Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| intracellular voyeurism: examining the modulation of host cell activities bysalmonella enterica serovar typhimurium. | salmonella spp. can infect host cells by gaining entry through phagocytosis or by inducing host cell membrane ruffling that facilitates bacterial uptake. with its wide host range, salmonella enterica serovar typhimurium has proven to be an important model organism for studying intracellular bacterial pathogenesis. upon entry into host cells, serovar typhimurium typically resides within a membrane-bound compartment termed the salmonella-containing vacuole (scv). from the scv, serovar typhimurium ... | 2005 | 26443522 |
| tricarboxylic acid cycle and glyoxylate bypass. | the tricarboxylic acid (tca) cycle plays two essential roles in metabolism. first, under aerobic conditions the cycle is responsible for the total oxidation of acetyl-coa that is derived mainly from the pyruvate produced by glycolysis. second, tca cycle intermediates are required in the biosynthesis of several amino acids. although the tca cycle has long been considered a "housekeeping" pathway in escherichia coli and salmonella enterica, the pathway is highly regulated at the transcriptional le ... | 2005 | 26443520 |
| imaging techniques for the study of escherichia coli and salmonella infections. | infectious diseases are among the leading causes of mortality worldwide, and numerous bacterial species are included in the vast array of causative agents. this review describes microscopy-based techniques that can be used to study interactions between bacteria and infected host cells, bacterial gene expression in the infected animal, and bacteria-induced cell signaling in eukaryotic cells. as infectious model systems, urinary tract infections caused by uropathogenic escherichia coli (upec) and ... | 2005 | 26443519 |
| hexose/pentose and hexitol/pentitol metabolism. | escherichia coli and salmonella enterica serovar typhimurium exhibit a remarkable versatility in the usage of different sugars as the sole source of carbon and energy, reflecting their ability to make use of the digested meals of mammalia and of the ample offerings in the wild. degradation of sugars starts with their energy-dependent uptake through the cytoplasmic membrane and is carried on further by specific enzymes in the cytoplasm, destined finally for degradation in central metabolic pathwa ... | 2005 | 26443516 |
| two-carbon compounds and fatty acids as carbon sources. | this review concerns the uptake and degradation of those molecules that are wholly or largely converted to acetyl-coenzyme a (coa) in the first stage of metabolism in escherichia coli and salmonella enterica. these include acetate, acetoacetate, butyrate and longer fatty acids in wild type cells plus ethanol and some longer alcohols in certain mutant strains. entering metabolism as acetyl-coa has two important general consequences. first, generation of energy from acetyl-coa requires operation o ... | 2005 | 26443509 |
| transfer rna modification. | transfer rna (trna) from all organisms on this planet contains modified nucleosides, which are derivatives of the four major nucleosides. trna from escherichia coli/salmonella enterica contains 31 different modified nucleosides, which are all, except for one (queuosine[q]), synthesized on an oligonucleotide precursor, which through specific enzymes later matures into trna. the corresponding structural genes for these enzymes are found in mono- and polycistronic operons, the latter of which have ... | 2005 | 26443508 |
| catabolism of amino acids and related compounds. | this review considers the pathways for the degradation of amino acids and a few related compounds (agmatine, putrescine, ornithine, and aminobutyrate), along with their functions and regulation. nitrogen limitation and an acidic environment are two physiological cues that regulate expression of several amino acid catabolic genes. the review considers escherichia coli, salmonella enterica serovar typhimurium, and klebsiella species. the latter is included because the pathways in klebsiella specie ... | 2005 | 26443507 |
| glycerol and methylglyoxal metabolism. | the metabolic connection between glycerol and methylglyoxal (mg) is principally that dhap, which is an intermediate in the aerobic breakdown of glycerol, is also the major precursor of mg, being the substrate for methylglyoxal synthase (mgs). the synthesis of mg is a consequence of unbalanced metabolism related either to a limitation for phosphate or to excessive carbon flux through the pathways that have the capacity to generate significant pools of dhap. cells producing mg produce a poison as ... | 2005 | 26443506 |
| ammonia transport. | this review reviews the ammonium/methylammonium transport (amt) proteins of escherichia coli and salmonella enterica serovar typhimurium. the amt proteins and their homologs, the methylammonium/ammonium permease proteins of saccharomyces cerevisiae, constitute a distinct class of membrane-associated ammonia transporters. members of the amt family are found in archaea, bacteria, fungi, plants, and invertebrate animals. in e. coli and serovar typhimurium, the amt proteins are essential to maintain ... | 2004 | 26443371 |
| biosynthesis of arginine and polyamines. | early investigations on arginine biosynthesis brought to light basic features of metabolic regulation. the most significant advances of the last 10 to 15 years concern the arginine repressor, its structure and mode of action in both e. coli and salmonella typhimurium, the sequence analysis of all arg structural genes in e. coli and salmonella typhimurium, the resulting evolutionary inferences, and the dual regulation of the carab operon. this review provides an overall picture of the pathways, t ... | 2004 | 26443366 |
| regulation of serine, glycine, and one-carbon biosynthesis. | the biosynthesis of serine, glycine, and one-carbon (c1) units constitutes a major metabolic pathway in escherichia coli and salmonella enterica serovar typhimurium. c1 units derived from serine and glycine are used in the synthesis of purines, histidine, thymine, pantothenate, and methionine and in the formylation of the aminoacylated initiator fmet-trnafmet used to start translation in e. coli and serovar typhimurium. the need for serine, glycine, and c1 units in many cellular functions makes ... | 2004 | 26443363 |
| the contribution of aerobic and anaerobic respiration to intestinal colonization and virulence for salmonella typhimurium in the chicken. | the basic mechanism whereby salmonella serovars colonize the chicken intestine remains poorly understood. previous studies have indicated that proton-translocating proteins utilizing oxygen as terminal electron acceptor do not appear to be of major importance in the gut of the newly hatched chicken and consequently they would be even less significant during intestinal colonization of more mature chickens where the complex gut microflora would trap most of the oxygen in the lumen. consequently, a ... | 2015 | 26443064 |
| the aerobic and anaerobic respiratory chain of escherichia coli and salmonella enterica: enzymes and energetics. | escherichia coli contains a versatile respiratory chain that oxidizes 10 different electron donor substrates and transfers the electrons to terminal reductases or oxidases for the reduction of six different electron acceptors. salmonella is able to use two more electron acceptors. the variation is further increased by the presence of isoenzymes for some substrates. a large number of respiratory pathways can be established by combining different electron donors and acceptors. the respiratory dehy ... | 2014 | 26442941 |
