Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| the multicopy srna lhrc controls expression of the oligopeptide-binding protein oppa in listeria monocytogenes. | listeria monocytogenes is the causative agent of the foodborne disease listeriosis. during infection, l. monocytogenes produces an array of non-coding rnas, including the multicopy srna lhrc. these five, nearly identical srnas are highly induced in response to cell envelope stress and target the virulence adhesin lapb at the post-transcriptional level. here, we demonstrate that lhrc controls expression of additional genes encoding cell envelope-associated proteins with virulence function. using ... | 2015 | 26176322 |
| a sensitive impedance biosensor based on immunomagnetic separation and urease catalysis for rapid detection of listeria monocytogenes using an immobilization-free interdigitated array microelectrode. | in this study, we described a novel impedance biosensor combining immunomagnetic separation with urease catalysis for sensitive detection of foodborne bacteria using listeria monocytogenes as model and an immobilization-free microelectrode as detector. the monoclonal antibodies (mabs) were immobilized on the surface of the magnetic nanoparticles (mnps) with the diameter of 180 nm by biotin-streptavidin system for specifically and efficiently separating listeria cells from sample background. the ... | 2015 | 26176211 |
| unusual presentation of listerial myocarditis and the diagnostic value of cardiac magnetic resonance. | listeria monocytogenes is an infrequent cause of bacterial myocarditis. myocarditis without evidence of endocarditis is even rarer. management in such cases involves early diagnosis, antibiotic therapy, and emergency treatment of arrhythmias. we report the case of a 47-year-old man who presented with features of acute st-segment-elevation myocardial infarction complicated by ventricular tachycardia that necessitated urgent electrical cardioversion. contrast-enhanced cardiac magnetic resonance im ... | 2015 | 26175642 |
| application of a novel antimicrobial coating on roast beef for inactivation and inhibition of listeria monocytogenes during storage. | the antilisterial efficacy of novel coating solutions made with organic acids, lauric arginate ester, and chitosan was evaluated in a three-stage study on inoculated roast beef for the first time. ready-to-eat roast beef was specially ordered from the manufacturer. the meat surface was inoculated with five-strain listeria monocytogenes cocktail inoculums at two different levels, ~3 and 6 log cfu/cm(2) and treated with the stock solution (hams), the 1:5 diluted solution (mams), and the 1:10 dilut ... | 2015 | 26173201 |
| lamellipodin is important for cell-to-cell spread and actin-based motility in listeria monocytogenes. | listeria monocytogenes is a foodborne pathogen capable of invading a broad range of cell types and replicating within the host cell cytoplasm. this paper describes the colocalization of host cell lamellipodin (lpd) with intracellular l. monocytogenes detectable 6 h postinfection of epithelial cells. the association was mediated via interactions between both the peckstrin homology (ph) domain in lpd and phosphatidylinositol (3,4)-bisphosphate [pi(3,4)p2] on the bacterial surface and by interactio ... | 2015 | 26169271 |
| alanine-scanning mutational analysis of durancin gl reveals residues important for its antimicrobial activity. | durancin gl is a novel class iia bacteriocin with 43 residues produced by enterococcus durans 41d. this bacteriocin demonstrates narrow inhibition spectrum and potent antimicrobial activity against several listeria monocytogenes strains, including nisin-resistant l. monocytogenes nr30. a systematic alanine-scanning mutational analysis with site-directed mutagenesis was performed to analyze durancin gl residues important for antimicrobial activity and specificity. results showed that three mutati ... | 2015 | 26168032 |
| fundamental characteristics of deep-uv light-emitting diodes and their application to control foodborne pathogens. | low-pressure mercury uv (lp-uv) lamps have long been used for bacterial inactivation, but due to certain disadvantages, such as the possibility of mercury leakage, deep-uv-c light-emitting diodes (duv-leds) for disinfection have recently been of great interest as an alternative. therefore, in this study, we examined the basic spectral properties of duv-leds and the effects of uv-c irradiation for inactivating foodborne pathogens, including escherichia coli o157:h7, salmonella enterica serovar ty ... | 2015 | 26162872 |
| pulsed-light inactivation of pathogenic and spoilage bacteria on cheese surface. | cheese products are susceptible to postprocessing cross-contamination by bacterial surface contamination during slicing, handling, or packaging, which can lead to food safety issues and significant losses due to spoilage. this study examined the effectiveness of pulsed-light (pl) treatment on the inactivation of the spoilage microorganism pseudomonas fluorescens, the nonenterohemorrhagic escherichia coli atcc 25922 (nonpathogenic surrogate of escherichia coli o157:h7), and listeria innocua (nonp ... | 2015 | 26162787 |
| stability, antimicrobial activity, and effect of nisin on the physico-chemical properties of fruit juices. | heat processing is the most commonly used hurdle for inactivating microorganisms in fruit juices. however, this preservation method could interfere with the organoleptic characteristics of the product. alternative methods have been proposed and bacteriocins such as nisin are potential candidates. however, the approval of bacteriocins as food additives is limited, especially in foods from vegetal origin. we aimed to verify the stability, the effect on physico-chemical properties, and the antimicr ... | 2015 | 26162590 |
| survival and metabolic activity of pediocin producer pediococcus acidilactici ul5: its impact on intestinal microbiota and listeria monocytogenes in a model of the human terminal ileum. | pediococcus acidilactici ul5 is a promising probiotic candidate due to its high survival rate under gastric and duodenal conditions and to its ability to produce the antilisterial bacteriocin pediocin pa-1. its survival, metabolic activity, and impact on listeria monocytogenes in a continuous stirred tank reactor containing immobilized human intestinal microbiota were studied over a period of 32 days of feeding a nutrient medium simulating ileal chyme. the impact of p. acidilactici ul5 on differ ... | 2016 | 26162534 |
| influence of general stress-response alternative sigma factors σ(s) (rpos) and σ(b) (sigb) on bacterial tolerance to the essential oils from origanum vulgare l. and rosmarinus officinalis l. and pulsed electric fields. | this study assessed the influence of general stress-response alternative sigma factors rpos (σ(s)) and sigb (σ(b)) on tolerance of escherichia coli (e. coli mg1655 and its isogenic mutant e. coli mg1655 δrpos) and listeria monocytogenes (l. monocytogenes egd-e and its isogenic mutant l. monocytogenes egd-e δsigb) to the essential oils (eos) from origanum vulgare l.-oregano (oveo) and rosmarinus officinalis l.-rosemary (roeo), as well as the changes in tolerance of parental and δrpos and δsigb mu ... | 2015 | 26159473 |
