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imported salmonella enteritidis cases: a multiphage outbreak among austrian vacationers in turkey, 2008.an outbreak of salmonella enteritidis infection among high school graduates was associated with the 2008 three-week summer splash event in turkey. a similar outbreak, caused by salmonella enteritidis pt6, affected 70 of 2879 graduates attending the 2007 summer splash event. a total of 103 cases of salmonella enteritidis were identified by passive and active case finding among the 8914 participants of the 2008 event. a case series investigation was performed. the cases originated from eight of th ...200919280141
a foodborne outbreak of salmonella enteritidis phage type 6 in austria, 2008.in april 2008, a general foodborne outbreak of salmonella enteritidis phage type 6 affected nine persons (two households in salzburg and one household in tyrol; eight microbiologically confirmed cases and one possible case). epidemiological investigation revealed that all cases had eaten lunch together at a farm. homemade bread dumpling loaf, prepared with eggs from the farm, was the most likely vehicle for the pathogen. six eggs were bacteriologically tested and yielded the outbreak strain on t ...200919280139
salmonellosis in austria: situation and trends.non-typhoidal salmonella spp. are a major cause of human gastroenteritis in many parts of the world. most of these infections are zoonotic and are transmitted from healthy carrier animals to humans through contaminated food. in austria we are facing an ongoing salmonellosis epidemic that started in 1989. the main cause of the epidemic is a massive increase of infections due to s. enterica subsp. enterica serovar enteritidis (s. enteritidis), a serotype prevalent in poultry, particularly in eggs. ...200919280133
growth inhibition of pathogenic bacteria and some yeasts by selected essential oils and survival of l. monocytogenes and c. albicans in apple-carrot juice.food safety is a fundamental concern of both consumers and the food industry. the increasing incidence of foodborne diseases increases the demand of using antimicrobials in foods. spices and plants are rich in essential oils and show inhibition activity against microorganisms, which are composed of many compounds. in this research, effects of garlic, bay, black pepper, origanum, orange, thyme, tea tree, mint, clove, and cumin essential oils on listeria monocytogenes aufe 39237, escherichia coli ...200919278342
antimicrobial susceptibilities, phage types, and molecular characterization of salmonella enterica serovar enteritidis from chickens and chicken meat in turkey.thirty-eight salmonella enteritidis isolates from chickens and chicken meat in turkey were examined for antimicrobial susceptibility, xbai pulsed-field gel electrophoresis (pfge) patterns, phage types, plasmid profiles, and resistance genes. seven different pfge patterns were observed, with the most common accounting for 71% (x1). the most common phage type was pt4, followed by pt7, pt16, pt1, pt6, and pt35. different phage types shared the same pfge pattern. twenty-one isolates were susceptible ...200919278340
genetic lines differ in toll-like receptor gene expression in spleens of chicks inoculated with salmonella enterica serovar enteritidis.toll-like receptors (tlr) recognize evolutionarily conserved molecular motifs (pathogen-associated molecular patterns) of infectious microbes and initiate innate immune response upon activation with relevant pathogens. this study investigated the acute effect of salmonella enteritidis challenge on tlr mrna expression in cecum and spleen of birds from 3 distinct genetic lines. chicks from broiler, leghorn, and fayoumi lines were inoculated or mock-inoculated with salmonella enteritidis. the mrna ...200919276417
bilateral periprosthetic joint infection caused by salmonella enterica serotype enteritidis, and identification of salmonella sp using molecular techniques.salmonella septic arthritis is rare. our objective was to identify bacterial species from joint fluid using a broad-range real-time pcr and pyrosequencing technique. we describe a case of bilateral salmonella enterica serotype enteritidis infection of right and left total knee arthroplasties. dna was extracted from the joint fluid of the left knee, amplified by pcr, and the amplicons were evaluated by pyrosequencing. the patient was treated with ciprofloxacin, and the polyethylene liners were re ...200919269872
food poisoning due to salmonella enteritidis--a case report.a male in his early seventies complained of abdominal pain and diarrhea at 7h after ingesting a small piece of gratin from a box lunch prepared by a caterer. he was admitted to a hospital, but died 37 h later. dozens of people who had eaten the same box lunch also complained of diarrhea. all of them recovered after medical treatment. a later investigation demonstrated salmonella enteritidis (se) in the gratin from the box lunch. an autopsy revealed very severe typhloenteritis with edema and subm ...200919269228
[microbiological contamination and antimicrobial activity of cristalised cane sugar on some medically important microorganisms in costa rica].microbiological contamination and antimicrobial activity of cristalised cane sugar on some medically important microorganisms in costa rica. unrefined cristalised cane sugar, obtained after the filtration and evaporation of sugar cane juice, is a nutritional product of traditional consumption in costa rica and other neotropical countries. it has been used in the topic treatment of infected wounds, with satisfactory results even with some antibiotic-ressistant bacteria. we studied the microbiolog ...200819256417
re-exposure to endotoxin induces differential cytokine gene expression in the rat hypothalamus and spleen.this study was designed to investigate whether the pattern of hypothalamic and splenic cytokine expression induced by peripheral administration of a bacterial lipopolysaccharide (lps) is affected by prior exposure to lps derived from another bacterial strain. injection of lps from salmonella enteritidis (lps(2)) alone resulted in increased hypothalamic gene expression of il-1beta, il-6, tnfalpha, il-1ra and il-10. however, pre-exposure to lps derived from escherichia coli (lps(1)) 3 weeks before ...200919254758
polyserositis due to salmonellaenterica serovar enteritidis.salmonella enterica serovar enteritidis (s. enteritidis), a non-typhoid salmonella, is an important emerging pathogen that usually causes gastroenteritis. here, we report polyserositis with right-sided pleural effusion and loculated collection of fluid in the peritoneum caused by s. enteritidis in a 60-year-old man from southern india. the patient was immunocompetent and did not have preceding gastroenteritis or any local structural abnormality. malnutrition and old age might have been the predi ...200919251293
