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deep transcriptome sequencing provides new insights into the structural and functional organization of the wheat genome.because of its size, allohexaploid nature, and high repeat content, the bread wheat genome is a good model to study the impact of the genome structure on gene organization, function, and regulation. however, because of the lack of a reference genome sequence, such studies have long been hampered and our knowledge of the wheat gene space is still limited. the access to the reference sequence of the wheat chromosome 3b provided us with an opportunity to study the wheat transcriptome and its relati ...201525853487
isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion.the present study was conducted to characterize the native plant growth promoting (pgp) bacteria from wheat rhizosphere and root-endosphere in the himalayan region of rawalakot, azad jammu and kashmir (ajk), pakistan. nine bacterial isolates were purified, screened in vitro for pgp characteristics and evaluated for their beneficial effects on the early growth of wheat (triticum aestivum l.). among nine bacterial isolates, seven were able to produce indole-3- acetic acid in tryptophan-supplemente ...201525852661
physical mapping of chromosome 4j of thinopyrum bessarabicum using gamma radiation-induced aberrations.gamma radiation induced a series of structural aberrations involving thinopyrum bessarabicum chromosome 4j. the aberrations allowed for deletion mapping of 101 4j-specific markers and fine mapping of blue-grained gene bathb. irradiation can induce translocations and deletions to assist physically locating genes and markers on chromosomes. in this study, a 12-gy dosage of (60)co-γ was applied to pollen and eggs of a wheat (triticum aestivum) landrace chinese spring (cs)-thinopyrum bessarabicum ch ...201525851001
genome-wide dart and snp scan for qtl associated with resistance to stripe rust (puccinia striiformis f. sp. tritici) in elite icarda wheat (triticum aestivum l.) germplasm.identified dart and snp markers including a first reported qtl on 3as, validated large effect apr on 3bs. the different genes can be used to incorporate stripe resistance in cultivated varieties. stripe rust [yellow rust, caused by puccinia striiformis f. sp. tritici (pst)] is a serious disease in wheat (triticum aestivum). this study employed genome-wide association mapping (gwam) to identify markers linked to stripe rust resistance genes using diversity arrays technology (dart(®)) and single-n ...201525851000
composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in triticum urartu.wheat (aabbdd, 2n = 6x = 42) is a major dietary component for many populations across the world. bread-making quality of wheat is mainly determined by glutenin subunits, but it remains challenging to elucidate the composition and variation of low-molecular-weight glutenin subunits (lmw-gs) genes, the major components for glutenin subunits in hexaploid wheat. this problem, however, can be greatly simplified by characterizing the lmw-gs genes in triticum urartu, the a-genome donor of hexaploid whe ...201525849991
expression and characterization of protein disulfide isomerase family proteins in bread wheat.the major wheat seed proteins are storage proteins that are synthesized in the rough endoplasmic reticulum (er) of starchy endosperm cells. many of these proteins have intra- and intermolecular disulfide bonds. in eukaryotes, the formation of most intramolecular disulfide bonds in the er is thought to be catalyzed by protein disulfide isomerase (pdi) family proteins. the cdnas that encode eight groups of bread wheat (triticum aestivum l.) pdi family proteins have been cloned, and their expressio ...201525849633
multi-location wheat stripe rust qtl analysis: genetic background and epistatic interactions.epistasis and genetic background were important influences on expression of stripe rust resistance in two wheat ril populations, one with resistance conditioned by two major genes and the other conditioned by several minor qtl. stripe rust is a foliar disease of wheat (triticum aestivum l.) caused by the air-borne fungus puccinia striiformis f. sp. tritici and is present in most regions around the world where commercial wheat is grown. breeding for durable resistance to stripe rust continues to ...201525847212
spatial and temporal dynamics of stink bugs in southeastern farmscapes.a 3-yr study (2009-2011) was conducted to examine the spatial and temporal dynamics of stink bugs in three commercial farmscapes. study locations were replicated in south carolina and georgia, in an agriculturally diverse region known as the southeastern coastal plain. crops included wheat, triticum aestivum (l.), corn, zea mays (l.), soybean, glycine max (l.), cotton, gossypium hirsutum (l.), and peanut, arachis hypogaea (l.). farmscapes were sampled weekly using whole-plant examinations for co ...201525843577
proteome evolution of wheat (triticum aestivum l.) aleurone layer at fifteen stages of grain development.the aleurone layer (al) is the grain peripheral tissue; it is rich in micronutrients, vitamins, antioxidants, and essential amino acids. this highly nutritive part of the grain has been less studied partly because its isolation is so laborious. in the present study, the als of triticum aestivum (variety récital) were separated manually at 15 stages of grain development. a total of 327 proteins were identified using 2-de lc-ms/ms. they were classified in six main groups and 26 sub-groups accordin ...201525841591
variation in susceptibility to wheat dwarf virus among wild and domesticated wheat.we investigated the variation in plant response in host-pathogen interactions between wild (aegilops spp., triticum spp.) and domesticated wheat (triticum spp.) and wheat dwarf virus (wdv). the distribution of wdv and its wild host species overlaps in western asia in the fertile crescent, suggesting a coevolutionary relationship. bread wheat originates from a natural hybridization between wild emmer wheat (carrying the a and b genomes) and the wild d genome donor aegilops tauschii, followed by p ...201525837893
changes in genome-wide gene expression during allopolyploidization and genome stabilization in hexaploid wheat.allopolyploidization is an important evolutionary event in plants, but its genome-wide effects are not fully understood. common wheat, triticum aestivum (aabbdd), evolved through amphidiploidization between t. turgidum (aabb) and aegilops tauschii (dd). here, global gene expression patterns in the seedlings of a synthetic triploid wheat line (abd), its chromosome-doubled hexaploid (aabbdd) and stable synthetic hexaploid (aabbdd), and the parental lines t. turgidum (aabb) and ae. tauschii (dd) we ...201425832748
dissection of barley chromosomes 1h and 6h by the gametocidal system.we dissected barley chromosomes 1h and 6h added to common wheat by the gametocidal system and identified structural changes of the chromosomes by fluorescence in situ hybridization and genomic in situ hybridization. we found five aberrations of chromosome 1h, all of which lacked the long arm: one small fragment with the subtelomeric hvt01 sequence, one terminal deletion, and three telocentric chromosomes of the short arm. we established 33 dissection lines carrying single aberrant 6h chromosomes ...201425832747
