (314), enterobacter aerogenes (30), e. agglomerans (7), e. cloacae, (39), citrobacter diversus (9), c. freundii (13), hafnia alvei (3), acinetobacter calcoaceticus (10), klebsiella oxytoca (6), k. ozaenae (5), k. pneumoniae (107), morganella morganii (3), moraxella sp. (10), pasteurella multo ... | 1986 | 3088346 |
| microplate technique to determine hemolytic activity for routine typing of listeria strains. | because the hemolysis produced by listeria monocytogenes and listeria seeligeri on blood agar is frequently difficult to interpret, we developed a microplate technique for the routine determination of hemolytic activity with erythrocyte suspensions. this microtechnique is a simple and reliable test for distinguishing clearly between hemolytic and nonhemolytic strains and could be used instead of the camp (christie-atkins-munch-petersen) test with staphylococcus aureus in the routine typing of li ... | 1986 | 3088037 |
| separation of isoenzymes of citrate synthase and isocitrate dehydrogenase by fast protein liquid chromatography. | fast protein liquid chromatography (fplc) has been shown to be a rapid and effective method of separating isoenzymes of citrate synthase and isocitrate dehydrogenase in extracts of pseudomonas aeruginosa and acinetobacter calcoaceticus. the advantages of fplc over conventional methods of fractionation are discussed and it is suggested that this may be a valuable and more general technique for isoenzyme resolution. | 1986 | 3081366 |
| antibiogram, biotyping and plasmid profile analysis of acinetobacter calcoaceticus var. anitratus. | antibiogram, biotyping, and plasmid profile analysis were studied on 115 isolates (40 nosocomial and 75 community strains) of acinetobacter calcoaceticus var. anitratus isolated from clinical specimens. by disk diffusion method, we found 14 antimicrobial susceptibility patterns. among them, the gentamicin-resistant patterns were recovered more frequently from nosocomial origin than from community-acquired origin. fifteen patterns of biocodes were found by using gni card of automicrobic system (v ... | 1988 | 3076859 |
| cloning and expression of pca genes from pseudomonas putida in escherichia coli. | beta-ketoadipate elicits expression of five structural pca genes encoding enzymes that catalyse consecutive reactions in the utilization of protocatechuate by pseudomonas putida. three derivatives of p. putida prs2000 were obtained, each carrying a single copy of tn5 dna inserted into a separate region of the genome and preventing expression of different sets of pca genes. selection of tn5 in or near the pca genes in these derivatives was used to clone four structural pca genes and to enable the ... | 1988 | 3076176 |
| comparative in vitro activity of lomefloxacin and other antimicrobials against 597 microorganisms causing bacteremia. | the in vitro activity of lomefloxacin, a new fluorinated quinolone antimicrobial, was compared to that of enoxacin, norfloxacin, ofloxacin, imipenem, cefotaxime, ceftazidime, and tobramycin against 597 microorganisms isolated from bacteremic patients at a university hospital. overall, lomefloxacin had activity similar to that of enoxacin and norfloxacin but less than that of ofloxacin. lomefloxacin had excellent activity against members of the family enterobacteriaceae and haemophilus influenzae ... | 1988 | 3071448 |
| acinetobacter calcoaceticus bacteremia 1984-1987. | 1988 | 3068198 | |
| flow cytometric screening and isolation of escherichia coli clones which express surface antigens of the oil-degrading microorganism acinetobacter calcoaceticus rag-1. | flow cytometry (fcm) in conjunction with immunocytochemical-labeling was used to analyze and screen a population of escherichia coli clones containing a genomic library from the oil-degrading microorganism acinetobacter calcoaceticus rag-1 surface antigens. reconstruction experiments using mixed populations indicated that rag-1 cells could be clearly distinguished at a ratio of one rag-1 cell to 500 escherichia coli cells. using this technique two clones, wm143 and wm191, were isolated and shown ... | 1988 | 3061370 |
| benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase ii from acinetobacter calcoaceticus. substrate specificities and inhibition studies. | the apparent km and maximum velocity values of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase ii from acinetobacter calcoaceticus were determined for a range of alcohols and aldehydes and the corresponding turnover numbers and specificity constants were calculated. benzyl alcohol was the most effective alcohol substrate for benzyl alcohol dehydrogenase. perillyl alcohol was the second most effective substrate, and was the only non-aromatic alcohol oxidized. the other substrates of b ... | 1988 | 3060114 |
| comparison of mandelate dehydrogenases from various strains of acinetobacter calcoaceticus: similarity of natural and 'evolved' forms. | in previous work it had been shown that acinetobacter calcoaceticus wild-type strain ncib 8250 had only an l-mandelate deydrogenase but it could give rise to mutants that contained an evolved d-mandelate dehydrogenase; conversely, wild-type strain ebf 65/65 had only a d-mandelate dehydrogenase but gave rise to mutants that possessed an evolved l-mandelate dehydrogenase. several other wild-type strains of a. calcoaceticus have now been shown to grow on both enantiomers of mandelate. in every case ... | 1988 | 3053984 |
| a belgian multicentre in-vitro study of ofloxacin. | in a large multicentre study, the susceptibility of 2171 bacterial isolates to ofloxacin and other antibiotics has been examined by the kirby-bauer method. susceptibility to ofloxacin (breakpoint less than or equal to 2 mg/l) was observed in 99.2% of 1104 strains enterobacteriaceae, 88.9% of 217 pseudomonas aeruginosa, 93.6% of 31 other pseudomonas spp., 96.3% of 27 acinetobacter calcoaceticus, 99.2% of 118 haemophilus influenzae, 97.4% of 423 staphylococci, 79.4% of 107 streptococcus faecalis a ... | 1988 | 3053578 |
| chorismate mutase:prephenate dehydratase from acinetobacter calcoaceticus. purification, properties and immunological cross-reactivity. | the bifunctional p protein (chorismate mutase: prephenate dehydratase) from acinetobacter calcoaceticus has been purified. it was homogeneous in polyacrylamide gels and was more than 95% pure on the basis of the immunostaining of purified p protein with the antibodies raised against the p protein. the native enzyme is a homodimer (mr = 91,000) composed of 45-kda subunits. a twofold increase in the native molecular mass of the p protein occurred in the presence of l-phenylalanine (inhibitor of bo ... | 1988 | 3046943 |
| bacterial nad(p)-independent quinate dehydrogenase is a quinoprotein. | acinetobacter calcoaceticus lmd 79.41 produced significant amounts of pyrrolo-quinoline quinone (pqq) in its culture medium when grown on quinic acid or shikimic acid. studies with lmd 79.41 and pqq- -mutants of this strain demonstrated that this organism contains an nad(p)-independent quinate dehydrogenase (qdh) (ec 1.1.99.-), catalyzing the first degradation step of these compounds, and that the enzyme contains pqq as a cofactor, i.e. is a quinoprotein. synthesis of qdh was induced by protocat ... | 1988 | 3044290 |
