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primary amyloid polyneuropathy with myocardial uptake of technetium-99m-diphosphonate. 20103030187
the chicken carbonic anhydrase ii gene: evidence for a recent shift in intron position.the complete nucleotide sequence of the coding region of the chicken carbonic anhydrase ii (ca ii) gene has been determined from clones isolated from a chicken genomic library. the sequence of a nearly full length chicken ca ii cdna clone has also been obtained. the gene is approximately 17 kilobase pairs (kb) in size and codes for a protein that is comprised of 259 amino acid residues. the 5' flanking region contains consensus sequences commonly associated with eucaryotic genes transcribed by r ...19873029691
pleomorphic adenoma of skin (chondroid syringoma). 20103028949
lung cancer in northern india in relation to age, sex and smoking habits.primary lung cancer in northern india was analysed in relation to age, sex and smoking history in 480 histologically diagnosed cases. the m:f ratio rose progressively up to the 51-60 years age group and was about the same thereafter. the m:f ratio was very high in smokers and was almost identical for all cell types (greater than 20:1). in non-smokers, the m:f ratio differed with the cell type. the smoker:non-smoker ratio differed significantly in the age groups below and above 40. in those up to ...20103028854
lung cancer in northern india in relation to age, sex and smoking habits.primary lung cancer in northern india was analysed in relation to age, sex and smoking history in 480 histologically diagnosed cases. the m:f ratio rose progressively up to the 51-60 years age group and was about the same thereafter. the m:f ratio was very high in smokers and was almost identical for all cell types (greater than 20:1). in non-smokers, the m:f ratio differed with the cell type. the smoker:non-smoker ratio differed significantly in the age groups below and above 40. in those up to ...20103028854
interaction of kappa receptor agonists with ca2+ channel antagonists in the modulation of hypothermia.administration of the opiate u-50,488h (3-20 mg/kg s.c.), a selective kappa receptor agonist, produced a dose-dependent decrease of rectal temperature in rats. this hypothermic effect of u-50,488h was accompanied by an enhanced activity of ca2+/mg2+ atpase in crude synaptosomal (p2) fractions obtained from hypothalamus but not from cortex or cerebellum. mg2+ atpase activity in these regions was not altered by u-50,488h (15 mg/kg s.c.). naloxone (5 mg/kg) partially and mr2266 (5 mg/kg) completely ...19863028838
interaction of kappa receptor agonists with ca2+ channel antagonists in the modulation of hypothermia.administration of the opiate u-50,488h (3-20 mg/kg s.c.), a selective kappa receptor agonist, produced a dose-dependent decrease of rectal temperature in rats. this hypothermic effect of u-50,488h was accompanied by an enhanced activity of ca2+/mg2+ atpase in crude synaptosomal (p2) fractions obtained from hypothalamus but not from cortex or cerebellum. mg2+ atpase activity in these regions was not altered by u-50,488h (15 mg/kg s.c.). naloxone (5 mg/kg) partially and mr2266 (5 mg/kg) completely ...19863028838
[aspergillosis following liver transplantation as a hospital infection].in the first four weeks after a liver transplantation, there was an invasive aspergillosis with a lethal course in three out of five patients who were treated postoperatively in the same room. the clinical symptoms were very different. one patient was asymptomatic, and the diagnosis could only be made by autopsy. in another patient, pulmonary symptoms, and in the third patient, cerebral symptoms were the most prominent. in the two latter patients, the infection was demonstrated in the sputum and ...19873028750
[aspergillosis following liver transplantation as a hospital infection].in the first four weeks after a liver transplantation, there was an invasive aspergillosis with a lethal course in three out of five patients who were treated postoperatively in the same room. the clinical symptoms were very different. one patient was asymptomatic, and the diagnosis could only be made by autopsy. in another patient, pulmonary symptoms, and in the third patient, cerebral symptoms were the most prominent. in the two latter patients, the infection was demonstrated in the sputum and ...19873028750
t-lymphocytes subpopulation before and after surgical removal of hepatocellular carcinoma.this study included 16 histologically confirmed hepato-cellular carcinoma (hcc) patients. at the same time, 32 normal healthy individuals and 20 asymptomatic hepatitis b surface antigen (hbsag) carriers were served as control groups. the study disclosed that t cells, okt4 cells and the ratio of okt4 cells/okt8 cells in peripheral venous blood of hcc patients before surgery were lower than that of either normal control or asymptomatic hbsag carriers. the difference was statistically significant. ...20103028718
t-lymphocytes subpopulation before and after surgical removal of hepatocellular carcinoma.this study included 16 histologically confirmed hepato-cellular carcinoma (hcc) patients. at the same time, 32 normal healthy individuals and 20 asymptomatic hepatitis b surface antigen (hbsag) carriers were served as control groups. the study disclosed that t cells, okt4 cells and the ratio of okt4 cells/okt8 cells in peripheral venous blood of hcc patients before surgery were lower than that of either normal control or asymptomatic hbsag carriers. the difference was statistically significant. ...20103028718
bone and gallium scan findings in malignant fibrous histiocytoma. case report with radiographic and pathologic correlation.malignant fibrous histiocytoma (mfh) is the most common soft tissue malignancy in adults. the ga-67 citrate scan findings of an extremity-located mfh, the most common location of this neoplasm, have never been published in english language journals to the best of the authors' knowledge. ga-67 citrate and tc-99m mdp scans of the thigh mass accurately depicted the tumor's local extent, including the presence of central ischemic necrosis within the tumor, and the absence of adjacent osseous involve ...20103028689
