Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| absence of an endogenous regulator of na+,k+-atpase activity in the tissues of cirrhotic rats. | microsomal na+,k+-atpase isolated from the renal cortex of rats with ccl4-induced cirrhosis (cir) showed a higher specific activity than the enzyme obtained from control rats (cor). kinetic studies showed a lower k0.5 for atp (0.08 +/- 0.03 vs. 0.24 +/- 0.04 mm; p less than 0.05), a lower na+ activation constant (9.6 +/- 1.5 vs. 19.0 +/- 1.7 mm; p less than 0.05), and a higher k+ activation constant (1.2 +/- 0.1 vs. 0.6 +/- 0.1 mm; p less than 0.05) for cir. the optimal ph of the enzyme was 0.5 ... | 1988 | 2838057 |
| mechanism of the epsp synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis. | in order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (epsp) synthase reaction, we have investigated the interaction of epsp synthase with the reaction product epsp. an exchange of epsp-methylene protons could be demonstrated by incubating epsps with epsp in d2o. since trace amounts of contaminating pi would lead to reversal of epsps reaction and hence methylene proton exchange, we added pyruvate kinase, adp, mg++ and k+. under these conditions, any contami ... | 1988 | 2838023 |
| immune response of chickens inoculated with a recombinant avian leukosis virus. | specific-pathogen-free embryos (18-day incubation) and hatched chicks were inoculated with a recombinant avian leukosis virus (alv) produced by recombinant dna techniques. enzyme-linked immunosorbent assays were used to measure the production of viral-protein-specific antibody and the viral protein, p27, in the serum at 2, 5, 8, 14, and 20 weeks of age. of the inoculated chickens surviving to 20 weeks, 64% produced viral-protein-specific antibodies and 42% transiently produced the viral protein, ... | 1988 | 2838018 |
| insulinoma. the value of intraoperative ultrasonography. | after establishing the diagnosis of an insulinoma, most surgeons prefer preoperative localization. selective arteriography has long remained the gold standard for this purpose, but its use has been met with variable success. despite various attempts at localization, insulinomas remain undetected in 10% to 20% of patients, and there may be a postoperative complication rate of at least 10% to 25%. to review the results and surgical implications of current localization techniques, 36 adult patients ... | 2010 | 2838008 |
| immune regulation by leukotriene b4. | 2010 | 2837957 | |
| treatment of cytomegalovirus infections in renal transplant recipients with 9-(1,3-dihydroxy-2-propoxymethyl) guanine. | 1988 | 2837848 | |
| [significance and practice of recall/after care]. | 2009 | 2836949 | |
| adp-ribosylation by cholera toxin of membranes derived from brain modifies the interaction of adenylate cyclase with guanine nucleotides and naf. | we have developed a method to adp-ribosylate the stimulatory guanine nucleotide-binding protein of adenylate cyclase (gs) in brain membranes by using cholera toxin. in particular, we used isonicotinic acid hydrazide and 3-acetylpyridine adenine dinucleotide to inhibit the potent nad-glycohydrolase activity of brain membranes, and we used the detergent triton x-100 (at 0.1%) to improve the accessibility of the toxin and guanine nucleotides used for supporting the adp-ribosylation. this method rev ... | 1988 | 2836559 |
| adp-ribosylation by cholera toxin of membranes derived from brain modifies the interaction of adenylate cyclase with guanine nucleotides and naf. | we have developed a method to adp-ribosylate the stimulatory guanine nucleotide-binding protein of adenylate cyclase (gs) in brain membranes by using cholera toxin. in particular, we used isonicotinic acid hydrazide and 3-acetylpyridine adenine dinucleotide to inhibit the potent nad-glycohydrolase activity of brain membranes, and we used the detergent triton x-100 (at 0.1%) to improve the accessibility of the toxin and guanine nucleotides used for supporting the adp-ribosylation. this method rev ... | 1988 | 2836559 |
| a novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in vibrio cholerae requires toxr. | the toxr gene of vibrio cholerae encodes a transmembrane, dna-binding protein that activates transcription of the cholera toxin operon and a gene (tcpa) for the major subunit of a pilus colonization factor. we constructed site-directed insertion mutations in the toxr gene by a novel method employing the chromosomal integration of a mobilizable suicide plasmid containing a portion of the toxr coding sequence. mutants containing these new toxr alleles had an altered outer membrane protein profile, ... | 1988 | 2836362 |
| initial clinical experience with degradable starch microspheres. | four patients with advanced hepatic malignancy have been investigated with a view to intra-arterial treatment with degradable starch microspheres (dsm) and mitomycin-c (mmc). computer-assisted interpretation of data gathered during the administration of technetium-labelled albumin allows the degree of arteriovenous shunting within the liver to be measured. in a similar way, the effectiveness of dsm in blocking hepatic arterial flow is determined. two patients showed unacceptably high levels of s ... | 2009 | 2835715 |
| initial clinical experience with degradable starch microspheres. | four patients with advanced hepatic malignancy have been investigated with a view to intra-arterial treatment with degradable starch microspheres (dsm) and mitomycin-c (mmc). computer-assisted interpretation of data gathered during the administration of technetium-labelled albumin allows the degree of arteriovenous shunting within the liver to be measured. in a similar way, the effectiveness of dsm in blocking hepatic arterial flow is determined. two patients showed unacceptably high levels of s ... | 2009 | 2835715 |
| [a new inflammatory muscular disease: inclusion-body myositis]. | 2009 | 2835126 | |
| reversal by cyclic amp of the urea-induced inhibition of synthesis of a catabolite-repressible enzyme in vibrio cholerae. | low concentrations of urea, which did not inhibit the synthesis of the catabolite nonrepressible enzyme alkaline phosphatase in vibrio cholerae, or markedly affect its overall growth, specifically inhibited the expression of the tryptophanase operon in a temperature-dependent manner. however, in contrast to what is found in escherichia coli, this urea-induced inhibition of tryptophanase synthesis in v. cholerae could be almost completely relieved by exogenously added cyclic amp. the possible mec ... | 1987 | 2833565 |