salmonellosis cases caused by a rare salmonella enteritidis pt6c associated with travel to bulgaria, june-july 2008.in june 2008 an outbreak of gastroenteritis was registered in sunny beach resort situated on the black sea coast in bulgaria, affecting 14 employees of a hotel, five of whom tested positive for salmonella enteritidis. during june-july 2008 four sporadic s. enteritidis cases were also reported and two of them were foreign tourists. in the same period s. enteritidis cases connected with travel to bulgaria were reported to the european centre for disease prevention and control (ecdc) from finland, ...200919250623
functional properties of novel protective lactic acid bacteria and application in raw chicken meat against listeria monocytogenes and salmonella enteritidis.in this study 635 lactic acid bacteria of food origin were evaluated for their potential application as protective cultures in foods. a stepwise selection method was used to obtain the most appropriate strains for application as protective cultures in chicken meat. specifically, all strains were examined for antimicrobial activity against various gram positive and gram negative pathogenic and spoilage bacteria. strains exhibiting anti-bacterial activity were subsequently examined for survival in ...200919249112
antibiotic-resistant salmonella and escherichia coli isolates with integrons and extended-spectrum beta-lactamases in surface water and sympatric black-headed gulls.to examine surface water from a pond in the northeastern part of the czech republic and young black-headed gulls (larus ridibundus) nesting on the same pond for the presence of antibiotic-resistant salmonella and escherichia coli.200919245407
faecal sampling underestimates the actual prevalence of salmonella in laying hen flocks.in all european union member states, salmonella monitoring in poultry flocks is obligatory. in these monitoring programmes, a limited number of pooled faeces and/or dust samples are collected to determine whether salmonella is present in the flocks or not. whether these limited sampling protocols are sufficiently sensitive to detect expected low within-flock prevalences of an intermittently shed pathogen is not yet clear. in this study, a comparison is made between different sampling procedures ...200919243563
epidemiological data on food poisonings in japan focused on salmonella, 1998-2004.in japan, the numbers and cases of food poisonings must be reported as required by the food sanitation law. this paper focuses on salmonella, one of the leading food-borne pathogens in japan, and it analyses the reported food poisoning data to assess the nature of salmonella-associated food-borne disease. obviously, these data do not exactly reflect the burden of food-borne illness associated with salmonella; however, trends in salmonella food poisoning and implicated foods could be identified f ...200819238616
risk factors for salmonella enterica subsp. enterica contamination in 519 french laying hen flocks at the end of the laying period.a cross-sectional study was carried out to identify risk factors for salmonella spp. contamination in french laying hen flocks at the end of the laying period. five hundred and nineteen flocks were studied between october 2004 and september 2005. the salmonella status of the flocks was assessed from 5 faeces samples (pooled faeces samples from cage flocks and foot swabs from flocks kept on the floor, i.e. in a barn, outdoors and on organic farms) and 2 dust samples analysed using a classical bac ...200919237216
coordinated regulation of virulence during systemic infection of salmonella enterica serovar typhimurium.to cause a systemic infection, salmonella must respond to many environmental cues during mouse infection and express specific subsets of genes in a temporal and spatial manner, but the regulatory pathways are poorly established. to unravel how micro-environmental signals are processed and integrated into coordinated action, we constructed in-frame non-polar deletions of 83 regulators inferred to play a role in salmonella enteriditis typhimurium (stm) virulence and tested them in three virulence ...200919229334
diseases in chicks and laying hens during the first 12 years after battery cages were banned in switzerland.between 1992 and 2003, a period of 12 years after the definitive ban on battery cages in switzerland, more than 10,000 replacement chicks and laying hens were examined postmortem. there was a significant decrease in the incidence of viral diseases, mostly due to a reduction in marek's disease, but there was a marked increase in bacterial diseases, particularly since 1999, mainly due to colisepticaemia in young laying hens. there was a steady decrease in parasitic infections, but the incidence of ...200919218590
a comparison of two pcr-based typing methods with pulsed-field gel electrophoresis in salmonella enterica serovar enteritidis.two novel molecular typing methods, multiple-locus variable-number tandem repeats (vntr) analysis (mlva) and multiple amplification of phage loci typing (maplt), were compared with pulsed-field gel electrophoresis (pfge) for the discrimination of 128 salmonella enterica serovar enteritidis (s. enteritidis) isolates. selected epidemiologically unrelated isolates represented a cross-section of phage types routinely isolated in australia and included 28 isolates that could not be assigned a phage t ...200919217348
delineation of the chemomechanosensory regulation of gastrin secretion using pure rodent g cells.gastrin is a key regulator of gastric acid secretion. we aimed to isolate pure g cells to identify the mechanistic basis of luminal- and strain-mediated regulation.200919208342
mechanisms of egg contamination by salmonella enteritidis.salmonella enteritidis (se) has been the major cause of the food-borne salmonellosis pandemic in humans over the last 20 years, during which contaminated hen's eggs were the most important vehicle of the infection. eggs can be contaminated on the outer shell surface and internally. internal contamination can be the result of penetration through the eggshell or by direct contamination of egg contents before oviposition, originating from infection of the reproductive organs. once inside the egg, t ...200919207743
the poultry red mite (dermanyssus gallinae): a potential vector of pathogenic agents.the poultry red mite, d. gallinae has been involved in the transmission of many pathogenic agents, responsible for serious diseases both in animals and humans. nowadays, few effective methods are available to control the ectoparasite in poultry farms. consequently, this is an emerging problem which must be taken into account to maintain good health in commercial egg production. this paper addresses the vector capacity of the ectoparasite with special emphasis on salmonellae, pathogenic agents re ...200919205905
expression profile of toll-like receptors within the gastrointestinal tract of 2-day-old salmonella enteriditis-infected broiler chickens.salmonella enterica serovar enteriditis (se) causes a majority of foodborne illness in the u.s. a more productive avian innate immune response could reduce bacterial colonization and the incidence of infection in humans. however, quantification and comparison of the toll-like receptors (tlr), a component of the innate immune system that recognize bacterial pathogens, and their response to se colonization across the avian gastrointestinal (gi) tract has not been reported. therefore, we assessed t ...200919201111
