Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the value of laparoscopy in women with chronic pelvic pain and a "normal pelvis". | during the period january 1976--december 1982 laparoscopy was performed on 186 women complaining of pelvic pain of at least 6 months' duration. in all these cases, the routine pelvic examination and other medical and laboratory tests were negative. laparoscopy revealed pelvic pathology in only 8.2%: in the vast majority (91.8%) entirely normal pelvic organs were seen. evidence from the literature is compiling as to the psychogenic origin of most cases of chronic pelvic pain. recently, it has bee ... | 2010 | 2860038 |
| prolactin and its involvement in fluid regulation in the bovine. | plasma prolactin (prl) concentrations, osmolality, water consumption, feed intake, urine excretion, and fecal water output were determined in twelve steers of 3 breeds exposed to 5 feed and water regimes. breed differences were found in water intake and plasma prl concentrations when feed and water were ad lib., however, during any of the other 4 treatments, responses were similar between breeds. during dehydration and feed restriction, water intake, urine, fecal water, and plasma prolactin decr ... | 1985 | 2859961 |
| immunochemical recognition of oxytocin by antiserum to l-prolyl-leucyl-glycine amide. | antisera were raised in rabbits against pro-leu-gly-nh2 (plg)-bovine thyroglobulin conjugates. specificity of the antisera towards oxytocin was evaluated by a radioimmunoassay as well as by immunohistochemical procedures on hypothalamic tissue and hormone bound to sepharose beads. it was concluded that immunization with plg can raise antisera that are specific to oxytocin. | 1985 | 2859563 |
| the effects of midazolam and temazepam on sleep and performance when administered in the middle of the night. | a multicenter, double-blind, sleep laboratory and performance study was conducted to evaluate the hypnotic efficacy and residual effects of midazolam (15 mg) and temazepam (30 mg) compared to placebo when administered in the middle of the night. eighteen volunteers with objectively verified sleep maintenance insomnia received placebo for 3 nights during week 1 (adaptation and screening). during weeks 2, 3, and 4 they received 2 consecutive nights of midazolam, temazepam, and placebo (one treatme ... | 2008 | 2859305 |
| striatal synaptosomal dopamine synthesis: evidence against direct regulation by an autoreceptor mechanism. | regulation of the rate-limiting step in dopamine (da) synthesis was estimated in striatal synaptosomes by measuring the rate of hydroxylation of l-4-[3h]phenylalanine, a substrate of tyrosine hydroxylase (th). da inhibited hydroxylation with an ic50 of 0.2 microm. the concentration-response curve of da-induced inhibition was not affected by the presence of 1 microm chlorpromazine, a phenothiazine da antagonist. sulpiride and haloperidol, da antagonists of the benzamide and butyrophenone classes ... | 1985 | 2859213 |
| effects of a high-sucrose diet on the development of enzyme-altered foci in chemical hepatocarcinogenesis in rats. | dietary factors can modify metabolic events involved in the initiation, promotion, or progression of tumors. to determine whether a high-sucrose diet has any effect on the development of enzyme-altered foci during the promotion step of chemical hepatocarcinogenesis in rats, 1-day-old sprague-dawley rats were given a single i.p. dose of diethylnitrosamine; controls received an equivalent i.p. volume of 0.9% nacl solution. at 21 days of age, the rats were weaned, segregated by sex, separated in gr ... | 1985 | 2859110 |
| effects of a high-sucrose diet on the development of enzyme-altered foci in chemical hepatocarcinogenesis in rats. | dietary factors can modify metabolic events involved in the initiation, promotion, or progression of tumors. to determine whether a high-sucrose diet has any effect on the development of enzyme-altered foci during the promotion step of chemical hepatocarcinogenesis in rats, 1-day-old sprague-dawley rats were given a single i.p. dose of diethylnitrosamine; controls received an equivalent i.p. volume of 0.9% nacl solution. at 21 days of age, the rats were weaned, segregated by sex, separated in gr ... | 1985 | 2859110 |
| the antibacterial effect of selected neuroleptics on vibrio cholerae. | the present investigation has been undertaken to illustrate the anti-bacterial effect of chlorpromazine, clopenthixol and levomepromazine-maleate. it has been shown that clopenthixol has the strongest antibacterial effect. it is known that chlorpromazine also has an antisecretory activity. the fact that clopenthixol exists in cis(z)- and trans(e)-isomerical compounds, and that these compounds both have an antibacterial effect, but only the cis-compound has an antisecretory and neuroleptic effect ... | 1985 | 2858954 |
| preparation and properties of vibrio cholerae antifimbrial antibody. | the presence of fimbriae on the vibrio cholerae strains used was assessed by pellicle formation, haemagglutination activity and electron microscopy. fimbrial suspensions were prepared by shearing them off the organisms, then separating them from other components by absorbing them on to rabbit red blood cells. rabbits were then immunized with the fimbrial-red cell suspensions and the antibodies evoked were titrated by haemagglutination inhibition, agglutination, vibriocidal and immobilization tec ... | 1985 | 2858464 |
| polymicrobial aetiology of travellers' diarrhoea. | of 35 us peace corps volunteers in thailand, 20 (57%) had a total of 30 episodes of diarrhoea during their first 6 weeks in the country. enteric pathogens were associated with 90% of the episodes. a single pathogen was identified in 17 (57%) episodes, 2-4 pathogens were identified in 10 (33%) episodes, and there were 15 symptomless infections. enterotoxigenic escherichia coli (etec) was identified in 37% of these episodes, and various salmonella serotypes were isolated in 33%. infections with 9 ... | 1985 | 2857430 |
| skin cancer in renal transplant patients treated with cyclosporin. | 2009 | 2857227 | |