| effects of spiraprilic acid, an angiotensin converting enzyme inhibitor, on large artery compliance in anesthetized dogs. | the present study examined the actions of spiraprilic acid, a new non-sulfhydryl angiotensin converting enzyme (ace) inhibitor upon aortic compliance (ac) in anesthetized dogs. enalaprilic acid was examined for comparative purposes. ac was determined via sonomicrometric determination of the ratio of aortic systolic-diastolic diameter (mm) and arterial pulse pressure (mmhg). one ac unit (acu) equals 10(-3) mm/mmhg. in non-thiazide pretreated animals, spiraprilic acid (1 mg/kg i.v.), caused a sust ... | 1987 | 2833190 |
| isolation of a simian virus 40-simian agent 12 recombinant papovavirus that synthesizes a hybrid large tumour antigen. | the simian virus 40 (sv40) mutant dl1055 carries a 55 base pair deletion in the viral early region coding sequences that causes premature termination of large tumour (t) antigen translation resulting in a protein fragment consisting of the amino-terminal 399 residues. the mutation renders the virus defective. we report the characterization of hyb4001, which was isolated as a single plaque following cotransfection of permissive cells with dl1055 dna and the dna of a related papovavirus, simian ag ... | 1988 | 2832530 |
| glucose and carbachol generate 1,2-diacylglycerols by different mechanisms in pancreatic islets. | diacylglycerols (dag) modulate secretory responses by the activation of protein kinase c. early changes in dag formation induced by the muscarinic receptor agonist carbachol were compared to those caused by the nutrient secretagogue glucose in pancreatic islets. turnover rates of dag were investigated in radiolabeling experiments, whereas changes in total mass and fatty acid composition of dag were assessed by gas-liquid chromatography. when islet lipids were labeled to steady state in tissue cu ... | 1988 | 2832445 |
| rapid expression of ornithine decarboxylase mrna in a macrophage-like cell line: camp repression of the requirement for prior protein synthesis. | ornithine decarboxylase (odc) activity was rapidly induced in the raw264 macrophage-like cell line after treatment with bacterial lipopolysaccharide (lps). odc mrna levels were determined by isolating cellular rna, followed by northern blot and dot blot analysis using a 32p-labeled cdna probe. odc mrna levels increased within 1 hour of stimulation of raw264 cells with 1.0 microgram/ml lps. transcription rate analysis on isolated nuclei indicated that an increase in transcription rate contributed ... | 2010 | 2832424 |
| characterization and mapping of the agrocinopine-agrocin 84 locus on the nopaline ti plasmid ptic58. | overlapping segments of ptic58 inserted into cosmid vectors were used to characterize the agrocinopine-agrocin 84 locus from the nopaline/agrocinopine a and b agrobacterium tumefaciens strain c58. all of the clones conferring agrocin 84 sensitivity on agrobacteria also conferred uptake of agrocin 84 and agrocinopines a and b. transposon tn3-hoho1 insertion mutations of one such clone were generated that simultaneously abolished agrocin 84 sensitivity and transport of agrocinopines a and b and ag ... | 1988 | 2832379 |
| cloning and expression of the vibrio cholerae neuraminidase gene nanh in escherichia coli. | a cosmid gene bank of vibrio cholerae 395, classical ogawa, was screened in escherichia coli hb101 for expression of the vibrio neuraminidase (nanase) gene nanh (n-acylneuraminate glycohydrolase). positive clones were identified by their ability to cleave the fluorogenic nanase substrate 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid. seven nanase-positive clones were detected after screening 683 cosmid isolates with a rapid, qualitative plate assay method. the nanh gene was subcloned ... | 1988 | 2832365 |
| nmr contrast agents for proton imaging. | 2010 | 2832350 | |
| effect of cyclosporin a immunosuppression on primary lymphotropic herpesvirus infection in the guinea pig. | female guinea pigs pretreated for 2 days with cyclosporin a (csa), were then inoculated intranasally (in) or intraperitoneally (ip) with a lymphotropic herpesvirus (gphlv) and followed by 4 additional daily doses of csa. immunosuppressed animals did not show lymphocytosis and virus infectivity titers in their spleen, cervical lymph node and blood mononuclear cells were lower than those of oil-treated controls. while in-inoculated csa-treated animals expressed higher virus infectivity titer in th ... | 1987 | 2832345 |
| hot nodule after fine needle aspiration. | 2009 | 2832117 | |
| non-cholinergic basal forebrain neurons project to the contralateral basal forebrain in the rat. | following injections of wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp) or the fluorescent tracer fluoro-gold into the magnocellular preoptic area and the horizontal limb of the diagonal band, retrogradely labelled neurons were found in the homotopic region of the contralateral basal forebrain. labelled fibers apparently arising from these neurons travelled in the stria medullaris and the habenular commissure to terminate in the contralateral basal forebrain. although the neuro ... | 1988 | 2831483 |
| a possible pain control system in a non-mammalian vertebrate (a lizard, gekko gecko). | in a lizard (gekko gecko) the anterograde tracer pha-l was microiontophoretically applied to the predominantly serotonergic nucleus raphes inferior. extensive spinal projections from the rostral magnocellular part of this nucleus were demonstrated to the superficial layers of the dorsal horn and to the intermediate zone, more sparsely to the ventral horn. but, in addition, retrogradely labeled neurons were found in and just below a periventricular cell group in tegmentum mesencephali, i.e. the l ... | 2010 | 2831478 |
| decreased surfactant level in the lung with oligohydramnios: a morphometric and biochemical study. | 2009 | 2831329 | |
| decreased surfactant level in the lung with oligohydramnios: a morphometric and biochemical study. | 2009 | 2831329 | |