bone and gallium scan findings in malignant fibrous histiocytoma. case report with radiographic and pathologic correlation.malignant fibrous histiocytoma (mfh) is the most common soft tissue malignancy in adults. the ga-67 citrate scan findings of an extremity-located mfh, the most common location of this neoplasm, have never been published in english language journals to the best of the authors' knowledge. ga-67 citrate and tc-99m mdp scans of the thigh mass accurately depicted the tumor's local extent, including the presence of central ischemic necrosis within the tumor, and the absence of adjacent osseous involve ...20103028689
neutrophil dysfunction. case studies and review. 20103028674
neutrophil dysfunction. case studies and review. 20103028674
peripheral dtic neurotoxicity: a case report. 20103028665
peripheral dtic neurotoxicity: a case report. 20103028665
tifluadom, a kappa-opiate agonist, acts as a peripheral cholecystokinin receptor antagonist.tifluadom, a benzodiazepine kappa-opiate agonist, stereoselectively inhibited the binding of 125i-cck to pancreatic membranes (ic50 = 47 nm). several other opiate agonists were ineffective. scatchard analysis indicated the inhibition of cck binding by tifluadom was competitive in nature. tifluadom (1 microm) did not displace 125i-cck binding to brain tissue or 125i-gastrin binding to fundic glands. in the isolated guinea pig gallbladder, tifluadom antagonized cck-8 induced contractions with an e ...19863027627
gene expression and genetic engineering in the lens. friedenwald lecture. 19873026987
gene expression and genetic engineering in the lens. friedenwald lecture. 19873026987
[virus-related gastroenteritis]. 19873026769
[transient hiv-antibody positive tests in heart transplantation patients after repeated administration of a cmv immune globulin preparation].after repeated administration of relatively high doses of a cytomegalovirus immunoglobulin preparation to two patients after heart transplantation, both the elisa and the immuno-blot tests became temporarily positive (for 21 and 41 days, respectively) to hiv antibodies. the cmv immunoglobulin preparation was demonstrated to have high hiv antibody titres. the latter ran parallel to antibody titres against measles virus, also transferred passively with the immunoglobulin preparation. this makes it ...19873026767
[intrauterine fetal death following erythema infectiosum]. 20113026764
phenotypic characterisation of peripheral blood lymphoid cells in people exposed to fibrous zeolite.among inhabitants of the village of karain in turkey there is an extremely high incidence of malignant mesothelioma, most probably due to exposure to erionite, which is a fibrous zeolite and similar in appearance and properties to asbestos. this mineral may be found in the dust in the village. to characterise possible disturbances in the immune system of people exposed to fibrous zeolite, a phenotypic characterisation of lymphoid cells in the peripheral blood of 74 immigrants to sweden from kara ...19863026434
phenotypic characterisation of peripheral blood lymphoid cells in people exposed to fibrous zeolite.among inhabitants of the village of karain in turkey there is an extremely high incidence of malignant mesothelioma, most probably due to exposure to erionite, which is a fibrous zeolite and similar in appearance and properties to asbestos. this mineral may be found in the dust in the village. to characterise possible disturbances in the immune system of people exposed to fibrous zeolite, a phenotypic characterisation of lymphoid cells in the peripheral blood of 74 immigrants to sweden from kara ...19863026434
diacylglycerol lipase and kinase activities in rabbit aorta and coronary microvessels.diacylglycerol lipase and kinase activities were measured in particulate and soluble fractions from rabbit aorta (intima-media) and coronary microvessels. with rabbit aorta, the hydrolysis at the sn-1 position of 1-palmitoyl-2-oleoyl-sn-glycerol had a ph optimum of 5-6 and was greater than hydrolysis at the sn-2 position (ph optimum of 6.5). only the 2-monoacylglycerol accumulated during incubations at ph 5 and 6.5. these results are consistent with an ordered two-step reaction sequence where th ...19863026422
diacylglycerol lipase and kinase activities in rabbit aorta and coronary microvessels.diacylglycerol lipase and kinase activities were measured in particulate and soluble fractions from rabbit aorta (intima-media) and coronary microvessels. with rabbit aorta, the hydrolysis at the sn-1 position of 1-palmitoyl-2-oleoyl-sn-glycerol had a ph optimum of 5-6 and was greater than hydrolysis at the sn-2 position (ph optimum of 6.5). only the 2-monoacylglycerol accumulated during incubations at ph 5 and 6.5. these results are consistent with an ordered two-step reaction sequence where th ...19863026422
[rare anomalies of the architecture of the bronchial tree].six cases of rare bronchial anomalies are presented (3 complete right-sided hypo-arterial bronchial distributions, 1 partial right-sided hypo-arterial bronchial supply of the upper lobe, 2 cases of atresia of the left apico-posterior bronchus). emphasis is placed on the bronchographic elucidation of the changed bronchial segmental topic if additive or subtractive bronchial anomalies are found endoscopically especially with regard to preoperative aspects.20113025954
diacylglycerol-induced translocation of diacylglycerol kinase: use of affinity-purified enzyme in a reconstitution system.diacylglycerol-induced translocation of diacylglycerol kinase (atp:1,2-diacylglycerol 3-phosphotransferase, ec 2.7.1.107) from the soluble to the membrane-bound compartments was demonstrated both in crude tissue homogenates and in a reconstituted enzyme-membrane model system. in homogenates of either rat brain or liver, incubation with diacylglycerol or phospholipase c, but not phospholipase a2 or phospholipase d, resulted in the translocation of diacylglycerol kinase activity from the soluble t ...19863025839
