Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| controlled comparison of original vented aerobic fan medium with new nonvented bact/alert fa medium for culturing blood. | to evaluate the performance of bact/alert fa (fa) medium, a new aerobic bact/alert fan (fan) medium (organon teknika corporation, durham, n.c.) that does not require the added cost and inconvenience of a venting unit, we inoculated blood specimens from adult patients with suspected sepsis into an original fan aerobic culture bottle and an fa bottle. of 7,745 blood culture sets containing both bottles, 5,256 (68%) met the criteria for adequacy of filling. a total of 466 isolates judged to represe ... | 2001 | 11376041 |
| study of the effects of temperature, ph and yeast extract on growth and exopolysaccharides production by propionibacterium acidi-propionici on milk microfiltrate using a response surface methodology. | to study the effects of temperature, ph and yeast extract (ye) concentration on growth and exopolysaccharide (eps) production by propionibacterium acidi-propionici dsm 4900 cultivated on milk microfiltrate. | 2001 | 11348440 |
| exopolysaccharide production by propionibacterium acidi-propionici on milk microfiltrate. | the aims of this work were to evaluate growth and exopolysaccharide (eps) production properties of propionibacterium acidi-propionici dsm 4900 on milk permeate. | 2001 | 11348439 |
| utilisation of tomato pomace as a substrate for the production of vitamin b12--a preliminary appraisal. | the cellulose fraction in tomato pomace was hydrolysed using trichoderma reesei, and the resultant sugars were fermented with propionibacterium shermanii to produce vitamin b12. a multifactorial experiment revealed that aeration of the culture of t. reesei gave substantial improvements in cellulase activity as did higher concentrations of available nitrogen, but a rapid drop in ph appeared to inhibit extensive hydrolysis; after 14 days, the maximum level of cellulose degradation was only 34.4% o ... | 2001 | 11341680 |
| role of monocyte chemoattractant protein-1 in propionibacterium acnes-induced pulmonary granulomatosis. | the inflammatory process in granulomatous disorders such as sarcoidosis is mainly the consequence of delayed hypersensitivity induced by causative antigens. propionibacterial dna was isolated recently by pcr from human sarcoidosis tissue. hence, we developed a model using sensitized rabbits for t cell-mediated pulmonary granulomatosis induced by propionibacterium acnes (p. acnes) and investigated the role of monocyte chemoattractant protein-1 (mcp-1) in the pathogenesis of the granuloma formatio ... | 2001 | 11340674 |
| [extracellular protein of propionic acid bacteria inhibits induced mutation in strains of salmonella typhimurium]. | a culture of propionic acid bacteria grown in a glucose-containing minimal medium, as well as the culture liquid and logarithmic-phase cells obtained from this culture, were found to inhibit the base pair substitution mutations induced by 4-nitroquinoline n-oxide, n-methyl-n'-nitro-n-nitrosoguanidine, and sodium azide and the frameshift mutations induced by 9-aminoacridine. the antimutagenic activity of the culture liquid (cl) was presumably due to the presence of an extracellular thermolabile p ... | 2001 | 11338834 |
| propionibacterium acnes as a cause of visually significant corneal ulcers. | 2001 | 11333339 | |
| 16s rdna-based identification of bacteria from conjunctival swabs by pcr and dgge fingerprinting. | establishment of a new molecular biology technique for the identification of multiple bacteria from the ocular environment, which can be applied supplementarily to cultivation in cases of severe bacterial infections. | 2001 | 11328723 |
| digital fluorescence as a parameter of propionibacterium acnes suppression needs assessment. | 2001 | 11328389 | |
| expression, crystallization and preliminary characterization of methylmalonyl coenzyme a epimerase from propionibacterium shermanii. | methylmalonyl-coa epimerase (mmce) is an enzyme that interconverts the r and s epimers of methylmalonyl-coa in the pathway that links propionyl-coa with succinyl-coa. this is used for both biosynthetic and degradative processes, including the breakdown of odd-numbered fatty acids and some amino acids. the enzyme has been expressed in escherichia coli both as the native enzyme and as its selenomethionine (semet) derivative. crystals of both forms have been obtained by vapour diffusion using monom ... | 2001 | 11320311 |
| detection of the bacteriocin propionicin plg-1 with polyvalent anti-plg-1 antiserum. | polyclonal antibodies against the bacteriocin propionicin plg-1 were produced in rabbits at high titer (256,000 to 512,000, as determined by indirect enzyme-linked immunosorbent assay [elisa]). anti-plg-1 antiserum neutralized the antimicrobial activity of plg-1 preparations in a well diffusion assay. cross-reacting protein was detected using an indirect elisa of the culture supernatant from a fed-batch fermentation of the producer strain propionibacterium thoenii p127 within the first 24 h of i ... | 2001 | 11319106 |
| changes in protein synthesis and morphology during acid adaptation of propionibacterium freudenreichii. | survival of bacteria in changing environments depends on their ability to adapt to abiotic stresses. microorganisms used in food technology face acid stress during fermentation processes. similarly, probiotic bacteria have to survive acid stress imposed within the stomach in order to reach the intestine and play a beneficial role. propionibacteria are used both as cheese starters and as probiotics in human alimentation. adaptation to low ph thus constitutes a limit to their efficacy. acid stress ... | 2001 | 11319077 |
| evaluation of an automated culture system for detecting bacterial contamination of platelets: an analysis with 15 contaminating organisms. | approximately 1 in 2000 platelet components are bacterially contaminated. the time to detection of 15 seeded organisms in platelets recovered from an automated culture system was studied. | 2001 | 11316897 |
| antigen-specific t(h)1 cells as direct effectors of propionibacterium acnes-primed lipopolysaccharide-induced hepatic injury. | t(h)1 cells are cytotoxic effector cells that utilize fas ligand (fasl) and tumor necrosis factor. the physiological roles of cytotoxic t(h)1 cells are considered to be immunoregulation by eliminating autoreactive lymphocytes or hyper-activated foreign antigen-specific lymphocytes. their pathological roles, however, remain to be clarified. to investigate whether t(h)1 cells can destroy organs, we generated a propionibacterium acnes-specific t(h)1 clone from c57bl/6 mice and tested whether the cl ... | 2001 | 11312248 |
