Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [lyme disease in pediatrics: different faces, the same disease. cases series]. | lyme disease is caused by borrelia burgdorferi infection which is transmitted by ixodes ricinus. erythema migrans, a rash spreading from the site of a tick bite, is the earliest and most common manifestation of the disease. if untreated, late manifestations of disseminated disease, mainly neurological and musculoskeletal, may occur. serologic studies are usually negative in early stages and are not necessary to confirm the diagnosis in cases of erythema migrans. laboratory confirmation is needed ... | 2015 | 25996338 |
| evolution of the translocation and assembly module (tam). | bacterial outer membrane proteins require the beta-barrel assembly machinery (bam) for their correct folding and function. the central component of this machinery is bama, an omp85 protein that is essential and found in all gram-negative bacteria. an additional feature of the bam is the translocation and assembly module (tam), comprised tama (an omp85 family protein) and tamb. we report that tama and a closely related protein taml are confined almost exclusively to proteobacteria and bacteroidet ... | 2015 | 25994932 |
| study of a cohort of 1,886 persons to determine changes in antibody reactivity to borrelia burgdorferi 3 months after a tick bite. | lyme borreliosis is a tick-borne disease caused by the bacterium borrelia burgdorferi. the most frequent clinical manifestation is a rash called erythema migrans. changes in antibody reactivity to b. burgdorferi 3 months after a tick bite are measured using enzyme-linked immunosorbent assays (elisas). one assay is based on native purified flagellum antigen (igg), and the other assay is based on a recombinant antigen called c6 (igg or igm). paired samples were taken at the time of a tick bite and ... | 2015 | 25994550 |
| interactions between tick and transmitted pathogens evolved to minimise competition through nested and coherent networks. | natural foci of ticks, pathogens, and vertebrate reservoirs display complex relationships that are key to the circulation of pathogens and infection dynamics through the landscape. however, knowledge of the interaction networks involved in transmission of tick-borne pathogens are limited because empirical studies are commonly incomplete or performed at small spatial scales. here, we applied the methodology of ecological networks to quantify >14,000 interactions among ticks, vertebrates, and path ... | 2015 | 25993662 |
| oligoarthritis caused by borrelia bavariensis, austria, 2014. | a case of lyme oligoarthritis occurred in an 11-year-old boy in vienna, austria. dna of borrelia bavariensis was detected by pcr in 2 aspirates obtained from different joints. complete recovery was achieved after a 4-week course with amoxicillin. lyme arthritis must be considered in patients from europe who have persisting joint effusions. | 2015 | 25992945 |
| lyme meningo-radiculitis responsive to oral doxycycline therapy in the usa. | the spirochete strains that cause lyme disease are different between the usa and europe. this leads not only to a variation in clinical presentations, but it was also thought to alter responsiveness to antibiotic treatment. unlike in europe, in the usa there are no head-to-head trials of oral and intravenous antibiotics in the treatment of neuroborreliosis. guidelines from the american academy of neurology (aan) state that oral doxycycline is probably safe and effective in treating neuroborrlios ... | 2014 | 25988066 |
| tick bite with target lesion complicating routine cast immobilization. | 2015 | 25987946 | |
| cyclic di-gmp modulates gene expression in lyme disease spirochetes at the tick-mammal interface to promote spirochete survival during the blood meal and tick-to-mammal transmission. | borrelia burgdorferi, the lyme disease spirochete, couples environmental sensing and gene regulation primarily via the hk1/rrp1 two-component system (tcs) and rrp2/rpon/rpos pathways. beginning with acquisition, we reevaluated the contribution of these pathways to spirochete survival and gene regulation throughout the enzootic cycle. live imaging of b. burgdorferi caught in the act of being acquired revealed that the absence of rpos and the consequent derepression of tick-phase genes impart a st ... | 2015 | 25987708 |
| tick-borne bacteria in ixodes ricinus collected in southern norway evaluated by a commercial kit and established real-time pcr protocols. | ticks are important vectors of human pathogens. the knowledge of disease causing agents harboured by ticks in norway is limited. the focus of this study was (a) to detect the bacteria of medical importance in ticks collected from the vegetation at locations in the southern part of the country and (b) to evaluate a novel commercially available multiplex pcr based method by comparing results with conventional established real-time pcr protocols. borrelia burgdorferi sensu lato was confirmed to be ... | 2015 | 25985721 |
| tick-borne encephalitis: a review of epidemiology, clinical characteristics, and management. | tick-borne encephalitis is an infection of central nervous system caused by tick-borne encephalitis virus transmitted to humans predominantly by tick bites. during the last few decades the incidence of the disease has been increasing and poses a growing health problem in almost all endemic european and asian countries. most cases occur during the highest period of tick activity, in central europe mainly from april to november. tick-borne encephalitis is more common in adults than in children. cl ... | 2015 | 25984517 |
| iga nephropathy in a patient presenting with scleritis. | scleritis is a very uncommon manifestation in patients with iga nephropathy. here, we report the case of a patient presenting with diffuse anterior scleritis in which the laboratory disclosed microscopic haematuria and nephrotic range proteinuria. renal function was normal. serology for lupus, vasculitis and cryoglobulinaemia was negative. rheumatoid factor was negative, and serum c3 and serum c4 were on the normal range. serology for human immunodeficiency virus types 1 and 2, hepatitis b, hepa ... | 2010 | 25984051 |
| membranous glomerulonephritis secondary to borrelia burgdorferi infection presenting as nephrotic syndrome. | 2009 | 25984044 | |
| membranous glomerulonephritis secondary to borrelia burgdorferi infection presenting as nephrotic syndrome. | 2009 | 25984044 | |
| peripheral blood eosinophilia and hypersensitivity reactions among patients receiving outpatient parenteral antibiotics. | although drug-induced peripheral eosinophilia complicates antimicrobial therapy, little is known about its frequency and implications. | 2015 | 25981739 |