| complete nucleotide sequence coding for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a cdna clone. | cdna clones for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were isolated from rat liver expression libraries in lambda gt11 by antibody, oligonucleotide, and cdna screening. one 1860 bp long clone contained a full-length nucleotide sequence coding for the 470 amino acids of each of the two identical subunits of the bifunctional enzyme. this clone also contained untranslated sequences, one 173 bp long upstream from the atg start codon and one 271 bp long downstream from the tga stop cod ... | 1987 | 2856848 |
| a detailed study of cytomegalovirus infections in the first 160 heart and heart/lung transplant recipients at papworth hospital, cambridge, england. | 1987 | 2856284 | |
| has cyclosporine really relegated pretransplant blood transfusions to therapeutic obsolescence? | 1987 | 2856274 | |
| role of glycosyl phosphoinositides in insulin action. | 1988 | 2855505 | |
| outer membrane permeability in pseudomonas cepacia: diminished porin content in a beta-lactam-resistant mutant and in resistant cystic fibrosis isolates. | since beta-lactam resistance is a feature of pseudomonas cepacia isolates causing pulmonary infections in cystic fibrosis (cf), this study was undertaken to determine whether alterations in beta-lactam permeability mediate drug resistance in this species. a beta-lactam-susceptible non-cf isolate (strain 75-26), a resistant mutant derived from 75-26 by selection for cross-resistance to ciprofloxacin and ceftazidime, and two resistant cf isolates of p. cepacia were used. permeability constants wer ... | 1988 | 2855296 |
| [angiotensin converting enzyme (ace) activity in acromegaly]. | 2010 | 2854687 | |
| human endometrial proteins. | human endometrium synthesizes a number of proteins. two of the major proteins are the low molecular weight insulin-like growth factor-binding protein (igf-bp25) and endometrial protein pp14 (placental protein 14). both proteins have been cloned from human decidual cdna library and their complete amino acid sequences have been deduced. igf-bp25 is found in various tissues and body fluids including secretory and decidualized endometrium, amniotic fluid, liver, follicular fluid and luteinized granu ... | 1988 | 2854652 |
| in vitro activity of azithromycin (cp-62,993), a novel macrolide, against enteric pathogens. | azithromycin (cp-62,993 [9-deoxy-9a-methyl-9a-aza-9a-homoerythromycin]) is a novel macrolide antimicrobial. in this study the in vitro activity of cp-62,993 has been determined against selected enteropathogens, including clostridium difficile, and compared with that of erythromycin. mics were determined using an agar incorporation technique in mueller-hinton medium, containing saponin-lysed horse blood at a final concentration of 10% v/v, with an inoculum of 10(4) cfu. cp-62,993 was considerably ... | 1988 | 2854515 |
| establishment and characterization of an epstein-barr virus negative b-cell lymphoma cell line and successful heterotransplantation. | an ebv-negative b-cell lymphoma cell line designated as hbl-1 was established from the pleural effusion of a patient with malignant lymphoma, diffuse, large cell. surface marker studies revealed that hbl-1 cell possessed the same phenotypes of a mature b-cell as the original lymphoma cell from pleural effusion. hbl-1 closely resembled morphologically to the original lymphoma cells observed in the lymph node biopsy and pleural effusion. chromosome analysis revealed that hbl-1 cells showed chromos ... | 1988 | 2854303 |
| phenyl-t-butylnitrone, a free radical spin-trapping agent, inhibits acetylcholine-induced relaxation and cyclic gmp accumulation in rat thoracic aorta. | 1988 | 2854109 | |
| a novel peptide vasoconstrictor, endothelin, is produced by vascular endothelium and modulates smooth muscle ca2+ channels. | since the discovery of endothelium-dependent vasodilation, vascular endothelium has been recognized as an important functional unit contributing to the regulation of vascular tonus. recent purification, structure determination and molecular cloning of a novel peptidergic vasoconstrictor, endothelin, from the culture supernatant of porcine aortic endothelial cells has further substantiated this concept. starting from a large quantity of serum-fee endothelial cell-conditioned medium, endothelin wa ... | 1988 | 2853725 |
| phorbol ester and dibutyryl cyclic amp reduce content and efflux of taurine in primary cerebellar astrocytes in culture. | in 16-18 days in vitro (div) primary astrocyte cultures prepared from 7- to 9-day-old rats, 48 h exposure to 12,13-phorbol dibutyrate (pdbu) (1 microm) or dibutyryl camp (dbcamp) (1 mm) reduced cellular taurine content, and both basal and 50 mm k+-evoked taurine efflux, but did not alter cellular glutamate or total protein content. decreases in cellular taurine content first became apparent between 1 and 6 h and were maximal after 24 h. treatment also rapidly altered astrocyte morphology to a mo ... | 1988 | 2852322 |
| [changes in the cyclic nucleotide system of t-lymphocytes in experimental tuberculosis in animals]. | 1988 | 2851783 | |
| glomus tumor of the trachea. immunohistochemical and electron microscopic studies. | glomus tumor of the trachea which developed in a 51-year-old man was reported. he experienced frequent episodes of respiratory infections and hemoptosis for 9 months. bronchofiberscopic study demonstrated a polypoid tumor in the upper trachea. local resection of the tumor was performed and it arised from the membranous portion of the third and fourth tracheal rings. histologically, the tumor was composed of medium-sized rounded cells, resembling carcinoid tumor. desmin and myosin immunoreactivit ... | 1988 | 2851778 |
| transfer of pre-embryos donated and fertilized in vivo to recipients given a steroid replacement therapy. | this study describes eleven procedures of non-surgical recovery of preimplantation conceptuses from the uteri of eight fertile donor women and the transfer of the recovered pre-embryos into the uteri of infertile recipients with primary ovarian failure, or with hypothalamic--pituitary failure. conjugated oestrogens and progesterone were administered to recipients as replacement steroids during the first 20 weeks of pregnancy, in order to produce a secretory endometrium allowing nidation and deve ... | 2010 | 2851614 |
