Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| immunology and genetics of zoonotic infections involving parasites. | zoonoses involving parasites are both common and important, some causing serious diseases. the abilities of such parasites to pass between humans and a variety of vertebrates facilitates their use as experimental model systems. much can be learned about the host-parasite relationships of zoonotic infections from laboratory-based studies, particularly where it is possible to make use of defined parasite isolates in a variety of host species or in genetically defined strains of a given host. this ... | 1996 | 8894375 |
| intestinal transport and catabolism of ige: a major blood-independent pathway of ige dissemination during a trichinella spiralis infection of rats. | previous work has shown that trichinella spiralis-infected rats transport ige from plasma to intestinal tissue and fluids. in this study we quantitate ige transport to the gut and circulation during t. spiralis infection in rats. total ige levels in intestinal fluid from infected rats were elevated by 4 days post-infection (dpi), but were not elevated in serum and lymph until 7 dpi. ige levels in intestinal fluid ranged from 1 to 6 microg between 10 and 21 dpi, and serum and lymph ige levels ran ... | 1996 | 8892637 |
| investigation of immunofluorescence cross-reactions against trichinella spiralis by western blot (immunoblot) analysis. | immunofluorescence cross-reactions in trichinella spiralis serodiagnosis are sometimes difficult to identify. we compared the results of an indirect immunofluorescence assay and the profiles obtained by western blot (immunoblot) analysis for three groups of patients: 10 t. spiralis-infected patients, 10 patients with autoimmune diseases, and 7 patients with parasitic diseases other than trichinellosis. the degree of immunofluorescence cross-reaction was variable. western blotting allowed us to d ... | 1996 | 8877138 |
| free and bound biotin molecules in helminths: a source of artifacts for avidin biotin-based immunoassays. | the avidin-biotin molecular recognition system is widely used in parasite immunology. however, the presence of biotin and/or biotin-containing molecules (bcms) in samples may lead to erroneous results. in the work reported herein we investigated the extent to which biotin and bcms present in helminth extracts may interfere in avidin/biotin-based immunoassays and developed an enzyme-linked immunosorbent assay (elisa) for quantification of these components. in avidin-based elisa using antinematode ... | 1996 | 8875569 |
| mast cells that reside at different locations in the jejunum of mice infected with trichinella spiralis exhibit sequential changes in their granule ultrastructure and chymase phenotype. | whether or not a nontransformed, mature mouse mast cell (mc) or its committed progenitor can change its granule protease phenotype during inflammatory responses, has not been determined. to address this issue, the granule morphology and protease content of the mc in the jejunum of balb/c mice exposed to trichinella spiralis were assessed during the course of the infection. within 1 wk after helminth infection of the mice, increased numbers of mc appeared in the crypts at the base of the villi, a ... | 1996 | 8858180 |
| susceptibility and reactivity of sheep to trichinella spiralis infection. | susceptibility and reactive manifestation to trichinella spiralis infection were studied in atypical hosts (sheep) for the period of 247 days. sheep produced anti-trichinella antibodies as early on day 11 (low titer 1:200), with maximum reached at day 35 (titer 1:800). from day 42 the antibody level was declining with a negative result of examination on day 70. mice exhibited anti-trichinella antibodies only on day 32 (titer 1:200). this level was rising, reaching high titer (1:1600) on day 56. ... | 1996 | 8856994 |
| expression and function of hla-dq8 (dqa1*0301/dqb1*0302) genes in transgenic mice. | transgenic mice expressing hla-dqa1*0301 and hla-dqb1*0302 genes (dq8) were produced. the transgenes were then transferred into mouse (ab degrees) class ii negative mice: the only class ii molecules expressed in these animals were therefore coded by the hla-dq8 genes. good expression of hla-dq molecules was found. both cd4+ t cells and dq8-specific t-cell receptor v beta expressing cells were positively selected in these mice. the hla-dq8 molecules expressed in these animals can present various ... | 1996 | 8834919 |
| histochemical investigations of the biochemical defence mechanism in experimental trichinellosis: ii. prostaglandin synthase activity. | in this paper, we report on the histochemical localization of prostaglandin synthase (e. c. 1.14.99.1) in mouse muscles following infection with trichinella spiralis and the dynamics of their changes at different stages of disease. our studies suggest that increased level of prostaglandin synthase activity in infected muscles of mice may be correlated with the degree of muscle injury by larvae and, perhaps, that increased level of enzyme ameliorates the functioning of muscle. the dynamics of pro ... | 1995 | 8826112 |
| histochemical investigations of the biochemical defence mechanism in experimental trichinellosis: i. peroxidase activity. | biological reactions caused by free radicals at the molecular and cellular levels engage many different biochemical components which can be directly damaged by the oxidizing radicals and which eventually promote pathological processes. defence mechanisms have evolved to limit the speed of production of free radical damage; they include low molecular antioxidants and antioxidant enzymes. in this study, the activity of peroxidase acting against peroxides appearing in muscle of mice at different st ... | 1995 | 8826111 |
| blocking anti-trichinella spiralis antibodies in chronically infected rats. | specific anti-newborn larva antibodies present in the serum of rats chronically infected with trichinella spiralis were incapable of inducing killing of newborn larvae by activation of normal peritoneal cells. these late antibodies blocked the cytotoxic reaction induced by early antibodies produced a few weeks after infection. passive transference of late serum to normal mice failed to induce protective immunity against infection by newborn t. spiralis larvae. when late immunoserum was fractiona ... | 1996 | 8825450 |
| injection of recombinant interleukin 3 hastens worm expulsion in mice infected with trichinella spiralis. | the effects of interleukin 3 (il-3) on worm expulsion were studied in mice infected with trichinella spiralis. c3h/he mice were treated with a total of 10(4) u il-3 or saline by daily peritoneal injection from day-5 to day-1. muscle larvae were given orally to both groups of mice on day 0. the muscle worn burden in infected mice was assessed on day 28. the worm burden in mice treated with recombinant il-3 (ril-3) was significantly suppressed as compared with that in control mice. a reduction in ... | 1996 | 8825203 |
| trichinella spiralis: ultrastructural localization of a target antigen recognized by a monoclonal antibody. | the localization of a 160-kda molecule recognized by a monoclonal antibody of the igg1-class (ts32d12) to trichinella spirilis was demonstrated at the subcellular level. mature muscle larvae recovered from infected mice were fixed with zamboni solution, and embedded in lr white resin. ultrathin sections were incubated with ts32d12 and subsequently stained using a secondary antibody-coated colloidal gold probe. ts32d12 reacted to constituents in the alpha-stichosome cells of the parasite; that is ... | 1996 | 8818736 |