| antimicrobial effect of acidified nitrite on dermatophyte fungi, candida and bacterial skin pathogens. | nitric oxide is generated from sweat nitrite in the acidic environment of the skin surface and is thought to contribute to protection against infection. this study examined the sensitivity of trichophyton mentagrophytes, t. rubrum, candida albicans, streptococcus pyogenes, staphylococcus aureus and propionibacterium acnes to acidified nitrite. | 2001 | 11309079 |
| activation of clindamycin phosphate by human skin. | to investigate the relative antimicrobial activity of clindamycin phosphate (cp) and clindamycin (cly) and to examine the effect of skin homogenates on the activity of cp. | 2001 | 11309066 |
| postoperative proprionibacterium acnes endophthalmitis. | 2001 | 11297461 | |
| postoperative proprionobacterium acnes endophthalmitis. | 2001 | 11297458 | |
| the role of laparoscopic biopsies in lumbar spondylodiscitis. | the infection of an intervertebral disk is a serious condition. the diagnosis often is elusive and difficult to make. it is imperative to have appropriate microbiologic specimens before the initiation of treatment. we report the case of a 51-year-old woman with lumbar spondylodiscitis caused by infection after the placement of an epidural catheter for postoperative analgesia. a spinal magnetic resonance imaging (mri) scan confirmed the diagnosis, but computed tomography (ct)-guided fine-needle b ... | 2000 | 11285529 |
| microbial analysis of bone collected during implant surgery: a clinical and laboratory study. | dental implant surgery produces bone debris which can be used to correct bone defects in the "simultaneous-augmentation" technique. however, this debris is potentially contaminated with oral bacteria. therefore, this study examined bone debris collected during dental implant surgery in order 1) to identify the microbial contaminants and 2) to compare the effects of two different aspiration protocols on the levels of microbial contamination. twenty-four partially dentate patients were randomly al ... | 2001 | 11251657 |
| phenotypic and genotypic characterization of antibiotic-resistant propionibacterium acnes isolated from acne patients attending dermatology clinics in europe, the u.s.a., japan and australia. | propionibacterium acnes is the target of antimicrobial treatments for acne vulgaris. acquired resistance to erythromycin, clindamycin and tetracyclines has been reported in strains from diverse geographical loci, but the molecular basis of resistance, via mutations in genes encoding 23s and 16s rrna, respectively, has so far only been elucidated for isolates from the u.k. | 2001 | 11251569 |
| killing of cutaneous microbial species by photodynamic therapy. | photodynamic therapy (pdt) utilizes photosensitizers and light. whereas pdt use in cancer treatment has been widely accepted, antimicrobial pdt (apdt) is still in its early stages of development. | 2001 | 11251558 |
| antibiotics in acne: time for a rethink. | 2001 | 11251550 | |
| antibiotic sensitivity of propionibacterium acnes isolates from patients with acne vulgaris in a tertiary dermatological referral centre in singapore. | to study the minimum inhibitory concentrations (mics) of propionibacterium acnes (p. acnes) isolates to selected antibiotics from patients with acne vulgaris in singapore and determine if resistance increases with prolonged use of antibiotics. | 2001 | 11242619 |
| cytokine expression in the mouse brain in response to immune activation by corynebacterium parvum. | cytokine expression in the brain has been suggested to mediate various sickness behaviors. here we report that intraperitoneal injection of a corynebacterium parvum antigen in c57bl/6 mice was followed by prolonged upregulation of cytokines in the cerebral cortex and subcortical structures in a time course that coincided with reduced spontaneous running activity. | 2001 | 11238237 |
| metabolic biotinylation of secreted and cell surface proteins from mammalian cells. | due to its strength and specificity, the interaction between avidin and biotin has been used in a variety of medical and scientific applications ranging from drug targeting to immunohistochemistry. to maximize the application of this technology in mammalian systems, we recently demonstrated the ability to metabolically biotinylate tagged proteins in mammalian cells using the endogenous biotin ligase enzymes of the mammalian cell. this technology allows site-specific biotinylation without any exo ... | 2001 | 11237761 |
| are 2 combined antimicrobial mechanisms better than 1 for the treatment of acne vulgaris? clinical and antimicrobial results of a topical combination product containing 1% clindamycin and 5% benzoyl peroxide. introduction. | acne vulgaris is the most common chronic skin condition seen by dermatologists. available topical therapies include comedolytic agents such as tretinoin, adapalene, azelaic acid, tazarotene, and salicylic acid; bactericidal agents such as benzoyl peroxide; antibiotics such as clindamycin, erythromycin, and tetracycline; and anti-inflammatory agents such as sodium sulfacetamide and metronidazole. therapeutic failure with some antibiotic regimens due to the presence or development of resistant str ... | 2001 | 11236213 |
| potential role for a new combination topical therapy in treating mild to moderate acne vulgaris. | although a variety of established topical therapies are available to treat acne, treatment success is not always achieved with current preparations. moreover, many patients find the formulations less than fully acceptable for various reasons. a new gel that stably combines 5% benzoyl peroxide and 1% clindamycin has become available to treat mild to moderately severe inflammatory acne. there are several benefits associated with this new topical combination product: cosmetic and aesthetic acceptab ... | 2001 | 11236212 |
| the development of antibiotic resistance in propionibacterium acnes. | two separate studies evaluated the ability of a combination topical gel consisting of 5% benzoyl peroxide and 1% clindamycin to reduce facial propionibacterium acnes counts in vivo and to decrease the development of resistant organisms. in the first study, the combination gel was compared with 3 topical formulations of 1% clindamycin phosphate (gel, lotion, and solution) in 80 individuals. after only 1 week of treatment, a 99.7% reduction from baseline in facial p acnes count was obtained with t ... | 2001 | 11236211 |
