Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| childhood brucellosis in israel. | brucellosis has become a major medical problem in israel particularly in the muslim arab population. | 1996 | 8823856 |
| infected abdominal aortic aneurysm caused by spinal brucellar infection. | 1996 | 8819420 | |
| infective endocarditis and occult splenic abscess caused by brucella melitensis infection: a case report and review of the literature. | this report describes a patient with prosthetic aortic valve endocarditis caused by brucella melitensis infection with an initial relapse after apparent recovery due to associated occult splenic abscess. in spite of a prolonged period of recovery (6 months) achieved with splenectomy and antibrucella antimicrobial therapy, a second relapse occurred and cure was achieved after replacement of the infected prosthetic valve and prolonged courses of antibrucella antimicrobial therapy. there is controv ... | 1996 | 8817011 |
| [isolation of brucella melitensis biovar 2 from the human milk]. | 1995 | 8814668 | |
| thrombosis of the abdominal aorta secondary to brucella spondylitis. | spondylitis is one of the most frequent osteoarticular complications of brucella infection in adults. occasionally it gives rise to soft tissue paravertebral or epidural masses, which can compress surrounding structures. a patient with thrombosis of the abdominal aortic artery secondary to brucella lumbar spondylitis is presented here, a complication that has not been previously reported. | 1996 | 8811368 |
| production and characterisation of monoclonal antibodies to brucella melitensis cytosoluble proteins that are able to differentiate antibody responses of infected sheep from rev. 1 vaccinated sheep. | monoclonal antibodies (mabs) were produced to brucella melitensis cytosoluble proteins (cp) with apparent molecular masses of 12, 24 and 28 kda (cp12, cp24 and cp28) which were previously shown by immunoblotting to differentiate antibody responses of infected sheep from those of b. melitensis strain rev. 1 vaccinated sheep. these mabs were derived from mice infected with virulent smooth (s) b. melitensis strain h38. most mabs obtained were directed to cp28, which indicated (as was shown in infec ... | 1996 | 8810948 |
| polymorphism at the dnak locus of brucella species and identification of a brucella melitensis species-specific marker. | the dnak gene and surrounding sequences from reference strains of the six brucella species were amplified by the polymerase chain reaction (pcr) with primers chosen according to the published sequence of the b. ovis dnak gene and studied for polymorphism with nine restriction endonucleases. the restriction patterns were identical for all species with all restriction endonucleases tested except for b. melitensis strain 16m that showed a different pattern with ecorv, consistent with the presence o ... | 1996 | 8810947 |
| effect of antibiotics contained in two brucella selective media on growth of brucella abortus, b. melitensis, and b. ovis. | the mic and the highest concentration enabling bacterial growth (ceg) of the antibiotics contained in two selective media were determined for brucella abortus, b. melitensis, and b. ovis. the nalidixic acid and bacitracin contained in farrell's selective medium were responsible for the inhibitory effects observed. | 1996 | 8789029 |
| brucella melitensis biotype 1 outbreak in goats in northern kwazulu-natal. | 1996 | 8786610 | |
| a brucella melitensis high temperature requirement a (htra) deletion mutant demonstrates a stress response defective phenotype in vitro and transient attenuation in the balb/c mouse model. | bacterial stress response proteins of the high temperature requirement a (htra) family are serine proteases which appear to play an important role in scavenging oxidatively damaged proteins from the cell before they reach toxic levels. an isogenic htra deletion mutant, designated rwp5, was constructed from virulent brucella melitensis 16m via gene replacement to determine whether the b. melitensis htra protein functions as a stress response protein, and to evaluate the contribution of this prote ... | 1995 | 8778563 |
| brucella melitensis can be transmitted sexually. | 1996 | 8774592 | |
| [a seroepidemiological study of brucellosis infection in the province of campobasso]. | these research regarded a seroepidemiological study of brucellosis in the campobasso province. the results related to 204 subjects (96 males and 108 females) pointed out the 17.15% of subjects were positive for antibodies anti-brucella, 28.6% of these were positive for br. melitensis. the results agree with the data of the literature. | 1996 | 8766351 |
| cloning, nucleotide sequence, and expression of the brucella melitensis bp26 gene coding for a protein immunogenic in infected sheep. | we have previously identified a brucella melitensis 28 kda cytosoluble protein (cp28) which was highly immunogenic in infected sheep and which in addition made possible the serological differentiation between infected and b. melitensis rev. 1 vaccinated sheep. monoclonal antibodies against cp28 were used to screen a b. melitensis 16m genomic library and to clone the corresponding gene. dna sequencing of the gene encoding cp28 of b. melitensis 16m revealed that it was nearly identical to that of ... | 1996 | 8764475 |
| overproduction of the brucella melitensis heat shock protein dnak in escherichia coli and its localization by use of specific monoclonal antibodies in b. melitensis cells and fractions. | the brucella melitensis dnak gene was amplified by the polymerase chain reaction using primers chosen according to the published sequence of b. ovis and cloned in multiple copy plasmids enabling expression under the control of the plac promoter. monoclonal antibodies (mab) obtained by immunizing mice with b. melitensis b115 cell wall (cw) fraction or by infecting mice with virulent b. melitensis strain h38 and recognizing a 73-kda band in immunoblotting of the b. melitensis cw fraction reacted w ... | 1996 | 8761733 |