| critical resources that influence habitat selection decisions by gastrointestinal helminth parasites. | habitat selection may be the basis of some of the most exciting questions in behavioural ecology today, but parasites are being excluded from this debate. parasites are not aberrant; they form a large proportion of the diversity of life on earth, and one estimate suggests that parasitism is more common than all other feeding strategies combined. we still do not understand the adaptive value of habitat selection behaviours in these organisms, even though the literature is full of examples of para ... | 1996 | 8818728 |
| stem cell factor dependent hyperplasia of mucosal-type mast cells but not eosinophils in schistosoma mansoni-infected rats. | stem cell factor (scf) is a growth factor with multiple activities which acts on numerous cell types including primordial germ cells, haemopoietic stem cells, melanocytes and mast cells. scf is critical for the development of the mast cell hyperplasia associated with infection with the intestinal parasites nippostrongylus brasiliensis and trichinella spiralis. in the present study we have assessed the role of scf in the mast cell and eosinophil responses to schistosoma mansoni in the rat by bloc ... | 1995 | 8817606 |
| immune cytotoxic activity of human eosinophils against trichinella spiralis newborn larvae. | the ability of human eosinophils to kill the newborn larvae (nbl) of trichinella spiralis of different maturation status, in the presence of antibody, was studied. a cytotoxic in vitro test was performed using nbl less than 2 h of age (nbl2) or nbl maintained in culture at 37 degrees c for 20 h (nbl20), peripheral blood eosinophils, anti-trichinella serum and human fresh serum as source of complement. under these experimental conditions eosinophils from normal individuals attached to nbl2 as wel ... | 1995 | 8817601 |
| trichinella pseudospiralis secretes a protein related to the trichinella spiralis 43-kda glycoprotein. | a 43-kda secreted glycoprotein from the intracellular parasitic nematode trichinella spiralis has been considered as a factor involved in the formation of the nurse cell in infected muscle. the closely related intracellular parasitic nematode trichinella pseudospiralis that also infects muscle cells, does not form nurse cells and was thought not to secrete the 43-kda glycoprotein. this implied a unique role for the 43-kda glycoprotein in t. spiralis infection and supported the hypothesis of invo ... | 1996 | 8813674 |
| developmental expression of a 43-kda secreted glycoprotein from trichinella spiralis. | trichinella spiralis is an intracellular parasitic nematode that infects skeletal muscle cells. infection results in loss of tissue specific characteristics and conversion of the muscle cell to a nurse cell. the characteristic changes leading to the formation of the nurse cell appear complete by day 12 after intramuscular infection. proteins synthesized in the stichocytes (secretory cells) of t. spiralis and secreted in the host cell are believed to be involved in the process of nurse cell forma ... | 1996 | 8813673 |
| trichinella spiralis infection in pigs in eastern bolivia. | trichinellosis in pigs in bolivia was first documented in 1993 following a small abattoir survey in a rural community in the bolivian altiplano. the present study investigated the presence of antibodies to trichinella spiralis in pigs in the 2 largest departments in terms of pig production in bolivia. three geographically separate abattoir surveys were conducted to cover the major production areas in the departments of santa cruz and chuquisaca. sera were tested using an enzyme-linked immunosorb ... | 1996 | 8809974 |
| uvb-induced decreased resistance to trichinella spiralis in the rat is related to impaired cellular immunity. | our laboratory has demonstrated in preliminary experiments that uvb exposure using the kromayer lamp can induce increased numbers of trichinella spiralis larvae in carcasses of infected wistar rats, without affecting specific antibody titers to this parasite. in this study, orally t. spiralis-infected wistar rats were exposed to suberythemal doses of uvb radiation using fs40 lamps during different time periods before or after infection. a significant increase in the number of t. spiralis larvae ... | 1996 | 8806234 |
| effects of oral l-name during trichinella spiralis infection in rats. | we investigated the involvement of nitric oxide in transmural jejunal alterations induced by trichinella spiralis (t. spiralis) infection in rats. rats were gavaged with either saline or t.spiralis larvae, and, 1 h later, rats were treated orally with water, ng-nitro-l-arginine methyl ester (l-name; 30 mg/kg), or ng-nitro-d-arginine methyl ester (d-name; 30 mg/kg) on a daily basis. although not observed in jejunum from uninfected rats, inducible nitric oxide synthase (inos) mrna was present in t ... | 1996 | 8770050 |
| anthelmintic activity of 6,7-diaryl-pteridines. | in a search for new anthelmintic compounds, some 6,7-diaryl-pteridines were synthesized from the corresponding diaminopyrimidines and aromatic aldehydes. their anthelmintic activity was tested in vitro against caenorhabditis elegans and heligmosomoides polygyrus and in vivo against trichinella spiralis. structure-activity relationships are discussed. | 1996 | 8767359 |
| [the heidelberg trichina dispute. a parasitologic episode from the year 1840]. | since the initial zoological description of "trichina spiralis" (later renamed "trichinella spiralis") by owen in 1835 several other scientists had observed trichinas in human muscles, when an inglorious dispute about priorities arose between the anatomists kobelt and bischoff at the heidelberg university in 1840 because of one of those findings. the genesis of trichinosis was only to be explained in 1860 by zenker, while bischoff, who was among the leading 19th century embryologists, then still ... | 1996 | 8765543 |
| neural integration of jejunal motility and ion transport in nematode-infected ferrets. | jejunal motility measured manometrically and coupled fluctuations in electrogenic ion transport measured as transmural potential differences (pd) were simultaneously studied in ferrets infected with enteric stages of the parasitic nematode, trichinella spiralis. vagotomy in uninfected ferrets abolished jejunal motility clusters and associated pd oscillations. conversely, in infected ferrets on days 8-12 postinfection (pi), vagotomy did not abolish jejunal motility and pd. calculated motility ind ... | 1996 | 8760106 |
| [phosphocholine antigens in muscle larvae of trichinella spiralis]. | crude t. spiralis larval antigens often cross-react with antibodies to other parasites (for instance toxocara canis) in the elisa test. the crossreactivity of the sera is probably due to the presence of phosphocholine (pc)-specific antibodies to antigens found in the inner layer of the cuticle, in the haemolymph, genital primordium and glycogen aggregates t. spiralis larvae. the anti-pc response following infection with t. spiralis occurs earlier than the antispecific antigens response. the abov ... | 1996 | 8755146 |
