Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the cost-effectiveness of treating male trichomoniasis to avert hiv transmission in men seeking sexually transmitted disease care in malawi. | allocation of funds to program areas where they may have an impact is critical to the success of any hiv control program. we examined the cost-effectiveness of providing first-line treatment for male trichomoniasis in malawi, a condition not commonly considered in syndromic management throughout sub-saharan africa. | 2006 | 16951650 |
| two novel serum-free media for the culture of trichomonas vaginalis. | definitive diagnosis of trichomonas vaginalis, one of the common causes of sexually transmitted diseases in turkey, relies on the microscopic examination of both fresh preparations and culture material of genital secretions and urine. the aim of this study was to compare the diagnostic efficacies of two culture media, serum-free tb1 and tb2, including iron and vitamin b12, respectively, with the well-known medium, tym. growth rate reached peak levels 48 h in tym and 72 h in both tb1 and tb2 afte ... | 2007 | 16947014 |
| comparison between the gen-probe transcription-mediated amplification trichomonas vaginalis research assay and real-time pcr for trichomonas vaginalis detection using a roche lightcycler instrument with female self-obtained vaginal swab samples and male urine samples. | this study compared two assays for trichomonas vaginalis detection, gen-probe's transcription-mediated amplification (tma) assay for trichomonas vaginalis and btub fret pcr, using self-obtained clinical samples from 611 patients. infection status was defined as two positive results by two different tests. the initial tma assay sensitivity was 96.7%; specificity was 97.5%. the tma assay was comparable to btub fret pcr. | 2006 | 16943353 |
| realtime pcr on urine sediments in the diagnosis of trichomoniasis in male subjects. | 2006 | 16942660 | |
| [vaginal microbiocenosis in trichomoniasis]. | taxonomic complex of vaginal microflora and its persistence characteristics in trichomoniasis and microecological disturbances were studied. diminution of biotope colonization resistance was characterized by decreased autochthonous microorganisms' number and increased opportunistic microflora with expressed persistence. that could be of a great pathogenetic importance in the occurrence of reproductive tract inflammatory diseases of the bacterial etiology in addition of trichomoniasis in females. | 2006 | 16941883 |
| viability of trichomonas vaginalis in urine: epidemiologic and clinical implications. | the impact of the viability of trichomonas vaginalis in urine on wet mount, culture, and pcr methods was assessed. to minimize the chance of false-negative results, urine specimens should be processed within 30 min of specimen collection and maintained at 37 degrees c, since temperature appears to affect the viability of trichomonas. | 2006 | 16928970 |
| sexually transmitted and reproductive tract infections in symptomatic clients of pharmacies in lima, peru. | to determine prevalences and predictors of sexually transmitted and reproductive tract infections among men and women seeking care at pharmacies. | 2007 | 16916881 |
| trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase-3 and reducing mcl-1 expression. | neutrophils are the predominant inflammatory cells found in the vaginal discharge of patients with trichomonas vaginalis infection. however, it is not known whether neutrophil apoptosis is induced by live t. vaginalis. therefore, we examined whether t. vaginalis can influence neutrophil apoptosis, and also whether caspase-3 and the bcl-2 family members are involved in the apoptosis. thus, human neutrophils were incubated with live t. vaginalis and neutrophil apoptosis was evaluated by giemsa, an ... | 2006 | 16916367 |
| [hydrolytic activity of trichomonas vaginalis donné strains]. | the hydrolytic activity of trichomonas vaginalis donné strains was investigated using api zym test (biomérieux). among 19 determined hydrolases were found alkaline and acid phosphatases; also in several strains n-acetyl-beta-glucosaminidase and a-glucosidase were demonstrated. the api zym test may be used for characterization of enzymatic activity of t. vaginalis strains, but adequate medium is necessary. | 2001 | 16897946 |
| [prevalence of trichomonas vaginalis donné in women of lódź population in 1955-1999 years]. | search for trichomonas vaginalis was carried out in direct (in 0.85% nacl and 0.1% safranine) and fixed stained microscopical preparations ( wright or giemsa methods, after fixation with 70% methyl alcohol) and cultures ( pavlova, johanson-trussel-john, roiron-ratner, simic media), from materials of the vagina, urethra and cervix uteri. in the years 1955-1999 a total of about 12000 women were examined. the prevalence of t. vaginalis in women population of lódź region was high (from 26.6% still 7 ... | 2001 | 16897945 |
| trichomonas vaginalis in turkey--a review. | 2001 | 16897944 | |
| location of the cell-binding domain of cp65, a 65kda cysteine proteinase involved in trichomonas vaginalis cytotoxicity. | the cysteine proteinase (cp) of 65kda, cp65, binds to the surface of hela cells and is involved in trichomonas vaginalis cellular damage. to identify and locate the cp65 cellular-binding domain, we enriched the cp65 protein band by ammonium sulfate fractionation and ion-exchange chromatography and the n-terminal sequence was obtained. a 618bp gene fragment was obtained by pcr using genomic dna as template and primers derived from the n-terminal sequence of cp65 and the asn papain-catalytic conse ... | 2006 | 16891146 |
| genetic structure and evolution of the vps25 family, a yeast escrt-ii component. | vps25p is the product of yeast gene vps25 and is found in an endosomal sorting complex required for transport (escrt)-ii, along with vps22p and vps36p. this complex is essential for sorting of ubiquitinated biosynthetic and endosomal cargoes into endosomes. | 2006 | 16889659 |