| epidermal growth factor-stimulated dna synthesis requires an influx of extracellular calcium. | the dependency of normal cell proliferation on adequate extracellular ca2+ levels was further investigated by determining the role of ca2+ influx in epidermal growth factor (egf)-induced rat liver epithelial (t51b) cell dna synthesis. fura-2-loaded t51b cells responded with an increase in [ca2+]i to egf (5-50 ng/ml) that was blocked by low (25 microm) extracellular ca2+ or by pretreatment with 50 microm la3+ to inhibit plasma membrane ca2+ flux. confluent t51b cells treated for 24 h with egf (0. ... | 1988 | 2851595 |
| calcium-permeable channels in vascular smooth muscle: voltage-activated, receptor-operated, and leak channels. | 1987 | 2850612 | |
| [hemostatic tests for the diagnosis of thrombosis. endothelial cell function]. | 2010 | 2850372 | |
| protein kinase c activities are increased in rat thyroid epithelial cells expressing v-ras genes. | both cytoplasmic and membrane-bound protein kinase c activities are increased in: harvey-sarcoma virus, infected thyroid epithelial cells. the cytoplasmic kinase c increase is found in the chromatographic fraction eluted at lower salt concentration (100 mm nacl-s100), while the more acidic protein fraction eluted at higher salt concentration (35 mm nacl-s350) is virtually absent. although the cytoplasmic s100 fraction from the control and ras-virus infected cells display a comparable pbt2 bindin ... | 1988 | 2849929 |
| effect of na+/h+ exchange inhibitors on agonist-induced contraction of rat aorta. | a number of vasoconstrictor agonists activate the na+/h+ antiport system in vascular smooth muscle, leading to alkalinization of the cytosol and influx of na+. it is believed that agonist-induced na+/h+ exchange may play an important role in contraction. we have evaluated this hypothesis by determining the effect of inhibition of na+/h+ exchange on phenylephrine (pe)-induced contraction of rat aorta. preincubation of rat aorta with the na+/h+ exchange inhibitor amiloride (0.5 mm) inhibited subse ... | 1988 | 2849662 |
| suppression of sv40-promoted gene expression by differentiation of preadipose cells. | when a plasmid bearing the chloramphenicol acetyltransferase (cat) gene under the control of an sv40 early promoter is introduced into preadipose or adipose cells of line 3t3-f442a, the promoter directs high levels of transient expression of cat. however, when the plasmid is introduced into preadipose cells and the cells are then allowed to differentiate into adipose cells, the expression of the cat gene is suppressed. in this process, the plasmid is not changed detectably in amount, topology, o ... | 1988 | 2849579 |
| avian nephroblastomas induced by a retrovirus (mav-2) lacking oncogene. ii. search for common sites of proviral integration in tumour dna. | to demonstrate the existence of a common site of integration in independent tumour clones, restriction mapping of the vicinity of integrated mav-2 proviruses in nephroblastoma dna was performed, using southern hybridization with an mav-2 specific probe u3(pat). the results have shown that (1) nephroblastomas are of semiclonal origin. (2) nephroblastoma cells contain an average of 5 clonally located integrated proviruses per diploid genome; they do not contain any detectable amount of non-integra ... | 1988 | 2849568 |
| [immunologic diagnosis of cytomegalovirus infections in patients after kidney transplantation]. | 1988 | 2849513 | |
| kininase ii from human seminal plasma. isolation and properties. | a rapid and highly efficient procedure for purification of kininase ii from human seminal plasma is described. after ultra centrifugation, the enzyme was purified by gel filtration on sepharose 6b cl and ion exchange chromatography followed by affinity chromatography of edta-inhibited enzyme on bradykinin-sepharose. the enzyme was specifically inhibited by captopril and bpp9a but not by phosphoramidon. page in the presence of sodium dodecyl sulfate under reducing conditions resulted in two major ... | 1988 | 2849459 |
| a new class of free radical scavengers reducing adriamycin mitochondrial toxicity. | beef heart mitochondria were incubated with adm and nadh. an adriamycin semiquinone radical was detected using esr spectroscopy. the semiquinone radical production rate is decreased upon addition of a scavenger (ad 20) in the reaction medium. nmri mice were treated with ad 20 (70 mg/kg, i.p.) 15 min prior adm injection (20 mg/kg, i.p.) or with adm alone. heart mitochondria were isolated 48 hr later. the enzymatic activities of complex i-iii and complex iv of the mitochondrial respiratory chain w ... | 1988 | 2849451 |
| muscarinic receptor coupling to inositol phospholipid metabolism in guinea-pig cerebral cortex, parotid gland and ileal smooth muscle. | inositol phospholipid hydrolysis induced by agonist-stimulation of muscarinic receptors has been examined in slices of guinea-pig cerebral cortex, parotid gland and ileal smooth muscle. an assay measuring 3h-inositol phosphate formation from prelabelled lipids in the presence of licl, allowed marked stimulation by agonists to be followed. the pd2-value of carbachol differed markedly, between tissues being more than 10-fold lower in cerebral cortex than in parotid gland. the partial agonist oxotr ... | 1988 | 2849446 |
| properties and developmental regulation of the protein phosphatases in dictyostelium discoideum. | the properties and developmental regulation of the protein phosphatases of dictyostelium discoideum were examined. when crude extracts from vegetative cells were separated on a mono q column (fplc) three protein phosphatase peaks, designated p1, p2 and p3 were found. when aggregation and culmination cells were examined only one protein phosphatase peak was observed. this corresponded to phosphatase p1 of vegetative cells. all three of the vegetative cell phosphatase were inhibited by heparin and ... | 1988 | 2849442 |