| propionibacterium granulosum activities in rats during the muscle phase of trichinella spiralis invasion. | immune response of wistar rats, infected with 4000 l1 t. spiralis and treated with p. granulosum during the muscle phase of nematode invasion were measured. the increase of spleen mass was observed in all groups infected and exposed to p. granulosum. intraperitoneal injection of bacteria results in higher level of t lymphocytes and activated neutrophils. the level of inhibition of macrophages migration was depended on relation to the time and doses of injection. in non-specific stimulated animal ... | 1996 | 8755145 |
| effect of some parasitic infection on neurotransmitters in the brain of experimentally infected mice before and after treatment. | the effects of some parasitic infection (bilharziasis, toxocariasis and trichinosis) on the brain of experimentally infected mice were investigated. eighty animals were classified into four groups, group i contained five non infected animals as a control group. the other groups each contained twenty-five mice infected with schistosoma mansoni (group ii), toxocara canis (group iii) and trichinella spiralis (group iv). each infected group was divided into two subgroups (a,b). subgroup (a) left unt ... | 1996 | 8754657 |
| detection of gastrointestinal nematodes by a coproantigen capture elisa. | an assay is described for the quantitative detection of excretory/secretory antigens liberated by the intestinal nematode heligmosomoides polygyrus and voided in the host's faeces. a rabbit polyclonal antiserum to the antigens detected them in a dose-dependent manner and was sufficiently sensitive to recognise infection in mice carrying a mean worm burden of nine worms. the assay was specific, giving higher optical density readings with the faeces of mice infected with h polygyrus than with faec ... | 1996 | 8745247 |
| antigenic cross-reactivity in mice between third-stage larvae of anisakis simplex and other nematodes. | we used elisa and immunoblotting to investigate antigenic cross-reactivity in mice between third-stage larvae of anisakis simplex and five other nematodes: the ascaridoids ascaris suum, toxocara canis and hysterothylacium aduncum, and the nonascaridoids trichinella spiralis and trichuris muris. two sera were raised against each species (including a. simplex, but excluding a. suum), by infection or by immunization with somatic antigens. serum against a. suum was raised by immunization only. the r ... | 1996 | 8740558 |
| generation of tnf alpha, ifn gamma, il-6, il-4 and il-10 in mouse serum from trichinellosis: effect of the anti-inflammatory compound 4-deoxypyridoxine (4-dpd). | infections caused by the nematode trichinella spiralis is characterized in the host by an inflammatory response with cytokine production. in these studies we have detected tnf alpha, il-6, ifn gamma, il-4 and il-10 in the serum of 10 mice infected with t. spiralis. moreover, we detected, for the first time, these cytokines in the serum of mice treated with 4-dpd, a potent antagonist of vitamin b6 coenzyme which has anti-inflammatory properties. 4-dpd was used at 100, 400, 800 micrograms/bolus fo ... | 1996 | 8739314 |
| effects of maternal ethanol consumption on the subsequent development of immunity to trichinella spiralis in rat neonates. | the immune response of rat pups to the intestinal parasite trichinella spiralis was studied to determine if maternal pre- and/or postnatal ethanol consumption affected neonatal immune responses. female rats were fed ethanol-containing (36% of calories) or pair-fed control liquid diets and include groups that were maintained on ethanol as follows: group 1, from day 1 of pregnancy through weaning and whose pups were then placed on ethanol to sacrifice; group 2, from day 1 of pregnancy through lact ... | 1996 | 8727247 |
| immunization against trichinella spiralis using antigens from different life-cycle stages experimental study in mice. | crude antigen preparations from different life-cycle stages of t. spiralis using 10 micrograms and 100 micrograms antigen/dose, were tested for their ability to immunize mice against homologous challenge infection. immunization was assessed by accelerated expulsion of adult worms, reduction in female worm fecundity and decreased yield of muscle larvae. the dose of 100 micrograms proved to be more protective in all antigen preparations. the aa provided highly significant protection against adult ... | 1996 | 8721225 |
| trichinella spiralis and trichinella pseudospiralis induce collagen synthesis by host fibroblasts in vitro and in vivo. | immunoperoxidase staining of muscle infected with trichinella spiralis for murine collagen types i and iv provided both qualitative and quantitative evidence of extensive synthesis of both types of collagen by fibroblasts in infected muscle compared to that seen in uninfected muscle. moreover, fibroblasts in muscle infected with t. pseudospiralis, a nonencapsulating species, showed significantly less staining for both types of collagen compared to muscle from mice infected with t. spiralis. anal ... | 1995 | 8719950 |
| the influence of the invasion of trichinella sp. on immunologic reactions in mice: 1. analysis of the reaction of skin allograft rejection. | 1996 | 8689523 | |
| lung eosinophilia in response to intravenously injected trichinella spiralis. | mice were injected intravenously with killed muscle-stage larvae of the parasitic nematode trichinella spiralis. this induced eosinophilic lung inflammation, the degree of eosinophilia was detected and measured by broncho-alveolar lavage. subsequent in vivo restimulation by trichinella antigens produced an enhanced lung response, showing that exposure to killed larvae generated an effective immunological memory. the kinetics and characteristics of the eosinophil response suggested that it was lo ... | 1996 | 8688865 |
| acceleration of ige responses by treatment with recombinant interleukin-3 prior to infection with trichinella spiralis in mice. | treatment of mice with recombinant interleukin-3 (ril-3) accelerated an il-4-dependent ige production following infection with trichinella spiralis. when mice were treated with a total of 1.5 x 10(4) units ril-3 for 5 days before infection with 400 muscle larvae, the serum ige level increased prominently on day 5. acceleration of ige responses was dependent on the dose of ril-3 injected. treatment of mice with a total of 10(3) units ril-3 could accelerate ige responses. ige responses were detect ... | 1996 | 8675221 |
| a critical role for stem cell factor and c-kit in host protective immunity to an intestinal helminth. | in common with many intestinal nematode infections, trichinella spiralis infections in mice are associated with a pronounced intestinal mast cell hyperplasia. the expulsion of the parasite from the gut is temporally associated with intestinal mastocytosis and mast cell function reflected by the secretion of mast cell protease into tissue and serum. in vivo, mucosal mast cell production is highly dependent upon t cell-derived cytokines including il-3 and il-4. we present data here to show that in ... | 1996 | 8671643 |
| local intestinal immune responses to infections with trichinella spiralis. real-time, continuous assay of cytokines in the intestinal (afferent) and efferent thoracic duct lymph of rats. | levels of il-4, il-5, tnf-alpha, and ifn-gamma were quantitated in the intestinal (afferent) and efferent thoracic duct lymph of rats during the course (0 to 289 h) of an infection with trichinella spiralis. intestinal lymph was collected by cannulating thoracic ducts of mesenteric lymphadenectomized animals. these studies showed that cytokines typical of a th2 type (il-4 and il-5) and a th1 type (ifn-gamma) were simultaneously detected in the intestinal lymph during the first 8 days after infec ... | 1996 | 8666804 |