| detection of listeria monocytogenes in ready-to-eat foods sampled from a catering service in apulia, italy. | listeria monocytogenes is currently considered a relevant emerging food-borne pathogen. in particular, the european centre for disease prevention and control (ecdc) illustrates its widespread presence in different foods. in the present article, l. monocytogenes prevalence was estimated in cooked ready-to-eat foods sampled from a catering service in a apulia city, southern italy. | 2015 | 26152545 |
| spontaneous restoration of transplantation tolerance after acute rejection. | transplantation is a cure for end-stage organ failure but, in the absence of pharmacological immunosuppression, allogeneic organs are acutely rejected. such rejection invariably results in allosensitization and accelerated rejection of secondary donor-matched grafts. transplantation tolerance can be induced in animals and a subset of humans, and enables long-term acceptance of allografts without maintenance immunosuppression. however, graft rejection can occur long after a state of transplantati ... | 2015 | 26151823 |
| age-related gene expression differences in monocytes from human neonates, young adults, and older adults. | a variety of age-related differences in the innate and adaptive immune systems have been proposed to contribute to the increased susceptibility to infection of human neonates and older adults. the emergence of rna sequencing (rna-seq) provides an opportunity to obtain an unbiased, comprehensive, and quantitative view of gene expression differences in defined cell types from different age groups. an examination of ex vivo human monocyte responses to lipopolysaccharide stimulation or listeria mono ... | 2015 | 26147648 |
| cooperative transcriptional activation of antimicrobial genes by stat and nf-κb pathways by concerted recruitment of the mediator complex. | the transcriptional response to infection with the bacterium listeria monocytogenes (lm) requires cooperative signals of the type i interferon (ifn-i)-stimulated jak-stat and proinflammatory nf-κb pathways. using chip-seq analysis, we define genes induced in lm-infected macrophages through synergistic transcriptional activation by nf-κb and the ifn-i-activated transcription factor isgf3. using the nos2 and il6 genes as prime examples of this group, we show that nf-κb functions to recruit enzymes ... | 2015 | 26146080 |
| characterization of specific alleles in inla and prfa of listeria monocytogenes isolated from foods in osaka, japan and their ability to invade caco-2 cells. | listeria monocytogenes expresses the surface protein internalin a (inla), enabling the invasion of human intestinal epithelial cells to cause severe food-borne diseases. full-length sequence analysis of inla of 114 food isolates resulted in the detection of 29 isolates with a premature stop codon (pmsc) mutation and 6 isolates with 3-codon deletion mutations (aa 738 to 740) in inla. the isolates with inla pmscs demonstrated a significantly lower level of invasion than the other food isolates in ... | 2015 | 26143289 |
| in vitro probiotic characteristics of lactobacillus plantarum zdy 2013 and its modulatory effect on gut microbiota of mice. | lactobacillus plantarum zdy 2013, a novel strain isolated from chinese traditional fermented acid beans, was systematically evaluated for its survival capacity under stress conditions (ph, bile salt, simulated gastrointestinal tract, and antibiotics), production of exopolysaccharide and antagonism against 8 pathogens. its effect on mice gut microbiota was also investigated by quantitative pcr and pcr-denaturing gradient gel electrophoresis. the results showed that zdy 2013 can grow at ph 3.5 and ... | 2015 | 26142853 |
| selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (gtg)5-pcr fingerprinting. | the aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (lab) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. antimicrobial activity of lab was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of bacillus cereus, listeria monocytogenes, escherichia coli, staphy ... | 2014 | 26139877 |
| increased spread and replication efficiency of listeria monocytogenes in organotypic brain-slices is related to multilocus variable number of tandem repeat analysis (mlva) complex. | listeria (l.) monocytogenes causes fatal infections in many species including ruminants and humans. in ruminants, rhombencephalitis is the most prevalent form of listeriosis. using multilocus variable number tandem repeat analysis (mlva) we recently showed that l. monocytogenes isolates from ruminant rhombencephalitis cases are distributed over three genetic complexes (designated a, b and c). however, the majority of rhombencephalitis strains and virtually all those isolated from cattle cluster ... | 2015 | 26138984 |
| treatment with incomplete freund's adjuvant and listeria monocytogenes delays diabetes via an interleukin-17-secretion-independent pathway. | non-obese diabetes (nod) mice are widely used as an animal model in studies of type i diabetes (tid). treatment with complete freund's adjuvant (cfa) in pro-diabetic nod mice is known to inhibit disease progression by activating cd1d-specific natural killer (nk) t cells and inducing interleukin (il)-17 secretion in innate immune cells. the aim of the present study was to examine the effect of incomplete freund's adjuvant (ifa) and l. monocytogenes treatment on the development of tid in nod mice. ... | 2015 | 26136917 |
| cloning, expression analysis, and rna interference study of a horma domain containing autophagy-related gene 13 (atg13) from the coleopteran beetle, tenebrio molitor. | autophagy is a process that is necessary during starvation, as it replenishes metabolic precursors by eliminating damaged organelles. autophagy is mediated by more than 35 autophagy-related (atg) proteins that participate in the nucleation, elongation, and curving of the autophagosome membrane. in a pursuit to address the role of autophagy during development and immune resistance of the mealworm beetle, tenebrio molitor, we screened atg gene sequences from the whole-larva transcriptome database. ... | 2015 | 26136688 |
| myeloid sirtuin 2 expression does not impact long-term mycobacterium tuberculosis control. | sirtuins (sirts) regulate several cellular mechanisms through deacetylation of several transcription factors and enzymes. recently, sirt2 was shown to prevent the development of inflammatory processes and its expression favors acute listeria monocytogenes infection. the impact of this molecule in the context of chronic infections remains unknown. we found that specific sirt2 deletion in the myeloid lineage transiently increased mycobacterium tuberculosis load in the lungs and liver of conditiona ... | 2015 | 26135889 |
| defining and evaluating a core genome multilocus sequence typing scheme for whole-genome sequence-based typing of listeria monocytogenes. | whole-genome sequencing (wgs) has emerged today as an ultimate typing tool to characterize listeria monocytogenes outbreaks. however, data analysis and interlaboratory comparability of wgs data are still challenging for most public health laboratories. therefore, we have developed and evaluated a new l. monocytogenes typing scheme based on genome-wide gene-by-gene comparisons (core genome multilocus the sequence typing [cgmlst]) to allow for a unique typing nomenclature. initially, we determined ... | 2015 | 26135865 |