| transfer rna modification: presence, synthesis, and function. | transfer rna (trna) from all organisms on this planet contains modified nucleosides, which are derivatives of the four major nucleosides. trna from escherichia coli/salmonella enterica serovar typhimurium contains 33 different modified nucleosides, which are all, except one (queuosine [q]), synthesized on an oligonucleotide precursor, which by specific enzymes later matures into trna. the structural genes for these enzymes are found in mono- and polycistronic operons, the latter of which have a ... | 2014 | 26442937 |
| glycogen: biosynthesis and regulation. | glycogen accumulation occurs in escherichia coli and salmonella enterica serovar typhimurium as well as in many other bacteria. glycogen will be formed when there is an excess of carbon under conditions in which growth is limited because of the lack of a growth nutrient, e.g., a nitrogen source. this review describes the enzymatic reactions involved in glycogen synthesis and the allosteric regulation of the first enzyme, adp-glucose pyrophosphorylase. the properties of the enzymes involved in gl ... | 2014 | 26442935 |
| invasive salmonellosis in humans. | human salmonellosis is generally associated with salmonella enterica from subspecies enterica (subspecies i). acute infections can present in one of four ways: enteric fever, gastroenteritis, bacteremia, or extraintestinal focal infection. as with other infectious diseases, the course and outcome of the infection depend on a variety of factors, including the infecting organism, the inoculating dose, and the immune status and genetic background of the host. for serovarstyphi and paratyphi a there ... | 2012 | 26442824 |
| epidemiology of community-onset bloodstream infections in bouaké, central côte d'ivoire. | bacterial bloodstream infections (bsi) account for considerable morbidity worldwide, but epidemiological data from resource-constrained tropical settings are scarce. we analysed 293 blood cultures from patients presenting to a regional referral hospital in bouaké, central côte d'ivoire, to determine the aetiology of community-onset bsi. the prevalence of bacteraemia was 22.5%, with children being most commonly affected. enterobacteriaceae (predominantly klebsiella pneumoniae and salmonella enter ... | 2015 | 26442153 |
| the novel cis-encoded antisense rna asrc positively regulates the expression of rpoe-rseabc operon and thus enhances the motility of salmonella enterica serovar typhi. | bacterial non-coding rnas are essential in many cellular processes, including response to environmental stress, and virulence. deep sequencing analysis of the salmonella enterica serovar typhi (s. typhi) transcriptome revealed a novel antisense rna transcribed in cis on the strand complementary to rsec, an activator gene of sigma factor rpoe. in this study, expression of this antisense rna was confirmed in s. typhi by northern hybridization. rapid amplification of cdna ends and sequence analysis ... | 2015 | 26441919 |
| attributes of carbapenemase encoding conjugative plasmid pndm-sal from an extensively drug-resistant salmonella enterica serovar senftenberg. | a carbapenem resistant salmonella enterica serovar senftenberg isolate bch 2406 was isolated from a diarrheal child attending an outpatient unit of b.c. roy hospital in kolkata, india. this isolate was positive for the bla ndm-1 in the pcr assay, which was confirmed by amplicon sequencing. except for tetracycline, this isolate was resistant to all the tested antimicrobials. the bla ndm-1 was found to be located on a 146.13-kb mega plasmid pndm-sal, which could be conjugally transferred into esch ... | 2015 | 26441902 |
| model-driven discovery of synergistic inhibitors against e. coli and s. enterica serovar typhimurium targeting a novel synthetic lethal pair, alda and prpc. | mathematical models of biochemical networks form a cornerstone of bacterial systems biology. inconsistencies between simulation output and experimental data point to gaps in knowledge about the fundamental biology of the organism. one such inconsistency centers on the gene alda in escherichia coli: it is essential in a computational model of e. coli metabolism, but experimentally it is not. here, we reconcile this disparity by providing evidence that alda and prpc form a synthetic lethal pair, a ... | 2015 | 26441892 |
| development and comparison of a generic multiple-locus variable-number tandem repeat analysis with pulsed-field gel electrophoresis for typing of salmonella enterica subsp. enterica. | salmonella enterica subsp. enterica causes salmonellosis in humans and animals. serovar-specific multiple-locus variable-number tandem repeat analysis (mlva) is widely used for salmonella surveillance; however, isolates have to be serotyped prior to mlva typing and only the most common serovars can be typed. we developed a mlva scheme for high-discriminatory typing of salmonella. | 2015 | 26440858 |
| temperate phages promote colicin-dependent fitness of salmonella enterica serovar typhimurium. | bacteria employ bacteriocins for interference competition in microbial ecosystems. colicin ib (colib), a pore-forming bacteriocin, confers a significant fitness benefit to salmonella enterica serovar typhimurium (s. tm) in competition against commensal escherichia coli in the gut. colib is released from s. tm into the environment, where it kills susceptible competitors. however, colicin-specific release proteins, as they are known for other colicins, have not been identified in case of colib. th ... | 2016 | 26439675 |
| hospital-based surveillance for infectious etiologies among patients with acute febrile illness in georgia, 2008-2011. | information on the infectious causes of undifferentiated acute febrile illness (afi) in georgia is essential for effective treatment and prevention. in may 2008, a hospital-based afi surveillance was initiated at six hospitals in georgia. patients aged ≥ 4 years with fever ≥ 38°c for ≥ 48 hours were eligible for surveillance. blood culture and serologic testing were conducted for leptospira spp., brucella spp., west nile virus (wnv), crimean-congo hemorrhagic fever virus, coxiella burnetii, tick ... | 2016 | 26438032 |
| [antimicrobial susceptibility of salmonella enterica isolated during the pre-harvest period in swine in colombia]. | to determine the antimicrobial susceptibility of isolates of salmonella enterica isolated from animals and during the pre-slaughter period. | 2015 | 26437705 |
| three phoma spp. synthesised novel silver nanoparticles that possess excellent antimicrobial efficacy. | the authors report extracellular mycosynthesis of silver nanoparticles (agnps) by phoma capsulatum, phoma putaminum and phoma citri. the agnps thus synthesised were characterised by uv-visible spectrophotometer, fourier transform infrared spectroscopy, nanosight lm20 and transmission electron microscopy, which confirmed the synthesis of mostly spherical and polydisperse nanoparticles capped with proteins. the size of agnps was found in the range of 10-80 , 5-80 and 5-90 nm with an average size o ... | 2015 | 26435281 |