| trichomonas vaginalis initiator binding protein (ibp39) and rna polymerase ii large subunit carboxy terminal domain interaction. | the core promoter that directs rna polymerase to the start of transcription in the protist trichomonas vaginalis is an initiator (inr) element recognized by the inr binding protein, ibp39. this nuclear protein is composed of two domains: a 14.5 kda amino (n-terminal) and a 25 kda carboxy terminal domain (c-domain). here we describe the identification of an ibp39-interacting protein by screening a t. vaginalis expression library using a two-hybrid system with the ibp39 c-domain as bait. the ctd o ... | 2006 | 16879883 |
| lack of effectiveness of syndromic management in targeting vaginal infections in pregnancy in entebbe, uganda. | to measure the prevalence of reproductive tract infections (rtis) during pregnancy in entebbe, uganda, and to evaluate the current syndromic diagnosis and management approach in effectively targeting infections, such as bacterial vaginosis (bv) and trichomoniasis, that are associated with low birth weight and prematurity among newborns. | 2006 | 16877576 |
| predicting antitrichomonal activity: a computational screening using atom-based bilinear indices and experimental proofs. | existing trichomonas vaginalis therapies are out of reach for most trichomoniasis people in developing countries and, where available, they are limited by their toxicity (mainly in pregnant women) and their cost. new antitrichomonal agents are needed to combat emerging metronidazole-resistant trichomoniasis and reduce the side effects associated with currently available drugs. toward this end, atom-based bilinear indices, a new tomocomd-cardd molecular descriptor, and linear discriminant analysi ... | 2006 | 16875830 |
| [construction of eukaryotic expression recombinant plasmid of trichomonas vaginalis ferredoxin gene]. | total dna was extracted from t. vaginalis with chelex-100 method and used as templates for pcr. the ferredoxin gene was directionally cloned into plasmid pmd-18t vector and subcloned into eukaryotic expression vector pcdna3. 1 (+). the transformants were screened and identified by pcr and restriction analysis. the size of amplified ferredoxin gene was 306bp and the dna sequence of cloned gene was same with that published. | 2006 | 16866153 |
| [preparation, characterization and preliminary application of recombinant protein ap33 of trichomonas vaginalis]. | to clone ap33 gene of trichomonas vaginalis( t. v), construct prokaryotic expression system of the gene and identify its antigenicity and immunogenicity. | 2006 | 16866139 |
| [detection of mycoplasma hominis in trichomonas vaginalis by pcr]. | twelve of 28 isolates of trichomonas vaginalis collected from patients in hospitals in guangzhou were found naturally infected with mycoplasma hominis by pcr using specific primers. it suggests that the symbiosis of m. hominis in t. vaginalis also commonly occurs in china. | 2006 | 16862916 |
| association of bacterial vaginosis, trichomonas vaginalis, and vaginal acidity with outcome of pregnancy. | bacterial vaginosis and trichomons vaginalis are believed to be the risk factors for preterm labor birth and preterm prelabor rupture of membranes. the objective of this study was to investigate the association between bacterial vaginosis, t.vaginalis, and vaginal ph with preterm labor birth and preterm prelabor rupture of membranes after excluding other known risk factors. | 2006 | 16859053 |
| symbiosis of mycoplasma hominis in trichomonas vaginalis may link metronidazole resistance in vitro. | fourteen of 28 trichomonas vaginalis isolates collected from patients in guangzhou, china from 2003 to 2004 were found to be naturally infected with mycoplasma hominis, as determined by pcr using specific primers. in vitro metronidazole sensitivity assay of the 28 isolates revealed four displaying low susceptibility [minimum lethal concentration (mlc)= approximately 13-25 microg/ml] and another four displaying high resistance (mlc=50-100 microg/ml). the overwhelming majority of these resistant i ... | 2006 | 16847608 |
| is the presence of trichomonas on a pap smear associated with an increased incidence of bacterial vaginosis? | bacterial vaginosis (bv) is associated with significant morbidity. bacterial vaginosis is due to an overgrowth of anaerobic organisms in the vagina. it has been postulated that the presence of trichomonas creates an anaerobic environment that favors bv. thus, bv should be more frequent in the presence of trichomonas if trichomonas is creating a favorable anaerobic environment. | 2006 | 16829751 |
| sociodemographic markers and behavioral correlates of sexually transmitted infections in a nonclinical sample of adolescent and young adult women. | we examined sociodemographic markers and 3-month behavioral correlates of sexually transmitted infections (stis) in a nonclinical cross-section of adolescent and young adult women. | 2006 | 16826478 |
| trichomonas vaginalis and trichomoniasis in the republic of korea. | vaginal trichomoniasis, caused by trichomonas vaginalis, is the most common sexually transmitted disease. more than 170 million people worldwide are annually infected by this protozoan. in the republic of korea, 10.4% of women complaining of vaginal symptoms and signs were found to be infected with t. vaginalis. however, despite its high prevalence, the pathogenesis of t. vaginalis infection has not been clearly characterized although neutrophil infiltration is considered to be primarily respons ... | 2006 | 16809958 |
| synthesis of benzenepropanamine analogues as non-detergent spermicides with antitrichomonas and anticandida activities. | fifteen analogues of benzenepropanamine were synthesized and evaluated for their spermicidal as well as microbicidal activities against trichomonas vaginalis and candida spp. several compounds showed appreciable dual activities. compound 12 exhibited good spermicidal (mec=0.1%) along with substantial anticandidal (mic=0.05%) activities, while compounds 3 and 6 showed significant microbicidal activities with moderate spermicidal effect. the sar of these structures is being discussed here in this ... | 2006 | 16793275 |
