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estimating photosynthetic electron transport via chlorophyll fluorometry without photosystem ii light saturation.estimates of thylakoid electron transport rates (j(e)) from chlorophyll fluorometry are often used in combination with leaf gas exchange measurements to provide detailed information about photosynthetic activity of leaves in situ. estimating j(e) requires accurate determination of the quantum efficiency of photosystem ii (phi(p)), which in turn requires momentary light saturation of the photosystem ii light harvesting complex to induce the maximum fluorescence signal (f(m)'). in practice, full s ...200416151873
characterization of a cdna encoding metallothionein 3 from cotton (gossypium hirsutum l.).a cdna encoding metallothionein (mt) was isolated from a library constructed with poly a(+) rna purified from 48 h etiolated cotton (gossypium hirsutum l.) cotyledons. this cdna encodes a deduced protein with 63 residues and a molecular weight of 6.3 kda. the protein has 10 cysteines of which 4 are within the cxxcxcxxxxxc amino-terminus motif and six are within the cxcxxxcxcxxcxc carboxyl-terminus motif characteristic of the type iii mt (mt3). the cotton mt3 protein sequence is 76.2, 69.8, 66.7, ...200516147860
cleaved aflp (caflp), a modified amplified fragment length polymorphism analysis for cotton.in certain plant species including cotton (gossypium hirsutum l. or gossypium barbadense l.), the level of amplified fragment length polymorphism (aflp) is relatively low, limiting its utilization in the development of genome-wide linkage maps. we propose the use of frequent restriction enzymes in combination with aflp to cleave the aflp fragments, called cleaved aflp analysis (caflp). using four upland cotton genotypes (g. hirsutum) and three pima cotton (g. barbadense), we demonstrated that ca ...200516133304
a comparison of genetic maps constructed from haploid and bc1 mapping populations from the same crossing between gossypium hirsutum l. and gossypium barbadense l.simple sequence repeat (ssr) genetic maps have been separately constructed based on doubled haploid (dh) and (or) haploid and bc1 populations from the same cross between gossypium hirsutum l. 'tm-1' and gossypium barbadense l. 'hai7124'. the bc1 population was produced by pollinating individual plants of the 'tm-1' x 'hai7124' f1 with 'tm-1', whereas the dh and (or) haploid population developed from the offspring of vsg x ('tm-1' x 'hai7124'). vsg is a virescently marked semigamy line of gossypi ...200516121235
measuring gene flow in the cultivation of transgenic cotton (gossypium hirsutum l.).transgenic bt cotton newcott 33b and transgenic tfd a cotton tfd were chosen to evaluate pollen dispersal frequency and distance of transgenic cotton (gossypium hirsutum l.) in the huanghe valley cotton-producing zone, china. the objective was to evaluate the efficacy of biosafety procedures used to reduce pollen movement. a field test plot of transgenic cotton (6 x 6 m) was planted in the middle of a nontransgenic field measuring 210 x 210 m. the results indicated that the pollen of bt cotton o ...200516118411
broiler litter as a micronutrient source for cotton: concentrations in plant parts.analytically, poultry litter contains nearly all essential micronutrients but the extent of phytoavailability of these nutrients and whether cotton (gossypium hirsutum l.) and other crop plants can receive adequate amounts of these nutrients from litter is not fully known. the objective of this research was to determine whether cotton receives sufficient amounts of fe, cu, mn, and zn from litter and estimate the efficiency of cotton in extracting these metal nutrients from litter in the absence ...200616091623
isolation and characterization of drought-related trehalose 6-phosphate-synthase gene from cultivated cotton (gossypium hirsutum l.).due to the important role of cotton drought-tolerant varieties and the reported involvement in this trait of trehalose-6-phosphate-synthase, the respective gene (tps) was isolated and characterized from cultivated cotton, gossypium hirsutum (zeta 2 cultivar), using a chromosome-walking technique. tps has three exons comprising the coding region. southern blot analysis indicated that the gossypium genomes (a and d) contain a single copy of tps per genome. in addition, the expression of this gene ...200616086175
ghhb1: a nonsymbiotic hemoglobin gene of cotton responsive to infection by verticillium dahliae.verticillium wilt of cotton is a widespread and destructive disease that is caused by the fungus pathogen verticillium dahliae. although no cotton cultivar is immune to the disease, some genotypes exhibit superior wilt tolerance. to gain an insight into the molecular mechanisms responsible for wilt tolerance, we employed the method of suppression subtractive hybridization (ssh) to isolate genes whose expression is up-regulated after inoculation of the pathogen in a wilt-tolerant cotton cultivar ...200516084605
occurrence of (+)- and (-)-gossypol in wild species of cotton and in gossypium hirsutum var. marie-galante (watt) hutchinson.gossypol occurs as a mixture of enantiomers in cottonseed. these enantiomers exhibit different biological activities. the (-)-enantiomer is toxic to animals, but it has potential medicinal uses. therefore, cottonseed with >95% (-)-gossypol could have biopharmaceutical applications. the (+)-enantiomer shows little, if any, toxicity to nonruminant animals. thus, cottonseed with >95% (+)-gossypol could be more readily utilized as a feed for nonruminants. the (+)- to (-)-gossypol ratio in commercial ...200516076104
genetic mapping of a cross between gossypium hirsutum (cotton) and the hawaiian endemic, gossypium tomentosum.the existence of five tetraploid species that derive from a common polyploidization event about 1 million years ago makes gossypium (cotton) an attractive genus in which to study polyploid evolution and offers opportunities for crop improvement through introgression. to date, only crosses (hb) between the cultivated tetraploid cottons gossypium hirsutum and g. barbadense have been genetically mapped. genetic analysis of a cross (ht) between g. hirsutum and the hawaiian endemic g. tomentosum is r ...200516044266
activity of selected neonicotinoids and dicrotophos on nontarget arthropods in cotton: implications in insect management.certain neonicotinoids are used in cotton, gossypium hirsutum (l.), to control various piercing-sucking pests. we conducted field studies using three neonicotinoids (acetamiprid, thiamethoxam, and imidacloprid) and an organophosphate (dicrotophos) to assess the activity of these insecticides against nontarget arthropods, particularly predators, and to determine the potential economic consequences of such activity. mortality among populations of the big-eyed bug, geocoris punctipes (say), and the ...200516022310
effects of planting dates on boll weevils (coleoptera: curculionidae) and cotton fruit in the subtropics.the effects of planting dates 2-3-wk apart on boll weevil, anthonomus grandis grandis boheman (coleoptera: curculionidae), field-level populations, and feeding and oviposition damage to cotton, gossypium hirsutum l., squares and bolls, were studied during 2002 and 2003 in the lower rio grande valley of texas. squares were 44-56% more abundant in some later planted treatments than in the earlier planted treatments, but mean cumulative numbers of oviposition- and feeding-damaged squares were 2.7 - ...200516022308