| clinical aspects of infection with trichinella spp. | isolated cases and outbreaks of infection with trichinella spp. occur frequently throughout the world, sometimes resulting in fatalities. the clinical presentations of signs and symptoms are remarkably constant for most of the species of trichinella, but in infections with trichinella nativa and trichinella britovi, classical symptoms of trichinellosis may be absent. it is important to be able to correlate the clinical presentation of trichinellosis with the life cycle of these helminths in orde ... | 1996 | 8665476 |
| structural organisation and lipid composition of the epicuticular accessory layer of infective larvae of trichinella spiralis. | the epicuticle of infective larvae of trichinella spiralis represents the interface between this intracellular nematode parasite and the cytosol of mammalian skeletal muscle. the macromolecular structures that make up the epicuticle were studied by freeze-fracture electron microscopy and compositional analysis. three fracture planes were observed: one with a typical plasma membrane-type bilayer organisation which was overlaid by two extended layers of lipid in an inverted cylindrical configurati ... | 1996 | 8652610 |
| nutritional support for adaptation to radiation-induced suppression of mucosal immunity in the intestine of the rat. | appropriate enteral nutrition provided immediately after injury or trauma to the gastrointestinal tract may limit or reverse damage to the mucosal barrier. in this regard, diets containing amino acids, such as arginine and glutamine, or fish oil have been identified as beneficial. this report assesses the role of amino acids as "essential nutrients" in the repair of intestinal mucosa damaged by gamma radiation. rats were used experimentally to test the hypothesis that the recovery of the immune ... | 1996 | 8643836 |
| experimental trichinellosis in goats. | the susceptibility and distribution of trichinella spiralis infection in goats were examined in ten autochthonous kids, 2 months old and about 10 kg body weight. the animals were divided into two groups: one experimental group with eight animals, infected with 10,000 t. spiralis 't1' encysted larvae and a control group with two non-infected animals. all the animals of the experimental group infected by the parasite showed that trichinella larvae have a special affinity for the tongue, masseters, ... | 1996 | 8638385 |
| [the efficiency of the serological reactions with trichinella spiralis somatic antigen in the diagnosis of human trichinelliasis caused by infection with natural and synanthropic trichinella isolates]. | the efficiency of serological tests (enzyme immuno-assay (eia) and indirect hemagglutination test (ihat)) using the antigen t.spiralis was examined on 318 sera from trichinosis patients infected via pork meat and on 112 sera from those infected via wild animal meat. this was estimated at 85.6 and 84.8% with eia and 93.0 and 85.7% with ihat in the two groups, respectively. to enhance the efficiency of serological diagnosis of trichinosis, it is recommended that eia and ihat should be used concurr ... | 1995 | 8596508 |
| heat shock response of trichinella spiralis and t. pseudospiralis. | heat shock proteins (hsps) were documented for the first time in both somatic extracts and excretory/secretory (es) products of the infective-stage larvae of trichinella spiralis and t. pseudospiralis. larvae recovered from muscles of infected mice were heat shocked at 37, 40, 43 and 45 degrees c in rpmi 1640 medium containing l(-)[35s]methionine. somatic extracts and es products of heat-shocked worms were then analysed by sds-page, autoradiography and laser densitometry. prominent bands of hsps ... | 1996 | 8587806 |
| four outbreaks of human trichinellosis in henan province. | four outbreaks of human trichinellosis caused by eating pork together had occurred successively in two rural areas and two cities of henan province, where the disease is endemic. of the 110 persons involved, 54 had the onset. all of them had the history of eating pork. in the outbreak of dengzhou, in those persons who dined together, men were all uninfected and 13 women were infected. the difference found between sexes was suggested to be related with spirit drinking. in these 54 patients, the l ... | 1995 | 8585984 |
| restriction fragment length polymorphism (rflp) analysis of genomic dna of 5 strains of trichinella spiralis in china. | five restriction endonucleases were used to digest genomic dna from 5 isolates of trichinella spiralis obtained from changchun, tianjin, xian, henan and yunnan. all the isolates were secured from pigs except the changchun strain which came from dog. the dna fragments digested by endonuclease were separated by agarose gel electrophoresis. the changchun isolate had a ecori band at 1.12kb and a drai band at 1.97kb which were unique to this isolate. a cloned specific repetitive dna sequence (1.12kb) ... | 1995 | 8580480 |
| molecular analysis of antigens targeted by protective antibodies in rapid expulsion of trichinella spiralis [mol. biochem. parasitol. 65 (1994) 201]. | 1995 | 8577344 | |
| the mycobacterial component of complete freund's adjuvant induces expulsion of the intestinal nematode trichinella spiralis in mice. | expulsion of t. spiralis adult worms was accelerated in high-responder nih mice vaccinated previously with complete freund's adjuvant (cfa) emulsified in pbs, whereas the expulsion of this parasite from low-responder c57 bl/10 mice was unaffected. incomplete freund's adjuvant (ifa) did not induce protection and it was concluded that the mycobacterial component, present in cfa but absent from ifa, was responsible for inducing these non-specific protective effects. cfa did not induce t. spiralis-s ... | 1996 | 8574244 |
| eu experimental study on wild boar trichinellosis. | from january 1994 onwards the council directive 92/45 eec concerning the examination of wild game meat for trichinellosis is valid. laboratory methods required are identical to those used for the examination of pork. in an international experiment the suitability of these methods to control wild boar meat was tested. required meat parts of experimentally with t. spiralis infected wild boars were shipped to seven laboratories in europe under code. it was concluded that trichinoscopy and pool samp ... | 1996 | 8574243 |
| ultrastructural colocalization of phosphorylcholine and a phosphorylcholine-associated epitope in first-stage larvae of trichinella spiralis. | although the presence of phosphorylcholine (pc) in trichinella is well established, the structures of the tsl-4 antigens that bear this epitope are unknown. a subset of tsl-4 antigens (tsl-8 antigens) has been reported to be absent from the surface of first-stage t. spiralis larvae. we report experiments with a monoclonal antibody (mab us2) developed in mice with a relative inability to produce antibodies to pc. in immunoblotting, mab us2 and anti-pc mab (bh8) showed apparently identical binding ... | 1995 | 8570578 |