| ticks and tick-borne pathogens in south bohemia (czech republic)--spatial variability in ixodes ricinus abundance, borrelia burgdorferi and tick-borne encephalitis virus prevalence. | spatial distribution of ixodes ricinus tick host-seeking activity, as well as prevalence of borrelia burgdorferi sensu lato and tick-borne encephalitis virus (tbev) were studied in the tbe endemic area of south bohemia (czech republic). high variability in tick abundance detected in a network of 30 study sites was most closely associated with characteristics of vegetation cover. of 11,182 tested tick samples, 12% carried dna of spirochete from b. burgdorferi s.l. complex. b. afzelii and b. garin ... | 2015 | 25976235 |
| invariant natural killer t cells and their ligands: focus on multiple sclerosis. | invariant natural killer t (inkt) cells are an innate population of t cells identified by the expression of an invariant t-cell receptor and reactivity to lipid-based antigens complexed with cd1d. they account for a small percentage of lymphocytes, but are extremely potent and play central roles in immunity to infection, in some cancers, and in autoimmunity. the list of relevant stimulatory lipids and glycolipid antigens now includes a range of endogenous self-antigens including the myelin-deriv ... | 2015 | 25976210 |
| the chemokine cxcl13 is elevated in the cerebrospinal fluid of patients with neurosyphilis. | the chemokine cxcl13 has been discussed as a diagnostic parameter with high specificity for lyme neuroborreliosis (lnb) and as a marker of disease activity. neurosyphilis and lnb share similar characteristics. we investigated retrospectively cxcl13 levels in the cerebrospinal fluid (csf) of patients with neurosyphilis at initial diagnosis and during treatment. | 2015 | 25975424 |
| tick sialostatins l and l2 differentially influence dendritic cell responses to borrelia spirochetes. | transmission of pathogens by ticks is greatly supported by tick saliva released during feeding. dendritic cells (dc) act as immunological sentinels and interconnect the innate and adaptive immune system. they control polarization of the immune response towards th1 or th2 phenotype. we investigated whether salivary cystatins from the hard tick ixodes scapularis, sialostatin l (sialo l) and sialostatin l2 (sialo l2), influence mouse dendritic cells exposed to borrelia burgdorferi and relevant toll ... | 2015 | 25975355 |
| cross-reactive epitopes in borrelia burgdorferi p66. | epitope mapping of the p66 outer membrane protein of borrelia burgdorferi revealed that the protein contains numerous cross-reactive linear epitopes recognized by serum antibody in the majority of individuals tested, regardless of lyme disease history, limiting the usefulness of this antigen in lyme disease serodiagnostic assays. | 2015 | 25972406 |
| vaccination with the ospa- and ospb-negative borrelia burgdorferi strain 50772 provides significant protection against canine lyme disease. | beagles received placebo or ospa- and ospb-negative borrelia burgdorferi before a tick challenge. a total of 28 (41%) ticks and skin biopsy specimens from each control dog (n = 10) contained b. burgdorferi. in contrast, 12 (19%) ticks recovered from the vaccine recipients (n = 10) were infected (p = 0.0077), and 5 dogs yielded spirochetes from the skin biopsy specimens (p = 0.0325). in addition, 9 (90%) placebo recipients and 4 (40%) vaccine recipients developed joint abnormalities (p = 0.0573). ... | 2015 | 25972405 |
| deep sequencing analysis of the ixodes ricinus haemocytome. | ixodes ricinus is the main tick vector of the microbes that cause lyme disease and tick-borne encephalitis in europe. pathogens transmitted by ticks have to overcome innate immunity barriers present in tick tissues, including midgut, salivary glands epithelia and the hemocoel. molecularly, invertebrate immunity is initiated when pathogen recognition molecules trigger serum or cellular signalling cascades leading to the production of antimicrobials, pathogen opsonization and phagocytosis. we pres ... | 2015 | 25970599 |
| association of lyme disease and schizoaffective disorder, bipolar type: is it inflammation mediated? | lyme disease has been reported to be associated with various psychiatric presentations. borreliaburgdorferi (bb) can present with symptoms similar to schizophrenia and bipolar disorder. it has been suggested that inflammation incurred during the bb infection leads to neurodegenerative changes that result in schizophrenia-like presentations. we report a case of a 41-year-old male with a past history of bb infection who presents with psychosis. later in the course of his hospitalization, he develo ... | 2017 | 25969618 |
| passive surveillance for ticks on horses in saskatchewan. | passive surveillance of ticks on horses in saskatchewan revealed that the horses were parasitized by 3 species, dermacentor albipictus, d. andersoni, and d. variabilis. the nymphs and adults of d. albipictus occurred on horses earlier in the year than did adults of the 2 other species. | 2015 | 25969582 |
| mutations in the borrelia burgdorferi flagellar type iii secretion system genes flih and flii profoundly affect spirochete flagellar assembly, morphology, motility, structure, and cell division. | the lyme disease spirochete borrelia burgdorferi migrates to distant sites in the tick vectors and mammalian hosts through robust motility and chemotaxis activities. flih and flii are two cytoplasmic proteins that play important roles in the type iii secretion system (t3ss)-mediated export and assembly of flagellar structural proteins. however, detailed analyses of the roles of flih and flii in b. burgdorferi have not been reported. in this study, flih and flii transposon mutants were utilized t ... | 2015 | 25968649 |
| combination of loop-mediated isothermal amplification assay and nested pcr for detection of borrelia burgdorferi sensu lato in human serum samples. | a set of universal loop-mediated isothermal amplification (lamp) primers targeting the fla gene was designed to detect borrelia burgdorferi sensu lato (b. burgdorferi s.l.) in human samples. the sensitivity of lamp was 20 copies/reaction, and the assay did not detect false positives among 11 other related bacteria. a positive lamp result was obtained for 9 of the 24 confirmed cases and for 12 of 94 suspected cases. the positive rate of lamp was the same as that of nested pcr. the lamp is a useful ... | 2015 | 25966759 |