| kirsten murine sarcoma virus abolishes interferon gamma-induced class ii but not class i major histocompatibility antigen expression in a murine fibroblast line. | the effect of infecting fibroblasts with kirsten murine sarcoma virus/murine leukemia virus (ki-msv/mlv) on constitutive and ifn-gamma-induced h-2 antigen expression was investigated. the fibroblasts used were two established cell lines (c3h10t1/2 and balb/c3t3) and fresh embryo fibroblasts from c3h mice. class i antigens were expressed constitutively by balb/c3t3; infection with mlv, msv or the two together had little effect on this constitutive expression. class i antigens (h-2k, h-2d) were st ... | 1988 | 2831293 |
| [genetic transformation of somatic cells. xv. production of transgenic mice containing the gene for the envelope protein of the human hepatitis b virus]. | transgenic mice containing in somatic (liver) cells a gene encoding hepatitis virus b surface antigen (hbsag) were obtained and analysed. about 10(4)-10(5) copies of circular plasmid psh21 with hbsag gene under the control of the sv40 early promoter were microinjected into the cytoplasm (pronucleus region) of mouse zygotes. 10 of the 24 animals isolated after this treatment contained 1 to 5 copies of the hbsag gene per genome of hepatocytes (7 males and 3 females). the southern analysis of dna f ... | 1987 | 2830691 |
| [genetic transformation of somatic cells. xv. production of transgenic mice containing the gene for the envelope protein of the human hepatitis b virus]. | transgenic mice containing in somatic (liver) cells a gene encoding hepatitis virus b surface antigen (hbsag) were obtained and analysed. about 10(4)-10(5) copies of circular plasmid psh21 with hbsag gene under the control of the sv40 early promoter were microinjected into the cytoplasm (pronucleus region) of mouse zygotes. 10 of the 24 animals isolated after this treatment contained 1 to 5 copies of the hbsag gene per genome of hepatocytes (7 males and 3 females). the southern analysis of dna f ... | 1987 | 2830691 |
| [visualization of an ischiadic hernia using peritoneography]. | 2010 | 2829318 | |
| investigation of opioid receptor binding protein in adult and fetal mouse brain membranes by photoaffinity labeling. | 125i-[d-ala2,p-n3-phe4-met5]enkephalin was used to investigate the opioid receptor from brain membranes of adult (6 months old) and fetal (16 days gestation) mice. the photolabeled membranes were analyzed by sodium dodecyl sulfate gel electrophoresis. a 46,000-da protein was specifically photo-labeled in the adult mouse brain membranes. the photolabeling of this protein was inhibited in the presence of [d-ala2,met5]enkephalin and a peptide ligand specific for the mu-opioid receptor. no specific ... | 1988 | 2829063 |
| interaction between highly selective mu and delta opioids in vivo at the rat spinal cord. | possible interactions between mu- and delta-receptors in the rat spinal cord were studied using the radiant-heat-induced tail flick response and the highly selective mu- or delta-ligands: [nmephe3,d-pro4] morphiceptin(pl-17) and cyclic[d-pen2,d-pen5]enkephalin(dpdpe). intrathecal infusions of pl-17 (0.5 microgram/hr) for 5 days caused 61- and 6-fold shifts to the right of the dose-response curves for pl-17 and dpdpe, respectively. since pl-17 and dpdpe are highly selective agonists for mu- and d ... | 1986 | 2829017 |
| differential effects of benzodiazepines on phospholipid methylation in hippocampus and cerebellum of rats. | to elucidate the relationship between the occupancy of bdz binding sites and phospholipid methylation in brain, we examined phosphatidylethanolamine-n-methyltransferase (pemt) activity in synaptosomes of rat hippocampi and cerebella in the presence of bdz ligands with different modes of action. we found that ro 5-4864, a specific ligand for "peripheral type" receptors, increased pl methylation in hippocampal and cerebellar synaptosomes. this effect was directly related to receptor occupancy, sin ... | 1988 | 2828796 |
| inhibition of rat brain adenylate cyclase activity by benzodiazepine through the effects on gi and catalytic proteins. | the effect of benzodiazepines on adenylate cyclase system was examined in rat brain. micromolar concentrations of diazepam inhibited the enzyme activity in synaptic membranes in dose- and time-dependent manners. the inhibitory effect of diazepam was more evident on the enzyme activity in the presence of guanylyl-5'-imidodiphosphate (gppnhp) or naf-alcl3 than on that in the basal state. in the pertussis toxin-treated membranes, the effect of diazepam in the presence of gppnhp or naf-alcl3 was mar ... | 1988 | 2828793 |
| a comparison of pcp-like compounds for nmda antagonism in two in vivo models. | the pcp-like compounds ketamine and dexoxadrol were evaluated in two behavioral test procedures known to be sensitive to competitive n-methyl-d-aspartate (nmda) receptor antagonists. in the nmda-induced convulsion test in mice, ketamine and dexoxadrol blocked convulsant activity only at doses that also induced nonspecific effects of pcp-like behaviors, thereby confounding the interpretation of results. these compounds also blocked nmda-induced discriminative stimuli in rats; however, this effect ... | 1988 | 2828792 |
| the preparation of a carbon-11 labelled neurohormone--[11c]melatonin. | melatonin, a hormone of the pineal gland, has been labelled with the positron-emitting radionuclide, carbon-11 (t1/2 = 20.4 min), for study in vivo by positron emission tomography. thus a novel labelling agent, [11c]acetyl chloride, was prepared by the carbonation of methylmagnesium bromide with cyclotron-produced [11c]carbon dioxide followed by treatment with phthaloyl dichloride. acylation of 5-methoxytryptamine then affords [11c]melatonin, which is purified by hplc and formulated for intraven ... | 2010 | 2828278 |
| lipid-dependent membrane enzymes. purification to homogeneity and further characterization of diacylglycerol kinase from escherichia coli. | 1. diacylglycerol kinase apoprotein was purified from membranes of escherichia coli k12 by a six-step procedure that included hplc. the proposed assignment of the enzyme to the dgka gene [lightner et al. (1983) j. biol. chem. 258, 10856-10861] could be supported by molecular mass determination (approximately 14 kda), n-terminal sequencing (met-ala-asn), cyanogen bromide fragmentation and amino acid analysis. as predicted, proline was absent. 2. the membrane-associated as well as the butan-1-ol-d ... | 1988 | 2828054 |