| lack of evidence for the involvement of rectal and oral trichomonads in the aetiology of vaginal trichomoniasis in ghana. | to investigate the possible involvement of human trichomonads (pentatrichomonas hominis and trichomonas tenax) other than trichomonas vaginalis in the aetiology of vaginal trichomoniasis. | 2007 | 16790560 |
| cross-reactivity of trichomonas in cervical p16ink4a immunocytochemistry is dependent on antibody, not antigen. | 2006 | 16783782 | |
| interaction between trichomonas vaginalis and human spermatozoa in the female genital tract: papanicolaou-stained cervical smear findings. | 2006 | 16780038 | |
| dependence on p38 mapk signalling in the up-regulation of tlr2, tlr4 and tlr9 gene expression in trichomonas vaginalis-treated hela cells. | toll-like receptors (tlrs) are pattern recognition receptors (prrs) that recognize conserved pathogen-associated molecular patterns (pamps) synthesized by micro-organisms. despite the essential requirement for tlrs in prokaryotic infection, the pattern and regulation of tlr gene expression by trichomonas vaginalis in the mucocutaneous barrier are still unknown. our hypothesis is that t. vaginalis-infected epithelial cells are major effector cells in the skin barrier. these cells function as a ce ... | 2006 | 16771851 |
| the vi-sense-vaginal discharge self-test to facilitate management of vaginal symptoms. | this study was undertaken to evaluate a diagnostic panty liner (vi-sense) (common sense, caesarea, israel) developed to facilitate diagnosis of vaginal infections by detecting disordered acidity level. | 2006 | 16769019 |
| alfred donné and léon foucault: the first applications of electricity and photography to medical illustration. | the first electric projecting microscope and the first published photomicrographs harnessed recent technological developments to provide improved methods of medical illustration for educational purposes. both projects were the work of two frenchmen. the impetus came from the microscopist dr alfred donné (1801-78), discoverer of trichomonas vaginalis and leukaemia. implementation was primarily by his medical-student assistant, léon foucault (1819-68), who later gained fame as a physicist, especia ... | 2006 | 16766307 |
| a community-based study of risk factors for trichomonas vaginalis infection among women and their male partners in moshi urban district, northern tanzania. | the objective of this study was to determine predictors of trichomonas vaginalis among women and their partners in moshi, tanzania. | 2006 | 16755271 |
| incidence and correlates of unwanted sex in relationships of middle and late adolescent women. | to determine the 3-month incidence of unwanted sex and to examine relationship factors and health-risk behaviors associated with incident unwanted sex. | 2006 | 16754820 |
| antiparasitic activity of two lavandula essential oils against giardia duodenalis, trichomonas vaginalis and hexamita inflata. | two essential oils derived from lavandula angustifolia and lavandula x intermedia were investigated for any antiparasitic activity against the human protozoal pathogens giardia duodenalis and trichomonas vaginalis and the fish pathogen hexamita inflata: all of which have significant infection and economic impacts. the study has demonstrated that low (< or = 1%) concentrations of l. angustifolia and l. x intermedia oil can completely eliminate t. vaginalis, g. duodenalis and h. inflata in vitro. ... | 2006 | 16741725 |
| cysteine biosynthesis in trichomonas vaginalis involves cysteine synthase utilizing o-phosphoserine. | trichomonas vaginalis is an early divergent eukaryote with many unusual biochemical features. it is an anaerobic protozoan parasite of humans that is thought to rely heavily on cysteine as a major redox buffer, because it lacks glutathione. we report here that for synthesis of cysteine from sulfide, t. vaginalis relies upon cysteine synthase. the enzyme (tvcs1) can use either o-acetylserine or o-phosphoserine as substrates. the k(m) values of the enzyme for sulfide are very low (0.02 mm), sugges ... | 2006 | 16735516 |
| microscopy and culture for trichomonas vaginalis: are both required? | many genitourinary medicine clinics have stopped routinely performing both wet preparation microscopy and cultures to diagnose trichomonas vaginalis (tv). our directorate stopped microscopy when screening asymptomatic women. this audit considers whether both tests continue to be warranted for symptomatic female patients. the discrepancy between microscopy and culture results leads us to recommend that both remain necessary. sampling standardization and improved documentation are discussed. | 2006 | 16734968 |
| readily treatable reproductive tract infections and preterm birth among black women. | this study sought to quantify the risks of preterm birth that are ascribable to potentially treatable reproductive tract infections among black women in denver, colorado. | 2006 | 16731091 |
| mycoplasma hominis and trichomonas vaginalis: a unique case of symbiotic relationship between two obligate human parasites. | mollicutes are the smallest and simplest self-replicating microorganisms. despite the minimal genome and apparent lack of complexity, mycoplasmas show a high degree of adaptation to the most diverse environments. mycoplasma hominis is a human sexually transmitted mycoplasma which is able to establish a biological association with trichomonas vaginalis, a pathogenic flagellated protist. m. hominis and t. vaginalis share the same specific natural niche, the human genitourinary tract. symbiotic rel ... | 2006 | 16720288 |
| plasma antibodies against trichomonas vaginalis and subsequent risk of prostate cancer. | although several previous case-control studies have investigated associations between sexually transmitted infections (sti) and prostate cancer, most have focused on gonorrhea and syphilis, two well-recognized, symptomatic stis. another sti of interest for prostate carcinogenesis is trichomonosis, a less well recognized and frequently asymptomatic sti with known prostate involvement. we investigated this infection in relation to incident prostate cancer in a nested case-control study within the ... | 2006 | 16702374 |