| outer membrane permeability of acinetobacter calcoaceticus mediates susceptibility to rat polymorphonuclear leukocyte granule contents. | growth of acinetobacter calcoaceticus on specific alkanes altered the outer membrane permeability of the organism, as indicated by a change in sensitivity to the antibiotic actinomycin d. as the carbon length of the alkane energy source decreased, outer membrane permeability and susceptibility to actinomycin d increased. concomitant with the increase in outer membrane permeability, a. calcoaceticus became more susceptible to the oxygen-independent antimicrobial activity of extracted contents fro ... | 1987 | 3040593 |
| cloning and genetic organization of the pca gene cluster from acinetobacter calcoaceticus. | the beta-ketoadipate pathway of acinetobacter calcoaceticus comprises two parallel metabolic branches. one branch, mediated by six enzymes encoded by the cat genes, converts catechol to succinate and acetyl coenzyme a (acetyl-coa); the other branch, catalyzed by products of the pca genes, converts protocatechuate to succinate and acetyl-coa by six metabolic reactions analogous or identical to those of the catechol sequence. we used the expression plasmid puc18 to construct expression libraries o ... | 1987 | 3036773 |
| sulbactam/ampicillin: in vitro spectrum, potency, and activity in models of acute infection. | more than 90% of community hospital-isolated strains of staphylococcus (including methicillin-resistant isolates), streptococcus, haemophilus, neisseria, branhamella, bacteroides, escherichia coli, klebsiella, enterobacter aerogenes, proteus, and acinetobacter calcoaceticus were inhibited by the sulbactam/ampicillin (1:2) combination at concentrations of 8 micrograms/16 micrograms per ml. the peak serum level from a 15-min infusion of 1 g/2 g of sulbactam/ampicillin is more than seven times this ... | 1986 | 3026001 |
| polymyxin-b inhibition of lps-induced interleukin-1 secretion by human monocytes is dependent upon the lps origin. | polymyxin-b (pmb) is an antibiotic known to inhibit various biological activities induced by lipopolysaccharides (lps). we have investigated the ability of pmb to inhibit lps-induced interleukin-1 (il-1) secretion by human monocytes in vitro. interleukin-1 was assayed by the conventional comitogenic assay using mice thymocytes. our data demonstrate that pmb (1-2 micrograms/assay)-mediated inhibition of lps-induced il-1 secretion depends on the origin of the lps. interleukin-1 secretions induced ... | 1986 | 3023974 |
| the oxidation of glucose by acinetobacter calcoaceticus: interaction of the quinoprotein glucose dehydrogenase with the electron transport chain. | the coupling of the quinoprotein glucose dehydrogenase to the electron transport chain has been investigated in acinetobacter calcoaceticus. no evidence was obtained to support a previous suggestion that the soluble form of the dehydrogenase and the soluble cytochrome b associated with it are involved in the oxidation of glucose. analysis of cytochrome content, and of reduction of cytochromes in membranes by substrates, and of sensitivity to cyanide indicated that glucose, succinate and nadh are ... | 1986 | 3021895 |
| molecular cloning into tn5 and integration in the pseudomonas aeruginosa chromosome: a tool for heterologous gene expression. | the dna primase gene of the promiscuous incp-1 conjugative plasmid rp1, encoding two polypeptides of 118 and 80 kda, was inserted into the transposon tn5 in escherichia coli. the derivative transposon, tn2523, was then transposed to a temperature-sensitive replication mutant of the promiscuous incp-1 conjugative plasmid r68 at permissive temperature and the plasmid transferred to pseudomonas aeruginosa strain pao. the latter strain was then grown at non-permissive temperature to identify transpo ... | 1986 | 3016149 |
| acinetobacter calcoaceticus encoded mutarotase: nucleotide sequence analysis of the gene and characterization of its secretion in escherichia coli. | the nucleotide sequence of the mutarotase gene from acinetobacter calcoaceticus has been determined. it reveals an open reading frame of 381 amino acids. the codon usage of a. calcoaceticus for this gene is similar to e. coli except for the amino acids leu, ala, glu, and arg where major differences exist. this did not interfere drastically with high level expression in e. coli. the regulatory sequences for the initiation of translation are similar to the ones described for e. coli. the n-termina ... | 1986 | 3012466 |
| molecular analysis of an antibiotic resistance plasmid, pav5, and its derivative plasmids in acinetobacter calcoaceticus. | the non-conjugative plasmid pav5 specifies resistance to kanamycin/neomycin (kmr) and tetracycline (tcr). physical evidence is presented to show that pav5 gives rise to two plasmids, pav51 (kmr) and pav52 (tcr), which are formed by deletion of apparently non-overlapping segments of pav5. expression of tcr has been obtained in escherichia coli and is associated with a 1.9 kb hindiii fragment found in pav5 and in pav52. expression of kmr has been obtained in e. coli and is associated with a 1.3 kb ... | 1985 | 3007656 |
| cloning and expression of acinetobacter calcoaceticus catbcde genes in pseudomonas putida and escherichia coli. | this report describes the isolation and preliminary characterization of a 5.0-kilobase-pair (kbp) ecori dna restriction fragment carrying the catbcde genes from acinetobacter calcoaceticus. the respective genes encode enzymes that catalyze four consecutive reactions in the catechol branch of the beta-ketoadipate pathway: catb, muconate lactonizing enzyme (ec 5.5.1.1); catc, muconolactone isomerase (ec 5.3.3.4); catd, beta-ketoadipate enol-lactone hydrolase (ec 3.1.1.24); and cate, beta-ketoadipa ... | 1986 | 3003031 |
| acciii, a new restriction endonuclease from acinetobacter calcoaceticus. | a new site-specific restriction endonuclease, acciii, was isolated from acinetobacter calcoaceticus. acciii recognizes t/ccgga and cleaves at the position shown by the arrow. acciii activity was inhibited by adenine methylation at the overlapping dam methylase recognition sequence. | 1985 | 3001647 |
| vectors for transposon mutagenesis of non-enteric bacteria. | we have constructed a series of transposon delivery vectors derived from prk2013. since prk2013 has a broad host range transfer system and a cole1 replicon, it can be transferred to, but not replicated in, many non-enteric gram-negative bacteria. thus prk2013 provides an effective mechanism for the transient introduction of a transposon. delivery vectors containing tn7 (tmp str), tn10 (tet), tn10 hh104 (tet), or tn5-132 (tet) have been constructed. when transposition in caulobacter crescentus wa ... | 1985 | 2993823 |