| cloning, nucleotide sequence, and expression of the brucella melitensis omp31 gene coding for an immunogenic major outer membrane protein. | the gene coding for the major outer membrane protein (omp) of 31 to 34 kda, now designated omp31, of brucella melitensis 16m was cloned and sequenced. a b. melitensis 16m genomic library was constructed in lambda gem-12 xhoi half-site arms, and recombinant phages expressing omp31 were identified by using the anti-omp31 monoclonal antibody (mab) a59/10f09/g10. subcloning of insert dna from a positive phage into pgem-7zf allowed the selection of a plasmid bearing a 4.4-kb ecori fragment that seeme ... | 1996 | 8751924 |
| childhood brucellosis in north-western greece: a retrospective analysis. | fifty-two cases of childhood brucellosis which occurred in north-western greece during the 15-year period 1979-1993, are reviewed. it is believed that they represent very closely the total incidence of the disease in the region which has a population of 100,000 children aged 0-14 years old. brucellosis-affected children were almost exclusively from goat- or shepherd families and of both sexes and all age groups. a broad spectrum of clinical manifestations ranging from malaise only to brain absce ... | 1996 | 8750800 |
| comparison of two selective media for the isolation of brucella melitensis from naturally infected sheep and goats. | the efficacy of two selective media for the isolation of brucella melitensis from naturally infected sheep and goats was compared. two hundred and eighty sheep and 60 goats belonging to b melitensis-infected flocks were slaughtered and samples of milk (when available) and seven tissues were taken from each animal for bacteriological analyses. a modified thayer-martin's medium was more sensitive than farrell's medium; by the combined use of both media 142 infected sheep and 40 infected goats were ... | 1996 | 8733178 |
| protection of balb/c mice against homologous and heterologous species of brucella by rough strain vaccines derived from brucella melitensis and brucella suis biovar 4. | to evaluate stable rough mutants derived from brucella melitensis 16m and b suis 2579 (biovar 4) as vaccines against homologous and heterologous brucella spp in the balb/c mouse model. design, animals, and procedure: rough mutants vtrm1 and vtrs1 were obtained from b melitensis 16m and b suis 2579, respectively, by allelic exchange of rfbu gene encoding mannosyltransferase with a tn5-disrupted rfbu gene. mice were vaccinated with vtrm1 or vtrs1 and challenge exposed 8 weeks later. | 1996 | 8723881 |
| a comparison of the potency of several brucella allergens used to detect brucellosis in cattle. | the potency of brucella allergens prepared from a smooth brucella abortus strain s-99, mucoid strain leewarden, rough strain 45/20, and rough brucella melitensis strain b-115 was assessed. the potency of these allergens was compared with that of a standard allergen prepared from smooth brucella abortus s-99 that efficiently detected bovine brucellosis in other studies. eight cattle experimentally inoculated with brucella abortus 544 were tested with the allergens 4 and 10 weeks after infection, ... | 1996 | 8711894 |
| enzyme-linked immunosorbent assay with partially purified cytosoluble 28-kilodalton protein for serological differentiation between brucella melitensis-infected and b. melitensis rev.1-vaccinated sheep. | the problem of differentiating sheep infected with brucella melitensis from those vaccinated or exposed to cross-reaching organisms has not been resolved by conventional serological tests or through the use of the smooth lipopolysaccharide in primary binding assays. we therefore analyzed sera from ewes experimentally infected with b. melitensis h38, from ewes naturally infected with b. melitensis, and from b. melitensis rev.1-vaccinated ewes by enzyme-linked immunosorbent assay with three antige ... | 1996 | 8705674 |
| complications associated with brucella melitensis infection: a study of 530 cases. | we carried out a prospective study of 530 patients older than 14 years of age with brucellosis. we describe the incidence and clinical features of the focal forms of the disease, analyzing some of the possible factors associated with their appearance. one hundred sixty-nine patients (31.9%) had a focal form or complication. osteoarticular complications were the most frequent, totaling 113 cases (66%), followed by genitourinary with 18 cases (5.1% of males), hepatic (2.5%), neurologic (1.7%), and ... | 1996 | 8699960 |
| cloning of a brucella melitensis group 3 antigen gene encoding omp28, a protein recognized by the humoral immune response during human brucellosis. | brucella group 3 antigens (ags) are outer membrane proteins (omps) with a molecular mass ranging from 25 to 30 kda. the omps are of interest partially because of their potential use as vaccine and diagnostic reagents. we used human convalescent antibody (ab) to clone a gene that encoded a 28-kda protein from a lambdagt11 library of brucella melitensis 16m genomic dna. dna sequence analysis revealed a single open reading frame that would encode a protein of 26,552 da. the 28-kda protein had a pri ... | 1996 | 8698471 |
| bacterial persistence and immunity in goats vaccinated with a pure deletion mutant or the parental 16m strain of brucella melitensis. | to evaluate host responses, young goats were inoculated subcutaneously with a genetic deletion mutant (deltapure201) of brucella melitensis (n = 6), its virulent parental strain 16m (n = 6), or saline (n = 6). no clinical evidence of brucellosis was seen in any goat. serum antibody titers peaked at postinoculation day (pid) 14. bacteria in lymph nodes that drained sites of vaccination reached peak numbers of >10(6) cfu/g in both infected groups at pid 7 and progressively declined to pid 84. at n ... | 1996 | 8698463 |
| agglutinins for brucellae antigens in blood sera of an urban and rural population in kenya. | the antibody titres for brucella arbotus and br. melitensis in 364 sera from healthy individuals in nairobi and naivasha are presented. a majority (96%) had no detectable agglutinins. reactivity was markedly higher in the naivasha serum samples, than in those from nairobi. in naivasha, seven per cent showed reactivity, whereas in nairobi a larger majority (98%), showed no reactivity, with antibody titres ranging from 1:20-1:160. age and sex were found to have no effect on antibody titre distribu ... | 1996 | 8698024 |