| plasma from uninephrectomized rats stimulates production of inositol trisphosphates and inositol tetrakisphosphate in renal cortical slices. | metabolism of inositol phosphates in renal cortical slices was investigated in vitro after addition of plasma from uninephrectomized or sham-operated rats. plasma from uninephrectomized rats stimulated production of insp3 (inositol trisphosphate) when obtained within the first 3 h after uninephrectomy. with different amounts of added plasma a graded response of insp3 production was obtained. this effect could be prevented by 0.1 microm-tpa (12-o-tetradecanoylphorbol 13-acetate). when analysis of ... | 1988 | 2849423 |
| salience and the effects of cs preexposure on aversion conditioning. | rats (n = 84) received preexposure to distilled water or to one of two differently salient flavors, 5.0% casein or 10.0% sucrose, casein being the more salient. each preexposure group then received aversion conditioning to a 5.0% casein or a 10.0% sucrose cs. aversion effects were reliably more enduring to casein than to sucrose. relative to water-preexposed groups, preexposure to casein attenuated aversion effects to the casein cs reliably less than preexposure to sucrose attenuated aversion ef ... | 1988 | 2849411 |
| retention enhancement by pre-test beta-endorphin and oxotremorine and its reversal by scopolamine. | the pre-test administration of beta-endorphin (0.05 microgram/mouse), oxotremorine sesquifumarate (5.0 micrograms/mouse), or beta-endorphin plus oxotremorine enhanced retention test performance of a conditioned emotional response in mice. the effects were blocked by scopolamine. scopolamine had no effect of its own. the data suggest that the retention enhancement caused by beta-endorphin may involve the secondary activation of cholinergic mechanisms. | 1988 | 2849408 |
| post-epizootic surveys of waterfowl for duck plague (duck virus enteritis). | surviving birds from nine duck plague outbreaks in urban and confined waterfowl were sampled for duck plague (dp) virus and dp antibody during 1979-86. duck plague virus was found in combined oral and cloacal swabs of birds from three outbreaks, and dp-neutralizing antibody was demonstrated in some birds from all nine outbreaks. greater prevalence of dp antibody and higher titers were found in survivors from confined populations than from free-flying urban populations. free-flying waterfowl from ... | 1988 | 2849402 |
| 99mtc dimercaptosuccinic acid (dmsa) scan as first investigation of urinary tract infection. | a total of 115 children under 5 years who presented with a first symptomatic urinary tract infection and who had a 99mtc dimercaptosuccinic acid (dmsa) scan were studied to assess its value and compare the findings with those of other imaging techniques. renal cortical defects were detected in 65 kidneys by dmsa scan, intravenous urogram, and ultrasound scan combined; 62 (95%) being seen on dmsa scan. the finding of reflux on micturating cystourethrography showed a highly significant correlation ... | 2010 | 2849382 |
| fatal asthma in a subject sensitized to toluene diisocyanate. | we report the case of a 43-yr-old car painter who died within 1 h of exposure to a polyurethane paint in the workplace. a diagnosis of asthma induced by toluene diisocyanate (tdi) had been established 6 yr before, when he underwent inhalation challenges with carbachol and with tdi. the subject had airway hyperresponsiveness to carbachol (pd20fev1 carbachol = 0.32 mg; normal value greater than 1.0 mg) and developed an early and long-lasting asthmatic reaction after exposure to tdi in the laborato ... | 2010 | 2849334 |
| [the relationship between transport site of hpd and photodamage region of human gastric cancer cells in the cell cycle]. | 1988 | 2849266 | |
| expression of env sequences of the bovine leukemia virus (blv) in the yeast saccharomyces cerevisiae. | dna sequences of the envelope (env) gene of the bovine leukemia virus (blv) were expressed in the yeast saccharomyces cerevisiae. two yeast promoters, the repressible pho5 promoter and the constitutive pgk promoter, were used to construct four expression plasmids comprising either a sequence of the surface antigen gp51 or a (gp51 + gp30) sequence. the expressed heterologous gene products were characterized by western blot analysis and competitive radioimmunoassay. by means of northern blot analy ... | 1988 | 2849260 |
| ph-dependent accumulation of the vesicular stomatitis virus glycoprotein at the ends of intact virions. | the electron microscope was employed to examine the effect of ph on the distribution of the vesicular stomatitis virus glycoprotein (vsv g-protein) on the surface of intact virions. both rotary shadowed and negatively stained preparations demonstrated that whereas at neutral ph the glycoprotein was evenly distributed along the virus membrane, at ph 5.5 it was concentrated at the ends giving the overall virion a distinct bilobed or bipolar morphology. negatively stained specimens showed that the ... | 1988 | 2849241 |
| [electromyographic findings in patients with the vertebral syndrome associated with polyneuropathy]. | 2007 | 2849181 | |
| [99mtc-nanocolloid: clinical results of its use as a new inflammation marker]. | fifty scintigrams were performed on 45 patients using 99mtc-nanocolloid in order to test its affinity for inflammatory lesions; in these patients there was clinical and/or radiological suspicion of an inflammatory process. there was good correlation between the degree of uptake and the activity of the inflammatory process. specificity is limited because of uptake in certain tumours (eg. sarcomas of the extremities), but difficulties in differential diagnosis are not expected to be serious. judgi ... | 2008 | 2849165 |
| a major outer membrane protein in vibrio cholerae is maltose-inducible. | 1987 | 2849017 | |
| effect of cepharanthin on superoxide anion (o2-) production by macrophages. | 2008 | 2848880 | |
| transcriptional regulation of the gene encoding the major surfactant protein (sp-a) in rabbit fetal lung. | the expression of the major protein of rabbit pulmonary surfactant (sp-a), a glycoprotein of mr congruent to 29,000-36,000, is regulated during development and by hormones. in the present study, utilizing a cdna insert complementary to mrna coding for sp-a and nuclear transcription elongation assays, we have investigated the developmental and hormonal regulation of transcription of the sp-a gene in rabbit fetal lung tissue. the relative rates of transcription of sp-a mrna increased as a function ... | 1988 | 2848814 |