| genotyping trichomonas vaginalis. | a genotyping method has been developed to distinguish each trichomonas vaginalis isolate and has provided the first genome mapping studies of this protist with an estimated 180mb genome. the technique was developed using high molecular weight dna prepared from five laboratory isolates from australia and usa and 20 clinical isolates from south africa. inhibition of the notorious t. vaginalis endogenous nucleases by addition of potent inhibitors was essential to the success of this study. chromoso ... | 2006 | 16698025 |
| development of a nonhuman primate model for trichomonas vaginalis infection. | trichomoniasis, caused by trichomonas vaginalis, is a prevalent sexually transmitted infection associated with increased risk of hiv infection. an animal model of t. vaginalis infection would enable scientists to further investigate trichomoniasis. | 2006 | 16691156 |
| the associations between pelvic inflammatory disease, trichomonas vaginalis infection, and positive herpes simplex virus type 2 serology. | roles for chlamydia trachomatis and neisseria gonorrhoeae infections in pelvic inflammatory disease pathogenesis are well delineated; however, the etiologic contributions of herpes simplex virus type 2 (hsv-2) and trichomonas vaginalis have been underexplored. | 2006 | 16691155 |
| cervical smear participation and prevalence of sexually transmitted infections in women attending a community-controlled indigenous health service in north queensland. | to increase pap smear participation and to assess the prevalence of sexually transmitted infection (sti) in urban indigenous women in townsville. | 2006 | 16681340 |
| cytotoxic potential of cervical mucus on trichomonas vaginalis to prevent upper genital tract infections. | 2006 | 16680275 | |
| variation in vaginal immune parameters and microbial hydrolytic enzymes in bacterial vaginosis positive pregnant women with and without mobiluncus species. | this study was undertaken to assess if levels of interleukin-1beta (il-1beta), il-8, sialidase, prolidase and immunoglobulin a against gardenerella vaginalis hemolysin (anti-gvh iga) in vaginal secretions differ between bv+ women with (m+) and without (m-) mobiluncus spp. | 2006 | 16643824 |
| use of trichomonas vaginalis clinical isolates to evaluate correlation of gene expression and metronidazole resistance. | we investigated whether variations in gene expression of enzymes associated with anaerobic resistance of laboratory-derived strains of trichomonas vaginalis could be detected in a group of 28 clinical isolates with variations in metronidazole sensitivity. we compared isolates by real-time pcr because this method allows for highly sensitive quantification of mrna and for evaluation of several genes simultaneously. we found that pfor gene a mrna levels were highly correlated with pfor gene b level ... | 2006 | 16629339 |
| hydrogenosomes and symbiosis. | 2006 | 16623392 | |
| examination of urine for bacteria and parasites among elder persons in the rural area, in dammam district. | the asymptomatic bacteria and parasites in urines were studied in elder persons living in the rural areas of dammam district. complete history taking, clinical examination, urine analysis and culture, blood glucose level and haemoglobins in gm were performed for 43 elders of both sexes. the bacteria detected in urine in a descending order were klebsiella sp. staphyloccocus sp., escherichia coli and pseudomonas sp. the parasites detected were only trichomonas vaginalis in three diabetic females. ... | 2006 | 16605119 |
| [gynaecological and obstetrical aspects of recurrent urinary tract infections]. | the microbial colonization of vulva, vagina and cervix uteri represents the reservoir for recurrent urinary tract infection. all bacterial species of normal cutaneous or gastrointestinal flora can be found in the external genital tract even under physiological conditions. the higher concentration of microbes adds to the predisposition for urinary tract infection in cases of dysbiosis or inflammation, apart from specific infection by trichomonas vaginalis, neisseria gonorrhoeae or chlamydia trach ... | 2006 | 16586054 |
| two methods of self-sampling compared to clinician sampling to detect reproductive tract infections in gugulethu, south africa. | to assess the validity, feasibility, and acceptability of 2 methods of self-sampling compared to clinician sampling during a speculum examination. | 2006 | 16572041 |
| [rna interference to the expression of peroxiredoxin-related genes in trichomonas vaginalis]. | to inhibit the expression of the target genes of peroxiredoxin (prx) and thioredoxin reductase (trxr) by rna interference and evaluate its effect on the growth of trichomonas vaginalis. | 2005 | 16566216 |
| the nutrition of protozoa: iii. an improved procedure for separating human blood serum into the two fractions essential for the sustained growth of trichomonas vaginalis. | 1948 | 16561445 | |
| the nutrition of protozoa: ii. the separation of human blood serum into two fractions, both essential for the sustained growth of trichomonas vaginalis. | 1947 | 16561292 | |
| the nutrition of protozoa: i. a simplified medium for the investigation of unknown factors in blood serum essential for the sustained growth of trichomonas vaginalis. | 1947 | 16561291 | |
| sexually transmitted infections among pregnant women attending an antenatal clinic in fuzhou, china. | to determine the prevalence of sexually transmitted infections (stis) among women attending an antenatal clinic in urban china and to show whether reported symptoms and findings on clinical examination predicted stis in this population. | 2006 | 16554698 |