genetic effect of the aegilops caudata plasmon on the manifestation of the ae. cylindrica genome.in the course of reconstructing aegilops caudata from its own genome (cc) and its plasmon, which had passed half a century in common wheat (genome aabbdd), we produced alloplasmic ae. cylindrica (genome ccdd) with the plasmon of ae. caudata. this line, designated (caudata)-ccdd, was found to express male sterility in its second substitution backcross generation (sb2) of (caudata)-aabbccdd pollinated three times with the ae. cylindrica pollen. we repeatedly backcrossed these sb2 plants with the a ...201425832746
herbal and plant therapy in patients with inflammatory bowel disease.the use of herbal therapy in inflammatory bowel disease (ibd) is increasing worldwide. the aim of this study was to review the literature on the efficacy of herbal therapy in ibd patients. studies on herbal therapy for ibd published in medline and embase were reviewed, and response to treatment and remission rates were recorded. although the number of the relevant clinical studies is relatively small, it can be assumed that the efficacy of herbal therapies in ibd is promising. the most important ...201725830661
effect of 7h(ch) hordeum chilense chromosome introgressions on the wheat endosperm proteomic profile.hordeum chilense is an excellent genetic resource for wheat breeding due to its potential to improve breadmaking quality and nutritional value and provide resistance to some biotic and abiotic stresses. hexaploid wheat lines carrying chromosome 7h(ch) introgressions, namely, chromosome additions of the whole chromosome 7h(ch) or the 7h(ch)α or the 7h(ch)β chromosome arms, and chromosome substitutions of the homeologous chromosomes 7a, 7b, or 7d by chromosome 7h(ch) were compared by 2d-page analy ...201525824108
analysis of quality-related parameters in mature kernels of polygalacturonase inhibiting protein (pgip) transgenic bread wheat infected with fusarium graminearum.fusarium head blight, caused by the fungus fusarium graminearum, has a detrimental effect on both productivity and qualitative properties of wheat. to evaluate its impact on wheat flour, we compared its effect on quality-related parameters between a transgenic bread wheat line expressing a bean polygalacturonase inhibiting protein (pgip) and its control line. we have compared metabolic proteins, the amounts of gluten proteins and their relative ratios, starch content, yield, extent of pathogen c ...201525823882
variance components, heritability and correlation analysis of anther and ovary size during the floral development of bread wheat.anther and ovary development play an important role in grain setting, a crucial factor determining wheat (triticum aestivum l.) yield. one aim of this study was to determine the heritability of anther and ovary size at different positions within a spikelet at seven floral developmental stages and conduct a variance components analysis. relationships between anther and ovary size and other traits were also assessed. the thirty central european winter wheat genotypes used in this study were based ...201525821074
effect of suppression of arabinoxylan synthetic genes in wheat endosperm on chain length of arabinoxylan and extract viscosity.arabinoxylan (ax) is the dominant component within wheat (triticum aestivum l.) endosperm cell walls, accounting for 70% of the polysaccharide. the viscosity of aqueous extracts from wheat grain is a key trait influencing the processing for various end uses, and this is largely determined by the properties of endosperm ax. we have previously shown dramatic effects on endosperm ax in transgenic wheat by down-regulating either tagt43_2 or tagt47_2 genes (orthologues to irx9 and irx10 in arabidopsi ...201625819752
development of triticum aestivum-leymus mollis translocation lines and identification of resistance to stripe rust. 201525819090
the barley uniculme4 gene encodes a blade-on-petiole-like protein that controls tillering and leaf patterning.tillers are vegetative branches that develop from axillary buds located in the leaf axils at the base of many grasses. genetic manipulation of tillering is a major objective in breeding for improved cereal yields and competition with weeds. despite this, very little is known about the molecular genetic bases of tiller development in important triticeae crops such as barley (hordeum vulgare) and wheat (triticum aestivum). recessive mutations at the barley uniculme4 (cul4) locus cause reduced till ...201525818702
(1)h-nmr screening for the high-throughput determination of genotype and environmental effects on the content of asparagine in wheat grain.free asparagine in cereals is known to be the precursor of acrylamide, a neurotoxic and carcinogenic product formed during cooking processes. thus, the development of crops with lower asparagine is of considerable interest to growers and the food industry. in this study, we describe the development and application of a rapid (1)h-nmr-based analysis of cereal flour, that is, suitable for quantifying asparagine levels, and hence acrylamide-forming potential, across large numbers of samples. the sc ...025816894
[signal function of cytokinin 6-benzylaminopurine in the reaction of triticum aestivum l. mesophyll cells to hyperthermia].the signaling effect of 6-benzylaminopurine (bap) on leaf mesophyll cells of triticum aestivum l. under hyperthermic conditions was studied. it was found that bap regulated photosynthetic pigment, hydrogen peroxide content and activity of antioxidant enzymes, namely superoxide dismutase, ascorbate peroxidase and catalase under high-temperature conditions. the additive effect of bap and high temperature on the activation of cell antioxidant systems was demonstrated. bap regulated reducing process ...201625816607
draft genome sequence of the phenazine-producing pseudomonas fluorescens strain 2-79.pseudomonas fluorescens strain 2-79, a natural isolate of the rhizosphere of wheat (triticum aestivum l.), possesses antagonistic potential toward several fungal pathogens. we report the draft genome sequence of strain 2-79, which comprises 5,674 protein-coding sequences.201525814594
in silico system analysis of physiological traits determining grain yield and protein concentration for wheat as influenced by climate and crop management.genetic improvement of grain yield (gy) and grain protein concentration (gpc) is impeded by large genotype×environment×management interactions and by compensatory effects between traits. here global uncertainty and sensitivity analyses of the process-based wheat model siriusquality2 were conducted with the aim of identifying candidate traits to increase gy and gpc. three contrasted european sites were selected and simulations were performed using long-term weather data and two nitrogen (n) treat ...201525810069
thinopyrum ponticum chromatin-integrated wheat genome shows salt-tolerance at germination stage.a wild wheatgrass, thinopyrum ponticum (2n = 10x = 70), which exhibits substantially higher levels of salt tolerance than cultivated wheat, was employed to transfer its salt tolerance to common wheat by means of wide hybridization. a highly salt-tolerant wheat line s148 (2n = 42) was obtained from the bc3f2 progenies between triticum aestivum (2n = 42) and th. ponticum. in the cross of s148 × salt-sensitive wheat variety chinese spring, the bc4f2 seeds at germination stage segregated into a rati ...201525809604
genetic and epigenetic modifications to the bbaa component of common wheat during its evolutionary history at the hexaploid level.the formation and evolution of common wheat (triticum aestivum l., genome bbaadd) involves allopolyploidization events at two ploidy levels. whether the two ploidy levels (tetraploidy and hexaploidy) have impacted the bbaa subgenomes differentially remains largely unknown. we have reported recently that extensive and distinct modifications of transcriptome expression occurred to the bbaa component of common wheat relative to the evolution of gene expression at the tetraploid level in triticum tu ...201525809554
