Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| monitoring of fungal loads in seabird rehabilitation centers with comparisons to natural seabird environments in northern california. | aspergillosis remains a major cause of mortality in captive and rehabilitated seabirds. to date, there has been poor documentation of fungal (particularly aspergillus spp.) burdens in natural seabird loafing and roosting sites compared with fungal numbers in rehabilitation or captive settings and the various microenvironments that seabirds are exposed to during the rehabilitation process. this study compares fungal, particularly aspergillus spp., burdens potentially encountered by seabirds in na ... | 2014 | 24712159 |
| isolated aspergillosis myocardial abscesses in a liver-transplant patient. | cardiac abscess is an uncommon and fatal complication after transplantation. we report a case of an initially isolated aspergillosis myocardial abscess diagnosed by cardiac magnetic resonance imaging (cmri). at that time, there was no other biological evidence or other extracardiac manifestations. a three-month course of dual antifungal therapy followed by a single antifungal therapy was empirically given. six month after admission, aspergillus fumigatus was isolated for the first time and the p ... | 2014 | 24707433 |
| induction of gliotoxin secretion in aspergillus fumigatus by bacteria-associated molecules. | aspergillus fumigatus is the most common causative agent of mold diseases in humans, giving rise to life-threatening infections in immunocompromised individuals. one of its secreted metabolites is gliotoxin, a toxic antimicrobial agent. the aim of this study was to determine whether the presence of pathogen-associated molecular patterns in broth cultures of a. fumigatus could induce gliotoxin production. gliotoxin levels were analyzed by ultra-performance liquid chromatography and mass spectrome ... | 2014 | 24705440 |
| aspergillus endocarditis in a paediatric patient after a cardiac surgery, associated with septic pulmonary embolism and pulmonary hypertension. | we report a rare case of pulmonary prosthetic valve endocarditis due to aspergillus fumigatus, associated with septic pulmonary embolism and secondary pulmonary hypertension, in a 4-year-old boy with surgically corrected tetralogy of fallot. the diagnosis and treatment of aspergillus endocarditis remains highly challenging. the best therapeutic option for chronic thromboembolic pulmonary hypertension due to an infectious thromboembolic event is highly debatable and the results are poor. | 2015 | 24702799 |
| invasive aspergillosis in kidney transplant recipients: a cohort study. | fungal infections represent 5% of infections in renal transplant recipients. candidiasis is the most frequent, followed by aspergillosis and cryptococcosis. invasive aspergillosis is severe and has a poor prognosis, but recent series focused on outcomes are scarce in the literature. we analyzed invasive aspergillosis cases in a cohort of kidney transplant recipients and suggest that prognosis is improved with early diagnosis and treatment. | 2014 | 24702140 |
| crystallographic characterization of the (r)-selective amine transaminase from aspergillus fumigatus. | the importance of amine transaminases for producing optically pure chiral precursors for pharmaceuticals and chemicals has substantially increased in recent years. the x-ray crystal structure of the (r)-selective amine transaminase from the fungus aspergillus fumigatus was solved by s-sad phasing to 1.84 å resolution. the refined structure at 1.27 å resolution provides detailed knowledge about the molecular basis of substrate recognition and conversion to facilitate protein-engineering approache ... | 2014 | 24699652 |
| increased prevalence and altered species composition of filamentous fungi in respiratory specimens from cystic fibrosis patients. | filamentous fungi cultured from respiratory tract specimens submitted to the department of clinical microbiology, aarhus university hospital, during 2010 were identified by morphology and by internal transcribed spacer (its) sequencing. of 343 fungal isolates, discrepancies between identification methods were observed for four isolates (1.2%), while identification to species was achieved only with its sequencing for 16 isolates (4.7%). filamentous fungi were isolated from 15% of cystic fibrosis ... | 2014 | 24698301 |
| azole-resistant aspergillus fumigatus in the environment of northern italy, may 2011 to june 2012. | in recent years acquired azole resistance in aspergillus fumigatus has been increasingly reported and a dominant mechanism of resistance (tr34/l98h) was found in clinical and environmental isolates. the aim of the present study was to investigate the prevalence of azole resistance in environmental a. fumigatus isolates collected in northern italy. a. fumigatus grew from 29 of 47 soil samples analysed. azole-resistant isolates were detected in 13% (6/47) of the soil samples and in 21% (6/29) of t ... | 2014 | 24698139 |
| structure and function of tmem16 proteins (anoctamins). | tmem16 proteins, also known as anoctamins, are involved in a variety of functions that include ion transport, phospholipid scrambling, and regulation of other membrane proteins. the first two members of the family, tmem16a (anoctamin-1, ano1) and tmem16b (anoctamin-2, ano2), function as ca2+-activated cl- channels (caccs), a type of ion channel that plays important functions such as transepithelial ion transport, smooth muscle contraction, olfaction, phototransduction, nociception, and control o ... | 2014 | 24692353 |
| a novel antifungal protein with lysozyme-like activity from seeds of clitoria ternatea. | an antifungal protein with a molecular mass of 14.3 kda was isolated from the seeds of butterfly pea (clitoria ternatea) and designated as ct protein. the antifungal protein was purified using different methods including ammonium sulphate precipitation, ion exchange chromatography on deae-cellulose and gel filtration on sephadex g-50 column. ct protein formed a single colourless rod-shaped crystal by hanging drop method after 7 days of sample loading. the protein showed lytic activity against mi ... | 2014 | 24691882 |
| development and validation of an hplc-ms/ms method for the early diagnosis of aspergillosis. | invasive aspergillosis is an opportunistic infection that is mainly caused by aspergillus fumigatus, which is known to produce several secondary metabolites, including gliotoxin, the most abundant metabolite produced during hyphal growth. the diagnosis of invasive aspergillosis is often made late in the infection because of the lack of reliable and feasible diagnostic techniques; therefore, early detection is critical to begin treatment and avoid more serious complications. the present work repo ... | 2014 | 24690884 |
| occupational hypersensitivity pneumonitis in a baker: a new cause. | bakers are exposed daily to flour and may be susceptible to immunologic occupational diseases. a 30-year-old, nonsmoking, female baker was referred for progressive dyspnea on exertion, basal crackles on auscultation, restrictive lung function, decreased diffusing capacity of the lung for carbon monoxide, ground glass hyperdensities with a mosaic pattern on high-resolution ct scan, 25% lymphocytosis by bal, and cellular chronic bronchiolitis with peribronchiolar interstitial inflammation by lung ... | 2014 | 24687706 |