| l929 cells infected with temperature sensitive mutants of vesicular stomatitis virus: virus replication is necessary for induction of changes in membrane permeability. | infection of l929 murine cells with vesicular stomatitis virus (vsv) results in inhibition of host protein synthesis and appearance of membrane alterations at a time when cells are still actively engaged in viral protein synthesis. vsv temperature-sensitive (ts) mutants have been used to explore the role(s) played by the virus-coded proteins in the genesis of these effects. cells were infected with each of five ts mutants representing the known complementation groups of vsv indiana serotype, and ... | 1987 | 2827608 |
| l929 cells infected with temperature sensitive mutants of vesicular stomatitis virus: virus replication is necessary for induction of changes in membrane permeability. | infection of l929 murine cells with vesicular stomatitis virus (vsv) results in inhibition of host protein synthesis and appearance of membrane alterations at a time when cells are still actively engaged in viral protein synthesis. vsv temperature-sensitive (ts) mutants have been used to explore the role(s) played by the virus-coded proteins in the genesis of these effects. cells were infected with each of five ts mutants representing the known complementation groups of vsv indiana serotype, and ... | 1987 | 2827608 |
| pain reduction in local anesthetic administration through ph buffering. | the effects of ph buffering on the pain of administration and efficacy of three local anesthetics (1% lidocaine, 1% lidocaine with 1:100,000 epinephrine, and 1% mepivacaine) were investigated in a randomized, prospective, double-blind study of 25 adult volunteers. plain and buffered solutions of the three local anesthetics were prepared, and a 0.5 intradermal injection of each was administered. pain of anesthetic infiltration was rated from zero to ten. the area of anesthetized skin surrounding ... | 2010 | 2827545 |
| herpes simplex virus infection of the hand. a profile of 79 cases. | seventy-nine cases of herpes simplex (hsv) infection of the hand were documented by viral culture over a 40-month period. a marked bimodal age distribution was found with 32 cases occurring in adults aged 21 to 30 years and 16 occurring in children less than 10 years old. the infection most commonly occurred on the fingers (69 percent) and thumb (21 percent). all 20 herpes simplex type 2 (hsv-2) isolates came from patients over 20 years old. all of the 13 specimens that were typed after isolatio ... | 1988 | 2827469 |
| mucosal antibody response of calves after oral and intrabronchial administration of rotavirus. | 1987 | 2827448 | |
| derivation of a monoclonal antibody against the group specific antigen of rotaviruses and its use in a diagnostic enzymatic immunoassay. | 1986 | 2827408 | |
| [classification of spot-shaped lung changes by texture analysis]. | classification of opacities in pneumoconiosis was accomplished by textural analysis in digitised chest x-rays. a good discrimination was achieved by a set of 10 parameters. these texture measures were computed by algorithms for edge detection, local extremes, difference statistics, the co-occurrence matrix and the power spectrum. the classes of the training set were classified correctly at 99%. a test set comprising additional classes that were not contained in the training set, was classified a ... | 1987 | 2827257 |
| characterization and restriction analysis of the p sex factor and the cryptic plasmid of vibrio cholerae strain v58. | the p plasmid of vibrio cholerae is a derepressed sex factor restricted to v. cholerae and has been shown to express surface exclusion. we have isolated the plasmids of strain v58 and have found that in addition to p, two further cryptic plasmids are also present. p has a size of 68 kb as determined by both electron microscopy and restriction endonuclease analysis. these other plasmids are 34 and 4.7 kb in size. restriction maps of p and the larger cryptic plasmid have been determined. it has be ... | 1987 | 2827202 |
| tetanus toxin binds with high affinity to neuroblastoma x glioma hybrid cells ng 108-15 and impairs their stimulated acetylcholine release. | differentiated neuroblastoma x glioma hybrid cells ng 108-15 express on their surface specific binding sites for tetanus toxin. 450 sites/cell with a kd of 2 x 10(-11) m were found under "physiological" conditions of ph and salt concentrations. a hill coefficient of 1.1 indicated noncooperative binding. specific binding of 125i-toxin to its sites could be prevented either by preincubation of the toxin with a neutralizing monoclonal antibody or by pretreatment of the cells with neuraminidase (vib ... | 1987 | 2826418 |
| comparison of solutions for extracting ck, ak, and ld from brain samples. | 1987 | 2826051 | |
| transient outward current prominent in canine ventricular epicardium but not endocardium. | previous studies have denied the presence of a transient outward current (ito) in ventricular myocardium of dog, sheep, and calf. using conventional microelectrode techniques, we provide evidence for a significant contribution of ito to epicardial, but not endocardial, activity of canine ventricular myocardium. the epicardial action potential when compared with that of endocardium shows a smaller phase 0 amplitude, a much more prominent phase 1, and a phase 2 amplitude that is greater than that ... | 1988 | 2826039 |
| transient outward current prominent in canine ventricular epicardium but not endocardium. | previous studies have denied the presence of a transient outward current (ito) in ventricular myocardium of dog, sheep, and calf. using conventional microelectrode techniques, we provide evidence for a significant contribution of ito to epicardial, but not endocardial, activity of canine ventricular myocardium. the epicardial action potential when compared with that of endocardium shows a smaller phase 0 amplitude, a much more prominent phase 1, and a phase 2 amplitude that is greater than that ... | 1988 | 2826039 |
