Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| influence of maternal antibody on infection with virulent or attenuated marek's disease herpesvirus. | 1972 | 4335611 | |
| production of circulating and cell-bound interferon in chickens by type 1 and type 2 plaque-producing agents of the cal-1 strain of marek's disease herpesvirus and herpesvirus of turkeys. | 1972 | 4335399 | |
| experiments on the mode of spreading of marek's disease virus. | 1971 | 4334325 | |
| the temporal relationship between vaccination with the herpesvirus of turkeys and challenge with virulent marek's disease virus. | 1971 | 4334075 | |
| influence of natural exposure on response to subsequent challenge with virulent marek's disease virus. | 1971 | 4334074 | |
| herpesviral isolates related to marek's disease virus from chicken and turkey kidney cell cultures. | 1971 | 4334004 | |
| structural proteins of marek's disease virus. | 1972 | 4333738 | |
| marek's disease herpesvirus: growth and detection in vivo and in vitro. | 1972 | 4333637 | |
| detection of virus-associated antigen on membranes of cells productively infected with marek's disease herpesvirus. | 1972 | 4333493 | |
| immunisation against newcastle disease in the presence of marek's disease virus. | 1971 | 4332079 | |
| interactions between marek's disease herpesvirus and avian leucosis virus in tissue culture. | 1971 | 4331996 | |
| marek's disease herpesvirus in peripheral nerve lesions. | 1970 | 4331641 | |
| an improved in vitro assay for cell-free marek's disease virus. brief report. | 1971 | 4330260 | |
| antibodies for marek's disease virus in sera from domestic chickens and wild fowl in kenya. | 1971 | 4329207 | |
| large-scale production of marek's disease virus. | 1971 | 4329206 | |
| some characteristics of cell-free preparations of marek's disease virus. | 1971 | 4329205 | |
| influence of bursa of fabricius on infection and pathological response of chickens exposed to marek's disease herpesvirus. | 1971 | 4329202 | |
| the effect of marek's disease virus on the erythrocyte content of chicken blood. | 1971 | 4328662 | |
| further studies on the replication of marek's disease virus in the chicken and in cell culture. | 1971 | 4328192 | |
| effect of vaccination with herpesvirus of turkeys (hvt) on horizontal spread of marek's disease herpesvirus. | 1971 | 4325826 | |
| evidence against embryo transmission of marek's disease virus. | 1970 | 4323098 | |
| marek's disease herpesvirus: comparison of foci (macro) in infected duck embryo fibroblasts under agar medium with foci (micro) in chicken cells. | 1970 | 4323091 | |
| specificity of the humoral and pathological response to marek's disease virus. | 1970 | 4322705 | |
| ultrastructural studies of a herpesvirus of turkeys antigenically related to marek's disease virus. | 1971 | 4322626 | |
| lyophilization of cell-free marek's disease herpesvirus and a herpesvirus from turkeys. | cell extracts of the jm and ga strains of marek's disease herpesvirus and the fc 126 strain of turkey herpesvirus were lyophilized with various stabilizers. much higher virus titers were obtained with stabilizer than without stabilizer. titers increased even further in the case of the marek's disease virus strains by the addition of a chelating agent, disodium ethylenediaminetetraacetate. | 1970 | 4320863 |
| the influence of ultraviolet-inactivated sendai virus on marek's disease virus infection in tissue culture. | 1970 | 4320685 | |
| studies on the epidemiology of marek's disease herpesvirus in broiler flocks. | 1970 | 4316766 | |
| isolation from turkeys of a cell-associated herpesvirus antigenically related to marek's disease virus. | 1970 | 4314928 | |
| marek's disease herpesvirus: in vivo and in vitro infection of kidney cells of different genetic strains of chickens. | 1969 | 4311861 | |
| localization of viral antigen in chickens infected with marek's disease herpesvirus. | 1969 | 4310100 | |
| comparison of antigenic glycoproteins and glycoprotein receptors of concanavalin a isolated from duck embryo cells infected with marek's disease virus and a herpes virus of turkeys (strain fc126). | 1974 | 4214897 | |
| [comparison of the effectiveness of marek's disease virus isolation from lymphocytes and spleen cells in chickens]. | 1974 | 4210142 | |
| detecting marek's disease herpesvirus: evaluation of biological systems. | 1973 | 4197288 | |