| a case of brucella melitensis endocarditis successfully treated by a combination of surgical resection and antibiotics. | endocarditis is a rare complication of brucellosis, often fatal, and the treatment is controversial. we present a case of brucella melitensis endocarditis successfully treated by a combination of surgical resection and antibiotics. | 1995 | 8685649 |
| deletion of pure attenuates brucella melitensis infection in mice. | we previously showed that a pure mutant (delta pure201) of brucella melitensis 16m is attenuated for growth in cultured human monocytes (e. s. drazek, h. h. houng, r. m. crawford, t. l. hadfield, d. l. hoover, and r. l. warren, infect. immun. 63:3297-3301, 1995). to determine if this strain is attenuated in animals, we compared the growth of the delta pure201 mutant with that of strain 16m in balb/c mice. the number of bacteria in the spleen and spleen weight peaked for both strains between 1 an ... | 1996 | 8675325 |
| nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of brucella ovis: evidence for antigenic shift, compared with other brucella species, due to a deletion in the gene. | the nucleotide sequences encoding the major 25-kda outer membrane protein (omp) (omp25 genes) of brucella ovis 63/290, brucella melitensis 16m, brucella suis 1330, brucella canis rm6/66, and brucella neotomae 5k33 (all reference strains) were determined and compared with that of brucella abortus 544 (p. de wergifosse, p. lintermans, j. n. limet, and a. cloeckaert, j. bacteriol. 177:1911-1914, 1995). the major difference found was between the omp25 gene of b. ovis and those of the other brucella ... | 1996 | 8675306 |
| [brucella pancarditis with fatal outcome]. | brucella endocarditis was diagnosed in a 21-year-old itinerant farm worker hospitalized for acute pulmonary edema. history taking revealed cough, fever and sweating one month earlier which had been treated with antibiotics. at admission, echography showed lesions on the aortic valve and hemocultures identified brucella meltensis. on day 7 of specific treatment with doxycycline (200 mg/day) and rifamycine (1200 mg/day), and despite digitalics and diuretics, left ventricular failure rapidly worsen ... | 1996 | 8668692 |
| clinical and therapeutic features of childhood neurobrucellosis. | brucellosis is a multisystem disease with diverse clinical presentations, and involvement of the nervous system is considered to be rare in childhood. five children with meningitis (n=2), meningoencephalitis (n=1), meningomyelitis (n=1), or cerebellar ataxia (n=1) are described, all of whom had a history of exposure to a possible source of brucellosis. examination of cerebrospinal fluid (csf) revealed lymphocytic pleocytosis in 4 patients, high protein concentration in 5 and low glucose concentr ... | 1995 | 8658066 |
| brucella melitensis--a sexually transmissible agent? | 1996 | 8656919 | |
| control of brucella melitensis infection in a large camel herd in saudi arabia using antibiotherapy and vaccination with rev. 1 vaccine. | the authors describe an attempt to control brucella melitensis infection in a large camel herd in saudi arabia. sera from the entire herd (2,536) were examined by the rose bengal and standard united states of america buffered plate agglutination tests. the overall brucella seroprevalence was 8%. milk samples from the 120 seropositive milking camels were cultured on brucella-selective media. b. melitensis biovars 1, 2 and 3 were isolated from 41 camels (34%). seropositive camels (202) were treate ... | 1995 | 8593404 |
| comparative activity of azithromycin and doxycycline against brucella spp. infection in mice. | the activities of a short therapeutic regimen with azithromycin and the classic treatment doxycycline with streptomycin were compared and evaluated in mice infected with brucella melitensis. in a chronic model, starting therapy 31 days after challenge, azithromycin (10 days, 50 mg/kg/day) significantly reduced the infection (2.9 logs, day 48 post-infection). the effectiveness of doxycycline (21 days, 50 mg/kg/12 hourly) was greater than azithromycin (4.1 logs of reduction, day 48 post-infection) ... | 1995 | 8591939 |
| antibiotic treatment induces an increase of the specific antibody levels in brucella melitensis infected mice. | the effects of doxycycline (dox) and streptomycin (sm) treatment of brucella melitensis infected mice on humoral immune response were examined. in female balb/c mice, dox was administered at a dose of 50 mg/kg/12 h, for 21 or 45 consecutive days, alone or combined with sm (10 mg/kg/12 h) for 14 days. all treatments induced a significant increase in specific igg levels (elisa and ciep) against lps and cytosolic antigens of brucella during treatment. this was not related with therapeutic failure o ... | 1995 | 8589668 |
| [the epidemiological characteristics of brucellosis in the region of the northern caucasus]. | at present, the northern caucus region is the most unfavourable brucellosis area of the russian federation where 43.5% of the total russian incidence of this infection was recorded in 1993. over the past years (1988-1993) there was a highly unsteady-state improvement of the epidemiological situation, however, the rates of human morbidity with this infection greatly lag behind those in russia. the active foci of small cattle brucellosis as the leading source of human contamination with brucellosi ... | 1995 | 8587517 |
| single-step pcr for detection of brucella spp. from blood and milk of infected animals. | a versatile method for the extraction of brucella dna and pcr are presented as reliable tools for the detection of brucella spp. from body fluids of infected animals. two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. the sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in ... | 1995 | 8586678 |
| acute pancreatitis associated with brucellosis. | acute pancreatitis developed in a patient with acute brucellosis due to brucella melitensis. to the best of our knowledge this is only the second reported case of acute pancreatitis associated with brucella infection. | 1995 | 8580415 |