| host biomarkers of invasive pulmonary aspergillosis to monitor therapeutic response. | invasive pulmonary aspergillosis (ipa) is a life-threatening disease of immunocompromised patients that requires aggressive therapy. detection of the disease and monitoring of the therapeutic response during ipa are complex, and current molecular diagnostics are not suitably robust. here, we explored proteomic profiles of bronchoalveolar lavage fluid (balf) specimens from a persistently neutropenic rabbit model of ipa. three experimental arms, uninfected control animals, infected untreated anima ... | 2014 | 24687510 |
| aspergillus tracheobronchitis in critically ill patients with chronic obstructive pulmonary diseases. | aspergillus tracheobronchitis (atb) is considered as an unusual form of invasive aspergillosis and has a fatal outcome. there is little current information on several aspects of chronic obstructive pulmonary diseases (copd) complicated by atb, the frequency of which is expected to increase in the coming years. in a prospective study of invasive bronchial-pulmonary aspergillosis (ibpa) in a critically ill copd population, three proven cases of atb were identified. the three new cases, combined wi ... | 2014 | 24673772 |
| loss of regulator of g protein signaling 5 promotes airway hyperresponsiveness in the absence of allergic inflammation. | although eosinophilic inflammation typifies allergic asthma, it is not a prerequisite for airway hyperresponsiveness (ahr), suggesting that underlying abnormalities in structural cells, such as airway smooth muscle (asm), contribute to the asthmatic diathesis. dysregulation of procontractile g protein-coupled receptor (gpcr) signaling in asm could mediate enhanced contractility. | 2014 | 24666695 |
| synthesis and biological evaluation of 2-aminobenzamide derivatives as antimicrobial agents: opening/closing pharmacophore site. | a series of new 2-aminobenzamide derivatives (1-10) has been synthesized in good to excellent yields by adopting both conventional and/or a time-efficient microwave assisted methodologies starting from isatoic anhydride (isa) and characterized on the basis of their physical, spectral and microanalytical data. selected compounds of this series were then tested against various bacterial (bacillus subtilis (rcmb 000107) and staphylococcus aureus (rcmb 000106). pseudomonas aeruginosa (rcmb 000102) a ... | 2014 | 24663060 |
| axl receptor blockade ameliorates pulmonary pathology resulting from primary viral infection and viral exacerbation of asthma. | viruses use tyro3, axl, and mertk (tam) receptor tyrosine kinases to infect and modulate the immune properties of various cell types, which led us to investigate whether tam receptor activation affected primary viral infection and viral exacerbation of asthma in experimental models. in these lung-specific models, we observed that axl was the most abundantly induced tam receptor protein. during primary respiratory syncytial virus (rsv) infection, anti-axl mab treatment significantly increased the ... | 2014 | 24659691 |
| (1)h, (13)c and (15)n resonance assignments of the roda hydrophobin from the opportunistic pathogen aspergillus fumigatus. | hydrophobins are fungal proteins characterised by their amphipathic properties and an idiosyncratic pattern of eight cysteine residues involved in four disulphide bridges. the soluble form of these proteins spontaneously self-assembles at hydrophobic/hydrophilic interfaces to form an amphipathic monolayer. the roda hydrophobin of the opportunistic pathogen aspergillus fumigatus forms an amyloid layer with a rodlet morphology that covers the surface of fungal spores. this rodlet layer bestows hyd ... | 2015 | 24659460 |
| a 7-dimethylallyl tryptophan synthase from a fungal neosartorya sp.: biochemical characterization and structural insight into the regioselective prenylation. | a putative 7-dimethylallyl tryptophan synthase (dmats) gene from a fungal neosartorya sp. was cloned and overexpressed as a soluble his6-fusion protein in escherichia coli. the enzyme was found to catalyze the prenylation of l-tryptophan at the c7 position of the indole moiety in the presence of dimethylallyl diphosphate; thus, it functions as a 7-dmats. in this study, we describe the biochemical characterization of 7-dmats from neosartorya sp., referred to as 7-dmats(neo), and the structural ba ... | 2014 | 24657051 |
| aspergillus monitoring project in a large educational hospital using molecular assay. | it is important to find reliable and accessible methods for the diagnosis and identification of fungal species causing hospital acquired infections. our main objective was using a rapid and accessible molecular method for the monitoring of aspergillus infections and identification of causing agents in the level of species. | 2014 | 24653809 |
| fermented whey as poultry feed additive to prevent fungal contamination. | fungal contamination of poultry feed causes economic losses to industry and represents a potential risk to animal health. the aim of the present study was to analyze the effectiveness of whey fermented with kefir grains as additive to reduce fungal incidence, thus improving feed safety. | 2014 | 24652751 |
| steam-treatment-based soil remediation promotes heat-tolerant, potentially pathogenic microbiota. | we investigated microbiota in surface and subsurface soil from a site, above steam-treated deep sub-soil originally contaminated with chlorinated solvents. during the steam treatment, the surface soil reached temperatures c. 30 degrees c higher than the temperature in untreated soil; whereas the subsurface soil, at a depth of about 40 cm, reached a temperature c. 45 degrees c higher than untreated soil. the soil was examined prior to, during, and 6, 12, 14, 20 and 31 months after treatment. numb ... | 2014 | 24645459 |
| gliotoxin promotes aspergillus fumigatus internalization into type ii human pneumocyte a549 cells by inducing host phospholipase d activation. | the internalization of aspergillus fumigatus into lung epithelial cells is critical for the infection process in the host. gliotoxin is the most potent toxin produced by a. fumigatus. however, its role in a. fumigatus internalization into the lung epithelial cells is still largely unknown. in the present study, the deletion of the glip gene regulating the production of gliotoxin in a. fumigatus suppressed the internalization of conidia into the a549 lung epithelial cells, and this suppression co ... | 2014 | 24637030 |
| expression of dectin-1 during fungus infection in human corneal epithelial cells. | to evaluate the expression of dendritic cell-associated c-type lectin-1 (dectin-1) in human corneal epithelial (hce) cells infected by fungus. | 2014 | 24634860 |
| enhanced production of fumigaclavine c by ultrasound stimulation in a two-stage culture of aspergillus fumigatus cy018. | stimulation by physical means including ultrasound is important to cell morphology and the product yield. in this work, the effect of ultrasound on the production of fumigaclavine c (fc), a conidiation-associated alkaloid with strong anti-inflammatory activity, was investigated in a newly developed two-stage culture of aspergillus fumigatus cy018. the optimum ultrasonication conditions consisted of exposing cultures (at 12h of growth phase) to 10-min repeated irradiation (4 times) with a 24-h in ... | 2014 | 24632633 |
