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immunological crossreactivity of the mycobacterium leprae cfp-10 with its homologue in mycobacterium tuberculosis.mycobacterium tuberculosis culture filtrate protein-10 (cfp-10) (rv3874) is considered a promising antigen for the immunodiagnosis of tuberculosis (tb) together with early secreted antigens of m. tuberculosis (esat-6). both esat-6 and cfp-10 are encoded by the rd1 region that is deleted from all tested m. bovis bacille calmette-guérin (bcg) strains but present in m. leprae, m. tuberculosis, m. bovis, m. kansasii, m. africanum and m. marinum. in this study, the homologue of cfp-10 in m. leprae (m ...200414723623
under-explored experimental topics related to integral mycobacterial vaccines for leprosy.many leprosy vaccine studies have utilized live or killed whole mycobacteria, such as bacille calmette-guérin, indian cancer research center (icrc) bacilli and mycobacterium w either alone or in combination with killed mycobacterium leprae. for bacille calmette-guérin, the vaccine dose is generally that which gives the largest delayed-type hypersensitivity response with minimal side effects. the doses of other integral mycobacterial vaccines appear to be arbitrarily chosen. hypotheses governing ...200314711362
[neurological manifestations of leprosy].leprosy, also known as hansen's disease, is a chronic, infectious disease caused by mycobacterium leprae. bacilli localize preferentially in the skin and peripheral nerves and have a propensity to cause nerve damage. the resulting disability has caused great suffering for victims in many countries. despite recent advances in the immunopathogenesis, epidemiology and prognostic factors of leprosy nerve damage, many aspects of the disease have remained enigmatic. the spectrum of clinical and pathol ...200314710019
leprosy: a primer for canadian physicians.leprosy is a rare but serious infectious disease caused by mycobacterium leprae. while global prevalence of the disease is decreasing, increasing rates of immigration from countries where leprosy is endemic have led to the recognition of this illness in north america. classically, leprosy presents as hypopigmented cutaneous macules along with sensory and motor peripheral neuropathies, although the clinical manifestations vary along a disease spectrum. in addition to primary infection, patients m ...200414707226
did the loss of two-component systems initiate pseudogene accumulation in mycobacterium leprae? 200414702392
a polyketide synthase catalyzes the last condensation step of mycolic acid biosynthesis in mycobacteria and related organisms.mycolic acids are major and specific constituents of the cell envelope of corynebacterineae, a suborder of bacterial species including several important human pathogens such as mycobacterium tuberculosis, mycobacterium leprae, or corynebacterium diphtheriae. these long-chain fatty acids are involved in the unusual architecture and impermeability of the cell envelope of these bacteria. the condensase, the enzyme responsible for the final condensation step in mycolic acid biosynthesis, has remaine ...200414695899
[serodiagnostic monitoring of leprosy]. 200314689796
effects of anti-endothelial cell antibodies in leprosy and malaria.as a result of damaging endothelial cells (ecs), mycobacterium leprae triggers the production of antibodies (abs). these anti-ec abs (aecas) can be divided into two types. the first type nonspecifically reacts with components of the cytosol (cy) and can be detected by enzyme-linked immunosorbent assay (elisa). the second specifically reacts with the ec membrane (mb) and requires fluorescence-activated cell sorter (facs) analysis to be detected. the presence of both types of aecas was determined ...200414688109
anti-mycobacterial immunity induced by a single injection of m. leprae hsp65-encoding plasmid dna in biodegradable microparticles.a single sub-cutaneous injection of a plasmid dna encoding a mycobacterial heat shock protein 65 (hsp65) entrapped in biodegradable poly(lactic-co-glycolic acid) microspheres produced high titers of antibodies, measured 5 months after the injection in balb/c mice. splenocytes secreted ifn-gamma and exerted an anti-bacterial effect on macrophages infected in vitro with mycobacterium tuberculosis. the results are encouraging with regard to obtaining good compliance and vaccination coverage with ca ...200314687708
nk cells modulate the cytotoxic activity generated by mycobacterium leprae-hsp65 in leprosy patients: role of il-18 and il-13.protection against intracellular pathogens such as mycobacterium leprae is critically dependent on the function of nk cells at early stages of the immune response and on th1 cells at later stages. in the present report we evaluated the role of il-18 and il-13, two cytokines that can influence nk cell activity, in the generation of m. leprae-derived hsp65-cytotoxic t lymphocytes (ctl) from peripheral blood mononuclear cells (pbmc) of leprosy patients. we demonstrated that il-18 modulates hsp65-in ...200414678270