| evolution of four gene families with patchy phylogenetic distributions: influx of genes into protist genomes. | lateral gene transfer (lgt) in eukaryotes from non-organellar sources is a controversial subject in need of further study. here we present gene distribution and phylogenetic analyses of the genes encoding the hybrid-cluster protein, a-type flavoprotein, glucosamine-6-phosphate isomerase, and alcohol dehydrogenase e. these four genes have a limited distribution among sequenced prokaryotic and eukaryotic genomes and were previously implicated in gene transfer events affecting eukaryotes. if our pr ... | 2006 | 16551352 |
| patient-delivered partner treatment for trichomonas vaginalis infection: a randomized controlled trial. | infections with trichomonas vaginalis (tv) are common and recurrence rates are high. better methods of treating partners of women with trichomoniasis are needed. | 2006 | 16531939 |
| nitric oxide radicals in leucocytes and vaginal washes of trichomonas vaginalis -infected symptomatic and asymptomatic women. | the clinical spectrum of trichomonas vaginalis infection varies from asymptomatic to mild, moderate or severe vaginitis. nitric oxide and other reactive nitrogen radicals produced by immune effector cells are important cytotoxic and cytostatic mediators against several microorganisms including parasites. in the present study, inducible nitric oxide synthase (inos) and reactive nitrogen intermediates (rni) were determined in leucocyte cultures (stimulated with t. vaginalis in vitro) and vaginal w ... | 2006 | 16529664 |
| fe-hydrogenase maturases in the hydrogenosomes of trichomonas vaginalis. | assembly of active fe-hydrogenase in the chloroplasts of the green alga chlamydomonas reinhardtii requires auxiliary maturases, the s-adenosylmethionine-dependent enzymes hydg and hyde and the gtpase hydf. genes encoding homologous maturases had been found in the genomes of all eubacteria that contain fe-hydrogenase genes but not yet in any other eukaryote. by means of proteomic analysis, we identified a homologue of hydg in the hydrogenosomes, mitochondrion-related organelles that produce hydro ... | 2006 | 16524912 |
| hydrogenosomal succinyl-coa synthetase from the rumen-dwelling fungus neocallimastix patriciarum; an energy-producing enzyme of mitochondrial origin. | hydrogenosomes are hydrogen-producing organelles that are related to mitochondria and found in a variety of evolutionarily unrelated anaerobic microbial eukaryotes. similar to classic mitochondria, hydrogenosomes contain the enzyme catalyzing the only reaction of the citric acid cycle directly producing energy; succinyl-coa synthetase. we have isolated and characterized the genes encoding both subunits of this enzyme from the anaerobic chytrid fungus neocallimastix patriciarum, a model organism ... | 2006 | 16515848 |
| split-sample analysis of discarded cells from liquid-based pap smear sampling devices. | to examine cells that were retained on sampling devices used to collect thinprep (cytyc corp., boxborough, massachusetts, u.s.a) pap smears in order to evaluate both the number and significance of cells that are routinely discarded with these devices after liquid-based specimens are collected. | 2006 | 16514841 |
| association of genital shedding of herpes simplex virus type 2 and hiv-1 among sex workers in rural zimbabwe. | herpes simplex virus type 2 (hsv-2) facilitates sexual acquisition of hiv-1 but data on transmission are less clear. in this study the interaction between genital shedding of hiv-1 and hsv-2 was explored among zimbabwean sex workers. | 2006 | 16511420 |
| structure of citrobacter freundii l-methionine gamma-lyase. | l-methionine gamma-lyase (mgl) is a pyridoxal 5'-phosphate (plp) dependent enzyme that catalyzes gamma-elimination of l-methionine. the crystal structure of mgl from citrobacter freundii has been determined at 1.9 a resolution. the spatial fold of the protein is similar to those of mgls from pseudomonas putida and trichomonas vaginalis. the comparison of these structures revealed that there are differences in plp-binding residues and positioning of the surrounding flexible loops. | 2005 | 16511092 |
| throwing the dice for the diagnosis of vaginal complaints? | vaginitis is among the most common conditions women are seeking medical care for. although these infections can easily be treated, the relapse rate is high. this may be due to inadequate use of the diagnostic potential. | 2006 | 16503990 |
| prevalence of sexually transmitted infections among adolescents in kampala, uganda, and theoretical models for improving syndromic management. | to estimate the prevalence of treatable sexually transmitted infections (sti) in adolescents visiting a youth health clinic. to evaluate the algorithm for management of the abnormal vaginal discharge (avd) syndrome recommended in uganda's national guidelines and compare it with other theoretical flowchart models. | 2006 | 16488818 |
| phylogenetic positions of several amitochondriate protozoa--evidence from phylogenetic analysis of dna topoisomerase ii. | several groups of parasitic protozoa, as represented by giardia, trichomonas, entamoeba and microsporida, were once widely considered to be the most primitive extant eukaryotic group--archezoa. the main evidence for this is their 'lacking mitochondria' and possessing some other primitive features between prokaryotes and eukaryotes, and being basal to all eukaryotes with mitochondria in phylogenies inferred from many molecules. some authors even proposed that these organisms diverged before the e ... | 2005 | 16483135 |
| results of longterm hospital based cytological screening in asymptomatic women. | routine cytological screening has been carried out in 27,062 asymptomatic women attending gynaec and family planning o.p.d. of queen mary's hospital, lucknow, india (april 1971-december 2004). incidence of squamous intraepithelial lesion (sil) was found to be 5.9% in the series, while cervical malignancy was seen in 0.6% of cases. the study highlighted the immense utility of cytological screening in minimizing the incidence of carcinoma cervix in the segment of the urban population screened, as ... | 2006 | 16470854 |