| assessment of the effect of a salmonella enterica ser. typhimurium culture supernatant on the single-cell lag time of foodborne pathogens. | the objective of this study was the in vitro evaluation of the effect of a cell-free microbial supernatant, produced by a luxs-positive salmonella enterica ser. typhimurium strain, on the single-cell growth kinetic behavior of two strains of s. enterica (serotypes enteritidis and typhimurium) and a methicillin-resistant staphylococcus aureus strain. the single-cell lag time (λ) of the pathogens was estimated in the absence and presence (20% v/v) of microbial supernatant based on optical density ... | 2015 | 26433459 |
| effect of combined long-term starvation and γ-irradiation on membrane fatty acids and cell surface hydrophobicity of salmonella enterica serovar typhimurium. | this study was carried out to explore the adaptive mechanisms of salmonella enterica serovar typhimurium, in particular the implication of fatty acids (fa) in the remodeling of membrane lipid composition to overcome the combined effects of long-term starvation and γ-irradiation stresses. in addition, cell surface hydrophobicity was also evaluated. the bacterial strains (control and starved) were treated with a nonlethal γ-irradiation dose of 0.5 kgy and sublethal doses of 1 kgy. gas chromatograp ... | 2015 | 26433417 |
| intestinal microbes influence the survival, reproduction and protein profile of trichinella spiralis in vitro. | the interactions between intestinal microbes and parasitic worms play an essential role in the development of the host immune system. however, the effects of gut microbes on trichinella spiralis are unknown. the aim of this work was to explore microbe-induced alterations in the survival and reproduction of t. spiralis in vitro. to further identify the proteins and genes involved in the response of nematodes to microbes, quantitative proteomic analysis of t. spiralis was conducted by itraq-couple ... | 2016 | 26432293 |
| antimicrobial resistance and molecular investigation of h2s-negative salmonella enterica subsp. enterica serovar choleraesuis isolates in china. | salmonella enterica subsp. enterica serovar choleraesuis is a highly invasive pathogen of swine that frequently causes serious outbreaks, in particular in asia, and can also cause severe invasive disease in humans. in this study, 21 s. choleraesuis isolates, detected from 21 patients with diarrhea in china between 2010 and 2011, were found to include 19 h2s-negative s. choleraesuis isolates and two h2s-positive isolates. this is the first report of h2s-negative s. choleraesuis isolated from huma ... | 2015 | 26431037 |
| cross-border outbreak of salmonella enterica ssp. enterica serovar bovismorbificans: multiple approaches for an outbreak investigation in germany and switzerland. | in july 2014, an outbreak of salmonella enterica ssp. enterica serovar bovismorbificans was detected in switzerland. the goal of the outbreak investigation was to rapidly identify and eliminate the contamination source in order to prevent new cases. | 2015 | 26430716 |
| enhancement of immunohistochemical detection of salmonella in tissues of experimentally infected pigs. | salmonella typhimurium is one of the main pathogens compromising porcine and human health as well as food safety, because it is a prevailing source of foodborne infections due to contaminated pork. a prominent problem in the management of this bacteriosis is the number of subclinically infected carrier pigs. as very little is known concerning the mechanisms allowing salmonella to persist in pigs, the objective of this study was to develop an immunohistochemical approach for the detection of salm ... | 2015 | 26428884 |
| evidence supporting vertical transmission of salmonella in dairy cattle. | we set out to investigate whether salmonella enterica could be recovered from various tissues of viable neonatal calves immediately following parturition. eleven samples were aseptically collected from each of 20 calves and consisted of both left and right subiliac and prescapular lymph nodes (ln), mesenteric ln, spleen and liver, as well as intestinal tissue (including luminal contents) from the small intestine, caecum, spiral colon and rectum. in addition, a faecal sample was collected from 19 ... | 2016 | 26419321 |
| comparative growth analysis of capsulated (vi+) and acapsulated (vi-) salmonella typhi isolates in human blood. | salmonella enterica serovar typhi (s. typhi) is a human restricted pathogen. it biosynthesizes a virulence capsular polysaccharide named as vi antigen. s. typhi regulates expression of genes involved in the biosynthesis of vi antigen in response to osmolarity. beside vi-positive isolates, vi-negative (acapsulated) isolates are also pathogenic. however, vi-positive isolates are more prevalent. the present study was planned to investigate comparative growth of vi-positive and vi-negative s. typhi ... | 2015 | 26417360 |
| response of vibrio cholerae to the catecholamine hormones epinephrine and norepinephrine. | in escherichia coli or salmonella enterica, the stress-associated mammalian hormones epinephrine (e) and norepinephrine (ne) trigger a signaling cascade by interacting with the qsec sensor protein. here we show that vibrio cholerae, the causative agent of cholera, exhibits a specific response to e and ne. these catecholates (0.1 mm) enhanced the growth and swimming motility of v. cholerae strain o395 on soft agar in a medium containing calf serum, which simulated the environment within the host. ... | 2015 | 26416829 |
| stall no more at polyproline stretches with the translation elongation factors ef-p and if-5a. | synthesis of polyproline proteins leads to translation arrest. to overcome this ribosome stalling effect, bacteria depend on a specialized translation elongation factor p (ef-p), being orthologous and functionally identical to eukaryotic/archaeal elongation factor e/aif-5a (recently renamed 'ef5'). ef-p binds to the stalled ribosome between the peptidyl-trna binding and trna-exiting sites, and stimulates peptidyl-transferase activity, thus allowing translation to resume. in their active form, bo ... | 2016 | 26416626 |
| sensitive and rapid detection of pathogenic bacteria in small volumes using impedance spectroscopy technique. | we illustrate a novel impedance immunosensor which rapidly and sensitively detects typhoid-causing infectious bacteria salmonella enterica serovar (salmonella typhi) in 10 μl of sample volume. the bacteria are tagged with gold nanoparticles (au nps) via high-affinity antigen-antibody interactions for enhanced signal amplification and selectivity. the cell-particle bioconjugates are then subjected to alternating current (ac) electric fields applied through interdigitated microelectrodes. the immu ... | 2016 | 26414023 |
| comparative genome analysis of inchi2 vim-1 carbapenemase-encoding plasmids of escherichia coli and salmonella enterica isolated from a livestock farm in germany. | carbapenem-resistant enterobacteriaceae are not any more isolated only from human settings, but also from livestock. we reported for the first time the presence of vim-1 carbapenemases in a livestock farm in germany. the vim-1 resistance gene found in these farms was located on inchi2 plasmids. in order to be able to analyse these plasmids in more detail, two different plasmids from a single farm (prh-r27 from salmonella enterica and prh-r178 from escherichia coli) were completely sequenced and ... | 2017 | 26411323 |