[hansen's disease in the laboratory].a physical doctor with a phd in pathology, euzenir nunes sarno studies the immunology factors of hansen's disease, one of the oldest chronic infections and that is an exclusively human disease. staff member of an ambulatory that has become a reference on the disease in brazil with 220 to 250 new patients per year, euzenir emphasizes that the fact one cannot cultivate mycobacterium leprae brings about some everlasting questions in relation to the transmission of and the sensitivity to the disease ...200314650417
lepromatous phlebitis of the external jugular vein.mycobacterium leprae (m leprae), the causative agent of hansen's disease, is endemic in many areas of asia, sub-saharan africa, south and central america, the pacific islands, and the philippines. the spectrum of clinical disease is dependent on the patient's cell-mediated immunity and might range from localized anesthetic patches or plaques to disseminated disease. if undiagnosed, progression with damage to the involved sensory and motor nerves might occur. lepromatous vasculitis occurs most co ...200314639414
lepromatous leprosy in a heart transplant recipient.northern louisiana is not an area for indigenous cases of leprosy. limited data are available on the occurrence of leprosy in organ transplant recipients. no cases have been reported in heart transplant recipients. mr j.r. is a 68-year-old man from shreveport, louisiana. he underwent orthotopic heart transplantation in march 1996. he presented in march 2000 with a maculopapular skin rash and intermittent hand swelling for 5 months. he also complained of intermittent burning of his feet for a yea ...200314629293
a second case of multidrug-resistant mycobacterium leprae isolated from a japanese patient with relapsed lepromatous leprosy.emergence of drug resistant strains of mycobacterium leprae was reported soon after the introduction of dapsone (diamino-diphenyl sulphone, dds) for leprosy treatment (6, 10, 11). three cases of multidrug-resistant strains of m. leprae have been reported recently (2, 8, 9, 13). in order to prevent multiple drug resistant strains of m. leprae from developing, current leprosy control strategies are based on early detection of cases and treatment with multidrug therapy (mdt) as recommended by the w ...200314608820
electron microscope appearance of lepromatous footpads of nude mice [corrected].footpad lesions of 3 nude mice infected by mycobacterium leprae were studied at 9, 12, and 14 months after inoculation with light and electron microscope. the lesions were somewhat similar to those found in nodules in polar lepromatous leprosy. striated muscles rather than nerves were the preferred site of the growth of m. leprae. yet, m. leprae were identified in schwann cells and endothelial cells, singly and in clumps. m. leprae filled macrophages, and free m. leprae were found in large numbe ...200314608819
"relatively spared zones" in leprosy: a clinicopathological study of 500 patients.in this study, clinically all forms of lesions like macules, plaques, and nodules were found in all the "relatively spared zones," except groins. histopathology confirmed that the disease process was established and the acid-fast bacilli were not present as a part of bacteremic settlement. hence, it appears that practically no area on the surface of skin is immune to invasion by m. leprae. however, as the incidence of lesions and afb in these regions is relatively less, especially over axilla an ...200314608818
nodular leprosy of childhood and tuberculoid leprosy: a comparative, morphologic, immunopathologic and quantitative study of skin tissue reaction.nodular leprosy of childhood (nl) is a benign clinical variant of tuberculoid leprosy that affects breast-feeding infants and children that remained in a highly infected environment. the lesions resolve with complete healing and nl has been considered a manifestation of allergy and congenital immunity to mycobacteria leprae. we studied the tissue reaction, mycobacterial antigen frequency, and the lymphocyte subsets (cd45ro+, cd4+, cd8+, b, nk), dendritic cells (epidermal cd1a+ cells and s100+ de ...200314608817
viability and drug susceptibility testing of m. leprae using mouse footpad in 37 relapse cases of leprosy.mycobacteria leprae isolates obtained from 37 referral relapse cases of leprosy (37 skin and 10 nerve biopsy samples) received during the years 1994-2001, were tested for viability and drug sensitivity in the mouse footpad. a significant m. leprae yield in the footpads of control mice was obtained, with 32/47 (68%) isolates (from 26 cases) thus confirming viability. of the 28 isolates successfully drug tested, 6 (21%) were resistant to one or more drugs. all except one, were multidrug treated ca ...200314608816