| role of cytochrome c heme lyase in the import of cytochrome c into mitochondria. | the import of cytochrome c into neurospora crassa mitochondria was examined at distinct stages in vitro. the precursor protein, apocytochrome c, binds to mitochondria with high affinity and specificity but is not transported completely across the outer membrane in the absence of conversion to holocytochrome c. the bound apocytochrome c is accessible to externally added proteases but at the same time penetrates far enough through the outer membrane to interact with cytochrome c heme lyase. format ... | 2009 | 2848813 |
| enzymatic introduction of a fluorescent sialic acid into oligosaccharide chains of glycoproteins. | aiming at the introduction of a fluorescent sialic acid into glycoconjugates, 5-acetamido-9-(3-fluoresceinylthio-ureido)-3,5,9-trideoxy-2-non ulosonic acid (9-fluoresceinyl-neuac) was synthesized which has an intact carbon chain. a) despite the space-filling substituent at c-9, the fluorescent neuac analogue was activated to the corresponding cmp-glycoside by cmp sialic acid synthase from bovine brain. whereas the km value of the synthase was little affected by the modification (km = 2.1 mm, for ... | 1988 | 2848703 |
| the role of diffusible molecules in regulating the cellular differentiation of dictyostelium discoideum. | a central problem in developmental biology is to understand how morphogenetic fields are created and how they act to direct regionalized cellular differentiation. this goal is being pursued in organisms as diverse as moulds, worms, flies, frogs and mice. each organism has evolved its own solution to the challenge of multicellularity but there appear to be common underlying principles and, once pattern formation is fully understood in any system, some general truths seem certain to be revealed. a ... | 1988 | 2848678 |
| sensitive elisa for the gp120 and gp160 surface glycoproteins of hiv-1. | we have used a panel of polyclonal and monoclonal antibodies against gp120 and gp160, the envelope glycoproteins of human immunodeficiency virus type 1, to create rapid, simple, and sensitive twin-site sandwich elisa specific for gp120 and gp160 or for gp160 alone. these assays can detect 500 cos cells in a population transiently transfected with a construct encoding gp120 and gp160, or 50 pg of recombinant gp160. we estimate that the mean amount of gp120 + gp160 in the transfected population is ... | 1988 | 2848557 |
| conjugation in saccharomyces cerevisiae. | 1988 | 2848554 | |
| transcriptional and translational events during reovirus infection. | this short review focuses on the mechanisms involved in transcription and translation in mouse l cells infected with reoviruses. the viral genomic rna (double-stranded), retained in the inner capsid following removal of the outer capsid of the infecting virion, is transcribed by a viral polymerase. the synthesized viral mrna is blocked at the 5' end by a cap structure similar to the cap structure of cellular mrna but synthesized by the viral enzymes of the inner capsid. this viral mrna is also u ... | 1988 | 2848543 |
| isolation and characterization of a specific receptor for human albumin on a group l streptococcus. | certain group l streptococci demonstrate surface receptors for human albumin. binding of 125i-albumin to group l streptococci could be inhibited by unlabelled albumin preparations from humans, dogs, mice and bovines, but not by albumin from rabbits. the albumin-binding proteins (abp) could be solubilized from the streptococcal surface by hot acid treatment of the bacteria and isolated by affinity chromatography on human-albumin sepharose. abp and specific antisera produced against abp inhibited ... | 1988 | 2848386 |
| inhibition of porphyrin biosynthesis by exogenous 5-aminolevulinic acid in an aerobic photosynthetic bacterium, erythrobacter sp. och 114. | exogenously administered 5-aminolevulinic acid (ala) inhibited the formation of bacteriochlorophyll a (bchl a) in a dose-dependent manner in the aerobic photosynthetic bacterium, erythrobacter sp. strain och 114, under dark growth conditions. the ala concentration required for half-inhibition after 24-h growth was estimated to be about 3.0 mm. porphyrin and bchl precursors were not found in either the cells or the growth medium. the same inhibition was also observed with cytochrome c formation. ... | 1988 | 2847654 |
| blunted cgmp response to anf in vascular smooth muscle cells of shr. | abnormalities in the coupling of atrial natriuretic factor (anf) receptors with the guanosine 5'-cyclic monophosphate (cgmp) system in vascular smooth muscle cells (vsmcs) may play a role in the pathophysiology of hypertension in the spontaneously hypertensive rat (shr). this concept was examined in cultured, aortic vsmcs (passages 6-10) from shr, wistar-kyoto (wky), and american wistar (wis) rats. quiescent vsmcs of the shr (serum deprived for 24 h) had higher anf receptor density (bmax) and lo ... | 1988 | 2847533 |
| [galactography in the early detection of breast cancer]. | pathologic discharge from the breast occurred in 308 out of 5000 (6.1%) apparently healthy women. ductography was carried out in 237 such cases. the procedure revealed ductal ectasia in 96 patients (40.5%), ductal papillomatosis--38 (16.0%), intraductal papilloma--15 (6.8%) and cysts--in 16 cases. cancer was suspected in 22 (9.3%) patients and in 14 (63.6%) of them tumor was diagnosed following excisional biopsy. on the whole, cancer was detected in 5.9% of the examined cases. in 13 out of 14 (9 ... | 2009 | 2847429 |
| influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines. | sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ... | 1988 | 2847397 |
