Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| detection of foodborne bacterial pathogens from individual filth flies. | there is unanimous consensus that insects are important vectors of foodborne pathogens. however, linking insects as vectors of the pathogen causing a particular foodborne illness outbreak has been challenging. this is because insects are not being aseptically collected as part of an environmental sampling program during foodborne outbreak investigations and because there is not a standardized method to detect foodborne bacteria from individual insects. to take a step towards solving this problem ... | 2015 | 25742168 |
| non-selective regulation of peroxide and superoxide resistance genes by perr in campylobacter jejuni. | campylobacter jejuni is an important foodborne pathogen. the molecular mechanisms for the regulation of oxidative stress resistance have not yet been understood fully in this bacterium. in this study, we investigated how perr (peroxide stress regulator) modulates the transcriptional regulation of both peroxide and superoxide resistance genes in c. jejuni, particularly under oxidative stress conditions. the transcriptional levels of ahpc, kata, and sodb were substantially increased by aeration an ... | 2015 | 25741333 |
| multicenter evaluation of the bd max enteric bacterial panel pcr assay for rapid detection of salmonella spp., shigella spp., campylobacter spp. (c. jejuni and c. coli), and shiga toxin 1 and 2 genes. | diarrhea due to enteric bacterial pathogens causes significant morbidity and mortality in the united states and worldwide. however, bacterial pathogens may be infrequently identified. currently, culture and enzyme immunoassays (eias) are the primary methods used by clinical laboratories to detect enteric bacterial pathogens. we conducted a multicenter evaluation of the bd max enteric bacterial panel (ebp) pcr assay in comparison to culture for the detection of salmonella spp., shigella spp., cam ... | 2015 | 25740779 |
| assessing the public health risk of shiga toxin-producing escherichia coli by use of a rapid diagnostic screening algorithm. | shiga toxin-producing escherichia coli (stec) is an enteropathogen of public health concern because of its ability to cause serious illness and outbreaks. in this prospective study, a diagnostic screening algorithm to categorize stec infections into risk groups was evaluated. the algorithm consists of prescreening stool specimens with real-time pcr (qpcr) for the presence of stx genes. the qpcr-positive stool samples were cultured in enrichment broth and again screened for stx genes and addition ... | 2015 | 25740764 |
| gastrointestinal infections and diarrheal disease in ghanaian infants and children: an outpatient case-control study. | diarrheal diseases are among the most frequent causes of morbidity and mortality in children worldwide, especially in resource-poor areas. this case-control study assessed the associations between gastrointestinal infections and diarrhea in children from rural ghana. | 2015 | 25738935 |
| antiretroviral therapy (art) use, human immunodeficiency virus (hiv)-1 rna suppression, and medical causes of hospitalization among hiv-infected intravenous drug users in the late art era. | antiretroviral therapy (art) has reduced the rates and changed the causes of hospital admission. however, human immunodeficiency virus-positive intravenous drug users (hiv-idu) continue to have increased hospitalizations and discharge diagnosis are less defined in the late art era. our aim was to examine art use, hiv-1 rna suppression, and hospital discharge diagnoses among hiv-idu admitted to an urban hospital. | 2014 | 25734084 |
| mind-like atpase flhg effects location and number of bacterial flagella during c-ring assembly. | the number and location of flagella, bacterial organelles of locomotion, are species specific and appear in regular patterns that represent one of the earliest taxonomic criteria in microbiology. however, the mechanisms that reproducibly establish these patterns during each round of cell division are poorly understood. flhg (previously ylxh) is a major determinant for a variety of flagellation patterns. here, we show that flhg is a structural homolog of the atpase mind, which serves in cell-divi ... | 2015 | 25733861 |
| identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction. | campylobacter jejuni (c. jejuni) and campylobacter coli (c. coli) are the most common bacterial causes of enterocolitis in humans. however, identification of the species level is not always possible using standard biochemical tests. | 2015 | 25731001 |
| infestation of research zebra finch colony with 2 novel mite species. | a zebra finch (taeniopygia guttata) housed in a neuroscience laboratory was observed to have numerous feather mites. subsequently, similar mites were found on other birds in the animal facility and research space. the most abundant mite was a novel, undescribed species in the genus neocheyletiella. whereas known neocheyletiella mites have previously been characterized as skin parasites of various birds worldwide, the species on the zebra finches is unique because it lives and builds nests in the ... | 2015 | 25730757 |
| helicobacter pylori: genomic insight into the host-pathogen interaction. | the advent of genomic analyses has revolutionized the study of human health. infectious disease research in particular has experienced an explosion of bacterial genomic, transcriptomic, and proteomic data complementing the phenotypic methods employed in traditional bacteriology. together, these techniques have revealed novel virulence determinants in numerous pathogens and have provided information for potential chemotherapeutics. the bacterial pathogen, helicobacter pylori, has been recognized ... | 2015 | 25722969 |
| guillain - barre syndrome in a patient with acute myocardial infarction with ventricular septal defect repair treated with plasma exchange. | guillain - barre syndrome (gbs) is an acute, frequently severe progressive illness of peripheral nervous system that is autoimmune in nature. gbs after myocardial infarction (mi) with ventricular septal defect (vsd) is uncommon with high mortality rate if not treated promptly.[1] we report a successful outcome of gbs post mi with vsd in a 60-year-old male patient who was on a ventilator treated successfully with therapeutic plasma exchange. | 2015 | 25722582 |
| mass spectrometry-based proteomic approaches to study pathogenic bacteria-host interactions. | elucidation of molecular mechanisms underlying host-pathogen interactions is important for control and treatment of infectious diseases worldwide. within the last decade, mass spectrometry (ms)-based proteomics has become a powerful and effective approach to better understand complex and dynamic host-pathogen interactions at the protein level. herein we will review the recent progress in proteomic analyses towards bacterial infection of their mammalian host with a particular focus on enteric pat ... | 2015 | 25722051 |
| fecal shedding of thermophilic campylobacter in a dairy herd producing raw milk for direct human consumption. | factors affecting the fecal shedding of thermophilic campylobacter in italian dairy farms were investigated in a 12-month longitudinal study performed on a dairy farm authorized to sell raw milk in italy. fifty animals were randomly selected from 140 adult and young animals, and fecal samples were collected six times at 2-month intervals. at each sampling time, three trough water samples and two trough feed samples also were collected for both adult and young animals. samples were analyzed with ... | 2015 | 25719884 |