| [spontaneous bacterial peritonitis caused by acinetobacter calcoaceticus. a rare nosocomial infection in the cirrhotic patient]. | 1988 | 2974144 | |
| [acinetobacter calcoaceticus var. iwoffi as the cause of abscess formation in han:nmri nude mice]. | 1988 | 2973310 | |
| comparative antimicrobial activity of enoxacin, ciprofloxacin, amifloxacin, norfloxacin and ofloxacin against 177 bacterial isolates. | the in-vitro antimicrobial activity of five new quinolones, enoxacin (ci-919, at-2266), ciprofloxacin (bay o 9867), amifloxacin (win 49375) norfloxacin (mk 0366), and ofloxacin (orf 18489), was compared against 104 strains of enterobacteriaceae, 51 pseudomonas species, 7 acinetobacter calcoaceticus var. anitratus, and 15 enterococci. in general the quinolones tested were active against most bacterial strains. ciprofloxacin was the most active with mic90 for all genera tested ranging from 0.03 to ... | 1986 | 2941401 |
| ciprofloxacin therapy of infections caused by pseudomonas aeruginosa and other resistant bacteria. | ciprofloxacin was administered orally to 48 patients with 24 pseudomonas aeruginosa infections and 13 other infections caused by cephalothin-resistant gram-negative bacilli. the types of infections treated included those of skin or skin structure, bone, urinary tract, and respiratory tract. in 83% of p. aeruginosa infections, a favorable clinical outcome occurred, compared with 85% for all infections. failure to achieve a cure correlated with the emergence of resistant p. aeruginosa and acinetob ... | 1985 | 2939795 |
| [evolution of acinetobacter calcoaceticus in the hospital milieu, from 1971 to 1984]. | during the last 10 years the authors have evaluated the increasing part played by acinetobacter calcoaceticus in nosocomial infections and the increasing resistance of this species to antibiotics. the study involved 850 clinical strains isolated from 1971 to 1984, and 24 antibiotics were tested. a progressive increase in resistance to beta-lactam antibiotics, aminoglycosides and tetracycline was observed, and to date up to 80-90% of the strains are resistant to all but major drugs such as imipen ... | 1985 | 2935831 |
| in vitro activities of ciprofloxacin, ofloxacin, norfloxacin and rosoxacin compared with cinoxacin and trimethoprim. | the in vitro activities of ciprofloxacin, ofloxacin, norfloxacin, rosoxacin, cinoxacin and trimethoprim have been compared. an agar dilution method has been employed for the measurement of minimal inhibitory concentrations (mics). 426 clinical, bacterial urinary isolates mainly from hospitalised patients were included; all common urinary tract pathogens were represented. the newer quinolones were highly active against enterobacteriaceae species, pseudomonas aeruginosa and acinetobacter calcoacet ... | 1985 | 2934234 |
| epidemic bloodstream infections associated with pressure transducers: a persistent problem. | twenty-four outbreaks of nosocomial bloodstream infection (bsi) were investigated by the centers for disease control from jan 1, 1977 to dec 31, 1987. intravascular pressure monitoring devices (transducers) were the most commonly identified source of bacterial and fungal bsi outbreaks and were implicated as the source of infection in eight (33%) outbreaks. these included outbreaks caused by candida parapsilosis (2), serratia marcescens (2), klebsiella oxytoca (1), pseudomonas cepacia (1), acinet ... | 1989 | 2926106 |
| a study of the value of electrophoretic and other techniques for typing acinetobacter calcoaceticus. | forty-four isolates of acinetobacter calcoaceticus var anitratus collected during hospital outbreaks were studied using polyacrylamide gel electrophoresis (page), plasmid analysis, antibiograms and biochemical tests to determine their degree of similarity. reproducibility tests were also carried out on the page and biochemical techniques to determine their validity when used to compare bacteria of the same type isolated intermittently. page data was analysed densitometrically and isolates compar ... | 1988 | 2907333 |
| endemic occurrence of acinetobacter calcoaceticus biovar anitratus in an intensive care unit. | 1988 | 2905720 | |
| hand disinfection: a comparison of various agents in laboratory and ward studies. | the efficacy of 14 handwashing or disinfectant preparations was compared in laboratory tests on staff volunteers. the test organism, escherichia coli, was applied to the fingertips and log reductions (lr) were measured following treatment with the test agent and control preparations (70% isopropanol and non-medicated bar soap). alcoholic preparations, particularly n-propanol and isopropanol were the most effective showing lrs of 3.1-3.8. chlorhexidine (lr 2.9) and povidone-iodine detergent prepa ... | 1988 | 2899107 |
| [alpha-aminomethylketones as inhibitors of a membrane-bound alanine aminopeptidase]. | methyl ketone derivatives of l-amino acids are substrate analogous inhibitors of membrane bound alanine aminopeptidase (ec 3.4.11.2) from acinetobacter calcoaceticus. the type of inhibition is competitive. compounds with a branched aliphatic side chain are most effective so that valine methyl ketone was found to be the best inhibitor of the enzyme with a k-value of 5.5.10(-7) mol.l-1. | 1987 | 2895641 |
| acinetobacter calcoaceticus foot infection secondary to high-pressure injection injury: a case report. | injection injuries are surgical emergencies occurring most often in the hand and frequently associated with widespread tissue necrosis and infection. this report presents a case of high-pressure injection injury of the foot associated with extensor hallucis longus laceration and infection with acinetobacter calcoaceticus variant anitratus. this injury occurred with a high-pressure "water-blaster" tool used to remove paint from pavement. similar injuries in the foot have not been reported. a. cal ... | 1988 | 2895055 |
| endemic occurrence of acinetobacter calcoaceticus biovar anitratus in an intensive care unit. | one strain of acinetobacter calcoaceticus biovar anitratus caused colonization of 111 patients admitted to an intensive care unit (icu) during a 2-year period. all patients were intubated and had received antibiotic therapy prior to colonization. morbidity due to the organism was about 1%. the colonization rate showed a decreasing trend during the study period, but no seasonal variation. the strain was found in the air in a low concentration and on the hands of 8-13% of the members of the staff. ... | 1987 | 2891755 |
| biotyping of acinetobacter calcoaceticus using the api 2one system. | the api 2one system for the identification of non-fermentative gram-negative bacilli enables the discrimination of a possible 209 different biotypes of acinetobacter spp. and consequently has potential for use as an acinetobacter spp. typing system. a total of 122 separate strains of acinetobacter spp. isolated in nottingham hospitals over a 4 year period from a wide variety of clinical specimens, divided into 31 different biotypes which were stable over a 1 year storage period. two biotypes pre ... | 1987 | 2889767 |