| characterization of brucella abortus and brucella melitensis native haptens as outer membrane o-type polysaccharides independent from the smooth lipopolysaccharide. | brucella native haptens (nhs) extracted with hot water from smooth (s)-type b. abortus and b. melitensis were purified to high levels of serological activity and compared with the polysaccharide obtained by acid hydrolysis (ps) of the s lipopolysaccharide (s-lps). by 13c nuclear magnetic resonance analysis, nhs showed the spectrum of a homopolymer of alpha-1,2- or alpha-1,2- plus alpha-1,3-linked 4-formamido-4,6-dideoxy-d-mannose (n-formylperosamine) previously reported for the lps o chain. howe ... | 1996 | 8576040 |
| molecular characterization, occurrence, and immunogenicity in infected sheep and cattle of two minor outer membrane proteins of brucella abortus. | screening of a brucella abortus genomic library with two sets of monoclonal antibodies allowed the isolation of the genes corresponding to two minor outer membrane proteins (omp10 and omp19) found in this bacterial species. sequence analysis of the omp10 gene revealed an open reading frame capable of encoding a protein of 126 amino acids. the nucleotide sequence of the insert producing the omp19 protein contains two overlapping open reading frames, the largest of which (177 codons) was shown to ... | 1996 | 8557326 |
| doxycycline-rifampin versus doxycycline-streptomycin in treatment of human brucellosis due to brucella melitensis. the gecmei group. grupo de estudio de castilla-la mancha de enfermedades infecciosas. | brucellosis is a common zoonosis in many parts of the world; the best regimen for the treatment of brucellosis has not been clearly determined. we have carried out a multicenter, open, controlled trial in five general hospitals in spain to compare the efficacy and safety of doxycycline and rifampin (dr) versus doxycycline and streptomycin (ds) for the treatment of human brucellosis. the study included 194 ambulatory or hospitalized patients with acute brucellosis, without endocarditis or neurobr ... | 1995 | 8540716 |
| [azithromycin, pharmacodynamic evaluation in animal models]. | several experimental models have been used in order to evaluate the in vivo efficacy of azithromycin against numerous human pathogenic bacteria and parasites, including comparison between azithromycin and other antibiotics belonging or not to the macrolide family. using the experimental models, three major objectives can be distinguished: the comparative studies of the efficacy dose 50 (ed50) of azithromycin compared to other orally given antibiotics, the azithromycin efficacy in animal infected ... | 1995 | 8539074 |
| brucellosis presenting with cough. | 1995 | 8532438 | |
| brucellar bacteremia in pregnancy. | 1995 | 8527594 | |
| immunological response in mice after long-term stimulation with cell wall antigens from brucella melitensis. | the continuous stimulation of the immune system using cell wall antigens from brucella melitensis was found to cause both quantitative and qualitative changes in circulating lymphocyte populations in mice. animals were inoculated in the hind legs with antigens on alternate days for varying lengths of time. during a two-month period, we saw a higher number of circulating lymphocytes, with an increase in the number of cd4+ cells (l3t4+) and b lymphocytes (i-ad). after two months, a drop in the ove ... | 1995 | 8525067 |
| brucellosis associated with unpasteurized milk products abroad. | 1995 | 8519642 | |
| hypothermia as a presenting sign of brucellosis. | 1993 | 8513071 | |
| [immunobiological characteristics of brucella outer membrane proteins, genetic determinants of which are expressed in escherichia coli cells]. | the influence of the brucella omp preparations obtained by the genetic engineering technique (18, 38 and 18 + 38 kd) on the formation of the specific protection and progress of infectious process in brucellosis in the in vivo experiments has been studied. the omps synthesized in escherichia coli cells gse579 and having mol mass 18 and 38 kd are common antigens for a number of brucella species. the 18 kd omp was found to protect 66.7% of experimental animals against brucellosis, while the protect ... | 1993 | 8510678 |
| isolation of brucella melitensis from human milk. | 1993 | 8505580 | |
| brucella mycotic aneurysm of ascending aorta complicating discrete subaortic stenosis. | 1993 | 8498327 | |
| brucellosis among hospital employees in saudi arabia. | to report experience with brucellosis among expatriate hospital employees in an area of the world where brucellosis is endemic. | 1993 | 8496581 |
| endocarditis caused by brucella melitensis. | to describe the management of an uncommon but serious infection in australia. | 1993 | 8479384 |
| lymphocytes bearing the gamma delta t cell receptor in acute brucella melitensis infection. | a phenotypical analysis carried out by indirect immunofluorescence and two-color cytofluorometry showed that the number of lymphocytes bearing the gamma delta t cell receptor (tcr) heterodimer was dramatically increased in the blood of six children with brucella melitensis infection. most in vivo expanded gamma delta t cells reacted with a monoclonal antibody which identifies v delta 2 gene products and a significant proportion expressed cd25 and hla-dr activation antigens. in addition, whereas ... | 1993 | 8477812 |
| [bacteremia and multiple and recurrent skin ulcers due to brucella melitensis. a new modality of self-induced infection]. | self-induced infections are a special form of factitious disease. they are characterized by the autoinoculation of contaminated substances or bacterial cultures which may lead to cutaneous infections, bacteremias, or arthritis by various germs. the conditions produced are difficult to diagnose and may lead to multiple operations, and sometimes mutilations or death of the patient. herewith, a patient who, over a period of 10 months, presented 4 episodes of bacteremia and cutaneous abscess in whic ... | 1993 | 8464259 |
| spontaneous peritonitis due to brucella melitensis in a patient with cirrhosis. | 1993 | 8462568 | |