| influence of temperature, source, and serotype on biofilm formation of salmonella enterica isolates from pig slaughterhouses. | quantitative assessment of in vitro biofilm formation by 40 salmonella enterica isolates isolated in pig abattoirs from animal and environmental sources (surfaces in contact and not in contact with meat) and classified in eight seroytpes was carried out by using a microtiter plate assay with spectrophotometric reading (optical density at 620 nm). biofilm-forming ability was statistically correlated with the temperature of incubation (22 and 35°c), the source of the isolates, and the antimicrobia ... | 2015 | 26408137 |
| neural network model for survival and growth of salmonella enterica serotype 8,20:-:z6 in ground chicken thigh meat during cold storage: extrapolation to other serotypes. | mathematical models that predict the behavior of human bacterial pathogens in food are valuable tools for assessing and managing this risk to public health. a study was undertaken to develop a model for predicting the behavior of salmonella enterica serotype 8,20:-:z6 in chicken meat during cold storage and to determine how well the model would predict the behavior of other serotypes of salmonella stored under the same conditions. to develop the model, ground chicken thigh meat (0.75 cm(3)) was ... | 2015 | 26408130 |
| survival of salmonella enterica in dried turkey manure and persistence on spinach leaves. | concerns about the microbiological safety of fresh produce have attracted attention in the past three decades due to multiple foodborne outbreaks. animal manure contaminated with enteric pathogens has been identified as an important preharvest pathogen source. this study investigated the survival of salmonella enterica in dust particles of dehydrated turkey manure and how association with manure dust may enhance the survival of salmonellae on leafy greens in the field. the survival of a cocktail ... | 2015 | 26408127 |
| rapid detection of salmonella enterica serovar typhimurium dt104 strains by the polymerase chain reaction. | infection with salmonella enterica is a major public health concern in developed countries, and multidrug-resistant strains have become increasingly prevalent. s. enterica serovar typhimurium dt104 (dt104) strains are prevalent in livestock in japan and include numerous strains of multidrug-resistant s. enterica. epidemiological analysis of these strains is critical for both agriculture and public health; however, diagnostic tests for these strains have yielded inconsistent results. | 2015 | 26408088 |
| complete genome and plasmid sequences of three canadian strains of salmonella enterica subsp. enterica serovar enteritidis belonging to phage types 8, 13, and 13a. | salmonella enterica subsp. enterica serovar enteritidis is a prominent cause of human salmonellosis frequently linked to poultry products. in canada, s. enteritidis phage types 8, 13, and 13a predominate among both clinical and poultry isolates. here, we report the complete genome and plasmid sequences of poultry isolates of these three phage types. | 2015 | 26404595 |
| a highly effective component vaccine against nontyphoidal salmonella enterica infections. | nontyphoidal salmonella enterica (nts) infections are a major burden to global public health, as they lead to diseases ranging from gastroenteritis to systemic infections and there is currently no vaccine available. here, we describe a highly effective component vaccine against s. enterica serovar typhimurium in both gastroenteritis and systemic murine infection models. we devised an approach to generate supernatants of s. enterica serovar typhimurium, an organism that is highly abundant in viru ... | 2015 | 26396246 |
| modulation of cytokine gene expression by selected lactobacillus isolates in the ileum, caecal tonsils and spleen of salmonella-challenged broilers. | probiotics have been used to control salmonella colonization in the chicken intestine. recently, we demonstrated that certain selected lactobacillus isolates were able to reduce salmonella infection in the chicken spleen and liver as well as down-regulated salmonella pathogenicity island 1 virulence gene expression in the chicken caecum. to further understand the mechanisms through which lactobacillus protected chickens from salmonella infection, the present study has investigated the lactobacil ... | 2015 | 26395945 |
| rapidly escalating hepcidin and associated serum iron starvation are features of the acute response to typhoid infection in humans. | iron is a key pathogenic determinant of many infectious diseases. hepcidin, the hormone responsible for governing systemic iron homeostasis, is widely hypothesized to represent a key component of nutritional immunity through regulating the accessibility of iron to invading microorganisms during infection. however, the deployment of hepcidin in human bacterial infections remains poorly characterized. typhoid fever is a globally significant, human-restricted bacterial infection, but understanding ... | 2015 | 26394303 |
| chip-seq analysis of the σe regulon of salmonella enterica serovar typhimurium reveals new genes implicated in heat shock and oxidative stress response. | the alternative sigma factor σe functions to maintain bacterial homeostasis and membrane integrity in response to extracytoplasmic stress by regulating thousands of genes both directly and indirectly. the transcriptional regulatory network governed by σe in salmonella and e. coli has been examined using microarray, however a genome-wide analysis of σe-binding sites in salmonella has not yet been reported. we infected macrophages with salmonella typhimurium over a select time course. using chroma ... | 2015 | 26389830 |
| laboratory, environmental, and epidemiologic investigation and regulatory enforcement actions in response to an outbreak of salmonella bredeney infections linked to peanut butter. | background. in september 2012, the centers for disease control and prevention (cdc), u.s. food and drug administration (fda), and state and local partners investigated an outbreak of salmonella enterica serovar bredeney linked to peanut butter (pb). methods. a case was defined as infection with the outbreak strain of salmonella bredeney between june 1, 2012 and october 31, 2012. food exposure questionnaires were analyzed by the cdc to determine the food vehicle. the fda reviewed production inf ... | 2015 | 26389125 |
| co-expressional conservation in virulence and stress related genes of three gammaproteobacterial species: escherichia coli, salmonella enterica and pseudomonas aeruginosa. | gene co-expression analysis is one of the main aspects of systems biology that uses high-throughput gene expression data. in the present study we applied cross-species co-expressional analysis on a module of biofilm and stress response associated genes. we addressed different kinds of stresses in three most intensively studied members of gammaproteobacteria including escherichia coli k12, pseudomonas aeruginosa pao1 and salmonella enterica for which large sets of gene expression data are availab ... | 2015 | 26387845 |