molecular characterization of antimicrobial resistance in salmonella isolated from animals in japan.aims: to investigate the prevalence of integrons and antimicrobial resistance genes in salmonella recovered from animals in japan. methods and results: forty-eight out of ninety-four (51.1%) salmonella isolates showed multidrug resistance phenotypes and harboured at least one antimicrobial resistance gene. twenty-two out of forty-seven (46.8%) salmonella enterica serovar typhimurium that were multidrug-resistant were of definitive phage type dt104. class 1 integrons were identified in 34/94 isol ...200919200308
antimicrobial efficacy of zinc oxide quantum dots against listeria monocytogenes, salmonella enteritidis, and escherichia coli o157:h7.zinc oxide quantum dots (zno qds) are nanoparticles of purified powdered zno. these were evaluated for antimicrobial activity against listeria monocytogenes, salmonella enteritidis, and escherichia coli o157:h7. the zno qds were utilized as a powder, bound in a polystyrene film (zno-ps), or suspended in a polyvinylprolidone gel (zno-pvp). bacteria cultures were inoculated into culture media or liquid egg white (lew) and incubated at 22 degrees c. the inhibitory efficacies of zno qds against 3 pa ...200919200107
chicken heterophils are recruited to the site of salmonella infection and release antibacterial mature cathelicidin-2 upon stimulation with lps.the biological functions of avian cathelicidins are poorly defined. in mammals, cathelicidins have shown to possess potent broad-range antimicrobial activity as well as immunomodulatory activities. therefore, we investigated the microbicidal activities and localization of cathelicidin-2 in non-infected and salmonella-challenged broiler chickens. using immunohistochemistry, cathelicidin-2 was shown to be abundantly present in heterophils, localized in the large rod-shaped granules, but absent in ...200919187966
importance of subunit vaccine antigen of major fli c antigenic site of salmonella enteritidis ii: a challenge trial.salmonella enterica subsp. enterica serovar enteritidis (se) infection in chickens shows a mild pathogenicity except for young ages, compared with other animals, and laying hens sometimes produce se-contaminated eggs leading to public health concerns. to reduce the problem, se bacterin in poultry farms has been applied. we previously demonstrated that a subunit antigen, g.m. part polypeptide in se-fli c (sep 9), could be a candidate subunit antigen of se vaccine which may show less side effects ...200919186198
efficiency of slightly acidic electrolyzed water for inactivation of salmonella enteritidis and its contaminated shell eggs.the efficiency of slightly acidic electrolyzed water (saew) at different temperatures (4, 20 and 45 degrees c) for inactivation of salmonella enteritidis and it on the surface of shell eggs was evaluated. the bactericidal activity of saew, sodium hypochlorite solution (naclo) and acidic electrolyzed water (aew) to inactivate s. enteritidis was also compared. saew with a ph value of 6.0-6.5 used was generated by the electrolysis of a dilute hydrochloric acid (2.4 mm) in a chamber without a membra ...200919185376
further studies on vertical transmission and persistence of salmonella enterica serovar enteritidis phage type 4 in chickens.one-week-old commercial layers were infected orally with 10(8) colony forming units of salmonella enterica serovar enteritidis phage type 4. no mortality was observed. the inoculated organism was isolated in decreasing viable numbers from a number of tissues, particularly the spleen, liver and caeca. organisms present in the spleen were primarily localized within macrophages. no salmonella enteritidis organisms were isolated between 10 and 24 weeks of age, when the experiment was terminated afte ...200119184915
pathogenicity of salmonella enteritidis phage types 4, 8 and 23 in specific pathogen free chicks.the pathogenicity of two isolates of salmonella enterica serovar enteritidis (se) phage type (pt) 4, three of pt8 and one of pt23 was investigated in groups of 1-day-old specific pathogen free white leghorn chicks. two groups were crop gavaged with each culture but at two different doses. two additional groups were given salmonella enterica serovar pullorum (sp) at similar doses and one further group served as uninoculated controls. body weights were recorded at 14, 21, and 28 days postinoculati ...200019184855
evaluation of the effect of mannan-oligosaccharides on the competitive exclusion of salmonella enteritidis colonization in broiler chicks.this study evaluated the protection against colonization by salmonella enterica serovar enteritidis in the chick's caecum when dosed with caecal contents from hens fed dietary carbohydrates based on mannose. protection was investigated by comparing the effects of four suspensions of hen caecal contents (hcc) fed diets supplemented with 2.5% d -mannose (man-hcc), 2.5% mannoseoligosaccharide (mos-hcc), 2.5% palm kernel meal (pkm-hcc) or unsupplemented mash (mashhcc). four trials to quantify and co ...200019184854
contribution of fimbriae and flagella of salmonella enteritidis to colonization and invasion of chicks.isogenic mutants of salmonella enteritidis defective for the elaboration of fimbrial types sef14, sef17, sef21 and flagella were used to study the contribution these organelles made to colonization, invasion and lateral transfer in young chicks. the caecum, liver and spleen were colonized within 24 h following oral inoculation of 1-day-old chicks with 10(5) wild-type s. enteritidis strain la5. however, for some mutants, the numbers of organisms recovered from internal organs was reduced signific ...200019184818
longevity of augmented phagocytic activity of heterophils in neonatal chickens following administration of salmonella enteritidis-immune lymphokines to chickens.previously, our laboratory reported an existing relationship between ontogenesis of heterophil activity and susceptibility to salmonella enteritidis infection in poultry during the first week post-hatch. the prophylactic administration of s. enteritidis-immune lymphokines has been shown to enhance heterophil function in vitro and reduce s. enteritidis organ invasion in 1-day-old chicks. however, how long the heterophils remain activated is currently unknown. the objective of this research was to ...200019184797
analysis of foodborne outbreak data reported internationally for source attribution.analysis of foodborne outbreak data is one approach to estimate the proportion of human cases of specific enteric diseases attributable to a specific food item (food attribution). although we recognize that for a variety of reasons reported outbreaks represent only a small portion of all actual outbreaks, using outbreak data for food attribution is the only methodological approach where, theoretically, there is an actual direct link between the pathogen, its source and each infected person. the ...200919178974