| the human antimicrobial protein calgranulin c participates in control of helicobacter pylori growth and regulation of virulence. | during infectious processes, antimicrobial proteins are produced by both epithelial cells and innate immune cells. some of these antimicrobial molecules function by targeting transition metals and sequestering these metals in a process referred to as "nutritional immunity." this chelation strategy ultimately starves invading pathogens, limiting their growth within the vertebrate host. recent evidence suggests that these metal-binding antimicrobial molecules have the capacity to affect bacterial ... | 2015 | 25964473 |
| neglected bacterial zoonoses. | bacterial zoonoses comprise a group of diseases in humans or animals acquired by direct contact with or by oral consumption of contaminated animal materials, or via arthropod vectors. among neglected infections, bacterial zoonoses are among the most neglected given emerging data on incidence and prevalence as causes of acute febrile illness, even in areas where recognized neglected tropical diseases occur frequently. although many other bacterial infections could also be considered in this negle ... | 2015 | 25964152 |
| a case of radiation-induced generalized morphea with prominent mucin deposition and tenderness. | radiation-induced morphea is a rare complication of radiation therapy. the affected areas are generally restricted to the radiation field or to the nearby surrounding area. | 2015 | 25958415 |
| conserved amblyomma americanum tick serpin19, an inhibitor of blood clotting factors xa and xia, trypsin and plasmin, has anti-haemostatic functions. | tick saliva serine protease inhibitors (serpins) facilitate tick blood meal feeding through inhibition of protease mediators of host defense pathways. we previously identified a highly conserved amblyomma americanum serpin 19 that is characterised by its reactive center loop being 100% conserved in ixodid ticks. in this study, biochemical characterisation reveals that the ubiquitously transcribed a. americanum serpin 19 is an anti-coagulant protein, inhibiting the activity of five of the eight s ... | 2015 | 25957161 |
| locating relevant patient information in electronic health record data using representations of clinical concepts and database structures. | clinicians and clinical researchers often seek information in electronic health records (ehrs) that are relevant to some concept of interest, such as a disease or finding. the heterogeneous nature of ehrs can complicate retrieval, risking incomplete results. we frame this problem as the presence of two gaps: 1) a gap between clinical concepts and their representations in ehr data and 2) a gap between data representations and their locations within ehr data structures. we bridge these gaps with a ... | 2014 | 25954405 |
| babesia spp. and other pathogens in ticks recovered from domestic dogs in denmark. | newly recognized endemic foci for human babesiosis include europe, where ixodes ricinus, a vector for several species of babesia, is the most commonly identified tick. vector-based surveillance provides an early warning system for the emergence of human babesiosis, which is likely to be under-reported at emerging sites. in the present study, we set out to screen i. ricinus collected from danish domestic dogs for babesia, in order to identify whether humans in denmark are exposed to the parasite. | 2015 | 25951937 |
| effect of liposomal clodronate-dependent depletion of professional antigen presenting cells on numbers and phenotype of canine cd4+cd25+foxp3+ regulatory t cells. | regulatory t cells (tregs) are known to control autoreactivity during and subsequent to the development of the peripheral immune system. professional antigen presenting cells (apcs), dendritic cells (dcs) and monocytes, have an important role in inducing tregs. for the first time, this study evaluated proportions and phenotypes of tregs in canine peripheral blood depleted of professional apcs, utilizing liposomal clodronate (lc) and multicolor flow cytometry analysis. our results demonstrate tha ... | 2014 | 25950023 |
| non-randall proliferative glomerulonephritis with humps and monotypic igg deposits in primary sjögren's syndrome: a first case report. | renal involvement is frequent in patients suffering from primary sjögren's syndrome (pss). tubulointerstitial infiltration is the most common renal lesion, while glomerular involvement is rare. we report the case of a 50-year-old woman with pss who developed renal failure due to an unusual proliferative glomerulonephritis with humps and monotypic igg1-kappa deposits. searches for cryoglobulinaemia, anti-double-stranded dna and anti-neutrophil cytoplasmic antibodies were negative. serum protein e ... | 2010 | 25949467 |
| infection of interleukin 17 receptor a-deficient c3h mice with borrelia burgdorferi does not affect their development of lyme arthritis and carditis. | recently, a number of studies have reported the presence of interleukin 17 (il-17) in patients with lyme disease, and several murine studies have suggested a role for this cytokine in the development of lyme arthritis. however, the role of il-17 has not been studied using the experimental lyme borreliosis model of infection of c3h mice with borrelia burgdorferi. in the current study, we investigated the role of il-17 in the development of experimental lyme borreliosis by infecting c3h mice devoi ... | 2015 | 25939508 |
| mutational analysis of the chlamydia muridarum plasticity zone. | pathogenically diverse chlamydia spp. can have surprisingly similar genomes. chlamydia trachomatis isolates that cause trachoma, sexually transmitted genital tract infections (chlamydia), and invasive lymphogranuloma venereum (lgv) and the murine strain chlamydia muridarum share 99% of their gene content. a region of high genomic diversity between chlamydia spp. termed the plasticity zone (pz) may encode niche-specific virulence determinants that dictate pathogenic diversity. we hypothesized tha ... | 2015 | 25939505 |
| recent-onset dilated cardiomyopathy associated with borrelia burgdorferi infection. | several recent small studies have suggested a causal link between lyme disease and dilated cardiomyopathy (dcm) by demonstrating the presence of the borrelia burgdorferi (bb) genome in the myocardium of patients with recent-onset dcm. the aim of this study was to further investigate the effect of targeted antibiotic treatment of bb-related recent-onset dcm in a larger cohort of patients. | 2015 | 25939436 |
| tick microbiome: the force within. | ticks are obligate blood-feeders and serve as vectors of human and livestock pathogens worldwide. defining the tick microbiome and deciphering the interactions between the tick and its symbiotic bacteria in the context of tick development and pathogen transmission will likely reveal new insights and spawn new paradigms to control tick-borne diseases. descriptive observations on the tick microbiome that began almost a century ago serve as forerunners to the gathering momentum to define the tick m ... | 2015 | 25936226 |