| poultry body temperature contributes to invasion control through reduced expression of salmonella pathogenicity island 1 genes in salmonella enterica serovars typhimurium and enteritidis. | salmonella enterica serovars typhimurium (s. typhimurium) and enteritidis (s. enteritidis) are foodborne pathogens, and outbreaks are often associated with poultry products. chickens are typically asymptomatic when colonized by these serovars; however, the factors contributing to this observation are uncharacterized. whereas symptomatic mammals have a body temperature between 37°c and 39°c, chickens have a body temperature of 41°c to 42°c. here, in vivo experiments using chicks demonstrated that ... | 2015 | 26386070 |
| global transcriptome and mutagenic analyses of the acid tolerance response of salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium (s. typhimurium) is one of the leading causative agents of food-borne bacterial gastroenteritis. swift invasion through the intestinal tract and successful establishment in systemic organs are associated with the adaptability of s. typhimurium to different stress environments. low-ph stress serves as one of the first lines of defense in mammalian hosts, which s. typhimurium must efficiently overcome to establish an infection. therefore, a better understand ... | 2015 | 26386064 |
| genomic evidence reveals numerous salmonella enterica serovar newport reintroduction events in suwannee watershed irrigation ponds. | our previous work indicated a predominance (56.8%) of salmonella enterica serovar newport among isolates recovered from irrigation ponds used in produce farms over a 2-year period (b. li et al., appl environ microbiol 80:6355-6365, http://dx.doi.org/10.1128/aem.02063-14). this observation provided a valuable set of metrics to explore an underaddressed issue of environmental survival of salmonella by dna microarray. microarray analysis correctly identified all the isolates (n = 53) and differenti ... | 2015 | 26386063 |
| using uvc light-emitting diodes at wavelengths of 266 to 279 nanometers to inactivate foodborne pathogens and pasteurize sliced cheese. | uvc light is a widely used sterilization technology. however, uv lamps have several limitations, including low activity at refrigeration temperatures, a long warm-up time, and risk of mercury exposure. uv-type lamps only emit light at 254 nm, so as an alternative, uv light-emitting diodes (uv-leds) which can produce the desired wavelengths have been developed. in this study, we validated the inactivation efficacy of uv-leds by wavelength and compared the results to those of conventional uv lamps ... | 2015 | 26386061 |
| plant pathogen-induced water-soaking promotes salmonella enterica growth on tomato leaves. | plant pathogen infection is a critical factor for the persistence of salmonella enterica on plants. we investigated the mechanisms responsible for the persistence of s. enterica on diseased tomato plants by using four diverse bacterial spot xanthomonas species that differ in disease severities. xanthomonas euvesicatoria and x. gardneri infection fostered s. enterica growth, while x. perforans infection did not induce growth but supported the persistence of s. enterica. x. vesicatoria-infected le ... | 2015 | 26386057 |
| emergence of vim-1-producing salmonella enterica serovar typhimurium in a paediatric patient. | 2015 | 26384861 | |
| development and evaluation of live attenuated salmonella vaccines in newly hatched duckings. | domestic ducks remain a major source of zoonotic salmonella enterica infections for man worldwide and approaches to protection should include vaccine-mediated immunity. with this in mind we developed several genetically defined mutants in a virulent duck salmonella typhimurium isolate tt-1. from initial tests for virulence in day-old ducks, δrpos, δhila, and δslya mutants retained some virulence so were not studied further. amongst the mutants showing greater attenuation, δssrb, δphopq, δompr, a ... | 2015 | 26382600 |
| in vitro probiotic characterization of lactobacillus strains from fermented radish and their anti-adherence activity against enteric pathogens. | in this study, we evaluated the probiotic properties of lactobacillus plantarum, lactobacillus pentosus, and lactobacillus fermentum strains isolated from fermented radish. all the strains survived the simulated oro-gastrointestinal transit condition and showed significantly higher adherence to caco-2 cells compared with the probiotic strain lactobacillus rhamnosus gg. the strains showed broad-spectrum antimicrobial activity, autoaggregation, and coaggregation capacity with pathogens. furthermor ... | 2015 | 26382558 |
| metal nanoparticle assisted polymerase chain reaction for strain typing of salmonella typhi. | salmonella enterica serotype typhi (s. typhi) is the causative agent of typhoid fever and remains a major health threat in most of the developing countries. the prompt diagnosis of typhoid directly from the patient's blood requires high level of sensitivity and specificity. some of us were the first to report pcr based diagnosis of typhoid. this approach has since then been reported by many scientists using different genomic targets. since the number of bacteria circulating in the blood of a pat ... | 2015 | 26381602 |
| phop, spi1, spi2 and aroa mutants of salmonella enteritidis induce a different immune response in chickens. | poultry is the most frequent reservoir of non-typhoid salmonella enterica for humans. understanding the interactions between chickens and s. enterica is therefore important for vaccine design and subsequent decrease in the incidence of human salmonellosis. in this study we therefore characterized the interactions between chickens and phop, aroa, spi1 and spi2 mutants of s. enteritidis. first we tested the response of hd11 chicken macrophage-like cell line to s. enteritidis infection monitoring t ... | 2015 | 26380970 |
| salmonella isolates in the introduced asian house gecko (hemidactylus frenatus) with emphasis on salmonella weltevreden, in two regions in costa rica. | the asian house gecko hemidactylus frenatus has been widely introduced in costa rica and tends to establish in human settlements. some studies in other invaded countries have suggested that this gecko plays a significant role in the epidemiology of salmonellosis and it is of value to public health. to our knowledge, no studies have examined salmonella from this species in costa rica. therefore, we collected 115 geckos from houses in two costa rican regions. we examined gut contents for salmonell ... | 2015 | 26378974 |
| carbapenemase-producing salmonella enterica serotype kentucky st198, north africa. | 2015 | 26377865 | |
| an orally administered dna vaccine targeting vascular endothelial growth factor receptor-3 inhibits lung carcinoma growth. | lung cancer is the leading cause of mortality and 5-year survival rate is very low worldwide. recent studies show that vascular endothelial growth factor receptor-3 (vegfr-3) signaling pathway contributes to lung cancer progression. so we hypothesize that an oral dna vaccine that targets vegfr-3 carried by attenuated salmonella enterica serovar typhimurium strain sl3261 has impacts on lung cancer progression. in this study, the oral vegfr-3-based vaccine-immunized mice showed appreciable inhibit ... | 2016 | 26376999 |