| murine cytomegalovirus-pseudomonas synergistic infections: comparison of virulent and attenuated virus. | the synergistic interactions between pseudomonas aeruginosa and virulent or attenuated murine cytomegalovirus (mcmv) were compared in vivo. virulent mcmv challenge at a dose of 5 x 10(5) pfu/mouse intraperitoneally, followed by intranasal superinfection with 5 x 10(6) cfu/mouse of pseudomonas aeruginosa after 48 h resulted in greater than 80% mortality, apparently owing to a failure of pulmonary clearance mechanisms. single infections, or the use of attenuated mcmv in synergistic infections, did ... | 1987 | 2825950 |
| dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites. | selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ... | 1987 | 2825926 |
| dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites. | selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ... | 1987 | 2825926 |
| effects of caffeine and cyclic adenosine 3',5'-monophosphate on adenosine triphosphate-dependent calcium uptake by lysed brain synaptosomes. | the adenosine triphosphate-dependent calcium uptake by endoplasmic reticulum elements of lysed synaptosomes from rat brain cortex was studied. caffeine exhibited a biphasic effect on this calcium uptake activity: concentrations of 1, 2, 5, 10 or 30 mm caffeine stimulated calcium uptake by 62, 111, 73, 88 and 60% respectively, whereas calcium uptake was inhibited by 55% at a 60-mm concentration of caffeine. calcium release from endoplasmic reticulum elements of lysed brain synaptosomes was stimul ... | 1987 | 2825917 |
| chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex. | sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors. | 1987 | 2825912 |
| chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex. | sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors. | 1987 | 2825912 |
| opioid receptors inhibit the adenylate cyclase in guinea pig cochleas. | the effects of mu- and delta-preferring agonists on adenylate cyclase activity have been investigated in vitro in homogenates of guinea pig cochleas. morphine, leu-enkephalin, d-ala2, n-methyl-phe4, gly-ol5-enkephalin (dago) and d-ser2-leu-enkephalin-thr (dslet) each inhibited the synthesis of cyclic amp. this effect was reversed by naloxone which had a greater affinity in blocking the effect of the mu-preferring agonists (morphine, dago) than in blocking the effect of the delta-preferring agoni ... | 1987 | 2825909 |
| opioid receptors inhibit the adenylate cyclase in guinea pig cochleas. | the effects of mu- and delta-preferring agonists on adenylate cyclase activity have been investigated in vitro in homogenates of guinea pig cochleas. morphine, leu-enkephalin, d-ala2, n-methyl-phe4, gly-ol5-enkephalin (dago) and d-ser2-leu-enkephalin-thr (dslet) each inhibited the synthesis of cyclic amp. this effect was reversed by naloxone which had a greater affinity in blocking the effect of the mu-preferring agonists (morphine, dago) than in blocking the effect of the delta-preferring agoni ... | 1987 | 2825909 |
| the technique of dynamic adhesive strapping for congenital clubfoot. | the use of dynamic adhesive strapping to correct congenital clubfoot in the immediate neonatal period is an effective method of treatment. the technique is described in detail, the steps are illustrated, and precautions and limitations are given. this type of treatment should shorten the amount of time required to correct congenital clubfoot. if the holding phase is sufficiently prolonged, a reduction in the number and complexity of operative procedures needed for recurrent and recalcitrant case ... | 2010 | 2825884 |
| [bacteriologic studies of bicarbonate dialysate and suitable initial solutions]. | dialysates for the haemodialysis are produced unsterile and usually contain bacteria. own investigations of bicarbonate dialysate and adequate initial solutions comprised sterility tests, determinations of the germ count and germ tolerance experiments. only the "acid concentrate" was sterile. in the other solutions corynebacteria, acinetobacter and pseudomonas bacteria dominated as typical water germs. in the fresh reverse osmosis water and the bicarbonate dialysate as well as in the recently pr ... | 1987 | 2825447 |
| heart tumors specifically induced in young avian embryos by the v-myc oncogene. | to determine if expression of the v-myc oncogene had any effect during ontogeny, we injected avian myelocytomatosis virus strain mc29 into avian embryos at various stages of development. the injection of mc29 at embryonic day 2 (e2) or 3 (e3) caused, about 10 days later, rhabdomyosarcomas of the heart and, in some cases, skin muscle hypertrophy. when the injection was performed at e4 or e5, the number of heart tumors declined, whereas the number of skin muscle tumors increased significantly. the ... | 2009 | 2825173 |
| replication and packaging of choleraphage phi 149 dna. | the intercellular replication of the circularly permuted dna of choleraphage phi 149 involves a concatemeric dna structure with a size equivalent to six genome lengths. the synthesis of both monomeric and concatemeric dnas during replication of phi 149 occurred in the cytoplasm. the concatemers served as the substrate for the synthesis of mature phage dna, which was eventually packaged by a headful mechanism starting from a unique pac site in the concatemeric dna. packaging of dna into phage hea ... | 1987 | 2824829 |
| noradrenergic mechanisms in age-related cognitive decline. | the alpha-2 agonist, clonidine, improved spatial working memory performance in 13/13 aged rhesus monkeys with documented memory impairments. the clonidine response was blocked by alpha-2, but not alpha-1 antagonists, and appeared to result from actions at postsynaptic alpha-2 receptors in that area of cortex most critical for spatial working memory, the principal sulcal cortex. the data indicate that noradrenergic mechanisms play an important role in the functioning of the frontal association co ... | 1987 | 2824692 |