| cell-free transmission and in vivo replication of marek's disease virus. | marek's disease virus recovered from the feather follicle of infected chickens was found to be infectious for chickens in cell-free preparations. the virus replicated in epithelial cells of the germinative layer of the feather follicle epidermis, producing both intranuclear and round or diffuse cytoplasmic inclusion bodies in the infected cells. it was found at this site 2 weeks postinoculation and prior to the development of tumor or other gross lesions. in the nucleus, many naked and a few env ... | 1970 | 4191324 |
| immunoglobulin response in experimental infection with cell-free and cell-associated marek's disease virus. | 1973 | 4123370 | |
| serological relationships among herpesviruses: cross-reaction between marek's disease virus and pseudorabies virus as detected by immunogluorescence. | marek's disease virus (mdv) and pseudorabies virus (prv) cross-reacted by fluorescent antibody test but not by gel diffusion, virus neutralization, or cross-protection tests. herpesvirus of turkeys cross-reacted with mdv but not with prv. other herpesviruses tested did not have cross-reactions. | 1972 | 4117951 |
| responses of cell cultures from various avian species to marek's disease virus and herpesvirus of turkeys. | 1971 | 4107762 | |
| size and composition of marek's disease virus deoxyribonucleic acid. | deoxyribonucleic acid (dna) extracted from purified nucleocapsids of marek's disease herpesvirus (mdv) was cosedimented with t4 and with herpes simplex virus (hsv) dna in neutral sucrose density gradients and with t4 dna in alkaline sucrose density gradients. these experiments indicated that the intact mdv dna had a sedimentation constant of 56s corresponding to a molecular weight of 1.2 x 10(8) daltons. in the alkaline gradients, the largest and most prominent band contains a dna sedimenting at ... | 1971 | 4101222 |
| altered cholesteryl ester cycle is associated with lipid accumulation in herpesvirus-infected arterial smooth muscle cells. | we describe herein the effects of marek's disease herpesvirus (mdv) on cholesterol and cholesteryl ester metabolism in cultured chicken arterial smooth muscle cells. infection of arterial smooth muscle cells from specific pathogen-free chickens with mdv, but not a virus control, herpesvirus of turkeys led to a 7-10-fold increase in the accumulation of free and esterified cholesterol and a 2-fold increase in phospholipids. the cellular lipid changes observed in the mdv-infected arterial smooth mu ... | 1985 | 3997816 |
| variation in histology and growth characteristics of transplantable marek's disease lymphomas. | a comparative study was made of the histology and growth characteristics of three different marek's disease virus-induced transplantable lymphomas. these lymphomas were developed previously in related inbred chicken lines g-b1 and g-b2. the ug1 lymphoma was developed by serial i.m. passage in g-b1 chickens, and the ug2 and ug4 lymphomas were developed similarly in g-b2 chickens. while all three lymphomas grow progressively and cause rapid death in syngeneic hosts, differences in pathogenicity ex ... | 1985 | 3978638 |
| partial protection against marek's disease in chickens immunized with glycoproteins gb purified from turkey-herpesvirus-infected cells by affinity chromatography coupled with monoclonal antibodies. | glycoproteins gb of marek's disease virus (mdv) and herpesvirus of turkeys (hvt) related to virus neutralization were purified from hvt-infected cells by affinity chromatography. immunization of chickens with purified glycoproteins gb resulted in partial protection against md. neutralizing antibodies were detected in chickens immunized with hvt-gb. | 1985 | 3896223 |
| acid alpha naphthyl acetate esterase reacting lymphocytes in the peripheral blood of chickens challenged with marek's disease after vaccination with three different vaccines. | the average percentage of acid alpha naphthyl acetate esterase reacting lymphocytes (aparl) was enumerated in the peripheral blood of chickens challenged with marek's disease after vaccination with either turkey herpesvirus (hvt), inactivated marek's disease virus (imdv) or a mixture of the two (bivalent vaccine). a gradual increase in aparl value was noticed in the vaccinated chickens from day 7 to 70 after challenge with a virulent marek's disease virus. the increase was consistent and signifi ... | 1987 | 3628992 |