| design, synthesis & evaluation of condensed 2h-4-arylaminopyrimidines as novel antifungal agents. | a small, focussed library of condensed 2h-4-arylaminopyrimidines, with 3-diversity points, based on an initial design by molecular docking study of this scaffold at the active site of the fungal enzyme of cytochrome p450 family, lanosterol 14α-demethylase (cyp51) was synthesized through a one-pot green chemical synthetic protocol. the screening of the synthesised compounds for antifungal activity against candida albicans, aspergillus fumigatus &aspergillus niger revealed activity in many of the ... | 2014 | 24631896 |
| self-assembly of proteins into a three-dimensional multilayer system: investigation of the surface of the human fungal pathogen aspergillus fumigatus. | hydrophobins are small surface active proteins that fulfil a wide spectrum of functions in fungal growth and development. the human fungal pathogen aspergillus fumigatus expresses roda hydrophobins that self-assemble on the outer conidial surface into tightly organized nanorods known as rodlets. afm investigation of the conidial surface allows us to evidence that roda hydrophobins self-assemble into rodlets through bilayers. within bilayers, hydrophilic domains of hydrophobins point inward, thus ... | 2014 | 24631542 |
| role of inhaled amphotericin in allergic bronchopulmonary aspergillosis. | allergic bronchopulmonary aspergillosis (abpa) is an immunological pulmonary disorder caused by immune reactions mounted against the ubiquitous fungus aspergillus fumigatus. the disease clinically manifests with poorly controlled asthma, hemoptysis, systemic manifestations like fever, anorexia and weight loss, fleeting pulmonary opacities and bronchiectasis. the natural course of the disease is characterized by repeated episodes of exacerbations. almost 30-40% of the patients require prolonged t ... | 2015 | 24625938 |
| protection against experimental aspergillosis by heat-killed yeast is not antibody dependent. | previously we showed heat-killed yeast (hky) of saccharomyces cerevisiae administered as a vaccine are protective against systemic murine aspergillosis (and other mycoses) and that hky induces antibody and cellular responses. to determine the role of antibodies in this protection, male antibody knockout mice (ko; strain b6.129s2-igh-6 (tm1cgn)/j) and c57bl/6 wild-type (wt) mice were vaccinated subcutaneously with 6 × 10(7) hky or phosphate buffered saline (pbs) given three or four times. mice we ... | 2014 | 24625671 |
| fungi in the cystic fibrosis lung: bystanders or pathogens? | improvement to the life expectancy of people with cystic fibrosis (pwcf) brings about novel challenges including the need for evaluation of the role of fungi in the cystic fibrosis (cf) lung. to determine if such organisms represent bystanders or pathogens affecting clinical outcomes we review the existing knowledge from a clinical, biochemical, inflammatory and immunological perspective. the prevalence and importance of fungi in the cf airway has likely been underestimated with the most frequen ... | 2014 | 24625547 |
| fumigatosides a-d, four new glucosidated pyrazinoquinazoline indole alkaloids from a jellyfish-derived fungus aspergillus fumigatus. | 2014 | 24625282 | |
| invasive pulmonary aspergillosis post extracorporeal membrane oxygenation support and literature review. | the use of extracorporeal membrane oxygenation (ecmo) for reversible pulmonary failure in critically ill patients has increased over the last few decades. nosocomial infections are a major complication of ecmo and fungi have been found to be a common cause. herein, we describe a case of invasive pulmonary aspergillosis following ecmo, which was successfully treated with combination antifungal therapy and interferon-gamma. | 2014 | 24624325 |
| increased production of gliotoxin is related to the formation of biofilm by aspergillus fumigatus: an immunological approach. | gliotoxin (gt) belongs to the epipolythiodioxopiperazine class of toxins secreted from certain fungi including aspergillus fumigatus, which is the most prolific producer of this secondary metabolite. recently, enhanced amounts of gt were found in in vitro biofilm-grown a. fumigatus mycelium. to further correlate the a. fumigatus biofilm growth phenotype with the enhanced secretion of gt, a polyclonal antibody (pab) was produced by immunizing mice against gt. by an indirect immunofluorescent assa ... | 2014 | 24623580 |
| evaluation of invasive aspergillosis risk of immunocompromised patients alternatively hospitalized in hematology intensive care unit and at home. | contrary to hospital exposure, little is known about the indoor fungal exposure of hematology patients at home. the aim of our study was to investigate the mold exposure of hematology patients both at home and at hospital to assess their invasive aspergillosis (ia) risk. fungal exposure was assessed by quantifying opportunistic molds at hospital during hospitalization and in homes of 53 hematology patients. ia was diagnosed in 13 of 53 patients and invasive fungal infection (ifi) in one patient. ... | 2014 | 24621176 |
| fumagillin: an overview of recent scientific advances and their significance for apiculture. | fumagillin is a potent fungal metabolite first isolated from aspergillus fumigatus. it is widely used in apiculture and human medicine against a variety of microsporidian fungal infections. it has been the subject of research in cancer treatments by employing its angiogenesis inhibitory properties. the toxicity of fumagillin has limited its use for human applications and spurred the development of analogues using structure-activity relationships relating to its angiogenesis properties. these dis ... | 2014 | 24621007 |
| microsecond pulsed magnetic field improves efficacy of antifungal agents on pathogenic microorganisms. | control and treatment of the emerging filamentous and yeast fungal diseases are of high priority in the biomedical field. this study investigated the influence of the pulsed magnetic field combined with common antifungal agents on the viability of various pathogenic fungi such as aspergillus fumigatus, candida albicans, and trychophyton rubrum. repetitive microsecond pulsed magnetic fields up to 6.1 t were applied in the study. the synergistic effect of co-applying drugs and magnetic treatment t ... | 2014 | 24619828 |
| management of recurrent postoperative fungal endophthalmitis. | to report the management of recurrent postoperative fungal endophthalmitis (pofe) after failed pars plana vitrectomy (ppv) and antifungal therapy. | 2014 | 24618484 |
| the protein phosphatase phza of a. fumigatus is involved in oxidative stress tolerance and fungal virulence. | protein phosphatases z that are unique to the fungal kingdom have been associated to resistance to high salt concentration, cell wall integrity, cell cycle regulation, and oxidative stress in fungi. in aspergillus fumigatus, it was shown that phza is under the control of the transcription factor skn7 and is only involved in the control of the oxidative stress. accordingly, the δphza mutant showed a defect in virulence in an experimental model of corneal infection in immunocompetent animals and t ... | 2014 | 24614084 |