| decline in sexually transmitted infection prevalence and hiv incidence in female barworkers attending prevention and care services in mbeya region, tanzania. | to assess trends in sexually transmitted infection (sti) prevalence and hiv incidence and associated factors in a cohort of female barworkers exposed to behavioural interventions and sti screening and treatment. | 2006 | 16470126 |
| multifarious transcriptional regulation of adhesion protein gene ap65-1 by a novel myb1 protein in the protozoan parasite trichomonas vaginalis. | the transcription efficiency of an adhesion protein gene, ap65-1, in trichomonas vaginalis varies with changes in the iron supply and with the growth stage. in the present study, two myb recognition elements, mre-1/mre-2r and mre-2f, were found to play antagonistic roles in regulating the iron-inducible activity of an ap65-1 reporter gene. intriguingly, either of these elements was shown to be sufficient to repress basal activity, but together they were also shown to activate growth-related acti ... | 2006 | 16467479 |
| the spermicidal and antitrichomonas activities of ssri antidepressants. | the study investigated spermicidal and antitrichomonas activities of selective serotonin reuptake inhibitor (ssri) antidepressants with a view to generate new lead for development of dual-function spermicidal microbicides, which is an urgent global need. fluoxetine, sertraline, and fluvoxamine exhibited both spermicidal and anti-sti (antitrichomonas) activities in vitro, whereas paroxetine and citalopram showed only the spermicidal activity. fluoxetine exhibited better activity profile than the ... | 2006 | 16464584 |
| persistent increase in the incidence of acute male urethritis diagnosed in general practices in france. | at-risk sexual behaviour seems to have increased in europe, possibly due to the reassuring efficacy of highly active antiretroviral treatments. | 2006 | 16464324 |
| [exogenous and endogenous infections of genital tract]. | the paper is an overview of etiological factors, clinical manifestations, diagnostics and treatment of genital tract infections. infections caused by trichomonas vaginalis, fungi candida, saccharomyces and geotrichum are discussed as well as syphilis, gonorrhea and infections with chlamydia, mycoplasms and ureaplasms. the problem of vaginal dysbacteriosis and mixed infections is pointed out. | 2005 | 16463706 |
| association of condom use, sexual behaviors, and sexually transmitted infections with the duration of genital human papillomavirus infection among adolescent women. | to examine the association of potentially modifiable factors such as condom use, sexual behaviors, and concurrent sexually transmitted infections with duration of genital human papillomavirus (hpv) infections among adolescent women. | 2006 | 16461870 |
| midpregnancy genitourinary tract infection with chlamydia trachomatis: association with subsequent preterm delivery in women with bacterial vaginosis and trichomonas vaginalis. | the objective of the study was to estimate whether midpregnancy genitourinary tract infection with chlamydia trachomatis is associated with an increased risk of subsequent preterm delivery. | 2006 | 16458652 |
| use of an adaptation of a commercially available pcr assay aimed at diagnosis of chlamydia and gonorrhea to detect trichomonas vaginalis in urogenital specimens. | trichomonas vaginalis pcr using reagents from a commercially available assay for chlamydia trachomatis and neisseria gonorrhoeae was evaluated for detection of infection in women and men attending a sexually transmitted disease clinic. evaluations included three primer sets, endocervical swabs, vaginal swabs and urine, and various storage conditions. the tvk3/tvk7 primer set was optimal in our hands with sensitivities ranging from 69.5 to 96.8%. in all comparisons, t. vaginalis pcr performed bet ... | 2006 | 16455885 |
| the frequency of sister chromatid exchanges in cultured human peripheral blood lymphocyte treated with metronidazole in vitro. | 5-nitroimidazoles are a group of antiprotozoal and antibacterial agents. thanks to their antimicrobial activity, these chemotherapeutic agents inhibit the growth of both anaerobic bacteria and certain anaerobic protozoa. one of the useful drugs used in the treatment of infections caused by trichomonas vaginalis, entamoeba histolytica, and giardia lamblia is metronidazole (mtz). the mutagenicity of metronidazole [1-(hydroxyethyl)-2-methyl-5-nitroimidazole] has been previously shown in different p ... | 2006 | 16455592 |
| microbicidal spermicide or spermicidal microbicide? | vaginal contraception, the oldest method of fertility regulation that remained virtually forgotten for a few decades has recently come under focal review due to an increase in stds and hiv infections worldwide. today it is being considered very strongly that a conceptual microbicidal spermicide can tender protection against pregnancy as well as stds (including aids), simultaneously. however the two activities (spermicidal and microbicidal) need to be integrated in vaginal preparations, as many w ... | 2005 | 16448947 |
| a novel surface protein of trichomonas vaginalis is regulated independently by low iron and contact with vaginal epithelial cells. | trichomonosis caused by trichomonas vaginalis is the number one, non-viral sexually transmitted disease (std) that affects more than 250 million people worldwide. immunoglobulin a (iga) has been implicated in resistance to mucosal infections by pathogens. no reports are available of iga-reactive proteins and the role, if any, of this class of antibody in the control of this std. the availability of an iga monoclonal antibody (mab) immunoreactive to trichomonads by whole cell (wc)-elisa prompted ... | 2006 | 16448556 |
| [the implication of trichomoniasis and giardiasis in the development of inflammatory diseases of small pelvic organs]. | 2005 | 16445233 | |