size-dependent feeding and reproduction by boll weevil (coleoptera: curculionidae).the considerable variation in adult size of the boll weevil, anthonomus grandis grandis boheman, has been well documented, but the influences of adult size on reproductive rate are not known. we examined the relationship between the size of boll weevils and their feeding and oviposition. weevils weighed to the nearest milligram were grouped into five categories based on pupal weight: < or =5, 6-10, 11-15, 16-20, and >20 mg. numbers of lifetime punctures produced in flower buds (squares) of cotto ...200516022302
functional characterization of gossypium hirsutum profilin 1 gene (ghpfn1) in tobacco suspension cells. characterization of in vivo functions of a cotton profilin gene.cotton fiber is an extremely long plant cell. fiber elongation is a complex process and the genes that are crucial for elongation are largely unknown. we previously cloned a cdna encoding an isoform of cotton profilin and found that the gene (designated ghpfn1) was preferentially expressed in cotton fibers. in the present study, we have further analyzed the expression pattern of ghpfn1 during fiber development and studied its cellular function using tobacco suspension cells as an experimental sy ...200516001260
agricultural dust production in standard and conservation tillage systems in the san joaquin valley.the negative health effects of repeated dust exposure have been well documented. in california's san joaquin valley, agricultural operations may contribute substantially to airborne particulates. we evaluated four management systems to assess impacts on dust production and soil properties for a cotton (gossypium hirsutum l.)-tomato (lycopersicon esculentum mill.) rotation: standard tillage with (stcc) and without (stno) cover crop, and conservation tillage with (ctcc) and without (ctno) cover cr ...200615998847
molecular dissection of interspecific variation between gossypium hirsutum and g. barbadense (cotton) by a backcross-self approach: ii. fiber fineness.a backcross-self population from a cross between gossypium hirsutum and g. barbadense was used to dissect the molecular basis of genetic variation governing two parameters reflecting lint fiber fineness and to compare the precision of these two measurements. by applying a detailed restriction fragment length polymorphism (rflp) map to 3,662 bc(3)f(2) plants from 24 independently derived bc(3) families, we were able to detect 32 and nine quantitative trait loci (qtls) for fiber fineness and micro ...200515995865
molecular dissection of phenotypic variation between gossypium hirsutum and gossypium barbadense (cotton) by a backcross-self approach: iii. fiber length.a backcross-self population from a cross between gossypium hirsutum and g. barbadense was used to dissect the molecular basis of genetic variation governing 15 parameters that reflect fiber length. applying a detailed restriction fragment length polymorphism (rflp) map to 3,662 bc(3)f(2) plants from 24 independently derived bc(3) families, we detected 28, nine, and eight quantitative trait loci (qtls) for fiber length, length uniformity, and short fiber content, respectively. for eight, six, and ...200515983757
molecular dissection of interspecific variation between gossypium hirsutum and gossypium barbadense (cotton) by a backcross-self approach: i. fiber elongation.the current study is the first installment of an effort to explore the secondary gene pool for the enhancement of upland cotton (gossypium hirsutum l.) germplasm. we developed advanced-generation backcross populations by first crossing g. hirsutum cv. tamcot 2111 and g. barbadense cv. pima s6, then independently backcrossing f(1) plants to the g. hirsutum parent for three cycles. genome-wide mapping revealed introgressed alleles at an average of 7.3% of loci in each bc(3)f(1) plant, collectively ...200515983756
okra-leaf accessions of the upland cotton (gossypium hirsutum l.): genetic variability in agronomic and fibre traits.okra-leaf types of the upland cotton have the potential to be competitive to the normal-leaf types in yield and fibre quality, in addition to its potential resistance to insect pests and drought. okra-leaf cotton accessions, collected at cotton research institute, faisalabad, pakistan, were evaluated in respect of genetic variance and relative performance in half- and full-sib crosses (combining ability) for 2 years. variation due to parents x years interaction was significant for lint percentag ...200515977325
evaluation of transgenic cotton varieties and a glyphosate application on seedling disease incidence.a study was conducted to determine whether stand densities of transgenic cotton (gossypium hirsutum) varieties, with or without glyphosate, were similar to conventional varieties of the same lineage group in georgia and mississippi. transgenic and conventional cotton varieties were placed into five lineage groups of related varieties and seedling disease was evaluated in three greenhouse tests and a field trial using rhizoctonia solani ag-4. seed vigor was determined by standard germination stud ...200515973787
molecular cloning and characterization of a cotton glucuronosyltranferase gene.a glucuronosyltranferase gene has been isolated from cotton (gossypium hirsutum) fiber cells using rapid amplification of the cdna ends. the full-length cdna, designated ghglcat1, is 1400 bp in length (ay346330) and contains an open reading frame of 1107 bp encoding a protein of 368 amino acids. alignment of the ghglcat1 predicted amino acid sequence was shown to have high sequence similarity with animal glucuronosyltranferases. a phylogenic tree generated by the phylip program package showed th ...200515940874
successive chromosome walking by compatible ends ligation inverse pcr.here we describe an advanced polymerase chain reaction (pcr) technique, the compatible ends ligation inverse pcr (celi-pcr) for chromosome walking. in celi-pcr, several restriction enzymes, which produce compatible cohesive ends, were used to digest target dna simultaneously or sequentially to produce dna fragments of suitable size. dna fragments were then easily circularized and pcr amplification could be carried out efficiently. the previous limitations of inverse pcr were overcome, such as un ...200515920279
effects of potassium humate solution and soaking periods on germination characteristics of undelinted cotton seeds (gossypium hirsutum l.).the present study was conducted to determine the effects of soaking in potassium humate (ph) solution (55% humic acid, 30% fulvic acid and 8% potassium hydroxide) and distilled water (dw) for different periods (0, 4, 8 and 16 hours) on germination characteristics of undelinted seeds of cotton cv ersan-92. radicle, hypocotyl and seedling length, radicle and hypocotyl elongation rate and vigor index increased by ph solution treatment and increasing soaking periods. the medium x soaking period inte ...200415907066
systemic induction of volatile release in cotton: how specific is the signal to herbivory?plants attacked by herbivorous insects release chemical signals that attract natural enemies of the herbivores to the damaged plants. feeding of spodoptera exigua larvae on the lower leaves of cotton (gossypium hirsutum l.) for multiple feeding periods of 9-12 h with a 12 h, interval in between when the caterpillars are removed overnight, will induce a systemic release of volatile compounds that is comparable to the volatiles released in response to continuous feeding damage on the lower leaves ...200515856281
antisense suppression of a (+)-delta-cadinene synthase gene in cotton prevents the induction of this defense response gene during bacterial blight infection but not its constitutive expression.in cotton (gossypium hirsutum) the enzyme (+)-delta-cadinene synthase (cdns) catalyzes the first committed step in the biosynthesis of cadinane-type sesquiterpenes, such as gossypol, that provide constitutive and inducible protection against pests and diseases. a cotton cdna clone encoding cdns (cdn1-c4) was isolated from developing embryos and functionally characterized. southern analysis showed that cdns genes belong to a large multigene family, of which five genomic clones were studied, inclu ...200515849309