| synergistic antimicrobial activity of camellia sinensis and juglans regia against multidrug-resistant bacteria. | synergistic combinations of antimicrobial agents with different mechanisms of action have been introduced as more successful strategies to combat infections involving multidrug resistant (mdr) bacteria. in this study, we investigated synergistic antimicrobial activity of camellia sinensis and juglans regia which are commonly used plants with different antimicrobial agents. antimicrobial susceptibility of 350 gram-positive and gram-negative strains belonging to 10 different bacterial species, was ... | 2015 | 25719410 |
| a surprising range of modified-methionyl s-adenosylmethionine analogues support bacterial growth. | s-adenosyl-l-methionine (adomet) is an essential metabolite, serving in a very wide variety of metabolic reactions. the enzyme that produces adomet from l-methionine and atp (methionine adenosyltransferase, mat) is thus an attractive target for antimicrobial agents. we previously showed that a variety of methionine analogues are mat substrates, yielding adomet analogues that function in specific methyltransfer reactions. however, this left open the question of whether the modified adomet molecul ... | 2015 | 25717169 |
| vaccines for viral and bacterial pathogens causing acute gastroenteritis: part ii: vaccines for shigella, salmonella, enterotoxigenic e. coli (etec) enterohemorragic e. coli (ehec) and campylobacter jejuni. | in part ii we discuss the following bacterial pathogens: shigella, salmonella (non-typhoidal), diarrheogenic e. coli (enterotoxigenic and enterohemorragic) and campylobacter jejuni. in contrast to the enteric viruses and vibrio cholerae discussed in part i of this series, for the bacterial pathogens described here there is only one licensed vaccine, developed primarily for vibrio cholerae and which provides moderate protection against enterotoxigenic e. coli (etec) (dukoral(®)), as well as a few ... | 2015 | 25715096 |
| vaccines for viral and bacterial pathogens causing acute gastroenteritis: part i: overview, vaccines for enteric viruses and vibrio cholerae. | efforts to develop vaccines for prevention of acute diarrhea have been going on for more than 40 y with partial success. the myriad of pathogens, more than 20, that have been identified as a cause of acute diarrhea throughout the years pose a significant challenge for selecting and further developing the most relevant vaccine candidates. based on pathogen distribution as identified in epidemiological studies performed mostly in low-resource countries, rotavirus, cryptosporidium, shigella, diarrh ... | 2015 | 25715048 |
| a new player at the flagellar motor: flil controls both motor output and bias. | the bacterial flagellum is driven by a bidirectional rotary motor, which propels bacteria to swim through liquids or swarm over surfaces. while the functions of the major structural and regulatory components of the flagellum are known, the function of the well-conserved flil protein is not. in salmonella and escherichia coli, the absence of flil leads to a small defect in swimming but complete elimination of swarming. here, we tracked single motors of these bacteria and found that absence of fli ... | 2015 | 25714720 |
| lytm proteins play a crucial role in cell separation, outer membrane composition, and pathogenesis in nontypeable haemophilus influenzae. | lytm proteins belong to a family of bacterial metalloproteases. in gram-negative bacteria, lytm factors are mainly reported to have a direct effect on cell division by influencing cleavage and remodeling of peptidoglycan. in this study, mining nontypeable haemophilus influenzae (nthi) genomes, three highly conserved open reading frames (orfs) containing a lytm domain were identified, and the proteins encoded by the orfs were named yeba, envc, and nlpd on the basis of their homology with the esch ... | 2015 | 25714719 |
| microbial aetiology of acute diarrhoea in children under five years of age in khartoum, sudan. | diarrhoea is one of leading causes of morbidity and mortality worldwide. recent estimations suggested the number of deaths is close to 2.5 million. this study examined the causative agents of diarrhoea in children under 5 years of age in suburban areas of khartoum, sudan. a total of 437 stool samples obtained from children with diarrhoea were examined by culture and pcr for bacteria, by microscopy and pcr for parasites and by immunoassay for detection of rotavirus a. of the 437 samples analysed, ... | 2015 | 25713206 |
| golgi anti-apoptotic proteins are highly conserved ion channels that affect apoptosis and cell migration. | golgi anti-apoptotic proteins (gaaps) are multitransmembrane proteins that are expressed in the golgi apparatus and are able to homo-oligomerize. they are highly conserved throughout eukaryotes and are present in some prokaryotes and orthopoxviruses. within eukaryotes, gaaps regulate the ca(2+) content of intracellular stores, inhibit apoptosis, and promote cell adhesion and migration. data presented here demonstrate that purified viral gaaps (vgaaps) and human bax inhibitor 1 form ion channels ... | 2015 | 25713081 |
| analysis of the link between the redox state and enzymatic activity of the htra (degp) protein from escherichia coli. | bacterial htras are proteases engaged in extracytoplasmic activities during stressful conditions and pathogenesis. a model prokaryotic htra (htra/degp from escherichia coli) requires activation to cleave its substrates efficiently. in the inactive state of the enzyme, one of the regulatory loops, termed la, forms inhibitory contacts in the area of the active center. reduction of the disulfide bond located in the middle of la stimulates htra activity in vivo suggesting that this s-s bond may play ... | 2015 | 25710793 |
| proteomic profiling of the outer membrane fraction of the obligate intracellular bacterial pathogen ehrlichia ruminantium. | the outer membrane proteins (omps) of gram-negative bacteria play a crucial role in virulence and pathogenesis. identification of these proteins represents an important goal for bacterial proteomics, because it aids in vaccine development. here, we have developed such an approach for ehrlichia ruminantium, the obligate intracellular bacterium that causes heartwater. a preliminary whole proteome analysis of elementary bodies, the extracellular infectious form of the bacterium, had been performed ... | 2015 | 25710494 |
| survival after cryogenic freezing of campylobacter species in ground turkey patties treated with polyphosphates. | the use of polyphosphate-based marinades in the processing of poultry has been previously shown to increase the survival of campylobacter species present in the exudates derived from these products. this study investigates the effects that some of the same polyphosphates have on the survival of campylobacter species within a ground turkey product subjected to cryogenic freezing. ground turkey patties with two different polyphosphate formulations added in two different concentrations were artific ... | 2015 | 25710161 |