| epidemiology of nosocomial infections due to acinetobacter calcoaceticus. | 1987 | 2889762 | |
| defence reactions of glossina morsitans morsitans against different species of bacteria and trypanosoma brucei brucei. | tsetse flies, glossina morsitans morsitans, fed on rats infected with trypanosoma brucei brucei showed wide fluctuations in total and differential haemocyte counts. similar fluctuations occurred in controls fed on non-infected rats and also between the two groups without showing any difference which could be attributed to the infection. trypanosome infection of the tsetse haemocoel occurred in 16.25% of the flies, starting from the second day after feeding on the infected rats, but salivary glan ... | 1986 | 2872785 |
| localization of hydrolytic enzymes in acinetobacter calcoaceticus. | the localization of hydrolytic enzymes, phosphatase, esterase, lipase and palmitoyl-coa hydrolase was analysed in the cytosol, cytoplasmic membrane, periplasmic fraction, outer membrane and culture supernatant in dependence on the growth rate of the bacteria. the unspecific phosphatase was found to be a cytosolic enzyme. a lipase was the only extracellular enzyme detected. the results pointed to a secretion of the lipase into the culture medium via cytoplasmic and outer membrane. the palmitoyl-c ... | 1986 | 2870172 |
| investigation of an outbreak of infection with acinetobacter calcoaceticus in a special care baby unit. | during a period of 6 months, an 'epidemic strain' of acinetobacter calcoaceticus was isolated from 10 pre-term neonates in a special care baby unit (scbu). of these, nine had pulmonary infections. the tenth was found to have conjunctival colonization only. the 'epidemic strain' was characterized by serotyping, biotyping, bacteriocin typing and antibiograms. an identical strain was isolated from an 'ambu' resuscitation device but not from other environmental samples or from staff on the unit. the ... | 1986 | 2870108 |
| epidemic spread of acinetobacter calcoaceticus in a neurosurgical department analyzed by electronic data processing. | an epidemic spread of acinetobacter calcoaceticus var. anitratus in two neurosurgical wards is described retrospectively and prospectively using electronic data processing. isolation of the species from sputum preceded the isolation from csf by 1/2-1 year. control measures directed against spread by air and indirect contact controlled the epidemic. reexamination of 20 selected strains from the epidemic revealed two distinct resistance patterns. | 1985 | 2862191 |
| a search for intermediates in the bacterial biosynthesis of pqq. | studies on the biosynthesis of pyrroloquinoline quinone (pqq) were performed with acinetobacter calcoaceticus pqq- -mutants belonging to genetically different complementation groups. all mutants were unable to grow on l-arabinose, the conversion of this substrate by the organism only occurring via membrane-bound quinoprotein (pqq-containing) glucose dehydrogenase. in general, the same observation and conclusion applied to shikimate and quinate, requiring active quinoprotein quinate dehydrogenase ... | 1988 | 2855587 |
| inactivation of acinetobacter calcoaceticus acetate kinase by diethylpyrocarbonate. | acetate kinase purified from acinetobacter calcoaceticus was inhibited by diethylpyrocarbonate with a second-order rate constant of 620 m-1.min-1 at ph 7.4 at 30 degrees c and showed a concomitant increase in absorbance at 240 nm due to the formation of n-carbethoxyhistidyl derivative. activity could be restored by hydroxylamine and the ph curve of inactivation indicates the involvement of a residue with a pka of 6.64. complete inactivation of acetate kinase required the modification of seven re ... | 1988 | 2844264 |
| cosmid cloning of five zymomonas trp genes by complementation of escherichia coli and pseudomonas putida trp mutants. | a library of zymomonas mobilis genomic dna was constructed in the broad-host-range cosmid plafr1. the library was mobilized into a variety of escherichia coli and pseudomonas putida trp mutants by using the helper plasmid prk2013. five z. mobilis trp genes were identified by the ability to complement the trp mutants. the trpf, trpb, and trpa genes were on one cosmid, while the trpd and trpc genes were on two separate cosmids. the organization of the z. mobilis trp genes seems to be similar to th ... | 1988 | 2838460 |
| cloning of the gene encoding quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus: evidence for the presence of a second enzyme. | we cloned the gene coding for the quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus. this clone complements gdh mutations in a. calcoaceticus, pseudomonas aeruginosa, and escherichia coli. the gene codes for a protein with an mr of 83,000. evidence is presented for the presence of two different glucose dehydrogenase enzymes in a. calcoaceticus: a protein with an mr of 83,000 and a dimer of two identical subunits with an mr of 50,000. | 1988 | 2834325 |
| virulence genes, borders, and overdrive generate single-stranded t-dna molecules from the a6 ti plasmid of agrobacterium tumefaciens. | agrobacterium tumefaciens transfers the t-dna portion of its ti plasmid to the nuclear genome of plant cells. upon cocultivation of a. tumefaciens a348 with regenerating tobacco leaf protoplasts, six distinct single-stranded t-dna molecules (t strands) were generated in addition to double-stranded t-dna border cleavages which we have previously reported (k. veluthambi, r.k. jayaswal, and s.b. gelvin, proc. natl. acad. sci. usa 84:1881-1885, 1987). the t region of an octopine-type ti plasmid has ... | 1988 | 2832367 |
| cloning and expression in escherichia coli of acinetobacter calcoaceticus genes for benzoate degradation. | the catabolic genes necessary for the conversion of benzoate to catechol have been cloned from acinetobacter calcoaceticus into escherichia coli. the cloned genes, benabcd, encoded both a benzoate 1,2-dioxygenase system, composed of nadh-cytochrome c reductase and terminal oxygenase components, and a cis-diol dehydrogenase. the dioxygenase system appears to be encoded by three genes, benabc, whose products, 53-, 19-, and 38-kilodalton proteins, correspond in size to those of components in other ... | 1987 | 2824437 |
| involvement of a plasmid in growth on and dispersion of crude oil by acinetobacter calcoaceticus ra57. | a crude-oil-degrading acinetobacter species, acinetobacter calcoaceticus ra57, was isolated by standard enrichment culture techniques on the basis of its ability to utilize the oily sludge found in the vicinity of a local gas station. strain ra57 was found to contain four plasmids: psr1 (5.1 kilobases [kb]), psr2 (5.4 kb), psr3 (10.5 kb), and psr4 (20 kb). both supercoiled and open circular forms of the first three plasmids were identified by two-dimensional gel electrophoresis. restriction endo ... | 1987 | 2821903 |