| high levels of protection induced by a 40-mer synthetic peptide vaccine against the intestinal nematode parasite trichinella spiralis. | parenteral vaccination, using a 40-mer synthetic peptide from a 43,000 mw immunodominant glycoprotein secreted by the intestinal nematode parasite trichinella spiralis, induced high levels of protection against a subsequent challenge infection in mice. the expulsion of adult worms from the gut was accelerated following vaccination with peptide 40-80, but peptides 81-120 and 121-160 were not protective. mesenteric lymph node cells taken from t. spiralis-infected mice showed some proliferation in ... | 1995 | 8567011 |
| concurrent infection with sibling trichinella species in a natural host. | random amplified polymorphic dna (rapd) analysis of individual trichinella muscle larvae, collected from several sylvatic and domestic animals in estonia, revealed concurrent infection of a racoon dog with trichinella nativa and trichinella britovi. this finding provides strong support for their taxonomic ranking as sibling species. these 2 species appear uniformly distributed among sylvatic animals through estonia, while trichinella spiralis appears restricted to the domestic habitat. | 1995 | 8557472 |
| [characteristics and recent trends in endemicity of human parasitic diseases in china]. | an analysis of the nationwide survey of the distribution of human parasites in the light of the literature previously published, two major trends in the endemicity of parasitic diseases in china was observed. for one trend, the infection rate of e. histolytica, fasciolopsis buski and soil-transmitted helminthasis etc. declined markedly. the second trend was that the prevalence of food-transmitted parasitic diseases (trichinella spiralis, clonorchis sinensis, paragonimiasis, cysticercosis) and hy ... | 1995 | 8556800 |
| oral and parenteral vaccination against trichinella spiralis infections in high- and low-responder mice. | vaccination by different routes and with different adjuvants is known to influence profiles of immune responses and may be used to overcome genetically determined low-responsiveness to infection. a mouse model of infection with the intestinal nematode trichinella spiralis was used to investigate the effect of mode of vaccination upon immune responsiveness and worm expulsion phenotype in high- (nih) and low- (c57 bl/10) responder strains of mice. muscle larval homogenate antigen was given subcuta ... | 1995 | 8550299 |
| molecular cloning and expression of an immunodominant 53-kda excretory-secretory antigen from trichinella spiralis muscle larvae. | 1995 | 8538697 | |
| a role for second messengers in the control of activation-associated modification of the surface of trichinella spiralis infective larvae. | the involvement of second messengers in the control of activation-induced changes to the surface of trichinella spiralis infective larvae was investigated using membrane-permeant photo-activatable 'caged' compounds to alter intracellular levels of inositol trisphosphate (ip3), calcium ions (ca2+) and cyclic amp (camp). activation of larvae by incubation in culture medium containing trypsin and bile was followed by the loss of the surface coat labelled with the fluorescent pkh26 lipid probe and t ... | 1995 | 8538685 |
| detection of circulating trichinella spiralis muscle larva antigens in serum samples of experimentally and naturally infected swine. | a follow up study was carried out to determine the kinetics of appearance of surface/stichosomal (s/s) components, recently included in the tsl-1 group of trichinella spiralis muscle larva (ml), in serum samples from 13 experimentally infected pigs. detection of circulating antigens in these animals was done by a sandwich enzyme-linked immunosorbent assay (elisa) using t. spiralis specific rabbit polyclonal immunoglobulins to capture free antigens and monoclonal antibody nim-m1 to recognize s/s ... | 1995 | 8533271 |
| anti-nematode activity of sixteen compounds against trichinella spiralis in mice--a possible new screen for macrofilaricides. | there are few small animals models for filariasis, even more so for onchocerciasis. therefore it is difficult to test under drug screening conditions large numbers of potentially macrofilaricidal compounds. one way around this difficulty is to use mice infected with trichinella spiralis which by reason of anatomical location in the host would show some correlation in antinematode activity between the test and target organisms. this study investigated the activity of 16 compounds against the imma ... | 1995 | 8525399 |
| ultrastructural study of trichinella spiralis with emphasis on adult female reproductive organs. | the ultrastructure of the reproductive organs of adult female t. spiralis was described on the basis of extensive observation under an electron microscope. the organisms were recovered from the host intestine 7 days after oral infection. the organs consisted of a single ovary, seminal receptacle, uterus and vagina, confirming the reports described by previous authors at light microscopical level. all the organs were surrounded by a basal lamina and epithelial cells, and were bathed in haemolymph ... | 1995 | 8522769 |
| the mucosal and systemic response to phosphorylcholine in mice infected with trichinella spiralis. | the presence of phosphorylcholine (pc) in trichinella was confirmed by elisa and western blot experiments with the pc-specific myeloma tepc-15. anti-pc antibody production was detected in elisa by cross-reaction with the pc-positive somatic polysaccharide of aspergillus and the synthetic conjugate phosphorylcholine-bovine serum albumin conjugate and by inhibition with phosphorylcholine chloride (pccl). the kinetics of the serum and mucosal anti-pc immunoglobulin response were determined followin ... | 1993 | 8513878 |
| trichinella spiralis: the effect of specific antibody on muscle larvae in the small intestines of weaned rats. | weaned rats were passively immunized with rat igg2c monoclonal antibodies previously shown to protect infant rats against challenge with trichinella spiralis. although the antibodies did not protect rats against infection, the course of larval establishment in the intestine was altered in a way that has not been described previously. specifically, larvae that invaded the epithelium were inhibited from migrating from intestinal tissue into saline during the standard recovery procedure. the inhibi ... | 1993 | 8513877 |
| trichinosis with ventilatory failure and persistent myocarditis. | life-threatening infections with trichinella spiralis are rare in countries that have adopted laws requiring cooking of raw garbage fed to pigs. thus such infections may pose a diagnostic dilemma for clinicians unfamiliar with their presentation. we report a case of imported trichinosis in a mexican national who developed respiratory failure, myocarditis, and sinus arrest. the patient recovered uneventfully after the administration of benzimidazole and corticosteroid drugs, although a pacemaker ... | 1993 | 8513055 |
| a morphological study of the effects of liposomized albendazole on the muscle phase of trichinella spiralis in mice. | the pathomorphological effects of daily and weekly doses of liposomized albendazole on trichinella spiralis larvae have been examined and compared during the course of muscle infections in mice. treatment with three and six daily doses of the drug given by intraperitoneal injection to mice resulted in marked pathological alterations to encapsulated larvae, mainly in the walls of the capsules. there was a 5% efficacy against t. spiralis in mice given three daily doses of albendazole, and 38% effi ... | 1993 | 8509615 |