| target activation by regulatory rnas in bacteria. | bacterial small regulatory rnas (srnas) are commonly known to repress gene expression by base pairing to target mrnas. in many cases, srnas base pair with and sequester mrna ribosome-binding sites, resulting in translational repression and accelerated transcript decay. in contrast, a growing number of examples of translational activation and mrna stabilization by srnas have now been documented. a given srna often employs a conserved region to interact with and regulate both repressed and activat ... | 2015 | 25934124 |
| mechanism of immunosuppressants combined with cord blood for severe aplastic anemia. | this study aims to explore the mechanism of immunosuppressants combined with cord blood (is + cbi) for severe aplastic anemia. selecting 30 patients with saa and all treated with is + cbi (newly diagnosed group). 23 patients who were treated effectively (effective group) while 7 cases were treated invalidly (invalid group). another 20 healthy individuals were selected as control group. to detect the expression levels of il-17, il-22 and other cytokines by elisa method in each group. to detect th ... | 2015 | 25932194 |
| historic evidence to support a causal relationship between spirochetal infections and alzheimer's disease. | following previous observations a statistically significant association between various types of spirochetes and alzheimer's disease (ad) fulfilled hill's criteria in favor of a causal relationship. if spirochetal infections can indeed cause ad, the pathological and biological hallmarks of ad should also occur in syphilitic dementia. to answer this question, observations and illustrations on the detection of spirochetes in the atrophic form of general paresis, which is known to be associated wit ... | 2015 | 25932012 |
| lyme disease bacterium does not affect attraction to rodent odour in the tick vector. | vector-borne pathogens experience a conflict of interest when the arthropod vector chooses a vertebrate host that is incompetent for pathogen transmission. the qualitative manipulation hypothesis suggests that vector-borne pathogens can resolve this conflict in their favour by manipulating the host choice behaviour of the arthropod vector. | 2015 | 25928557 |
| mechanisms of antimicrobial peptide resistance in gram-negative bacteria. | cationic antimicrobial peptides (camps) are important innate immune defenses that inhibit colonization by pathogens and contribute to clearance of infections. gram-negative bacterial pathogens are a major target, yet many of them have evolved mechanisms to resist these antimicrobials. these resistance mechanisms can be critical contributors to bacterial virulence and are often crucial for survival within the host. here, we summarize methods used by gram-negative bacteria to resist camps. underst ... | 2014 | 25927010 |
| mechanisms of antimicrobial peptide resistance in gram-negative bacteria. | cationic antimicrobial peptides (camps) are important innate immune defenses that inhibit colonization by pathogens and contribute to clearance of infections. gram-negative bacterial pathogens are a major target, yet many of them have evolved mechanisms to resist these antimicrobials. these resistance mechanisms can be critical contributors to bacterial virulence and are often crucial for survival within the host. here, we summarize methods used by gram-negative bacteria to resist camps. underst ... | 2014 | 25927010 |
| stable expression of lentiviral antigens by quality-controlled recombinant mycobacterium bovis bcg vectors. | the well-established safety profile of the tuberculosis vaccine strain, mycobacterium bovis bacille calmette-guérin (bcg), makes it an attractive vehicle for heterologous expression of antigens from clinically relevant pathogens. however, successful generation of recombinant bcg strains possessing consistent insert expression has encountered challenges in stability. here, we describe a method for the development of large recombinant bcg accession lots which stably express the lentiviral antigens ... | 2015 | 25924766 |
| [lyme carditis]. | untreated lyme disease can affect the heart in up to 10% of patients. its clinical outcome and severity vary, ranging from asymptomatic minor conduction disturbances to potentially fatal arrhythmias and severe heart failure. a history of a tick bite or a typical previous skin lesion (erythema migrans) may be absent; clinicians should therefore keep a low threshold of suspicion when facing cardiac manifestations in a patient potentially exposed to the disease in endemic areas. we report the case ... | 2015 | 25924252 |
| new-onset panic, depression with suicidal thoughts, and somatic symptoms in a patient with a history of lyme disease. | lyme disease, or lyme borreliosis, caused by borrelia burgdorferi and spread by ticks, is mainly known to cause arthritis and neurological disorders but can also cause psychiatric symptoms such as depression and anxiety. we present a case of a 37-year-old man with no known psychiatric history who developed panic attacks, severe depressive symptoms and suicidal ideation, and neuromuscular complaints including back spasms, joint pain, myalgias, and neuropathic pain. these symptoms began 2 years af ... | 2015 | 25922779 |
| molecular pathogenesis of malt lymphoma. | approximately 8% of all non-hodgkin lymphomas are extranodal marginal zone b cell lymphoma of mucosa associated lymphoid tissue (malt), also known as malt lymphoma, which was first described in 1983 by isaacson and wright. malt lymphomas arise at a wide range of different extranodal sites, with the highest frequency in the stomach, followed by lung, ocular adnexa, and thyroid, and with a low percentage in the small intestine. interestingly, at least 3 different, apparently site-specific, chromos ... | 2015 | 25922601 |
| on-off kinetics of engagement of fni modules of soluble fibronectin by β-strand addition. | intrinsically disordered sequences within bacterial adhesins bind to e-strands in the β-sheets of multiple fni modules of fibronectin (fn) by anti-parallel β-strand addition, also called tandem β-zipper formation. the fud segment of sfbi of streptococcus pyogenes and bbk32 segment of bbk32 of borrelia burgdorferi, despite being imbedded in different adhesins from different bacteria, target the same 2-5,8-9 fni modules, 2-5,8-9 fni, in the n-terminal 70-kda region (fn70k) of fn. to facilitate fur ... | 2015 | 25919138 |
| concurrent gastric and pulmonary mucosa-associated lymphoid tissue lymphomas with pre-existing intrinsic chronic inflammation: a case report and a review of the literature. | 2015 | 25918263 | |