transgenic cotton (gossypium hirsutum l.) seedlings expressing a tobacco glutathione s-transferase fail to provide improved stress tolerance.transgenic cotton (gossypium hirsutum l.) lines expressing the tobacco glutathione s-transferase (gst) nt107 were evaluated for tolerance to chilling, salinity, and herbicides, antioxidant enzyme activity, antioxidant compound levels, and lipid peroxidation. although transgenic seedlings exhibited ten-fold and five-fold higher gst activity under normal and salt-stress conditions, respectively, germinating seedlings did not show improved tolerance to salinity, chilling conditions, or herbicides. ...200515824906
validation of a cotton-specific gene, sad1, used as an endogenous reference gene in qualitative and real-time quantitative pcr detection of transgenic cottons.genetically modified (gm) cotton lines have been approved for commercialization and widely cultivated in many countries, especially in china. as a step towards the development of reliable qualitative and quantitative pcr methods for detecting gm cottons, we report here the validation of the cotton (gossypium hirsutum) endogenous reference control gene, sad1, using conventional and real-time (rt)-pcr methods. both methods were tested on 15 different g. hirsutum cultivars, and identical amplicons ...200515726375
resistance gene analogue markers are mapped to homeologous chromosomes in cultivated tetraploid cotton.degenerate primers designed from conserved motifs of known plant resistance gene products were used to amplify genomic dna sequences from the root-knot nematode (meloidogyne incognita) resistance genetic source, upland cotton (gossypium hirsutum) cultivar auburn 634 rnr. a total of 165 clones were isolated, and sequence analysis revealed 57 of the clones to be novel nucleotide sequences, many containing the resistance (r)-protein nucleotide-binding site motif. a cluster analysis was performed wi ...200515726317
the cotton actin1 gene is functionally expressed in fibers and participates in fiber elongation.single-celled cotton fiber (gossypium hirsutum) provides a unique experimental system to study cell elongation. to investigate the role of the actin cytoskeleton during fiber development, 15 g. hirsutum actin (ghact) cdna clones were characterized. rna gel blot and real-time rt-pcr analysis revealed that ghact genes are differentially expressed in different tissues and can be classified into four groups. one group, represented by ghact1, is expressed predominantly in fiber cells and was studied ...200515722467
[cloning and expression analysis of two rac genes from cotton (gossypium hirsutum l.)].plant rac proteins belong to an important group of signal switches anchoring on membranes, involved in various physiological processes including cell polar growth, synthesis of secondary wall, resistance response and hormone signaling. in the attempt to elucidate the molecular mechanism of initiation and elongation of cotton fiber, two cotton rac protein genes, designated as ghraca and ghracb, were amplified from elongating fibers and cloned. it was demonstrated that, the cdna of ghraca containe ...200515715441
the delayed initiation and slow elongation of fuzz-like short fibre cells in relation to altered patterns of sucrose synthase expression and plasmodesmata gating in a lintless mutant of cotton.cotton (gossypium hirsutum l.) seed develops single-celled long fibres (lint) from the seed-coat epidermis at anthesis. previous studies have shown that the initiation and rapid elongation of these fibres requires the expression of sucrose synthase (sus) and, potentially, a transient closure of plasmodesmata. this study extends the previous work to examine the patterns of sus expression and plasmodesmata gating in fuzz-like short fibres of a mutant that shows delayed initiation and much slower a ...200515710635
combined effects of elevated co2 and herbivore damage on alfalfa and cotton.we examined herbivore-induced responses of alfalfa (medicago sativa) and cotton (gossypium hirsutum) under different co2 conditions. plants were grown under ambient (350 ppm) or elevated (700 ppm) co2 levels, and were either damaged or undamaged by spodoptera littoralis larvae. at harvest, growth of undamaged (control) plants was determined, and foliar chemical composition of both undamaged and damaged plants was analyzed. cotton grew faster overall and showed a greater increase in growth in res ...200415672673
boll injury and yield losses in cotton associated with brown stink bug (heteroptera: pentatomidae) during flowering.brown stink bug, euschistus servus (say), was infested on cotton, gossypium hirsutum l., plants during reproductive stages to determine the effects on boll injury and seedcotton yield. during each week in 2002 and 2003, significantly more bolls with > or = 1 injured locule, bolls with > or = 2 injured locules, and bolls with discolored lint were recorded on stink bug-infested plants compared with that on noninfested plants. significantly fewer bolls displayed external injury on the boll exocarp ...200415666747
confirmation and quantification of strigolactones, germination stimulants for root parasitic plants striga and orobanche, produced by cotton.the germination stimulants for root parasitic plants striga and orobanche produced by cotton (gossypium hirsutum l.) were examined in detail. seeds of cotton were germinated and grown on glass wool wetted with sterile distilled water in sterile filter units. the root exudate was collected daily and extracted with ethyl acetate. each of these ethyl acetate extracts was analyzed directly by high-performance liquid chromatography linked with tandem mass spectrometry (lc/ms/ms). the results demonstr ...200515665473
toxicity to cotton boll weevil anthonomus grandis of a trypsin inhibitor from chickpea seeds.cotton (gossypium hirsutum l.) is an important agricultural commodity, which is attacked by several pests such as the cotton boll weevil anthonomus grandis. adult a. grandis feed on fruits and leaf petioles, reducing drastically the crop production. the predominance of boll weevil digestive serine proteinases has motivated inhibitor screenings in order to discover new ones with the capability to reduce the digestion process. the present study describes a novel proteinase inhibitor from chickpea ...200515649779
evaluation of transgenic cotton varieties and a glyphosate application on seedling disease incidence.a study was conducted to determine whether stand densities of transgenic cotton (gossypium hirsutum) varieties, with or without glyphosate, were similar to conventional varieties of the same lineage group in georgia and mississippi. transgenic and conventional cotton varieties were placed into five lineage groups of related varieties and seedling disease was evaluated in three greenhouse tests and a field trial using rhizoctonia solani ag-4. seed vigor was determined by standard germination stud ...200415645175
post-transcriptional gene silencing induced by short interfering rnas in cultured transgenic plant cells.short interfering rna (sirna) is widely used for studying post-transcriptional gene silencing and holds great promise as a tool for both identifying function of novel genes and validating drug targets. two sirna fragments (sirna-a and -b), which were designed against different specific areas of coding region of the same target green fluorescent protein (gfp) gene, were used to silence gfp expression in cultured gfp transgenic cells of rice (oryza sativa l.; os), cotton (gossypium hirsutum l.; gh ...200415629049
