Publications

the use of fluorescein-labelled lectins in the detection and identification of fungi pathogenic for man: a preliminary study.	fluorescein-labelled lectins of known specificities for different sugars were used in an attempt to identify fungi in paraffin sections of surgical and post-mortem material. aspergillus fumigatus, blastomyces dermatitidis, candida albicans, cryptococcus neoformans, paracoccidioides brasiliensis and rhizopus oryzae have been studied with five fluorescein-labelled lectins and with basis of differences in their reactions with these stains. the results accord well with what is known of the chemistry ...	1978	355633
paracoccidioides brasiliensis: cell wall structure and virulence. a review.	paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis or south american blastomycosis. many aspects of the disease and its agent are unknown. one of the most important factors regarding the infection and the host-parasite relationships seems to be the fungal cell wall whose biochemical aspects are reviewed here. biochemical studies, done mainly by kanetsuna et al., (21,22), have demonstrated that the yeastlike (y) and the mycelial (m) forms have chitin as a common polysa ...	1977	340954
new method for estimating digestion of paracoccidioides brasiliensis by phagocytic cells in vitro.	we describe a method by which phagocytosis and digestion of paracoccidioides brasiliensis yeast cells by polymorphonuclear leukocytes or other phagocytic cells may be estimated. suspensions of p. brasiliensis in its yeastlike phase were sonicated, counted, and incubated with known numbers of peripheral blood polymorphonuclear leukocytes. at given intervals, cytocentrifuge droplets were stained by a variation of papanicolaou's method. stained preparations were examined with phase-contrast optics. ...	1979	90683
[study of serologic cross reactions between the antigens of paracoccidioides brasiliensis and histoplasma capsulatum].	a comparative study of serologic cross-reactions between histoplasmosis and paracoccidioidomycosis was carried out using complement fixation (cf) and agar gel immunodiffusion (id) tests. sera from 77 patients with paracoccidioidomycosis, 38 with histoplasmosis and 50 healthy individuals were studied. the pan american health organization microtiter cf test and the hupper id technique were employed. the antigens were prepared by the method of ajello as modified by negroni. paracoccidioidin cf test ...	1976	63979
[reactivity of a purified antigen of paracoccidioides brasiliensis with paracoccidioidomycosis and histoplasmosis sera].	the immunoelectrophoretic reactivity of a "crude" and a "purified" antigens of paracoccidiodes brasiliensis against the sera of confirmed cases of paracoccidioidomycosis and histoplasmosis was evaluated. the "crude" antigen revealed precipitating antibodies in both the homologous and the heterologous human sera; the number of precipitating systems being higher with the former. the "purified" antigen reacted only with the homologous sera. it is postulated that the use of such "purified" antigen m ...	1975	55090
beta-1-3-glucanase and dimorphism in paracoccidioides brasiliensis.	mycelial and yeast forms of p. brasiliensis were tested for several glucohydrolases. in addition to high levels of beta-glucanases, low amounts of alpha-glucanase, chitinase and maltase were found. tests for invertase, amylase and lactase were negative. the levels of beta-1,3-glucanase were higher in the mycelial form. the shift to the mycelial phase correlated with an increase in the levels of beta-1,3-glucanase. the enzyme was present in the cytoplasm, cell wall and culture medium. the extrace ...	1979	40530