| functional homology of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, phosphoglycerate mutase, and 2,3-bisphosphoglycerate mutase. | the bisphosphatase domain of the rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase has been shown to exhibit a structural similarity to yeast phosphoglycerate mutase and human red blood cell 2,3-bisphosphoglycerate mutase including very similar active site sequences with a histidyl residue being involved in phospho group transfer. the liver bifunctional enzyme was found to catalyze the hydrolysis of glycerate 1,3-bisphosphate to glycerate 3-phosphate and inorganic phosphate. the km ... | 1987 | 2824507 |
| pulmonary complications of aids: henry ford hospital experience between january 1984 and july 1986. | 1987 | 2824409 | |
| synthesis of protein in intestinal cells exposed to cholera toxin. | the mechanism by which cyclic adenosine monophosphate (amp), formed by intestinal epithelial cells in response to cholera toxin, ultimately results in alterations in water and electrolyte transport is poorly understood. several studies have indicated that inhibitors of transcription or translation block much of the transport of ions and water in the intestine and edema formation in tissue elicited by cholera toxin. data presented in this study confirmed the inhibitory effects of cycloheximide on ... | 1987 | 2823275 |
| deferoxamine induced decreases of lipid peroxides in rheumatoid arthritis. | eleven patients with rheumatoid arthritis (ra) were treated with intraarticular deferoxamine or placebo to test the hypothesis that iron chelation decreased hydroxyl radical mediated lipid peroxidation in ra. intraarticular administration of deferoxamine 100 mg resulted in predominantly systemic effects with decreased serum ferritin and decreased serum levels of lipid peroxidation products. similar changes were not detected in synovial fluid at this dose. iron chelation with deferoxamine may pro ... | 2010 | 2822924 |
| dependence of an adenosine-activated potassium current on a gtp-binding protein in mammalian central neurons. | neurons in hippocampal and striatal cell cultures respond to adenosine with an inhibitory potassium current. this response disappears during whole-cell patch-clamp recording in which the cell is filled with minimal saline. we have found that this loss of sensitivity to adenosine can be prevented by including 100 microm gtp in the patch electrode filling solution. gdp is less effective than gtp in supporting the adenosine response, while gmp has little, if any, effect. treatments known to inhibit ... | 1987 | 2822865 |
| cloning and expression of the salmonella enterotoxin gene. | this report examines the genetic basis for salmonella typhimurium q1 enterotoxin production. a 918-base-pair xbai-hincii fragment of plasmid pjm17, composed of cholera toxin (ct) coding sequences (ctxab), was used as a gene probe. with this probe, the s. typhimurium enterotoxin was identified on a 6.3-kilobase ecori-psti fragment of chromosomal dna from plasmidless strain q1. we cloned this 6.3-kilobase fragment into escherichia coli rr1. the genetic map of the cloned salmonella enterotoxin (stx ... | 1987 | 2822664 |
| renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase c. | renal dipeptidase (dehydropeptidase-i, ec 3.4.13.11) was released from pig kidney membrane preparations by treatment with phosphatidylinositol-specific phospholipase c from staphylococcus aureus and bacillus thuringiensis and a phospholipase c preparation from bacillus cereus to a similar extent as alkaline phosphatase. endopeptidase-24.11 and aminopeptidase n were not released by this treatment. after treatment of the membrane fraction with the s. aureus phospholipase c the dipeptidase was conv ... | 1987 | 2822007 |
| renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase c. | renal dipeptidase (dehydropeptidase-i, ec 3.4.13.11) was released from pig kidney membrane preparations by treatment with phosphatidylinositol-specific phospholipase c from staphylococcus aureus and bacillus thuringiensis and a phospholipase c preparation from bacillus cereus to a similar extent as alkaline phosphatase. endopeptidase-24.11 and aminopeptidase n were not released by this treatment. after treatment of the membrane fraction with the s. aureus phospholipase c the dipeptidase was conv ... | 1987 | 2822007 |
| [anderson-fabry disease. current status. apropos of a case]. | after submitting a typical case of anderson-fabry disease in a 38 years old male, we discuss the eminent aspects of this metabolic disorder and, above all, the clinical, biochemical and ultrastructural problems of diagnosis in the heterozygotic relatives. | 2009 | 2821332 |
| [anderson-fabry disease. current status. apropos of a case]. | after submitting a typical case of anderson-fabry disease in a 38 years old male, we discuss the eminent aspects of this metabolic disorder and, above all, the clinical, biochemical and ultrastructural problems of diagnosis in the heterozygotic relatives. | 2009 | 2821332 |
| wilms tumors: relationship of nuclear deoxyribonucleic acid ploidy to patient survival. | nuclear deoxyribonucleic acid ploidy studies with paraffin-embedded archival tumor specimens were performed by flow cytometry on extracted nuclei from 56 wilms tumors. before the era of chemotherapy 9 patients had a 33 per cent survival rate at 5 years. no significant correlation between deoxyribonucleic acid ploidy pattern and survival was seen in this early group of patients. since 1960, 47 patients underwent radical nephrectomy and received chemotherapy. deoxyribonucleic acid histograms in th ... | 2010 | 2821294 |
| pure neural leprosy in a child: a case report. | pure neural leprosy developing in a 3 1/2 years old female child is reported. | 2009 | 2821133 |
| isotopic hybrids of nitrogenase. mössbauer study of mofe protein with selective 57fe enrichment of the p-cluster. | previous mössbauer and epr studies of the mofe protein (approximately 30 fe and 2 mo) of nitrogenase have revealed the presence of two unique clusters, namely, the p-clusters (presumably of the fe4s4 type) and the molybdenum- and iron-containing cofactors (or m-clusters). mössbauer components d (approximately 10-12 fe) and fe2+ (approximately 4 fe) represent subsites of the p-clusters while component s (approximately 2 fe) appeared to belong to a separate, unidentified cluster. in order to refin ... | 1987 | 2820958 |