| co-ordination between brla regulation and secretion of the oxidoreductase fmqd directs selective accumulation of fumiquinazoline c to conidial tissues in aspergillus fumigatus. | aerial spores, crucial for propagation and dispersal of the kingdom fungi, are commonly the initial inoculum of pathogenic fungi. natural products (secondary metabolites) have been correlated with fungal spore development and enhanced virulence in the human pathogen aspergillus fumigatus but mechanisms for metabolite deposition in the spore are unknown. metabolomic profiling of a. fumigatus deletion mutants of fumiquinazoline (fq) cluster genes reveal that the first two products of the fq cluste ... | 2014 | 24612080 |
| structure-activity relationships among antifungal nylon-3 polymers: identification of materials active against drug-resistant strains of candida albicans. | fungal infections are a major challenge to human health that is heightened by pathogen resistance to current therapeutic agents. previously, we were inspired by host-defense peptides to develop nylon-3 polymers (poly-β-peptides) that are toxic toward the fungal pathogen candida albicans but exert little effect on mammalian cells. based on subsequent analysis of structure-activity relationships among antifungal nylon-3 polymers, we have now identified readily prepared cationic homopolymers active ... | 2014 | 24606327 |
| a polysaccharide virulence factor from aspergillus fumigatus elicits anti-inflammatory effects through induction of interleukin-1 receptor antagonist. | the galactosaminogalactan (gag) is a cell wall component of aspergillus fumigatus that has potent anti-inflammatory effects in mice. however, the mechanisms responsible for the anti-inflammatory property of gag remain to be elucidated. in the present study we used in vitro pbmc stimulation assays to demonstrate, that gag inhibits proinflammatory t-helper (th)1 and th17 cytokine production in human pbmcs by inducing interleukin-1 receptor antagonist (il-1ra), a potent anti-inflammatory cytokine t ... | 2014 | 24603878 |
| in vitro effects of candida albicans and aspergillus fumigatus on dendritic cells and the role of beta glucan in this effect. | dendritic cells (dcs) are able to initiate and regulate the immune response to fungal infections. β-glucan stimulates the immune system, modulating cellular and humoral immunity. it has a beneficial effect in fighting fungal infections. | 2014 | 24595999 |
| design and synthesis of novel 2h-chromen-2-one derivatives bearing 1,2,3-triazole moiety as lead antimicrobials. | a series of novel 2h-chromen-2-one derivatives decorated with 1,2,3-triazole moiety were designed and synthesized using the click reaction of azidoalkyloxy-2h-chromen-2-ones with different propargylamines. propargylamines were obtained by alkylation of various heterocyclic amines with propargyl bromide. newly synthesized compounds and intermediates were evaluated for their antifungal activity against four fungi (aspergillus niger, aspergillus fumigatus, aspergillus flavus and candida albicans). ... | 2014 | 24594353 |
| antifungal treatments delineate a correlation between cathepsins and cytokines in murine model of invasive aspergillosis. | in the pathogenesis of invasive pulmonary aspergillosis both fungal and host factors play roles. though cytokines and phagocyte, as host factors, have been shown to participate in defence against aspergillus species yet the role of cysteine proteases, that is cathepsins, a lysosomal enzymes of phagocytes, remains unknown in fungal infection. studies are available which shows that cytokines regulate the cysteine proteases processed immune molecules for their further action but their relationship ... | 2013 | 24591744 |
| development and antimicrobial susceptibility studies of in vitro monomicrobial and polymicrobial biofilm models with aspergillus fumigatus and pseudomonas aeruginosa. | mixed microbial infections of the respiratory tracts with p. aeruginosa and a. fumigatus capable of producing biofilms are commonly found in cystic fibrosis patients. the primary objective of this study was to develop an in vitro model for p. aeruginosa and a. fumigatus polymicrobial biofilm to study the efficacy of various antimicrobial drugs alone and in combinations against biofilm-embedded cells. simultaneous static cocultures of p. aeruginosa and sporelings were used for the development of ... | 2014 | 24588809 |
| inflammatory monocytes orchestrate innate antifungal immunity in the lung. | aspergillus fumigatus is an environmental fungus that causes invasive aspergillosis (ia) in immunocompromised patients. although -cc-chemokine receptor-2 (ccr2) and ly6c-expressing inflammatory monocytes (ccr2⁺mo) and their derivatives initiate adaptive pulmonary immune responses, their role in coordinating innate immune responses in the lung remain poorly defined. using conditional and antibody-mediated cell ablation strategies, we found that ccr2⁺mo and monocyte-derived dendritic cells (mo-dcs ... | 2014 | 24586155 |
| glionitrin a, a new diketopiperazine disulfide, activates atm-atr-chk1/2 via 53bp1 phosphorylation in du145 cells and shows antitumor effect in xenograft model. | in a recent study, we isolated the diketopiperazine disulfide glionitrin a from the co-culture broth of a mine drainage-derived fungus (aspergillus fumigatus kmc901) and bacterium (sphingomonas kmk001). here, we investigated the antitumor activity of glionitrin a and its underlying molecular mechanisms in human prostate cancer du145 cells. glionitrin a showed significant cytotoxicity, promoting cell cycle arrest and apoptosis. glionitrin a-treated cells exhibited elevated levels of phospho-histo ... | 2014 | 24583858 |
| imidazolylchromanones containing non-benzylic oxime ethers: synthesis and molecular modeling study of new azole antifungals selective against cryptococcus gattii. | a series of imidazolylchromanone oximes containing phenoxyethyl ether moiety, as found in omoconazole, were synthesized and evaluated against yeasts (candida albicans and cryptococcus gattii) and filamentous fungi (aspergillus fumigatus and exophiala dermatitidis). although the title compounds showed marginal activity against filamentous fungi but all of them exhibited potent activity against c. gattii (mic values ≤4 μg/ml). among them, (3-chlorophenoxy)ethyl analog 7c with mic value of 0.5 μg/m ... | 2014 | 24583607 |
| an invertebrate model to evaluate virulence in aspergillus fumigatus: the role of azole resistance. | the impact of different mutations in the aspergillus fumigatus ergosterol biosynthesis pathway on pathogenesis has been evaluated using a simple invertebrate mini host, the caterpillar galleria mellonella. a set of strains that includes clinical isolates and isogenic mutants with mutations at the cyp51a gene conferring azole resistance were studied. all strains demonstrated a similar in vitro growth pattern and are equally virulent against the insect larvae. these results suggest that in a. fumi ... | 2014 | 24577012 |