| effect of age and duration of disease on the clinical manifestations of brucellosis. a study of 73 consecutive patients in israel. | we describe our experience with 73 patients diagnosed with brucellosis during the years 1979-91 at two jerusalem hospitals: hadassah mount scopus (37 patients from 1979-1984) and shaare zedek (36 patients from 1979-1991). the patients included 32 children less than 14 years old and 41 adults; 70 of the patients were non-jews. in all cases the pathogen was brucella melitensis. the high proportion of children and the equal sex distribution was quite different from the age and sex distribution of b ... | 1993 | 8454438 |
| [hematogenous pubic osteoarthritis]. | pubic osteoarthritis is a little known pathological entity with a very controversial etiology. we present four cases of public osteoarthritis in which the infectious etiology was hematogeneous. the causal germs were: staphilococcus aureus, pseudomona aeruginosa, mycobacterium tuberculosis and brucella melitensis. in the first three cases, a surgical approach was used, allowing us to establish the etiological diagnosis of the process and to perform the local debridement. in the case of brucellar ... | 1993 | 8448328 |
| [brucella melitensis: another possible cause of breast abscess++]. | 1993 | 8441291 | |
| chronic brucella empyema. | brucellosis rates have decreased in developed countries in recent years, but the pathologic condition still occurs in developing countries and well-known endemic areas such as the mediterranean and middle eastern countries. a portuguese patient presented in switzerland with long-term pleural empyema, which was later diagnosed as brucella empyema through positive cultures of brucella melitensis. brucellosis should be considered in patients who have traveled to middle eastern countries or any othe ... | 1993 | 8432167 |
| human brucellosis in dhofar, sultanate of oman. | sera were collected, mostly from school children, in six localities of the southern region of the sultanate of oman. macro and micro-agglutination tests were used to indicate positive brucella serology. four of the 525 sera tested had titres of at least 1:200, which were considered positive, and two had borderline values. the frequency of serologically positive sera in the six localities ranged between zero and 2%. no relevant difference was observed between titres using antigen of b. abortus or ... | 1993 | 8429574 |
| evaluation of vaccines and of antigen therapy in a mouse model for brucella ovis. | a mouse model was developed to study brucella ovis infection. the evolution of the number of b. ovis per spleen of mice inoculated intravenously, intraperitoneally or subcutaneously was found to be independent of the sex of the mice. the number of b. ovis increased in the spleen when increasing the challenge dose up to 1.7 x 10(7). at higher doses of challenge, the response remained constant. in this model it was observed that the inoculation of brucella melitensis rev 1 vaccine or subcellular b ... | 1993 | 8427038 |
| presence of two independent chromosomes in the brucella melitensis 16m genome. | mapping the restriction fragments of the brucella melitensis 16m genome with a new restriction endonuclease, paci, which cut the dna into only eight fragments, indicated that this species contains two unique and independent replicons of about 2,100 and 1,150 kb. pulsed-field gel electrophoresis of intact dna revealed two bands migrating the expected distances. these replicons were identified as two unique and independent chromosomes by the presence of rrna operons and genes for heat shock protei ... | 1993 | 8423146 |
| bone infection resembling phalangeal microgeodic syndrome in children. a case report. | phalangeal microgeodic syndrome of childhood is a rare condition and is the result of necrosis and repair within the phalanges. the cause is unknown. we present a case in which brucella melitensis was grown from one of the lesions. | 1993 | 8409665 |
| brucella group 3 outer membrane proteins contain a heat-modifiable protein. | brucella melitensis and b. ovis outer membrane blebs contained a protein displaying a temperature-dependent molecular mass upshift from 25 kda to 30 kda. a fraction of the protein tightly bound to lps did not show the molecular mass upshift which was also blocked by exposure of the protein to zwittergent 314. the b. melitensis heat-modifiable protein and escherichia coli ompa shared antigenic determinants. these data indicate that the brucella group 3 outer membrane proteins belonged to the ompa ... | 1993 | 8405955 |
| susceptibility of salmonella typhi and brucella melitensis to the new fluoroquinolone rufloxacin (mf 934). | the antimicrobial activity of the new fluoroquinolone rufloxacin (mf 934) was evaluated by a standardized agar dilution method against recent clinical isolates of salmonella typhi (67 strains) and brucella melitensis (108 isolates). the results were compared with 5 other commercially available or investigational fluoroquinolones. all the isolates of s. typhi were inhibited by 1.0 mg/l of rufloxacin as compared to 4-8 mg/l for b. melitensis. minimum inhibitory concentrations of rufloxacin against ... | 1993 | 8396527 |
| susceptibility of brucella melitensis to the new fluoroquinolone pd 131628: comparison with other drugs. | the antibacterial activity of pd 131628 was determined by the broth dilution method against 105 clinical isolates of brucella melitensis. fifty-two and ninety-nine percent of the isolates were inhibited by 0.06 and 0.12 microgram/ml of this quinolone, respectively. one strain which was resistant to 8 other fluoroquinolones, with a minimum inhibitory concentration (mic) of > 4.0 micrograms/ml, did not show cross-resistance and had an mic of 2.0 micrograms/ml. of the 9 fluoroquinolones tested, pd ... | 1993 | 8384544 |
| [pathologic features of a case of systemic brucellosis due to brucella melitensis biovar 3 in a chamois (rupicapra rupicapra)]. | a case of systemic brucellosis caused by brucella melitensis biovar 3 was identified in a chamois (rupicapra rupicapra) in the southern french alps. clinical signs included bilateral orchiepididymitis and keratoconjunctivitis, polyarthritis, and neurological signs; pathological findings confirmed an ulcerative keratitis, orchiepididymitis with calcified or necrotic foci and serofibrinous and hemorrhagic polyarthritis. the kidney cortex showed calcified nodules and infarctions. a small focus of c ... | 1993 | 8364757 |