| septic arthritis in infants younger than 3 months: a retrospective review. | septic arthritis in infants is rare and can be difficult to diagnose. this study reviewed a series of patients younger than 3 months to identify factors that may assist in early diagnosis and treatment. a query of records at a large midwestern pediatric hospital (1994-2010) was performed to identify all patients younger than 3 months at the time of diagnosis. analysis included birth history, joint involvement, physical examination findings, laboratory results, imaging results, method of treatmen ... | 2015 | 26375536 |
| overexpression of salmonella enterica serovar typhi reca gene confers fluoroquinolone resistance in escherichia coli dh5α. | a spontaneous fluoroquinolone-resistant mutant (stm1) was isolated from its parent salmonella enterica serovar typhi (s. typhi) clinical isolate. unlike its parent isolate, this mutant has selective resistance to fluoroquinolones without any change in its sensitivity to various other antibiotics. dna gyrase assays revealed that the fluoroquinolone resistance phenotype of the stm1 mutant did not result from alteration of the fluoroquinolone sensitivity of the dna gyrase isolated from it. to study ... | 2015 | 26375447 |
| salmonella enterica subsp. arizonae meningitis in a neonate exposed to a corn snake. | 2015 | 26367812 | |
| eric-pcr genotyping of field isolates of salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum. | salmonella gallinarum (sg) and salmonella pullorum (sp) have been classified as biovars belonging to salmonella enterica subsp. enterica serovar gallinarum. genetic diversity among isolates of the same biovar can be detected by dna fingerprinting techniques which are useful in epidemiological investigations. in this study, we applied the pcr amplification of enterobacterial repetitive intergenic consensus sequences (eric-pcr) to analyse 45 strains of sg and sp, most of which were isolated from d ... | 2015 | 26365161 |
| factors associated with non-typhoidal salmonella bacteremia versus typhoidal salmonella bacteremia in patients presenting for care in an urban diarrheal disease hospital in bangladesh. | non-typhoidal salmonella (nts) and salmonella enterica serovar typhi bacteremia are the causes of significant morbidity and mortality worldwide. there is a paucity of data regarding nts bacteremia in south asia, a region with a high incidence of typhoidal bacteremia. we sought to determine clinical predictors and outcomes associated with nts bacteremia compared with typhoidal bacteremia. | 2015 | 26361076 |
| prevalence and characterization of monophasic salmonella serovar 1,4,[5],12:i:- of food origin in china. | salmonella enterica subsp. enterica serovar 1,4,[5],12:i:- is a monophasic variant of salmonella typhimurium, which has recently been recognized as an emerging cause of infection worldwide. this bacterium has also ranked among the four most frequent serovars causing human salmonellosis in china. however, there are no reports on its contamination in chinese food. serotyping, polymerase chain reaction, antibiotic resistance, virulotyping, and multilocus sequence typing (mlst) assays were used to i ... | 2015 | 26360603 |
| correction for blondel et al., the type vi secretion system encoded in salmonella pathogenicity island 19 is required for salmonella enterica serotype gallinarum survival within infected macrophages. | 2015 | 26359370 | |
| splenic gene expression profiling in white leghorn layer inoculated with the salmonella enterica serovar enteritidis. | salmonella enterica serovar enteritidis (se) is a foodborne pathogen that can threaten human health through contaminated poultry products. live poultry, chicken eggs and meat are primary sources of human salmonellosis. to understand the genetic resistance of egg-type chickens in response to se inoculation, global gene expression in the spleen of 20-week-old white leghorn was measured using the agilent 4 × 44 k chicken microarray at 7 and 14 days following se inoculation (dpi). results showed tha ... | 2015 | 26358731 |
| draft genome sequence of salmonella enterica subsp. enterica serovar napoli strain sn310, cause of a multischool outbreak in milan, italy, in 2014. | we report the draft genome sequence of salmonella enterica subsp. enterica serovar napoli strain sn310, isolated from a stool sample of an affected pupil during a multischool outbreak in 2014 in milan, italy. this represents the first reported draft genome sequence of the emerging serovar napoli. | 2015 | 26358605 |
| genome sequences of two strains of salmonella enterica serovar enteritidis isolated from shell eggs. | this report presents the complete genome sequences of two salmonella enterica serovar enteritidis strains bearing the pulsed-field gel electrophoresis profile jegx01.0004, which were isolated from the internal contents of eggs. | 2015 | 26358589 |
| correction: genomic comparison of non-typhoidal salmonella enterica serovars typhimurium, enteritidis, heidelberg, hadar and kentucky isolates from broiler chickens. | 2015 | 26352414 | |
| role of host cell-derived amino acids in nutrition of intracellular salmonella enterica. | the facultative intracellular pathogen salmonella enterica resides in a specific membrane-bound compartment termed the salmonella-containing vacuole (scv). despite being segregated from access to metabolites in the host cell cytosol, salmonella is able to efficiently proliferate within the scv. we set out to unravel the nutritional supply of salmonella in the scv with focus on amino acids. we studied the availability of amino acids by the generation of auxotrophic strains for alanine, asparagine ... | 2015 | 26351287 |
| refined live attenuated salmonella enterica serovar typhimurium and enteritidis vaccines mediate homologous and heterologous serogroup protection in mice. | invasive nontyphoidal salmonella (nts) infections constitute a major health problem among infants and toddlers in sub-saharan africa; these infections also occur in infants and the elderly in developed countries. we genetically engineered a salmonella enterica serovar typhimurium strain of multilocus sequence type 313, the predominant genotype circulating in sub-saharan africa. we evaluated the capacities of s. typhimurium and salmonella enterica serovar enteritidis δguaba δclpx live oral vaccin ... | 2015 | 26351285 |
| intra- and inter-species interactions within biofilms of important foodborne bacterial pathogens. | a community-based sessile life style is the normal mode of growth and survival for many bacterial species. under such conditions, cell-to-cell interactions are inevitable and ultimately lead to the establishment of dense, complex and highly structured biofilm populations encapsulated in a self-produced extracellular matrix and capable of coordinated and collective behavior. remarkably, in food processing environments, a variety of different bacteria may attach to surfaces, survive, grow, and for ... | 2015 | 26347727 |
| the extended regulatory networks of sxt/r391 integrative and conjugative elements and inca/c conjugative plasmids. | nowadays, healthcare systems are challenged by a major worldwide drug resistance crisis caused by the massive and rapid dissemination of antibiotic resistance genes and associated emergence of multidrug resistant pathogenic bacteria, in both clinical and environmental settings. conjugation is the main driving force of gene transfer among microorganisms. this mechanism of horizontal gene transfer mediates the translocation of large dna fragments between two bacterial cells in direct contact. inte ... | 2015 | 26347724 |