| [effect of bilirubin and serum albumin on the na,k-atpase activity in the synaptosomal membrane]. | the absorption spectrum of visible light, characteristic of the free bilirubin being in the aqueous medium, with a single maximum at 440 nm and with the shoulder in the region of 410-420 nm is transformed into the spectrum with two maxima in the region of 460 and 500 nm, respectively, when the pigment is bound in vitro by the synaptosomal membrane. there are two types of sites for bilirubin binding in the membrane particles, differing in the values of constants of association (ka = 0.6 . 10(5) a ... | 1988 | 2847380 |
| primary liver tumors in children: comparison of ct and mr imaging. | a prospective study was performed at two institutions to determine the role of magnetic resonance (mr) imaging in the evaluation of primary pediatric liver tumors. the study compared the usefulness of mr imaging and computed tomography (ct) in providing a correct diagnosis, assessing resectability, and determining tumor recurrence in 23 children with liver tumors. the information obtained with both modalities was correlated with surgical data. the results indicate that the accuracy of mr imaging ... | 2009 | 2847233 |
| primary liver tumors in children: comparison of ct and mr imaging. | a prospective study was performed at two institutions to determine the role of magnetic resonance (mr) imaging in the evaluation of primary pediatric liver tumors. the study compared the usefulness of mr imaging and computed tomography (ct) in providing a correct diagnosis, assessing resectability, and determining tumor recurrence in 23 children with liver tumors. the information obtained with both modalities was correlated with surgical data. the results indicate that the accuracy of mr imaging ... | 2009 | 2847233 |
| txb2: cardiostimulant effect that involves beta-adrenoceptor and na+ + k+-atpase activity. | the biological properties of thromboxane b2 (txb2) on isolated rat heart were studied. its actions were compared with u-46619 a thromboxane a2 mimetic compound and with isoproterenol. txb2 induced a concentration-dependent increase in contractility, that was non-competitively antagonized by propranolol. in addition txb2 inhibited na+ + k+-atpase activity at the same concentrations that influenced the mechanical activity. inhibition of beta-adrenoceptors efficiently blocked the inhibitory action ... | 1988 | 2847200 |
| cellular mechanism of ischemic acute renal failure: role of ca2+ and calcium entry blockers. | this presentation briefly reviews the cellular mechanism of ischemic acute renal failure (arf) with particular emphasis on the role of ca2+ and calcium entry blockers (ceb). vascular consequences of an ischemic renal insult including vasoconstriction, diminished glomerular permeability, loss of autoregulation, and hypersensitivity to renal nerve stimulation may relate to increased cellular ca2+ concentration in the renal afferent arteriole and glomerular mesangial cells. evidence is also present ... | 2010 | 2846945 |
| infection control measures for human immunodeficiency virus (hiv): universal precautions. | 1988 | 2846803 | |
| endobronchial hamartoma associated with bronchioloalveolar cell carcinoma. | computed tomographic findings in a 60-year-old man with lobar bronchioloalveolar cell carcinoma distal to endobronchial hamartoma are described. | 1988 | 2846241 |
| heterogeneity of glycine receptors and their messenger rnas in rat brain and spinal cord. | messenger rnas isolated from adult or newborn rat spinal cord were fractionated in a sucrose gradient. the fractions were injected into xenopus oocytes to determine their potencies for expression of glycine receptors (glyrs), which were then examined electrophysiologically. the sedimentation profiles disclosed two classes of glyr mrnas, one heavy and the other light. the adult spinal cord was rich in heavy glyr mrna, whereas the light glyr mrna was more abundant in neonatal spinal cord and in ad ... | 1988 | 2845580 |
| [the diagnosis of infectious mononucleosis in childhood]. | 1988 | 2845334 | |
| leukotriene d4-induced homologous desensitization in basal and differentiated u-937 cells: characterization with the partial agonist leukotriene e4 and assessment of receptor reserve. | u-937 cells express receptors for leukotriene d4, and ltd4 stimulates receptor-mediated ca++ mobilization with an ec50 of 5 nm. in these cells, lte4 produces ca++ mobilization with an ec50 of 500 nm and a maximum response that is 15 to 20% of the ltd4 response. lte4 was shown to be a partial agonist, with a kp of 400 nm and a linear relationship between effect and receptor occupancy, and was used as a tool to study ltd4-induced desensitization. pretreatment of u-937 cells with ltd4 resulted in a ... | 2010 | 2845060 |
| production of antibodies against murine class ii antigens using unconjugated synthetic peptides of the beta chain region 63-78. | synthetic peptides corresponding to residues 63-78 of the first domain of the beta chain of murine i-a/i-e class ii antigens were used in the unconjugated rather than the traditional protein-conjugated form to immunize (129j x b6)f1 mice. the sequences made represented the four haplotypes; ak beta, ad beta, abm-12 beta and ed beta. these sequences were selected on the basis of computer algorithms used to predict surface accessibility and main-chain flexibility profiles, and by reported hypervari ... | 2010 | 2844910 |
| t4 cell activation by immobilized phytohemagglutinin: differential capacity to induce il-2 responsiveness and il-2 production. | soluble mitogens, such as pha induce accessory cell (ac)-dependent t cell proliferation. one function of the ac is to create a stimulatory matrix. therefore, experiments were carried out to determine whether pha immobilized onto microtiter plates could stimulate t cells in the absence of ac. peripheral blood t4 cells were cultured under limiting dilution conditions with either soluble or immobilized pha with or without ril-1 beta, ril-2, r-tnf-alpha, an anti-cd28 mab (9.3), or irradiated ebv-tra ... | 1988 | 2844904 |