unprecedented hemiketal cembranolides with anti-inflammatory activity from the soft coral lobophytum durum.chemical investigations of the soft coral lobophytum durum have led to the isolation of three unprecedented hemiketal cembranolides, durumhemiketalolides a-c (1-3). full nmr spectroscopic assignments are provided for all isolated metabolites. the anti-inflammatory activities of 1-3 were evaluated in vitro.200919177635
detection of living salmonella cells using bioluminescence.atp-based bioluminescence using mutant firefly luciferase was combined with an immunochromatographic lateral flow test strip assay for salmonella enteritidis detection. in this combination method, the salmonella-antibody-gold complex captured at the test line on the test strip was lysed by heat-treatment, and the atp released from the cells was measured using mutant luciferase. this method resulted in approximately 1,000 times higher sensitivity in the detection of salmonella (i.e. 10(3) c.f.u./ ...200919169891
cellular assessment of crop lymphoid tissue from specific-pathogen-free white leghorn chickens after salmonella enteritidis challenge.the crop may be an important site along the upper alimentary tract in which a humoral immune response against salmonella enteritidis (se) is elicited locally. the mucosal immune response within the crop (ingluvies) of specific-pathogen-free (spf) white leghorn (wl) chickens against se was investigated. three trials were conducted using spf wl pullets at age 5-6 wk. trial 1 consisted of 77 birds evaluated for 10 wk post-se infection (pi), trial 2 was composed of 72 birds monitored through 8 wk pi ...200819166059
passive immunity of progeny from broiler breeders vaccinated with oil-emulsion bacterin against salmonella enteritidis.young poultry are very susceptible to salmonella enteritidis (se) infections because of the absence of complete intestinal flora colonization and an immature immune system. this study evaluated the role of passive immunity on the resistance of young birds against early infections caused by se. the progeny of broiler breeders vaccinated with an oil-emulsion bacterin was compared to the progeny of unvaccinated birds. efficacy was determined by challenging birds at 1 and 14 days of age with se nal ...200819166046
ileal mucosal and fecal pancreatitis associated protein levels reflect severity of salmonella infection in rats.microbial infections induce ileal pancreatitis-associated protein/regenerating gene iii (pap/regiii) mrna expression. despite increasing interest, little is known about the pap/regiii protein. therefore, ileal mucosal pap/regiii protein expression, localization, and fecal excretion were studied in rats upon salmonella infection.200919160051
biofilm development capacity of salmonella strains isolated in poultry risk factors and their resistance against disinfectants.prevention of salmonella contamination of poultry products requires detailed knowledge of the most important risk factors associated with its presence in the production system. the aim of this study was to identify the risk factors for salmonella contamination in 44 broiler and 51 layer farms and determine the biofilm development capacity of the strains isolated. then, glutaraldehyde (50% vol/vol), formaldehyde (37% vol/vol), and hydroxide peroxide (35% vol/vol) were applied to evaluate their ca ...200919151358
evaluation of the bacterial diversity in cecal contents of laying hens fed various molting diets by using bacterial tag-encoded flx amplicon pyrosequencing.laying hens are typically induced to molt to begin a new egg-laying cycle by withdrawing feed for up to 12 to 14 d. fasted hens are more susceptible to colonization and tissue invasion by salmonella enterica serovar enteritidis. much of this increased incidence in fasted hens is thought to be due to changes in the native intestinal microflora. an alternative to feed withdrawal involves feeding alfalfa meal crumble to hens, which is indigestible by poultry but provides fermentable substrate to th ...200919151343
dahi containing probiotic lactobacillus acidophilus and lactobacillus casei has a protective effect against salmonella enteritidis infection in mice.salmonella enteritidis infection has received attention during recent years owing to its high prevalence worldwide. in the present study, the protective effect of probiotic dahi (curd) supplemented with lactobacillus acidophilus and l. casei against salmonella enteritidis infection in mice is investigated. seven days pre-feeding with probiotic dahi significantly increased anti-s. enteritidis siga (secretary iga) antibodies and lymphocyte proliferation in s. enteritidis infected mice. il-2, il-6 ...200819144289
extensive salmonella enteritidis endocarditis involving mitral, tricuspid valves, aortic root and right ventricular wall.after conducting a pubmed search, 11 articles describing a total of 12 cases of native valve salmonella enteritidis (s. enteritidis) endocarditis were identified in the english literature. only 13 cases of prosthetic valve endocarditis attributed to s. enteritidis have been published in the english literature. only 1 case involving the myocardium and other valves concomitantly could be located. transthoracic echocardiography proved inadequate to demonstrate valvular vegetations. transesophageal ...200919135858
use of pulsed-field gel electrophoresis to characterize the heterogeneity and clonality of salmonella serotype enteritidis, typhimurium and infantis isolates obtained from whole liquid eggs.salmonella is a well-documented pathogen known to occur in a wide range of foods, especially poultry products. the most frequently reported food-sources of human infection are eggs and egg products. in this study, in order to describe salmonella contamination of egg products, 144 liquid egg samples were collected from 3 different egg-breaking plants during the 3 sampling periods. salmonella detection was performed on raw samples stored at 2 degrees c for 2 days (d+2) and on pasteurised samples s ...200919128850
phage type and antimicrobial susceptibility of salmonella enterica serovar enteritidis from food-producing animals in japan between 1976 and 2004.a total of 56 isolates of salmonella enterica serovar enteritidis, including 38 isolates from poultry, 16 from cattle and two from pigs, collected between 1976 and 2004, were subjected to bacteriophage typing and antimicrobial susceptibility testing. phage type (pt) 8 was predominant in bovine isolates, whereas pt1 and pt4 were predominant in poultry isolates. resistance was found for 8 of 11 antimicrobials tested, at the following rates: 46.4% for dihydrostreptomycin followed by ampicillin and ...200819123313
comparison of environmental sampling methods for detecting salmonella in commercial laying flocks in the uk.to investigate the performance of the salmonella national control programme (ncp) sampling/testing methods in laying flocks of domestic fowl.200819120919
multiple regulatory pathways associated with high-level ciprofloxacin and multidrug resistance in salmonella enterica serovar enteritidis: involvement of rama and other global regulators.mechanisms of antibiotic resistance were examined in nalidixic acid-resistant salmonella enterica serovar enteritidis field isolates displaying decreased susceptibility to ciprofloxacin and in in vitro-derived ciprofloxacin-resistant mutants (104-cip and 5408-cip). all field isolates harbored a single gyra mutation (d87y). deletion of acrb and complementation with wild-type gyra increased quinolone susceptibility. selection for ciprofloxacin resistance was associated with the development of an a ...200919104017