| trichomoniasis and its treatment. | trichomonas vaginalis has long been recognized as a cause of infectious vaginitis in women. more recently, studies have demonstrated a significant burden of disease in men with urethritis or men at high risk for sexually transmitted diseases. there is increasing interest in this pathogen as more data accumulates linking it to hiv transmission and perinatal morbidity. new diagnostic methods have emerged that may increase sensitivity of diagnosis or improve point-of-care access to testing. nitroim ... | 2006 | 16441214 |
| consideration for empiric trichomonas vaginalis treatment among selected high-risk hiv-infected female populations with concurrent neisseria gonorrhea or chlamydia trachomatis cervical infections. | 2006 | 16432485 | |
| high-resolution structure of recombinant trichomonas vaginalis thioredoxin. | the structure of thioredoxin from the anaerobic organism trichomonas vaginalis (tvtrx) has been determined at 1.9 angstroms resolution. the structure is that of a typical thioredoxin: a five-stranded beta-sheet structure with two alpha-helices on either side. the active site of the protein carries a trp-cys-gly-pro-cys motif, residues 34-38, at the n-terminus of an alpha-helix (alpha2). the cysteine residues in this motif form a redox-active disulfide necessary for thioredoxin activity. with hig ... | 2006 | 16421453 |
| isolation, identification and sensitivity pattern of microorganisms isolated from the urine of pregnant women. | the present studies were conducted to detect and identify the microorganism from the urine of pregnant women having urinary tract infection. the antibiotic susceptibility of these isolated microorganisms was also determined. the microorganisms found responsible for the infection were bacteria, fungi, yeast and protozoa. among the bacteria two were identified as gram-positive cocci i.e. staphylococcus aureus and s. epidermidis, the remaining two were gram-negative bacilli which were escherichia c ... | 1994 | 16414749 |
| trichomonas vaginalis: paradigm of a successful sexually transmitted organism. | trichomonas vaginalis (tv) is one of the most successful protozoan pathogens and one of the most common sexually transmitted organism in females, yet it is also one of the most poorly investigated. by producing a wide array of glycosidases and cysteine proteinase enzymes, the organism can easily adapt to the environment, harvesting host proteins and dna for metabolism. with the ability to cause lesions, vaginitis and acute inflammatory disease of the genital mucosa, tv acts as a potential cataly ... | 2005 | 16411380 |
| double-stranded rna viral infection in cuban trichomonas vaginalis isolates. | trichomonas vaginalis can be infected with double-stranded rna (dsrna) viruses designated t. vaginalis virus (tvv), which may have important implications for trichomonal virulence and disease pathogenesis. we tested for tvv in 40 fresh t. vaginalis isolates from cuban patients by total extraction of nucleic acids (dna and rna). tvv was detected in 22 (55%) of the 40 t. vaginalis isolates. this gives an estimate of the infection rate of cuban t. vaginalis isolates by the dsrna virus. future resea ... | 2006 | 16410949 |
| bacterial vaginosis in female sex workers in chennai, india. | bacterial vaginosis (bv) causes obstetric and gynaecological complications and non-chlamydial/non-gonococcal pelvic inflammatory disease and has been shown to be associated with the risk of acquiring hiv and herpes simplex (hsv)-2 infections. this study investigated both the prevalence of bv and its association with stds among 582 female sex workers living in chennai, south india. blood, vaginal and endocervical swabs were tested for hsv-2, hiv, treponema pallidum, bv, chlamydia trachomatis, nei ... | 2005 | 16402677 |
| sexually transmitted infections, blood-borne viruses and risk behaviour in an australian senior high school population--the shlirp study. | to determine the feasibility and acceptability of screening for sexually transmitted infections and blood-borne viruses and to study the profile of sexual activity and other risk behaviours in a senior high school population. | 2005 | 16402670 |
| pyruvate-phosphate dikinase of oxymonads and parabasalia and the evolution of pyrophosphate-dependent glycolysis in anaerobic eukaryotes. | in pyrophosphate-dependent glycolysis, the atp/adp-dependent enzymes phosphofructokinase (pfk) and pyruvate kinase are replaced by the pyrophosphate-dependent pfk and pyruvate phosphate dikinase (ppdk), respectively. this variant of glycolysis is widespread among bacteria, but it also occurs in a few parasitic anaerobic eukaryotes such as giardia and entamoeba spp. we sequenced two genes for ppdk from the amitochondriate oxymonad streblomastix strix and found evidence for ppdk in trichomonas vag ... | 2006 | 16400177 |
| premature with trichomoniasis: report of one case. | we report a case of an extremely-low-birth-weight premature infant with trichomonas vaginalis infection of the vagina and urinary tract. her mother is also infected with trichomonas vaginalis but is asymptomatic. the patient's illness started as an asymptomatic pyuria and later on at early infancy developed profuse malodorous vaginal discharge. her vaginal discharge was positive for trichomonas vaginalis, both on wet mount and papanicolaou smear, and the infection responded well to treatment wit ... | 2005 | 16381336 |
| 5-nitroimidazole drugs effective against metronidazole-resistant trichomonas vaginalis and giardia duodenalis. | metronidazole (mz)-resistant giardia and trichomonas were inhibited by 1 of 30 new 5-nitroimidazole drugs. another five drugs were effective against some but not all of the mz-resistant parasites. this study provides the incentive for the continued design of 5-nitroimidazole drugs to bypass cross-resistance among established 5-nitromidazole antiparasitic drugs. | 2006 | 16377707 |