| automated synthesis system with computer control for the production of [1-11c]fatty acids. | to accommodate the increasing need for [1-11c]fatty acids for use in routine medical studies and the large amounts of radioactivity required, a more advanced automated synthesis system for the preparation of [1-11c]fatty acids has been constructed. the synthesis of [1-11c]fatty acids has been completely automated using a new separation unit (isobaric sepaltor) for the extraction procedure, which had caused some difficulties with automation. the yield of [1-11c]palmitic acid was 20-30 mci, and th ... | 2010 | 2844700 |
| extraretroperitoneal abdominal malignant fibrous histiocytoma. | seven cases of extraretroperitoneal abdominal malignant fibrous histiocytoma (mfh) are reported. the anatomic distribution was as follows: liver (2 cases), spleen with gastric and pancreatic involvement (1 case), mesentery (1 case), right mesocolon (1 case), left mesocolon (1 case), and urachus (1 case). these bulky lesions were often extensively necrotic; calcifications were seen in 2 cases. all 7 cases were investigated by ultrasonography (us) and computed tomography (ct). barium studies were ... | 2010 | 2844619 |
| detection of leukotriene b4 in cardiac tissue and its role in infarct extension through leucocyte migration. | the main left coronary artery of rats was ligated near its origin under light ether anaesthesia and the infarction observed for 48 h. the ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride. the unstained area (true ischaemic area) decreased with time to 27.1% of the left ventricle at 48 h, whereas the intensely stained area between the normal and the true ischaemic areas increased with time, sugges ... | 1988 | 2844405 |
| [relation between the characteristic activity of the beta-adrenergic agonist isoproterenol and the percolation properties of cell membrane]. | variations in the intrinsic activity of 1-isoproterenol and in the percentage of high affinity beta-receptor complexes have been studied under a changes in the macrostructure of reticulocyte plasma membranes. the fluid lipid fraction have been reduced in the membranes for this by phospholipase a2/bsa treatment. it was accompanied by a progressive decrease in the fraction of beta-receptors that are able to form the high affinity complexes with 1-isoproterenol (receptor--regulatory n-protein compl ... | 1988 | 2844324 |
| effect of ethanol on neuromuscular function in the rat diaphragm preparation. | the effect of ethanol on neuromuscular transmission in the isolated phrenic nerve-diaphragm preparation of the rat was studied by analysing its effect on directly- and indirectly-elicited twitch tension, tetanic and post-tetanic twitch tensions, and on the phenomenon of post-tetanic twitch potentiation (ptp). the results show that ethanol (1.0 x 10(-4)-1.0 m) depresses neuromuscular transmission, by reducing the amplitudes of both directly and indirectly elicited twitch and tetanic contractions. ... | 1988 | 2844197 |
| percutaneous sonographic needle aspiration biopsy of endoscopically negative gastric carcinoma. | we report here the use of ultrasound-guided percutaneous fine needle aspiration gastric biopsies in three patients with endoscopically negative biopsies. two men, ages 60 and 64, and one woman, age 64, with signs and symptoms of weight loss, abdominal pain, and early satiety, had barium contrast studies suggestive of thickened gastric walls of the antrum and cardia, yet multiple endoscopic biopsies were negative for malignancy. using real time sector b-scan ultrasonography, percutaneous fine nee ... | 2007 | 2844079 |
| rna polymerase ii allows unwinding and rewinding of the dna and thus maintains a constant length of the transcription bubble. | ternary complexes of rna-dna-rna polymerase ii, originating from the in vivo transcriptionally active sv40 minichromosomes, can be detected and analyzed by a method previously developed (choder, m., bratosin, s., and aloni, y. (1984) embo j. 3, 2929-2936). using this method, we compared the electrophoretic mobilities of sv40 ternary complexes with those of sv40 rna-dna complexes obtained after the removal of the polymerases. independent of the in vitro elongation of the nascent rna, topoisomers ... | 2009 | 2843504 |
| thiol peroxyl radical formation from the reaction of cysteine thiyl radical with molecular oxygen: an esr investigation. | using electron spin resonance (esr) spectroscopy, we have identified the cysteine thiol peroxyl radical (cyssoo.) at low temperatures in two aqueous glasses. this radical shows a typical peroxyl radical esr spectrum, but unlike carbon-based peroxyl radicals has a violet color (lambda max = 540 nm) and forms a new radical showing a singlet esr spectrum when photobleached with visible light. the cysteine peroxyl radical reacts to form the cysteine sulfinyl radical (cysso.) in the glass which allow ... | 2010 | 2843184 |
| cyclic nucleotide phosphodiesterase activity: histochemical and cytochemical methods. | 2009 | 2842613 | |
| cyclic nucleotide phosphodiesterase activity: histochemical and cytochemical methods. | 2009 | 2842613 | |
| primary mediastinal germ cell tumors. histologic patterns of treatment failures at autopsy. | twenty-five patients presented with primary mediastinal germ cell tumors at roswell park memorial institute between 1959 and 1984. all patients were treated by surgery and chemotherapy with or without radiotherapy. four patients are still alive, and 21 patients died of mediastinal germ cell tumor and its sequelae. two patients were found to have testicular scars and were dropped from the study. nongerm cell malignant transformation of a teratoma occurred in five of the remaining 17 patients (29% ... | 2010 | 2842031 |
| egg shell quality and cholecalciferol metabolism in aged laying hens. | calcium-binding protein d28k (calbindin) synthesis, vitamin d metabolism and shell quality were investigated in young and aged laying hens fed diets containing either cholecalciferol (cc) or its 1-hydroxylated derivatives. duodenal calbindin concentration was similar in the young and in the aged laying hens. exogenous 1-hydroxylated cc derivatives increased duodenal calbindin concentration, regardless of age. shell weight and shell density (mg/cm2) were significantly lower (p less than 0.01) in ... | 1988 | 2841436 |