[a case suspected multibacillary leprosy].the presence of acid-fast bacilli in the lesion and its positive culture usually provide the diagnosis in cutaneous mycobacteriosis. but in diagnosis of leprosy, characteristic clinico-histopathological findings are so important as the demonstration of the organism, because of the failure to grow mycobacterium leprae in vitro. it is classified in various forms along a spectrum of infectious and immunological symptoms supported by the pathogen-host relationship. the patient was a japanese woman 6 ...200314598642
[a case of paucibacillary leprosy].we report a case of leprosy. a 31-old-phillippine female present with 5 month history of an erythematous plaqe on her right neck. examination showed 4 erythematous plaques with her. a histological examination of the skin lesion of hir thigh revealed granulomas around nerve and skin appendages which consist of lymphocytes, epidermoid cells and giant cells. although no acid fast bacilli were not demonstrated by skin biopsy with ziehl-neelsen staining, the mycobacterium leprae-specific dna was dete ...200314598636
nasal carriage of mycobacterium leprae dna in healthy individuals in lega robi village, ethiopia.the number of registered leprosy patients world-wide has decreased dramatically after extensive application of who recommended multiple drug therapy (mdt). the annual number of new cases has, however, been almost unchanged in several populations, indicating that the infection is still present at community level. nasal carriage of mycobacterium leprae dna was studied in lega robi village in ethiopia. mdt had been applied for more than ten years, and 718 residents over 5 years old were eligible fo ...200314596524
advances in the diagnosis and treatment of leprosy.leprosy is a chronic infectious disease caused by mycobacterium leprae that mainly affects the skin and peripheral nerves. over recent years, many important advances have been made in developing molecular diagnostics, in identifying highly effective drugs and designing multidrug regimens for treatment, and in unravelling the genomic structure and functions of the leprosy bacillus. using the new information about specific sequences of m. leprae, several gene probes and gene amplification systems ...200214585155
comparison between anti-pgl-i serology and mitsuda reaction: clinical reading, microscopic findings and immunohistochemical analysis.the lepromin test, serum igm antibodies against mycobacterium leprae and in situ observations of t cell subsets in biopsies of mitsuda reaction using monoclonal antibodies were performed on 44 untreated leprosy patients belonging to various classifications of the disease. the mitsuda reaction was accessed clinically and histologically after 28 days. clinical reading and histological analysis of mitsuda reaction showed good agreement. the high positivity in clinical reading correlated with compac ...200314577472
thalidomide does not modify the ability of cells in leprosy patients to incorporate [3h]-thymidine when incubated with m. leprae antigens.the immune response in reversal reaction, (rr) and in erythema nodosum leprosum (enl) is characterized in vitro by an enhancement in lymphocyte blast transformation against m. leprae. as thalidomide is an effective treatment for enl, this study assessed the effect of this drug on these phenomena. mononuclear cells from patients attending the clinic at alert and from healthy staff were cultured for 5 days with integral m. leprae (iml), or a modified dharmendra antigen (dhar), or ppd from m. tuber ...200314577465
serology: recent developments, strengths, limitations and prospects: a state of the art overview.specific antibodies can be used as a surrogate marker for bacterial load in leprosy. tests to detect antibodies can be used for (i) the classification of patients for treatment purposes [most multibacillary (mb) patients are seropositive, most paucibacillary (pb) patients are not], (ii) the prediction of an increased risk of relapse and (iii) the identification of contacts having an increased risk of developing leprosy. with the advent of fast, robust and easy to perform serological tests such a ...200314577464
a tetramer-octamer equilibrium in mycobacterium leprae and escherichia coli ruva by analytical ultracentrifugation.in the context of the bacterial ruvabc system, ruva protein binds to and is involved in the subsequent processing of a four-way dna structure called holliday junction that is formed during homologous recombination. four crystal structures of ruva from escherichia coli (ecoruva) showed that it was tetrameric, while neutron scattering and two other crystal structures for ruva from mycobacterium leprae (mleruva) and ecoruva showed that it was an octamer. to clarify this discrepancy, sedimentation e ...200314568529