correction: the dynamic process of interspecific interactions of competitive nitrogen capture between intercropped wheat (triticum aestivum l.) and faba bean (vicia faba l.). 201525803278
post-flowering nitrate uptake in wheat is controlled by n status at flowering, with a putative major role of root nitrate transporter nrt2.1.in bread wheat (triticum aestivum l.), the simultaneous improvement of both yield and grain protein is difficult because of the strong negative relationship between these two traits. however, some genotypes deviate positively from this relationship and this has been linked to their ability to take up nitrogen (n) during the post-flowering period, regardless of their n status at flowering. the physiological and genetic determinants of post-flowering n uptake relating to n satiety are poorly under ...201525798624
label free targeted detection and quantification of celiac disease immunogenic epitopes by mass spectrometry.celiac disease (cd) is a food-related disease caused by certain gluten peptides containing t-cell stimulating epitopes from wheat, rye, and barley. cd-patients have to maintain a gluten-free diet and are therefore dependent on reliable testing and labeling of gluten-free products. so far, the r5-elisa is the approved method to detect if food products can be labeled gluten-free. because the r5-elisa detects gluten in general, there is a demand for an improved detection method that quantifies spec ...201525795397
proteomics, peptidomics, and immunogenic potential of wheat beer (weissbier).wheat beer is a traditional light-colored top-fermenting beer brewed with at least 50% malted (e.g., german weissbier) or unmalted (e.g., belgian witbier) wheat (triticum aestivum) as an adjunct to barley (hordeum vulgare) malt. for the first time, we explored the proteome of three weissbier samples, using both 2d electrophoresis (2de)-based and 2de-free strategies. overall, 58 different gene products arising from barley, wheat, and yeast (saccharomyces spp.) were identified in the protein fract ...201525793656
antioxidative and anticarcinogenic activities of methylpheophorbide a, isolated from wheat grass (triticum aestivum linn.).methylphophorbide a (mpa) has been isolated from the ethanol extract of the wheat grass plant. its antioxidative efficacy is evaluated by hydroxyl radical scavenging activities and reducing capacity which are significantly up regulated in comparison with aqueous extract of the plant. the compound shows iron-binding capacity where the fe(2+) binds with mpa by two types of binding patterns with dissociation constants 157.17 and 27.89. it has antioxidative and cytotoxic effects on hela and hep g2 c ...201625782530
spontaneous and divergent hexaploid triticales derived from common wheat × rye by complete elimination of d-genome chromosomes.hexaploid triticale could be either synthesized by crossing tetraploid wheat with rye, or developed by crossing hexaploid wheat with a hexaploid triticale or an octoploid triticale.201525781330
tanac1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to pseudomonas syringae, and promotes lateral root development in transgenic arabidopsis thaliana.plant-specific nac transcription factors (tfs) constitute a large family and play important roles in regulating plant developmental processes and responses to environmental stresses, but only some of them have been investigated for effects on disease reaction in cereal crops. virus-induced gene silencing (vigs) is an effective strategy for rapid functional analysis of genes in plant tissues. in this study, tanac1, encoding a new member of the nac1 subgroup, was cloned from bread wheat and charac ...201525774162
sharing a host plant (wheat [triticum aestivum]) increases the fitness of fusarium graminearum and the severity of fusarium head blight but reduces the fitness of grain aphids (sitobion avenae).we hypothesized that interactions between fusarium head blight-causing pathogens and herbivores are likely to occur because they share wheat as a host plant. our aim was to investigate the interactions between the grain aphid, sitobion avenae, and fusarium graminearum on wheat ears and the role that host volatile chemicals play in mediating interactions. wheat ears were treated with aphids and f. graminearum inoculum, together or separately, and disease progress was monitored by visual assessmen ...201525769834
genome-wide characterization of developmental stage- and tissue-specific transcription factors in wheat.wheat (triticum aestivum) is one of the most important cereal crops, providing food for humans and feed for other animals. however, its productivity is challenged by various biotic and abiotic stresses such as fungal diseases, insects, drought, salinity, and cold. transcription factors (tfs) regulate gene expression in different tissues and at various developmental stages in plants and animals, and they can be identified and classified into families according to their structural and specialized ...025766308
precisely mapping a major gene conferring resistance to hessian fly in bread wheat using genotyping-by-sequencing.one of the reasons hard red winter wheat cultivar 'duster' (pi 644016) is widely grown in the southern great plains is that it confers a consistently high level of resistance to biotype gp of hessian fly (hf). however, little is known about the genetic mechanism underlying hf resistance in duster. this study aimed to unravel complex structures of the hf region on chromosome 1as in wheat by using genotyping-by-sequencing (gbs) markers and single nucleotide polymorphism (snp) markers.201525765046
adventitious roots of wheat seedlings that emerge in oxygen-deficient conditions have increased root diameters with highly developed lysigenous aerenchyma.exposing roots of plants to hypoxic conditions is known to greatly improve their anoxic stress tolerance. we previously showed that pre-treatment of wheat seedlings with an ethylene precursor, 1-aminocyclopropanecarboxylic acid (acc), enhanced their tolerance of oxygen-deficient conditions. although acc-pretreated seminal roots of wheat seedlings grown under oxygen-deficient conditions avoided root tip death, they elongated very little. in the present study, we assessed the effects of ethylene o ...201425764431
allergen relative abundance in several wheat varieties as revealed via a targeted quantitative approach using ms.food allergy has become a major health issue in developed countries, therefore there is an urgent need to develop analytical methods able to detect and quantify with a good sensitivity and reliability some specific allergens in complex food matrices. in this paper, we present a targeted ms/ms approach to compare the relative abundance of the major recognized wheat allergens in the salt-soluble (albumin/globulin) fraction of wheat grains. twelve allergens were quantified in seven wheat varieties, ...201525764008
resistance of callose synthase activity to free fatty acid inhibition as an indicator of fusarium head blight resistance in wheat.the fungal pathogen fusarium graminearum is the causal agent of fusarium head blight (fhb); a devastating crop disease resulting in heavy yield losses and grain contamination with mycotoxins. we recently showed that the secreted lipase fgl1, a virulence factor of f. graminearum, targets plant defense-related callose biosynthesis during wheat head infection. this effector-like function is based on a fgl1-mediated release of polyunsaturated free fatty acids (ffa) that can inhibit callose synthase ...201425763484