| molecular defects in cytochrome oxidase in mitochondrial diseases. | defects of cytochrome c oxidase (cox) show remarkable clinical, biochemical, and genetic heterogeneity. clinically, there are two main groups of disorders, one dominated by muscle involvement, the other by brain dysfunction. biochemically, the enzyme defect may be confined to one or a few tissues (reflecting the existence of tissue-specific isozymes) or affect all tissues. immunologically reactive enzyme protein is decreased in some forms of cox deficiency but not in others. because cox is encod ... | 1988 | 2841309 |
| molecular defects in cytochrome oxidase in mitochondrial diseases. | defects of cytochrome c oxidase (cox) show remarkable clinical, biochemical, and genetic heterogeneity. clinically, there are two main groups of disorders, one dominated by muscle involvement, the other by brain dysfunction. biochemically, the enzyme defect may be confined to one or a few tissues (reflecting the existence of tissue-specific isozymes) or affect all tissues. immunologically reactive enzyme protein is decreased in some forms of cox deficiency but not in others. because cox is encod ... | 1988 | 2841309 |
| effect of parathyroid hormone and forskolin on cytoskeletal protein synthesis in cultured mouse osteoblastic cells. | parathyroid hormone (pth) has been shown to cause transient cell shape changes in bone cells. we have examined the effects of parathyroid hormone and forskolin on the organization and expression of cytoskeletal proteins in cultured mouse endosteal osteoblastic cells. analysis of [35s]methionine-labeled cytoskeletal proteins isolated on two-dimensional gel electrophoresis showed that pth treatment (24 h) stimulated the de novo biosynthesis of actin, vimentin and tubulins in confluent cells, where ... | 1988 | 2840972 |
| a new hamster model for adenoviral vaccination. | both adult and baby hamsters infected intranasally with human adenovirus type 5 exhibited virologic, serologic, and histologic evidence of infection. when 8-day old hamsters were infected with 4 x 10(6) pfu, concentrations of virus up to 2 x 10(6) pfu/animal were detected in the lung, peaking on day 2. the minimum infectious dose was 1 x 10(3) pfu/animal. this model may be useful in studies of conventional and recombinant adenoviral vaccines for humans. | 1988 | 2840876 |
| developmental changes in myosin isoforms from slow and fast latissimus dorsi muscles in the chicken. | in the course of muscle differentiation, changes in fibre-type population and in myosin composition occur. in this work, the expression of native myosin isoforms in developing fast-twitch (posterior latissimus dorsi; pld) and slow-tonic (anterior latissimus dorsi; ald) muscles of the chick was examined using electrophoresis under nondissociating conditions. the major isomyosin of 11-day-old embryonic pld comigrated with the adult fast myosin fm3. two additional components indistinguishable from ... | 1988 | 2840316 |
| selective inhibition of arachidonate 5-lipoxygenase by novel acetohydroxamic acids: biochemical assessment in vitro and ex vivo. | 1. the chemically novel acetohydroxamic acids, bw a4c, bw a137c and bw a797c, are potent inhibitors of the synthesis of leukotriene b4 (ltb4) from arachidonic acid by human leucocyte homogenates: the concentrations required for 50% inhibition (ic50) were 0.1 microm, 0.8 microm and 0.5 microm respectively. inhibition was less at higher concentrations of arachidonic acid. 2. these compounds also inhibited the synthesis of [14c]-5-hete from [14c]-arachidonic acid and the calcium-dependent synthesis ... | 1988 | 2840160 |
| cannabis consumption in catalonia, spain. | 2010 | 2840140 | |
| human and simian immunodeficiency retroviruses: activation and differential transactivation of gene expression. | new west african human immunodeficiency viruses (hiv-2s) and simian immunodeficiency virus (siv) contain functional transactivator (tat) gene and tat response elements. their long terminal repeats (ltr) and tat genes are more related among themselves than to hiv-1 ltr and tat gene. the viral gene expression of hiv-2 as well as siv can be stimulated by t cell activators, such as mitogens and phorbol esters. hiv-2 and siv display a much broader transactivation response specificity than does hiv-1. ... | 1988 | 2840105 |
| [role of the leukotrienes in asthma]. | leukotrienes (lt), which are derived from arachidonic acid and have similar chemical structures to the prostaglandins, have recently been shown to contain most of the biological activity previously attributed to "slow reacting substance of anaphylaxis" (srs-a). ltc4, ltd4 and lte4 have potent bronchoconstrictor actions. lte4 causes very long-lasting bronchoconstriction. ltb4 has inflammatory properties and possesses powerful chemotactic activity on leukocytes, especially neutrophil polymorphonuc ... | 2010 | 2840090 |
| [opioid peptides and their possible physiological significance]. | 2009 | 2839888 | |
| [biochemical aspect in cardiac ischemia--ischemic changes and reperfusion injury]. | 2010 | 2838873 | |
| dynamic liver scanning in cirrhosis. | dynamic hepatic scintigraphy was performed in 49 patients with established cirrhosis, using intravenous 99tcm-pertechnetate and 99tcm-sulphur colloid in a prospective study of its predictive value. there was a close correlation between the hepatic perfusion index (reflecting the ratio of arterial to total hepatic blood flow) obtained with pertechnetate (hpi-p) and with sulphur colloid (hpi-c) (r = 0.775; p less than 0.0001), and both indices correlated with disease severity (hpi-p p less than 0. ... | 2010 | 2838775 |
| evidence for a functional defect in the translocation of the methotrexate transport carrier in a methotrexate-resistant murine l1210 leukemia cell line. | properties of the methotrexate (mtx) transport carrier were examined in a stable single-step 16-fold mtx-resistant l1210 murine leukemia cell line with unchanged dihydrofolate reductase gene copy and thymidylate synthase and dihydrofolate reductase levels and activities. mtx influx was markedly depressed due to a decrease in vmax without a change in km. from this cell line a clonal variant with greater resistance to mtx was identified due solely to a further decrease in influx vmax. trans-stimul ... | 2006 | 2838483 |