| using the chicken embryo to assess virulence of listeria monocytogenes and to model other microbial infections. | microbial infections are a global health problem, particularly as microbes are continually developing resistance to antimicrobial treatments. an effective and reliable method for testing the virulence of different microbial pathogens is therefore a useful research tool. this protocol describes how the chicken embryo can be used as a trustworthy, inexpensive, ethically desirable and quickly accessible model to assess the virulence of the human bacterial pathogen listeria monocytogenes, which can ... | 2015 | 26134955 |
| listeria meningitis complicating alemtuzumab treatment in multiple sclerosis--report of two cases. | alemtuzumab, a humanized monoclonal antibody targeting the surface molecule cd52, leads to a rapid depletion of immune cells in the innate and adaptive immune system. in phase 2 and 3 trials in multiple sclerosis (ms), infections have been reported more frequently in alemtuzumab than in interferon beta treated patients. here we report two patients treated with alemtuzumab for ms developing listeria meningitis few days after the first infusion cycle. both patients recovered completely after promp ... | 2015 | 26132570 |
| evaluation of synergistic antibacterial and antioxidant efficacy of essential oils of spices and herbs in combination. | the present study was carried out to evaluate the possible synergistic interactions on antibacterial and antioxidant efficacy of essential oils of some selected spices and herbs [bay leaf, black pepper, coriander (seed and leaf), cumin, garlic, ginger, mustard, onion and turmeric] in combination. antibacterial combination effect was evaluated against six important food-borne bacteria (bacillus cereus, listeria monocytogenes, micrococcus luteus, staphylococcus aureus, escherichia coli and salmone ... | 2015 | 26132146 |
| solitary supratentorial listeria monocytogenes brain abscess in an immunocompromised patient. | we describe an 81-year-old man receiving azacitidine monotherapy for myelodysplastic syndrome who was improving from listeria monocytogenes bacteremia after receiving antibiotic therapy during an earlier hospital admission. shortly after discharge he developed new-onset seizure activity, with brain imaging on subsequent admissions demonstrating a posterior right frontal lobe mass. specimen cultures after resection of the mass revealed this to be a cerebral abscess related to l. monocytogenes. br ... | 2015 | 26130881 |
| different types of stainless steel used in equipment in meat plants do not affect the initial microbial transfer, including pathogens, from pork skin. | this study describes and measures the impact of different compositions and finishes of stainless steel used in equipment in the meat industry on the transfer of natural flora and selected pathogens from artificially contaminated pork skin. it is known that the adhesion to surfaces of listeria monocytogenes and salmonella, 2 pathogens frequently found in contaminated pork meat, depends on the nature and roughness of the surface. our results show no statistically significant differences in microbi ... | 2015 | 26130860 |
| diadenylate cyclase evaluation of ssdaca (ssu98_1483) in streptococcus suis serotype 2. | cyclic diadenosine monophosphate is a recently identified signaling molecule. it has been shown to play important roles in bacterial pathogenesis. ssu98_1483 (ssdaca), which is an ortholog of listeria monocytogenes daca, is a putative diadenylate cyclase in streptococcus suis serotype 2. in this study, we determined the enzymatic activity of ssdaca in vitro using high-performance liquid chromatography and mass spectrometry. our results showed that ssdaca was a diadenylate cyclase that converts a ... | 2015 | 26125899 |
| ultraviolet-c light inactivation of escherichia coli o157:h7 and listeria monocytogenes on organic fruit surfaces. | this study investigated uv-c light inactivation of escherichia coli o157:h7 and listeria monocytogenes on the surface of organic apples, pears, strawberries, red raspberries and cantaloupes. fruit surfaces spot inoculated with cocktail strains of e. coli o157:h7 and l. monocytogenes were exposed to uv-c doses up to 11.9 kj/m(2) at 23 °c. fruit surface roughness, contact angle, and surface energy were determined and correlated with uv-c inactivation kinetics. results demonstrate that bacterial pa ... | 2015 | 26122954 |
| rescue of cd8+ t cell vaccine memory following sublethal γ irradiation. | sublethal γ irradiation eliminates cd8+ t cell mediated memory responses. in this work, we explored how these memory responses could be rescued in the aftermath of such exposure. we utilized two models of cd8+ t cell mediated immunity: a mouse model of listeria monocytogenes (lm) infection in which cd8+ t cells specific for lm expressed antigens (listeriolysin o, llo) can be tracked, and a murine skin graft model in which cd8+ t cells mediate rejection across a mhc class i (d(d)) disparity. in t ... | 2015 | 26122582 |
| multistate outbreak of listeriosis caused by imported cheese and evidence of cross-contamination of other cheeses, usa, 2012. | listeria monocytogenes is a foodborne pathogen that can cause bacteraemia, meningitis, and complications during pregnancy. in july 2012, molecular subtyping identified indistinguishable l. monocytogenes isolates from six patients and two samples of different cut and repackaged cheeses. a multistate outbreak investigation was initiated. initial analyses identified an association between eating soft cheese and outbreak-related illness (odds ratio 17·3, 95% confidence interval 2·0-825·7) but no com ... | 2016 | 26122394 |
| lactobacillus kunkeei yb38 from honeybee products enhances iga production in healthy adults. | to identify lactic acid bacterial isolates, which promote immunoglobulin a (iga) production in honeybee products and honeybees (apis mellifera). | 2015 | 26121394 |
| whole-genome sequencing allows for improved identification of persistent listeria monocytogenes in food-associated environments. | while the food-borne pathogen listeria monocytogenes can persist in food associated environments, there are no whole-genome sequence (wgs) based methods to differentiate persistent from sporadic strains. whole-genome sequencing of 188 isolates from a longitudinal study of l. monocytogenes in retail delis was used to (i) apply single-nucleotide polymorphism (snp)-based phylogenetics for subtyping of l. monocytogenes, (ii) use snp counts to differentiate persistent from repeatedly reintroduced str ... | 2015 | 26116683 |
| spatial and temporal factors associated with an increased prevalence of listeria monocytogenes in spinach fields in new york state. | while rain and irrigation events have been associated with an increased prevalence of foodborne pathogens in produce production environments, quantitative data are needed to determine the effects of various spatial and temporal factors on the risk of produce contamination following these events. this study was performed to quantify these effects and to determine the impact of rain and irrigation events on the detection frequency and diversity of listeria species (including l. monocytogenes) and ... | 2015 | 26116668 |
| mtor complex signaling through the sema4a-plexin b2 axis is required for optimal activation and differentiation of cd8+ t cells. | mammalian target of rapamycin (mtor) plays crucial roles in activation and differentiation of diverse types of immune cells. although several lines of evidence have demonstrated the importance of mtor-mediated signals in cd4(+) t cell responses, the involvement of mtor in cd8(+) t cell responses is not fully understood. in this study, we show that a class iv semaphorin, sema4a, regulates cd8(+) t cell activation and differentiation through activation of mtor complex (mtorc) 1. sema4a(-/-) cd8(+) ... | 2015 | 26116513 |