reversal of t cell anergy in leprosy patients: in vitro presentation with mycobacterium leprae antigens using murabutide and trat peptide in liposomal delivery.mycobacterium leprae, the causative agent of leprosy resides and multiplies within the host monocytes and macrophages, thereby evading host immune system. cell-mediated immune response (cmi) plays a vital role as evidenced from the high cmi in bt/tt (borderline and tuberculoid) patients and conversely low in bl/ll (borderline and lepromatous) patients. in the present study, an attempt was made to immunomodulate the anergized t cells of lepromatous leprosy patients by presenting the mycobacterial ...200314555284
dna sequences of mycobacterium leprae recovered from ancient bones. 200314553941
the secret lives of the pathogenic mycobacteria.pathogenic mycobacteria, including the causative agents of tuberculosis and leprosy, are responsible for considerable morbidity and mortality worldwide. a hallmark of these pathogens is their tendency to establish chronic infections that produce similar pathologies in a variety of hosts. during infection, mycobacteria reside in macrophages and induce the formation of granulomas, organized immune complexes of differentiated macrophages, lymphocytes, and other cells. this review summarizes our und ...200314527294
purification of mycobacterium leprae rna for gene expression analysis from leprosy biopsy specimens.gene expression analysis in mycobacterium leprae, an obligate intracellular pathogen and the etiologic agent of leprosy, has been hampered by the lack of an efficient method to purify rna from leprosy lesions. therefore to date, transcripts for only a few genes have been identified. we report the use of a single-tube homogenization/rna extraction method that produces enough rna to study the expression of 30 genes from a single skin biopsy specimen of a multibacillary leprosy patient and demonstr ...200314513559
in situ type 1 cytokine gene expression and mechanisms associated with early leprosy progression.we explored the prognostic value of in situ cytokine patterns in 39 patients with single-skin-lesion paucibacillary leprosy before single-dose therapy, with 3 years of follow-up. interferon (ifn)-gamma, interleukin (il)-12, il-10, il-4, tumor necrosis factor (tnf)-alpha, and macrophage inflammatory protein (mip)-1alpha mrna was quantified in skin biopsy samples at diagnosis, and mycobacterium leprae dna was detected in 51.4% of cases. type 1 immunity predominance with measurable ifn-gamma and un ...200314513423
leprosy: current diagnostic and treatment approaches.leprosy remains an important problem globally and leprosy patients may present to physicians outside leprosy endemic areas. we review the recent biological and clinical advances in leprosy.200314501994
a granulomatous lesion of mouse embryomas produced by mycobacterium leprae. 196214492807
[studies on the biological characteristics of mycobacterium leprae murium. 3. studies on the preservation of m. leprae murium by means of lyophilization]. 196214478152
[studies on the biological characteristics of mycobacterium leprae murium. 1. study on the effect of the ph of the medium on the maintenance of virulence of m. leprae murium in vitro]. 196214478151
[use of the shading technic for the study of "mycobacterium leprae"]. 195914420288
[essay on the growth of mycobacterium leprae in cultures of renal cells of rhesus monkeys]. 195914413433
enumeration of mycobacterium leprae for the standardization of lepromin. 195914399510
[discovery of a cultivable and inoculable hansen's bacillus]. 195514381813
[the mycobacterium marianum is not mycobacterium leprae]. 195414377651
[histological studies of the changes in specific leprotic tissues and the effects of isonicotinic acid hydrazid on mycobacterium leprae in nodular leprosy]. 195514374896
[comparative study of lesions caused by suspensions of mycobacterium leprae and mycobacterium tuberculosis injected intradermally into guinea pigs previously vaccinated with bcg]. 195414372261
[isolation and experimental study of 2 new strains of acid-alcohol-fast bacteria from the nasal mucus of lepers]. 195414369698
[a case of nodular erythema caused by hansen's bacillus; connections between this erythema and "erythema nodosum leprosum"]. 195414364150
some new bacteriological aspects of leprosy. iv. identification of the evolutionary forms of the etiological agent of leprosy in human lesions. 195414363027
some new bacteriological aspects of leprosy. ii. culture of the etiological agent of human leprosy by inoculation of liquid and solid media; yeastlike shape. 195414363025
some new bacteriological aspects of leprosy. i. culture of the etiological agent of human leprosy by the glass slide culture method; mycobacterium shape. 195414363024
the leprosy bacillus and the host reaction to it. 195514355052
[behavior of various species of mycobacteria following injection into mouse foot-pad]. 196514336051
a new technique for detecting mycobacterium leprae and the problem of infectiousness of tuberculoid leprosy. 196514333144
experiences with mouse foot pad inoculation of leprosy bacilli originating from the congo. 196514319172
[treatment of experimental infection with mycobacterium leprae in mice]. 196514319165
staining mycobacterium leprae in paraffin sections by the gomori methenamine-silver method. 196514305778