arabidopsis ef-tu receptor enhances bacterial disease resistance in transgenic wheat.perception of pathogen (or microbe)-associated molecular patterns (pamps/mamps) by pattern recognition receptors (prrs) is a key component of plant innate immunity. the arabidopsis prr ef-tu receptor (efr) recognizes the bacterial pamp elongation factor tu (ef-tu) and its derived peptide elf18. previous work revealed that transgenic expression of atefr in solanaceae confers elf18 responsiveness and broad-spectrum bacterial disease resistance. in this study, we developed a set of bioassays to stu ...201525760815
chromosome constitution and origin analysis in three derivatives of triticum aestivum--leymus mollis by molecular cytogenetic identification.leymus mollis (2n = 4x = 28, nsnsxmxm) is an important tetraploid species in leymus (poaceae: triticeae) and a useful genetic resource for wheat breeding because of the stress tolerance and disease resistance of this species. the development of triticum aestivum (common wheat) - l. mollis derivatives with desirable genes will provide valuable bridge materials for wheat improvement, especially regarding powdery mildew resistance genes, which are rarely documented in l. mollis. in the present stud ...201425760775
plant growth promotion and root colonization by eps producing enterobacter sp. rzs5 under heavy metal contaminated soil.the heavy metal resistant bacterium isolated from field soil and identified as enterobacter sp. rzs5 tolerates a high concentration (100-2000 μm) of various heavy metal ions such as mn2+, ni2+, zn2+, cu2+, co2+ and fe2+ when grown in such environment and produces exopolysaccharides (eps). here, we have demonstrated eps production by enterobacter sp. rzs5 during 60 h of growth in yeast extract mannitol broth (yemb). the yield increased by two fold after the addition of 60 μm of ca2+; 50 μm of fe2 ...201525757243
responses of arabidopsis and wheat to rising co2 depend on nitrogen source and nighttime co2 levels.a major contributor to the global carbon cycle is plant respiration. elevated atmospheric co2 concentrations may either accelerate or decelerate plant respiration for reasons that have been uncertain. we recently established that elevated co2 during the daytime decreases plant mitochondrial respiration in the light and protein concentration because co2 slows the daytime conversion of nitrate (no3 (-)) into protein. this derives in part from the inhibitory effect of co2 on photorespiration and th ...201525755253
genome-wide association mapping for stripe rust (puccinia striiformis f. sp. tritici) in us pacific northwest winter wheat (triticum aestivum l.).potential novel and known qtl for race-specific all-stage and adult plant resistance to stripe rust were identified by genome-wide association mapping in the us pnw winter wheat accessions. stripe rust (puccinia striiformis f. sp. tritici; also known as yellow rust) is a globally devastating disease of wheat (triticum aestivum l.) and a major threat to wheat production in the us pacific northwest (pnw), therefore both adult plant and all-stage resistance have been introduced into the winter whea ...201525754424
responses of wheat (triticum aestivum) and turnip (brassica rapa) to the combined exposure of carbaryl and ultraviolet radiation.the increase of ultraviolet (uv) radiation reaching the earth's surface as a result of increased ozone layer depletion has affected crop production systems and, in combination with pesticides used in agricultural activities, can lead to greater risks to the environment. the impact of uv radiation and carbaryl singly and in combination on triticum aestivum (wheat) and brassica rapa (turnip) was studied. the combined exposure was analyzed using the mixtox tool and was based on the conceptual model ...201525754078
evaluation of phytotoxicity and ecotoxicity potentials of a cyanobacterial extract containing microcystins under realistic environmental concentrations and in a soil-plant system.the impact of a crude extract of microcystis aeruginosa (pcc7820) containing 14 microcystin variants was investigated on seeds germination and radicles development of four agricultural plants: two tomato varieties solanum lycopersicum (microtom and saint-pierre), the wheat triticum aestivum and the lettuce lactuca sativa. in addition, the effect of 14 d-exposure to irrigation water containing realistic concentrations of microcystins (0-0.1 mg eq. microcystin-lrl(-1)) on the tomato microtom seedl ...201525754013
alleviation of chromium toxicity by glycinebetaine is related to elevated antioxidant enzymes and suppressed chromium uptake and oxidative stress in wheat (triticum aestivum l.).little information is available on the role of glycinebetaine (gb) in chromium (cr) tolerance while cr toxicity is widespread problem in crops grown on cr-contaminated soils. in this study, we investigated the influence of gb on cr tolerance in wheat (triticum aestivum l.) grown in sand and soil mediums. three concentrations of chromium (0, 0.25, and 0.5 mm) were tested with and without foliar application of gb (0.1 m). chromium alone led to a significant growth inhibition and content of chlorop ...201525752628
roles of dehydrin genes in wheat tolerance to drought stress.physiological parameters and expression levels of drought related genes were analyzed in early vegetative stage of two bread wheat cultivars (sids and gmiza) differ in drought tolerance capacity. both cultivars were imposed to gradual water depletion started on day 17 till day 32 after sowing. sids, the more tolerant cultivar to drought showed higher fresh and dry weights than the drought sensitive genotype, gmiza. under water stress, sids had higher membrane stability index (msi), lower accumul ...201525750752
high-throughput phenotyping of seminal root traits in wheat.water availability is a major limiting factor for wheat (triticum aestivum l.) production in rain-fed agricultural systems worldwide. root system architecture has important functional implications for the timing and extent of soil water extraction, yet selection for root architectural traits in breeding programs has been limited by a lack of suitable phenotyping methods. the aim of this research was to develop low-cost high-throughput phenotyping methods to facilitate selection for desirable roo ...201525750658
linking salinity stress tolerance with tissue-specific na(+) sequestration in wheat roots.salinity stress tolerance is a physiologically complex trait that is conferred by the large array of interacting mechanisms. among these, vacuolar na(+) sequestration has always been considered as one of the key components differentiating between sensitive and tolerant species and genotypes. however, vacuolar na(+) sequestration has been rarely considered in the context of the tissue-specific expression and regulation of appropriate transporters contributing to na(+) removal from the cytosol. in ...201525750644
root length densities of uk wheat and oilseed rape crops with implications for water capture and yield.root length density (rld) was measured to 1 m depth for 17 commercial crops of winter wheat (triticum aestivum) and 40 crops of winter oilseed rape [brassica napus; oilseed rape (osr)] grown in the uk between 2004 and 2013. taking the critical rld (crld) for water capture as 1cm cm(-3), rlds appeared inadequate for full water capture on average below a depth of 0.32 m for winter wheat and below 0.45 m for osr. these depths compare unfavourably (for wheat) with average depths of 'full capture' of ...201525750427