| amniotic fluid microflora in asymptomatic women at mid-gestation. | the presence and composition of amniotic fluid (af) microflora, as well as af glucose concentration and white blood cell (wbc) count, were investigated in 22 consecutive asymptomatic women with intact membranes at mid-gestation. af was retrieved by trans-abdominal amniocentesis. three of the 22 women (13.6%) had microorganisms in their af: chlamydia trachomatis in 2 and both corynebacterium group absolute nonfermenter (anf) group and propionibacterium spp. in 1. no differences were found in clin ... | 2001 | 11234981 |
| [propionibacterium acnes spondylodiscitis: a case report]. | 2001 | 11234683 | |
| molecular identification of bacterial 16s ribosomal rna gene in liver tissue of primary biliary cirrhosis: is propionibacterium acnes involved in granuloma formation? | the etiopathogenesis of primary biliary cirrhosis (pbc) remains speculative. epithelioid granulomas are often found in the vicinity of damaged interlobular bile ducts in pbc, raising the possibility of a reaction to microbial materials. in this study, we tried to detect and identify bacterial dna within granulomatous lesions in pbc. using liver sections from 9 patients with pbc and 13 control livers, granuloma in portal tracts, portal tracts without granuloma, and adjacent hepatic parenchyma wer ... | 2001 | 11230731 |
| [antilysozyme activity of anaerobic bacteria from fecal microflora in man]. | for the first time anaerobic bacteria of the fecal microflora in man have been found be capable of inactivating lysozyme. the presence of this antilysozyme sign has been noted in both gram-positive anaerobes (bifidobacterium, propionibacterium, eubacterium, actinomyces israelii) and in gram-negative anaerobes (bacteroids, prevotella melaninogenica). the expression of antilysozyme activity in the anaerobes under study has been determined. the possible biological role of this sign of the indigenou ... | 2000 | 11220957 |
| correlation between microbiology and previous sinus surgery in patients with chronic maxillary sinusitis. | aspirates of 108 chronically inflamed maxillary sinuses were processed for aerobic and anaerobic bacteria. there were 295 bacterial isolates: 109 aerobic and facultative, and 186 anaerobic. the predominant aerobic isolates were staphylococcus aureus (17 isolates), alpha-hemolytic streptococci (14), pseudomonas aeruginosa (12), moraxella catarrhalis (10), and haemophilus spp (8). the predominant anaerobes were peptostreptococcus spp (61), prevotella spp (45), fusobacterium spp (15), and propionib ... | 2001 | 11219522 |
| bacterial populations on 30-night extended wear silicone hydrogel lenses. | ocular infection and inflammation during hydrogel lens extended wear is often associated with colonization of the lenses with bacteria. this study compares colonization of a high dk silicone hydrogel contact lens (lotrafilcon a) worn on a 30-night extended wear basis to a low dk hema-based lens (etafilcon a) worn on a 6-night extended wear schedule. | 2001 | 11215603 |
| [bacterial contamination of platelet concentrates by propionibacterium acnes]. | in this study, three incidents of platelet contamination by proprionibacterium acnes and an investigation of the transfusion process have been reported, which occurred at the nord-pas-de-calais blood center over a period of several months. p. acnes is a bacterium that is present in the cutaneous flora; it does not produce any toxin, and is rarely considered as a pathogenic agent; its occurrence is widespread, in particular in those regions that are rich in sebum (face, back, scalp), and it is ex ... | 2000 | 11204839 |
| assessment of periradicular microbiota by dna-dna hybridization. | in the present study the "checkerboard" dna-dna hybridization technique was used to identify bacteria in periapical endodontic lesions of asymptomatic teeth. thirty-four patients with root-filled teeth and apical periodontitis were divided into two groups, each containing 17 patients. in group 1, a marginal incision was performed during surgery to expose the lesion, and in group 2, a submarginal incision was applied. the gingiva and mucosa were swabbed with an 0.2% chlorhexidine gluconate soluti ... | 2000 | 11202881 |
| microbiology of serous otitis media in children: correlation with age and length of effusion. | the purpose of this study was to correlate the microbiology of serous otitis media in children with the duration of the condition and the patient's age. aspirates of serous ear fluids from 114 children were examined for aerobic and anaerobic bacteria. bacterial growth was noted in 47 patients (41%). aerobic organisms only were recovered in 27 aspirates (57% of the culture-positive aspirates); anaerobic bacteria only in 7 (15%); and mixed aerobic and anaerobic bacteria in 13 (28%). a total of 83 ... | 2001 | 11201816 |
| the selenoorganic compound ebselen suppresses liver injury induced by propionibacterium acnes and lipopolysaccharide in rats. | ebselen (2-phenyl-1,2-benzoisoselenazol-3[2h]-one) is a selenoorganic compound containing selenium that has various pharmacological effects, including anti-inflammatory and antioxidant activity. kupffer cells, residual hepatic macrophages, play an important role in the development of liver injury by producing free radicals and cytokines. the aim of this study is to evaluate whether ebselen suppresses macrophage-associated liver injury in rats. in vivo, we examined the effects of ebselen on liver ... | 2001 | 11179515 |
| crystallization and preliminary x-ray analysis of the 12s central subunit of transcarboxylase from propionibacterium shermanii. | the hexameric 12s central subunit of transcarboxylase has been crystallized in both free and substrate-bound forms. the apo crystals belong to the cubic space group p4(2)32, with unit-cell parameters a = b = c = 188.5 a, and diffract to 3.5 a resolution. crystals of two substrate-bound complexes, 12s with methylmalonyl coa and 12s with malonyl coa, are isomorphous and belong to space group c2, with unit-cell parameters a = 115.5, b = 201.4, c = 146.9 a, beta = 102.7 degrees. these crystals diffr ... | 2001 | 11173475 |