| dissecting cd8+ nkt cell responses to listeria infection reveals a component of innate resistance. | a small pool of nk1.1(+) cd8(+) t cells is harbored among the conventional cd8(+) t cell compartment. conclusions drawn from the analysis of immune responses mediated by cytotoxic cd8(+) t cells are often based on the total population, which includes these contaminating nk1.1(+) cd8(+) t cells. an unresolved question is whether nk1.1(+) cd8(+) cells are conventional t cells that acquire nk1.1 expression upon activation or delineation into memory phenotype or whether they are a distinct cell popu ... | 2015 | 26116500 |
| microbiological survey of raw and ready-to-eat leafy green vegetables marketed in italy. | the presence of foodborne pathogens (salmonella spp., listeria monocytogenes, escherichia coli o157:h7, thermotolerant campylobacter, yersinia enterocolitica and norovirus) in fresh leafy (fl) and ready-to-eat (rte) vegetable products, sampled at random on the italian market, was investigated to evaluate the level of risk to consumers. nine regional laboratories, representing 18 of the 20 regions of italy and in which 97.7% of the country's population resides, were involved in this study. all la ... | 2015 | 26114592 |
| sepsis caused by listeria monocytogenes during chemotherapy for small cell carcinoma of the thymus. | listeria monocytogenes is a facultative intracellular parasitic bacterium that is gram positive, catalase positive, oxidase negative, and a facultative anaerobe. it is known to infect humans through food. it is a bacillus with low virulence, but can cause meningitis and sepsis in infants and immunocompromised patients. | 2015 | 26111524 |
| a microplate growth inhibition assay for screening bacteriocins against listeria monocytogenes to differentiate their mode-of-action. | lactic acid bacteria (lab) have historically been used in food fermentations to preserve foods and are generally-recognized-as-safe (gras) by the fda for use as food ingredients. in addition to lactic acid; some strains also produce bacteriocins that have been proposed for use as food preservatives. in this study we examined the inhibition of listeria monocytogenes 39-2 by neutralized and non-neutralized bacteriocin preparations (bac+ preps) produced by lactobacillus curvatus fs47; lb. curvatus ... | 2015 | 26111195 |
| antimicrobial mechanism of resveratrol-trans-dihydrodimer produced from peroxidase-catalyzed oxidation of resveratrol. | plant polyphenols are known to have varying antimicrobial potencies, including direct antibacterial activity, synergism with antibiotics and suppression of bacterial virulence. we performed the in vitro oligomerization of resveratrol catalyzed by soybean peroxidase, and the two isomers (resveratrol-trans-dihydrodimer and pallidol) produced were tested for antimicrobial activity. the resveratrol-trans-dihydrodimer displayed antimicrobial activity against the gram-positive bacteria bacillus cereus ... | 2015 | 26109045 |
| [regularities of the ubiquitous polyhostal microorganisms selection by the example of three taxa]. | the investigation of the bacterial populations' heterogeneity contributes to the control of natural foci, causative agents of nosocomial infections, to the analysis of the microbial evolution. multilocus sequence typing (mlst) was employed for the analysis of the diversity and features of the distribution of polyhostal ubiquitous microorganisms of the genera burkholderia, leptospira, and listeria, which belong to three bacterial phyla: proteobacteria, spirochaetes, and firmicutes. according to t ... | 2015 | 26107896 |
| potential of bacteriocin-producing lactic acid bacteria for safety improvements of traditional thai fermented meat and human health. | lactic acid bacteria (lab) are very important in converting of agricultural products into safe, delicious and shelf stable foods for human consumption. the preservative activity of lab in foods is mainly attributed to the production of anti-microbial metabolites such as organic acids and bacteriocins which enables them to grow and control the growth of pathogens and spoilage microorganisms. besides ensuring safety, bacteriocin-producing lab with their probiotic potentials could also be emerging ... | 2015 | 26100576 |
| an outbreak of an unusual strain of listeria monocytogenes infection in north-east scotland. | listeria monocytogenes infection is an important cause of illness and hospitalization in vulnerable individuals. in the present study, we describe a community outbreak of listeria monocytogenes in the north-east region of scotland, which was epidemiologically, environmentally and microbiologically linked to a local meat product and ready-to-eat product manufacturer. infected individuals were interviewed, and an environmental investigation was conducted. clinical and environmental samples were te ... | 2015 | 26100074 |
| inactivation of pathogenic bacteria inoculated onto a bacto™ agar model surface using tio2-uvc photocatalysis, uvc and chlorine treatments. | the aim of this study was to study inactivation of different pathogenic bacteria on agar model surface using tio2-uv photocatalysis (tuvp). | 2015 | 26099167 |
| identification of high-risk listeria monocytogenes serotypes in lineage i (serotype 1/2a, 1/2c, 3a and 3c) using multiplex pcr. | using molecular subtyping techniques, listeria monocytogenes is divided into three major phylogenetic lineages, and a multiplex pcr method can differentiate five l. monocytogenes subgroups: 1/2a-3a, 1/2c-3c, 1/2b-3b-7, 4b-4d-4e and 4a-4c. in this study, we conducted genome comparisons and evaluated serotype-associated genes for their utility as a multiplex pcr-based method for distinguishing high-risk serotypes 1/2a and 1/2c in lineage i from low-risk serotypes 3a and 3c. | 2015 | 26095922 |
| characterization of lactobacillus fermentum pl9988 isolated from healthy elderly korean in a longevity village. | in this work, we wanted to develop a probiotic from famous longevity villages in korea. we visited eight longevity villages in korea to collect fecal samples from healthy adults who were aged above 80 years and had regular bowel movements, and isolated lactic-acid-producing bacteria from the samples. isolated colonies that appeared on mrs agar containing bromophenol blue were identified by means of 16s rrna sequencing, and 102 of the isolates were identified as lactic-acid-producing bacteria (18 ... | 2015 | 26095384 |
| cold plasma inactivation of internalised bacteria and biofilms for salmonella enterica serovar typhimurium, listeria monocytogenes and escherichia coli. | microbial biofilms and bacteria internalised in produce tissue may reduce the effectiveness of decontamination methods. in this study, the inactivation efficacy of in-package atmospheric cold plasma (acp) afterglow was investigated against salmonella typhimurium, listeria monocytogenes and escherichia coli in the forms of planktonic cultures, biofilms formed on lettuce and associated bacteria internalised in lettuce tissue. prepared lettuce broth (3%) was inoculated with bacteria resulting in a ... | 2015 | 26093991 |