making the bread: insights from newly synthesized allohexaploid wheat.bread wheat (or common wheat, triticum aestivum) is an allohexaploid (aabbdd, 2n = 6x = 42) that arose by hybridization between a cultivated tetraploid wheat t. turgidum (aabb, 2n = 4x = 28) and the wild goatgrass aegilops tauschii (dd, 2n = 2x = 14). polyploidization provided niches for rigorous genome modification at cytogenetic, genetic, and epigenetic levels, rendering a broader spread than its progenitors. this review summarizes the latest advances in understanding gene regulation mechanism ...201525747845
fulvic acid mediates chromium (cr) tolerance in wheat (triticum aestivum l.) through lowering of cr uptake and improved antioxidant defense system.chromium (cr) stress is one of the most adverse environmental factors that affect plant growth and food chain contamination. fulvic acid (fa) is known to enhance the growth and production of crops, but the studies are scare regarding the application of fa on metal tolerance in plants. the effects of fa application on alleviating cr phytotoxicity in wheat plants were investigated in a pot experiment conducted in sand- and soil-grown plants. three cr (0, 0.25, and 0.50 mm) treatments in the form o ...201525744818
phenotyping pipeline reveals major seedling root growth qtl in hexaploid wheat.seedling root traits of wheat (triticum aestivum l.) have been shown to be important for efficient establishment and linked to mature plant traits such as height and yield. a root phenotyping pipeline, consisting of a germination paper-based screen combined with image segmentation and analysis software, was developed and used to characterize seedling traits in 94 doubled haploid progeny derived from a cross between the winter wheat cultivars rialto and savannah. field experiments were conducted ...201525740921
identification of new ssr markers linked to leaf chlorophyll content, flag leaf senescence and cell membrane stability traits in wheat under water stressed condition.segregating f4 families from the cross between drought sensitive (yecora rojo) and drought tolerant (pavon 76) genotypes were made to identify ssr markers linked to leaf chlorophyll content, flag leaf senescence and cell membrane stability traits in wheat (triticum aestivum l.) under water-stressed condition and to map quantitative trait locus (qtl) for the three physiological traits. the parents and 150 f4 families were evaluated phenotypically for drought tolerance using two irrigation treatme ...201525740441
localization of ben1-like protein and nuclear degradation during development of metaphloem sieve elements in triticum aestivum l.metaphloem sieve elements (mses) in the developing caryopsis of triticum aestivum l. undergo a unique type of programmed cell death (pcd); cell organelles gradually degrade with the mse differentiation while mature sieve elements keep active. this study focuses on locating ben1-like protein and nuclear degradation in differentiating mses of wheat. transmission electron microscopy (tem) showed that nuclei degraded in mse development. first, the degradation started at 2-3 days after flowering (daf ...201525740439
[investigation of morphogenesis of inflorescence and determination of the nature of inheritance of "supernumerary spikelets" trait of bread wheat (triticum aestivum l.) mutant line].using c-banding and fish methods, the karyotype of mc1611 induced mutant of bread wheat, which develop additional spikelets at a rachis node (trait "supernumerary spikelets") was characterized. it was determined that the mutant phenotype is not associated with aneuploidy and major chromosomal rearrangements. the results of genetic analysis showed that supernumerary spikelets of the line are caused by a mutation of the single bh-d. 1 gene, influenced by the genetic background. the mutation causes ...201525739301
[chromosome composition of wheat-rye lines and the influence of rye chromosomes on disease resistance and agronomic traits].identification of the chromosomal composition of common wheat lines with rye chromosomes was carried out using genomic in situ hybridization and 1rs- and 5p-specific pcr markers. it was demonstrated that wheat chromosomes 5a or 5d were substituted by rye chromosome 5r in the wheat-rye lines. it was established that one of the lines with complex disease resistance contained rye chromosome 5r and t1rs.1bl, while another line was found to contain, in addition to t1rs.1bl, a new robertsonian translo ...201425739285
significance and value of non-traded ecosystem services on farmland.background. ecosystem services (es) generated within agricultural landscapes, including field boundaries, are vital for the sustainable supply of food and fibre. however, the value of es in agriculture has not been quantified experimentally and then extrapolated globally. methods. we quantified the economic value of two key but contrasting es (biological control of pests and nitrogen mineralisation) provided by non-traded non-crop species in ten organic and ten conventional arable fields in new ...201525737811
host-induced gene silencing of an essential chitin synthase gene confers durable resistance to fusarium head blight and seedling blight in wheat.fusarium head blight (fhb) and fusarium seedling blight (fsb) of wheat, caused by fusarium pathogens, are devastating diseases worldwide. we report the expression of rna interference (rnai) sequences derived from an essential fusarium graminearum (fg) virulence gene, chitin synthase (chs) 3b, as a method to enhance resistance of wheat plants to fungal pathogens. deletion of chs3b was lethal to fg; disruption of the other chs gene family members generated knockout mutants with diverse impacts on ...201525735638
[mitotic behavior of centromeres in meiosis as the fertility restoration mechanism in wheat-rye amphihaploids].the regulation of chromosomal behavior in meiosis in partly fertile wheat-rye amphihaploids was studied using the centromere specific probes pawrc1 and ae. tauschii paet6-09. comparative analysis of the probe localization patterns in mitosis, normal meiosis in wheat triticum aestivum l. and rye secale cereale l., and meiosis in amphihaploids was performed. the differences in the structure of centromeres in monopolar- and bipolar- oriented chromosomes were revealed. single dense hybridization sig ...201425731022
relationship between male sterility and β-1,3-glucanase activity and callose deposition-related gene expression in wheat (triticum aestivum l.).in previous studies, we first isolated one different protein β-1,3-glucanase using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry from normal wheat (triticum aestivum l.) and chemical hybridization agent-induced male sterility (cims) wheat. in this experiment, β-1,3-glucanase activity and the expression of a callose deposition-related gene, udp-glucose phosphorylase (ugpase), were determinate in normal, cims, and genetic male ster ...201525729993
[the role of reactive oxygen species and calcium ions in the implementation of the stress-protective effect of brassinosteroids on plant cells].the effect of the brassinosteroids (bs) 24-epibrassiniolide and 24-epicastasterone on the thermoresistance of wheat coleoptile (triticum aestivum l.) and their generation of the superoxide anion radical and antioxidant enzymes activity were investigated. the treatment of coleoptiles with 10 nm solutions of bs caused a transient increase in o2·- generation and a subsequent increase in the activity of superoxide dismutase and catalase and an improvement in heat resistance. pretreatment of coleopti ...201725726668