| dual localization of mdj1 in pathogenic fungi varies with growth temperature. | paracoccidioides brasiliensis and p. lutzii are temperature-dependent dimorphic fungi that cause paracoccidioidomycosis (pcm). previously, we characterized the pbmdj1 gene. this gene encodes p. brasiliensis chaperone mdj1, which in yeast is a mitochondrial member of the j-domain family, whose main function is to regulate cognate hsp70 activities. we produced rabbit polyclonal antibody antirecombinant pbmdj1 (rpbmdj1), which labeled the protein not only in mitochondria but also at the cell wall o ... | 2014 | 24577000 |
| crude extract from ziziphus jujuba fruits, a weapon against pediatric infectious disease. | pediatric infectious disease is one of the main problems in cancerous children that treat by chemotherapy drugs. thus, study in this regard is necessary. the aim of this study was to evaluate antimicrobial properties of ethanolic extract of ziziphus jujuba fruits against different infectious pathogens. | 2013 | 24575267 |
| mutations in the cyp51a gene and susceptibility to itraconazole in aspergillus fumigatus isolated from avian farms in france and china. | azole resistance in the fungal pathogen aspergillus fumigatus is an emerging problem and may develop during azole therapy in humans and animals or exposure to azole fungicides in the environment. to assess the potential risk of azole-resistance emergence in avian farms where azole compounds are used for the control of avian mycoses, we conducted a drug susceptibility study including a. fumigatus isolates from birds and avian farms in france and southern china. a total number of 175 isolates were ... | 2014 | 24570417 |
| predicting essential genes for identifying potential drug targets in aspergillus fumigatus. | aspergillus fumigatus (af) is a ubiquitous and opportunistic pathogen capable of causing acute, invasive pulmonary disease in susceptible hosts. despite current therapeutic options, mortality associated with invasive af infections remains unacceptably high, increasing 357% since 1980. therefore, there is an urgent need for the development of novel therapeutic strategies, including more efficacious drugs acting on new targets. thus, as noted in a recent review, "the identification of essential ge ... | 2014 | 24569026 |
| polysome profiling reveals broad translatome remodeling during endoplasmic reticulum (er) stress in the pathogenic fungus aspergillus fumigatus. | the unfolded protein response (upr) is a network of intracellular signaling pathways that supports the ability of the secretory pathway to maintain a balance between the load of proteins entering the endoplasmic reticulum (er) and the protein folding capacity of the er lumen. current evidence indicates that several pathogenic fungi rely heavily on this pathway for virulence, but there is limited understanding of the mechanisms involved. the best known functional output of the upr is transcriptio ... | 2014 | 24568630 |
| generation of complexity in fungal terpene biosynthesis: discovery of a multifunctional cytochrome p450 in the fumagillin pathway. | fumagillin (1), a meroterpenoid from aspergillus fumigatus, is known for its antiangiogenic activity due to binding to human methionine aminopeptidase 2. 1 has a highly oxygenated structure containing a penta-substituted cyclohexane that is generated by oxidative cleavage of the bicyclic sesquiterpene β-trans-bergamotene. the chemical nature, order, and biochemical mechanism of all the oxygenative tailoring reactions has remained enigmatic despite the identification of the biosynthetic gene clus ... | 2014 | 24568283 |
| high weekly doses of liposomal amphotericin b as secondary prophylaxis after cerebral aspergillosis in a paediatric patient. | a paediatric patient treated for acute lymphoblastic leukaemia developed cerebral abscesses caused by aspergillus fumigatus. after surgical draining voriconazole treatment was started. the patient developed a steven-johnson syndrome and treatment was switched to l-amb. the patient developed no new fungal lesions and l-amb treatment was continued until the end of the therapy. complete remission was achieved without neurological consequences. high dose l-amb represents an alternative for secondary ... | 2014 | 24567890 |
| intrapulmonary posaconazole penetration at the infection site in an immunosuppressed murine model of invasive pulmonary aspergillosis receiving oral prophylactic regimens. | adequate penetration to the infection/colonization site is crucial to attain optimal efficacy of posaconazole against aspergillus fumigatus diseases. we evaluated posaconazole exposure in pulmonary epithelial lining fluid (elf) in a murine model of invasive pulmonary aspergillosis. the posaconazole exposure (area under the plasma concentration-time curve from time zero to 24 h postinfusion [auc0-24]) in elf was 20% to 31% of that in plasma for total drug after the third dose, and the relationshi ... | 2014 | 24566183 |
| exploring thermophilic cellulolytic enzyme production potential of aspergillus fumigatus by the solid-state fermentation of wheat straw. | cellulases can be used for biofuel production to decrease the fuel crises in the world. microorganisms cultured on lignocellulosic wastes can be used for the production of cellulolytic enzymes at large scale. in the current study, cellulolytic enzyme production potential of aspergillus fumigatus was explored and optimized by employing various cultural and nutritional parameters. maximum endoglucanase production was observed after 72 h at 55 °c, ph 5.5, and 70 % moisture level. addition of 0.3 % ... | 2014 | 24562980 |
| thermal and physical stresses induce a short-term immune priming effect in galleria mellonella larvae. | exposure of larvae of galleria mellonella larvae to mild physical (i.e. shaking) or thermal stress for 24h increased their ability to survive infection with aspergillus fumigatus conidia however larvae stressed in a similar manner but incubated for 72h prior to infection showed no elevation in their resistance to infection with a. fumigatus. stressed larvae demonstrated an elevated haemocyte density 24h after initiation of the stress event but this declined at 48 and 72h. larval proteins such as ... | 2014 | 24561359 |
| microorganisms associated particulate matter: a preliminary study. | this study aims to determine the microbiological quality of particulate matter (pm) in an urban area in jeddah, saudi arabia, during december 2012 to april 2013. this was achieved by the determination of airborne bacteria, fungi, and actinobacteria associated pm10 and pm2.5, as well as their relationships with gaseous pollutants, o3, so2 and no2, and meteorological factors (t°c, rh% and ws). high volume samplers with pm10 and pm2.5 selective sizes, and glass fiber filters were used to collect pm ... | 2014 | 24561289 |
| what causes canine sino-nasal aspergillosis? a molecular approach to species identification. | on the basis of phenotypic identification methods, aspergillus fumigatus is reported as the most commonly identified aetiological agent of canine sino-nasal aspergillosis (sna). however, definitive identification of aspergillus spp. using phenotypic features alone is unreliable. the aim of this study was to determine the molecular identities of fungal species causing sna in dogs. genomic dna was extracted from 91 fungal isolates from 90 dogs diagnosed with sna in australia, the usa and belgium, ... | 2014 | 24559745 |