| environmental monitoring of waterborne campylobacter: evaluation of the australian standard and a hybrid extraction-free mpn-pcr method. | campylobacter is the leading agent of diarrheal disease worldwide. this study evaluates a novel culture-pcr hybrid (mpn-pcr) assay for the rapid enumeration of campylobacter spp. from estuarine and wastewater systems. to first evaluate the current, culture-based, australian standard, an inter-laboratory study was conducted on 69 subsampled water samples. the proposed most-probable number (mpn)-pcr method was then evaluated, by analysing 147 estuarine samples collected over a 2 year period. data ... | 2015 | 25709604 |
| concentration- and roughness-dependent antibacterial and antifungal activities of cuo thin films and their cu ion cytotoxicity and elution behavior. | in this study, we aimed to evaluate the antibacterial and antifungal properties, cytotoxicity, and elution behavior of copper oxide (cuo) thin films with varying concentrations and roughness values. cuo films greater than 0.2 mol % showed 99.9 % antimicrobial activity against escherichia coli, staphylococcus aureus, campylobacter jejuni, and penicillium funiculosum. cu ions were found to be noncytotoxic in new zealand white rabbits. the concentration of cu ions from cuo thin films eluted in drin ... | 2015 | 25708981 |
| purification of the campylobacter jejuni dps protein assisted by its high melting temperature. | dps proteins (dna binding protein from starved cell) form a distinct group within the ferritin superfamily. all dps members are composed of 12 identical subunits that assemble into a conserved spherical protein shell. dps oxidize fe(2+) in a conserved ferroxidase center located at the interface between monomers, the product of the reaction fe(3+), is then stored inside the protein shell in the form of non-reactive insoluble fe2o3. the campylobacter jejuni dps (cjdps) has been reported to play a ... | 2015 | 25707373 |
| infectious causes of cholesteatoma and treatment of infected ossicles prior to reimplantation by hydrostatic high-pressure inactivation. | chronic inflammation, which is caused by recurrent infections, is one of the factors contributing to the pathogenesis of cholesteatoma. if reimplantation of autologous ossicles after a surgical intervention is intended, inactivation of planktonic bacteria and biofilms is desirable. high hydrostatic pressure treatment is a procedure, which has been used to inactivate cholesteatoma cells on ossicles. here we discuss the potential inactivating effect of high hydrostatic pressure on microbial pathog ... | 2015 | 25705686 |
| fura contributes to the oxidative stress response regulation of mycobacterium avium ssp. paratuberculosis. | the ferric uptake regulator a (fura) is known to be involved in iron homeostasis and stress response in many bacteria. in mycobacteria the precise role of fura is still unclear. in the presented study, we addressed the functional role of fura in the ruminant pathogen mycobacterium avium ssp. paratuberculosis (map) by construction of a fura deletion strain (mapδfura). rna deep sequencing revealed that the fura regulon consists of repressed and activated genes associated to stress response or intr ... | 2015 | 25705205 |
| actinomyces-induced inflammatory myofibroblastic tumor of the colon: a rare cause of an abdominal mass: akbulut et al. inflammatory myofibroblastictumor due to actinomyces spp. | inflammatory myofibroblastic tumors (imfts) are neoplastic lesions that are either benign or have low-grade malignancy potential. although the etiopathogenesis is not entirely clear, many factors play a role in their development, including trauma, autoimmune disorders, and infectious and inflammatory processes. however, imfts caused by actinomyces spp. infection are rare, with a limited number of cases reported in the literature. | 2015 | 25704558 |
| bridging topological and functional information in protein interaction networks by short loops profiling. | protein-protein interaction networks (ppins) have been employed to identify potential novel interconnections between proteins as well as crucial cellular functions. in this study we identify fundamental principles of ppin topologies by analysing network motifs of short loops, which are small cyclic interactions of between 3 and 6 proteins. we compared 30 ppins with corresponding randomised null models and examined the occurrence of common biological functions in loops extracted from a cross-vali ... | 2015 | 25703051 |
| on the in vivo significance of bacterial resistance to antimicrobial peptides. | antimicrobial peptides (amps) are at the front-line of host defense during infection and play critical roles both in reducing the microbial load early during infection and in linking innate to adaptive immunity. however, successful pathogens have developed mechanisms to resist amps. although considerable progress has been made in elucidating amp-resistance mechanisms of pathogenic bacteria in vitro, less is known regarding the in vivo significance of such resistance. nevertheless, progress has b ... | 2015 | 25701234 |
| does the recent growth of aquaculture create antibiotic resistance threats different from those associated with land animal production in agriculture? | important antibiotics in human medicine have been used for many decades in animal agriculture for growth promotion and disease treatment. several publications have linked antibiotic resistance development and spread with animal production. aquaculture, the newest and fastest growing food production sector, may promote similar or new resistance mechanisms. this review of 650+ papers from diverse sources examines parallels and differences between land-based agriculture of swine, beef, and poultry ... | 2015 | 25700799 |
| predominant campylobacter jejuni sequence types persist in finnish chicken production. | consumption and handling of chicken meat are well-known risk factors for acquiring campylobacteriosis. this study aimed to describe the campylobacter jejuni population in finnish chickens and to investigate the distribution of c. jejuni genotypes on finnish chicken farms over a period of several years. we included 89.8% of the total c. jejuni population recovered in finnish poultry during 2004, 2006, 2007, 2008, and 2012 and used multilocus sequence typing (mlst) and pulsed-field gel electrophor ... | 2015 | 25700264 |
| lesser-known or hidden reservoirs of infection and implications for adequate prevention strategies: where to look and what to look for. | in developing hygiene strategies, in recent years, the major focus has been on the hands as the key route of infection transmission. however, there is a multitude of lesser-known and underestimated reservoirs for microorganisms which are the triggering sources and vehicles for outbreaks or sporadic cases of infection. among those are water reservoirs such as sink drains, fixtures, decorative water fountains and waste-water treatment plants, frequently touched textile surfaces such as private cur ... | 2015 | 25699227 |
| structural analysis of pseh, the campylobacter jejuni n-acetyltransferase involved in bacterial o-linked glycosylation. | campylobacter jejuni is a bacterium that uses flagella for motility and causes worldwide acute gastroenteritis in humans. the c. jejuni n-acetyltransferase pseh (cjpseh) is responsible for the third step in flagellin o-linked glycosylation and plays a key role in flagellar formation and motility. cjpseh transfers an acetyl group from an acetyl donor, acetyl coenzyme a (accoa), to the amino group of udp-4-amino-4,6-dideoxy-n-acetyl-β-l-altrosamine to produce udp-2,4-diacetamido-2,4,6-trideoxy-β-l ... | 2015 | 25698400 |