| escherichia coli yqja, a member of the conserved deda/tvp38 membrane protein family, is a putative osmosensing transporter required for growth at alkaline ph. | the ability to persist and grow under alkaline conditions is an important characteristic of many bacteria. in order to survive at alkaline ph, escherichia coli must maintain a stable cytoplasmic ph of about 7.6. membrane cation/proton antiporters play a major role in alkaline ph homeostasis by catalyzing active inward proton transport. the deda/tvp38 family is a highly conserved membrane protein family of unknown function present in most sequenced genomes. yqja and yghb are members of the e. col ... | 2015 | 25917916 |
| tick-borne diseases of the usa: ten things clinicians should know. | this article highlights critical aspects of the epidemiology, diagnosis, and management of tick-borne infections in children. principles that apply broadly across the continental united states are emphasized, rather than details of each disease. tick-borne infections are often confused with other, more common childhood illnesses, in part because of their nonspecific initial clinical findings and because patients are usually unaware of their preceding tick exposures. this is a problem, because de ... | 2015 | 25917805 |
| interaction of spirochetes with the host fibrinolytic system and potential roles in pathogenesis. | the pathogenic spirochetes borrelia burgdorferi, b. hermsii, b. recurrentis, treponema denticola and leptospira spp. are the etiologic agents of lyme disease, relapsing fever, periodontitis and leptospirosis, respectively. lyme borreliosis is a multi-systemic disorder and the most prevalent tick-borne disease in the northern hemisphere. tick-borne relapsing fever is persistent in endemic areas worldwide, representing a significant burden in some african regions. periodontal disease, a chronic in ... | 2016 | 25914944 |
| the development and evaluation of a loop-mediated isothermal amplification method for the rapid detection of salmonella enterica serovar typhi. | typhoid fever remains a public health threat in many countries. a positive result in traditional culture is a gold-standard for typhoid diagnosis, but this method is time consuming and not sensitive enough for detection of samples containing a low copy number of the target organism. the availability of the loop-mediated isothermal amplification (lamp) assay, which offers high speed and simplicity in detection of specific targets, has vastly improved the diagnosis of numerous infectious diseases. ... | 2015 | 25910059 |
| the growing spectrum of antibody-associated inflammatory brain diseases in children. | to describe the clinical spectrum, diagnostic evaluation, current management, and neurologic outcome of pediatric antibody-associated inflammatory brain diseases (ab-associated ibraind). | 2015 | 25909091 |
| intracellular concentrations of borrelia burgdorferi cyclic di-amp are not changed by altered expression of the cdaa synthase. | the second messenger nucleotide cyclic diadenylate monophosphate (c-di-amp) has been identified in several species of gram positive bacteria and chlamydia trachomatis. this molecule has been associated with bacterial cell division, cell wall biosynthesis and phosphate metabolism, and with induction of type i interferon responses by host cells. we demonstrate that b. burgdorferi produces a c-di-amp synthase, which we designated cdaa. both cdaa and c-di-amp levels are very low in cultured b. burgd ... | 2015 | 25906393 |
| genome-wide dna methylation analysis of haloferax volcanii h26 and identification of dna methyltransferase related pd-(d/e)xk nuclease family protein hvo_a0006. | restriction-modification (rm) systems have evolved to protect the cell from invading dnas and are composed of two enzymes: a dna methyltransferase and a restriction endonuclease. although rm systems are present in both archaeal and bacterial genomes, dna methylation in archaea has not been well defined. in order to characterize the function of rm systems in archaeal species, we have made use of the model haloarchaeon haloferax volcanii. a genomic dna methylation analysis of h. volcanii strain h2 ... | 2015 | 25904898 |
| supervised resistance exercise for patients with persistent symptoms of lyme disease. | the rapidly increasing incidence of lyme disease has become a serious public health problem. persistent symptoms of lyme disease occur in over 40% of the 300,000 new cases diagnosed annually in the united states and often include debilitating musculoskeletal pain, fatigue, and poor health-related quality of life. no clinical practice guidelines for lyme disease currently include resistance exercise partly because of concern over its safety and feasibility in this population. the goal of this pil ... | 2015 | 25899100 |
| manganese homeostasis and utilization in pathogenic bacteria. | manganese (mn) is a required cofactor for all forms of life. given the importance of mn to bacteria, the host has devised strategies to sequester mn from invaders. in the macrophage phagosome, nramp1 removes mn and other essential metals to starve intracellular pathogens; in the extracellular space, calprotectin chelates mn and zn. calprotectin-mediated mn sequestration is a newly appreciated host defense mechanism, and recent findings are highlighted herein. in order to acquire mn when extracel ... | 2015 | 25898914 |
| potential role of masting by introduced bamboos in deer mice (peromyscus maniculatus) population irruptions holds public health consequences. | we hypothesized that the ongoing naturalization of frost/shade tolerant asian bamboos in north america could cause environmental consequences involving introduced bamboos, native rodents and ultimately humans. more specifically, we asked whether the eventual masting by an abundant leptomorphic ("running") bamboo within pacific northwest coniferous forests could produce a temporary surfeit of food capable of driving a population irruption of a common native seed predator, the deer mouse (peromysc ... | 2015 | 25898267 |
| diaphragm paralysis in lyme disease: late occurrence in the course of treatment and long-term recovery. | 2015 | 25897747 | |
| recent us case of variant creutzfeldt-jakob disease-global implications. | variant creutzfeldt-jakob disease (vcjd) is a rare, fatal prion disease resulting from transmission to humans of the infectious agent of bovine spongiform encephalopathy. we describe the clinical presentation of a recent case of vcjd in the united states and provide an update on diagnostic testing. the location of this patient's exposure is less clear than those in the 3 previously reported us cases, but strong evidence indicates that exposure to contaminated beef occurred outside the united sta ... | 2015 | 25897712 |