| spreading of multiple listeria monocytogenes abscesses via central nervous system fiber tracts: case report. | animal studies have shown that listeria monocytogenes can probably access the brain through a peripheral intraneural route, and it has been suggested that a similar process may occur in humans. however, thus far, its spreading through the central nervous system (cns) has not been completely elucidated. the authors present a case of multiple l. monocytogenes cerebral abscesses characterized by a pattern of distribution that suggested spread along white matter fiber tracts and reviewed the literat ... | 2015 | 26090836 |
| listeriolysin o affects the permeability of caco-2 monolayer in a pore-dependent and ca2+-independent manner. | listeria monocytogenes is a food and soil-borne pathogen that secretes a pore-forming toxin listeriolysin o (llo) as its major virulence factor. we tested the effects of llo on an intestinal epithelial cell line caco-2 and compared them to an unrelated pore-forming toxin equinatoxin ii (eqtii). results showed that apical application of both toxins causes a significant drop in transepithelial electrical resistance (teer), with higher llo concentrations or prolonged exposure time needed to achieve ... | 2015 | 26087154 |
| antimicrobial activity of thyme oil co-nanoemulsified with sodium caseinate and lecithin. | emulsions of essential oils are investigated as potential intervention strategies to improve food safety and are preferably prepared from generally-recognized-as-safe emulsifiers. stable thyme oil nanoemulsions can be prepared using combinations of sodium caseinate (nacas) and soy lecithin. the objective of the present research was to study the antimicrobial activity of these nanoemulsions and understand the impacts of emulsifier concentrations. 10 g/l thyme oil was emulsified using combinations ... | 2015 | 26082324 |
| amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films. | silver nanoparticles (agnps) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films. the agnps solutions exhibited characteristic absorption peak at 420 nm that showed a red shift to ∼434 nm after forming composite with agar. xrd data demonstrated the crystalline structure of agnps with dominant (111) facet. apparent surface color and transmittance of agar films were greatly in ... | 2015 | 26076636 |
| bacteriophage module reshuffling results in adaptive host range as exemplified by the baseplate model of listerial phage a118. | each phage infects its specific bacterial host strain through highly specific interactions between the baseplate-associated receptor binding protein (rbp) at the tip of the phage tail and the receptor at the host surface. baseplates incorporate structural core modules, dit and tal, largely conserved among phages, and peripheral modules anchoring the rbps. exploiting structural information from the hhpred program and em data from the bielmann et al. (2015) paper, a molecular model of the a118 pha ... | 2015 | 26074066 |
| listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review. | listeriosis is an infectious and fatal disease of animals, birds, fish, crustaceans and humans. it is an important food-borne zoonosis caused by listeria monocytogenes, an intracellular pathogen with unique potential to spread from cell to cell, thereby crossing blood-brain, intestinal and placental barriers. the organism possesses a pile of virulence factors that help to infect the host and evade from host immune machinery. though disease occurrence is sporadic throughout the world, it can resu ... | 2015 | 26073265 |
| development of a new paenibacillin-producing strain and testing its usability in improving food safety. | a new bacterial strain that produces a bacteriocin (paenibacillin) without polymyxin was developed from paenibacillus polymyxa that co-produces the 2 antimicrobial agents. gamma radiation was used successfully to develop the new strain, p. polymyxa osy-hg. subsequently, we explored the feasibility of using food or food ingredients as growth media for the new strain. milk supported the growth of p. polymyxa osy-hg which produced up to 32 mg paenibacillin/l milk without polymyxin. fermentation cru ... | 2015 | 26073160 |
| protozoan cysts act as a survival niche and protective shelter for foodborne pathogenic bacteria. | the production of cysts, an integral part of the life cycle of many free-living protozoa, allows these organisms to survive adverse environmental conditions. given the prevalence of free-living protozoa in food-related environments, it is hypothesized that these organisms play an important yet currently underinvestigated role in the epidemiology of foodborne pathogenic bacteria. intracystic bacterial survival is highly relevant, as this would allow bacteria to survive the stringent cleaning and ... | 2015 | 26070667 |
| genome sequence of listeria monocytogenes strain hpb5415, collected during a 2008 listeriosis outbreak in canada. | listeria monocytogenes strain hpb5415-isolated from deli meat-was found in 2008 to have the same pulsed-field gel electrophoresis patterns as a clinical strain (08-5923). however, whether nucleotide differences (single nucleotide polymorphisms [snps]) exist between their genomes was not determined. we sequenced the l. monocytogenes strain hpb5415 genome and identified 52 snps relative to strain 08-5923. | 2015 | 26067972 |
| complete genome sequence of listeria monocytogenes strain dpc6895, a serotype 1/2b isolate from bovine raw milk. | listeria monocytogenes is a foodborne pathogen and is the causative agent of listeriosis among humans and animals. the draft genome sequence of l. monocytogenes dpc6895, a serotype 1/2b strain isolated from the raw milk of a cow with subclinical bovine mastitis, is reported. | 2015 | 26067969 |
| expression of virulence-related genes in listeria monocytogenes grown on danish hard cheese as affected by nacl content. | expression of virulence-related genes in listeria monocytogenes incubated on cheese was assessed by real-time quantitative polymerase chain reaction. the objective of the study was to investigate the impact of sodium chloride concentration in cheese on transcription of virulence genes and, thereby, virulence potential of l. monocytogenes. the expression studies were performed with l. monocytogenes strains characterized by different tolerance to salt stress. strains atcc(®) 51779 and dsmz 15675 w ... | 2015 | 26067229 |
| bacterial enteric infections among older adults in the united states: foodborne diseases active surveillance network, 1996-2012. | a growing segment of the population-adults aged ≥65 years-is more susceptible than younger adults to certain enteric (including foodborne) infections and experience more severe disease. | 2015 | 26067228 |
| fermentation of table olives by oleuropeinolytic starter culture in reduced salt brines and inactivation of escherichia coli o157:h7 and listeria monocytogenes. | the effect of an autochthonous starter culture developed by oleuropeinolytic strains belonging to the lactobacillus plantarum group on the physicochemical and microbiological characteristics and the biophenol content of table olives fermented under reduced salt conditions was studied. black (cv. kalamata) and green (cv. chalkidikis) olives were fermented in two different kinds of brine (brine a containing 2.3% nacl, 32.3mm ca-acetate and 33.9mm ca-lactate and brine b containing 4% nacl, ph5.0 in ... | 2015 | 26065729 |