dexamethasone-inducible green fluorescent protein gene expression in transgenic plant cells.genomic research has made a large number of sequences of novel genes or expressed sequence tags available. to investigate functions of these genes, a system for conditional control of gene expression would be a useful tool. inducible transgene expression that uses green fluorescent protein gene (gfp) as a reporter gene has been investigated in transgenic cell lines of cotton (cot; gossypium hirsutum l.), fraser fir [fra; abies fraseri (pursh) poir], nordmann fir (nor; abies nordmanniana lk.), an ...200415629039
chromosomal assignment of rflp linkage groups harboring important qtls on an intraspecific cotton (gossypium hirsutum l.) joinmap.chromosome identities were assigned to 15 linkage groups of the rflp joinmap developed from four intraspecific cotton (gossypium hirsutum l.) populations with different genetic backgrounds (acala, delta, and texas plains). the linkage groups were assigned to chromosomes by deficiency analysis of probes in the previously published joinmap, based on genomic dna from hypoaneuploid chromosome substitution lines. these findings were integrated with qtl identification for multiple fiber and yield trai ...201015618303
[cloning and characterization of two beta-mannosidase cdnas in gossypium hirsutum l].by using the method of pcr-based cdna library screening, two beta-mannosidase clones, ghmana1 and ghmana2, had been isolated. ghmana1 had a length of 2692 bp coding for a polypeptide of 834 amino acids, and ghmana2 was 3209 bp which encoded a polypeptide of 976 amino acids. ghmana1 and ghmana2 shared an identical sequence of 747 amino acids in their carboxyl-terminals, but were distinctly different in their amino-terminals. both beta-mannosidases were members of glycosyl hydrolase family 2, whic ...200415599050
[glyphosate-resistant cotton (gossypium hirsutum l.) transformed with aroam12 gene via agrobacterium tumefaciens].a mutant, aroam12, exhibiting resistance to glyphosate produced in a previous study using the staggered extension process with aroa genes from salmonella typhimurium and eschrichia coli. in this paper, we constructed a vector pgra1300 carrying aroam12 gene, comprising transit peptide of arabidopsis epsps, under the control of the camv35s promoter and used as selectable marker for cotton plant (gossypium hirsutum l.) transformation. transgenic cottons with increased resistance to glyphosate were ...200415599043
sts markers linked to the rf1 fertility restorer gene of cotton.marker-assisted selection (mas) can accelerate the process of plant breeding, and sequence-tagged site (sts) markers are highly specific for regions of dna being used for mas. the objective of this research was to develop sts markers tightly linked with rf1, the fertility restoring gene for cytoplasmic male sterility (cms) in cotton (gossypium hirsutum l.). bulked segregant analysis was employed to screen for rf1-linked rapd markers in a backcross population. four rapd markers were identified, t ...200515592810
genetic diversity and geographic pattern in early south american cotton domestication.amplified fragment length polymorphism fingerprinting was applied to survey the genetic diversity of primitive south american gossypium barbadense cotton for establishing a possible link to its pre-columbian expansion. new germplasm was collected along coastal peru and over an andean transect in areas where most of the archaeological evidence relating to cotton domestication has been recorded. gene bank material of three diploid (g. raimondii, g. arboreum, and g. herbaceum) and four allotetraplo ...200515580473
an internal motor kinesin is associated with the golgi apparatus and plays a role in trichome morphogenesis in arabidopsis.members of the kinesin superfamily are microtubule-based motor proteins that transport molecules/organelles along microtubules. we have identified similar internal motor kinesins, kinesin-13a, from the cotton gossypium hirsutum and arabidopsis thaliana. their motor domains share high degree of similarity with those of internal motor kinesins of animals and protists in the mcak/kinesin13 subfamily. however, no significant sequence similarities were detected in sequences outside the motor domain. ...200515574882
relative concentration of cry1a in maize leaves and cotton bolls with diverse chlorophyll content and corresponding larval development of fall armyworm (lepidoptera: noctuidae) and southwestern corn borer (lepidoptera: crambidae) on maize whorl leaf profiles.to manage insect resistance to transgenic crops that express insecticidal proteins from bacillus thuringiensis (bt) berliner, the u.s. environmental protection agency recommends a refuge-based insect resistance management strategy where a percentage of non-bt (refuge) crop is grown in proximity to a bt-expressing crop. an important requirement for this strategy is that the toxin exists at a high effective dose for control of the target pest(s), so that heterozygous individuals in the population ...200415568367
comparative production of helicoverpa zea (lepidoptera: noctuidae) from transgenic cotton expressing either one or two bacillus thuringiensis proteins with and without insecticide oversprays.transgenic cotton, gossypium hirsutum (l.), expressing either one or two bacillus thuringiensis ssp. kurstaki berliner (bt) proteins was compared with the conventional sister line in field experiments with regard to production of bollworm, helicoverpa zea (boddie), and bolls damaged by bollworm. the relative numbers of bollworms that developed on bollgard (monsanto co., st. louis, mo), bollgard ii (monsanto co.), and conventional cotton were estimated under nontreated conditions in 2000 and both ...200415568364
genotypic and developmental evidence for the role of plasmodesmatal regulation in cotton fiber elongation mediated by callose turnover.cotton fibers are single-celled hairs that elongate to several centimeters long from the seed coat epidermis of the tetraploid species (gossypium hirsutum and gossypium barbadense). thus, cotton fiber is a unique system to study the mechanisms of rapid cell expansion. previous work has shown a transient closure of plasmodesmata during fiber elongation (y.-l. ruan, d.j. llewellyn, r.t. furbank [2001] plant cell 13: 47-60). to examine the importance of this closure in fiber elongation, we compared ...200415557097
a plant-specific kinesin binds to actin microfilaments and interacts with cortical microtubules in cotton fibers.a novel kinesin, ghkch1, has been identified from cotton (gossypium hirsutum) fibers. ghkch1 has a centrally located kinesin catalytic core, a signature neck peptide of minus end-directed kinesins, and a unique calponin homology (ch) domain at its n terminus. ghkch1 and other ch domain-containing kinesins (kchs) belong to a distinct branch of the minus end-directed kinesin subfamily. to date the kch kinesins have been found only in higher plants. because the ch domain is often found in actin-bin ...200415557092
cloning, characterization, and evolution of the nbs-lrr-encoding resistance gene analogue family in polyploid cotton (gossypium hirsutum l.).the nucleotide-binding site-leucine-rich repeat (nbs-lrr)-encoding gene family has attracted much research interest because approximately 75% of the plant disease resistance genes that have been cloned to date are from this gene family. we cloned the nbs-lrr-encoding genes from polyploid cotton by a polymerase chain reaction-based approach. a sample of 150 clones was selected from the nbs-lrr gene sequence library and was sequenced, and 61 resistance gene analogs (rga) were identified. sequence ...200415553248