| pancytopenia in children with brucellosis: clinical manifestations and bone marrow findings. | among 276 paediatric cases of brucellosis seen over a 7-year period, 16 patients (5.8%) with pancytopenia were identified. the most frequent presentations were fever, malaise, anorexia, weight loss, arthralgia, and hepatosplenomegaly. fourteen patients (87.5%) had positive blood and/or bone marrow cultures for brucella melitensis. bone marrow aspiration specimens showed hypercellularity in 14 patients and normocellularity in 2. histiocytes, eosinophils and plasma cells were increased in every ma ... | 1993 | 8362601 |
| crystal structure to 2.45 a resolution of a monoclonal fab specific for the brucella a cell wall polysaccharide antigen. | the atomic structure of an antibody antigen-binding fragment (fab) at 2.45 a resolution shows that polysaccharide antigen conformation and fab structure dictated by combinatorial diversity and domain association are responsible for the fine specificity of the brucella-specific antibody, yst9.1. it discriminates the brucella abortus a antigen from the nearly identical brucella melitensis m antigen by forming a groove-type binding site, lined with tyrosine residues, that accommodates the rodlike a ... | 1993 | 8358294 |
| persistent coccobacillary sepsis in a preterm newborn. | 1993 | 8345992 | |
| brucellar sacroiliitis: findings in 63 episodes and current relevance. | between 1974 and 1989 we prospectively observed 530 patients with brucellosis. the findings for 62 patients (42 males and 20 females; mean age, 34.7 years) with 63 episodes in which the sacroiliac joint was involved (the most frequent osteoarticular location [11.7%]) were analyzed. ten of the older patients (mean age, 55.3 years) had concomitant spondylitis. systemic symptoms were usually important, and characteristic pain and findings of sacroiliitis were observed in approximately 75% of cases. ... | 1993 | 8329507 |
| factors affecting detection of brucella melitensis by bactec nr730, a nonradiometric system for hemocultures. | the detection of brucella bacteremia by subculture does not always correlate with a positive signal in the bactec nr730 nonradiometric system (becton dickinson diagnostic instrument systems, sparks, md.). the effect of the inoculum size, ph, sodium polyanetholesulfonate, carbon sources (i-erythritol, sodium pyruvate, monosodium glutamate, d-glucose, and l-alanine), and urea in the release of co2 was evaluated by using the reference strain brucella melitensis 16m. in standard nr6 vials with or wi ... | 1993 | 8308111 |
| comparative analysis of brucella serotype a and m and yersinia enterocolitica o:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats. | hapten polysaccharides of brucella smooth m and a serotypes were prepared from brucella sp. and yersinia enterocolitica o:9 by previously described hydrolytic (o chain) or nonhydrolytic (native hapten [nh]) procedures. the purified polysaccharides differed only in the presence (o chain) or absence (nh) of lipopolysaccharide core sugars. the polysaccharides were compared by reverse radial immunodiffusion for the diagnosis of brucellosis in cattle (brucella abortus biotype 1 [a serotype] and bruce ... | 1993 | 8308104 |
| protective immunity to brucella ovis in balb/c mice following recovery from primary infection or immunization with subcellular vaccines. | experiments were performed with balb/c mice to elucidate the roles of humoral and cell-mediated immune responses in the acquisition of protective immunity to brucella ovis and to compare infection immunity with immunity developed through vaccination with a hot saline extract (hs) of b. ovis. mice convalescing from a primary infection with b. ovis displayed a high level of resistance to reinfection, as evidenced by splenic bacterial counts decreased over 10,000-fold from control groups at 2 weeks ... | 1994 | 8300219 |
| efficacy of brucella suis strain 2 vaccine against brucella ovis in rams. | the protective efficacy against brucella ovis of live vaccine brucella suis strain 2 (s2) and brucella melitensis strain rev 1 has been evaluated in rams. fourteen 4-month-old brucella-free aragonesa rams were vaccinated conjunctivally with 2 x 10(9) c.f.u. s2. sixteen rams of the same breed, condition and age were conjunctivally vaccinated the same day with 1.6 x 10(9) rev 1. thirteen rams were unvaccinated controls. eight months after vaccination all rams were challenged with 6 x 10(9) c.f.u. ... | 1993 | 8296481 |
| southwestern internal medicine conference: brucellosis: don't let it get your goat! | brucellosis is a highly pleomorphic zoonotic infection caused by one of the following four species of gram-negative facultative intracellular coccobacilli: brucella melitensis, b. abortus, b. suis, or b. canis. the disease is a worldwide public health problem and a significant cause of economic losses in domestic live-stock. although largely eradicated in most industrialized countries, in the united states there has been an upsurge of b. melitensis cases associated with the ingestion of unpasteu ... | 1994 | 8291510 |
| brucellosis: differential diagnosis of acute abdominal pain. | a 34-year-old turkish woman presented with septic fever, sweats, arthralgia, and abdominal pain. further examination revealed generalized lymphadenopathy, hepatosplenomegaly, pancytopenia, and multiple caseous granulomas. microbiological diagnosis revealed brucella melitensis type 3 as causative agent. this case report demonstrates that abdominal pain can be a symptom in brucellosis, and caseous granulomas may occur. in our mobile society brucella infection should be considered as possible diffe ... | 1993 | 8291280 |
| [acute brucellosis, pancytopenia and bone marrow hypoplasia]. | 1993 | 8289516 | |