identification of a conserved domain among cyclic nucleotide phosphodiesterases from diverse species.partial amino acid sequences have been determined for the ca2+/calmodulin-stimulated cyclic nucleotide phosphodiesterase from bovine brain and the cgmp-stimulated cyclic nucleotide phosphodiesterase from bovine heart. examination of these sequences for homologous segments and comparison with protein sequences derived from the nucleotide sequences of the yeast pde2 gene and the drosophila dunce+ gene [chen, c.-n., denome, s. & davis, r. l. (1986) proc. natl. acad. sci. usa 83, 9313-9317; sass, p. ...19863025833
effect of ketoconazole on placental aromatase, 3 beta-hydroxysteroid dehydrogenase-isomerase and 17 beta-hydroxysteroid dehydrogenase.ketoconazole, an orally-active, broad spectrum mycotic agent, was shown to inhibit in vitro human placental microsomal aromatase but was without effect on 3 beta-hydroxysteroid dehydrogenase-isomerase (3 beta-hsd-i) and 17 beta-hydroxysteroid dehydrogenase (17 beta-hsd) activities. the km of placental aromatase for testosterone was 30 +/- 1.1 nmol/l (mean +/- sem, n = 6). inhibition (determined by lineweaver-burk plot) was non-competitive with respect to substrate with a ki value of 3.0 +/- 1.4 ...19863025521
studies on the vibrio cholerae mucinase complex. i. enzymic activities associated with the complex.mucinase enzymes were isolated and partially purified from the culture fluid of vibrio cholerae grown in proteose peptone-colostrum medium. the mucinase complex contained neuraminidase, endo-beta-n-acetylhexosaminidase, nicotinamide-adenine-dinucleotidase and proteinases. traces of phospholipase activity were detected but the complex lacked aldolase activity.19863025445
degenerated muscle grafts used for peripheral nerve repair in primates.the basement membrane matrix of skeletal muscle has a tubular configuration resembling that of peripheral nerves. grafts made of autogenous skeletal muscle denatured by freezing and thawing were used to repair the ulnar nerve in marmosets. by six months, normal hand function had returned and the grafts were shown to transmit normal compound extracellular action potentials in both directions. morphological examination of the grafts and distal nerves revealed normal axon numbers and axon maturity. ...19863025319
molecular epidemiology of bacterial infections: examples of methodology and of investigations of outbreaks.plasmid profile analysis, plasmid and chromosomal bacterial restriction endonuclease dna analysis, and dna hybridization are increasingly used in clinical microbiology and epidemiology. these techniques have been applied, singly and in combination, to investigations of outbreaks, including those of diarrheal diseases caused by pandemic vibrio cholerae, enterotoxigenic and enterohemorrhagic escherichia coli, salmonella muenchen, and salmonella typhimurium. the techniques have been critical in the ...19863024288
[composites--state of the art]. 20113024214
[the nature of rsi sequences flanking the vct gene encoding the synthesis of cholera toxin in vibrio cholerae eltor]. 19863023983
stereoselectivity of a potent calcium antagonist, 1-benzyl-3-pyrrolidinyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate.four enantiomers (3a-d) of the title compound, ym-09730 (3), were synthesized by the reaction of (-)- or (+)-5-(methoxycarbonyl)-2, 6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3-carboxylic acid (1a or 1b) with (s)- or (r)-1-benzyl-3-pyrrolidinol (2a or 2b). [3h]nitrendipine binding affinity and coronary vasodilating activity of these compounds were evaluated. the absolute configuration of the most potent enantiomer (3a) with the longest duration was unequivocally determined to be (s)-1,4-di ...19863023614
immunosuppressive properties of human placenta: study of supernatants from short-term syncytiotrophoblast cultures.using collagenase and mechanical treatment to attempt to eliminate cellular contamination such as macrophages and decidual cells, trophoblast enriched cell suspensions were isolated from the human placenta. with a view to assessing the role of trophoblast in impairing maternal rejection of the fetus, supernatants (spl4) were prepared from these placental cells after short-term culture (4 h). the immunosuppressive activity of these supernatants was studied following application to mitogen-stimula ...19863023607
detection of dihydroorotate dehydrogenase activity as a marker of de novo nucleic acid synthesis in germinal centers of spleens following antigen administration.a histochemical technique, developed for the localization of dho-d activity in tissue, has been utilized to study the distribution of this activity in rabbit spleen. this enzyme is involved in the de novo synthesis of pyrimidines of nucleic acids via orotic acid as an intermediate. the enzyme was found to be most active at the periphery of follicles in the resting spleen. following antigenic challenge, enzyme activity became prominent for several days in macrophages within germinal centers, but ...19853023585
fk 33-824, a met-enkephalin analog, blocks corticotropin-releasing hormone-induced adrenocorticotropin secretion in normal subjects but not in patients with cushing's disease.to further elucidate the site of action of opioids on the pituitary-adrenal axis, we studied the effect of d-ala2,mephe4,met-(o)enkephalin-ol (sandoz, fk 33-824) on plasma acth and beta-endorphin immunoreactivity and serum cortisol in 7 normal subjects and 11 patients with cushing's syndrome (cushing's disease, n = 7; adrenal adenoma, n = 2; ectopic cushing's syndrome, n = 2) after administration of human corticotropin-releasing hormone (hcrh). hcrh (0.1 mg; bachem) was injected iv after pretrea ...19863023423