| assessing bacterial invasion of cardiac cells in culture and heart colonization in infected mice using listeria monocytogenes. | listeria monocytogenes is a gram-positive facultative intracellular pathogen that is capable of causing serious invasive infections in immunocompromised patients, the elderly, and pregnant women. the most common manifestations of listeriosis in humans include meningitis, encephalitis, and fetal abortion. a significant but much less documented sequelae of invasive l. monocytogenes infection involves the heart. the death rate from cardiac illness can be up to 35% despite treatment, however very li ... | 2015 | 26065439 |
| experimental application of lactobacillus fermentum ccm 7421 in combination with chlorophyllin in dogs. | chlorophyll belongs in a larger class of phytochemical plant pigments currently receiving more attention as a physiologically active dietary component. although most research has focused on its biological activities such as its antioxidant, antimutagenic, anti-inflammatory or apoptotic effects in humans or rodents, there is limited knowledge at this time about the combinative possibilities of chlorophyll with probiotic bacteria. our aim was to test the growth characteristics of canine-derived pr ... | 2015 | 26062536 |
| bacterial contamination of iranian paper currency. | transmission of human pathogens can be occurred via inert objects. paper currency is a further common contact surface whereby pathogens can be transferred within a population although the significance remains unknown. hence, the aim of the present study was to investigate microbial populations associated with iranian paper currency. | 2013 | 26060669 |
| activation of prfa results in overexpression of virulence factors but does not rescue the pathogenicity of listeria monocytogenes m7. | listeria monocytogenes encodes a transcriptional activator, prfa, to positively regulate the expression of virulence factors. several mutations in prfa (prfa*) have been found to contribute to increased regulatory activity. here, we describe a strain, m7, containing a prfa*(g145s) that activates expression of virulence factors but with low pathogenicity. to study this contradictory relationship, we exchanged the prfa genes between strains egde and m7 (designated egde-prfa(m7) and m7-prfa(egde)). ... | 2015 | 26055558 |
| inactivation of escherichia coli o157:h7 and listeria monocytogenes in biofilms by pulsed ultraviolet light. | the inactivation of biofilms formed by pathogenic bacteria on ready-to-eat and minimally processed fruits and vegetables by nonthermal processing methods is critical to ensure food safety. pulsed ultraviolet (puv) light has shown promise in the surface decontamination of liquid, powdered, and solid foods. in this study, the antimicrobial efficacy of puv light treatment on nascent biofilms formed by escherichia coli o157:h7 and listeria monocytogenes on the surfaces of food packaging materials, s ... | 2015 | 26054759 |
| atp binding drives substrate capture in an ecf transporter by a release-and-catch mechanism. | ecf transporters are a family of active transporters for vitamins. they are composed of four subunits: a membrane-embedded substrate-binding subunit (ecfs), a transmembrane coupling subunit (ecft) and two atp-binding-cassette atpases (ecfa and ecfa'). we have investigated the mechanism of the ecf transporter for riboflavin from the pathogen listeria monocytogenes, lmecf-ribu. using structural and biochemical approaches, we found that atp binding to the ecfaa' atpases drives a conformational chan ... | 2015 | 26052893 |
| the rab5-gef function of rin1 regulates multiple steps during listeria monocytogenes infection. | 2015 | 26050999 | |
| synergistic effect of nisin and garlic shoot juice against listeria monocytogenes in milk. | the aim of this research was to determine the synergistic effect of nisin and garlic shoot juice (gsj) against listeria monocytogenes atcc 19118 found in whole (3.5%), low (1%) and skim (no fat content) milk. garlic shoot juice (gsj) at concentrations of 2.5%, 5% and 10% revealed strong and similar patterns of antilisterial effect against l. monocytogenes atcc 19118 in all categories of milk. nisin only at concentrations of 62.5, 125, 250 and 500iu/ml displayed a strong antilisterial effect as c ... | 2008 | 26049229 |
| quantifying viable vibrio parahaemolyticus and listeria monocytogenes simultaneously in raw shrimp. | a novel taqman-based multiplex real-time pcr method combined with propidium monoazide (pma) treatment was firstly developed for the simultaneous quantification of viable vibrio parahaemolyticus and listeria monocytogenes in raw shrimp. the optimization of pma concentration showed that 100 μm was considered optimal to effectively inhibit 10(8) cfu/ml dead cells of both bacteria. the high specificity of this method was confirmed on tests using 96 target and non-target strains. the optimized assay ... | 2015 | 26048473 |
| inactivation of listeria monocytogenes using water bath heat treatment in vacuum packed ricotta salata cheese wedges. | ricotta salata cheese is frequently contaminated on the surface with listeria monocytogenes. water bath heat treatment in vacuum packed whole ricotta salata cheese wheels demonstrated to be effective in inactivating l. monocytogenes. however, the risk of cross-contamination in ricotta salata wedges is increased during cheese cutting. therefore, the effectiveness of heat treatment in ricotta salata wedges has to be demonstrated conducting a new validation study. in this study, 9 different time te ... | 2015 | 26044297 |
| impact of cell-free supernatant of lactic acid bacteria on putrescine and other polyamine formation by foodborne pathogens in ornithine decarboxylase broth. | conversion of ornithine to putrescine by salmonella paratyphi a, listeria monocytogenes, staphylococcus aureus, and escherichia coli was investigated in ornithine decarboxylase broth (odb) using cell-free supernatants (cfss) obtained from leuconostoc mesenterodies subsp. cremoris, pediococcus acidilactici, lactococcus lactis subsp. lactis, streptococcus thermophilus. two groups of cell-free supernatants (25 or 50%) and control (only odb) were prepared to investigate putrescine (put) and other po ... | 2015 | 26043863 |
| effect of milk fat content on the performance of ohmic heating for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium and listeria monocytogenes. | the effect of milk fat content on ohmic heating compared to conventional heating for inactivation of food-borne pathogens was investigated. | 2015 | 26043029 |
| application of bacteriophages in post-harvest control of human pathogenic and food spoiling bacteria. | bacteriophages have attracted great attention for application in food biopreservation. lytic bacteriophages specific for human pathogenic bacteria can be isolated from natural sources such as animal feces or industrial wastes where the target bacteria inhabit. lytic bacteriophages have been tested in different food systems for inactivation of main food-borne pathogens including listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, salmonella enterica, shigella spp., campylobac ... | 2016 | 26042353 |
| structural analysis and molecular docking of potential ligands with chorismate synthase of listeria monocytogenes: a novel antibacterial drug target. | listeriosis, in particular that caused by listeria monocytogenes, is a major foodborne pathogen, and its control is becoming difficult because of widespread emergence of drug resistance strains. chorismate synthase (cs), an essential enzyme of shikimate pathway present only in bacteria, fungi, plant and some apicomplexan parasites, is a validated potential antimicrobial drug target. antimicrobial development through the elucidation of essential structural features of the cs of l. monocytogenes ( ... | 2015 | 26040111 |