[cloning of a mads box protein gene (ghmads1) from cotton (gossypium hirsutum l.)].as a kind of transcription factors, mads-box protein plays an important role in various cellular processes, especially in the development of floral organs. based on the contig analysis of the cotton ests, the coding region of a cotton mads-box protein (ghmads1) was obtained by rt-pcr from floral buds of cotton (g. hirsutum). the cloned fragment of 713 bp (ghmads1, genbank accession no. af538965) contains an open reading frame of 711 bp,coding a polypeptide of 236 amino acids. it was demonstrated ...200415552050
fluometuron and pendimethalin runoff from strip and conventionally tilled cotton in the southern atlantic coastal plain.in the atlantic coastal plain region of southern georgia (usa), cotton (gossypium hirsutum l.) acreage increased threefold in the past decade. to more effectively protect water quality in the region, best management practices are needed that reduce pesticide runoff from fields in cotton production. this study compared runoff of two herbicides, fluometuron [n,n-dimethyl-n'-[3-(trifluoromethyl)-phenyl]-urea] and pendimethalin [n-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitro-benzenamine], from plots in ...201515537934
bollgard ii cotton: compositional analysis and feeding studies of cottonseed from insect-protected cotton (gossypium hirsutum l.) producing the cry1ac and cry2ab2 proteins.bollgard ii cotton event 15985 producing the cry1ac and cry2ab2 proteins has been developed by genetic modification to broaden the spectrum of insects to which the plant is tolerant and to provide an insect resistance management tool to impede the onset of resistance. the purpose of this study was to evaluate the composition and nutrition of bollgard ii cotton, relative to the use for food and animal feed, compared to that of conventional cotton varieties. compositional analyses were conducted t ...200415537305
cloning and expression analysis of a bzip cdna in gossypium hirsutum l.a new full-length cdna clone was isolated from the fiber cdna library of gossypium hirsutum l. the cdna, designated ghbzip,encoded a polypeptide of 645 amino acids. ghbzip protein had the structure characteristics of plant bzip proteins, including two conserved domains, duf630 and duf632 with unknown functions, a proline-rich domain and a phenylalanine-rich domain. meanwhile, the protein contained a leucine zipper-like motif in duf632. the hydropathy analysis showed that the ghbzip was a membran ...200415490881
[the structural characteristics, alternative splicing and genetic experession analysis of adp-ribosylation-factor 1 (arf1) in cotton].the full-length cdna,dna and promoter of adp-ribosylation-factor 1 (arf1) was isolated from gossypium hirsutum y18 by means of isocaudarner inverse pcr (ii-pcr) and rapid isolating cdna 5' unknown sequence and promoter (ricup) established in our lab. results indicated that the gene is 4 360 bp in size, including seven exons and six introns. interestingly, alterative splicing occurs at intron i. differential processing of intron 1 yields three different transcripts with 1 026 bp, 1103 bp and 1 54 ...200415481542
effects of farmyard manure and fertilizers on yield, fibre quality and nutrient balance of rainfed cotton (gossypium hirsutum).two-year field experiments were conducted to evaluate the effect of fertilizer with or without farmyard manure (fym) application on cotton productivity and fibre quality. a partial nutrient balance was calculated by the difference method (nutrient applied--crop removal). seed cotton yield was improved with addition of fym (5 mg ha(-1)). application of both n and p resulted in significant improvements in seed cotton yield than the control and without n plots (pk). uniformity ratio and ginning out ...200515474936
msma resistance studies.monosodium methanearsonate (msma)-resistant and -susceptible common cocklebur (xanthium strumarium l.) and cotton (gossypium hirsutum l.) were treated with msma. plant parameters analyzed were: glutathione synthetase activity, selected amino acid (arginine, glutamic acid, alanine, citrulline, glutamine, and glutathione) content and arsenic content (msma, total arsenic, and arsonate). no reduction of arsenic from the parent pentavalent form present in msma to the trivalent form was detected. argi ...200415473645
cloning and expression analysis of carboxyl-terminal protease cdna from gossypium hirsutum l.a full-length cotton cdna,designated ghctp, was isolated from gossypium hirsutum l. by the pcr-based cdna library screening. the cdna encodes a polypeptide of 473-amino acid residues. sequence alignment showed that ghctp was highly homologous to a family of carboxyl-terminal proteases (ctp) from other species. ghctp is a potential transmembrane protease, and comprises a conserved domain numbered duf239 at its carboxyl-terminal and an arginine-rich region at the amino-terminal, respectively. furt ...200415473308
influence of cover crops on insect pests and predators in conservation tillage cotton.in fall 2000, an on-farm sustainable agricultural research project was established for cotton, gossypium hirsutum l., in tift county, georgia. the objective of our 2-yr research project was to determine the impact of several cover crops on pest and predator insects in cotton. the five cover crop treatments included 1) cereal rye, secale cereale l., a standard grass cover crop; 2) crimson clover, trifolium incarnatum l., a standard legume cover crop; 3) a legume mixture of balansa clover, trifoli ...200415384330
identification and quantification of gossypol in cotton by using packed micro-tips columns in combination with hplc.self-packed micro-tip columns containing a c18-bonded silica stationary phase, based on the same principles as solid-phase extraction methods, were used to obtain gossypol and related sesquiterpenoid aldehyde-enriched fractions. the enriched metabolite fractions were then analyzed by optimized high-performance liquid chromatography (hplc) with a c18 column (4.6 mm x 25 cm) eluted with the binary mobile phase acetonitrile-0.1% aqueous tfa solution (80:20). this method has proven to be highly repr ...200415372136
characterization of ghrac1 gtpase expressed in developing cotton (gossypium hirsutum l.) fibers.cytoskeleton assembly plays an important role in determining cotton fiber cell length and morphology and is developmentally regulated. as in other plant cells, it is not clear how cytoskeletal assembly in fibers is regulated. recently, several rac/rop gtpases in arabidopsis were shown to regulate isotropic and polar cell growth of root hairs and pollen tubes by controlling assembly of the cytoskeleton. ghrac1, isolated from cottonseeds, is a member of the rac/rop gtpase family and is abundantly ...200415358513
enzyme activities and arylsulfatase protein content of dust and the soil source: biochemical fingerprints?little is known about the potential of enzyme activities, which are sensitive to soil properties and management, for the characterization of dust properties. enzyme activities may be among the dust properties key to identifying the soil source of dust. we generated dust (27 and 7 microm) under controlled laboratory conditions from agricultural soils (0-5 cm) with history of continuous cotton (gossypium hirsutum l.) or cotton rotated with peanut (arachis hypogaea l.), sorghum [sorghum bicolor (l. ...200615356225
molecular cloning and characterization of ghlecrk, a novel kinase gene with lectin-like domain from gossypium hirsutum.a novel gene encoding a lectin-like protein kinase was cloned from the upland cotton (gossypium hirsutum) through cdna library screening. this gene (named as ghlecrk; genbank accession number: ay487461) had a total length of 2233bp with an open reading frame of 1926bp, and encoded a predicted polypeptide of 641 amino acids with a molecular weight of 71.16kda. the ghlecrk protein shared 73, 65, 64 and 59% identity with other lectin-like kinase proteins isolated from a. thaliana (at3g53810, at2g37 ...200415354356