a salmonella infection complicated with suppurative thyroiditis and ruptured aortic mycotic aneurysm in a renal transplant recipient.we report a renal transplant recipient who presented with fever and chills for 2 days. the blood and stool cultures revealed the growth of salmonella enteriditis. a whole-body gallium scan played an important role in the subsequent diagnosis of suppurative thyroiditis. to our knowledge, this is the first report of acute s. enteriditis thyroiditis in a renal transplant recipient. despite vigorous antibiotic use and a partial thyroidectomy, he experienced recurrent s. enteriditis infection, result ...200819100483
are host characteristics or exposure factors mainly involved in the acquisition of zoonotic salmonella and campylobacter coinfection in humans?we hypothesized that patients coinfected with zoonotic salmonella and campylobacter were frailer than monoinfected salmonella or campylobacter patients. the study cohort included all first-time salmonella/campylobacter infections in aarhus and north jutland counties, denmark, from 1991 through 2003. data on comorbidity, hospitalization in relation to the salmonella/campylobacter infection, and 1-year mortality were obtained from electronic registries. among 13,449 individuals, 114 (0.85%) had sa ...200919099355
gas-forming parotid abscess in a diabetic patient: an unusual complication of salmonella enteritidis bacteremia.parotid abscess, not only a focal infection but also a sequela of systemic infection, necessitates the detailed search for the underlying pathogens. a 76-year-old diabetic male visited the emergency room of a hospital because of intermittently low-grade fever and a painful lump in the right parotid gland region for 7 days. progressive painful swelling of the gland with the signs of severe sepsis developed 3 days later. computed tomography of the neck revealed a cavitary lesion with an air-fluid ...200819092325
analysis of the role of 13 major fimbrial subunits in colonisation of the chicken intestines by salmonella enterica serovar enteritidis reveals a role for a novel locus.salmonella enterica is a facultative intracellular pathogen of worldwide importance. over 2,500 serovars exist and infections in humans and animals may produce a spectrum of symptoms from enteritis to typhoid depending on serovar- and host-specific factors. s. enteritidis is the most prevalent non-typhoidal serovar isolated from humans with acute diarrhoeal illness in many countries. human infections are frequently associated with direct or indirect contact with contaminated poultry meat or eggs ...200819091138
molecular study of salmonella enteritidis in poultry samples by pcr, plasmid curing, antibiotic resistance and protein pattern analysis.in this study two control isolates of salmonella enteritidis, rtcc1623 and rtcc1624, were obtained from the institute ofrazi (karaj-iran) and 14 strains were isolated from poultry samples in kermanshah province of iran, according to a standard protocol. these isolates were confirmed by pcr amplification of sefa gene fragments. results showed that, 6 isolates of 14 isolates of salmonella which their biochemical tests were positive contain 511 bp amplified fragments of the sefa gene. in other purp ...200719086500
characterization of bacteriophages used in the salmonella enterica serovar enteritidis phage-typing scheme.the 16 salmonella enterica serovar enteritidis (s. enteritidis) typing phages (setps) used in the laboratory of enteric pathogens (health protection agency, london, uk) phage-typing scheme have not previously been characterized in detail. we have examined the adsorption properties of the phages with respect to a number of s. enterica serovars and defined phage morphology with electron microscopy. pfge was used to estimate overall genome size and banding patterns generated by electrophoresis foll ...200919074657
salmonella in broiler flocks in the republic of ireland.in order to obtain an estimation of the prevalence of salmonella spp. in flocks of broilers in the republic of ireland, a study was conducted in 2006 in a total of 362 broiler flocks associated with four integrated companies. salmonella spp. was isolated from 27.3% of flocks, and eight salmonella serovars were identified, none of which were salmonella enteritidis or salmonella typhimurium. the most prevalent serovar was salmonella mbandaka, followed by salmonella kentucky, which respectively acc ...200919061369
severely obese have greater lps-stimulated tnf-alpha production than normal weight african-american women.obesity is associated with an increase in chronic, low-grade inflammation which has been implicated in the development of type 2 diabetes mellitus and cardiovascular disease. the purpose of this study was to determine whether obesity was associated with an elevation of whole blood lipopolysaccharide (lps)-stimulated tumor necrosis factor-alpha (tnf-alpha) production. african-american women were recruited from a larger study and assigned to one of five groups based on bmi: normal weight (norm; bm ...200919057521
aroa and arod mutations influence biofilm formation in salmonella enteritidis.aro mutants of salmonella enterica are frequently used as live vaccines for the oral vaccination of domestic animals. interestingly, besides their auxotrophy, they appear to be of reduced resistance to the components of innate immune response due to a defect in outer membrane and/or cell wall integrity. because different extracellular structures associated with the cell wall or outer membrane are involved in biofilm formation, we were interested in the ability of aroa and arod mutants of s. ente ...200919054077
in vitro activity of methyl gallate isolated from galla rhois alone and in combination with ciprofloxacin against clinical isolates of salmonella.salmonella remains a primary cause of food poisoning worldwide, and massive outbreaks have been witnessed in recent years. therefore, this study investigated the antimicrobial activity of methyl gallate (mg), which exhibited good antibacterial activity (mic=3.9-125 mg/ml) against all the bacterial strains tested. in a checkerboard dilution test, mg markedly lowered the mics of ciprofloxacin (cpfx) against salmonella. the combined activity of cpfx and mg against salmonella resulted in fractional ...200819047831
public health assessment of salmonella enterica serovar enteritidis inactivated-vaccine treatment in layer flocks.although there have been several reports on the efficacy assessment of a salmonella enterica serovar enteritidis vaccine against intestinal and parenchymatous organ diseases of laying hens, no public health risk characterization of its long-term effect on eggs has been reported. in this study, we attempted to assess the public health effect of an inactivated s. enterica serovar enteritidis vaccine against serovar enteritidis contamination of chicken eggs. we analyzed serovar enteritidis isolatio ...200919047380