| a novel method for the identification and distinction of the beta-lactamases of the genus acinetobacter. | the characterization of the chromosomal beta-lactamases of acinetobacter has proved difficult because of the poor focusing of these enzymes in conventional isoelectric focusing on polyacrylamide gels. we describe a novel isoelectric focusing method, which employs an agarose gel incorporating a detergent with sorbitol and urea, to examine the beta-lactamases produced by eight clinical strains of acinetobacter calcoaceticus; we have identified four different beta-lactamases. the molecular masses o ... | 1989 | 2808182 |
| the inanimate environment of an intensive care unit as a potential source of nosocomial bacteria: evidence for long survival of acinetobacter calcoaceticus. | environmental surface and personnel hand impression cultures were obtained during 13 sampling periods in the university of virginia pediatric intensive care unit to document potential reservoirs of nosocomial pathogens. in 78 environmental cultures staphylococcus aureus was found eight times and gram-negative bacilli ten times. the patient chart cover was the most commonly contaminated surface. acinetobacter calcoaceticus was found in five of ten cultures positive for gram-negative bacilli. thir ... | 1989 | 2794465 |
| characterization of acinetobacter calcoaceticus catm, a repressor gene homologous in sequence to transcriptional activator genes. | two structural genes needed for catechol degradation, cata and catb, encode the respective enzymes catechol 1,2-dioxygenase (ec 1.13.11.1) and muconate cycloisomerase (ec 5.5.1.1). catechol is an intermediate in benzoate degradation, and the cata and catb genes are clustered within a 17-kilobase-pair (kbp) region of acinetobacter calcoaceticus chromosomal dna containing all of the structural genes required for the conversion of benzoate to tricarboxylic acid cycle intermediates. cata and catb we ... | 1989 | 2793826 |
| novel carbenicillin-hydrolyzing beta-lactamase (carb-5) from acinetobacter calcoaceticus var. anitratus. | a strain of acinetobacter calcoaceticus var. anitratus highly resistant to ticarcillin but susceptible to ticarcillin in combination with clavulanic acid (2 mg/l) was found to produce a constitutive beta-lactamase. this enzyme was periplasmic with a characteristic substrate profile of a carbenicillin-hydrolyzing enzyme. enzyme inhibition was detected with antiserum (anti-carb-3), pcmb, cloxacillin, clavulanic acid and sulbactam. this novel enzyme with a molecular mass of 28,000 resembles other p ... | 1989 | 2786828 |
| susceptibility to antibiotics in acinetobacter calcoaceticus. | 1989 | 2784129 | |
| purification and properties of a cephalosporinase from acinetobacter calcoaceticus. | 1989 | 2784128 | |
| haem-containing protein complexes of acinetobacter calcoaceticus as secondary electron acceptors for quinoprotein glucose dehydrogenase. | 1989 | 2774522 | |
| use of protein profiles to identify acinetobacter calcoaceticus in a respiratory care unit. | the presence of acinetobacters in a respiratory care unit was prospectively studied because of an increase in the number of isolations of acinetobacter calcoaceticus. cell envelope protein electrophoresis was used to distinguish strains. eleven protein patterns were observed in isolates from patients and their environment. one pattern (pattern 1) was seen in several patients and environmental samples. another pattern (pattern 2) was identified repeatedly in samples from skin and mucous membranes ... | 1989 | 2768524 |
| susceptibility of acinetobacter calcoaceticus to antimicrobial drugs, alone and combined, with and without defibrinated human blood. | twenty-five clinical isolates of acinetobacter calcoaceticus were examined for susceptibility to 14 antimicrobial drugs. in terms of inhibitory and bactericidal activities, imipenem and polymyxin b were most active, followed by amikacin and ceftazidime. four isolates were resistant against fluoroquinolones (ciprofloxacin, norfloxacin, ofloxacin). the isolates varied in susceptibility to aztreonam, cefotaxime, cotrimoxazole, gentamicin, mezlocillin, netilmicin and piperacillin. fresh defibrinated ... | 1989 | 2758872 |
| [nosocomial infections at the university hospital of caracas]. | over a period of one year at the university hospital of caracas, a program of epidemiological surveillance was carried out on nosocomial infections. a total of 1556 cases were identified in 913 hospitalized patients. the incidence of nosocomial infections was 74 cases per 1000 hospital discharges. cutaneous, surgical wound, lower respiratory and urinary tract infections were the most frequently identified. the intensive care unit, the neonatal pediatric service and the three medicine services re ... | 1989 | 2748851 |
| laboratory investigation of hospital outbreak caused by two different multiresistant acinetobacter calcoaceticus subsp. anitratus strains. | during a 7-month period, from december 1986 to june 1987, multiresistant strains of acinetobacter calcoaceticus subsp. anitratus were isolated from 25 patients in a respiratory intensive care unit. the biochemical characteristics defined two groups of strains, group 1 (14 strains) and group 2 (11 strains). both groups had the same biochemical characteristics, but group 2 strains could assimilate adipate and phenyl acetate. moreover, of 16 antibiotics tested only netilmicin and imipenem had some ... | 1989 | 2745682 |
| interactions between lipopolysaccharide and outer membrane proteins of acinetobacter calcoaceticus studied by an affinity electrophoresis system. | r-form lipopolysaccharides of acinetobacter calcoaceticus could be incorporated into polyacrylamide gels in an immobile form by adding it directly to the acrylamide-n,n'-methylenebisacrylamide polymerization mixture. the separation of a. calcoaceticus 69 v outer membrane proteins in these affinity gels demonstrated a specific interaction with the lipopolysaccharide ligand for one of the proteins. this protein is heat-modifiable and has an mr of about 18,000. by incorporation of varying concentra ... | 1989 | 2743966 |
| effects of growth rate and oxygen tension on glucose dehydrogenase activity in acinetobacter calcoaceticus lmd 79.41. | the regulation of the synthesis of the quinoprotein glucose dehydrogenase (ec 1.1.99.17) has been studied in acinetobacter calcoaceticus lmd 79.41, an organism able to oxidize glucose to gluconic acid, but unable to grow on both compounds. glucose dehydrogenase was synthesized constitutively in both batch and carbon-limited chemostat cultures on a variety of substrates. in acetate-limited chemostat cultures glucose dehydrogenase levels and the glucose-oxidizing capacity of whole cells were depen ... | 1989 | 2742367 |