| arbitrarily primed polymerase chain reaction of individual trichinella specimens. | recently, 5 sibling species and 3 other phenotypes were identified in the genus trichinella. single primers of arbitrary nucleotide sequence were used to produce random amplified polymorphic dna starting from decreasing amounts of trichinella spiralis and trichinella britovi dna. reproducible amplification products from 30 pg of dna were obtained using 1 of 6 examined primers. these fragments distinguish between 2 european trichinella species, t. spiralis, showing a 1,350-bp band, and t. britovi ... | 1993 | 8501603 |
| biochemical, biological, and genetic characterization of a sylvatic isolate of trichinella. | biological, biochemical, and genetic relationships of trichinella isolates were assessed and compared from 3 species of illinois fur-bearing mammals. tongue muscle collected from 1987 through 1989 from 323 raccoons (procyon lotor), 9 red fox (vulpes fulva), and 1 coyote (canis latrans) were digested and trichinella muscle larval prevalences and mean intensities (larvae/g) determined. the prevalence and mean intensity of tongue muscle-stage larvae were 2.8% and 44.4% and 326 and 2 larvae/g for ra ... | 1993 | 8501590 |
| human recombinant interleukin 1 beta suppresses acetylcholine release from rat myenteric plexus. | a marked suppression of acetylcholine (ach) release from myenteric nerves in the inflamed intestine of rats infected by trichinella spiralis has been shown. in this model, there is increased expression of interleukin 1 beta (il-1 beta) in the myenteric plexus. therefore, the ability of il-1 beta to alter ach release in longitudinal muscle-myenteric plexus (lmmp) preparations from noninfected rats was examined. | 1993 | 8500722 |
| trichinella spiralis infected skeletal muscle cells arrest in g2/m and cease muscle gene expression. | infection by trichinella spiralis causes a variety of changes in skeletal muscle cells including the hypertrophy of nuclei and decreased expression of muscle specific proteins. potential cellular processes leading to these changes were investigated. in synchronized muscle infections, [3h]thymidine was incorporated into infected cell nuclei from 2-5 days post infection. labeled nuclei were stably integrated into the infected cell up to 60 days post infection and appear to originate from different ... | 1993 | 8491772 |
| studies on tissue cholinesterase in experimental trichinosis in albino rat. 1. effect of t. spiralis infection. | adult male albino rats were orally infected with trichinella spiralis (t. spiralis) larvae (400 larvae/rat). the cholinesterase (che) activity was then determined in the serum, brain, spinal cord, liver, stomach, intestine, heart, diaphragm and gastrocnemius muscle of the infected rats at different time intervals (15, 30, 45, 60 and 90 days) after infection. the enzyme activity was inhibited at all the time intervals in the brain, liver, heart and diaphragm while it increased progressively in th ... | 1993 | 8482886 |
| studies on tissue cholinesterase in experimental trichinosis in albino rat. 2 effects of treatment with mintezol and 5-fluorouracil-endoxan. | the changes in cholinesterase (che) activity was studied in different tissues of normal and 60 days t. spiralis infected albino rats following treatment with mintezol (5 mg/rat/day, for 5 days) and 5-fluorouracil-endoxan (500 mg/rat/day--100 mg/rat/day, for 5 days). in normal rats, administration of either mintezol or 5-fluorouracil-endoxan provoked a general decrease in the che activity of the various rat tissues. treatment of 60 days t. spiralis infected rats with mintezol increased markedly t ... | 1993 | 8482867 |
| studies on t. spiralis and t pseudospiralis larvae recovered from mice immunized with heterologous trichinella antigen. | in this study, s3 antigen of t. spiralis and t. pseudospiralis was given to the heterologous infection in albino mice. the recovered larvae were studied as regards: their number, length and steroscan examination. it was found that vaccination of mice with t. spiralis (s3) antigen induced reduction in t. pseudospiralis larval count by 66.43% and significant reduction in length. using t. pseudospiralis (s3) antigen reduction of 74.77% in t. spiralis larval count was detected as well as significant ... | 1993 | 8482862 |
| comparison of human trichinellosis caused by trichinella spiralis and by trichinella britovi. | the first documented report of an outbreak of human trichinellosis caused by trichinella spiralis in italy is described. two family groups were involved. the source was wild boar meat products. the principal clinical features were fever (60%), myalgia (50%), and diarrhea (40%). the most useful laboratory indicators were eosinophilia (100%), elevated levels of creatine phosphokinase (cpk) (90%) and other muscle enzymes, parasite-specific igg titers (100%), and anti-newborn larvae antibodies (30%) ... | 1993 | 8480866 |
| downregulation of neutral endopeptidase (ec 3.4.24.11) in the inflamed rat intestine. | intestinal inflammation induced by the nematode trichinella spiralis is accompanied by increased intestinal concentrations of substance p, a mediator of inflammation and a stimulant of smooth muscle contraction, and by intestinal hypermotility. the expression of neutral endopeptidase (nep), a cell surface enzyme that degrades substance p in the extracellular fluid, was examined in the inflamed intestine. nep enzymatic activity, measured by a fluorometric assay, was reduced by 84-fold in jejunal ... | 1993 | 8476057 |
| trichinella spiralis--characterization of circulating and immune-complex-associated antigens. | 1993 | 8469676 | |
| requirements for the induction of cross-reactive anti-trichinella ige antibodies in mice. | mice primed with trichinella spiralis or t. pseudospiralis and reinfected with either the homologous or the heterologous species produced high levels of ige antibodies that cross-reacted with the non-inducing strain in passive cutaneous anaphylaxis assays. cross-reactive antibodies were not induced by primary infection. cross-reactivity persisted for more than 6 months following secondary infection or destruction of encysted larvae with mebendazole. both the prevention of larvi-position by thiab ... | 1993 | 8469671 |
| on the specificity of altered muscle function in experimental colitis in rats. | studies on muscle contraction in colitis yield conflicting data that may reflect differences in the manner in which colitis is induced. therefore, we compared distal colonic longitudinal muscle contraction in four models of colitis in the rat. | 1993 | 8462793 |
| detection of muscle larvae of trichinella spiralis by enzyme-linked immunosorbent assay. | elisa was applied to the detection of trichinella spiralis larvae in infected rat muscles. anti-muscle larva serum was prepared by inoculating crude extract of muscle larvae to rabbits. limb muscles of infected rats were emulsified with carbonate buffer and the supernatant was used for elisa. elisa values of positive muscular samples were clearly higher than those of negative ones to prove that elisa can be applied to the detection of muscle larvae. the present method showed positive values to m ... | 1993 | 8461404 |
| anthelmintic activity of pyrazinothiadiazine dioxide derivatives. | in a search for new anthelmintic compounds, some pyrazinothiadiazine dioxide derivatives were synthesized. their anthelmintic activity was tested against larva and preadult stages of trichinella spiralis. the mode of action and acute toxicity of these compounds were investigated. structure-activity relationships are discussed. | 1993 | 8457240 |