chromosome engineering, mapping, and transferring of resistance to fusarium head blight disease from elymus tsukushiensis into wheat.this manuscript describes the transfer and molecular cytogenetic characterization of a novel source of fusarium head blight resistance in wheat. fusarium head blight (fhb) caused by the fungus fusarium graminearum schwabe [telomorph = gibberella zeae (schwein. fr.) petch] is an important disease of bread wheat, triticum aestivum l. (2n = 6x = 42, aabbdd) worldwide. wheat has limited resistance to fhb controlled by many loci and new sources of resistance are urgently needed. the perennial grass e ...201525726000
[nuclear-cytoplasmic compatibility and the state of mitochondrial and chloroplast dna regions in alloplasmic recombinant and introgressive lines (h. vulgare)-t. aestivum].alloplasmic lines combining alien nuclear and cytoplasmic genomes are convenient models for studying the mechanisms of nuclear-cytoplasmic compatibility/incompatibility. in the.present study, we have investigated the correlation between the characters and state of mitochondrial (mt) and chloroplast (cp) dna regions in alloplasmic recombinant common wheat lines with barley cytoplasm characterized by partial or total fertility. fertility restoration in the studied lines (hordeum vulgare)-triticum ...201425720248
[the influence of combinations of alien translocations on in vitro androgenesis in near-isogenic lines of spring bread wheat].the features of in vitro androgenesis were studied in cultured anthers of spring bread wheats l503 and dobrynya, having 7ds-7dl-7ae#1 l translocation with genes lrl9/sr25 (lrl9 translocation) from agropyron elongatum (host.) p.b. and their near-isogenic lines carrying combinations of lrl9 translocation with translocations: 1bl-ir#1s with genes pm8/sr31/lr26/yr9 (lr26translocation) from secale cereal l., 4bs-4bl-2r#1l with genes lr25/pm7 (lr25 translocation) from secale cereal, 3ds-3dl-3ae#1l wit ...201425720141
[characteristics of alpha-amylase isozymes in cytologenetically different wheat cultivars].the isoenzyme composition of alpha-amylase is studied by polyacrylamide gel electrophoresis in tris-glycine (ph 8.3) system in wheat cultivars with different genome composition. we show that durum wheat (triticum durum, 2n=4x=28, bbaa) lacks the isoenzymes encoded by 6d and 7d chromosomes that are present in common wheat zymograms (triticum aestivum, 2n=6x=42, bbaadd). a similar pattern is observed in a synthetic allohexaploid carrying the bbaa genomes of wheat and the hchhch genome of barley (h ...201425720140
[detection of genetic determinants that define the difference of near-isogenic triticum aestivum l. lines in photoperiodic sensitivity].identification of genetic determinants that define different degrees of line sensitivity to the photoperiod was conducted on material of near-isogenic lines of the soft hexaploid wheat triticum aestivum l. using ssr markers and markers specific to the vrn and ppd genes. it was established that the ppd-s line contains a dominant ppd-dla allele located on chromosome 2d. this allele is characterized by a vast deletion in the gene promoter region. for two other lines (ppd-m and ppd-w), introgression ...201425720138
transmission of escherichia coli o157:h7 to internal tissues and its survival on flowering heads of wheat.escherichia coli o157:h7 is a human pathogen that can cause bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome. e. coli o157:h7 illnesses are mainly associated with undercooked beef; however, in recent years, outbreaks have been linked to fresh produce, such as spinach, lettuce, and sprouts. in 2009, flour was implicated as the contamination source in an outbreak involving consumption of raw cookie dough that resulted in 77 illnesses. the objectives of this research were to dete ...201525719875
natural colloidal p and its contribution to plant p uptake.phosphorus (p) bioavailability depends on its concentration and speciation in solution. andisols and oxisols have very low soil solution concentration of free orthophosphate, as they contain high concentrations of strongly p-sorbing minerals (al/fe oxyhydroxides, allophanes). free orthophosphate is the form of p taken up by plants, but it is not the only p species present in the soil solution. natural colloidal p (p associated with al, fe, and organic matter of sizes ranging from 1 to 1000 nm) c ...201525719745
an assessment of heavy ion irradiation mutagenesis for reverse genetics in wheat (triticum aestivum l.).reverse genetic techniques harnessing mutational approaches are powerful tools that can provide substantial insight into gene function in plants. however, as compared to diploid species, reverse genetic analyses in polyploid plants such as bread wheat can present substantial challenges associated with high levels of sequence and functional similarity amongst homoeologous loci. we previously developed a high-throughput method to identify deletions of genes within a physically mutagenized wheat po ...201525719507
evaluation of wheat chromosome translocation lines for high temperature stress tolerance at grain filling stage.high temperature (ht, heat) stress is detrimental to wheat (triticum aestivum l.) production. wild relatives of bread wheat may offer sources of ht stress tolerance genes because they grow in stressed habitats. wheat chromosome translocation lines, produced by introgressing small segments of chromosome from wild relatives to bread wheat, were evaluated for tolerance to ht stress during the grain filling stage. sixteen translocation lines and four wheat cultivars were grown at optimum temperature ...201525719199
addition of rye chromosome 4r to wheat increases anther length and pollen grain number.the research identified rye chromosome 4r arms associated with good pollinator traits, and demonstrated possible use of rye genetic resources to develop elite pollinators for hybrid wheat breeding. bread wheat (triticum aestivum) is a predominantly self-pollinating plant which has relatively small-sized anthers and produces a low number of pollen grains. these features limit the suitability of most wheat lines as pollinators for hybrid seed production. one strategy for improving the pollination ...201525716820
relationships between wheat grain physical characteristics studied through near-isogenic lines with distinct puroindoline-b allele.genetic (different forms of puroindoline-b) and environment (through variations in vitreousness), have important effects on wheat grain mechanical properties. the two methods of hardness measurements (nirs, skcs) do not give the same information. bread wheat near-isogenic lines differing in hardness, due to distinct puroindoline-b alleles (the wild type, pinb-d1a, or the mutated forms, pinb-d1b or pinb-d1d), were grown for three years in seven sites and under two nitrogen fertilization levels, t ...201525716819
isolation and characterization of rubisco small subunit gene promoter from common wheat (triticum aestivum l.).choice of an appropriate promoter is critical to express target genes in intended tissues and developmental stages. however, promoters capable of directing gene expression in specific tissues and stages are not well characterized in monocot species. to identify such a promoter in wheat, this study isolated a partial sequence of the wheat small subunit of rubisco (tarbcs) promoter. in silico analysis revealed the presence of elements that are characteristic to rbcs promoters of other, mainly dico ...201525713931