| control of an epidemic spread of a multi-resistant strain of acinetobacter calcoaceticus in a hospital. | the spread of a multi-resistant glucose-acidifying acinetobacter calcoaceticus strain in a community hospital was studied. after admission of a colonized patient to the hospital the strain was found in clinical specimens from icu patients and subsequently from several of these patients after transfer to medical wards. environmental specimens from the icu and medical wards were analysed in order to investigate the mode of spread of the strain. isolates of a. calcoaceticus were screened by their a ... | 1989 | 2738220 |
| a seven year survey of antibiotic susceptibility and its relationship with usage. | bacterial susceptibilities to 14 antibiotics of 7385 clinical isolates, belonging to six species of gram-negative bacilli, were analysed during seven years (1980-86). the recovery of serratia marcescens, acinetobacter calcoaceticus and pseudomonas aeruginosa increased while their sensitivity to antibiotics decreased significantly, especially to aminoglycosides and tetracycline. there were significant correlations between increase in antibiotic use and decrease in susceptibility. there was a stri ... | 1989 | 2732126 |
| multiple forms of carboxylesterase activity in acinetobacter calcoaceticus. | a carboxylesterase activity (e.c.3.1.1) was found in all four strains of acinetobacter calcoaceticus tested. the activity was present in both 2 x 10(4) gav h-1 supernatant and bacterial wall-membrane fractions. the activity in the supernatant was in two molecular weight forms, the predominant form with a mr of about 10(3) k and a minor form mr approximately 600 k. the activity was inhibited by phenylmethylsulfonyl fluoride. sds-page showed that in a. calcoaceticus ncib 8250 the activity was comp ... | 1989 | 2721925 |
| characterization of a periplasmic insulin-cleaving metalloproteinase from acinetobacter calcoaceticus. | in acinetobacter calcoaceticus, a gram-negative bacterial species, a soluble insulin-degrading proteinase, located in the periplasm as well as in the cytosol, could be established. the periplasmic and cytosolic enzymes agree in their inhibition pattern, ph-optimum and molecular weight. the insulin-degrading enzyme of acinetobacter calcoaceticus resembles the corresponding proteinases of escherichia coli. it is a metalloproteinase with a ph-optimum in the neutral range and can be reactivated by d ... | 1989 | 2695074 |
| [identification and localization of exopeptidase activities bound to membranes of acinetobacter calcoaceticus]. | after gel filtration the supernatant of triton x-100 treated membrane sediments of acinetobacter calcoaceticus shows activities for alanyl aminopeptidase, leucyl aminopeptidase, glutamyl aminopeptidase, prolyl aminopeptidase, aminopeptidase my, gamma-glutamyl arylamidase, dipeptidylpeptidase iv and prolyl carboxypeptidase. the intracellular localization of the enzymes was analyzed by sucrose density gradient centrifugation of the dnase/rnase pretreated membrane sediments. most of the exopeptidas ... | 1989 | 2695071 |
| a comparative evaluation of oral ofloxacin versus intravenous cefotaxime therapy for serious skin and skin structure infections. | in a single-blind, placebo-controlled randomized trial, 100 successive patients were enrolled with serious skin and soft-tissue infections, whose illnesses had precipitated an initial hospital admission or an extension of inpatient care. there were 93 evaluable patients who received either ofloxacin, 400 mg orally every 12 hours plus an intravenously administered placebo every eight hours, or cefotaxime, 2.0 g intravenously every eight hours plus an orally administered placebo every 12 hours. th ... | 1989 | 2690621 |
| intravenous sulbactam/ampicillin in the treatment of pediatric infections. | a total of 82 patients involving 83 episodes of proven or presumed bacterial infection were treated with sulbactam/ampicillin. these included 36 cases of soft tissue infection or abscess, four cases of joint or bone infection, 20 cases of respiratory tract infection (17 cases of pneumonia, two of otitis media, and one of tonsillitis), 15 urinary tract infections, three cases of enterocolitis, one case of infective endocarditis, two cases of septicemia, and two of peritonitis. the causative patho ... | 1989 | 2686918 |
| firefly luciferase as a reporter enzyme for measuring gene expression in vegetative and symbiotic rhizobium meliloti and other gram-negative bacteria. | a dna segment carrying a cdna copy of the luciferase gene (luc) of the north american firefly photinus pyralis, fused to the lambda pr promoter and expressed in escherichia coli [de wet et al., proc. natl. acad. sci. usa 82 (1985) 7870-7873], was inserted into a broad-host-range plasmid vector and established in a variety of gram-negative bacteria. luciferase activity, expressed from the lambda pr promoter, was detected in both intact cells and extracts prepared from cells of strains of rhizobiu ... | 1989 | 2680767 |
| cloning of the genes encoding the two different glucose dehydrogenases from acinetobacter calcoaceticus. | glucose dehydrogenase (gdh) is a pqq dependent bacterial enzyme which converts aldoses to their corresponding acids. a. calcoaceticus contains two different pqq dependent glucose dehydrogenases designated gdh-a which is active in vivo and gdh-b of which only in vitro activity can be shown. we cloned the genes coding for the two gdh enzymes. the dna sequences of both gdh genes were determined. there is no obvious homology between gdha and gdhb. both gdh enzymes oxidize d-glucose in vitro but disa ... | 1989 | 2673030 |
| cloning, characterization and dna sequencing of the gene encoding the mr 50,000 quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus. | recently we described the cloning of the gene coding for a mr 87,000 glucose dehydrogenase (gdh-a) from acinetobacter calcoaceticus. in this report we describe the cloning of a gene coding for a second gdh (gdh-b) with a mr of 50,000 from the same organism. this gene was isolated using a 20-mer synthetic oligonucleotide, derived from the n-terminal amino acid sequence of purified gdh-b as a probe to screen a genomic bank. from the dna sequence of the gdhb gene, a protein can be derived of mr 52, ... | 1989 | 2671663 |
| [the effect of thiocyanate on bacterial plasmids using the plasmid screening test]. | a plasmid screening test (alkaline lysis and agarose gel electrophoresis) was used to assess the effect of thiocyanate on the plasmid replication on two bacterial strains with plasmids of different size, klebsiella pneumoniae and acinetobacter calcoaceticus. at concentrations physiologic for mammals (5-50 mg scn-/l) no effect on the replication of extrachromosomal dna was noted. this reaffirms the concept that a physiologic anion may be responsible for biological regulatory processes but will no ... | 1989 | 2665338 |
| [susceptibilities of clinical isolates to antibacterial agents. a study mainly focused on ofloxacin (the second report). reported by the research group for testing ofloxacin susceptibility on clinical isolates]. | susceptibilities of various clinical isolates to ofloxacin (oflx) and other antibacterial drugs were examined at 128 hospital laboratories in 36 prefectures throughout japan between april, 1986 and march, 1987. the results were totalized with an emphasis mainly on oflx and were compared with data obtained in the previous year. in this study, identification and susceptibility tests of the isolates were carried out at each hospital laboratory and the tests were performed according to the 1-dilutio ... | 1989 | 2664255 |