| comparative effects of ohmic and conventional heating for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in skim milk and cream. | ohmic heating has proven advantages over conventional thermal processing and novel thermal alternative technologies. in this study, the effect of ohmic and conventional heating for pasteurizing skim milk and cream was examined. all treatment conditions for ohmic and conventional heating were identical except for composition of the heating chamber. in most cases, the reduction of three pathogens did not differ significantly between ohmic heating and conventional heating at fixed treatment tempera ... | 2015 | 26038915 |
| effect of acidified sorbate solutions on the lag-phase durations and growth rates of listeria monocytogenes on meat surfaces. | the surfaces of ready-to-eat meats are susceptible to postprocessing contamination by listeria monocytogenes. this study quantified the lag-phase durations (lpd) and growth rates (gr) of l. monocytogenes on the surfaces of cooked ham as affected by sorbate solutions of different concentrations and ph levels. slices of cooked ham inoculated with a four-strain mixture of l. monocytogenes (ca. 10(3) cfu/g) were surface treated with sorbate solutions of 0 to 4% (wt/vol) at ph 4.0 to 6.5, vacuum pack ... | 2015 | 26038906 |
| comparative study on the efficacy of bacteriophages, sanitizers, and uv light treatments to control listeria monocytogenes on sliced mushrooms (agaricus bisporus). | the following reports on a comparative study on the efficacy of different decontamination technologies to decrease listeria monocytogenes inoculated onto white sliced mushrooms and assesses the fate of residual levels during posttreatment storage under aerobic conditions at 8 °c. the treatments were chemical (hydrogen peroxide, peroxyacetic acid, ozonated water, electrolyzed water, chitosan, lactic acid), biological (listeria bacteriophages), and physical (uv-c, uv-hydrogen peroxide). none of th ... | 2015 | 26038905 |
| irrigation is significantly associated with an increased prevalence of listeria monocytogenes in produce production environments in new york state. | environmental (i.e., meteorological and landscape) factors and management practices can affect the prevalence of foodborne pathogens in produce production environments. this study was conducted to determine the prevalence of listeria monocytogenes, listeria species (including l. monocytogenes), salmonella, and shiga toxin-producing escherichia coli (stec) in produce production environments and to identify environmental factors and management practices associated with their isolation. ten produce ... | 2015 | 26038903 |
| growth of salmonella enterica and listeria monocytogenes on fresh-cut cantaloupe under different temperature abuse scenarios. | effective cold chain management is a critical component of food safety practice. in this study, we examined the impact of commonly encountered temperature abuse scenarios on the proliferation of salmonella enterica and listeria monocytogenes on fresh-cut cantaloupe. inoculated fresh-cut cantaloupe cubes were subjected to various temperature abuse conditions, and the growth of s. enterica and l. monocytogenes was determined. during 1 week of storage, salmonella cell counts on fresh-cut cantaloupe ... | 2015 | 26038902 |
| a novel suicide plasmid for efficient gene mutation in listeria monocytogenes. | although several plasmids have been used in listeria monocytogenes for generating mutants by allelic exchange, construction of l. monocytogenes mutants has been inefficient due to lack of effective selection markers for first and second recombination events. to address this problem, we have developed a new suicide plasmid, phoss1, by using the pmad plasmid backbone and anhydrotetracycline selection marker (secy antisense rna) driven by an inducible pxyl/teto promoter. expression of the secy anti ... | 2015 | 26038185 |
| kinetics of bacterial inactivation by 405nm and 520nm light emitting diodes and the role of endogenous coproporphyrin on bacterial susceptibility. | photodynamic inactivation studies of microbial pathogens have focused on the use of an external photosensitizer or a precursor compound to eliminate bacteria. the present study investigated the inactivation kinetics of six bacterial pathogens by a 405nm light emitting diode (led) without the addition of any external compound. the role of endogenous coproporphyrin on the bacterial susceptibility to leds was also examined. pathogens were illuminated with leds at 25, 10 and 4°c for 9h and the inact ... | 2015 | 26036659 |
| a case report of listeria monocytogenes abscesses presenting as cortically predominant ring-enhancing lesions. | listeria monocytogenes, a common cause of bacterial meningitis, rarely involves the central nervous system (cns) in the form of multiple cerebral ring-enhancing lesions. | 2017 | 26034484 |
| microinjection of francisella tularensis and listeria monocytogenes reveals the importance of bacterial and host factors for successful replication. | certain intracellular bacteria use the host cell cytosol as the replicative niche. although it has been hypothesized that the successful exploitation of this compartment requires a unique metabolic adaptation, supportive evidence is lacking. for francisella tularensis, many genes of the francisella pathogenicity island (fpi) are essential for intracellular growth, and therefore, fpi mutants are useful tools for understanding the prerequisites of intracytosolic replication. we compared the growth ... | 2015 | 26034213 |
| epidemiology of bacteremia in previously healthy febrile infants: a follow-up study. | describe the etiology of bacteremia among a geographically diverse sample of previously well infants with fever admitted for general pediatric care and to characterize demographic and clinical characteristics of infants with bacteremia according to bacterial etiology. we hypothesized that the epidemiology of bacteremia in febrile infants from a geographically diverse cohort would show similar results to smaller or single-center cohorts previously reported. | 2015 | 26034160 |
| the elimination of listeria monocytogenes attached to stainless steel or aluminum using multiple hurdles. | ready-to-eat luncheon meats sliced in retail delis have been found to pose the greatest risk of foodborne illness from listeria monocytogenes among all ready-to-eat foods. slicers used in delis have many removable parts that are connected with seals and gaskets, with spaces, cracks and crevices that are difficult to clean adequately and may provide a niche for l. monocytogenes survival. standard cleaning and sanitizing practices used by deli employees may not eliminate listeria in these niches. ... | 2015 | 26033521 |
| libyan thymus capitatus essential oil: antioxidant, antimicrobial, cytotoxic and colon pathogen adhesion-inhibition properties. | in the present work, the libyan wild-growing thymus capitatus essential oil (eo) was evaluated for its biological properties. | 2015 | 26033505 |
| inactivation of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in apple juice at different ph levels by gaseous ozone treatment. | we investigated the effect of ozone treatment of apple juice at different ph levels for inactivation of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes. | 2015 | 26033289 |