| the o-antigen negative ∆wbav mutant of salmonella enterica serovar enteritidis shows adaptive resistance to antimicrobial peptides and elicits colitis in streptomycin pretreated mouse model. | salmonella enterica serovar enteritidis, the most common cause of human gastroenteritis, employs several virulence factors including lipopolysaccharide (lps) for infection and establishment of disease inside the host. the lps of s. enterica serovar enteritidis consists of lipid a, core oligosaccharide and o-antigen (oag). the oag consists of repeating units containing different sugars. the sugars of oag are synthesized and assembled by a set of enzymes encoded by genes organized into clusters. p ... | 2015 | 26346801 |
| charge and aggregation pattern govern the interaction of plasticins with lps monolayers mimicking the external leaflet of the outer membrane of gram-negative bacteria. | bacterial resistance to antibiotics has become today a major public health issue. in the development of new anti-infectious therapies, antimicrobial peptides appear as promising candidates. however, their mechanisms of action against bacterial membranes are still poorly understood. we describe for the first time the interaction and penetration of plasticins into lipid monolayers and bilayers modeling the two leaflets of the asymmetrical outer membrane of gram-negative bacteria. the lipid composi ... | 2015 | 26343162 |
| deletion of invasion protein b in salmonella enterica serovar typhimurium influences bacterial invasion and virulence. | salmonella enterica serovar typhimurium (s. typhimurium) has a wide host range and causes infections ranging from severe gastroenteritis to systemic infections in human, as well as causing typhoid-like disease in murine models of infection. s. typhimurium translocates its effector proteins through the salmonella pathogenicity island-i (spi-i)-encoded t3ss-i needle complex. this study focuses on invasion protein b (sipb) of s. typhimurium, which plays an active role in spi-i invasion efficiency. ... | 2015 | 26341924 |
| binding interface between the salmonella σ(s)/rpos subunit of rna polymerase and crl: hints from bacterial species lacking crl. | in many gram-negative bacteria, including salmonella enterica serovar typhimurium (s. typhimurium), the sigma factor rpos/σ(s) accumulates during stationary phase of growth, and associates with the core rna polymerase enzyme (e) to promote transcription initiation of genes involved in general stress resistance and starvation survival. whereas σ factors are usually inactivated upon interaction with anti-σ proteins, σ(s) binding to the crl protein increases σ(s) activity by favouring its associati ... | 2015 | 26338235 |
| contamination of knives and graters by bacterial foodborne pathogens during slicing and grating of produce. | poor hygiene and improper food preparation practices in consumers' homes have previously been demonstrated as contributing to foodborne diseases. to address potential cross-contamination by kitchen utensils in the home, a series of studies was conducted to determine the extent to which the use of a knife or grater on fresh produce would lead to the utensil's contamination with escherichia coli o157:h7 or salmonella enterica. when shredding inoculated carrots (ca. 5.3 log cfu/carrot), all graters ... | 2015 | 26338127 |
| characterization of antimicrobial resistance in salmonella enterica strains isolated from brazilian poultry production. | antimicrobial resistance profiles and presence of resistance determinants and integrons were evaluated in salmonella enterica strains from brazilian poultry. the analysis of 203 isolates showed that those from the poultry environment (88 isolates) were significantly more resistant to antimicrobials than isolates from other sources, particularly those isolated from poultry by-product meal (106 isolates). thirty-seven isolates were resistant to at least three antimicrobial classes. class 1 integro ... | 2015 | 26337044 |
| uncovering what lies beneath a salmonella enterica empyema. | a 67-year-old woman with myelodysplastic syndrome (mds) and transfusional haemosiderosis developed salmonella empyema caused by direct extension from splenic abscesses. she was successfully treated with antibiotics, pleural decortication and splenectomy. she had presented with fever after being treated for presumed pneumonia and parapneumonic effusion 2 months prior. ct scan showed splenic abscesses eroding through the diaphragm causing a left pleural empyema. pleural fluid and spleen bacterial ... | 2015 | 26336186 |
| lipocalin-2 ensures host defense against salmonella typhimurium by controlling macrophage iron homeostasis and immune response. | lipocalin-2 (lcn2) is an innate immune peptide with pleiotropic effects. lcn2 binds iron-laden bacterial siderophores, chemo-attracts neutrophils and has immunomodulatory and apoptosis-regulating effects. in this study, we show that upon infection with salmonella enterica serovar typhimurium, lcn2 promotes iron export from salmonella-infected macrophages, which reduces cellular iron content and enhances the generation of pro-inflammatory cytokines. lcn2 represses il-10 production while augmentin ... | 2015 | 26332507 |
| antibiotic resistance determinants and genetic analysis of salmonella enterica isolated from food in morocco. | antimicrobial-resistant non-typhoidal salmonella (nts) are an important cause of infection in africa, but there is a lack of information on their molecular mechanisms of resistance and epidemiology. this study contributes to fill this gap through the characterization by pulsed-field gel electrophoresis (pfge), multilocus sequence typing (mlst), plasmid profiling and analysis of antibiotic-resistance determinants of 94 salmonella enterica strains isolated from food in morocco. pfge revealed consi ... | 2015 | 26325598 |
| typhoid fever complicated by hemophagocytic lymphohistiocytosis and rhabdomyolysis. | hemophagocytic lymphohistiocytosis (hlh) and rhabdomyolysis are rare complications of typhoid fever from salmonella enterica serovar typhi. herein, we describe the clinical features in a 21-year-old female from india who presented to the intensive care unit with fever, severe pancytopenia, and rhabdomyolysis. | 2015 | 26324725 |
| induction of the sugar-phosphate stress response allows saccharomyces cerevisiae 2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate synthase to function in salmonella enterica. | thiamine pyrophosphate is a required cofactor for all forms of life. the pyrimidine moiety of thiamine, 2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate (hmp-p), is synthesized by different mechanisms in bacteria and plants compared to fungi. in this study, salmonella enterica was used as a host to probe requirements for activity of the yeast hmp-p synthase, thi5p. thi5p synthesizes hmp-p from histidine and pyridoxal-5-phosphate and was reported to use a backbone histidine as the substrate, ... | 2015 | 26324451 |
| effect of alginate coatings with cinnamon bark oil and soybean oil on quality and microbiological safety of cantaloupe. | the quality and microbiological safety of cantaloupes can potentially be improved using antimicrobial coatings that are able to maintain effectiveness throughout storage. the objective of this work was to study the effect of coating mixtures containing sodium alginate and cinnamon bark oil (cbo) on the quality of cantaloupes and the survival of inoculated bacterial pathogens and naturally occurring yeasts and molds during ambient storage at 21 °c. cantaloupes were dipped in mixtures containing 1 ... | 2015 | 26318410 |