| cloning of a carbofuran hydrolase gene from achromobacter sp. strain wm111 and its expression in gram-negative bacteria. | a 14-kilobase-pair (kbp) ecori dna fragment that encodes an enzyme capable of rapid hydrolysis of n-methylcarbamate insecticides (carbofuran hydrolase) was cloned from carbofuran-degrading achromobacter sp. strain wm111. when used to probe southern blots containing plasmid and total dnas from wm111, this 14-kbp fragment hybridized strongly to a 14-kbp ecori fragment from the greater than 100-kbp plasmid harbored by this strain but weakly to ecori-digested total dna from achromobacter sp. strain ... | 1989 | 2661544 |
| therapeutic efficacy of the combination of aztreonam with cefotaxime in the treatment of severe nosocomial pneumonia. comparative study against amikacin combined with cefotaxime. | the combination aztreonam + cefotaxime (az + ce) was compared to amikacin + cefotaxime (am + ce) in the treatment of nosocomial pneumonia acquired at the intensive-care unit. this study included a total of 33 patients fulfilling criteria for nosocomial pneumonia. 16 of them were randomly allocated to the az + ce group and 17 to the am + ce group. the empirical treatment was effective for 78% of az + ce cases and 92% of am + ce cases (p = ns). clinical care was observed in 77% of cases (10 out of ... | 1989 | 2659289 |
| extracellular product of nocardia amarae induces bacterial cell flocculation. | the fact that nocardia amarae yk1 produced a bacterial flocculation-inducing substance (designated as fix) was discovered. fix had a function of flocculating proliferous cells. fix-induced flocculation was inhibited by making cells resting, but not completely by adding chloramphenicol. fix worked widely on gram-positive to -negative bacteria. in the presence of fix, achromobacter cycloclastus iam1013, acinetobacter calcoaceticus iam1517, bacillus subtilis iam1069, escherichia coli c600-1, e. col ... | 1989 | 2653957 |
| a clinical evaluation of sulbactam/ampicillin in the treatment of pediatric infections. | we have treated 42 episodes of pediatric infections with sulbactam/ampicillin since 1987. included were 9 cellulitis, 9 urinary tract infections, 5 cervical lymphadenitis, 4 meningitis, 2 thoracic empyema, 2 osteomyelitis, 2 sepsis, 1 furuncle, 1 perianal abscess, 1 dental abscess, 1 peritonsillitis, 1 salmonellosis, 1 shigellosis, 1 peritonitis, 1 suppurative thyroiditis, 1 infective endocarditis. responsible pathogens were escherichia coli in 8, staphylococcus aureus in 6, hemophilus influenza ... | 1989 | 2637593 |
| antimicrobial activity of dactimicin in vitro compared with that of dibekacin, netilmicin, sisomicin and micronomicin. | antimicrobial activity of dactimicin, a pseudo-disaccharide aminoglycoside antibiotic, was compared with those of dibekacin, netilmicin, sisomicin and micronomicin using clinical isolates of four gram-positive and sixteen gram-negative bacteria. dactimicin was more active than the reference amino-glycosides against serratia marcescens, especially gentamicin-resistant serratia sp., proteus vulgaris, p. rettgeri and klebsiella oxytoca, but less active against pseudomonas aeruginosa and p. mirabili ... | 1989 | 2632214 |
| lipopolysaccharide-protein interactions: determination of dissociation constants by affinity electrophoresis. | an affinity electrophoresis system is described to allow determination of dissociation constants of lipopolysaccharide (lps)-protein complexes. the lps ligand is incorporated into polyacrylamide gels by addition to the polyacrylamide-n,n'-methylenebisacrylamide polymerization mixture. quantitative evaluation revealed formation of immobile protein-ligand complexes. the method was applied both to r- and s-form lps from acinetobacter calcoaceticus. for a heat-modifiable outer membrane protein with ... | 1989 | 2612487 |
| purification and characterization of benzaldehyde dehydrogenase i from acinetobacter calcoaceticus. | benzaldehyde dehydrogenase i was purified from acinetobacter calcoaceticus by deae-sephacel, phenyl-sepharose and f.p.l.c. gel-filtration chromatography. the enzyme was homogeneous and completely free from the isofunctional enzyme benzaldehyde dehydrogenase ii, as judged by denaturing and non-denaturing polyacrylamide-gel electrophoresis. the subunit mr value was 56,000 (determined by sds/polyacrylamide-gel electrophoresis). estimations of the native mr value by gel-filtration chromatography gav ... | 1989 | 2597133 |
| [acinetobacter calcoaceticus as a cause of infection in wounds and burns]. | over the period from january 1985 to july 1986 2038 samples of the patients with open wounds and burns, treated in the military medical academy in belgrade, were tested. acinetobacter calcoaceticus was found in 152 samples (7.45%). the sensitivity to standard hemotherapeutics for gram-negative bacteria was assessed. results indicated that acinetobacter calcoaceticus showed a high resistance to all hemotherapeutics used in this investigation. even if it was a opportunistic pathogen bacterium its ... | 1989 | 2595461 |
| nosocomial infections due to acinetobacter calcoaceticus. | fifty four isolates of acinetobacter calcoaceticus were studied in a period of 6 months. maximum isolates were from burns cases and environmental sampling from burns ward also grew the same organism, indicating their role as nosocomial pathogen. acinetobacter may initially be mistaken for neisseria species. as the organisms show multidrug resistance to commonly used antibiotics their correct identification is important. | 1989 | 2585331 |
| [a membrane-bound alanine aminopeptidase from acinetobacter calcoaceticus. 2. substrate specificity of the enzyme]. | the substrate specificity of the membrane-bound alanine aminopeptidase from acinetobacter calcoaceticus was investigated with a series of substituted amides of alpha-amino acids. in contrast to mammalian aap forms, the rate of hydrolysis of the aap from acinetobacter calcoaceticus is higher using 4-nitranilide than 2-naphthylamide substrates. the enzyme affinity is very high for alanine substrates and decreases in the series of the amide substituents, from methyl coumarylamides, 4-nitranilides, ... | 1989 | 2575905 |
| [a membrane-bound alanine aminopeptidase from acinetobacter calcoaceticus. 1. isolation and purification of the enzyme]. | the alanine aminopeptidase of acinetobacter calcoaceticus was found to be bound to the inner membranes only. the enzyme was solubilized by triton x-100 and purified approximately 480-fold by gel filtration and affinity chromatography on alanine methyl ketone-ah-sepharose 4b. the purified alanine aminopeptidase has a molecular mass of 212 kda, estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the enzyme selectively catalyses the hydrolysis of n-terminal alanine residues of p ... | 1989 | 2575904 |