| analysis of the campylobacter jejuni genome by smrt dna sequencing identifies restriction-modification motifs. | campylobacter jejuni is a leading bacterial cause of human gastroenteritis. the goal of this study was to analyze the c. jejuni f38011 strain, recovered from an individual with severe enteritis, at a genomic and proteomic level to gain insight into microbial processes. the c. jejuni f38011 genome is comprised of 1,691,939 bp, with a mol.% (g+c) content of 30.5%. pacbio sequencing coupled with rebase analysis was used to predict c. jejuni f38011 genomic sites and enzymes that may be involved in d ... | 2015 | 25695747 |
| shelf life and storage stability of spray-dried bovine colostrum powders under different storage conditions. | spray dried bovine colostrum (sdbc) powders were packaged in aluminium-laminated polyethylene (alpe) and polyethylene terephthalate (pet) pouches and then stored under different conditions (25 °c and 50 % relative humidity (rh), 4 °c and 40-70 % rh, 50 °c and 20-50 % rh). the shelf life of sdbc powder was evaluated as 425.5 and 86.5 days in alpe and pet pouches under 25 °c and 50 % rh, respectively. the storage stability of sdbc powder in terms of quality parameters including thiobarbituric acid ... | 2013 | 25694704 |
| shelf life and storage stability of spray-dried bovine colostrum powders under different storage conditions. | spray dried bovine colostrum (sdbc) powders were packaged in aluminium-laminated polyethylene (alpe) and polyethylene terephthalate (pet) pouches and then stored under different conditions (25 °c and 50 % relative humidity (rh), 4 °c and 40-70 % rh, 50 °c and 20-50 % rh). the shelf life of sdbc powder was evaluated as 425.5 and 86.5 days in alpe and pet pouches under 25 °c and 50 % rh, respectively. the storage stability of sdbc powder in terms of quality parameters including thiobarbituric acid ... | 2013 | 25694704 |
| inhibition of adhesion of intestinal pathogens (escherichia coli, vibrio cholerae, campylobacter jejuni, and salmonella typhimurium) by common oligosaccharides. | inhibition of the binding of pathogenic adhesins to host glycans by suitable oligosaccharides forms the basis of antiadhesion therapies. experiments were carried out to study the inhibition capability of oligosaccharides on the adhesion of four microorganisms (escherichia coli, vibrio cholerae, campylobacter jejuni, and salmonella typhimurium) to ht-29 cells. results showed that, in the absence of oligosaccharides, all of the four pathogens efficiently adhered to the cells. cell adhesion with di ... | 2015 | 25692734 |
| analysis of the activity and regulon of the two-component regulatory system composed by cjj81176_1484 and cjj81176_1483 of campylobacter jejuni. | campylobacter jejuni is a leading cause of bacterial diarrheal disease and a frequent commensal of the intestinal tract in poultry and other animals. for optimal growth and colonization of hosts, c. jejuni employs two-component regulatory systems (tcss) to monitor environmental conditions and promote proper expression of specific genes. we analyzed the potential of c. jejuni cjj81176_1484 (cjj1484) and cjj81176_1483 (cjj1483) to encode proteins of a cognate tcs that influences expression of gene ... | 2015 | 25691530 |
| gut/brain axis and the microbiota. | tremendous progress has been made in characterizing the bidirectional interactions between the central nervous system, the enteric nervous system, and the gastrointestinal tract. a series of provocative preclinical studies have suggested a prominent role for the gut microbiota in these gut-brain interactions. based on studies using rodents raised in a germ-free environment, the gut microbiota appears to influence the development of emotional behavior, stress- and pain-modulation systems, and bra ... | 2015 | 25689247 |
| n-glycosylation with synthetic undecaprenyl pyrophosphate-linked oligosaccharide to oligopeptides by pglb oligosaccharyltransferase from campylobacter jejuni. | the oligosaccharyltransferase pglb from campylobacter jejuni catalyses the n-glycosylation reaction with undecaprenyl-pyrophosphate-linked glc1 galnac5 bac1 (und-pp-glc1 galnac5 bac1 ). experiments using chemically synthesized donors coupled to fluorescently tagged peptides confirmed that biosynthetic intermediate und-pp-bac1 and und-pp-galnac2 bac1 are transferred efficiently to the asn residue in the consensus sequence (d/e-x'-n-x-t/s, x',x≠p). the products were analyzed in detail by tandem ms ... | 2015 | 25688550 |
| unique atpase site architecture triggers cis-mediated synchronized atp binding in heptameric aaa+-atpase domain of flagellar regulatory protein flrc. | bacterial enhancer-binding proteins (bebps) oligomerize through aaa(+) domains and use atp hydrolysis-driven energy to isomerize the rna polymerase-σ(54) complex during transcriptional initiation. here, we describe the first structure of the central aaa(+) domain of the flagellar regulatory protein flrc (flrc(c)), a bebp that controls flagellar synthesis in vibrio cholerae. our results showed that flrc(c) forms heptamer both in nucleotide (nt)-free and -bound states without atp-dependent subunit ... | 2015 | 25688103 |
| specific genetic features of campylobacter jejuni strain g1 revealed by genome sequencing. | this report describes a draft genome sequence of campylobacter jejuni strain g1. the strain contains no plasmids, despite being resistant to tetracycline (tet). comparison of the genome of this strain with that of a reference strain nctc 11168 (tet sensitive) revealed the presence of a candidate gene that may be responsible for antibiotic resistance, as well as the genes involved in the synthesis of ganglioside-mimicking molecules responsible for the development of a neurodegenerative disorder, ... | 2015 | 25688069 |
| detection of campylobacter in human faecal samples in fiji. | data on campylobacteriosis in developed countries are well documented; in contrast, few studies on campylobacteriosis have been conducted in developing countries. this study was undertaken to test for campylobacter in human faecal samples sent to the two major pathology laboratories in fiji. | 2014 | 25685603 |
| antimicrobial resistance in campylobacter coli and campylobacter jejuni in cynomolgus monkeys (macaca fascicularis) and eradication regimens. | campylobacter spp. are zoonotic pathogens, however, knowledge about their presence and antimicrobial resistance in nonhuman primates is limited. our animal facility purchased cynomolgus monkeys (macaca fascicularis) from various asian countries: china, cambodia, indonesia, the philippines, and vietnam. | 2017 | 25683191 |
| significance of four methionine sulfoxide reductases in staphylococcus aureus. | staphylococcus aureus is a major human pathogen and emergence of antibiotic resistance in clinical staphylococcal isolates raises concerns about our ability to control these infections. cell wall-active antibiotics cause elevated synthesis of methionine sulfoxide reductases (msrs: msra1 and msrb) in s. aureus. msra and msrb enzymes reduce s-epimers and r-epimers of methionine sulfoxide, respectively, that are generated under oxidative stress. in the s. aureus chromosome, there are three msra gen ... | 2015 | 25680075 |
| clonalframeml: efficient inference of recombination in whole bacterial genomes. | recombination is an important evolutionary force in bacteria, but it remains challenging to reconstruct the imports that occurred in the ancestry of a genomic sample. here we present clonalframeml, which uses maximum likelihood inference to simultaneously detect recombination in bacterial genomes and account for it in phylogenetic reconstruction. clonalframeml can analyse hundreds of genomes in a matter of hours, and we demonstrate its usefulness on simulated and real datasets. we find evidence ... | 2015 | 25675341 |