| a rapid procedure for isolating chromosomal dna from lactobacillus species and other gram-positive bacteria. | this study describes a rapid procedure for the isolation of genomic dna from various gram-positive bacteria. species tested included lactobacillus delbrueckii subsp. lactis atcc 4797, lact. acidophilus n2, staphylococcus aureus, staph. epidermidis, propionibacterium jensenii p126, bacillus pumilus and enterococcus faecalis. our technique for chromosomal dna isolation circumvents the need for phenol-chloroform extractions and caesium chloride gradients. isolated dna is consistently greater than 2 ... | 2001 | 11169042 |
| molecular and structural analysis of two novel mutations in a patient with mut(-) methylmalonyl-coa deficiency. | inherited defects in the gene encoding the methylmalonyl-coa mutase (mcm) result in the mut forms of methylmalonic aciduria (mma). twelve mutations have been identified associated with the mut(-) phenotype. we report two novel mutations (k621n and d156n) in a compound heterozygote mut(-) patient. these two mutations and three previously published ones (h627n, a191e, y231n) were mapped onto a three-dimensional homology model of the human mcm constructed from the crystal structure of the propionib ... | 2001 | 11161845 |
| blockade of secondary lymphoid tissue chemokine exacerbates propionibacterium acnes-induced acute lung inflammation. | chemokine-chemokine receptor interaction plays an essential role in leukocyte/dendritic cell (dc) trafficking in inflammation and immune responses. we investigated the pathophysiological roles of secondary lymphoid tissue chemokine (slc; ccl21) and macrophage inflammatory protein-2 (mip-2) in the development of acute pulmonary inflammation induced by an intratracheal injection of propionibacterium acnes in mice. immunohistochemical studies revealed that slc was constitutively expressed in the pe ... | 2001 | 11160258 |
| efficient transformation system for propionibacterium freudenreichii based on a novel vector. | a 3.6-kb endogenous plasmid was isolated from a propionibacterium freudenreichii strain and sequenced completely. based on homologies with plasmids from other bacteria, notably a plasmid from mycobacterium, a region harboring putative replicative functions was defined. outside this region two restriction enzyme recognition sites were used for insertion of an escherichia coli-specific replicon and an erythromycin resistance gene for selection in propionibacterium. hybrid vectors obtained in this ... | 2001 | 11157209 |
| the role of laparoscopic biopsies in lumbar spondylodiscitis. | infection of an intervertebral disk is a serious condition. diagnosis often is elusive and difficult. it is imperative to obtain appropriate microbiological specimens before initiation of treatment. the authors describe a 51-year-old woman with lumbar spondylodiscitis that was because of infection after the placement of an epidural catheter for postoperative analgesia. a spinal magnetic resonance imaging confirmed the diagnosis, but computed tomography-guided fine needle biopsy did not provide a ... | 2000 | 11147923 |
| chronic destructive oligoarthritis associated with propionibacterium acnes in a female patient with acne vulgaris: septic-reactive arthritis? | propionibacterium acnes is an anaerobic bacillus implicated in certain chronic arthritides. this report describes an hla-b27+ 17-year-old woman with acne vulgaris who presented with rapidly destructive arthritis in the left shoulder as well as an evolving left subclavicular adenopathy. one year later, arthritis was detected in the left knee; the inflammatory synovial fluid was sterile. growth of p acnes was observed in cultures of the shoulder synovium and lymph nodes, but polymerase chain react ... | 2000 | 11145044 |
| [azithromycin: an anti-inflammatory effect in chronic recurrent multifocal osteomyelitis? a preliminary report]. | in this preliminary communication we report our experience with azithromycin in patients with chronic recurring multifocal osteomyelitis (crmo). seven out of 13 patients, mainly teenager, showed a fast clinical improvement after they were started on azithromycin. the immediate therapeutic effect of azithromycin in patients with crmo was surprising and lead us to the hypothesis that azithromycin could have an antiphlogistic in addition to it's antibiotic effect in this disease setting. in patient ... | 2000 | 11142932 |
| regulation by chemokines of circulating dendritic cell precursors, and the formation of portal tract-associated lymphoid tissue, in a granulomatous liver disease. | we have studied the recruitment and roles of distinct dendritic cell (dc) precursors from the circulation into propionibacterium acnes-induced granulomas in mouse liver. during infection, f4/80(-)b220(-)cd11c(+) dc precursors appeared in the circulation, migrated into the perisinusoidal space, and matured within newly formed granulomas. recruited dcs later migrated to the portal area to interact with t cells in what we term "portal tract-associated lymphoid tissue" (palt). macrophage inflammator ... | 2001 | 11136819 |
| first evidence of lysogeny in propionibacterium freudenreichii subsp. shermanii. | dairy propionic acid bacteria, particularly the species propionibacterium freudenreichii, play a major role in the ripening of swiss type cheese. isometric and filamentous bacteriophages infecting p. freudenreichii have previously been isolated from cheese. in order to determine the origin of these bacteriophages, lysogeny of p. freudenreichii was determined by isometric bacteriophage type analysis. the genomic dna of 76 strains were hybridized with the dna of nine bacteriophages isolated from s ... | 2001 | 11133450 |
| effect of glycine betaine on osmoadaptation of propionibacterium acidipropionici cultivated in elevated osmolarities. | the sensitivity of industrial strains acetobacter aceti, gluconobacter frateurii, and propionibacterium acidipropionici to osmotic stress was studied. growth of a. aceti and g. frateurii was totally inhibited at 0.4 m nacl concentration, but p. acidipropionici was able to grow on a medium containing 1.2 m nacl. addition of glycine betaine to the medium had no detectable osmoprotective effect on a. aceti and g. frateurii cultivations in elevated nacl concentrations, but it enabled cells of p. aci ... | 2000 | 11131399 |
| comparison of the effectiveness of some pleural sclerosing agents used for control of effusions in malignant pleural mesothelioma: a review of 117 cases. | management of malignant pleural mesothelioma (mpm) has been an important clinical issue regardless of the treatment modality employed. we aimed to investigate the efficacy of oxytetracycline (ot), corynebacterium parvum (cp), and nitrogen mustard (nm) in the management of pleural effusion associated with mpm. | 2000 | 11124644 |