identification of salmonella serotypes isolated from cantaloupe and chile pepper production systems in mexico by pcr-restriction fragment length polymorphism.a study was conducted in 2006 to determine the prevalence of salmonella on three cantaloupe farms in matamoros, coahuila, mexico, and on one farm that cultivates chile peppers var. bell in culiacán, sinaloa, mexico. samples from cantaloupe farms consisted of cantaloupe rinses, irrigation water, water from furrows in the field, and workers' hands. samples from the chile pepper farm consisted of rinses of chile peppers obtained at the field, pepper rinses obtained at the packing house, and irrigat ...200819044264
[the serological prevalence of salmonella enteritidis amongst tunja's canine population (a large colombian city)].determining the serological prevalence of salmonella enteritidis in the city of tunja's canine population (in boyacá) from july to october 2006 and establishing the relationship between predisposed factors and seroreactivity within this canine population.200819043638
survey of the prevalence of salmonella on commercial broiler farms in the united kingdom, 2005/06.between october 2005 and september 2006, all european union member states were required to carry out standardised surveys of the prevalence of salmonella in broiler flock holdings to establish baseline data from which to derive national targets for disease reduction. in the uk 382 holdings were sampled, 41 of which were positive for salmonella, giving an estimated weighted prevalence of 10.7 per cent (95 per cent confidence interval [ci] 8.1 to 13.1 per cent). the serotype most frequently isolat ...200819043089
prevalence, characterisation and antimicrobial resistance of campylobacter and salmonella in raw poultrymeat in the uk, 2003-2005.this study was conducted to determine the prevalence and antimicrobial resistance of campylobacter and salmonella isolates from retail poultrymeat in the uk during 2003-2005. poultrymeat (n = 2104) were more frequently contaminated with campylobacter (57.3%) than with salmonella (6.6%). chicken exhibited the highest contamination from campylobacter (60.9%), followed by duck (50.7%), turkey (33.7%) and other poultrymeat (34.2%). duck had the highest contamination from salmonella (29.9%), compared ...200819031145
sublethal infection with salmonella enteritidis by the natural route induces intestinal and joint inflammation in mice.reactive arthritis (rea) is a sterile inflammation triggered by a distal mucosal infection, which suggests a contribution from bacterial products. investigation on the pathogenesis of rea is difficult because of the limited studies that can be performed in humans; therefore the availability of animal models is crucial. we hereby describe a murine model for studying the early stages of salmonella-induced rea. balb/c mice infected by the natural route with a sublethal dose of s. enteritidis showed ...200919022393
in ovo treatment with cpg oligodeoxynucleotides decreases colonization of salmonella enteriditis in broiler chickens.induction of the innate immune response in newly hatched chickens is important for limiting infections with bacteria, such as salmonella enterica serovar enteriditis (se). cpg oligodeoxynucleotides (cpg-odn) can stimulate the innate immune response of young chickens. therefore, we examined the effectiveness of cpg-odn administered in ovo on intestinal colonization by se and the ability to modulate the function of heterophils in young chickens. heterophils were isolated from 2-day-old chickens an ...200919019459
fractional differential equations based modeling of microbial survival and growth curves: model development and experimental validation.a fractional differential equations (fdes)-based theory involving 1- and 2-term equations was developed to predict the nonlinear survival and growth curves of foodborne pathogens. it is interesting to note that the solution of 1-term fde leads to the weibull model. nonlinear regression (gauss-newton method) was performed to calculate the parameters of the 1-term and 2-term fdes. the experimental inactivation data of salmonella cocktail in ground turkey breast, ground turkey thigh, and pork shoul ...200819019113
divercin v41 from gene characterization to food applications: 1998-2008, a decade of solved and unsolved questions.the emergence of an increasing number of antibiotic resistant human clinical bacteria has been a great cause of concern for the last decades. as an example, staphylococcus aureus isolates in the hospital environment are becoming more and more resistant to antibiotics including vancomycin which is considered as a last line of defence in treatment of staphylococcus aureus-resistant methicillin. on the other hand, food safety is threatened by development of pathogenic bacteria including listeria mo ...200919018960
protective immunity to systemic infection with attenuated salmonella enterica serovar enteritidis in the absence of il-12 is associated with il-23-dependent il-22, but not il-17.il-12 is essential for protective t cell-mediated immunity against salmonella infection. to characterize the role of the related cytokine il-23, wild-type (wt) c57bl/6 and p19(-/-) mice were infected systemically with an attenuated strain of salmonella enterica serovar enteritidis (s. enteritidis). il-23-deficient mice controlled infection with s. enteritidis similarly as wt mice. similar ifn-gamma production as compared with wt mice, but defective il-17a and il-22 production was found in the ab ...200819017979
persistence and clearance of different salmonella serovars in buildings housing laying hens.we investigated factors associated with persistence of different salmonella serovars in buildings housing laying hens in great britain using survival analysis. a total of 264 incidents of salmonella detection occurring between july 1998 and august 2007 in 152 houses were recorded. for incidents involving salmonella enteritidis (se), both the rodent score of the house and the type of house were positively associated with persistence. for non-se serovars, only the type of house was associated with ...200919017427
[protracted nosocomial outbreak of salmonella enteritidis lt 8/7].in spring 2007a cluster of nosocomial salmonellosis cases, culture confirmed for salmonella enteritidis lysotype (lt) 8/7, occurred in a wolfsburg hospital. an outbreak investigation was initiated to determine the epidemiology of the outbreak and to identify and control the possible sources.200819012198
acalculous cholecystitis due to salmonella enteritidis.acute acalculous cholecystitis (aac) is defined as an acute inflammation of the gallbladder in the absence of stones. we herein report a case of a young man who developed aac after a salmonella enteritidis gastrointestinal infection.200819009660
ceramide and cerebrosides from the octocoral sarcophyton ehrenbergi.chemical investigation of the octocoral sarcophyton ehrenbergi, collected at the dongsha islands, taiwan, has led to the isolation of a known ceramide (1) and two new cerebrosides, sarcoehrenosides a (2) and b (4), along with three known cerebrosides (3, 5, and 6). the structures of the new compounds were established by spectroscopic and chemical methods. sarcoehrenoside a (2) differs from previously known marine cerebrosides in that it possesses a rare alpha-glucose moiety. compounds 1-6 were e ...200919006372