| fungal contamination in two portuguese wastewater treatment plants. | the presence of filamentous fungi was detected in wastewater and air collected at wastewater treatment plants (wwtp) from several european countries. the aim of the present study was to assess fungal contamination in two wwtp operating in lisbon. in addition, particulate matter (pm) contamination data was analyzed. to apply conventional methods, air samples from the two plants were collected through impaction using an air sampler with a velocity air rate of 140 l/min. surfaces samples were colle ... | 2014 | 24555650 |
| azole-resistant aspergillus fumigatus isolate with the tr34/l98h mutation in both a fungicide-sprayed field and the lung of a hematopoietic stem cell transplant recipient with invasive aspergillosis. | a french farmer developed invasive aspergillosis with azole-resistant aspergillus fumigatus with the tr34/l98h mutation following a hematopoietic stem cell transplantation. he had worked in fungicide-sprayed fields where a non-genetically related a. fumigatus tr34/l98h isolate was collected. if azole resistance detection increases, voriconazole as first-line therapy might be questioned in agricultural areas. | 2014 | 24554754 |
| contaminants in human nail dust: an occupational hazard in podiatry? | there has been limited literature indicating that podiatrists' health may be at risk from exposure to human nail dust. previous studies carried out in the uk have shown that large amounts of dust become airborne during the human nail drilling procedure and are present in the air up to 10 hours after a clinical session. this increases the risk of respiratory tract (rt) infection for the practitioner. | 2014 | 24552311 |
| clinico-microbiological profile of chronic pulmonary aspergillosis from a tertiary care centre in southern india. | pulmonary aspergillosis is commonly seen in immunocompromised individuals. a significant rise has been seen in these cases in the past decade, owing to growing number of patients with impaired immune status. | 2013 | 24551620 |
| susceptibility breakpoints and target values for therapeutic drug monitoring of voriconazole and aspergillus fumigatus in an in vitro pharmacokinetic/pharmacodynamic model. | although voriconazole reached the bedside 10 years ago and became the standard care in the treatment of invasive aspergillosis, reliable clinical breakpoints are still in high demand. moreover, this has increased due to the recent emergence of azole resistance. | 2014 | 24550381 |
| nadph oxidase promotes neutrophil extracellular trap formation in pulmonary aspergillosis. | nadph oxidase is a crucial enzyme in antimicrobial host defense and in regulating inflammation. chronic granulomatous disease (cgd) is an inherited disorder of nadph oxidase in which phagocytes are defective in generation of reactive oxidant intermediates. aspergillus species are ubiquitous, filamentous fungi, which can cause invasive aspergillosis, a major cause of morbidity and mortality in cgd, reflecting the critical role for nadph oxidase in antifungal host defense. activation of nadph oxid ... | 2014 | 24549323 |
| an ionic liquid tolerant cellulase derived from chemically polluted microhabitats and its application in in situ saccharification of rice straw. | a cellulase-producing fungus was isolated from chemically polluted microhabitats by [amim][cl] enrichment and identified as aspergillus fumigatus. the maximum activity of the cellulase in 30% (v/v) ionic liquids (ils) was detected in [emim][dmp], [amim][cl] and [emim][ma] as 127%, 111% and 109%, respectively, of its activity in buffer, suggesting its superior performance in high concentration ils. strikingly, although its initial activity varied in each il, its half-life was longer in most ils t ... | 2014 | 24549238 |
| use of denaturing high-performance liquid chromatography (dhplc) to characterize the bacterial and fungal airway microbiota of cystic fibrosis patients. | the aim of this study was to evaluate the use of denaturing high-performance liquid chromatography (dhplc) to characterize cystic fibrosis (cf) airway microbiota including both bacteria and fungi. dhplc conditions were first optimized using a mixture of v6, v7 and v8 region 16s rrna gene pcr amplicons from 18 bacterial species commonly found in cf patients. then, the microbial diversity of 4 sputum samples from 4 cf patients was analyzed using cultural methods, cloning/sequencing (for bacteria o ... | 2014 | 24535743 |
| pyrrolo[1,2-α][1,4]benzodiazepines show potent in vitro antifungal activity and significant in vivo efficacy in a microsporum canis dermatitis model in guinea pigs. | pyrrolo[1,2-α][1,4]benzodiazepines (pbds) have been described as a novel class of antifungal compounds with activity against dermatophytes and aspergillus fumigatus. the initial structure-activity relationship showed that compounds with a chlorine substitution at position 7 have a higher activity compared with regioisomers or other substituents. | 2014 | 24535279 |
| arg279 is the key regulator of coenzyme selectivity in the flavin-dependent ornithine monooxygenase sida. | siderophore a (sida) is a flavin-dependent monooxygenase that catalyzes the nad(p)h- and oxygen-dependent hydroxylation of ornithine in the biosynthesis of siderophores in aspergillus fumigatus and is essential for virulence. sida can utilize both nadph or nadh for activity; however, the enzyme is selective for nadph. structural analysis shows that r279 interacts with the 2'-phosphate of nadph. to probe the role of electrostatic interactions in coenzyme selectivity, r279 was mutated to both an a ... | 2014 | 24534646 |
| abpa concomitantly occurring with invasive sinus aspergillosis: a short report on two patients. | allergic bronchopulmonary aspergillosis (abpa) is a pulmonary disorder that is caused by hypersensitivity to aspergillus fumigatus with resultant systemic immune activation, chronic asthma, recurrent pulmonary infiltrates, and bronchiectasis. first described by hinson et al. (thorax 7:317-333 [1]) in 1952, this disease occurs in 1-2% of patients with persistent asthma and in 2-15% of patients with cystic fibrosis (greenberger, front biosci 8:s119-s127 [2]). although the diagnostic criteria for a ... | 2014 | 24533410 |
| impact of a transposon insertion in phzf2 on the specialized metabolite production and interkingdom interactions of pseudomonas aeruginosa. | in microbiology, gene disruption and subsequent experiments often center on phenotypic changes caused by one class of specialized metabolites (quorum sensors, virulence factors, or natural products), disregarding global downstream metabolic effects. with the recent development of mass spectrometry-based methods and technologies for microbial metabolomics investigations, it is now possible to visualize global production of diverse classes of microbial specialized metabolites simultaneously. using ... | 2014 | 24532776 |
| aspergillus pacemaker lead endocarditis. | a 47-year-old man who had a pacemaker implanted 2 years earlier, recently developed a fever and had been on antibiotics for 2 months. he presented with pulmonary emboli, and underwent lead extraction and emboli removal. histopathology demonstrated aspergillus. amphotericin b was continued postoperatively. this rare case of pacemaker lead endocarditis suggests that vigorous medical and surgical intervention can be curative. | 2013 | 24532625 |