| high-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gd synthesized by baculovirus-infected insect cells. | two forms of herpes simplex virus glycoprotein gd were recombined into autographa californica nuclear polyhedrosis virus (baculovirus) and expressed in infected spodoptera frugiperda (sf9) cells. each protein was truncated at residue 306 of mature gd. one form, gd-1(306t), contains the coding sequence of patton strain herpes simplex virus type 1 gd; the other, gd-1(qaat), contains three mutations which eliminate all signals for addition of n-linked oligosaccharides. prior to recombination, each ... | 1994 | 8289380 |
| sensorineural hearing loss in neurobrucellosis. | a case of neurobrucellosis presenting to the otologist with sensorineural hearing loss (snhl) as the predominant clinical feature is reported. the diagnostic features and treatment options are discussed and the need for prolonged combination treatment to prevent relapse and further deterioration of hearing stressed. snhl in neurobrucellosis has hitherto been reported principally in neurology literature as something of an incidental finding and so escapes the attention of otologists. it is hoped ... | 1993 | 8288975 |
| therapy of experimental murine brucellosis with streptomycin alone and in combination with ciprofloxacin, doxycycline, and rifampin. | the in vivo efficacy of streptomycin (str), doxycycline (dox), rifampin (rif), ciprofloxacin (cip), and their combinations was evaluated for a brucella melitensis experimental infection in a mouse model. animals were infected with 2 x 10(4) to 4 x 10(4) cfu of b. melitensis intraperitoneally on day 0 and were randomized to receive, starting on day 7, str alone at 75, 150, or 300 mg/kg of body weight per day intraperitoneally or dox at 6 mg/kg/day orally, rif at 3 mg/kg/day orally, or cip at 200 ... | 1993 | 8285615 |
| [brucellosis: case report and synopsis of 10 cases (1973-1992) in the chur canton hospital]. | a 33-year-old patient from former yugoslavia presented with low back pain and fever. plain x-rays and myelography were normal, but a technetium-99m scan was suspect for vertebral osteomyelitis. serology and blood cultures were positive for brucella (melitensis). antibiotic therapy with tetracycline and rifampicine rapidly improved the signs and symptoms of the infection. a retrospective analysis of 11 cases of brucellosis treated in the kantonsspital, chur, over the years 1973 to 1992 revealed t ... | 1993 | 8272813 |
| brucellosis of the spine. | the spinal form of brucellosis is still a disabling disease in many countries outside north america and northern and central europe. fifteen consecutive cases of spinal brucellosis diagnosed and treated over a 20-year period were reviewed retrospectively. six patients were farmers, while 10 patients had a history of ingestion of unpasteurized milk or other dairy products. a high index of suspicion is necessary for the diagnosis, since there are no pathognomonic signs or symptoms. radiological as ... | 1993 | 8264880 |
| simultaneous expression of smooth and rough phase properties related to lipopolysaccharide in a strain of brucella melitensis. | brucella strains exhibit either a rough (r) or a smooth (s) colonial phase identifiable by bacteriological methods. this depends on the biosynthesis and translocation to the surface in s but not in r strains, of the o-polysaccharide chain of the lipopolysaccharide (lps) molecule. b. melitensis biovar 1 strain ep exhibited simultaneously both s and r characteristics in relation to colonial morphology, agglutination by monospecific anti-m and anti-r sera, activity of bacteriophages lytic for rough ... | 1993 | 8246253 |
| quinolones in treatment of human brucellosis: comparative trial of ofloxacin-rifampin versus doxycycline-rifampin. | quinolones have been reported to be active against brucella species in vitro. in this prospective randomized study, the efficacy and safety of the combination of ofloxacin plus rifampin were compared with the efficacy and safety of doxycycline plus rifampin, both combinations administered for a 6-week period in treatment of brucellosis. sixty-one patients were enrolled in the study, and 49 had blood or bone marrow cultures positive for brucella melitensis. thirty patients received 200 mg of doxy ... | 1993 | 8239591 |
| evaluation of whole cell and subcellular vaccines against brucella ovis in rams. | five antigen preparations from brucella ovis strain reo 198 were incorporated with the pluronic polymer l-121 and muramyl dipeptide and tested as vaccines against b. ovis infection of rams. the antigenic preparations were: (1) a fraction enriched in outer membrane proteins and rough lipopolysaccharide (hot saline extract, hs); (2) the proteins from hs substantially free of lipopolysaccharide; (3) outer membrane blebs; (4) outer membrane-peptidoglycan complexes extracted with detergent; (5) kille ... | 1993 | 8236802 |
| prevalence of camel brucellosis in libya. | sera of 967 camels of both sexes were tested for antibodies to brucella using the rose bengal plate test, serum agglutination test and the complement fixation test. the prevalence of positive sera was 4.1 per cent. samples collected for cultural examination revealed 9 isolates. five isolates were from milk samples, 3 from aborted foetuses and one from a vaginal swab. all isolates were identified and biotyped as brucella melitensis biovar 1. | 1993 | 8236486 |
| brucella peritonitis. | we report the case of a patient with peritonitis caused by brucella melitensis who also had chronic liver disease. at first the patient was treated for bacterial peritonitis but when a lymphocytic ascites was aspirated antituberculosis chemotherapy was given. however, the serological tests for brucellosis were strongly positive and subsequently b. melitensis was isolated from ascitic fluid. | 1993 | 8228298 |
| anti-brucella activity of ro 23-9424, a dual-action antibacterial. | the in vitro activity of dual-acting antibacterial ro 23-9424 was determined by the agar dilution method against 126 clinical isolates of brucella melitensis. it was compared with fleroxacin, ciprofloxacin and five conventional drugs. mic50 and mic90 for ro 23-9424 were 1.0 and 4.0 mg/l, respectively, as compared with 0.25 and 0.5 mg/l for fleroxacin and ciprofloxacin. one strain which was resistant to other fluoroquinolones, with an mic of > 8.0 mg/l, did not show cross-resistance to ro 23-9424 ... | 1993 | 8222864 |