| the lyme law. | 2015 | 25897168 | |
| evaluation of the importance of vlse antigenic variation for the enzootic cycle of borrelia burgdorferi. | efficient acquisition and transmission of borrelia burgdorferi by the tick vector, and the ability to persistently infect both vector and host, are important elements for the life cycle of the lyme disease pathogen. previous work has provided strong evidence implicating the significance of the vls locus for b. burgdorferi persistence. however, studies involving vls mutant clones have thus far only utilized in vitro-grown or host-adapted spirochetes and laboratory strains of mice. additionally, t ... | 2015 | 25893989 |
| inflammation in the pathogenesis of lyme neuroborreliosis. | lyme neuroborreliosis, caused by the spirochete borrelia burgdorferi, affects both peripheral and central nervous systems. we assessed a causal role for inflammation in lyme neuroborreliosis pathogenesis by evaluating the induced inflammatory changes in the central nervous system, spinal nerves, and dorsal root ganglia (drg) of rhesus macaques that were inoculated intrathecally with live b. burgdorferi and either treated with dexamethasone or meloxicam (anti-inflammatory drugs) or left untreated ... | 2015 | 25892509 |
| borrelia miyamotoi: a widespread tick-borne relapsing fever spirochete. | borrelia miyamotoi is a relapsing fever spirochete that has only recently been identified as a human pathogen. borrelia miyamotoi is genetically and ecologically distinct from borrelia burgdorferi sensu lato, while both are present in ixodes ticks. over 50 patients with an acute febrile illness have been described with a b. miyamotoi infection, and two infected immunocompromised patients developed a meningoencephalitis. seroprevalence studies indicate exposure in the general population and in sp ... | 2015 | 25892254 |
| immunization with a recombinant subunit ospa vaccine markedly impacts the rate of newly acquired borrelia burgdorferi infections in client-owned dogs living in a coastal community in maine, usa. | in north america, borrelia burgdorferi is the causative bacterial agent of canine lyme borreliosis and is transmitted following prolonged attachment and feeding of vector ticks, ixodes scapularis or ixodes pacificus. its prevention is predicated upon tick-avoidance, effective on-animal tick control and effective immunization strategies. the purpose of this study is to characterize dogs that are newly seropositive for borrelia burgdorferi infection in relation to compliant use of a recombinant os ... | 2015 | 25890386 |
| vertical transmission of bartonella schoenbuchensis in lipoptena cervi. | lipoptena cervi (diptera: hippoboscidae) is a hematophagous ectoparasite of cervids, which is considered to transmit pathogens between animals and occasionally to humans. the principal life stage that is able to parasitize new hosts is a winged ked that just emerged from a pupa. to facilitate efficient transmission of pathogens between hosts, vertical transmission from female deer keds to their offspring is necessary. we investigated vertical transmission of several vector-borne pathogens associ ... | 2015 | 25889985 |
| canine vector-borne infections in mauritius. | canine vector-borne diseases have a worldwide distribution, but to the best of our knowledge, no research has been carried out to evaluate their presence on the indian ocean island of mauritius. an investigation into canine vector-borne infections was conducted in dogs (n = 78) resident at an animal shelter in port louis, mauritius using a combination of traditional microscopy and serological methods. | 2015 | 25889893 |
| inflammatory mediator release from primary rhesus microglia in response to borrelia burgdorferi results from the activation of several receptors and pathways. | in previous studies, neurons were documented to undergo apoptosis in the presence of microglia and live borrelia burgdorferi, but not with either agent alone. microscopy showed that several toll-like receptors (tlrs) were upregulated in microglia upon b. burgdorferi exposure. it was hypothesized that the inflammatory milieu generated by microglia in the presence of b. burgdorferi results in neuronal apoptosis and that this inflammation was likely generated through tlr pathways. | 2015 | 25889406 |
| rhipicephalus microplus and ixodes ovatus cystatins in tick blood digestion and evasion of host immune response. | cystatins are a group of cysteine protease inhibitors responsible for physiological proteolysis regulation and present in a wide range of organisms. studies about this class of inhibitors in parasites have contributed to clarify their roles in important physiological processes, like blood digestion and modulation of host immune response during blood feeding. thus, cystatins are a subject of research on the development of new parasite control methods. additionally, the characterization of protein ... | 2015 | 25889092 |
| physician reported incidence of early and late lyme borreliosis. | lyme borreliosis is the most common vector-borne disease in europe and north america. the objective of this study is to estimate the incidence of tick bites and lyme borreliosis, representative of our entire country, including erythema migrans, disseminated lyme borreliosis and persisting symptoms attributed to lyme borreliosis. | 2015 | 25889086 |
| long-term assessment of health-related quality of life in patients with culture-confirmed early lyme disease. | the health-related quality of life of 100 subjects with culture-confirmed early lyme disease enrolled in a prospective study with annual follow-up visits was evaluated using the 36-item short form general health survey version 2 (sf-36v2) questionnaire at 11-20 years after diagnosis. the mean summary scores of physical and mental health were similar to those of the general population. | 2015 | 25888674 |
| editorial commentary: life after lyme disease. | 2015 | 25888673 | |
| an invasive mammal (the gray squirrel, sciurus carolinensis) commonly hosts diverse and atypical genotypes of the zoonotic pathogen borrelia burgdorferi sensu lato. | invasive vertebrate species can act as hosts for endemic pathogens and may alter pathogen community composition and dynamics. for the zoonotic pathogen borrelia burgdorferi sensu lato, the agent of lyme borreliosis, recent work shows invasive rodent species can be of high epidemiological importance and may support host-specific strains. this study examined the role of gray squirrels (sciurus carolinensis) (n = 679), an invasive species in the united kingdom, as b. burgdorferi sensu lato hosts. w ... | 2015 | 25888168 |