| impact of scedosporium apiospermum complex seroprevalence in patients with cystic fibrosis. | species of the scedosporium apiospermum complex (s. a complex) are emerging fungi responsible for chronic airway colonization in cystic fibrosis (cf) patients. recent studies performed on aspergillus fumigatus suggest that the colonization of the airways by filamentous fungi may contribute to the progressive deterioration of lung function. | 2014 | 24530191 |
| temperature-dependent changes in the microbial storage flora of birch and spruce sawdust. | sawdust can be used to make pellets (biofuel) and particle boards and as a potential lignocellulose feedstock in bioethanol production. microbial activity can affect sawdust quality; hence, we monitored the microbial population in birch- and spruce sawdust after 3 months' storage at various temperatures. species composition was similar on both materials but was strongly influenced by temperature. bacteria were present on all materials at all conditions: on birch, 2.8 × 10(8) , 1.1 × 10(8) , and ... | 2014 | 24527731 |
| production of fungal amylases using cheap, readily available agriresidues, for potential application in textile industry. | the study aimed at isolation and screening of fungal amylase producer, optimization of solid state fermentation conditions for maximum amylase production by the best amylase producer, and characterization of the crude amylases, so produced. aspergillus fumigatus ntcc1222 showed the highest amylase activity (164.1 u/ml) in secondary screening under ssf conditions and was selected for further studies. the test strain showed maximum amylase production (341.7 u/ml) and supernatant protein concentrat ... | 2014 | 24527439 |
| hyaluronan deposition and co-localization with inflammatory cells and collagen in a murine model of fungal allergic asthma. | allergic asthma is a chronic inflammatory disease of the airways characterized by excessive inflammation and remodeling of the extracellular matrix (ecm) and associated cells of the airway wall. under inflammatory conditions, hyaluronan (ha), a major component of the ecm, undergoes dynamic changes, which may in turn affect the recruitment and activation of inflammatory cells leading to acute and chronic immunopathology of allergic asthma. | 2014 | 24519432 |
| isolation of a new broad spectrum antifungal polyene from streptomyces sp. mtcc 5680. | a new polyene macrolide antibiotic pn00053 was isolated from the fermentation broth of streptomyces sp. wild-type strain mtcc-5680. the producer strain was isolated from fertile mountain soil of naldehra region, himachal pradesh, india. the compound pn00053 was purified through various steps of chromatographic techniques and bio-activity guided fractionation followed by its characterization using physiochemical properties, spectral data ((1) h-nmr, (13) c-nmr, hmbc, hsqc, and cosy) and ms analys ... | 2014 | 24517845 |
| susceptibility breakpoints for amphotericin b and aspergillus species in an in vitro pharmacokinetic-pharmacodynamic model simulating free-drug concentrations in human serum. | although conventional amphotericin b was for many years the drug of choice and remains an important agent against invasive aspergillosis, reliable susceptibility breakpoints are lacking. three clinical aspergillus isolates (aspergillus fumigatus, aspergillus flavus, and aspergillus terreus) were tested in an in vitro pharmacokinetic-pharmacodynamic model simulating the biphasic 24-h time-concentration profile of free amphotericin b concentrations in human serum with free peak concentrations (fcm ... | 2014 | 24514094 |
| chronic granulomatous invasive fungal sinusitis: an evolving approach to management. | chronic granulomatous invasive fungal sinusitis (cgifs) is rare and a consensus on ideal management is lacking. we present an extensive case managed successfully with a conservative approach. | 2014 | 24510508 |
| plant latex: a promising antifungal agent for post harvest disease control. | bioactive compounds from plant latex are potential source of antifungic against post harvest pathogens. latex from a total of seven plant species was investigated for its phytochemical and antifungal properties. six fungi namely aspergillus fumigatus, a. niger, a. terreus, f. solani, p. digitatum and r. arrhizus were isolated from infected fruits and vegetables and tested against various solvent extracts of latex. analysis of latex extracts with phytochemical tests showed the presence of alkaloi ... | 2013 | 24506041 |
| zno nanoparticles induced exopolysaccharide production by b. subtilis strain jct1 for arid soil applications. | zno nanoparticle induced exopolysaccharide (eps) production from bacillus subtilis strain jct1 (ncbi genbank accession no. jn194187) is a novel approach for arid soil applications. in the series of investigations, environmentally benign protocol was followed for the synthesis of zno nanoparticles using extracellular enzymes obtained from aspergillus fumigatus tfr8. putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemen ... | 2014 | 24502924 |
| inhibition of nucleotide biosynthesis potentiates the antifungal activity of amphotericin b. | the polyene antifungal agent amphotericin b exhibits potent and broad spectrum fungicidal activity. however, high nephrotoxicity can hinder its administration in resource poor settings. quantification of early fungicidal activity in studies of hiv patients with cryptococcosis demonstrate that 5-fluorocytosine therapy in combination with amphotericin b results in faster clearance than with amphotericin b alone. in vitro synergy between the two drugs has also been reported but the mechanism by whi ... | 2014 | 24498052 |
| aspergillus fumigatus infection on a home-made tattoo. | 2014 | 24495231 | |
| synthesis, antifungal activities and molecular docking studies of novel 2-(2,4-difluorophenyl)-2-hydroxy-3-(1h-1,2,4-triazol-1-yl)propyl dithiocarbamates. | a series of 2-(2,4-difluorophenyl)-2-hydroxy-3-(1h-1,2,4-triazol-1-yl)propyl dithiocarbamates as new analogs of fluconazole were synthesized and their antifungal activities were evaluated. among these compounds, 2a-f and 3a-q exhibited higher activities than fluconazole against nearly all fungi tested except aspergillus fumigatus. noticeably, the in vitro biological activities of 2b, 3a, 3c, 3h-k, and 3o-q against candida species were much better than those of fluconazole and ketoconazole. also, ... | 2014 | 24487187 |
| prevalence and molecular characterization of azole resistance in aspergillus spp. isolates from german cystic fibrosis patients. | aspergillus spp. are the most frequently isolated filamentous fungi in the sputum of patients with cystic fibrosis (cf). resistance to the azoles, the mainstay of current antifungal therapy, has been increasingly observed worldwide, but few data are available on the resistance of aspergillus spp. in german cf patients. this study investigated the epidemiology of aspergillus spp. and the molecular origin of azole resistance in a large german cf centre. | 2014 | 24486872 |