| coadministration of cruzipain and gm-csf dnas, a new immunotherapeutic vaccine against trypanosoma cruzi infection. | therapeutic vaccine research and development are especially important in chagas disease considering the characteristics of the chronic infection and the number of people in the americas living with a parasite infection for decades. we have previously reported the efficacy of attenuated salmonella enterica (s) carrying plasmid encoding cruzipain (scz) to protect against trypanosoma cruzi infection. in the present work we investigated whether cz dna vaccine immunotherapy could be effective in cont ... | 2016 | 26312947 |
| genomic variability of serial human isolates of salmonella enterica serovar typhimurium associated with prolonged carriage. | salmonella enterica serovar typhimurium is an important foodborne human pathogen that often causes self-limiting but severe gastroenteritis. prolonged excretion of s. typhimurium after the infection can lead to secondary transmissions. however, little is known about within-host genomic variation in bacteria associated with asymptomatic shedding. genomes of 35 longitudinal isolates of s. typhimurium recovered from 11 patients (children and adults) with culture-confirmed gastroenteritis were seque ... | 2015 | 26311853 |
| [structure and function of the bacterial flagellar type iii protein export system in salmonella ]. | the bacterial flagellum is a filamentous organelle that propels the bacterial cell body in liquid media. for construction of the bacterial flagellum beyond the cytoplasmic membrane, flagellar component proteins are transported by its specific protein export apparatus from the cytoplasm to the distal end of the growing flagellar structure. the flagellar export apparatus consists of a transmembrane export gate complex and a cytoplasmic atpase ring complex. flagellar substrate-specific chaperones b ... | 2015 | 26310179 |
| small rna-based feedforward loop with and-gate logic regulates extrachromosomal dna transfer in salmonella. | horizontal gene transfer via plasmid conjugation is a major driving force in microbial evolution but constitutes a complex process that requires synchronization with the physiological state of the host bacteria. although several host transcription factors are known to regulate plasmid-borne transfer genes, rna-based regulatory circuits for host-plasmid communication remain unknown. we describe a posttranscriptional mechanism whereby the hfq-dependent small rna, rpra, inhibits transfer of pslt, t ... | 2015 | 26307765 |
| genotypic diversity, pathogenic potential and the resistance profile of salmonella typhimurium strains isolated from humans and food from 1983 to 2013 in brazil. | salmonella enterica subsp. enterica serovar typhimurium is one of the leading serovars that causes salmonellosis worldwide. however, few studies have molecularly characterized s. typhimurium strains in brazil. in this study, we genotyped 92 s. typhimurium strains isolated from humans (43) and food (49) between 1983 and 2013 in brazil using pfge, multiple-locus variable number of tandem repeats analysis (mlva) and enterobacterial repetitive intergenic consensus pcr (eric-pcr). moreover, we assess ... | 2015 | 26307078 |
| microbiological characterization of salmonella enterica serotype paratyphi, south africa, 2003-2014. | 2015 | 26306409 | |
| comparison of two culture techniques used to detect environmental contamination with salmonella enterica in a large-animal hospital. | salmonellosis is a common healthcare-associated infection in large-animal hospitals, and surveillance for salmonella is an integral part of comprehensive infection control programmes in populations at risk. the present study compares the effectiveness of two culture techniques for recovery of salmonella from environmental samples obtained in a large-animal referral veterinary hospital during a salmonella outbreak. environmental samples were collected using household cleaning cloths that were inc ... | 2015 | 26304139 |
| acquisition of the lac operon by salmonella enterica. | classical bacteriological characteristics of salmonella enterica indicate that the members of this species are unable to utilize lactose as a carbon source. however, lactose-fermenting (lac+) strains of several salmonella serovars have been isolated from different foodborne outbreaks as well as different geographical regions worldwide. in the present study, we sequenced the genomes of 13 lac + s. enterica isolates and characterized the lac region, comparing it to the lac region in other enteric ... | 2015 | 26303940 |
| broad spectrum antimicrobial compounds from the bacterium exiguobacterium mexicanum mssrfs9. | clinical bacterial pathogens front a major challenge for the clinical researchers and physicians. in particular microbial pathogens like escherichia coli, shigella flexneri, klebsiella pneumonia and salmonella enterica are apparelled with systemic machineries to bring down the human immune system as well as proliferate dramatically in a short period which in turn cause a pronounced ailment to the human health. in vitro evaluation of four purified compounds isolated from rhizosphere bacterium exi ... | 2015 | 26302848 |
| the two-component system cpxr/a represses the expression of salmonella virulence genes by affecting the stability of the transcriptional regulator hild. | salmonella enterica can cause intestinal or systemic infections in humans and animals mainly by the presence of pathogenicity islands spi-1 and spi-2, containing 39 and 44 genes, respectively. the arac-like regulator hild positively controls the expression of the spi-1 genes, as well as many other salmonella virulence genes including those located in spi-2. a previous report indicates that the two-component system cpxr/a regulates the spi-1 genes: the absence of the sensor kinase cpxa, but not t ... | 2015 | 26300871 |
| salmonella disrupts host endocytic trafficking by sopd2-mediated inhibition of rab7. | intracellular bacterial pathogens of a diverse nature share the ability to evade host immunity by impairing trafficking of endocytic cargo to lysosomes for degradation, a process that is poorly understood. here, we show that the salmonella enterica type 3 secreted effector sopd2 mediates this process by binding the host regulatory gtpase rab7 and inhibiting its nucleotide exchange. consequently, this limits rab7 interaction with its dynein- and kinesin-binding effectors rilp and fyco1 and thereb ... | 2015 | 26299973 |
| genetic and resistance phenotypic subtyping of salmonella saintpaul isolates from various food sources and humans: phylogenetic concordance in combinatory analyses. | bacterial pathogen subtyping for public health traceback of foodborne outbreaks has increasingly produced a number of disparate molecular techniques of varying resolution. here, we bridge the molecular divide across three methodologies, transform data types for cross-comparison, and test phylogenetic concordance. single nucleotide polymorphism (snp) discovery was combined with pulsed-field gel electrophoresis (pfge) and antimicrobial susceptibility profiles for identifying and differentiating 18 ... | 2015 | 26299886 |