cross-linking of a growth hormone releasing factor-binding protein in anterior pituitary cells.growth hormone-releasing factor (grf) stimulates the release of growth hormone from the anterior pituitary and is related to the peptides of the glucagon/secretin family. although the mechanism of action of this hormone has been studied in considerable detail, little is known concerning the grf receptor itself. we have attempted to label the grf receptor by chemically coupling the 125i-grf analog [his1, nle27]-hgrf(1-32)-nh2 (grfa) (where nle is norleucine) to plated rat anterior pituitary cells ...19863023363
cross-linking of a growth hormone releasing factor-binding protein in anterior pituitary cells.growth hormone-releasing factor (grf) stimulates the release of growth hormone from the anterior pituitary and is related to the peptides of the glucagon/secretin family. although the mechanism of action of this hormone has been studied in considerable detail, little is known concerning the grf receptor itself. we have attempted to label the grf receptor by chemically coupling the 125i-grf analog [his1, nle27]-hgrf(1-32)-nh2 (grfa) (where nle is norleucine) to plated rat anterior pituitary cells ...19863023363
immortalization of early embryonic cell derivatives after the transfer of the early region of simian virus 40 into f9 teratocarcinoma cells.f9 embryonal carcinoma (ec) cells were transformed using a recombinant plasmid carrying simian virus 40 (sv40) early genes linked to the promoter-enhancer region of the early transcription unit 1a (e1a) of adenovirus type 5. one clone of transformed cells, f9-k4b2, contained several integrated copies of the plasmid and expressed sv40 early genes (t antigens) only when it was induced to differentiate in vitro by the addition of retinoic acid and dibutyryl-camp. from the cell population positive f ...19863023162
a use for principal coordinate analysis in the comparison of protein sequences.principal coordinate analysis (pco) was applied to the comparison of protein sequences. a similarity matrix was derived from a dataset containing 21 c-type cytochrome sequences and this was analysed using pco to produce a plot of the first three principal axes. the relationships indicated from this plot are considered in conjunction with those derived by cluster analysis using the upgma method, and the advantages offered by a nonhierarchical method of sequence comparison discussed.20113022735
identification and characterization of mg2+-dependent phosphotyrosyl protein phosphatase from rat liver cytosol.although highly purified preparations of mg2+-dependent phosphoseryl protein phosphatase (also designated phosphatase ia or phosphatase 2c) dephosphorylated phosphotyrosyl histone, the activity has been resolved from phosphatase ia by polyacrylamide gel electrophoresis at ph 9.5. this novel phosphotyrosyl-specific protein phosphatase absolutely requires mg2+ or mn2+ for activity, is inhibited by zn2+, vanadate and fluoride, and has an optimal ph of 9.0 and mr = 50,000. certain properties of this ...19863022716
thymidine kinase with altered substrate specificity of acyclovir resistant varicella-zoster virus.the acyclovir resistant mutant of varicella-zoster virus acv-r (a 8) induced the same level of thymidine kinase activity in infected cells as the parent kawaguchi strain. however, it induced less deoxycytidine kinase activity and did not induce phosphorylating activity for the nucleotide analogue, 9-(2 hydroxy-ethoxymethyl)-guanine-(acyclovir). another acyclovir resistant mutant, acv-r (a 4), which is cross-resistant to phosphonoacetate and is thought to be a viral dna polymerase mutant, induced ...19863022711
hemolysin production and cloning of two hemolysin determinants from classical vibrio cholerae.the hemolytic activity of 20 classical and 3 el tor strains of v. cholerae o1 was examined phenotypically and genetically. the el tor strains lysed bovine, chicken, human, rabbit, and sheep erythrocytes (rbcs), while the classical strains lysed only chicken and rabbit rbcs. the assay was done with rbcs in tris-nacl buffer, since phosphate-buffered saline was found to inhibit hemolytic activity. hemolytic activity in culture supernatants from el tor strains was more sensitive to heat inactivation ...19863021628
spermine antagonises the effects of dexamethasone, glucagon and cyclic amp in increasing the activity of phosphatidate phosphohydrolase in isolated rat hepatocytes.rat hepatocytes were incubated in monolayer culture, under serum free conditions, for 8 h. glucagon (10 nm), 8-(4-chlorophenylthio)adenosine 3',5'-cyclic monophosphate (100 microm) and dexamethasone (100 nm) increased the activity of phosphatidate phosphohydrolase by approx. 2-, 3.6- and 3.3-fold, respectively. spermine alone had no significant effect. spermine (2.5 mm) almost completely inhibited the glucagon induced increase in phosphohydrolase activity. it only partially inhibited the dexamet ...19863021529
the o2- -forming nadph oxidase of the phagocytes: nature, mechanisms of activation and function. 20103021215
the o2- -forming nadph oxidase of the phagocytes: nature, mechanisms of activation and function. 20103021215
structure and activities of a variant ubiquitin sequence from bakers' yeast.ubiquitin is an extremely conserved protein, with an identical sequence throughout the animal kingdom. however, the gene sequence of the yeast protein [ozkaynak, e., finley, d., & varshavsky, a. (1984) nature (london) 312, 663-666] predicts three amino acid differences. this implies that some functions or binding interactions of ubiquitin are different in yeast and animal cells. in an effort to define these differences, ubiquitin has been purified to homogeneity from bakers' yeast and characteri ...19863021209