| an outbreak of acinetobacter septicaemia in a neonatal intensive care unit. | we describe an outbreak of gram-negative septicaemia due to a rare, non-fermenting, aerobic organism, acinetobacter calcoaceticus var. lwoffi. the outbreak occurred on a neonatal unit and was confined to babies who were receiving parenteral nutrition. seven babies developed septicaemia within 24 hours. the source of the outbreak was never firmly established, but contamination of the parenteral nutrition fluid was considered most likely. all 7 babies recovered uneventfully after a week's course o ... | 1989 | 2575636 |
| epidemic iatrogenic acinetobacter spp. meningitis following administration of intrathecal methotrexate. | we report the first outbreak of acinetobacter species meningitis in a group of children with acute leukaemia following the administration of intrathecal chemotherapy. eight of twenty patients receiving methotrexate injections on a single day developed signs and symptoms of meningitis within 18 h of treatment, and cases were clustered by time of administration. a cohort study comparing case and non-case patients did not identify any specific host factor associated with meningitis. acinetobacter c ... | 1989 | 2575105 |
| frequency of plasmids in strains of acinetobacter calcoaceticus. | plasmids were found in 75 out of 93 clinical isolates of acinetobacter calcoaceticus. the strains harboured up to 20 plasmids each, with two-thirds containing two or more. most plasmids were smaller than 15 md. strains of a. iwoffi contained more plasmids than a. anitratus. plasmid analysis may be an easy and valuable tool in the epidemiology of a. calcoaceticus, especially in conjunction with other typing methods. | 1989 | 2570099 |
| acinetobacter calcoaceticus biovar anitratus septicaemia in a neonatal intensive care unit: epidemiology and control. | nineteen neonates with septicaemia caused by acinetobacter calcoaceticus biovar anitratus were treated in a neonatal intensive care unit between october, 1983 and march, 1986. the ages of the patients at the onset of septicaemia ranged from 4 to 22 days (mean 9.7 days). their birth weights ranged from 1000 g to 3350 g (mean 1790 g) and were less than 2000 g in 14 patients. antibiotics had been administered to 17 of the 19 neonates before the onset of septicaemia, and all mature infants had recei ... | 1989 | 2570098 |
| in vitro activity of at-4140 against clinical bacterial isolates. | the activity of at-4140, a new fluoroquinolone, was evaluated against a wide range of clinical bacterial isolates and compared with those of existing analogs. at-4140 had a broad spectrum and a potent activity against gram-positive and -negative bacteria, including legionella spp. and bacteroides fragilis. the activity of at-4140 against gram-positive and -negative cocci, including acinetobacter calcoaceticus, was higher than those of ciprofloxacin, ofloxacin, and norfloxacin. its activity again ... | 1989 | 2558617 |
| quinoprotein d-glucose dehydrogenase of the acinetobacter calcoaceticus respiratory chain: membrane-bound and soluble forms are different molecular species. | acinetobacter calcoaceticus is known to contain soluble and membrane-bound quinoprotein d-glucose dehydrogenases, while other oxidative bacteria contain the membrane-bound enzyme exclusively. the two forms of glucose dehydrogenase were believed to be the same enzyme or interconvertible forms. previously, matsushita et al. [(1988) fems microbiol. lett 55, 53-58] showed that the two enzymes are different with respect to enzymatic and immunological properties, as well as molecular weight. in the pr ... | 1989 | 2551369 |
| use of low-frequency-cleavage restriction endonucleases for dna analysis in epidemiological investigations of nosocomial bacterial infections. | epidemiological investigations of bacterial infections are generally based on multiple phenotypic markers that are often difficult to verify. a more general and reliable method is genomic dna analysis by restriction endonucleases. however, the commonly used endonucleases produce too many fragments for correct separation by agarose electrophoresis. in contrast, simple electrophoretic patterns are obtained after genomic dna digestion by low-frequency-cleavage restriction endonucleases and pulsed-f ... | 1989 | 2550517 |
| reversible thermal inactivation of the quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus. ca2+ ions are necessary for re-activation. | the soluble form of the homogeneous quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus is reversibly inactivated at temperatures above 35 degrees c. an equilibrium is established between active and denatured enzyme, this depending on the protein concentration and the inactivation temperature used. upon thermal inactivation the enzyme dissociates into the prosthetic group pyrroloquinoline quinone and the apo form of glucose dehydrogenase. after inactivation at 50 degrees c active ... | 1989 | 2549970 |
| genes involved in the biosynthesis of pqq from acinetobacter calcoaceticus. | from a gene bank of the acinetobacter calcoaceticus genome a plasmid was isolated that complements four different classes of pqq- mutants. subclones of this plasmid revealed that the four corresponding pqq genes are located on a fragment of 5 kilobases. the nucleotide sequence of this 5 kb fragment was determined and by means of tn5 insertion mutants the reading frames of the pqq genes could be identified. three of the pqq genes code for proteins of mr 29700 (gene i), mr 10800 (gene ii) and mr 4 ... | 1989 | 2549866 |
| quinoprotein d-glucose dehydrogenases in acinetobacter calcoaceticus lmd 79.41: purification and characterization of the membrane-bound enzyme distinct from the soluble enzyme. | acinetobacter calcoaceticus is known to contain soluble and membrane-bound quinoprotein d-glucose dehydrogenases while other oxidative bacteria such as pseudomonas or gluconobacter contain only membrane-bound enzyme. the two different forms were believed to be the same enzyme or interconvertible. present results show that the two different forms of glucose dehydrogenase are distinct from each other in their enzymatic and immunological properties as well as in their molecular size. the soluble an ... | 1989 | 2549865 |
| physiological significance and bioenergetic aspects of glucose dehydrogenase. | the regulation of the pqq-linked glucose dehydrogenase in different organisms is reviewed. it is concluded that this enzyme functions as an auxiliary energy-generating mechanism, because it is maximally synthesized under conditions of energy stress. it is now definitively established that the oxidation of glucose to gluconate generates metabolically useful energy. the magnitude of the contribution of the oxidation of glucose to gluconate via this enzyme to the growth yield of organisms such as a ... | 1989 | 2549864 |