| successful therapeutic regimens for treating brucella melitensis and brucella abortus infections in cows. | three therapeutic regimens were evaluated in 121 cows naturally infected with brucella melitensis or brucella abortus, using a combination of long-acting oxytetracycline (la-otc), streptomycin (st) and otc-intramammary infusion (imi). cessation of shedding of brucella in udder secretions and absence of brucella in selected tissues were considered criteria for successful treatment. regimen a (tested on 35 cows) consisted of la-otc 25 mg/kg administered intramuscularly (i.m.) every 3 days for 42 d ... | 1993 | 8219341 |
| brucellosis presenting as prolonged fever and hemophagocytosis. | 1993 | 8213671 | |
| antibody response to brucella melitensis outer membrane antigens in naturally infected and rev1 vaccinated sheep. | sera from brucella infected and b. melitensis rev1 vaccinated sheep were analysed by immunoblotting using the cell envelope fraction (cef) of b. melitensis b115. the cef of b. melitensis b115 was analysed using a bank of monoclonal antibodies. the fraction consisted mainly of s-lps like molecules, r-lps and outer membrane proteins (omps) of molecular masses of 10, 16.5, 19, 25-27, 31-34, 36-38, 73 and 89 kda. immunoblot analysis indicates that the antibody response in infected sheep was mainly d ... | 1994 | 8203126 |
| [osteoarthritis caused by brucella melitensis: isolation of the microorganism from the synovial fluid]. | brucellosis is an unusual disease in childhood but the organ manifestations are even more unusual. the authors describe an osteoarthritis caused by brucella melitensis, localized at the tarsal scaphoid in a three years old calabrian child. | 1993 | 8197028 |
| brucellosis: an occupational hazard for medical laboratory personnel. report of five cases. | five cases of laboratory-acquired infection with brucella melitensis are reported. this pathogen is highly contagious when handled in the laboratory. clinicians should alert technologists when brucellosis is suspected so that specimens are handled under the most stringent safety measures. serological surveys and instructions of laboratory workers regarding the clinical diversity of the disease are helpful for early recognition of secondary cases. on the other hand, clinicians should consider bru ... | 1994 | 8181839 |
| urban outbreak of a brucella melitensis infection in an argentine family: clinical and diagnostic aspects. | an outbreak of brucella melitensis in a family was studied. from the fourteen family members who ate unpasteurized goat cheese nine became ill. patients included four females and five males of 8 to 75 years old. in seven of the patients the diagnosis was confirmed by positive blood culture for b. melitensis biovar 1. all the patients were analyzed by standard tube agglutination (sta) and standard tube agglutination with 2-mercaptoethanol (sta-2me) tests at the time of diagnosis. in six of the pa ... | 1994 | 8156050 |
| pleural fluid characteristics in pulmonary brucellosis. | although pulmonary symptoms accompany up to 16% of cases of infection with brucella melitensis, pleural effusion has rarely been reported. a 12 year old girl had brucellosis with pulmonary disease and a pleural effusion. the pleural fluid was clear and straw coloured with 2700 leucocytes/mm3 (93% lymphocytes), a protein level of 48 g/1, and a glucose concentration of 4.1 mmol/l. culture of the pleural fluid grew br melitensis. | 1994 | 8153949 |
| identification and sequence analysis of is6501, an insertion sequence in brucella spp.: relationship between genomic structure and the number of is6501 copies. | an insertion sequence (is) element of brucella ovis, named is6501, was isolated and its complete nucleotide sequence determined. is6501 is 836 bp in length and occurs 20-35 times in the b. ovis genome and 5-15 times in other brucella species. analysis of the junctions at the sites of insertion revealed a small target site duplication of four bases and inverted repeats of 17 bp with one mismatch. is6501 presents significant similarity (53.4%) with is427 identified in agrobacterium tumefaciens, su ... | 1993 | 8126444 |
| identification of an immunoreactive brucella abortus htra stress response protein homolog. | an 11-kb fragment of brucella abortus genomic dna cloned into the bamhi site of puc9 expressed a 60-kda protein in escherichia coli dh5-alpha. antibodies reactive with this 60-kda protein were detected by western blot (immunoblot) analysis in sera from mice, cattle, and goats experimentally infected with b. abortus, in sera from mice experimentally infected with brucella melitensis, and in serum from a dog experimentally infected with brucella canis. similar results were seen with sera obtained ... | 1994 | 8112833 |
| brucella melitensis growth on loewenstein-jensen egg medium from a case of brucella meningitis. | bacteriologic investigation of a cerebrospinal fluid (csf) specimen for mycobacteria on lowenstein-jensen egg medium revealed the presence of brucella organisms. this coincided with a significant antibody titer against brucella spp. this is the first documented report of the ability of b. melitensis organisms to grow on loewenstein-jensen egg medium. | 1993 | 8112041 |
| unusual suppurative complications of brucellosis in children. | three cases with suppurative complications of brucella melitensis infection are presented, demonstrating localized involvement of the brain, bones and lungs, respectively, and the lesions were well defined on radiographic examination. the diagnosis was made on the basis of significant brucella titres and positive blood culture. awareness of such complications and performance of the appropriate serological and bacteriological studies will establish the diagnosis and will also differentiate this d ... | 1993 | 8111184 |
| brucella melitensis rev-1 vaccine as a cause of human brucellosis. | 1993 | 8103891 | |
| esophageal brucellosis: a new location of brucella infection. | the case of a patient with acute brucellosis presenting as lower dysphagia from a paracardial esophageal mass is described. brucella melitensis biotype 3 was isolated from cultured esophageal biopsy material. this is the first case of esophageal brucellosis described in the literature. | 1994 | 8086774 |