| inherited il-12 unresponsiveness contributes to the high lps resistance of the lps(d) c57bl/10sccr mouse. | lps(d) mouse strains are characterized by the presence of a defective lps/tlr4 gene that make them refractory to the biological activity of lps. one of the mouse strains commonly used to study lps defects is the c57bl/10sccr (cr) strain. however, unlike other lps(d) strains, the cr strain also has a heavily impaired ifn-gamma response to micro-organisms. as a consequence, unlike other lps(d) mouse strains, they do not acquire a partial lps susceptibility when treated with sensitizing bacteria. b ... | 2001 | 11123338 |
| comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria. | the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ... | 2001 | 11120995 |
| propionibacterium granulosum: a rare cause of endocarditis. | 2000 | 11120624 | |
| preventive effect of fk 506 (tacrolimus hydrate) on experimentally induced acute liver injury in rats. | the aim of the study was to investigate the effect of the immunosuppressant fk 506 (tacrolimus hydrate) on acute liver injury induced by propionibacterium acnes and lipopolysaccharide (lps). acute liver injury was induced in male wistar rats by injecting the animals with p. acnes (10 mg/rat), and administering lps (10 microg/rat) seven days later. one group was given fk 506 (1 mg/kg) 24 and 2 hr before administration of lps, and the other group was given the same dose of saline. the 24-hr surviv ... | 2000 | 11117573 |
| a high incidence of staphylococcus aureus colonization in the external eyes of patients with atopic dermatitis. | to determine the frequency distribution of bacteria on the external surface of eyes of patients with atopic dermatitis (ad) and to investigate the relationship between the frequency of bacterial colonization and the grade of atopy or ocular diseases associated with ad. | 2000 | 11097590 |
| the shampoo clue: two cases of infection of a ventriculoatrial shunt. | despite the use of sophisticated tools, infections of implanted devices may be difficult to diagnose. two cases of infections of ventriculoatrial shunts, which demonstrate the eminent importance of meticulously taking history, are reported and discussed. | 2000 | 11096029 |
| relationship between propionibacterium acnes biotypes and jumi-haidoku-to. | we examined the relationship between propionibacterium acnes biotypes and jumi-haidoku-to (jht). in all the p. acnes strains tested, the production of propionic acid (pa) and butyric acid (ba) was suppressed in a medium containing 1 mg/ml jht compared with the control medium without jht. there were no significant differences in the rates of decreased pa and ba production between p. acnes biotype 3 (b3) and the other biotypes or between isolates from mild skin rash and more severe skin rash. p. a ... | 2000 | 11092266 |
| biological properties associated with the enhanced lung-colonizing potential in a b16 murine melanoma line grown in a medium conditioned by syngeneic corynebacterium parvum-elicited macrophages. | a previous study by our laboratory showed that the peritoneal murine corynebacterium parnum-elicited macrophages released into their growth medium an activity which enhanced the ability of b16-f10 melanoma cells to form experimental metastases in the lung of syngeneic mice. in the present study, we used a clone of b16-f10 line (f10-m3 cells) to investigate whether the increase in lung-colonizing potential due to the pro-clonogenic activity released by c. parvum-elicited macrophages was associate ... | 1999 | 11089888 |
| ascorbate regeneration by the reduced form of 2-amino-3-carboxy-1, 4-naphthoquinone, a strong growth stimulator for bifidobacteria. | nonenzymatic reduction of dehydroascorbate into ascorbate by the reduced form (quinol form) of 2-amino-3-carboxy-1,4-naphthoquinone, a strong growth stimulator for bifidobacteria, has been found. the bimolecular reaction rate constant was evaluated as 9 m(-)(1) s(-)(1) at ph 7.0. this reaction has been successfully coupled with enzymatic regeneration of the naphthoquinol by nad(p)h in cell-free extracts of bifidobacterium longum 6001. the overall reaction is a regeneration of nad(p)(+) by dehydr ... | 2000 | 11087532 |
| the effects of amoxicillin therapy on skin flora in infants. | in order to determine the effect of amoxicillin therapy on the perineal skin microbial flora in infants, we took quantitative bacterial and fungal cultures of perineal and sternal areas from 25 infants treated with amoxicillin (40 mg/kg/day) for 10 days. specimens were obtained prior to therapy, within 3 days of conclusion of therapy, and 14-16 days later. immediately following therapy, a decline in the number of bacterial isolates occurred on both the perineum (89 to 47) and sternum (84 to 39). ... | 2000 | 11085662 |
| [microbiological flora cultured from peritoneal fluid of women in reproductive age]. | the aim of the study was to assess the presence of the microbes in peritoneal fluid of women in reproductive age. an investigation was performed on the group of 144 women operated laparoscopically in division of reproduction. the bacteria were cultured from 77 fluids (53.5%). the most common bacteria were: staphylococcus epidermidis (34.1%) and propionibacterium acnes (19.3%). in our group of patients 88 strains of bacteria were isolated. sensitivity to antibiotics differed in many points. this ... | 2000 | 11082969 |
| improvement of vitamin b(12) fermentation by reducing the inhibitory metabolites by cell recycle system and a mixed culture. | the major problem in vitamin b(12) production using propionibacterium is the growth inhibition of the cell due to the accumulation of inhibitory metabolites such as propionic acid and acetic acid. in the present paper, we considered several approaches of controlling the propionic acid concentration at low level. namely: (1) the periodic cultivation of propionibacterium where dissolved oxygen (do) concentration was alternatively changed between 0 and 1ppm; (2) cell recycle system using hollow fib ... | 2000 | 11080651 |
| physiological peculiarities of propionibacteria--present facts and prospective applications. | the genus propionibacterium includes dairy and cutaneous propionibacteria; they differ principally by their natural habitats: in milks, cheeses and on human skin and in the rumen ruminants respectively. using modern methods of molecular biology a new composition of the genus, containing at present 10 species was established. these bacteria generate energy by fermentation, which is linked with a short anaerobic respiration chain yielding more atp than in any other bacterial fermentation. propioni ... | 2000 | 11077481 |