low salt petroleum produced water reuse: a farming alternative outside the food chain.oil and gas production gives rise to water production depending on the state of maturation of the field. this means large volumes of water available. today, this water is partly re-injected into the reservoir. totalfinaelf's sustainable contribution to preserve water resources is to propose an alternative utilization: the reuse of produced waters from petroleum activities, outside the food chain. the aim of the first part of this study is to demonstrate the feasibility of utilizing low-salt wate ...200415344784
an optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton.a comparative study on gossypol content of various genetic types of pigment glands of cotton varieties was conducted through an optimized high-performance liquid chromatography (hplc) on a c18 column (4.6 mm x 250 mm, 5 microm particle) with methanol-0.5% acetic acid aqueous solution, 90 : 10 (v/v), as mobile phase, at a flow rate of 0.8 ml/min and uv detection at 254 nm. the method was shown to be highly reproducible, with precision [as relative standard deviation (rsd)] and accuracy [as relati ...200415286405
members of a new group of chitinase-like genes are expressed preferentially in cotton cells with secondary walls.two homologous cotton (gossypium hirsutum l.) genes, ghctl1 and ghctl2, encode members of a new group of chitinase-like proteins (called the ghctl group) that includes other proteins from two cotton species, arabidopsis, rice, and pea. members of the ghctl group are assigned to family gh19 glycoside hydrolases along with numerous authentic chitinases (http://afmb.cnrs-mrs.fr/cazy/index.html), but the proteins have novel consensus sequences in two regions that are essential for chitinase activity ...200415284492
wide-cross whole-genome radiation hybrid mapping of cotton (gossypium hirsutum l.).we report the development and characterization of a "wide-cross whole-genome radiation hybrid" (wwrh) panel from cotton (gossypium hirsutum l.). chromosomes were segmented by gamma-irradiation of g. hirsutum (n = 26) pollen, and segmented chromosomes were rescued after in vivo fertilization of g. barbadense egg cells (n = 26). a 5-krad gamma-ray wwrh mapping panel (n = 93) was constructed and genotyped at 102 ssr loci. ssr marker retention frequencies were higher than those for animal systems an ...200415280245
injury to preflowering and flowering cotton by brown stink bug and southern green stink bug.the impact of brown stink bug, euschistus servus (say), and southern green stink bug, nezara viridula (l.), injury was evaluated on preflowering and flowering cotton, gossypium hirsutum l., plants in no-choice tests. vegetative stage cotton seedlings and reproductive structures, including flower buds (square) and bolls, were infested with adults and/or nymphs of both species. there were no significant differences in height, height to node ratio, square retention, and flower initiation for cotton ...200415279273
evaluation of haemoglobin (erythrogen): for improved somatic embryogenesis and plant regeneration in cotton (gossypium hirsutum l. cv. svpr 2).somatic embryogenesis in cotton (gossypium hirsutum l.) is accelerated when the plant regeneration medium is supplemented with haemoglobin (erythrogen). in cotton svpr 2 lines, a higher frequency of embryoid formation was observed when the medium contained 400 mg/l haemoglobin. fresh weight of the callus, rate of embryoid induction, number of embryoids formed and the percentage of plant regeneration from somatic embryos were increased. among the two different cultivars tested, mcu 11 showed no r ...200415278417
incongruent patterns of local and global genome size evolution in cotton.genome sizes in plants vary over several orders of magnitude, reflecting a combination of differentially acting local and global forces such as biases in indel accumulation and transposable element proliferation or removal. to gain insight into the relative role of these and other forces, approximately 105 kb of contiguous sequence surrounding the cellulose synthase gene cesa1 was compared for the two coresident genomes (at and dt) of the allopolyploid cotton species, gossypium hirsutum. these t ...200415256507
terpenoid aldehyde formation and lysigenous gland storage sites in cotton: variant with mature glands but suppressed levels of terpenoid aldehydes.a new cotton variant with reduced levels of terpenoid aldehydes (sesquiterpenoids and sesterterpenoids (heliocides)) was isolated from the progeny of hemizygous cotton (gossypium hirsutum cv. coker 312) transformed with antisense (+)-delta-cadinene synthase cdna. southern analysis of leaf dna digested with hindiii, pst or kpni restriction endonucleases did not detect any antisense cdn1-c1 dna in the genome of the variant. the gossypol content in the seed of the variant was markedly lower than in ...200415231408
widespread paleopolyploidy in model plant species inferred from age distributions of duplicate genes.it is often anticipated that many of today's diploid plant species are in fact paleopolyploids. given that an ancient large-scale duplication will result in an excess of relatively old duplicated genes with similar ages, we analyzed the timing of duplication of pairs of paralogous genes in 14 model plant species. using est contigs (unigenes), we identified pairs of paralogous genes in each species and used the level of synonymous nucleotide substitution to estimate the relative ages of gene dupl ...200415208399
leaf and canopy photosynthetic characteristics of cotton (gossypium hirsutum) under elevated co2 concentration and uv-b radiation.increases in both atmospheric co2 concentration ([co2]) and ultraviolet-b (uv-b) radiation on the earth's surface are features of current climate change patterns. an experiment was conducted in sunlit, controlled environment chambers known as soil-plant-atmosphere-research (spar) units to determine interactive effects of elevated [co2] and uv-b radiation on leaf and canopy photosynthetic characteristics of cotton. six treatments were comprised of two co2 levels of 360 (ambient) and 720 (elevated ...200415202715
comparative proteomics of cannabis sativa plant tissues.comparative proteomics of leaves, flowers, and glands of cannabis sativa have been used to identify specific tissue-expressed proteins. these tissues have significantly different levels of cannabinoids. cannabinoids accumulate primarily in the glands but can also be found in flowers and leaves. proteins extracted from glands, flowers, and leaves were separated using two-dimensional gel electrophoresis. over 800 protein spots were reproducibly resolved in the two-dimensional gels from leaves and ...200415190082
the cotton ghnhx1 gene encoding a novel putative tonoplast na(+)/h(+) antiporter plays an important role in salt stress.a cdna clone was isolated from cotton (gossypium hirsutum) cdna library and characterized with regard to its sequence, regulation in response to salt stress and functions in yeast mutants and transgenic tobacco plants. the clone, designated as ghnhx1, contains 2485 nucleotides with an open reading frame of 1629 nucleotides, and the deduced amino acid sequence showed high identities with other plant vacuolar-type na(+)/h(+) antiporters. northern blot analysis indicated that the mrna accumulation ...200415169942
characterization of the brassinosteroid insensitive 1 genes of cotton.suppression of brassinosteroid (br) biosynthesis in cotton ovules by treatment with brassinazole inhibits fiber formation, indicating that br plays an important role in cotton fiber development. plant responses to brassinosteroids (br) are mediated through a plasma membrane-bound leucine-rich repeat (lrr) receptor-like protein kinase known as bri1. mutations in the bri1 genes of several species result in dwarfed plants with reduced sensitivity to br. a single expressed sequence tag (est) from co ...200415159624