| bacteremia caused by arcobacter butzleri in an immunocompromised host. | arcobacter butzleri is an emerging pathogen that has been implicated as the causative agent of persistent watery diarrhea. we describe a case involving a patient with chronic lymphocytic leukemia who developed invasive a. butzleri bacteremia. this case illustrates the unique challenges involved in diagnosing infections caused by emerging gastrointestinal pathogens. | 2015 | 25673792 |
| thiosulfate dehydrogenase (tsda) from allochromatium vinosum: structural and functional insights into thiosulfate oxidation. | although the oxidative condensation of two thiosulfate anions to tetrathionate constitutes a well documented and significant part of the natural sulfur cycle, little is known about the enzymes catalyzing this reaction. in the purple sulfur bacterium allochromatium vinosum, the reaction is catalyzed by the periplasmic diheme c-type cytochrome thiosulfate dehydrogenase (tsda). here, we report the crystal structure of the "as isolated" form of a. vinosum tsda to 1.98 å resolution and those of sever ... | 2015 | 25673691 |
| cloning, refolding, purification and preliminary crystallographic analysis of the sensory domain of the campylobacter chemoreceptor for multiple ligands (ccml). | a periplasmic sensory domain of the campylobacter jejuni chemoreceptor for multiple ligands (ccml) has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 as a precipitating agent. a complete data set was collected to 1.3 å resolution using cryocooling conditions and synchrotron radiation. the crystals belonged to space group p21, with unit-cell parameters a = 42.6, b = 138.0, c = 49.0 å, β = 94.3°. | 2015 | 25664798 |
| mechanisms of bacterial morphogenesis: evolutionary cell biology approaches provide new insights. | how darwin's "endless forms most beautiful" have evolved remains one of the most exciting questions in biology. the significant variety of bacterial shapes is most likely due to the specific advantages they confer with respect to the diverse environments they occupy. while our understanding of the mechanisms generating relatively simple shapes has improved tremendously in the last few years, the molecular mechanisms underlying the generation of complex shapes and the evolution of shape diversity ... | 2015 | 25664446 |
| a case of a laryngeal malt lymphoma in a patient with a history of gastric malt. | we are reporting a case of a 62-year-old african american woman with a history of gastric malt lymphoma successfully treated with radiation who presented with a laryngeal malt lymphoma 4 years after her original diagnosis. she received definitive radiation with a complete response. the case presented is unique for the rare presentation of a malt lymphoma in the larynx, especially in light of the patient's previously treated gastric malt lymphoma years ago. | 2015 | 25664189 |
| lactic acid bacteria as a surface display platform for campylobacter jejuni antigens. | food poisoning and diarrheal diseases continue to pose serious health care and socioeconomic problems worldwide. campylobacter spp. is a very widespread cause of gastroenteritis. over the past decade there has been increasing interest in the use of lactic acid bacteria (lab) as mucosal delivery vehicles. they represent an attractive opportunity for vaccination in addition to vaccination with attenuated bacterial pathogens. | 2015 | 25662187 |
| suicin 3908, a new lantibiotic produced by a strain of streptococcus suis serotype 2 isolated from a healthy carrier pig. | while streptococcus suis serotype 2 is known to cause severe infections in pigs, it can also be isolated from the tonsils of healthy animals that do not develop infections. we hypothesized that s. suis strains in healthy carrier pigs may have the ability to produce bacteriocins, which may contribute to preventing infections by pathogenic s. suis strains. two of ten s. suis serotype 2 strains isolated from healthy carrier pigs exhibited antibacterial activity against pathogenic s. suis isolates. ... | 2015 | 25659110 |
| peroxidase activity and involvement in the oxidative stress response of roseobacter denitrificans truncated hemoglobin. | roseobacter denitrificans is a member of the widespread marine roseobacter genus. we report the first characterization of a truncated hemoglobin from r. denitrificans (rd. trhb) that was purified in the heme-bound form from heterologous expression of the protein in escherichia coli. rd. trhb exhibits predominantly alpha-helical secondary structure and absorbs light at 412, 538 and 572 nm. the phylogenetic classification suggests that rd. trhb falls into group ii trhbs, whereas sequence alignment ... | 2015 | 25658318 |
| complete genome sequence of campylobacter jejuni yh001 from beef liver, which contains a novel plasmid. | campylobacter jejuni, commonly found in poultry and meat products, causes gastroenteritis in humans. here, we report the complete genome sequence of a c. jejuni strain, yh001, isolated from retail beef liver. the genome is 1,712,361 bp and has a 30.5% g+c content and two plasmids of 46.5 kb and 4.4 kb. | 2015 | 25657275 |
| specificity and evolutionary conservation of the escherichia coli rna pyrophosphohydrolase rpph. | bacterial rna degradation often begins with conversion of the 5'-terminal triphosphate to a monophosphate by the rna pyrophosphohydrolase rpph, an event that triggers rapid ribonucleolytic attack. besides its role as the master regulator of 5'-end-dependent mrna decay, rpph is important for the ability of pathogenic bacteria to invade host cells, yet little is known about how it chooses its targets. here, we show that escherichia coli rpph (ecrpph) requires at least two unpaired nucleotides at t ... | 2015 | 25657006 |
| identification of circulating biomarkers in sera of plasmodium knowlesi-infected malaria patients--comparison against plasmodium vivax infection. | plasmodium knowlesi was identified as the fifth major malaria parasite in humans. it presents severe clinical symptoms and leads to mortality as a result of hyperparasitemia in a short period of time. this study aimed to improve the current understanding of p. knowlesi and identify potential biomarkers for knowlesi malaria. | 2015 | 25656928 |
| management of the marginal zone lymphomas. | marginal zone lymphomas (mzl) represent around 8 % of all non-hodgkin lymphomas. during the last decades a number of studies have addressed the mechanisms underlying the disease development. extranodal mzl lymphoma usually arises in mucosal sites where lymphocytes are not normally present from a background of either autoimmune processes, such as hashimoto thyroiditis or sjögren syndrome or chronic infectious conditions. in the context of a persistent antigenic stimulation, successive genetic abn ... | 2015 | 25655612 |
| structural and functional implications of the interaction between macrolide antibiotics and bile acids. | macrolide antibiotics, such as azithromycin and erythromycin, are in widespread use for the treatment of bacterial infections. macrolides are taken up and excreted mainly by bile. additionally, they have been implicated in biliary system diseases and to modify the excretion of other drugs through bile. despite mounting evidence for the interplay between macrolide antibiotics and bile acids, the molecular details of this interaction remain unknown. herein, we show by nmr measurements that macroli ... | 2015 | 25655041 |