| identification of three extra-chromosomal replicons in leptospira pathogenic strain and development of new shuttle vectors. | the genome of pathogenic leptospira interrogans contains two chromosomes. plasmids and prophages are known to play specific roles in gene transfer in bacteria and can potentially serve as efficient genetic tools in these organisms. although plasmids and prophage remnants have recently been reported in leptospira species, their characteristics and potential applications in leptospiral genetic transformation systems have not been fully evaluated. | 2015 | 25887950 |
| characterization of the β-barrel assembly machine accessory lipoproteins from borrelia burgdorferi. | like all diderm bacteria studied to date, borrelia burgdorferi possesses a β-barrel assembly machine (bam) complex. the bacterial bam complexes characterized thus far consist of an essential integral outer membrane protein designated bama and one or more accessory proteins. the accessory proteins are typically lipid-modified proteins anchored to the inner leaflet of the outer membrane through their lipid moieties. we previously identified and characterized the b. burgdorferi bama protein in deta ... | 2015 | 25887384 |
| u.s. public's experience with ticks and tick-borne diseases: results from national healthstyles surveys. | surveillance data indicate that tick-borne diseases (tbds) are a substantial public health problem in the united states, yet information on the frequency of tick exposure and tbd awareness and prevention practices among the general population is limited. the objective of this study was to gain a more complete understanding of the u.s. public's experience with tbds using data from annual, nationally representative healthstyles surveys. there were 4728 respondents in 2009, 4050 in 2011, and 3503 i ... | 2015 | 25887156 |
| first arrived takes all: inhibitory priority effects dominate competition between co-infecting borrelia burgdorferi strains. | within-host microbial communities and interactions among microbes are increasingly recognized as important factors influencing host health and pathogen transmission. the microbial community associated with a host is indeed influenced by a complex network of direct and indirect interactions between the host and the lineages of microbes it harbors, but the mechanisms are rarely established. we investigated the within-host interactions among strains of borrelia burgdorferi, the causative agent of l ... | 2015 | 25887119 |
| bacterial and protozoal agents of canine vector-borne diseases in the blood of domestic and stray dogs from southern portugal. | the so-called canine vector-borne diseases (cvbd) are caused by a wide range of pathogens transmitted by arthropods. in addition to their veterinary importance, many of these canine vector-borne pathogens can also affect the human population due to their zoonotic potential, a situation that requires a one health approach. as the prevalence of vector-borne pathogens in cats from southern portugal has been recently evaluated, the aim of the present study was to assess if the same agents were prese ... | 2015 | 25886525 |
| rna-seq-based analysis of changes in borrelia burgdorferi gene expression linked to pathogenicity. | lyme disease is a global public health problem caused by the spirochaete borrelia burgdorferi. our previous studies found differences in disease severity between b. burgdorferi b31- and b. garinii sz-infected mice. we hypothesized that genes that are differentially expressed between borrelia isolates encode bacterial factors that contribute to disease diversity. | 2015 | 25886272 |
| presence of zoonotic agents in engorged ticks and hedgehog faeces from erinaceus europaeus in (sub) urban areas. | european hedgehogs (erinaceus europaeus) are hosts for ixodes hexagonus and i. ricinus ticks, which are vectors for zoonotic microorganisms. in addition, hedgehogs may carry several enteric zoonoses as well. it is unclear to what extent a presence of pathogens in hedgehogs poses a risk to public health, as information on the presence of zoonotic agents in hedgehogs in urban areas is relatively scarce. | 2015 | 25885888 |
| prevalence of borrelia burgdorferi-infected ticks from wildlife hosts, a response to norris et al. | in a recent letter to the editor, norris et al. questioned the validity of some of our data reported by feria-arroyo et al. the main issue investigated by us was the potential impact of climate change on the probable distribution of the tick vector ixodes scapularis in the texas-mexico transboundary region. as an ancillary issue, an analysis of sequence data for the intergenic spacer of borrelia burgdorferi was conducted. in the present letter, we provide further evidence supporting our original ... | 2015 | 25885773 |
| efficacy of a fixed combination of permethrin 54.5% and fipronil 6.1% (effitix) in dogs experimentally infested with ixodes ricinus. | ticks are the most important vectors of disease-causing pathogens in domestic animals and are considered to be second worldwide to mosquitoes as vectors of human diseases. in europe, ixodes ricinus, the sheep tick, plays an important role as companion animal parasite but is also the primary vector of medically important diseases such as tick-borne encephalitis and lyme borreliosis. the present study was designed to evaluate the efficacy under laboratory conditions of a new fixed spot-on combinat ... | 2015 | 25884999 |
| development of dirofilaria immitis within the mosquito aedes (finlaya) koreicus, a new invasive species for europe. | over the recent decades, container-breeding mosquito species belonging to the genus aedes have frequently been recorded far from their place of origin. aedes koreicus was first reported in north-eastern italy in 2011, in a region endemic for dirofilaria immitis, the agent of canine heartworm disease. the vector competence of ae. koreicus for d. immitis was here tested under laboratory conditions, by infecting mosquitoes with a local strain of d. immitis. | 2015 | 25884876 |
| factors associated with preventive behaviors regarding lyme disease in canada and switzerland: a comparative study. | lyme disease (ld) is a vector-borne disease that is endemic in many temperate countries, including switzerland, and is currently emerging in canada. this study compares the importance of knowledge, exposure and risk perception for the adoption of individual preventive measures, within and between two different populations, one that has been living in a ld endemic region for several decades, the neuchâtel canton in switzerland, and another where the disease is currently emerging, the montérégie r ... | 2015 | 25884424 |
| lyme disease: recent advances and perspectives. | 2015 | 25883907 | |