| trichinella spiralis: the effect of oral immunization and the adjuvancy of cholera toxin on the mucosal and systemic immune response of mice. | the effect of oral immunization and the adjuvancy of cholera toxin (ct) were examined in mice infected with trichinella spiralis. the mean of total muscle larvae was reduced by 36% in mice infected with trichinella larvae in combination with ct. in mice fed soluble, particulate, or soluble/particulate antigens in combination with ct on days 0, 14, and 21, and challenged with trichinella larvae on day 28, there was a significant reduction in adult worm fecundity (50%), worm size (20-30%), and the ... | 1993 | 8454027 |
| diarrhea in helminthic infections. | enteric helminths are among the most prevalent parasites of humankind, yet only scanty information exists about their effects on the gastrointestinal tract of their hosts. specifically, there is little agreement on which worms definitely cause diarrhea. the available evidence suggests that five helminthic parasites are associated with human diarrheal disease: trichinella spiralis (early phases of infection), trichuris trichiura, strongyloides stercoralis, capillaria philippinensis, and schistoso ... | 1993 | 8452963 |
| a new cell culture method (the lateral diffusion system) suitable for the induction of antibody-forming cells in vitro. | the lateral diffusion system (lds) is a new cell culture method suitable for the in vitro induction of antibody-forming cells, based upon the diffusion of nutrients and other molecules between two contiguous compartments separated by a vertical microporous membrane. we compared an lds cell culture plate with two commercially available diffusion culture units, the marbrook chamber (c.a. hendley ltd.) and the millicell-ha cell culture unit (millipore corporation), in terms of ability to support sp ... | 1993 | 8445243 |
| immunotoxic effects of the color additive caramel color iii: immune function studies in rats. | administration of the color additive caramel color iii (ac) may cause a reduction in total white blood cell counts in rats due to reduced lymphocyte counts. beside lymphopenia, several other effects in rat have been described. the effects are caused by the imidazole derivative 2-acetyl-4(5)-(1,2,3,4-tetrahydroxybutyl)imidazole (thi) and occur in rats fed a diet low in vitamin b6. in the present paper, immune function studies on ac and thi with rats fed a diet low, but not deficient in vitamin b6 ... | 1993 | 8432426 |
| differentiation of trichinella isolates by polymerase chain reaction. | oligonucleotide primers were synthesized for the polymerase-chain-reaction amplification of target dna from two sequences of trichinella spiralis. six strains belonging to t. spiralis, t. nativa, t. britovi, t. pseudospiralis, and t. nelsoni were tested. amplification products were obtained with t. spiralis, t. britovi, and t. nelsoni dna from a 53-kda antigen cdna sequence and with t. spiralis and t. nelsoni dna from a 1.6-kb repetitive dna sequence. differences in the length of the amplificati ... | 1993 | 8415558 |
| immunological relationships during primary infection with heligmosomoides polygyrus (nematospiroides dubius): downregulation of specific cytokine secretion (il-9 and il-10) correlates with poor mastocytosis and chronic survival of adult worms. | mice were infected either with trichinella spiralis (day 0), heligmosomoides polygyrus (day-14) or concurrently with both species and were killed in groups, together with naïve control mice, on 2 occasions (day 8 and 15 post infection with t. spiralis, corresponding to days 22 and 29 p.i. with h. polygyrus). the expulsion of t. spiralis was slowed significantly in concurrently infected mice and this was associated with a reduced mastocytosis and lower serum mucosal mast cell protease levels. mes ... | 1993 | 8414644 |
| expression of a protective intestinal immune response can be inhibited at three distinct sites by treatment with anti-alpha 4 integrin. | the alpha 4 integrins mediate lymphocyte adhesion to peyer's patch high endothelial vessels and homing to peyer's patch, as well as to mesenteric lymph nodes. in rats, immunity to infection with the nematode trichinella spiralis is known to be mediated by cd4+ ox22- (cd45rc-) cells that home to the intestine in large numbers. these experiments were conducted to determine whether the alpha 4 integrins or lfa-1 were involved in the expression of intestinal immunity to t. spiralis. injection of the ... | 1993 | 8409437 |
| the protective role of fasciola tegument antigen against trichinella spiralis infection. | fasciola gigantica tegument antigen was prepared to be used for induction of immunity against trichinella spiralis in albino mice. the results of the study showed that the antigen was more effective when administered in a dose of 3 micrograms than in a dose of 30 micrograms. this was evidenced by the reduction in adult count 72%, adult length 61.4%, larval count 78.7%, length of muscle larvae 32.2% and the significant reduction in reproductive capacity index. | 1993 | 8376868 |
| reversible expression of mouse mast cell protease 2 mrna and protein in cultured mast cells exposed to il-10. | balb/cj mouse mast cells derived by culturing bone marrow progenitor cells in wehi-3 cell-conditioned medium (bmmcw) do not contain mouse mast cell protease 2 (mmcp-2) mrna, but these cells can be induced to express this transcript after exposure to ril-10. to study the translation and granule accumulation of mmcp-2 in ril-10-treated bmmc (bmmcw+il-10), a rabbit antibody was developed to a synthetic peptide that corresponds to the novel amino acid sequence in mmcp-2 at residues 56 to 71. after a ... | 1993 | 8376776 |
| biochemical and immunological characterization of multiple glycoforms of mouse mast cell protease 1: comparison with an isolated murine serosal mast cell protease (mmcp-4). | five highly soluble, chymotrypsin-like, neutral serine proteases, with molecular masses in the range 30-33 kda, were isolated from trichinella spiralis-infected mouse small intestine. these enzymes were closely related antigenically on western blotting and by ouchterlony double diffusion using a polyclonal, cross-absorbed, sheep antibody raised against mouse mast cell protease-1 (mmcp-1) and on the basis of n-terminal amino acid sequence analysis, were identified as variant forms of mmcp-1. subs ... | 1993 | 8363563 |
| effects of small doses of dioxins on the immune system of marmosets and rats. | there is no doubt that tcdd is capable of inducing effects on a variety of components and functions of the immune system in a variety of species. in fact, such changes seem to belong to the most sensitive variables affected by tcdd. some of the biological effects, induced at rather high doses of tcdd exhibiting general toxicity (> 3 micrograms tcdd/kg body wt), may be considered unspecific or the result of the pronounced thymus involution. however, other effects (such as that on lymphocyte subty ... | 1993 | 8363273 |
| rapid changes in the surface of parasitic nematodes during transition from pre- to post-parasitic forms. | all mammalian-parasitic stages of a range of nematode species investigated (brugia pahangi, acanthocheilonema viteae, strongyloides ratti, nippostrongylus brasiliensis, trichinella spiralis and ostertagia ostertagi) labelled in a surface-restricted manner with the fluorescent lipid analogues 5-n-(octadecanoyl)aminofluorescein (af18) or nitrobenzoxadiazole-cholesterol (nbd-chol), but failed to bind other similar probes. in contrast, the surfaces of the 'pre-parasitic' infective stages of these sp ... | 1993 | 8355993 |