dna distributions in human normal, precancerous and cancerous breast tissue. i. ploidy and cell cycle distribution.dna distributions were recorded flow cytometrically in 19 normal, 23 precancerous tissues with epithelial proliferation and cell atypia (p1), 7 carcinomata lobularia in situ and intraductale carcinomas (p2) and in 44 invasive carcinomas of the human breast. the results were compared to histological findings, tumor staging and grading, lymph node involvement and age of the patients. dna diploidy was found in all normal, 21 p1 tissues, 6 p2 tissues and 12 carcinomas; dna aneuploidy in 2 p1 tissues ...19863021084
dna distributions in human normal, precancerous and cancerous breast tissue. i. ploidy and cell cycle distribution.dna distributions were recorded flow cytometrically in 19 normal, 23 precancerous tissues with epithelial proliferation and cell atypia (p1), 7 carcinomata lobularia in situ and intraductale carcinomas (p2) and in 44 invasive carcinomas of the human breast. the results were compared to histological findings, tumor staging and grading, lymph node involvement and age of the patients. dna diploidy was found in all normal, 21 p1 tissues, 6 p2 tissues and 12 carcinomas; dna aneuploidy in 2 p1 tissues ...19863021084
subtypes and functions of alpha-adrenoceptors. 19863020944
pyruvate kinase inhibition in the diagnosis of gliomas with an intermediate degree of malignancy.the aim of the investigation was to see if the histological diagnosis of brain tumors showing an intermediate degree of malignancy can be improved by the measurement of l-alpha-alanine inhibition of pyruvate kinase isoenzymes. the inhibition of pyruvate kinase activity was measured in 51 gliomas with different grades of malignancy. it was confirmed that benign tumors have a low level of inhibition (less than 50%) and that the more malignant the tumor, the higher the level of inhibition became, r ...20103020861
[possible participation of lipids in information gathering and the amplification of biological signals]. 20103020846
[acute adrenal cortex insufficiency]. 20103020813
[differential diagnosis of viral diarrheas in calves].there is a description of the enzymoimmunologic method (elisa), which was used for demonstration of rotaviruses and coronaviruses in the samples of excrements of the calves suffering from diarrheas. it is shown by a comparison with the results obtained by electron microscopy that the elisa method provides by up to 50% higher capture rate and the reaction is highly specific. the method can also be applied to a detection of human rotavirus in the children's stool.19863020762
[differential diagnosis of viral diarrheas in calves].there is a description of the enzymoimmunologic method (elisa), which was used for demonstration of rotaviruses and coronaviruses in the samples of excrements of the calves suffering from diarrheas. it is shown by a comparison with the results obtained by electron microscopy that the elisa method provides by up to 50% higher capture rate and the reaction is highly specific. the method can also be applied to a detection of human rotavirus in the children's stool.19863020762
exposure of sheep to natural aerosols of foot-and-mouth disease virus.sheep taken individually and allowed to inhale air being drawn along a duct from a cabinet containing pigs acutely infected with foot-and-mouth disease virus for 10 or 15 minute periods were infected by doses as low as 10 tcid50 of virus. the most consistent and reliable indicators of infection were viraemia and seroconversion. the mean times from exposure to onset of viraemia, pyrexia and the appearance of vesicular lesions were 2.5, 3.8 and 4.7 days, respectively. neither the time from exposur ...19863020658
evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ...19863020551
evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ...19863020551
[kinin aminopeptidase in lung tissue and activity of kininase ii in the serum of patients with silicosis]. 20103020528
[kinin aminopeptidase in lung tissue and activity of kininase ii in the serum of patients with silicosis]. 20103020528
carbachol- and norepinephrine-stimulated phosphoinositide metabolism in rat brain: effect of chronic cholinesterase inhibition.activation of cholinergic muscarinic receptors leads to several biochemical events including an increased turnover of phosphoinositides. in this study we have investigated whether repeated administration of the organophosphorus insecticide disulfoton, known to cause the development of tolerance to this compound, would affect phosphoinositide metabolism in rat brain. basal and carbachol-stimulated phosphoinositide metabolism were measured in cerebral cortex slices, by measuring the accumulation o ...19863020234
carbachol- and norepinephrine-stimulated phosphoinositide metabolism in rat brain: effect of chronic cholinesterase inhibition.activation of cholinergic muscarinic receptors leads to several biochemical events including an increased turnover of phosphoinositides. in this study we have investigated whether repeated administration of the organophosphorus insecticide disulfoton, known to cause the development of tolerance to this compound, would affect phosphoinositide metabolism in rat brain. basal and carbachol-stimulated phosphoinositide metabolism were measured in cerebral cortex slices, by measuring the accumulation o ...19863020234
[effect of arterial injection of lipiodol-adriamycin emulsion on hepatocellular carcinoma]. 20063020138
hepatocellular adenoma and nodular regenerative hyperplasia of the liver in a young man.a 26-year-old man had a massive intraabdominal hemorrhage from a hepatocellular adenoma (hca). the tumor arose within a liver that demonstrated generalized nodular regenerative hyperplasia. the patient had no factors predisposing to either hca or nodular regenerative hyperplasia (nrh) of the liver. although rare, hca should be included in the differential diagnosis of spontaneous intraperitoneal hemorrhage even in young men. the coexistence of hca and nrh of the liver in this patient may indicat ...19863020120