| sexually transmitted infections among female sex workers in yunnan, china. | a cross-sectional prevalence survey of sexually transmitted infections (stis) was conducted among female sex workers (fsws) in kunming, yunnan province, china. a total of 505 fsws participated in the survey. all eligible participants gave informed consent. demographic, behavioral, and clinical information of the participants was gathered by direct structured interviews. tampon swabs were collected to test for chlamydia trachomatis, nesseria gonorrhoeae, and trichomonas vaginalis and serum specim ... | 2005 | 16375618 |
| prevalence of trichomonas vaginalis infection in and around surat. | trichomonas culture method was used for the diagnosis of trichomoniasis along with gram staining and wet mount preparation. we selected 51 urban women and 51 rural women in this study. samples were taken from both symptomatic and asymptomatic individuals. out of 102 samples 35 samples were positive for trichomonas vaginalis by culture method. 20 women were positive for trichomonas vaginalis by wet mount and 15 by gram staining technique. so it is a fact that culture of trichomonas vaginalis is a ... | 2005 | 16366122 |
| apoptosis of macrophages induced by trichomonas vaginalis through the phosphorylation of p38 mitogen-activated protein kinase that locates at downstream of mitochondria-dependent caspase activation. | trichomonas vaginalis, a flagellated protozoan parasite, is the causative organism of trichomoniasis. we have recently demonstrated that t. vaginalis induces apoptotic cell death via a bcl-x(l)-dependent pathway in raw264.7 macrophages. in this study, we attempted to characterize in detail the signaling cascades resulting in t. vaginalis-induced macrophage apoptosis, focusing particularly on mitochondrial changes and the role of p38 mitogen-activated protein kinase (p38 mapk) activation. we foun ... | 2006 | 16360334 |
| effect of a killer toxin of yeast on eucaryotic systems. | the saccharomyces cerevisiae killer toxin kt 28, which inhibits sensitive yeasts, was shown to have no effect on several pathogenic fungi or on the protozoan trichomonas vaginalis. at concentrations of about 0.1 mg/ml, a partial inhibition of the skin pathogenic fungi trichophyton rubrum and microsporum canis was observed at ph 6.5. no pharmacological activity was detected in various tests with several animal organs. | 1988 | 16347605 |
| in vitro activity of hexadecylphosphocholine (miltefosine) against metronidazole-resistant and -susceptible strains of trichomonas vaginalis. | trichomonas vaginalis is the causative agent of trichomoniasis, a sexually transmitted disease with worldwide significance. trichomoniasis can be treated with metronidazole; however, resistant strains of t. vaginalis have been isolated and there is a lack of useful alternative drugs. the aim of the present study was to examine the activity of hexadecylphosphocholine (hepc; miltefosine), a membrane-active alkylphospholipid, that is licensed as an antileishmanial agent against t. vaginalis. | 2006 | 16344287 |
| reducing risk exposures to zero and not having multiple partners: findings that inform evidence-based practices designed to prevent std acquisition. | our objective was to assess prospectively the relative contribution of reducing penile-vaginal risk exposure to zero and limiting the number of sex partners to one, on the acquisition of biologically confirmed sexually transmitted disease (std) among african american women adolescents. data from a prospective cohort of 522 african american women adolescents enrolled in an hiv prevention trial were used. baseline std testing and single-dose directly observable treatment provided an infection-free ... | 2005 | 16336765 |
| comparison of conventional testing to polymerase chain reaction in detection of trichomonas vaginalis in indigenous women living in remote areas. | there are high rates of trichomonas vaginalis in remote areas of central australia. conventional tests for t. vaginalis have low sensitivity in this setting. aims of the study were to estimate the prevalence of t. vaginalis, to assess the presence of clinical signs and symptoms, to compare a t. vaginalis polymerase chain reaction (pcr) test with conventional methods of diagnosis, and to compare the pcr from different samples, including self-collected swabs (scs). of 205 women recruited, the prev ... | 2005 | 16336764 |
| evaluation of cervical smears at king hussein medical centre, jordan, over three and a half years. | cervical smears taken from women referred for a check-up or with vaginal itching/discharge over a period of 3.5 years were reviewed at the king hussein medical centre, jordan. all smears were fixed with 96% alcohol, stained with papanicolaou stain and screened microscopically. of the smears from 1176 women aged 18-70 years, 4.5% were classified as inadequate, 7.7% were normal and 79.9% showed non-specific inflammation. abnormal vaginal flora was found in 4.8% of cases, candida albicans in 1.2%, ... | 2004 | 16335662 |
| diagnosis of sexually transmitted infections (sti) using self-collected non-invasive specimens. | paramount in control of transmission of sexually transmitted infections (stis) is their prompt recognition and appropriate treatment. in countries where definitive diagnoses are difficult, a 'syndromic approach' to management of stis is recommended and practiced, yet many stis have common symptoms or are asymptomatic and therefore go undetected and untreated. this is of particular concern with the recognition that hiv transmission is increased with co-existent stis: the attributable risk for eac ... | 2004 | 16334994 |
| [the correlation of inflammation and epithelial changes in the pap smears of cervix uteri]. | a modification of the bethesda system classification of cytologic findings of uterine cervix named zagreb 1990 has been accepted in croatia as a unique classification, the use of which has begun after the publication and printing of the new, uniform method of cytologic examination. aim: the aim of this study was to assess the number and type of cytologic abnormalities of uterine cervix classified according to zagreb 1990, on routinely examined pap smears during the 6-year period, and to investig ... | 2005 | 16334735 |