differential mrna expression of the avian-specific toll-like receptor 15 between heterophils from salmonella-susceptible and -resistant chickens.pattern recognition receptors (prrs) are essential for recognition of conserved molecular constituents found on infectious microbes. toll-like receptors (tlrs) are a critical component of the prr repertoire and are coupled to downstream production of cytokines, chemokines, and antimicrobial peptides by tlr adaptor proteins. our laboratory previously demonstrated a role for tlr function in the differential innate response of two lines of chickens to bacterial infections. the aim of the present st ...200919002681
evaluation of a multiplex pcr system for simultaneous detection of salmonella spp., listeria monocytogenes, and escherichia coli o157:h7 in foods and in food subjected to freezing.conventional culture methods were compared to a multiplex polymerase chain reaction (pcr) assay for simultaneous detection of salmonella spp., listeria monocytogenes, and escherichia coli o157:h7 from enrichment cultures of various types of artificially inoculated and naturally contaminated foods. the multiplex pcr assay was evaluated in 44 types of spiked food samples, including meat, produce, fish, and dairy products targeting genes specific for each pathogen for simultaneous detection. the se ...200918991547
gene expression profiling in chicken heterophils with salmonella enteritidis stimulation using a chicken 44 k agilent microarray.salmonella enterica serovar enteritidis (se) is one of the most common food-borne pathogens that cause human salmonellosis and usually results from the consumption of contaminated poultry products. the mechanism of se resistance in chickens remains largely unknown. previously, heterophils isolated from broilers with different genetic backgrounds (se-resistant [line a] and -susceptible [line b]) have been shown to be important in defending against se infections. to dissect the interplay between h ...200818990222
the salmonella enteritidis lipopolysaccharide biosynthesis gene rfbh is required for survival in egg albumen.salmonella enteritidis is still a major cause of human food borne infections and can be associated with the consumption of meat and chicken eggs. it is the world's most common cause of salmonellosis in part because it has the ability to colonize the oviduct and contaminate eggs. it was shown that when stored at room temperature, s. enteritidis bacteria can multiply extensively in contaminated eggs. using the in vivo expression technology, it was shown that the rfbh gene, involved in lipopolysacc ...200918990194
characteristics of persistent salmonella enteritidis strains in two integrated broiler chicken operations of korea.the objectives of the study were to investigate the phenotypic and genotypic characterization of the persistent salmonella enteritidis (s. enteritidis) isolates in two integrated broiler chicken operations, with attention focused mainly on the epidemiological approach. in the distribution of virulence genes, salmonella enterotoxin (stn), invading host cell (inva), and salmonella plasmid virulence (spvc) genes were widely distributed among the s. enteritidis irrespective of their source of isolat ...200818981657
increased prescription rate of antibiotics prior to non-typhoid salmonella infections: a one-year nested case-control study.we conducted a nested case-control study of antibiotic consumption 1 y prior to a non-typhoid salmonella (nts) infection. we included all 1882 nts patients diagnosed in 1994-2003 in north jutland county, denmark, and for each case we retrieved 10 controls among all residents in the county, matched for the specimen date, gender, and age. antibiotic redemptions were retrieved from a countywide prescription database. being a marker of the infection, redemptions within 1 week before nts infection we ...200818979601
antiproliferative and antimicrobial activity of traditional kombucha and satureja montana l. kombucha.to carry out a preliminary investigation of the biological activity of kombucha beverages from camellia sinensis l. (black tea) and satureja montana l. (winter savory tea), that have consuming acidity.200818979556
survey on salmonella prevalence in slaughter pigs from saskatchewan.a study on slaughter pigs from saskatchewan detected salmonella organisms in 12.5% and 5.2% of cecal content and ileocaecal lymph node samples, respectively. cecal content prevalence was associated with larger farms and longer lairage periods. antimicrobial resistance was detected in 41.5% of the isolates. salmonella enteritidis was the second most prevalent serotype.200818978974
toll-like receptor 2 variants are associated with acute reactive arthritis.we previously reported a recent outbreak of salmonellosis in which some individuals developed complications of the enteric infection. the objective of this study was to identify genetic variants that might predispose infected individuals to develop articular and/or extraarticular sequelae after salmonella enteritidis infection.200818975326
prolonged fever in hiv disease due to salmonella enetritidis. 200818974506
chinese patients with defective il-12/23-interferon-gamma circuit in taiwan: partial dominant interferon-gamma receptor 1 mutation presenting as cutaneous granuloma and il-12 receptor beta1 mutation as pneumatocele.il-12/23-interferon-gamma circuit enhances reactive oxygen species (ros) synthesis in macrophage to attack intracellular pathogens such as mycobacteria and salmonella. defective ros in patients with chronic granulomatous disease (cgd) have increased susceptibility to these pathogens. however, patients with defective il-12/23-interferon-gamma circuit rather than cgd are not recognized in taiwan, endemic for tuberculosis and salmonella.200918972195
piezoelectric crystal for sensing bacteria by immobilizing antibodies on divinylsulphone activated poly-m-aminophenol film.a polymer sorbent formed at the surface of gold-plated piezoelectric crystal by anodic oxidation of m-aminophenol was investigated by voltammetric, ftir techniques for antibody coupling after activation by divinylsulphone. a novel film with increased capacity for immobilizing antibodies was obtained using phloroglucinol to modify poly-m-aminophenol via divinylsulphone. compared with the dip-coating methods using polyethylenimine and (gamma-aminopropyl)trimethoxysilane, this new technique gave mo ...200018967847
emergence of qnr determinants in human salmonella isolates in taiwan.the aim of this study was to determine the prevalence and characteristics of qnr-carrying salmonella isolates from humans in southern taiwan.200818957397
development of liposome immunoassay for salmonella spp. using immunomagnetic separation and immunoliposome.the ability to detect salmonella spp. is essential in the prevention of foodborne illness. this study examined a salmonella spp. detection method involving the application of immunomagnetic separation and immunoliposomes (ims/il) encapsulating sulforhodamine b (srb), a fluorescent dye. a quantitative assay was conducted by measuring the fluorescence intensity of srb that was produced from an immunomagnetic bead-salmonella spp.-immunoliposome complex. the results indicated detection limits of 2.7 ...200818955821