| validation of drop plate technique for bacterial enumeration by parametric and nonparametric tests. | drop plate technique has a priority and preference compared with the spread plate procedure, because of less time, quantity of media, effort requirement, little incubator space, and less labor intensive. the objective of this research was to compare the accuracy and fidelity of drop plate method vs. spread plate method by parametric and nonparametric statistical tests. for bacterial enumeration by drop and spread plate methods, successive dilutions of second subculture of lactobacillus casei and ... | 2013 | 25653794 |
| genome-wide gene order distances support clustering the gram-positive bacteria. | initially using 143 genomes, we developed a method for calculating the pair-wise distance between prokaryotic genomes using a monte carlo method to estimate the conservation of gene order. the method was based on repeatedly selecting five or six non-adjacent random orthologs from each of two genomes and determining if the chosen orthologs were in the same order. the raw distances were then corrected for gene order convergence using an adaptation of the jukes-cantor model, as well as using the co ... | 2015 | 25653643 |
| genome-wide gene order distances support clustering the gram-positive bacteria. | initially using 143 genomes, we developed a method for calculating the pair-wise distance between prokaryotic genomes using a monte carlo method to estimate the conservation of gene order. the method was based on repeatedly selecting five or six non-adjacent random orthologs from each of two genomes and determining if the chosen orthologs were in the same order. the raw distances were then corrected for gene order convergence using an adaptation of the jukes-cantor model, as well as using the co ... | 2015 | 25653643 |
| differential single nucleotide polymorphism-based analysis of an outbreak caused by salmonella enterica serovar manhattan reveals epidemiological details missed by standard pulsed-field gel electrophoresis. | we retrospectively analyzed a rare salmonella enterica serovar manhattan outbreak that occurred in italy in 2009 to evaluate the potential of new genomic tools based on differential single nucleotide polymorphism (snp) analysis in comparison with the gold standard genotyping method, pulsed-field gel electrophoresis. a total of 39 isolates were analyzed from patients (n=15) and food, feed, animal, and environmental sources (n=24), resulting in five different pulsed-field gel electrophoresis (pfge ... | 2015 | 25653407 |
| exploring the existence of lipid rafts in bacteria. | an interesting concept in the organization of cellular membranes is the proposed existence of lipid rafts. membranes of eukaryotic cells organize signal transduction proteins into membrane rafts or lipid rafts that are enriched in particular lipids such as cholesterol and are important for the correct functionality of diverse cellular processes. the assembly of lipid rafts in eukaryotes has been considered a fundamental step during the evolution of cellular complexity, suggesting that bacteria a ... | 2015 | 25652542 |
| dysbiosis of the gut microbiota in disease. | there is growing evidence that dysbiosis of the gut microbiota is associated with the pathogenesis of both intestinal and extra-intestinal disorders. intestinal disorders include inflammatory bowel disease, irritable bowel syndrome (ibs), and coeliac disease, while extra-intestinal disorders include allergy, asthma, metabolic syndrome, cardiovascular disease, and obesity. | 2015 | 25651997 |
| food-producing animals and their health in relation to human health. | the fields of immunology, microbiology, and nutrition converge in an astonishing way. dietary ingredients have a profound effect on the composition of the gut microflora, which in turn regulates the physiology of metazoans. as such, nutritional components of the diet are of critical importance not only for meeting the nutrient requirements of the host, but also for the microbiome. during their coevolution, bacterial microbiota has established multiple mechanisms to influence the eukaryotic host, ... | 2015 | 25651994 |
| enteropathogens detected in a daycare center, southeastern brazil: bacteria, virus, and parasite research. | the objective of this study was to determine the prevalence and etiological profile of enteropathogens in children from a daycare center. | 2015 | 25651323 |
| glycans and glycan-binding proteins in immune regulation: a concise introduction to glycobiology for the allergist. | cells are endowed with a rich surface coat of glycans that are carried as glycoproteins and glycolipids on the outer leaflets of their plasma membranes and constitute a major molecular interface between cells and their environment. each cell's glycome, the sum of its diverse glycan structures, comprises a distinct cellular signature defined by expression levels of the enzymes responsible for glycan biosynthesis. this signature can be read by complementary glycan-binding proteins (gbps) that tran ... | 2015 | 25649080 |
| differential rna-seq of vibrio cholerae identifies the vqmr small rna as a regulator of biofilm formation. | quorum sensing (qs) is a process of cell-to-cell communication that enables bacteria to transition between individual and collective lifestyles. qs controls virulence and biofilm formation in vibrio cholerae, the causative agent of cholera disease. differential rna sequencing (rna-seq) of wild-type v. cholerae and a locked low-cell-density qs-mutant strain identified 7,240 transcriptional start sites with ∼ 47% initiated in the antisense direction. a total of 107 of the transcripts do not appear ... | 2015 | 25646441 |
| faeco-prevalence of campylobacter jejuni in urban wild birds and pets in new zealand. | greater attention has been given to campylobacter jejuni (c. jejuni) prevalence in poultry and ruminants as they are regarded as the major contributing reservoirs of human campylobacteriosis. however, relatively little work has been done to assess the prevalence in urban wild birds and pets in new zealand, a country with the highest campylobacteriosis notification rates. therefore, the aim of the study was to assess the faeco-prevalence of c. jejuni in urban wild birds and pets and its temporal ... | 2015 | 25645429 |
| novel gentamicin resistance genes in campylobacter isolated from humans and retail meats in the usa. | to understand the molecular epidemiology of gentamicin-resistant campylobacter and investigate aminoglycoside resistance mechanisms. | 2015 | 25645207 |
| decline in rotavirus hospitalizations following the first three years of vaccination in castile-la mancha, spain. | rotavirus is a major burden on the spanish healthcare system. rotarix(®) and rotateq(®) were simultaneously commercialized in spain by february, 2007. the objective is to analyze the incidence and seasonality of rotavirus hospitalizations (rh) in castile-la mancha (clm), following the first 3 y of commercialization. a cross-sectional study of the hospital discharge registry's minimum basic data set (mbds) in clm between 2003 and 2009 was performed. we used the poisson regression model to quantif ... | 2015 | 25644531 |
| the flagellar regulator tvia reduces pyroptosis by salmonella enterica serovar typhi. | to discern virulent from innocuous microbes, the innate immune system senses events associated with bacterial access to immunoprivileged sites such as the host cell cytosol. one such pathway is triggered by the cytosolic delivery of flagellin, the major subunit of the flagellum, by bacterial secretion systems. this leads to inflammasome activation and subsequent proinflammatory cell death (pyroptosis) of the infected phagocyte. in this study, we demonstrate that the causative agent of typhoid fe ... | 2015 | 25644011 |