priming of wheat with the green leaf volatile z-3-hexenyl acetate enhances defense against fusarium graminearum but boosts deoxynivalenol production.priming refers to a mechanism whereby plants are sensitized to respond faster and/or more strongly to future pathogen attack. here, we demonstrate that preexposure to the green leaf volatile z-3-hexenyl acetate (z-3-hac) primed wheat (triticum aestivum) for enhanced defense against subsequent infection with the hemibiotrophic fungus fusarium graminearum. bioassays showed that, after priming with z-3-hac, wheat ears accumulated up to 40% fewer necrotic spikelets. furthermore, leaves of seedlings ...201525713338
confocal laser scanning microscopy elucidation of the micromorphology of the leaf cuticle and analysis of its chemical composition.electron microscopy techniques such as transmission electron microscopy (tem) and scanning electron microscopy (sem) have been invaluable tools for the study of the micromorphology of plant cuticles. however, for electron microscopy, the preparation techniques required may invariably introduce artefacts in cuticle preservation. further, there are a limited number of methods available for quantifying the image data obtained through electron microscopy. therefore, in this study, optical microscopy ...201525712592
diversity trends in bread wheat in italy during the 20th century assessed by traditional and multivariate approaches.a collection of 157 triticum aestivum accessions, representative of wheat breeding in italy during the 20(th) century, was assembled to describe the evolutionary trends of cultivated varieties throughout this period. the lines were cultivated in italy, in two locations, over two growing seasons, and evaluated for several agronomical, morphological and qualitative traits. analyses were conducted using the most common univariate approach on individual plant traits coupled with a correspondance mul ...201525712271
new insights into the wheat chromosome 4d structure and virtual gene order, revealed by survey pyrosequencing.survey sequencing of the bread wheat (triticum aestivum l.) genome (aabbdd) has been approached through different strategies delivering important information. however, the current wheat sequence knowledge is not complete. the aim of our study is to provide different and complementary set of data for chromosome 4d. a survey sequence was obtained by pyrosequencing of flow-sorted 4ds (7.2×) and 4dl (4.1×) arms. single ends (se) and long mate pairs (lmp) reads were assembled into contigs (223mb) and ...201525711827
detection of qtl for metabolic and agronomic traits in wheat with adjustments for variation at genetic loci that affect plant phenology.mapping of quantitative trait loci associated with levels of individual metabolites (mqtl) was combined with the mapping of agronomic traits to investigate the genetic basis of variation and co-variation in metabolites, agronomic traits, and plant phenology in a field-grown bread wheat population. metabolome analysis was performed using liquid chromatography-mass spectrometry resulting in identification of mainly polar compounds, including secondary metabolites. a total of 558 metabolic features ...201525711822
[genetic analysis of leaf pubescence in isogenic lines of conmmon wheat novosibirsk 67].in this study, genetic and monosomic analyses of the leaf pubescence of ank 7a, ank 7b, and ank 7c wheat isogenic lines were carried out based on the novosibirsk 67 wheat variety. according to visual analysis, the variety-recipient has a soft, uniform pubescence, and lines have trichomes on the surfaces of their leaves inherited from the two. chinese varieties and one soviet variety. using the high throughput phenotyping method lhdetect2, which allows one to allocate the phenotypic classes of of ...201425711025
[the effectiveness of molecular markers for the identification of lr28, lr35, and lr47 genes in common wheat].the effectiveness of molecular markers for the identification of leaf rust resistance genes lr28, lr35, lr47 transferred to common wheat was assessed the using samplesof triticum spp. and aegilops spp. from ae. speltoides. markers sr39f2/r3, bcd260f1/35r2 of gene lr35 and ps10 of lr47 gene were characterized by high efficiency and were revealed in a line of common wheat containing these genes, and samples of ae. speltoides (their donor). marker scs421 of lr28gene and markers sr39#22r, sr39#50s, ...201425711022
virus-induced gene silencing-based functional verification of six genes associated with vernalization in wheat.vernalization requirement is an important characteristic in crop breeding. wheat is a widely grown crop in the world that possesses enormous economic significance. to better understand the gene networks in vernalization process, we performed a high-throughput rna sequencing analysis comparing the transcriptomes of spring and winter wheat cultivars, with and without vernalization (unpublished data). in this study, we selected six unigenes (cl14010, cl12788, cl176, unigene 16777, cl8746 and unigen ...201525707852
common genetic basis for canopy temperature depression under heat and drought stress associated with optimized root distribution in bread wheat.qtl related to cooler canopy temperatures are associated with optimal root distribution whereby roots proliferate at depth under drought or near to surface under hot, irrigated conditions. previous research using a bread wheat ril population of the seri/babax cross showed that common qtl were associated with cooler canopies under both drought and heat-stressed conditions. a subset of ril was grown under water-limited and hot-irrigated field environments to test how cooler canopies are related to ...201525707766
[activity of protective proteins in wheat plants treated with chitooligosaccharides with different degrees of acetylation and infection with bipolaris sorokiniana].the influence of chitooligosaccharides (cos) with different degrees of acetylation (da) on the production of hydrogen peroxide (h2o2) and changes in the level of gene expression of pathogenesis-related (pr) proteins (oxalate oxidase aj556991.1, peroxidase tc 151917, chitinase av029935l, proteinase inhibitor eu293132.1) in the roots of the wheat triticum aestivum l. inoculated with root rot pathogen bipolaris sorokiniana (sacc.) shoenaker was investigated. differences were detected in plant respo ...201625707110
[the effect of 1-methylcyclopropene on the components of pro- and antioxidant systems of wheat and the development of protective reactions in fungal pathogenesis].the effect of 1-methylcyclopropene (1-mcp), which inhibits the reception of ethylene, on the following has been studied: hydrogen peroxide generation, oxalate oxidase activity, peroxidase activity, catalase activity, and lignin accumulation in infected leaves of soft spring wheat (triticum aestivum l.) breeds that differ in their resistance to the causative agent of leaf blotch septoria nodorum berk. a decrease in the development of leaf blotch in wheat leaves under the influence of 1-mcp was, o ...201625707109
colonisation of winter wheat grain by fusarium spp. and mycotoxin content as dependent on a wheat variety, crop rotation, a crop management system and weather conditions.field experiments were conducted during three consecutive growing seasons (2007/08, 2008/09 and 2009/10) with four winter wheat (triticum aestivum l.) cultivars - 'bogatka', 'kris', 'satyna' and 'tonacja' - grown on fields with a three-field crop rotation (winter triticale, spring barley, winter wheat) and in a four-field crop rotation experiment (spring wheat, spring cereals, winter rapeseed, winter wheat). after the harvest, kernels were surface disinfected with 2% naocl and then analysed for ...025705931