distribution trends of salmonella serovars in india (2001-2005).a total of 3079 samples were received and identified at the national salmonella and escherichia centre (nsec), central research institute, kasauli, india during 2001-2005. out of these, 2098 samples were from humans, 250 from animals, and 726 from meat, vegetables, seafood and the environment. the salmonella strains isolated were distributed among 35 different salmonella serovars. the most common serovars from humans were salmonella typhi (73%) and salmonella paratyphi a (24%) among typhoidal se ...200918950825
outbreaks of salmonellosis in minnesota (1998 through 2006) associated with frozen, microwaveable, breaded, stuffed chicken products.from 1998 through 2006, four outbreaks of salmonellosis associated with raw, frozen, microwaveable, breaded, prebrowned, stuffed chicken products were identified in minnesota. in 1998, 33 salmonella typhimurium cases were associated with a single brand of chicken kiev. in 2005, four salmonella heidelberg cases were associated with a different brand and variety (chicken broccoli and cheese). from 2005 to 2006, 27 salmonella enteritidis cases were associated with multiple varieties of product, pre ...200818939771
populations of salmonella enteritidis in artificially inoculated chicken eggs as influenced by the temperatures under which eggs might be held from the day of lay until the day of processing.this study was undertaken to determine the levels of salmonella enteritidis in artificially inoculated eggs as affected by the temperatures under which eggs might be held from the day of lay until the day of processing. unprocessed chicken eggs of different sizes (n=1920, with 480 being laid in each season) were inoculated in the albumen with a five-strain mixture of salmonella at 102 cfu per egg. the eggs were stored at 4, 10, and 22 degrees c for 3 weeks and sampled twice a week to determine t ...200818939755
membrane damage and viability loss of escherichia coli k-12 and salmonella enteritidis in liquid egg by thermal death time disk treatment.bacterial injury, including leakage of intracellular substance and viability loss, of escherichia coli k-12 (atcc 23716) and salmonella enteritidis (atcc 13076) inoculated in liquid egg white and liquid whole egg was determined by thermal death time disk. e. coli k-12 and salmonella enteritidis were inoculated in liquid egg white and liquid whole egg to a final count of 7.8 log cfu/ml and were thermally treated with thermal death time disks at room temperature (23"c), 54, 56, 58, and 60 degrees ...200818939742
a study of the distribution patterns and levels of salmonella enteritidis in the immune organs of ducklings after oral challenge by serovar-specific real-time pcr.the objective of this study was to understand the distribution patterns and levels of salmonella enteritidis (se) in the immune organs of ducklings after oral challenge. we conducted serovar-specific real-time polymerase chain reaction (pcr) for se to detect the genomic dna of se in the blood and immune organs, including the bursa of fabricius, thymus, spleen, and harderian gland, from ducklings after oral challenge at different time points. the results showed that se was consistently detected i ...200818939644
arabinoxylooligosaccharides from wheat bran inhibit salmonella colonization in broiler chickens.the objective of this in vivo experiment was to evaluate the influence of arabinoxylooligosaccharides (axos) on shedding and colonization of salmonella enteritidis in broilers. arabinoxylooligosaccharides, which are oligosaccharides derived from arabinoxylans by partial hydrolysis, have a beneficial effect on feed conversion ratios when added to broiler diets. additionally, axos have been shown to promote the growth of bifidobacteria in the cecocolonic compartment of the gastrointestinal tract. ...200818931184
direct enhancement of the phagocytic and bactericidal capability of abdominal macrophage of chicks by beta-1,3-1,6-glucan.salmonella enterica serovar enteritidis is the major zoonotic and intracellular pathogen. different strategies have been developed to prevent the s. enteritidis infection. the beta-1,3-1,6-glucan of schizophyllum commune was used as an immunological booster to determine the minimal dietary level of beta-glucan that would restrict s. enteritidis infection through the effects of beta-glucan on the activity of macrophages and direct physical protection of the intestine. one-day-old male single comb ...200818931174
the impact of temperature during the storage of table eggs on the viability of salmonella enterica serovars enteritidis and virchow in the eggs.salmonellosis is a foodborne infection of major economic importance. contamination of table eggs with salmonella, especially salmonella enterica serovar enteritidis, is a major health concern worldwide. recently, s. enterica serovar virchow has emerged as a major pathogen in israel, where it is among the 3 most prevalent serovars found in poultry and the second most prevalent serovar isolated from individuals with salmonellosis. although there is ample knowledge regarding the role of s. enterica ...200818931169
[hemolytic-uraemic syndrome. a review of 58 cases].our objectives were to determine epidemiology, clinical and laboratory characteristics of patients with haemolytic-uraemic syndrome (hus) treated in our centre, to describe renal and extra-renal complications and the treatment required and to relate our findings with the existing bibliography.200818928695
impact of reduced numbers of isolates phage-typed on the detection of salmonella outbreaks.the aim of this study was to assess the effects of reductions in the number of isolates tested by phage-typing on the recognition of outbreaks of salmonellosis. five outbreaks (categorized as 'small', 'medium' or 'large') which occurred in england in 2005 were used as examples. the outbreaks were caused by serotypes which were subdivided by phage-typing. results indicated that reducing the number of isolates phage-typed would have an impact on the surveillance system, with one outbreak likely to ...200918925987
antibiotic interrelationships among the enteric group of bacteria. 194718918670
activity of hexenolactone against salmonella enteritidis in mice. 194818865901
[not available]. 194818862804
atomic force microscopy investigation of phage infection of bacteria.atomic force microscopy (afm) was used to study the process of infection of bacterial cells by bacteriophages, for which purpose experimental protocols were elaborated. three types of bacteriophages were characterized with afm and transmission electron microscopy (tem). bacteriophage interaction with cells was studied for three bacterial hosts: gram-negative escherichia coli 057 and salmonella enteritidis 89 and gram-positive bacillus thuringiensis 393. depending on the phase of lytic cycle, dif ...200818850726
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