| glyphosate, pathways to modern diseases iii: manganese, neurological diseases, and associated pathologies. | manganese (mn) is an often overlooked but important nutrient, required in small amounts for multiple essential functions in the body. a recent study on cows fed genetically modified roundup(®)-ready feed revealed a severe depletion of serum mn. glyphosate, the active ingredient in roundup(®), has also been shown to severely deplete mn levels in plants. here, we investigate the impact of mn on physiology, and its association with gut dysbiosis as well as neuropathologies such as autism, alzheimer ... | 2015 | 25883837 |
| unilateral epiphora in an adolescent. | 2014 | 25883767 | |
| lyme disease presenting as multiple ischaemic strokes. | a 46-year-old man presented with recurrent left hemiparesis and headache. mri of brain showed an acute right pontine and subacute right thalamic infarcts and mr angiogram showed multiple intracranial arterial stenoses, suggesting cerebral vasculopathy. there was a cerebrospinal fluid lymphocytic pleocytosis with borrelia burgdorferi antibodies. central nervous system lyme disease occasionally presents with ischaemic strokes; this case is unusual in showing vasculopathy on brain imaging, supporti ... | 2015 | 25882056 |
| vector potential and population dynamics for amblyomma inornatum. | we studied the natural life cycle of amblyomma inornatum and its vector potential in south texas. this tick is distributed throughout south texas and most of central america. a. inornatum represented 1.91% of the ticks collected by carbon dioxide traps during a study of free-living ticks in the tamaulipan biotic province in south texas. the life cycle of a. inornatum in south texas showed a clear seasonal pattern consistent with one generation per year. nymphs emerged in the spring with a peak i ... | 2015 | 25881916 |
| progressive hemifacial atrophy: a review. | progressive hemifacial atrophy (pha) is an acquired, typically unilateral, facial distortion with unknown etiology. the true incidence of this disorder has not been reported, but it is often regarded as a subtype of localized scleroderma. historically, a debate existed whether pha is a form of linear scleroderma, called morphea en coup de sabre (ecds), or whether these conditions are inherently different processes or appear on a spectrum (; adv exp med biol 455:101-4, 1999; j eur acad dermatol v ... | 2015 | 25881068 |
| exploring the association between morgellons disease and lyme disease: identification of borrelia burgdorferi in morgellons disease patients. | morgellons disease (md) is a complex skin disorder characterized by ulcerating lesions that have protruding or embedded filaments. many clinicians refer to this condition as delusional parasitosis or delusional infestation and consider the filaments to be introduced textile fibers. in contrast, recent studies indicate that md is a true somatic illness associated with tickborne infection, that the filaments are keratin and collagen in composition and that they result from proliferation and activa ... | 2015 | 25879673 |
| extensive infectious myelitis post bariatric surgery. | inflammatory myelopathy is an inflammatory neurological disorder of the spinal cord (myelopathy). it occurs in 1 (severe) to 8 (mild) cases/million per year. it is often referred to in the literature as "transverse myelitis" or "acute transverse myelitis". myelopathy and by extension myelitis, can present as pyramidal (motor), sensory, and/or autonomic dysfunction to varying degrees. symptoms typically develop over hours to days and worsen over days to weeks. sensory symptoms usually present as ... | 2015 | 25879204 |
| oral manifestations of parry-romberg syndrome: a review of literature. | parry-romberg syndrome (prs) or progressive facial hemiatrophy is a developmental craniofacial disorder of unknown etiology characterized by a slowly progressive unilateral facial atrophy. it is associated with different systemic manifestations particularly, maxillofacial, neurologic and ophthalmologic abnormalities. dentists must be aware of prs to identify this invalidating disorder. in this article, we review the etiology, clinical features (especially craniofacial and dental manifestations) ... | 2015 | 25878963 |
| acute diffuse and total alopecia of the female scalp associated with borrelia-infection. | a case of acute diffuse and total alopecia of the female scalp associated with borrelia-infection (acrodermatitis chronica atrophicans) is presented. today, acute diffuse and total alopecia of the female scalp is recognized as a distinct variant of alopecia areata (aa) predominantly observed in women. cases of aa have formerly been reported in association with infections. aa is understood to represent an organ-specific autoimmune disease of the hair follicle. it is conceivable that the antigenic ... | 2015 | 25878446 |
| identification of a core sequence for the binding of bosr to the rpos promoter region in borrelia burgdorferi. | 2015 | 25877426 | |
| popliteal cyst with positive antinuclear antibodies as the presentation of lyme disease. | 2015 | 25876095 | |
| phylogenomic identification of regulatory sequences in bacteria: an analysis of statistical power and an application to borrelia burgdorferi sensu lato. | phylogenomic footprinting is an approach for ab initio identification of genome-wide regulatory elements in bacterial species based on sequence conservation. the statistical power of the phylogenomic approach depends on the degree of sequence conservation, the length of regulatory elements, and the level of phylogenetic divergence among genomes. building on an earlier model, we propose a binomial model that uses synonymous tree lengths as neutral expectations for determining the statistical sign ... | 2015 | 25873371 |
| procoagulant microparticles in dogs with immune-mediated hemolytic anemia. | studies of some human prothrombotic diseases suggest that phosphatidylserine-positive (ps+) and tissue factor-positive (tf+) microparticles (mps) might play a role in the pathogenesis of thrombosis or serve as biomarkers of thrombotic risk. | 2015 | 25871966 |
| human granulocytic anaplasmosis in the united states from 2008 to 2012: a summary of national surveillance data. | human granulocytic anaplasmosis is an acute, febrile illness transmitted by the ticks ixodes scapularis and ixodes pacificus in the united states. we present a summary of passive surveillance data for cases of anaplasmosis with onset during 2008-2012. the overall reported incidence rate (ir) was 6.3 cases per million person-years. cases were reported from 38 states and from new york city, with the highest incidence in minnesota (ir = 97), wisconsin (ir = 79), and rhode island (ir = 51). thirty-s ... | 2015 | 25870428 |