effects of physical treatments on the infectivity of myc. lepraemurium. 196414278287
[contribution to the study of the association of leprosy and gravido-puerperal states. apropos of 86 cases]. 196414265069
multiplication of mycobacterium leprae in the mouse footpad. 196414261295
immune reactions of the guinea-pig to m. leprae. 196414261291
vaccination against experimental infection with mycobacterium leprae. 196514261022
mycobacterium leprae in mice: minimal infectious dose, relationship between staining quality and infectivity, and effect of cortisone.shepard, charles c. (communicable disease center, u.s. public health service, atlanta, ga.), and dorothy h. mcrae. mycobacterium leprae in mice: minimal infectious dose, relationship between staining quality and infectivity, and effect of cortisone. j. bacteriol. 89:365-372. 1965.-the minimal infectious dose of mycobacterium leprae in mouse foot pads was found to be on the order of 10 solidly staining bacilli. in a titration experiment, the actual number found was 3.4 to 34 solid bacilli, and th ...196514255702
[immunologic classification of leprosy]. 196414250520
hansen's first observation and publication concerning the bacillus of leprosy. 196414231935
first observation of the leprosy bacillus. 196414227407
bacterial genetics. 196414227406
infections produced in hamsters with the human leprosy bacillus: a critique of recent studies. 196414227405
inoculations of m. leprae in reptiles. 196414227400
activity of antituberculosis drugs against mycobacterium leprae. 196414227399
leprosy. 196414224665
evaluation of the tubercle phosphatide kaolin agglutination test in tuberculosis. 196414215784
[multiplication and behavior of hansen's bacillus in tissue cultures. preliminary note]. 196414211546
experimental inoculation of human leprosy in laboratory animals. 3. 196414205618
a study of malachite green staining of leprosy bacilli. 196414203325
a guide to the counting of mycobacteria in clinical and experimental materials. 196414203322
effect of ionizing radiations on the rate of evolution of murine leprosy. 196414203319
immunologic studies with mycobacterium leprae and an acid-fast mycobacterium cultivated from human leprosy nodules. 196414203318
sulphone resistance in leprosy. 196414201653
[lepromatous leprosy produced by the inoculation of mycobacterium leprae in to the footpads of rats fed prooxidant diets]. 196414201325
sulphone resistance in leprosy. 196414197176
attempts at growth of mycobacterium leprae in footpads of mice and guinea-pigs. 196414192043
resistance of antihistamine-treated guinea-pigs against infection with mycobacterium leprae and m. lepraemurium. 196414192036
field method for concentrating mycobacterium leprae in skin biopsy specimens. 196414192035
mycobacterial disease in man and animals. studies on leprosy bacilli in man and animals. 196414190866
sulphone resistance in leprosy. an experimental and clinical study. 196414188912
capreomycin: activity against experimental infection with mycobacterium leprae. 196414186464
studies on suitable media for the cultivation of an acid fast mycobacterium isolated from human lepromatous leprosy. 196414175600
the effect of vaccination by atypical mycobacteria in experimental murine leprosy. 196414175599
[elements of experimental leprology]. 196314175546
histochemical changes in the tissues of different types of leprous lesions. 196414168133
electron microscopic study of borderline leprosy. 196314166266
survey of recent leprosy research. 196414155844
sarcoidosis or leprosy? 196414155117
[multiplication of mycobacterium leprae; possibility of sensitivity testing]. 196414152230
[changes in the level of protein fractions in vaccinated and tuberculosis-infected guinea pigs]. 196414147729
mycobacterium leprae: a house divided? 196414145972
metabolic pathway and its relationship to the biological activity of some tuberculostatic and leprostatic agents. 196414143116
limited multiplication of acid-fast bacilli in the foot-pads of mice inoculated with mycobacterium leprae. 196414140757
biosynthesis of ergothioneine and hercynine by mycobacteria.genghof, dorothy s. (yeshiva university, new york, n.y.), and olga van damme. biosynthesis of ergothioneine and hercynine by mycobacteria. j. bacteriol. 87:852-862. 1964.-ergothioneine and hercynine were found to be synthesized by a wide variety of mycobacteria grown in chemically defined media free from these compounds. the cultures examined included 53 recently isolated and laboratory strains of mycobacterium tuberculosis, 26 "unclassified" mycobacteria (runyon groups i to iv), and representat ...196414137624
the concentration of m. leprae in currently available lepromins. 196314132394
attempts to cultivate mycobacterium leprae murium. ten years work with negative results. 196314132393
tuberculosis and leprosy. 196414131732
recent advances in our knowledge of mycobacterium leprae. 196414128910
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