tarar1 and tasgt1 associate with tahsp90 to function in bread wheat (triticum aestivum l.) seedling growth and stripe rust resistance.rar1 and sgt1 are important co-chaperones of hsp90. we previously showed that tahsp90.1 is required for wheat seedling growth, and that tahsp90.2 and tahsp90.3 are essential for resistance (r) gene mediated resistance to stripe rust fungus. here, we report the characterization of tarar1 and tasgt1 genes in bread wheat. tarar1 and tasgt1 each had three homoeologs, which were located on wheat groups 2 and 3 chromosomes, respectively. strong inhibition of seedling growth was observed after silencin ...201525697954
effects of modification and incorporation techniques on disintegrant properties of wheat (triticum aestivum) starch in metronidazole tablet formulations.natural polymers serve as cheap, non-toxic, biocompatible excipients in drug delivery.201525696939
interacting agricultural pests and their effect on crop yield: application of a bayesian decision theory approach to the joint management of bromus tectorum and cephus cinctus.worldwide, the landscape homogeneity of extensive monocultures that characterizes conventional agriculture has resulted in the development of specialized and interacting multitrophic pest complexes. while integrated pest management emphasizes the need to consider the ecological context where multiple species coexist, management recommendations are often based on single-species tactics. this approach may not provide satisfactory solutions when confronted with the complex interactions occurring be ...201525692673
genetic control of plant height in european winter wheat cultivars.plant height variation in european winter wheat cultivars is mainly controlled by the rht - d1 and rht - b1 semi-dwarfing genes, but also by other medium- or small-effect qtl and potentially epistatic qtl enabling fine adjustments of plant height. plant height is an important goal in wheat (triticum aestivum l.) breeding as it affects crop performance and thus yield and quality. the aim of this study was to investigate the genetic control of plant height in european winter wheat cultivars. to th ...201525687129
silver sulfide nanoparticles (ag2s-nps) are taken up by plants and are phytotoxic.silver nanoparticles (nps) are used in more consumer products than any other nanomaterial and their release into the environment is unavoidable. of primary concern is the wastewater stream in which most silver nps are transformed to silver sulfide nps (ag2s-nps) before being applied to agricultural soils within biosolids. while ag2s-nps are assumed to be biologically inert, nothing is known of their effects on terrestrial plants. the phytotoxicity of ag and its accumulation was examined in short ...201525686712
profiling of spatial metabolite distributions in wheat leaves under normal and nitrate limiting conditions.the control and interaction between nitrogen and carbon assimilatory pathways is essential in both photosynthetic and non-photosynthetic tissue in order to support metabolic processes without compromising growth. physiological differences between the basal and mature region of wheat (triticum aestivum) primary leaves confirmed that there was a change from heterotrophic to autotrophic metabolism. fourier transform infrared (ft-ir) spectroscopy confirmed the suitability and phenotypic reproducibil ...201525680480
signal transduction during wheat grain development.this review examines the signaling pathways from the developmental and environmental point of view and the interactions among external conditions, hormonal regulations, and sugarsensing in wheat. grain development is the key phase of reproductive growth that is closely associated with vegetative organ senescence, initiation of grain filling, pre-stored assimilates remobilization, and maturation. senescence is characterized by loss of chlorophyll and the degradation of proteins, nucleic acids, li ...201525680351
fine mapping of the first multi-fertility-restoring gene, rf(multi), of wheat for three aegilops plasmons, using 1bs-1rs recombinant lines.fertility-restoring genes, rfv1, rfm1 and rfn1, respectively, for the male sterile cytoplasms of aegilops kotschyi, ae. mutica and ae. uniaristata to common wheat were located on the same locus of pavon wheat 1bs arm. the male sterile cytoplasm (plasmon) and the fertility-restoring gene are essential genetic components for breeding hybrid seed crops. this article represents information on the genetic similarity of three aegilops plasmons usable as the male sterile cytoplasm for hybrid wheat and ...201525673141
molecular mapping of a new powdery mildew resistance gene pm2b in chinese breeding line km2939.an allele of pm2 for wheat powdery mildew resistance was identified in a putative agropyron cristatum -derived line and used in wheat breeding programs. powdery mildew (caused by blumeria graminis f. sp. tritici, bgt) is one of the most devastating wheat diseases worldwide. it is important to exploit varied sources of resistance from common wheat and its relatives in resistance breeding. km2939, a chinese breeding line, exhibits high resistance to powdery mildew at both the seedling and adult st ...201525673140
genetic and epigenetic changes in somatic hybrid introgression lines between wheat and tall wheatgrass.broad phenotypic variations were induced in derivatives of an asymmetric somatic hybridization of bread wheat (triticum aestivum) and tall wheatgrass (thinopyrum ponticum podp); however, how these variations occurred was unknown. we explored the nature of these variations by cytogenetic assays and dna profiling techniques to characterize six genetically stable somatic introgression lines. karyotyping results show the six lines similar to their wheat parent, but gish analysis identified the prese ...201525670745
tricin, a flavonoid monomer in monocot lignification.tricin was recently discovered in lignin preparations from wheat (triticum aestivum) straw and subsequently in all monocot samples examined. to provide proof that tricin is involved in lignification and establish the mechanism by which it incorporates into the lignin polymer, the 4'-o-β-coupling products of tricin with the monolignols (p-coumaryl, coniferyl, and sinapyl alcohols) were synthesized along with the trimer that would result from its 4'-o-β-coupling with sinapyl alcohol and then conif ...201525667313
using the uk reference population avalon × cadenza as a platform to compare breeding strategies in elite western european bread wheat.wheat breeders select for qualitative and quantitative traits, the latter often detected as quantitative trait loci (qtl). it is, however, a long procedure from qtl discovery to the successful introduction of favourable alleles into new elite varieties and finally into farmers' crops. as a proof of principle for this process, qtl for grain yield (gy), yield components, plant height (ph), ear emergence (em), solid stem (ss) and yellow rust resistance (yr) were identified in segregating uk bread w ...201725663815
increased prediction accuracy in wheat breeding trials using a marker × environment interaction genomic selection model.genomic selection (gs) models use genome-wide genetic information to predict genetic values of candidates of selection. originally, these models were developed without considering genotype × environment interaction(g×e). several authors have proposed extensions of the single-environment gs model that accommodate g×e using either covariance functions or environmental covariates. in this study, we model g×e using a marker × environment interaction (m×e) gs model; the approach is conceptually simpl ...201525660166
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