| aggregatibacter actinomycetemcomitans cytolethal distending toxin activates the nlrp3 inflammasome in human macrophages, leading to the release of proinflammatory cytokines. | the cytolethal distending toxin (cdt) is produced from a number of bacteria capable of causing infection and inflammatory disease. our previous studies with actinobacillus actinomycetemcomitans cdt demonstrate not only that the active toxin subunit functions as a phosphatidylinositol-3,4,5-triphosphate (pip3) phosphatase but also that macrophages exposed to the toxin were stimulated to produce proinflammatory cytokines. we now demonstrate that the cdt-induced proinflammatory response involves th ... | 2015 | 25644004 |
| the escherichia coli o157:h7 cattle immunoproteome includes outer membrane protein a (ompa), a modulator of adherence to bovine rectoanal junction squamous epithelial (rse) cells. | building on previous studies, we defined the repertoire of proteins comprising the immunoproteome (ip) of escherichia coli o157:h7 (o157) cultured in dmem supplemented with norepinephrine (o157 ip), a β-adrenergic hormone that regulates e. coli o157 gene expression in the gastrointestinal tract, using a variation of a novel proteomics-based platform proteome mining tool for antigen discovery, called "proteomics-based expression library screening" (pels; kudva et al., 2006). the e. coli o157 ip ( ... | 2015 | 25643951 |
| comparative study of the effects of citral on the growth and injury of listeria innocua and listeria monocytogenes cells. | this study investigates the effect of citral on growth and on the occurrence of sublethal damage in listeria innocua serovar 6a (cect 910) and listeria monocytogenes serovar 4b (cect 4032) cells that were exposed to citral as a natural antimicrobial agent. two initial inoculum concentrations were considered in this investigation: 10(2) and 10(6) cfu/ml. citral exhibited antilisterial activity against l. innocua and l. monocytogenes, and the observed effects were dependent on the concentration of ... | 2015 | 25643164 |
| a comparative analysis of methylome profiles of campylobacter jejuni sheep abortion isolate and gastroenteric strains using pacbio data. | campylobacter jejuni is a leading cause of human gastrointestinal disease and small ruminant abortions in the united states. the recent emergence of a highly virulent, tetracycline-resistant c. jejuni subsp. jejuni sheep abortion clone (clone sa) in the united states, and that strain's association with human disease, has resulted in a heightened awareness of the zoonotic potential of this organism. pacific biosciences' single molecule, real-time sequencing technology was used to explore the vari ... | 2014 | 25642218 |
| a comparative analysis of methylome profiles of campylobacter jejuni sheep abortion isolate and gastroenteric strains using pacbio data. | campylobacter jejuni is a leading cause of human gastrointestinal disease and small ruminant abortions in the united states. the recent emergence of a highly virulent, tetracycline-resistant c. jejuni subsp. jejuni sheep abortion clone (clone sa) in the united states, and that strain's association with human disease, has resulted in a heightened awareness of the zoonotic potential of this organism. pacific biosciences' single molecule, real-time sequencing technology was used to explore the vari ... | 2014 | 25642218 |
| mammalian gastrointestinal tract parameters modulating the integrity, surface properties, and absorption of food-relevant nanomaterials. | many natural chemicals in food are in the nanometer size range, and the selective uptake of nutrients with nanoscale dimensions by the gastrointestinal (gi) tract is a normal physiological process. novel engineered nanomaterials (nms) can bring various benefits to food, e.g., enhancing nutrition. assessing potential risks requires an understanding of the stability of these entities in the gi lumen, and an understanding of whether or not they can be absorbed and thus become systemically available ... | 2015 | 25641962 |
| a proposed essential gene discovery pipeline: a campylobacter jejuni case study. | genes required for an organism's growth and survival are termed essential and represent potential intervention targets. following in the footsteps of the genomics era, the "next-gen" genomic era provides vast amounts of genetic information. sequencing of a representative bacterial pathogen genome has been superseded by sequencing of whole strain collections, whether from environmental or clinical sources (harris et al., science 327:469-474, 2010; lewis et al., j hosp infect 75:37-41, 2010; beres ... | 2015 | 25636619 |
| microarray transposon tracking for the mapping of conditionally essential genes in campylobacter jejuni. | although whole genome approaches to the study of bacteria have grown substantially in the past decade, there is still a need for quick and easy methods for the determination of which genes are essential for the growth of these bacteria under specific growth conditions. there are numerous methods to accomplish this depending on the resources and equipment available, each with their own advantages and disadvantages. here we describe a method we successfully employed to map the essential genes of c ... | 2015 | 25636609 |
| multidrug efflux pumps from enterobacteriaceae, vibrio cholerae and staphylococcus aureus bacterial food pathogens. | foodborne illnesses caused by bacterial microorganisms are common worldwide and constitute a serious public health concern. in particular, microorganisms belonging to the enterobacteriaceae and vibrionaceae families of gram-negative bacteria, and to the staphylococcus genus of gram-positive bacteria are important causative agents of food poisoning and infection in the gastrointestinal tract of humans. recently, variants of these bacteria have developed resistance to medically important chemother ... | 2015 | 25635914 |
| direct detection and quantification of bacterial genes associated with inflammation in dna isolated from stool. | although predominantly associated with health benefits, the gut microbiota has also been shown to harbor genes that promote inflammation. in this work, we report a method for the direct detection and quantification of these pro-inflammatory bacterial genes by pcr and qpcr in dna extracted from human stool samples. pcr reactions were performed to detect (i) the pks island genes, (ii) tcpc, which is present in some strains of escherichia coli and (iii) gele presented in some strains of enterococcu ... | 2014 | 25635239 |
| rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations. | the incidence of foodborne diseases has increased over the years and resulted in major public health problem globally. foodborne pathogens can be found in various foods and it is important to detect foodborne pathogens to provide safe food supply and to prevent foodborne diseases. the conventional methods used to detect foodborne pathogen are time consuming and laborious. hence, a variety of methods have been developed for rapid detection of foodborne pathogens as it is required in many food ana ... | 2014 | 25628612 |
| rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations. | the incidence of foodborne diseases has increased over the years and resulted in major public health problem globally. foodborne pathogens can be found in various foods and it is important to detect foodborne pathogens to provide safe food supply and to prevent foodborne diseases. the conventional methods used to detect foodborne pathogen are time consuming and laborious. hence, a variety of methods have been developed for rapid detection of foodborne pathogens as it is required in many food ana ... | 2014 | 25628612 |
| using geovisual analytics in google earth to understand disease distribution: a case study of campylobacteriosis in the czech republic (2008-2012). | visual analytics aims to connect the processing power of information technologies and the user's ability of logical thinking and reasoning through the complex visual interaction. moreover, the most of the data contain the spatial component. therefore, the need for geovisual tools and methods arises. either one can develop own system but the dissemination of findings and its usability might be problematic or the widespread and well-known platform can be utilized. the aim of this paper is to prove ... | 2015 | 25628063 |
| the susceptibility of campylobacter concisus to the bactericidal effects of normal human serum. | campylobacter concisus is an emerging pathogen of the gastrointestinal tract that has been associated with barrett's oesophagus, enteritis and inflammatory bowel disease. despite having invasive potential in intestinal epithelial cells in-vitro, bacteraemic cases with c. concisus are extremely scarce, having only been reported once. therefore, we conducted a serum resistance assay to investigate the bactericidal effects of human complement on c. concisus in comparison to some other campylobacter ... | 2015 | 25627875 |
| the antimicrobial effect of spice-based marinades against campylobacter jejuni on contaminated fresh broiler wings. | the antimicrobial effect of spice-based marinades against campylobacter jejuni on inoculated fresh broiler wings was investigated. experiments were carried out with 1 strain of c. jejuni and 6 marinades. four experimental marinades were composed for the study and contained spices (thyme, rosemary, basil, marjoram, and so on) and different combination of bioactive compounds. two marinades were commercial and contained spices (black pepper, sweet red pepper, and so on) and chemical additives (mono ... | 2015 | 25627752 |
| network representations of immune system complexity. | the mammalian immune system is a dynamic multiscale system composed of a hierarchically organized set of molecular, cellular, and organismal networks that act in concert to promote effective host defense. these networks range from those involving gene regulatory and protein-protein interactions underlying intracellular signaling pathways and single-cell responses to increasingly complex networks of in vivo cellular interaction, positioning, and migration that determine the overall immune respons ... | 2015 | 25625853 |
| microbiota that affect risk for shigellosis in children in low-income countries. | pathogens in the gastrointestinal tract exist within a vast population of microbes. we examined associations between pathogens and composition of gut microbiota as they relate to shigella spp./enteroinvasive escherichia coli infection. we analyzed 3,035 stool specimens (1,735 nondiarrheal and 1,300 moderate-to-severe diarrheal) from the global enteric multicenter study for 9 enteropathogens. diarrheal specimens had a higher number of enteropathogens (diarrheal mean 1.4, nondiarrheal mean 0.95; p ... | 2015 | 25625766 |
| simulation study of the effect of influenza and influenza vaccination on risk of acquiring guillain-barré syndrome. | it is unclear whether seasonal influenza vaccination results in a net increase or decrease in the risk for guillain-barré syndrome (gbs). to assess the effect of seasonal influenza vaccination on the absolute risk of acquiring gbs, we used simulation models and published estimates of age- and sex-specific risks for gbs, influenza incidence, and vaccine effectiveness. for a hypothetical 45-year-old woman and 75-year-old man, excess gbs risk for influenza vaccination versus no vaccination was -0.3 ... | 2015 | 25625590 |
| mechanisms of distal axonal degeneration in peripheral neuropathies. | peripheral neuropathy is a common complication of a variety of diseases and treatments, including diabetes, cancer chemotherapy, and infectious causes (hiv, hepatitis c, and campylobacter jejuni). despite the fundamental difference between these insults, peripheral neuropathy develops as a combination of just six primary mechanisms: altered metabolism, covalent modification, altered organelle function and reactive oxygen species formation, altered intracellular and inflammatory signaling, slowed ... | 2015 | 25617478 |
| retraction for dinant et al., aortic homograft endocarditis caused by campylobacter jejuni. | 2015 | 25617446 | |
| cloning, expression, refolding, purification and preliminary crystallographic analysis of the sensory domain of the campylobacter chemoreceptor for aspartate a (ccaa). | in campylobacter jejuni, chemotaxis and motility have been identified as important virulence factors that are required for host colonization and invasion. chemotactic recognition of extracellular signals is mediated by the periplasmic sensory domains of its transducer-like proteins (tlps). in this study, the sensory domain of the c. jejuni chemoreceptor for aspartate a (ccaa) has been expressed in escherichia coli and purified from inclusion bodies. the urea-denatured protein was refolded and th ... | 2015 | 25615981 |
| a sequential statistical approach towards an optimized production of a broad spectrum bacteriocin substance from a soil bacterium bacillus sp. yas 1 strain. | bacteriocins, ribosomally synthesized antimicrobial peptides, display potential applications in agriculture, medicine, and industry. the present study highlights integral statistical optimization and partial characterization of a bacteriocin substance from a soil bacterium taxonomically affiliated as bacillus sp. yas 1 after biochemical and molecular identifications. a sequential statistical approach (plackett-burman and box-behnken) was employed to optimize bacteriocin (bac yas 1) production. u ... | 2014 | 25614886 |
| role of cytokines and toll-like receptors in the immunopathogenesis of guillain-barré syndrome. | guillain-barré syndrome (gbs) is an autoimmune disease of the peripheral nervous system, mostly triggered by an aberrant immune response to an infectious pathogen. although several infections have been implicated in the pathogenesis of gbs, not all such infected individuals develop this disease. moreover, infection with a single agent might also lead to different subtypes of gbs emphasizing the role of host factors in the development of gbs. the host factors regulate a broad range of inflammator ... | 2014 | 25614713 |
| in vivo production of a novel glycoconjugate vaccine against shigella flexneri 2a in recombinant escherichia coli: identification of stimulating factors for in vivo glycosylation. | glycoconjugated vaccines composed of polysaccharide antigens covalently linked to immunogenic carrier proteins have proved to belong to the most effective and safest vaccines for combating bacterial pathogens. the functional transfer of the n-glycosylation machinery from campylobacter jejuni to the standard prokaryotic host escherichia coli established a novel bioconjugation methodology termed bacterial glycoengineering. | 2015 | 25612741 |