recovery of leaf area through accelerated shoot ontogeny in thrips-damaged cotton seedlings.leaf area of cotton seedlings (gossypium hirsutum) can be reduced by as much as 50 % by early season thrips infestations, but it is well documented that plants can regain the difference in leaf area once infestation ceases. the processes involved in the recovery have not been identified. hypotheses include enhancement of the photosynthetic rate of the damaged leaves, more efficient leaf construction (i.e. more leaf area per unit of dry matter invested in new leaves), and more branching.200415155380
effects of burial and soil condition on postharvest mortality of boll weevils (coleoptera: curculionidae) in fallen cotton fruit.effects of soil condition and burial on boll weevil, anthonomus grandis grandis boheman, mortality in fallen cotton, gossypium hirsutum l., fruit were assessed in this study. during hot weather immediately after summer harvest operations in the lower rio grande valley of texas, burial of infested fruit in conventionally tilled field plots permitted significantly greater survival of weevils than in no-tillage plots. burial of infested squares protected developing weevils from heat and desiccation ...200415154462
effects of nematicides on cotton root mycobiota.baseline information on the diversity and population densities of fungi collected from soil debris and cotton (gossypium hirsutum l.) roots was determined. samples were collected from tifton, ga, and starkville, ms containing cotton field soil treated with the nematicides 1,3-dichloroproprene (fumigant) and aldicarb (granules). a total of 10,550 and 13,450 fungal isolates were collected from these two study sites, respectively. of this total, 34 genera of plant pathogenic or saprophytic species ...200415119856
production and characterization of somatic hybrids between upland cotton (gossypium hirsutum) and wild cotton (g. klotzschianum anderss) via electrofusion.symmetric somatic hybrid plants between gossypium hirsutum coker 201 and g. klotzschianum were obtained through electrofusion. the fusion products were cultured in km8p medium supplemented with 2.685 microm alpha-naphthaleneacetic acid and 0.465 microm kinetin, and the regenerated plants were morphologically, genetically, and cytologically characterized. nuclear-dna flow cytometric analysis revealed that the plants tested (31 of 40) had a relative dna content close to the total dna contents of t ...200415114473
modeling the impact of ozone x drought interactions on regional crop yields.the influence of soil moisture stress on crop sensitivity to o3 was evaluated for corn (zea mays l.), cotton (gossypium hirsutum l.), soybean (glycine max l. merr.), and wheat (triticum aestivum l.) grown in the united states. this assessment was accomplished by using yield forecasting models to estimate the influence of soil moisture deficits on regional yield and a previously developed model to predict moisture stress x o3 interactions. reduced crop sensitivity to o3 was predicted for those re ...198815092561
physiological and growth responses of differentially irrigated cotton to ozone.this study was conducted to determine the physiological and growth responses of cotton (gossypium hirsutum l.) to the interaction of ozonee (o3) and drought stress. cotton (cv sj-2) was grown in open-top chambers in the field at three levels of soil water and exposed to charcoal-filtered air (cf), nonfiltered air (nf), and nf x 1.25, and nf x 1.5 ambient o3 concentrations in riverside, ca, from june to october 1986. ozone reduced carbon fixation an average of 74.6% in optimally watered (ow) plot ...198815092554
effects of ddt on the growth of crop plants.the effects of ddt on the germination and growth of plants were studied using many crop species. of the species tested, oil-rich seeds of plants, such as peanut (arachis hypogaea) and mustard (brassica juncea), were more prone to ddt induced inhibition of germination and subsequent plant growth than cereals, pulses and fibre crops, like rice (oryza sativa), barley (hordeum vulgare), mung bean vigna radiata), pigeon pea (cajanus cajan) and cotton (gossypium hirsutum). studies with (14)c labelled ...198915092369
relationship between the heat tolerance of photosynthesis and the thermal stability of rubisco activase in plants from contrasting thermal environments.inhibition of net photosynthesis (pn) by moderate heat stress has been attributed to an inability of rubisco activase to maintain rubisco in an active form. to examine this proposal, the temperature response of pn, rubisco activation, chlorophyll fluorescence, and the activities of rubisco and rubisco activase were examined in species from contrasting environments. the temperature optimum of rubisco activation was 10 degrees c higher in the creosote bush (larrea tridentata) compared with the ant ...200415084731
estimate of alabama argillacea (hübner) (lepidoptera: noctuidae) development with nonlinear models.the objective of this work was to evaluate which nonlinear model [davidson (1942, 1944), stinner et al. (1974), sharpe & demichele (1977), and lactin et al. (1995)] best describes the relationship between developmental rates of the different instars and stages of alabama argillacea (hübner) (lepidoptera: noctuidae), and temperature. a. argillacea larvae were fed with cotton leaves (gossypium hirsutum l., race latifolium hutch., cultivar cnpa 7h) at constant temperatures of 20, 23, 25, 28, 30, 33 ...200315029370
wide coverage of the tetraploid cotton genome using newly developed microsatellite markers.microsatellite [simple-sequence repeat (ssr)] markers were developed and positioned on the genetic map of tetraploid cotton. three hundred and ninety-two unique microsatellite sequences, all but two containing a (ca/gt) repeat, were isolated, and the deduced primers were used to screen for polymorphism between the gossypium hirsutum and g. barbadense parents of the mapping population analyzed in our laboratory. the observed rate of polymorphism was 56%. the 204 polymorphic ssrs revealed 261 segr ...200414997301
early-season colonization patterns of the boll weevil (coleoptera: curculionidae) in central texas cotton.it is commonly believed that colonization of early-season cotton, gossypium hirsutum l., by overwintered boll weevils, anthonomus grandis grandis boheman, is concentrated on field margins. however, supporting experimental evidence is not available. in 1999 and 2000, we examined colonization patterns of overwintered boll weevils in central texas cotton on the bases of adult collections by a pneumatic sampler and hand collections of abscised infested squares. samples were taken from sites arranged ...200314994797
[genetic analysis of the duration of the vegetative period in cotton gossypium hirsutum l. with background of various nitrogen level in soil].using hayman's model of genetic analysis of the f1 hybrids from crosses between early- and late-ripening forms of cotton gossypium hirsutum l., we examined inheritance of the duration of vegetative period at various contents of nitrogen in soil. two variants of hybrid plants were studied. with the natural content of soil nitrogen, incomplete directionally dominance of earliness over late ripening with prevalence of additive gene effect of nonallelic interactions was observed. the genetic formula ...200314964832
fly ash as a liming material for cotton.a field experiment was conducted to determine the effect of fly ash from a coal combustion electric power facility on soil acidity in a cotton (gossypium hirsutum l.) field. fresh fly ash was applied to a bosket fine sandy loam (fine-loamy, mixed, thermic mollic hapludalf) soil with an initial soil ph(salt) of 4.8. the fly ash was equivalent to 42 g kg(-1) calcium carbonate with 97% passing through a 60 mesh (u.s. standard) sieve. fly ash was applied one day before cotton planting in 1999 at 0, ...201314964389