| isotopic hybrids of nitrogenase. mössbauer study of mofe protein with selective 57fe enrichment of the p-cluster. | previous mössbauer and epr studies of the mofe protein (approximately 30 fe and 2 mo) of nitrogenase have revealed the presence of two unique clusters, namely, the p-clusters (presumably of the fe4s4 type) and the molybdenum- and iron-containing cofactors (or m-clusters). mössbauer components d (approximately 10-12 fe) and fe2+ (approximately 4 fe) represent subsites of the p-clusters while component s (approximately 2 fe) appeared to belong to a separate, unidentified cluster. in order to refin ... | 1987 | 2820958 |
| a new sv40-based vector developed for cdna expression in animal cells. | a useful vector, page103, has been developed for the expression of cdna in animal cells using the simian virus 40 (sv40) expression signals. cdna could be expressed easily by inserting it into the multiple cloning sites (hindiii, sali/acci, xbai, bamhi, smai/xmai, kpni/asp718, saci and ecori) of the vector, which are located between the sv40 early promoter and the sv40 early rna processing signals for splicing and polyadenylation. in addition to the above transcription unit, page103 contains the ... | 1987 | 2820955 |
| a new sv40-based vector developed for cdna expression in animal cells. | a useful vector, page103, has been developed for the expression of cdna in animal cells using the simian virus 40 (sv40) expression signals. cdna could be expressed easily by inserting it into the multiple cloning sites (hindiii, sali/acci, xbai, bamhi, smai/xmai, kpni/asp718, saci and ecori) of the vector, which are located between the sv40 early promoter and the sv40 early rna processing signals for splicing and polyadenylation. in addition to the above transcription unit, page103 contains the ... | 1987 | 2820955 |
| overproduction and purification of the tn3 transposase. | the tn3 transposase accumulated to about 4% of total cell protein in a minicell-producing escherichia coli strain harboring a transposase overproducer plasmid. this accumulation of the transposase seems to be due to four factors: derepression of transcription resulting from inactivation of the repressor gene (tnpr); efficient translation caused by a mutation within the shine-dalgarno (sd) sequence, the dosage effect of the increased plasmid copy number resulting from deletion of the rom gene of ... | 1987 | 2820953 |
| overproduction and purification of the tn3 transposase. | the tn3 transposase accumulated to about 4% of total cell protein in a minicell-producing escherichia coli strain harboring a transposase overproducer plasmid. this accumulation of the transposase seems to be due to four factors: derepression of transcription resulting from inactivation of the repressor gene (tnpr); efficient translation caused by a mutation within the shine-dalgarno (sd) sequence, the dosage effect of the increased plasmid copy number resulting from deletion of the rom gene of ... | 1987 | 2820953 |
| antibodies to polymerized human serum albumin in acute and chronic liver disease. | since antibodies to polyalbumin have been noted to occur in patients with hepatitis or cirrhosis, we investigated sera from 219 patients with a variety of acute and chronic liver diseases with and without hbsag using an elisa. the prevalence of circulating polyalbumin antibodies was shown to be significantly increased over that of healthy controls (6.7%) in patients with autoimmune chronic active hepatitis (67%), acute viral hepatitis (41%) and fulminant hepatic necrosis (38%), but such antibodi ... | 1987 | 2820857 |
| antibodies to polymerized human serum albumin in acute and chronic liver disease. | since antibodies to polyalbumin have been noted to occur in patients with hepatitis or cirrhosis, we investigated sera from 219 patients with a variety of acute and chronic liver diseases with and without hbsag using an elisa. the prevalence of circulating polyalbumin antibodies was shown to be significantly increased over that of healthy controls (6.7%) in patients with autoimmune chronic active hepatitis (67%), acute viral hepatitis (41%) and fulminant hepatic necrosis (38%), but such antibodi ... | 1987 | 2820857 |
| the molybdenum-pterin binding protein is encoded by a multigene family in clostridium pasteurianum. | there are three variants of the molybdenum-pterin binding protein (mop) encoded by three distinct genes in clostridium pasteurianum. nucleotide sequence analysis shows that the three mop genes have greater than 90% homology at the nucleotide level. upstream from the coding region of each mop gene are potential promoter consensus sequences. analysis of mop purified from cells grown under nitrogen-fixing conditions indicates all three genes are expressed. sequence analysis of the three mop genes a ... | 1987 | 2820842 |
| iga multiple myeloma coexistent with atypical adult t-cell leukemia. | we report a 65-yr-old male with adult t-cell leukemia (atl) who developed multiple myeloma (mm) concomitantly. skin lesions and peripheral leukocytosis were noted during the 5-yr observation period. there was abnormal lymphocytosis with indented or lobulated nuclei in the peripheral blood and in the bone marrow. the neoplastic cells reacted with monoclonal antibodies, okt3, okt4, okia1 and anti-tac. his serum was positive for the antibodies to atl-associated antigens. human t-cell leukemia virus ... | 1987 | 2820787 |
| iga multiple myeloma coexistent with atypical adult t-cell leukemia. | we report a 65-yr-old male with adult t-cell leukemia (atl) who developed multiple myeloma (mm) concomitantly. skin lesions and peripheral leukocytosis were noted during the 5-yr observation period. there was abnormal lymphocytosis with indented or lobulated nuclei in the peripheral blood and in the bone marrow. the neoplastic cells reacted with monoclonal antibodies, okt3, okt4, okia1 and anti-tac. his serum was positive for the antibodies to atl-associated antigens. human t-cell leukemia virus ... | 1987 | 2820787 |
| tn602: a naturally occurring relative of tn903 with direct repeats. | we report the characterization of tn602, a transposon encoding resistance to kanamycin and related aminoglycosides present on the r-plasmid pgd10. tn602 is highly homologous to the previously characterized tn903, present on the r-plasmid r6, in that it consists of a gene for aminoglycoside-phosphotransferase-3'-i (homologous to that of tn903) flanked by copies of an is-element homologous to is903. tn602 differs from tn903 in the following respects: the flanking is-elements (is602) are in direct ... | 1987 | 2819910 |