hepatocellular adenoma and nodular regenerative hyperplasia of the liver in a young man.a 26-year-old man had a massive intraabdominal hemorrhage from a hepatocellular adenoma (hca). the tumor arose within a liver that demonstrated generalized nodular regenerative hyperplasia. the patient had no factors predisposing to either hca or nodular regenerative hyperplasia (nrh) of the liver. although rare, hca should be included in the differential diagnosis of spontaneous intraperitoneal hemorrhage even in young men. the coexistence of hca and nrh of the liver in this patient may indicat ...19863020120
deletion of exon encoding cysteine-rich repeat of low density lipoprotein receptor alters its binding specificity in a subject with familial hypercholesterolemia.the proposed ligand binding domain of the low density lipoprotein (ldl) receptor consists of a 40-amino acid cysteine-rich unit that is repeated with some variation seven times. we describe here a mutant allele at the ldl receptor locus in which one of the seven repeats has been deleted. this mutation was found in a patient with the clinical syndrome of homozygous familial hypercholesterolemia. by molecular cloning, we show that the deletion arose by homologous recombination between repetitive a ...20103020025
deletion of exon encoding cysteine-rich repeat of low density lipoprotein receptor alters its binding specificity in a subject with familial hypercholesterolemia.the proposed ligand binding domain of the low density lipoprotein (ldl) receptor consists of a 40-amino acid cysteine-rich unit that is repeated with some variation seven times. we describe here a mutant allele at the ldl receptor locus in which one of the seven repeats has been deleted. this mutation was found in a patient with the clinical syndrome of homozygous familial hypercholesterolemia. by molecular cloning, we show that the deletion arose by homologous recombination between repetitive a ...20103020025
phase i/ii trials of wr-2721 and cis-platinum.renal dysfunction is a well-known dose-limiting toxicity of cis-platinum. previous studies demonstrated a 32% incidence of nephrotoxicity following a 100 mg/m2 single dose of cis-platinum with mannitol diuresis. wr-2721 is an aminothiol which in the animal model improves renal tolerance to cis-platinum by factors of 1.3 to 1.7. phase i trials were initiated to establish the toxicity when wr-2721 was given prior to escalating doses of cis-platinum. fifty-two patients received 161 courses of wr-27 ...19863019969
phase i/ii trials of wr-2721 and cis-platinum.renal dysfunction is a well-known dose-limiting toxicity of cis-platinum. previous studies demonstrated a 32% incidence of nephrotoxicity following a 100 mg/m2 single dose of cis-platinum with mannitol diuresis. wr-2721 is an aminothiol which in the animal model improves renal tolerance to cis-platinum by factors of 1.3 to 1.7. phase i trials were initiated to establish the toxicity when wr-2721 was given prior to escalating doses of cis-platinum. fifty-two patients received 161 courses of wr-27 ...19863019969
an improved synthesis of (3-n-[11c]methyl)spiperone.an improved radiochemical synthesis of (3-n-[11c]methyl) spiperone is reported. the radiotracer was prepared by n-alkylation of spiperone in dimethylformamide in the presence of equimolar aqueous tetrabutylammonium hydroxide and was purified by semi-preparative reversed phase high performance liquid chromatography (c-18 hplc). the average radiochemical yield was approximately 21% end-of-synthesis (eos) with an average specific activity of over 2750 mci/mumol eos. the total time for synthesis and ...20103019944
an improved synthesis of (3-n-[11c]methyl)spiperone.an improved radiochemical synthesis of (3-n-[11c]methyl) spiperone is reported. the radiotracer was prepared by n-alkylation of spiperone in dimethylformamide in the presence of equimolar aqueous tetrabutylammonium hydroxide and was purified by semi-preparative reversed phase high performance liquid chromatography (c-18 hplc). the average radiochemical yield was approximately 21% end-of-synthesis (eos) with an average specific activity of over 2750 mci/mumol eos. the total time for synthesis and ...20103019944
inhibition and reversal of endotoxin-, aggregated igg- and paf-induced hypotension in the rat by sri 63-072, a paf receptor antagonist.platelet activating factor (paf) given intravenously produces systemic hypotension in the rat. similar effects can be induced using endotoxin or heat-aggregated igg challenges, which are thought to involve endogenous paf release. extending this concept, we have examined the ability of the paf antagonist sri 63-072 to inhibit or reverse systemic hypotension induced with paf, heat-aggregated igg or endotoxin 0111-b4 in rats. at 100 ng kg-1 paf, there occurred a 38.6 +/- 5.1% decrease in carotid me ...19863019921
inhibition and reversal of endotoxin-, aggregated igg- and paf-induced hypotension in the rat by sri 63-072, a paf receptor antagonist.platelet activating factor (paf) given intravenously produces systemic hypotension in the rat. similar effects can be induced using endotoxin or heat-aggregated igg challenges, which are thought to involve endogenous paf release. extending this concept, we have examined the ability of the paf antagonist sri 63-072 to inhibit or reverse systemic hypotension induced with paf, heat-aggregated igg or endotoxin 0111-b4 in rats. at 100 ng kg-1 paf, there occurred a 38.6 +/- 5.1% decrease in carotid me ...19863019921
evaluation of silica gel cartridges coated in situ with acidified 2,4-dinitrophenylhydrazine for sampling aldehydes and ketones in air.a procedure for coating in situ silica gel in prepacked cartridges with 2,4-dinitrophenylhydrazine (dnph) acidified with hydrochloric acid is described. the coated cartridge was compared with a validated dnph impinger method for sampling organic carbonyl compounds (aldehydes and ketones) in diluted automotive exhaust emissions and in ambient air for subsequent analysis of the dnph derivatives by high performance liquid chromatography. qualitative and quantitative data are presented that show tha ...20103019907