| red light kills bacteria via photodynamic action. | with the increase in the number of antibiotic resistant strains of microorganism, the search for alternative treatments of microbial infections becomes all the more important. we report a novel method for bacterial inactivation based on the optical excitation of the naturally occurring (endogenous) photosensitzing porphyrins by red light. in particular, the pathogenic gram-positive porphyrin producing atcc strains propionibacterium acnes, actinomyces odontolyticus and porphyromonas gingivalis we ... | 2000 | 11075959 |
| in vitro and in vivo effects of an immunomodulator composed of escherichia coli lipopolysaccharide and propionibacterium granulosum-inactivated cells in pigs. | the in vitro cytokine profiles of porcine alveolar macrophages and peripheral blood mononuclear cells (pbmc) were examined by reverse transcription-polymerase chain reaction or enzyme-linked immunosorbent assay after stimulation with the immunomodulatory compound inmd [lipopolysaccharide (lps) and propionibacterium granulosum]. expression of interleukin-1 (il-1), il-6, il-12 and tumour necrosis factor-alpha (tnf-alpha), but not of il-10, was detected in inmd-stimulated alveolar macrophages. stim ... | 2000 | 11075550 |
| characterization of prgo1, a plasmid from propionibacterium acidipropionici, and its use for development of a host-vector system in propionibacteria. | the complete nucleotide sequence of prgo1, a cryptic plasmid from propionibacterium acidipropionici e214, was determined. prgo1 is 6, 868 bp long, and its g+c content is 65.0%. frame analysis of the sequence revealed six open reading frames, which were designated orf1 to orf6. the deduced amino acid sequences of orf1 and orf2 showed extensive similarities to an initiator of plasmid replication, the rep protein, of various plasmids of gram-positive bacteria. the amino acid sequence of the putativ ... | 2000 | 11055911 |
| proliferative response of peripheral blood mononuclear cells and levels of antibody to recombinant protein from propionibacterium acnes dna expression library in japanese patients with sarcoidosis. | the causes of sarcoidosis are unknown. propionibacterium acnes has been isolated from sarcoid lesions, and many genomes of p. acnes or p. granulosum have been detected in all biopsy samples tested from japanese patients with sarcoidosis. we searched for protein antigens from propionibacteria that caused immune responses in patients with sarcoidosis but not in subjects without sarcoidosis. | 2000 | 11033841 |
| the coenzyme b12 analog 5'-deoxyadenosylcobinamide-gdp supports catalysis by methylmalonyl-coa mutase in the absence of trans-ligand coordination. | methylmalonyl-coa mutase is an 5'-adenosylcobalamin (adocbl)-dependent enzyme that catalyzes the rearrangement of methylmalonyl-coa to succinyl-coa. the crystal structure of this protein revealed that binding of the cofactor is accompanied by a significant conformational change in which dimethylbenzimidazole, the lower axial ligand to cobalt in solution, is replaced by his(610) donated by the active site. the role of the lower axial ligand in the trillion-fold labilization of the upper axial cob ... | 2001 | 11031263 |
| heat shock proteins and inflammatory acne vulgaris: molecular cloning, overexpression and purification of a propionibacterium acnes groel and dnak homologue. | propionibacterium acnes is associated with inflammatory acne. the genes encoding two putative mediators of inflammation, the heat shock proteins groel and dnak, were cloned from this organism and sequenced. groel and dnak encode proteins of 56.8 and 66.4 kda, respectively, which show a high degree of homology (>75% similarity) to the groel and dnak proteins of mycobacteria and streptomycetes. the promoter regions of both genes contain inverted repeat sequences believed to be involved in the tran ... | 2000 | 11024261 |
| chemotactic cytokines (chemokines) in human hepatitis and experimental hepatitis models: which ones play the crucial role? | 2000 | 11023047 | |
| increased expression of osteopontin in activated kupffer cells and hepatic macrophages during macrophage migration in propionibacterium acnes-treated rat liver. | osteopontin is an extracellular matrix component that can act as a chemokine to induce macrophage migration. the significance of osteopontin in macrophage infiltration into the liver was examined in rats given heat-killed propionibacterium acnes. in normal rats, osteopontin mrna expression in the liver was minimal, determined by quantitative-competitive reverse transcription-polymerase chain reaction (rt-pcr) assay. northern blot analysis revealed that osteopontin mrna was not expressed in kupff ... | 2000 | 11023041 |
| effects of hepatocellular iron imbalance on nitric oxide and reactive oxygen intermediates production in a model of sepsis. | in mammals iron homeostasis is most important, as imbalance of iron such as iron overload may lead to severe diseases. recently, it has been shown that the iron regulatory protein-1 is partially controlled by nitric oxide and reactive oxygen intermediates, molecules frequently seen in inflammatory events. the aim of the present study was to investigate the effects of impaired iron homeostasis on the interaction of nitric oxide, and reactive oxygen intermediate formation in hepatocytes in a model ... | 2000 | 11019994 |
| biochemical and genetic characterization of propionicin t1, a new bacteriocin from propionibacterium thoenii. | a collection of propionibacteria was screened for bacteriocin production. a new bacteriocin named propionicin t1 was isolated from two strains of propionibacterium thoenii. this bacteriocin shows no sequence similarity to other bacteriocins. propionicin t1 was active against all strains of propionibacterium acidipropionici, propionibacterium thoenii, and propionibacterium jensenii tested and also against lactobacillus sake ncdo 2714 but showed no activity against propionibacterium freudenreichii ... | 2000 | 11010864 |
| [manganese-dependent ribonucleotide reductase from propionibacterium freudenreichii subsp. shermanii: partial purification, characteristics and role in dna biosynthesis]. | like lactobacillus leichmanii, rhizobium meliloti, and euglena gracilis, p. freudenreichii implicates cobalamin in dna anabolism via adenosylcobalamin-dependent ribonucleotide reductase. however, in the absence of corrinoids, p. freudenreichii is able to synthesize dna with the involvement of an alternative ribonucleotide reductase, which is independent of adenosylcobalamin. this enzyme is localized in both the cytoplasm (80% of activity) and the cytoplasmic membrane (20% of activity), being loo ... | 2000 | 11008681 |