| regulation of nicotinic acetylcholine receptor phosphorylation in rat myotubes by forskolin and camp. | the nicotinic acetylcholine receptor (acchor) from rat myotubes prelabeled in culture with [32p]orthophosphate was isolated by acetylcholine affinity chromatography followed by immunoaffinity chromatography. under basal conditions, the nicotinic acchor was shown to be phosphorylated in situ on the beta and delta subunits. regulation of acchor phosphorylation by camp-dependent protein kinase was explored by the addition of forskolin or camp analogues to prelabeled cell cultures. forskolin, an act ... | 1987 | 2819883 |
| comparison of the structural characteristics of cell-cam 105 from hepatocytes with those of an altered form expressed by rat transplantable hepatocellular carcinomas. | the structural features of the adult rat hepatocyte (arh) forms of cell-cam 105, a mr 105,000 cell adhesion molecule, were compared using a variety of immunochemical and biochemical techniques with altered forms of more basic pi present on two transplantable hepatocellular carcinomas (thc 1682c and thc as-30d). immunoprecipitation analysis with polyclonal (anti-gp 105-2) and monoclonal (mab) antibodies specific for cell-cam 105 (mab 362.50) demonstrated that arh and thc cell-cam 105 were indisti ... | 1989 | 2819719 |
| review of evidence on the early detection and treatment of breast cancer. | 2010 | 2819675 | |
| production and partial characterization of elastase of vibrio cholerae inaba. | conditions are described for the production of extracellular elastase by vibrio cholerae inaba 2/57. the yield of the enzyme was maximum in shake cultures grown in alkaline peptone water at 37 degrees c and was stable in culture supernatants. the enzyme, partially purified by ammonium sulphate precipitation and sephadex g-100 gel filtration showed a molecular weight of 30,000 and an activity between ph 5.0-8.0 with an optimum of 8.0 in tris-maleate buffer. the elastinolytic activity was maximal ... | 1989 | 2818795 |
| [visualization of forensically important foreign bodies by direct roentgen magnification]. | the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ... | 1989 | 2818477 |
| [visualization of forensically important foreign bodies by direct roentgen magnification]. | the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ... | 1989 | 2818477 |
| a photographic study of enamel defects among south australian school children. | colour photographs were taken of the anterior teeth of 1757 consenting 8-9 year old primary school children in fluoridated and non-fluoridated areas of south australia. children with a lifelong exposure to fluoridated water were significantly more likely to have blemished permanent incisor teeth than the children who had never resided in a fluoridated area. children who resided in a non-fluoridated area but had taken fluoride supplements at some stage in their first five years had a prevalence o ... | 2009 | 2818303 |
| pancreatic cystadenoma. a clinicopathologic study. | a retrospective study of 19 patients with pancreatic cystadenoma included 15 patients with microcystic and 4 with mucinous cystadenomas. the typical clinical presentation was that of an elderly woman with an upper abdominal mass. an association with diabetes mellitus and extrapancreatic malignant disease was noted. total tumor resection provided the best chance of cure and removed the risk of compression of adjacent organs and, in mucinous cystadenomas, the risk of malignant transformation. | 2009 | 2818178 |
| false aneurysms after prosthetic reconstructions for aortoiliac obstructive disease. | several aspects of false aneurysm development after prosthetic resconstruction for aortoiliac obstructive disease were studied. for this purpose the long-term results (up to 20 years of follow-up) of 518 patients with implanted arterial prostheses in the aortoiliofemoral tract were retrospectively evaluated. completeness of follow-up data was 83.2% 15 years after operation. a total of 101 false aneurysms (21 aortic, 53 iliac, and 27 femoral) were detected in 69 patients and verified by operation ... | 2009 | 2818034 |
| the dermatopathologist and human immunodeficiency virus infection. | 1989 | 2817921 | |
| age-related morphologic differences in the components of the eustachian tube/middle ear system. | the current study utilized a three-dimensional computer graphics technique to analyze the size, shape, and positional associations of the eustachian tube (et) cartilage and lumen, the levator veli palatini (lvp) muscle, and the tensor veli palatini (tvp) muscle. older specimens 7 years and above (n = 13) were compared to young specimens less than 7 years (n = 4). our results suggest that changes in et function associated with age could result from size and shape differences in the et cartilage a ... | 2010 | 2817675 |
| the effect of urban trauma system hospital bypass on prehospital transport times and level 1 trauma patient survival. | we studied the influence of hospital bypass on prehospital times and level 1 trauma patient survival. during the nine-month study period, 251 level 1 trauma patients were transported to the cook county hospital trauma unit by chicago fire department (cfd) paramedics. the prehospital times and survival rates in the 203 (81%) patients who arrived with vital signs were analyzed. in this group, 64 (32%) had a hospital trauma score (ts) of 12 or less, 74 (39%) had at least one abbreviated injury scor ... | 2010 | 2817557 |
| failure of lower esophageal contractility to predict patient movement in children anesthetized with halothane and nitrous oxide. | the clinical usefulness of monitoring the frequency of spontaneous lower esophageal contractions (slec) to assess the depth of anesthesia was evaluated in 38 children. the hypothesis that slec can predict movement in response to skin incision during nitrous oxide and halothane anesthesia was tested. although movement in response to skin incision was correlated with an increase in heart rate, blood pressure, and an increased slec frequency, there was no correlation between the slec prior to skin ... | 2009 | 2817459 |