evaluation of silica gel cartridges coated in situ with acidified 2,4-dinitrophenylhydrazine for sampling aldehydes and ketones in air.a procedure for coating in situ silica gel in prepacked cartridges with 2,4-dinitrophenylhydrazine (dnph) acidified with hydrochloric acid is described. the coated cartridge was compared with a validated dnph impinger method for sampling organic carbonyl compounds (aldehydes and ketones) in diluted automotive exhaust emissions and in ambient air for subsequent analysis of the dnph derivatives by high performance liquid chromatography. qualitative and quantitative data are presented that show tha ...20103019907
pagetoid bowen's disease on the breast. 20103019906
pagetoid bowen's disease on the breast. 20103019906
phosphoglycolate phosphatase polymorphism: gene frequencies in three italian samples.the electrophoretic polymorphism of the pgp locus has been studied in about 1,700 italians. the sample consisted of individuals from viareggio (north-central italy), rome (central italy) and cagliari (sardinia, southern italy). comparison among the three groups showed a high degree of heterogeneity. the sardinian sample was well differentiated from the other two concerning the frequencies of both the pgp3 and of pgp2 alleles. the frequency of the pgp1 allele varied from 0.900 (viareggio) to 0.98 ...20103019873
phosphoglycolate phosphatase polymorphism: gene frequencies in three italian samples.the electrophoretic polymorphism of the pgp locus has been studied in about 1,700 italians. the sample consisted of individuals from viareggio (north-central italy), rome (central italy) and cagliari (sardinia, southern italy). comparison among the three groups showed a high degree of heterogeneity. the sardinian sample was well differentiated from the other two concerning the frequencies of both the pgp3 and of pgp2 alleles. the frequency of the pgp1 allele varied from 0.900 (viareggio) to 0.98 ...20103019873
up-regulation of brain [3h]diazepam binding sites in chronic caffeine treated rats.brain [3h]diazepam and [3h]l-phenylisopropyladenosine binding sites in caffeine treated (75 mg/kg/day, i.p. 12 days) and caffeine withdrawn (30 days) rats were examined. treatment with caffeine (75 mg/kg/day) for 12 days increases the bmax (maximum binding capacity) for [3h]diazepam binding by 30.9% whereas the same treatment increases the bmax for [3h]l-pia binding by 165%. the bmax for [3h]diazepam binding sites returns to slightly below control levels but [3h]l-pia binding sites remain elevat ...19863019827
up-regulation of brain [3h]diazepam binding sites in chronic caffeine treated rats.brain [3h]diazepam and [3h]l-phenylisopropyladenosine binding sites in caffeine treated (75 mg/kg/day, i.p. 12 days) and caffeine withdrawn (30 days) rats were examined. treatment with caffeine (75 mg/kg/day) for 12 days increases the bmax (maximum binding capacity) for [3h]diazepam binding by 30.9% whereas the same treatment increases the bmax for [3h]l-pia binding by 165%. the bmax for [3h]diazepam binding sites returns to slightly below control levels but [3h]l-pia binding sites remain elevat ...19863019827
enhanced relaxation response of canine coronary artery to isoproterenol and salbutamol after removal of endothelial cells.the importance of the endothelium for isoproterenol- and salbutamol-induced relaxation of canine coronary artery was examined. the relaxation effect of isoproterenol and salbutamol was significantly enhanced (p less than 0.05) upon removal of the coronary artery endothelium. in contrast, the relaxation effect of acetylcholine was completely abolished. these results indicate that canine coronary endothelium modulates beta-adrenoceptor-mediated relaxation, and that the role of the endothelium in a ...19863019826
enhanced relaxation response of canine coronary artery to isoproterenol and salbutamol after removal of endothelial cells.the importance of the endothelium for isoproterenol- and salbutamol-induced relaxation of canine coronary artery was examined. the relaxation effect of isoproterenol and salbutamol was significantly enhanced (p less than 0.05) upon removal of the coronary artery endothelium. in contrast, the relaxation effect of acetylcholine was completely abolished. these results indicate that canine coronary endothelium modulates beta-adrenoceptor-mediated relaxation, and that the role of the endothelium in a ...19863019826
modulation of receptor mediated leukotriene release in the perfused heart.the chemotactic peptide n-formyl-methionyl-leucyl-phenylalanine (fmlp) induces peptide leukotriene release at concentrations of 20-25 pmol/ml 3 min after the start of fmlp infusion. fmlp-induced leukotriene release in rabbit hearts is not blocked by the leukotriene receptor antagonist fpl-55712 at concentrations that totally antagonize the hemodynamic effects of exogenously infused peptide leukotrienes. moreover, propyl gallate, a lipoxygenase inhibitor, does not block fmlp-induced leukotriene r ...19863019823
modulation of receptor mediated leukotriene release in the perfused heart.the chemotactic peptide n-formyl-methionyl-leucyl-phenylalanine (fmlp) induces peptide leukotriene release at concentrations of 20-25 pmol/ml 3 min after the start of fmlp infusion. fmlp-induced leukotriene release in rabbit hearts is not blocked by the leukotriene receptor antagonist fpl-55712 at concentrations that totally antagonize the hemodynamic effects of exogenously infused peptide leukotrienes. moreover, propyl gallate, a lipoxygenase inhibitor, does not block fmlp-induced leukotriene r ...19863019823
the effects of hemicholinium-3 (hc-3) during tetanic (tp) and posttetanic potentiation (ptp) on sympathetic ganglion.an electrophysiological analysis has been made of the synaptic plasticity in the eighth sympathetic ganglion of the toad. the mean amplitude of compound action potential (cap) recorded extracellularly was taken as a measure of the synaptic transmission. repetitive stimulation of the preganglionic axonal fibers at 50 hz for 40 seconds in the presence of hc-3 led to facilitatory and depressant actions on ganglionic synaptic transmission. hc-3 in the presence of elevated extracellular ca2+ concentr ...20103019820