| synthesis and potential anthelmintic activity of methyl-5-(4-salicyloyl-piperazin-1-yl)-benzimidazole-2-carbamates. | a series of methyl-5-(4-salicyloyl-piperazin-1-yl)-benzimidazole-2-carba mates (ix) has been prepared. 5-(4-salicyloyl-piperazin-1-yl)-2-nitro anilines (vii) have been synthesized starting from 5-piperazino-2-nitro anilines and salicyloyl chlorides. catalytic reduction of vii with pd/c, followed by treatment with 1,3-dicarbomethoxy-s-methyl isothiourea, yielded the corresponding carbamates (ix), the anthelmintic activity of which was studied. compound ixa significantly reduced the numbers of pre ... | 1993 | 8352825 |
| trichinella spiralis: genetic basis and kinetics of the anti-encysted muscle larval response in miniature swine. | primary-infected swine lymphocyte antigen (sla) inbred miniature swine, nih minipigs, of the slaa/a haplotype (aa) display markedly reduced trichinella spiralis-encysted muscle larval (ml) burdens 6 weeks after homologous challenge and are therefore referred to as responders against encysted t. spiralis ml. to investigate the kinetics and genetics of the anti-encysted ml response, and in an attempt to ascertain the mechanisms underlying this phenomenon, nih minipigs of multiple sla haplotypes, d ... | 1993 | 8344404 |
| [the helminth fauna of wild boars of the west berlin forests]. | samples of muscles and internal organs obtained from 96 wild boars in the forests of west berlin between september 1989 and august 1990, were examined for helminths. nine nematode species, metastrongylus pudendotectus, m. salmi, m. confusus, m. apri, physocephalus sexalatus, ascarops strongylina, globocephalus urosubulatus, ascaris suum and trichuris suis belonging to five families could be found. larvae of toxocara spp. were isolated from most of the enzymatically digested samples of the gastro ... | 1993 | 8343108 |
| effects of maternal alcohol consumption on milk and blood lymphocytes and igg antibody levels from trichinella spiralis-infected rats. | immunity to the intestinal parasite trichinella spiralis can be transferred from the mother to the neonate during lactation. previous studies in our laboratory showed that the passage of immunity to pups from ethanol-treated dams was depressed. this study examined the effect of ethanol consumption during pregnancy and lactation on the t. spiralis-associated immune components in milk and blood. groups of female rats were fed either ethanol-containing or isocaloric liquid diets for 30 days before ... | 1993 | 8333580 |
| trichinella spiralis infection in pigs in the bolivian altiplano. | trichinella spiralis infection has been reported sporadically in several areas in central and south america. however, several countries, including bolivia, have not reported trichinellosis in animals or humans. a small survey of pigs slaughtered in an abattoir in bolivia was undertaken during september 1991, to determine the presence of trichinella spiralis. in a group of 100 pigs slaughtered consecutively on a single day and tested using the pooled digestion method, two of eight pools (25%) wer ... | 1993 | 8333140 |
| ultrastructural localization of the phosphorylcholine-associated antigen in trichinella spiralis. | two monoclonal antibodies that are specific for phosphorylcholine (pc) were used as probes in immunoelectron microscopy to locate the pc-associated antigen(s) in trichinella spiralis. this antigen was found to be present abundantly in various internal structures in the muscle larva and the adult worm, but it was virtually absent in the fetal/newborn larva. in the muscle larva, the major pool of pc was found in the inner layer of the body cuticle and in various organelles such as the "rapid-respo ... | 1993 | 8331481 |
| trichinella spiralis: immunity, ecology, and evolution. | parasites exploit the environment provided by their hosts in the same way that free-living animals exploit their physical and biotic environments. there is, however, 1 important difference: the host environment can respond adaptively to infection in ways that may be disadvantageous to the parasite. immunity provides the most effective adaptive host response and exerts a major selective pressure in parasite evolution. among parasites many ways of maintaining the ability to exploit the host in the ... | 1993 | 8331470 |
| trichinella spiralis and the concept of niche. | trichinella spiralis is an intracellular parasite as both a larva and an adult. the first-stage larva lives in a modified portion of a skeletal muscle cell, the nurse cell, and can reside there for the life span of the host. adult worms occupy a nonmembrane-bound portion of columnar epithelium, living there as intramulticellular parasites. the newborn larva is the only nonintracellular stage, living free in the circulation. trichinella spiralis induces modifications in each of its intracellular ... | 1993 | 8331468 |
| [biological and parasitic variations in horses infested and reinfested by trichinella spiralis]. | seven mares were infected with 20,000 trichinella spiralis larvae; 2 of them were reinfected 22 wk later with the same amount of larvae. the course of infection in horses was assessed by serology (elisa), biochemistry (aldolase activity), parasitology and histopathology. in each animal, infection was followed by a significant rise in specific antibody titers culminating at 5-10 wk post-infection (pi) and decreasing thereafter. reinfection was followed by a slight rise in antibody levels. aldolas ... | 1993 | 8319006 |
| influence of variation in host strain and parasite isolate on inflammatory and antibody responses to trichinella spiralis in mice. | variation in the immunogenicity of 3 isolates of trichinella spiralis was assessed by the parameters of adult worm recovery, mast cell, eosinophil and antibody responses in mice of defined response phenotype. the levels of the protective, inflammatory and immune responses induced by infection differed between the isolates. isolates showed considerable variation in the capacity to elicit mast cell and eosinophil responses. all induced increases in parasite-specific antibody, levels of total (igga ... | 1993 | 8316434 |
| identification of trichinella isolates from naturally infected stray dogs in egypt. | larvae of trichinella species recovered from the diaphragms of 2 stray dogs killed during a governmental antirabies campaign in cairo, egypt, were fed to white mice for production of adult worms and larvae for morphological and isoenzyme studies. comparisons were made with reference species of trichinella spiralis, trichinella nelsoni, trichinella nativa, and trichinella pseudospiralis. results indicated that the 2 trichinella specimens from the dogs were morphologically and biochemically identi ... | 1994 | 8308650 |
| biochemical characterisation of trichinella spiralis and t. pseudospiralis stichocyte antigens. | one and two dimensional western blots of trichinella spiralis and trichinella pseudospiralis extracts were probed with a monoclonal antibody (7c2c5) which recognizes molecules found in the stichocytes. three major antigens were revealed in excreted:secreted (e/s) preparations at approx mr 45, 53 & 60 x 10(3). the mr 45 x 10(3) molecule was the most abundant secreted antigen, on two dimensional analysis it resolved into many isoforms with a pi range 4.5-6.4 in t. spiralis and into four isoforms p ... | 1993 | 8298662 |