| critical evaluation of the one- versus the two-channel model for the operation of the atp synthase's f(o) motor. | the mechanism of converting an electrochemical gradient of protons or na(+) ions across the membrane into rotational torque by the f(o) motor of the atp synthase has been described by a two-channel model or by a one-channel model. experimental evidence obtained with the f(o) motor from the propionigenium modestum atp synthase is described which is in accordance with the one-channel model, but not with the two-channel model. this evidence includes the atp-dependent occlusion of one (22)na(+) per ... | 2000 | 11004469 |
| comparative in vitro activities of gar-936 against aerobic and anaerobic animal and human bite wound pathogens. | gar-936 is a new semisynthetic glycylcycline with a broad antibacterial spectrum, including tetracycline-resistant strains. the in vitro activities of gar-936, minocycline, doxycycline, tetracycline, moxifloxacin, penicillin g, and erythromycin were determined by agar dilution methods against 268 aerobic and 148 anaerobic strains of bacteria (including pasteurella, eikenella, moraxella, bergeyella, neisseria, ef-4, bacteroides, prevotella, porphyromonas, fusobacterium, staphylococcus, streptococ ... | 2000 | 10991855 |
| correlation between propionibacterium acnes biotypes, lipase activity and rash degree in acne patients. | we examined the possible correlation between biotypes of propionibacterium acnes, lipase activity, and rash degree in acne patients. among 5 p. acnes biotypes, p. acnes biotype 3 (b3) was the most common, followed by p. acnes biotypes 1, 2 and 4; p. acnes biotype 5 was not found. p. acnes b3 was isolated from more severe skin rashes than those of the other biotypes. production of propionic acid (pa) and butyric acid (ba) by p. acnes b3 was higher than those by the other p. acnes biotypes. as the ... | 2000 | 10989576 |
| viability and beta-galactosidase activity of dairy propionibacteria subjected to digestion by artificial gastric and intestinal fluids. | an important criterion to consider in the selection of strains for dietary adjuncts is the ability of the microorganisms to survive the severe conditions of acidity and bile concentrations usually found in the gastrointestinal tract. in the present work, we report the effects of digestions by artificial gastric and intestinal fluids on beta-galactosidase activity and survival of four strains of dairy propionibacteria previously selected by their bile tolerance and beta-galactosidase activity. th ... | 2000 | 10983795 |
| in vitro activity of an evernimicin derivative, sch27899, against anaerobic bacteria and propionibacterium acnes. | the in vitro activity of sch27899, a novel oligosaccharide antimicrobial agent, was compared with those of representatives of six classes of antimicrobial agents (piperacillin, clarithromycin, clindamycin, vancomycin, sitafloxacin and metronidazole) against clinical isolates of anaerobic bacteria and propionibacterium acnes. against peptostreptococcus: spp. and clostridium difficile, sch27899 was the most potent (mic(90) < 0.125 mg/l) of the agents examined. besides these gram-positive anaerobes ... | 2000 | 10980176 |
| nitric oxide is neither necessary nor sufficient for resolution of plasmodium chabaudi malaria in mice. | malaria is a life-threatening re-emerging disease, yet it is still not clear how bloodstage malarial parasites are killed. nitric oxide (no), which has potent anti-microbial activity, may represent an important killing mechanism. the production of no during descending plasmodium chabaudi parasitemia, a period when parasites are killed by the immune response, supports this concept. however, nos20/0 and nos30/0 mice as well as mice treated with no synthase 2 (nos2) inhibitors do not develop exacer ... | 2000 | 10975849 |
| vitrectomy for endophthalmitis after intraocular lens implantation. | to report the results of vitrectomy and intraocular lens (iol) removal for the treatment of endophthalmitis after iol implantation. | 2000 | 10974303 |
| images in cardiology: mycotic aneurysm formation with dehiscence of a valved aortic conduit resulting in dynamic aortic obstruction. | 2000 | 10956288 | |
| management of chronic deep infection following rotator cuff repair. | deep infection of the shoulder following rotator cuff repair is uncommon. there are few reports in the literature regarding the management of such infections. | 2000 | 10954101 |
| [effective and safe pharmacotherapy of acne vulgaris and treatment of sun-damaged skin]. | an inevitable condition for the pharmacist is a basic knowledge of dermatological changes which are prominent in acne and solar impairment of the skin to be able to recommend in a qualified manner an effective and safe treatment to the patient. however, sufferers of the more serious forms of acne should always be referred to their general practitioner, or preferentially a dermatologist. acne vulgaris is an androgen-induced disorder, but three major mechanisms for the development of the disease h ... | 2000 | 10953445 |
| propionibacterium acnes endophthalmitis requiring intraocular lens removal after failure of medical therapy. | a 52-year-old saudi man developed intraocular inflammation 7 weeks after uneventful phacoemulsification with implantation of a silicone posterior chamber intraocular lens (iol). cultures from the aqueous and vitreous were repeatedly negative, but a temporary response to intracameral and intravitreal injection of vancomycin was noted. a series of 6 intraocular injections given over 5 days failed to resolve the inflammation. it did, however, disappear after the iol was removed. the iol provided th ... | 2000 | 10946205 |
| in vivo 13c nmr study of the bidirectional reactions of the wood-werkman cycle and around the pyruvate node in propionibacterium freudenreichii subsp. shermanii and propionibacterium acidipropionici. | this study used in vitro 13c nmr spectroscopy to directly examine bidirectional reactions of the wood-werkman cycle involved in central carbon metabolic pathways of dairy propionibacteria during pyruvate catabolism. the flow of [2-13c]pyruvate label was monitored on living cell suspensions of propionibacterium freudenreichii subsp. shermanii and propionibacterium acidipropionici under acidic conditions. p. shermanii and p. acidipropionici cells consumed pyruvate at apparent initial rates of 161 ... | 1999 | 10937824 |