the effects of hemicholinium-3 (hc-3) during tetanic (tp) and posttetanic potentiation (ptp) on sympathetic ganglion.an electrophysiological analysis has been made of the synaptic plasticity in the eighth sympathetic ganglion of the toad. the mean amplitude of compound action potential (cap) recorded extracellularly was taken as a measure of the synaptic transmission. repetitive stimulation of the preganglionic axonal fibers at 50 hz for 40 seconds in the presence of hc-3 led to facilitatory and depressant actions on ganglionic synaptic transmission. hc-3 in the presence of elevated extracellular ca2+ concentr ...20103019820
effect of tropomyosin on the interactions of actin with actin-binding proteins isolated from pig platelets.several non-muscle tropomyosins have been reported to lack the ability to polymerize in a head-to-tail manner [dabrowska, r. et al. (1983) j. muscle res. cell motil. 1, 83-92; côté, g.p. (1983) mol. cell. biochem. 57, 127-146]. unlike rabbit skeletal muscle tropomyosin, these proteins could therefore not protect the f-actin microfilaments neither from disassembly or from cross-linking by the other actin-associating factors. however, we have provided evidence that, in vitro, pig platelet tropomyo ...19863019696
effect of tropomyosin on the interactions of actin with actin-binding proteins isolated from pig platelets.several non-muscle tropomyosins have been reported to lack the ability to polymerize in a head-to-tail manner [dabrowska, r. et al. (1983) j. muscle res. cell motil. 1, 83-92; côté, g.p. (1983) mol. cell. biochem. 57, 127-146]. unlike rabbit skeletal muscle tropomyosin, these proteins could therefore not protect the f-actin microfilaments neither from disassembly or from cross-linking by the other actin-associating factors. however, we have provided evidence that, in vitro, pig platelet tropomyo ...19863019696
the budding mechanism of spikeless vesicular stomatitis virus particles.virus particles, lacking the spike g-glycoproteins, are produced during infection of vero cells with the vesicular stomatitis virus mutant ts045 at the restrictive temperature 39.5 degrees c. at this temperature the mutated g proteins are blocked in their intracellular transport in the endoplasmic reticulum. we have studied the role of the g proteins in the formation of these spikeless virus particles. the results showed that the spikeless particles contain a full complement of membrane anchors, ...19863019669
the budding mechanism of spikeless vesicular stomatitis virus particles.virus particles, lacking the spike g-glycoproteins, are produced during infection of vero cells with the vesicular stomatitis virus mutant ts045 at the restrictive temperature 39.5 degrees c. at this temperature the mutated g proteins are blocked in their intracellular transport in the endoplasmic reticulum. we have studied the role of the g proteins in the formation of these spikeless virus particles. the results showed that the spikeless particles contain a full complement of membrane anchors, ...19863019669
characterization of in vitro dopamine synthesis in the median eminence of rats with haloperidol-induced hyperprolactinemia and bromocriptine-induced hypoprolactinemia.in order to investigate the mechanism of prl action on dopamine synthesis in tuberoinfundibular dopaminergic (tida) neurons, in vitro dopamine synthesis in the median eminence of hypothalamic slices was compared between hyperprolactinemic and hypoprolactinemic rats, hyper- and hypoprolactinemia were induced in ovariectomized rats by repetitive injections of the dopamine antagonist haloperidol (halo) and the dopamine agonist bromocriptine (bromo), respectively. in vitro dopamine synthesis in tida ...19863019639
nuclear thyroid hormone receptors in human cancer tissues.saturable, high affinity binding sites for 3,5,3' triiodothyronine (t3) were identified in nuclei isolated from human tumors of various origins (breast cancers, other epitheliomas, sarcomas, tumors of the central nervous system). nuclear t3 receptors were present in all samples of primary breast cancer (n = 93; average cmax = 215 fmol/mg dna) and in metastatic tissues originating from breast tumors. a significantly lower t3 binding capacity was found in non-tumor tissues, obtained from breast si ...19863019222
cortisol and corticosterone response after syn-corticotropin in relationship to dexamethasone suppressibility of cortisol.the current study was designed to investigate whether glucocorticoid output after syn-acth stimulation is different in depression associated with dexamethasone suppression test (dst) nonsuppression from the euthymic state and dst suppression. we gave 28 depressives a dst and an adrenocortical challenge with synthetic acth. fourteen patients were nonsuppressors on the dst. after successful drug treatment, the subjects were reinvestigated by both tests; all dsts revealed plasma cortisol concentrat ...20103018821
cortisol and corticosterone response after syn-corticotropin in relationship to dexamethasone suppressibility of cortisol.the current study was designed to investigate whether glucocorticoid output after syn-acth stimulation is different in depression associated with dexamethasone suppression test (dst) nonsuppression from the euthymic state and dst suppression. we gave 28 depressives a dst and an adrenocortical challenge with synthetic acth. fourteen patients were nonsuppressors on the dst. after successful drug treatment, the subjects were reinvestigated by both tests; all dsts revealed plasma cortisol concentrat ...20103018821
developmental regulation of transcript splicing in drosophila melanogaster. 20093018778
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