improvement of cotton fiber quality by transforming the acsa and acsb genes into gossypium hirsutum l. by means of vacuum infiltration.a novel method for the genetic transformation of cotton pollen by means of vacuum infiltration and agrobacterium-mediated transformation is reported. the acsa and acsb genes, which are involved in cellulose synthesis in acetobacter xylinum, were transferred into pollen grains of brown cotton with the aim of improving its fiber quality by incorporating useful prokaryotic features into the colored cotton plants. transformation was carried out in cotton pollen-germinating medium, and transformation ...200414740167
variation in surface chemical constituents of cotton (gossypium hirsutum) fiber as a function of maturity.modern cotton yarn production technology has made it imperative that new predictors of yarn spinning efficiency be determined. surface frictional forces play a large role in spinning efficiency, yet little is known about the chemical constituents comprising the cotton fiber surface or their respective roles in inter-fiber frictional behavior. major cotton fiber surface chemical components including pectin, wax, soluble salts, and sugars were quantified, and their respective relationships to cott ...200314690385
cotton defoliant runoff as a function of active ingredient and tillage.cotton (gossypium hirsutum l.) defoliant runoff was recently identified as an ecological risk. however, assessments are not supported by field studies. runoff potential of three defoliant active ingredients, dimethipin (2,3-dihydro-5,6-dimethyl-1,4-dithiin 1,1,4,4-tetraoxide), thidiazuron (n-phenyl-n-1,2,3-thidiazol-5-yl-urea), and tribufos (s,s,s-tributyl phosphorotrithioate) was investigated by rainfall simulation on strip (st) and conventionally tilled (ct) cotton in south central georgia. si ...200614674540
overwintering of heliothis virescens (f.) and helicoverpa zea (boddie) (lepidoptera: noctuidae) in cotton fields of northeast mississippi.following the 1995-2001 crop seasons, population densities of pupae of tobacco budworm, heliothis virescens (f.), and corn earworm, helicoverpa zea (boddie), overwintering in cotton fields, gossypium hirsutum l., in monroe county, ms, were estimated by digging a total of 43,542 m-row. densities of pupae varied greatly among years with the highest densities occurring in years with low rainfall in the late summer and early fall. the sex ratio of pupae was variable and significantly male-biased in ...200314650516
cotton fiber germin-like protein. i. molecular cloning and gene expression.the presence of cotton ( gossypium hirsutum l.) fiber transcripts coding for a germin-like protein (glp) was revealed by differential display analysis in which early stages of cotton fiber development between a wild type line, texas marker-1 (tm1) and a near isogenic mutant, naked seed (n1) were compared. transcripts of the cotton glp ( ghglp1) accumulated specifically in tm1, but did not accumulate in the mutant although the ghglp1 gene was present in both lines. the deduced protein sequence of ...200414648117
cotton-fiber germin-like protein. ii: immunolocalization, purification, and functional analysis.cotton (gossypium hirsutum l.) contains a germin-like protein (glp), ghglp1, that shows tissue-specific accumulation in fiber. the fiber glp is an oligomeric, glycosylated protein with a subunit size of approximately 25.5 kda. accumulation of ghglp1 occurs during the period of fiber elongation [4-14 days post-anthesis (dpa)]. during early phases of fiber development (2-4 dpa), ghglp1 localizes to cytoplasmic vesicles as shown by confocal immunofluorescent microscopy. in slightly older fibers (7- ...200414634817
the dna sequence of a gypsy element from gossypium hirsutum l. and characterization of gypsy elements in three gossypium species.a strategy was developed to isolate a complete gypsy-element from gossypium hirsutum l. based on the sequence of a rapd that was polymorphic in near isoganic lines of cotton that varied in leaf shape. a 5998 nt clone was isolated and its gene order and sequence confirmed it was a gypsy-type retroelement. sequences homologous to the gag portion of this clone were also found in gossypium herbaceum l. and gossypium raimondii l. a portion of the open reading frame of the integrase gene was amplified ...200314631654
volatiles released from cotton plants in response to helicoverpa zea feeding damage on cotton flower buds.feeding of helicoverpa zea larvae on cotton ( gossypium hirsutum l.) flower buds (squares) for 24 or 48 h induced the release of a number of terpenes [( e)-beta-ocimene, linalool, ( e)-beta-farnesene, ( e, e)-alpha-farnesene, ( e)-4,8-dimethyl-1,3,7-nonatriene, ( e, e)-4,8,12-trimethyl-1,3,7,11-tridecatetraene], isomeric hexenyl butyrates, 2-methylbutyrates, indole and ( z)-3-hexenyl acetate. these compounds are not released in significant amounts from undamaged squares and freshly damaged squar ...200414625774
the use of multiple alien chromosome addition aneuploids facilitates genetic linkage mapping of the gossypium g genome.primary germplasm pools represent the most accessible source of new alleles for crop improvement, but not all effective alleles are available in the primary germplasm pool, and breeders must sometimes confront the difficulties of introgressing genes from the secondary and tertiary germplasm pools in cotton by using synthetic polyploids as introgression bridges. two parental gossypium nelsonii x gossypium australe aflp genetic linkage maps were used to identify g genome chromosome-specific molecu ...200314608394
changes in oxidative stress enzymes during artificial ageing in cotton (gossypium hirsutum l.) seeds.the present study was carried out to elucidate the mechanism of seed deterioration in two cotton (gossypium hirsutum l.) cultivars (hs6 and h1098). the seeds were artificially aged at 40 +/- 1 degree c and 100% relative humidity for 4 days. in both cultivars, germinability decreased, whereas membrane deterioration, as assayed by electrical conductivity of the seed leachates, increased progressively with artificial ageing. the decrease in germinability was well correlated with increased accumulat ...200314593811
effects of drought stressed cotton, gossypium hirsutum l., on beet armyworm, spodoptera exigua (hübner), oviposition, and larval feeding preferences and growth.the beet armyworm, spodoptera exigua (hübner), has been anecdotally reported to oviposit more on drought stressed than on nonstressed cotton plants. using potted cotton plants in cages, this study demonstrated that beet armyworms deposited 3.3, 4.6, and 2.3 times more (p < or = 0.05) eggs on cotton plants that were grown on 1500, 1000, and 750 ml water/wk, respectively, than on cotton plants grown in well watered (4000 ml water/wk) soil. third instars, however, showed no preference for stressed ...200314584672
identification of ghmyb109 encoding a r2r3 myb transcription factor that expressed specifically in fiber initials and elongating fibers of cotton (gossypium hirsutum l.).cotton (gossypium hirsutum l.) fibers are derived from ovule epidermis, which are developmentally similar to arabidopsis trichome where several myb transcription factors have been shown to control their formation. however, little is known about the molecular control of cotton fiber initiation. here we isolated 55 cotton myb domain-containing sequences expressed in ovules during fiber initiation. among them, ghmyb109, encoding a r2r3 myb transcription factor of 234 amino acids, was found to be st ...200314580676
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