| metabolic biotinylation of recombinant proteins in mammalian cells and in mice. | the avidin-biotin system is a fundamental technology in biomedicine for immunolocalization, imaging, nucleic acid blotting, and protein labeling. while this technology is robust, it is limited by the fact that mammalian proteins must be expressed and purified prior to chemical biotinylation using cross-linking agents which modify proteins at random locations to heterogeneous levels and can inactivate protein function. to circumvent this limitation, we demonstrate the ability to metabolically bio ... | 2000 | 10933917 |
| propionicin sm1, a bacteriocin from propionibacterium jensenii df1: isolation and characterization of the protein and its gene. | we purified a bacteriocin from the cell-free supernatant of propionibacterium jensenii df1 isolated from swiss raw milk, and named it propionicin sm1. the heat-stable protein was strongly bactericidal against p. jensenii dsm20274. on the basis of the n-terminal amino acid sequence of the purified protein, a degenerate oligonucleotide probe was designed to locate and clone the corresponding gene of p. jensenii df1. it hybridized exclusively with the df1l-resident plasmid plme106, but not with chr ... | 2000 | 10930068 |
| high-performance liquid chromatography analysis of porphyrins in propionibacterium acnes. | 2000 | 10929774 | |
| propionibacterium acnes as a cause of visually significant corneal ulcers. | to report propionibacterium acnes as a cause of vision-threatening infectious keratitis and to discuss culture isolation and antibiotic treatment. | 2000 | 10928755 |
| alcaligenes xylosoxidans and propionibacterium acnes postoperative endophthalmitis in a pseudophakic eye. | to report a case of persistent polymicrobial postoperative endophthalmitis caused by alcaligenes xylosoxidans and propionibacterium acnes in a pseudophakic eye. a. xylosoxidans is a gram-negative bacteria resistant to most antibiotics. | 2000 | 10927000 |
| protection of radical intermediates at the active site of adenosylcobalamin-dependent methylmalonyl-coa mutase. | adenosylcobalamin-dependent methylmalonyl-coa mutase catalyzes the interconversion of methylmalonyl-coa and succinyl-coa via radical intermediates generated by substrate-induced homolysis of the coenzyme carbon-cobalt bond. from the structure of methylmalonyl-coa mutase it is evident that the deeply buried active site is completely shielded from solvent with only a few polar contacts made between the protein and the substrate. site-directed mutants of amino acid his244, a residue close to the in ... | 2000 | 10924114 |
| [infectious osteoarthritis due to propionibacterium acnes. two new cases]. | propionibacterium acnes is an anaerobic germ, usually found as a saprophyte of the skin and the mucosa. it may be responsible for iatrogenic or spontaneous osteoarhritis. | 2000 | 10909155 |
| combination azelaic acid therapy for acne vulgaris. | there is no topical antiacne medication that acts against all four of the major pathophysiologic features of acne: hyperkeratinization, sebum production, bacterial proliferation, and inflammation. topical azelaic acid cream helps both to normalize keratinization and to reduce the proliferation of propionibacterium acnes, and has proven to be effective against both noninflammatory and inflammatory lesions. the results of a recent study now demonstrate that its efficacy can be enhanced, and patien ... | 2000 | 10898830 |
| adhesion of inactivated probiotic strains to intestinal mucus. | it has been suggested that probiotics should be viable in order to elicit beneficial health effects. inactivation of probiotics has been suggested to interfere with the binding to the mucosa and thereby with the immune modulating activity of probiotics. the effect of different inactivation methods on the mucus adhesion of nine probiotic strains was studied. inactivation by heat or gamma-irradiation generally decreased the adhesive abilities. however, heat treatment increased the adhesion of prop ... | 2000 | 10886621 |
| skin-specific caspase-1-transgenic mice show cutaneous apoptosis and pre-endotoxin shock condition with a high serum level of il-18. | to study the pathophysiological roles of overexpressed caspase-1 (casp1), originally designated as il-1 beta-converting enzyme, we generated transgenic mice in which human casp1 is overexpressed in their keratinocytes. the transgenic mice spontaneously developed recalcitrant dermatitis and skin ulcers, characterized by the presence of massive keratinocyte apoptosis. the skin of the mice contained the active form of human casp1 and expressed mrna for caspase-activated dnase, an effector endonucle ... | 2000 | 10878376 |
| the bacteriology of acne vulgaris and antimicrobial susceptibility of propionibacterium acnes and staphylococcus epidermidis isolated from acne lesions. | we examined the species of bacteria aerobically and anaerobically isolated from 30 acne lesions and determined antimicrobial susceptibilities of propionibacterium acnes (p. acnes) and staphylococcus epidermidis (s. epidermidis) using nine antimicrobial agents. among the bacteria isolated, s. epidermidis was most dominant. both p. acnes and s. epidermidis were isolated from half of the acne lesions. the mic of seven antimicrobials (ampicillin, erythromycin, roxithromycin, clindamycin, tetracyclin ... | 2000 | 10875198 |
| effects of znc-2381, a new oral compound, on several hepatic injury models and on hepatocellular apoptosis in mice and rats. | the hepatoprotective effect of znc-2381 (1-(4-aminophenyl) methyl-3-(3-nitrophenyl)-1,3-dihydroimidazo[4,5-b]pyridine-2-one), a novel 2-one dihydroimidazopyridine derivative, has been evaluated in several experimental models of hepatic injury. in mice, oral znc-2381, administered at doses of 3, 10 or 30 mgkg(-1), 1 h before induction of hepatic injury with concanavalin a, dose-dependently inhibited increases in serum alanine aminotransferase (alt) activity. apoptosis of liver cells, as indicated ... | 2000 | 10864141 |
| novel propionibacterium infection in cattle. | to describe four cases of infection in cattle, from geographically different places, with a presumptive new species of propionibacterium, which causes granulomatous lesions in the head, thorax, abdomen, pelvic area and skin. | 2000 | 10860156 |