| synergistic effect of slightly acidic electrolyzed water and ultrasound at mild heat temperature in microbial reduction and shelf-life extension of fresh-cut bell pepper. | the objectives of this study were to evaluate the effect of combined treatments (slightly acidic electrolyzed water (saew), ultrasound (us), or mild heat (60°c)) on the growth of listeria monocytogenes and salmonella enterica serovar typhimurium in fresh-cut bell pepper, and the shelf-life and sensory quality (color and texture) were followed during storage at 4°c and 25°c. an additional 0.65, 1.72, and 2.70 log cfu/g reduction was achieved by heat treatments at 60°c for 1 min for dw, saew, and ... | 2015 | 26032362 |
| the sactibiotic subclass of bacteriocins: an update. | the sactibiotics are a recently designated subclass of bacteriocins that contain characteristic cysteine sulphur to α -carbon linkages mediated through post-translational modifications. they are a relatively small subclass of bacteriocins compared to the most thoroughly studied lantibiotics. the sactibiotics that have been extensively studied thus far are thuricin cd, subtilosin a, thurincin h, and propionicin f. despite their recent discovery, there have already been significant advances made i ... | 2015 | 26031307 |
| detection of poultry meat specific bacteria using ftir spectroscopy and chemometrics. | ftir spectra of poultry meat specific bacteria viz. salmonella enteritidis, pseudomonas ludensis, listeria monocytogenes and escherichia coli were collected and investigated for identification of spectral windows capable of bacterial classification and quantification. two separate datasets obtained at different times were used in the study to check reproducibility of results. multivariate data analysis techniques viz. principal component analysis (pca), partial least-squares discriminant analysi ... | 2015 | 26028771 |
| the pamp c-di-amp is essential for listeria monocytogenes growth in rich but not minimal media due to a toxic increase in (p)ppgpp. [corrected]. | cyclic di-adenosine monophosphate (c-di-amp) is a widely distributed second messenger that appears to be essential in multiple bacterial species, including the gram-positive facultative intracellular pathogen listeria monocytogenes. in this study, the only l. monocytogenes diadenylate cyclase gene, daca, was deleted using a cre-lox system activated during infection of cultured macrophages. all δdaca strains recovered from infected cells harbored one or more suppressor mutations that allowed grow ... | 2015 | 26028365 |
| phenotypic heterogeneity, a phenomenon that may explain why quorum sensing does not always result in truly homogenous cell behavior. | phenotypic heterogeneity describes the occurrence of "nonconformist" cells within an isogenic population. the nonconformists show an expression profile partially different from that of the remainder of the population. phenotypic heterogeneity affects many aspects of the different bacterial lifestyles, and it is assumed that it increases bacterial fitness and the chances for survival of the whole population or smaller subpopulations in unfavorable environments. well-known examples for phenotypic ... | 2015 | 26025903 |
| genes associated with desiccation and osmotic stress in listeria monocytogenes as revealed by insertional mutagenesis. | listeria monocytogenes is a foodborne pathogen whose survival in food processing environments may be associated with its tolerance to desiccation. to probe the molecular mechanisms used by this bacterium to adapt to desiccation stress, a transposon library of 11,700 l. monocytogenes mutants was screened, using a microplate assay, for strains displaying increased or decreased desiccation survival (43% relative humidity, 15°c) in tryptic soy broth (tsb). the desiccation phenotypes of selected muta ... | 2015 | 26025900 |
| two variants of selenium-dependent glutathione peroxidase from the disk abalone haliotis discus discus: molecular characterization and immune responses to bacterial and viral stresses. | glutathione peroxidase (gpx) is an essential member of the antioxidant systems of living organisms and may be involved in immune defense against pathogenic invasion. in the current study, two selenium-dependent glutathione peroxidases (absegpxs) that shared 54.3% identity were identified from the disk abalone haliotis discus discus. the open reading frames (orfs) of absegpx-a and absegpx-b coded for 222 and 220 amino acids, respectively, with a characteristic selenocysteine residue encoded by an ... | 2015 | 26025184 |
| complete genome sequence of listeria monocytogenes n2306, a strain associated with the 2013-2014 listeriosis outbreak in switzerland. | we present the complete genome sequence of listeria monocytogenes n2306, a serotype 4b clinical strain isolated during the 2013-2014 nationwide listeriosis outbreak in switzerland. | 2015 | 26021930 |
| bacterial spread from cell to cell: beyond actin-based motility. | several intracellular pathogens display the ability to propagate within host tissues by displaying actin-based motility in the cytosol of infected cells. as motile bacteria reach cell-cell contacts they form plasma membrane protrusions that project into adjacent cells and resolve into vacuoles from which the pathogen escapes, thereby achieving spread from cell to cell. seminal studies have defined the bacterial and cellular factors that support actin-based motility. by contrast, the mechanisms s ... | 2015 | 26021574 |
| a comparison of in-house real-time lamp assays with a commercial assay for the detection of pathogenic bacteria. | molecular detection of bacterial pathogens based on lamp methods is a faster and simpler approach than conventional culture methods. although different lamp-based methods for pathogenic bacterial detection are available, a systematic comparison of these different lamp assays has not been performed. in this paper, we compared 12 in-house real-time lamp assays with a commercialized kit (isothermal master mix) for the detection of listeria monocytogenes, salmonella spp, staphylococcus aureus, esche ... | 2015 | 26020698 |
| proteolytic and antimicrobial activity of lactic acid bacteria grown in goat milk. | we examined 62 strains and 21 trade starter cultures from the collection of lb bulgaricum plc for proteolytic and antimicrobial activity of lactic acid bacteria (lab) grown in goat milk. the aim of this study was to investigate the fermentation of caseins, α-lactalbumin and β-lactoglobulin by lab, using the o-phthaldialdehyde (opa) spectrophotometric assay and sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page). the proteolysis targeted mainly caseins, especially β-casein. whey ... | 2014 | 26019593 |
| genotyping of listeria monocytogenes isolates from poultry carcasses using high resolution melting (hrm) analysis. | an outbreak situation of human listeriosis requires a fast and accurate protocol for typing listeria monocytogenes. existing techniques are either characterized by low discriminatory power or are laborious and require several days to give a final result. polymerase chain reaction (pcr) coupled with high resolution melting (hrm) analysis was investigated in this study as an alternative tool for a rapid and precise genotyping of l. monocytogenes isolates. fifty-five isolates of l. monocytogenes is ... | 2014 | 26019495 |