| the distribution of aspergillus spp. opportunistic parasites in hives and their pathogenicity to honey bees. | stonebrood is a disease of honey bee larvae caused by fungi from the genus aspergillus. as very few studies have focused on the epidemiological aspects of stonebrood and diseased brood may be rapidly discarded by worker bees, it is possible that a high number of cases go undetected. aspergillus spp. fungi are ubiquitous and associated with disease in many insects, plants, animals and man. they are regarded as opportunistic pathogens that require immunocompromised hosts to establish infection. mi ... | 2014 | 24485932 |
| atypical presentation of allergic bronchopulmonary aspergillosis: an unusual cause of difficult-to-treat asthma. | allergic bronchopulmonary aspergillosis (abpa) commonly presents with persistently uncontrolled asthma, despite of the therapy with highest possible anti-asthma medications. most common cause of abpa is aspergillus fumigates. hence, abpa is one of the important differential diagnoses of difficult-to-treat asthma. atypical presentation of abpa misleads the diagnosis and asthma remains uncontrolled. here we present such a case of 28-year-old non-smoker, normotensive male office worker who presente ... | 2013 | 24479057 |
| pathogenesis and clinical features of chronic pulmonary aspergillosis - is it possible to distinguish cnpa and ccpa clinically? | the pathogenesis of chronic pulmonary aspergillosis (cpa) including chronic necrotizing pulmonary aspergillosis (cnpa), chronic cavitary pulmonary aspergillosis (ccpa), and simple aspergilloma (sa) has been poorly investigated. we examined all types of cpa cases with histopathological evidence to clarify the differences in pathogenesis and clinical features. | 2014 | 24477329 |
| the immune profile associated with acute allergic asthma accelerates clearance of influenza virus. | asthma was the most common comorbidity in hospitalized patients during the 2009 influenza pandemic. for unknown reasons, hospitalized asthmatics had less severe outcomes and were less likely to die from pandemic influenza. our data with primary human bronchial cells indicate that changes intrinsic to epithelial cells in asthma may protect against cytopathology induced by influenza virus. to further study influenza virus pathogenesis in allergic hosts, we aimed to develop and characterize murine ... | 2017 | 24469764 |
| synthesis and antifungal activity of asp9726, a novel echinocandin with potent aspergillus hyphal growth inhibition. | the synthesis and antifungal activity of asp9726, a novel echinocandin with potent aspergillus hyphal growth inhibition and significantly improved mic against candida parapsilosis and echinocandin resistant-candida is described. | 2014 | 24468413 |
| genetic and virulence variation in an environmental population of the opportunistic pathogen aspergillus fumigatus. | environmental populations of the opportunistic pathogen aspergillus fumigatus have been shown to be genotypically diverse and to contain a range of isolates with varying pathogenic potential. in this study, we combined two rapd primers to investigate the genetic diversity of environmental isolates from manchester collected monthly over 1 year alongside dublin environmental isolates and clinical isolates from patients. rapd analysis revealed a diverse genotype, but with three major clinical isola ... | 2014 | 24464798 |
| biodegradation of phenol by antarctic strains of aspergillus fumigatus. | taxonomic identification of three newly isolated antarctic fungal strains by their 18s rdna sequences revealed their affiliation with aspergillus fumigatus. phenol (0.5 g/l) as the sole carbon source was completely degraded by all strains within less than two weeks. intracellular activities of three key enzymes involved in the phenol catabolism were determined. activities of phenol hydroxylase (ec 1.14.13.7), hydroquinone hydroxylase (ec 1.14.13.x), and catechol 1,2-dioxygenase (ec 1.13.11.1) va ... | 2014 | 24459772 |
| aspergillus nidulans cell wall composition and function change in response to hosting several aspergillus fumigatus udp-galactopyranose mutase activity mutants. | deletion or repression of aspergillus nidulans ugma (anugma), involved in galactofuranose biosynthesis, impairs growth and increases sensitivity to caspofungin, a β-1,3-glucan synthesis antagonist. the a. fumigatus ugma (afugma) crystal structure has been determined. from that study, afugma mutants with altered enzyme activity were transformed into anugma▵ to assess their effect on growth and wall composition in a. nidulans. the complemented (anugma::wild type afugma) strain had wild type phenot ... | 2014 | 24454924 |
| [primitive aspergillosis of the posterior cerebral fossa in immunocompetent patient]. | cerebral aspergillosis arises in the great majority of cases during an invasive aspergillosis with hematogene scattering from the lung hurts. the cerebral, not rare location is one of the worse criteria forecast during the invasive aspergillosis. we report the case of patient who was hospitalized in the neurosurgery department for syndrome of increased intracranial pressure, hemiparesis and cerebellar syndrome in febrile context. the radiological exploration objectified a collection of the poste ... | 2011 | 24451505 |
| four ardeemin analogs from endophytic aspergillus fumigatus sps-02 and their reversal effects on multidrug-resistant tumor cells. | four ardeemin derivatives, 5-n-acetylardeemin (1), 5-n-acetyl-15bβ-hydroxyardeemin (2), 5-n-acetyl-15b-didehydroardeemin (3), and 5-n-acetyl-16α-hydroxyardeemin (4), were isolated from the fermentation broth of an endophytic aspergillus fumigatus sps-02 associated with artemisia annua l. the structures of these metabolites were elucidated by a combination of spectroscopic data, including 1d-, 2d-nmr and ms. in vitro chemosensitization assay indicated that these ardeemins had different activities ... | 2014 | 24443428 |
| ph signaling in human fungal pathogens: a new target for antifungal strategies. | fungi are exposed to broadly fluctuating environmental conditions, to which adaptation is crucial for their survival. an ability to respond to a wide ph range, in particular, allows them to cope with rapid changes in their extracellular settings. pacc/rim signaling elicits the primary ph response in both model and pathogenic fungi and has been studied in multiple fungal species. in the predominant human pathogenic fungi, namely, candida albicans, aspergillus fumigatus, and cryptococcus neoforman ... | 2014 | 24442891 |
| distribution, expression and expansion of aspergillus fumigatus line-like retrotransposon populations in clinical and environmental isolates. | functional genomic analysis of the mould pathogen aspergillus fumigatus has identified multiple secondary metabolism genes upregulated in the host niche. intriguingly, transcriptomic analyses of infectious germlings, germinating spores and mutants lacking the laea methyltransferase reveal differential expression of transposable elements (tes), which often flank secondary metabolite gene clusters. in this study we investigate, in clinical and environmental isolates, the structure and distribution ... | 2014 | 24440682 |
| in vitro susceptibility of filamentous fungal isolates from a corneal ulcer clinical trial. | to describe the minimum inhibitory concentration (mic) of fungal isolates to natamycin and voriconazole, and to compare these mics to previous ocular susceptibility studies. | 2014 | 24439440 |