| expression of a putative tumor-associated surface antigen on normal versus marek's disease virus-transformed lymphocytes. | various avian tumor cell lines and normal spleen cells from 3 genetic strains of specific-pathogen-free (spf) chickens were examined for expression of marek's disease (md) tumor-associated surface antigen (matsa). two anti-matsa monoclonal antibodies (rph 6 and eb 29) and a rabbit anti-matsa antiserum were used in indirect fluorescent antibody tests, and cells were examined by fluorescence microscopy and with a fluorescence-activated cell sorter (facs). less than 5% matsa-positive cells were obs ... | 1987 | 3479647 |
| marek's disease virus-induced transient paralysis in chickens: electron microscopic lesions. | a study was made to determine the causative lesion of marek's disease virus (mdv)-induced transient paralysis (tp) in chickens by comparing the ultrastructure of brain tissue from mdv-infected genetically susceptible and resistant birds. there were numerous intramyelinic vacuoles in the brains of tp-affected birds. many of these vacuoles contained particulate material compatible with precipitated protein from edema. astrocyte processes often were distended with similar particulate material. most ... | 1988 | 3376763 |
| influence of the major histocompatibility complex on tumor regression and immunity in chickens. | a number of studies show that major histocompatibility complex (mhc) genes control host immune responses to viral-induced chicken tumors. the mhc gene-controlled responses to malignant neoplasms caused by rous sarcoma virus, lymphoid leukosis virus and marek's disease virus are reviewed. genes that determine regression of rous sarcomas and resistance to development of lethal marek's disease lymphomas appear to map within the b-f region of the mhc. in some cases, genetic complementation of both m ... | 1987 | 3306646 |
| antigen a gene of marek's disease virus (mdv) detects two mrna species (3.7 and 2.1 kb) in mdv- and hvt-infected cells. | the gene of glycoprotein a of marek's disease virus (mdv) detected to mrna species (3.7 and 2.1 kb) by northern blot hybridization. these two mrna species were also detected in rna extracted from herpesvirus of turkeys (hvt)-infected chick embryo fibroblasts (cef) and from the msb-1 cell line. | 1988 | 3245131 |
| marek's disease virus-induced transient paralysis in chickens: alterations in brain density. | the presence of brain edema and its distribution in marek's disease virus (mdv)-induced transient paralysis (tp) was determined in genetically resistant and susceptible inbred white leghorn chickens. density measurements on samples from the cerebellum, cerebral cortex, optic chiasm, and optic lobe were determined on continuous density percoll gradients. at 9 days post inoculation of mdv, the cerebellums from the susceptible line of chickens were significantly less dense than cerebellums from the ... | 1988 | 3213432 |
| experimental studies on marek's disease in japanese quail (coturnix coturnix japonica). | day-old quails experimentally infected with marek's disease (md) virus of quail origin developed lymphoid tumors. the severity of the disease increased considerably with serial passage. tumor transplants could be made with cells derived from gross tumors in skeletal muscles, spleen cells, and blood from md-affected quails. after five to six serial transplants, the tumor could not be transplanted further. marek's disease tumor-associated surface antigen (matsa) was demonstrated in lymphoid cells ... | 1987 | 3039961 |
| effect of acyclovir on the replication of turkey herpesvirus and marek's disease virus. | the toxicity of acyclovir for chick embryo fibroblasts and its effect on the replication of turkey herpesvirus (strain fc 126) and marek's disease virus (strain hprs 16) multiplied on fibroblast culture was studied. the influence of using acyclovir on the development of the tumour process in birds infected with a virulent marek's disease virus was also determined. acyclovir used in doses below 12.5 micrograms ml-1 proved to be nontoxic for chick embryo fibroblast culture. it inhibited in vitro r ... | 1987 | 3039621 |
| immunization of chickens against marek's disease with cell-free supernatant from the jmv-1 lymphoblastoid cell line. | 1987 | 3037562 | |
| identification of the gene encoding marek's disease herpesvirus a antigen. | the gene encoding the glycoprotein marek's disease herpesvirus a antigen (mdhv-a) precursor polypeptide pr47 was delineated by using northern blot (rna blot) analysis and hybrid selection of its mrna with cloned mdhv dna, cell-free translation of the mrna, and immunoprecipitation of the polypeptide. the resulting piece of dna with strongly positive hybrid selection results was a 2.2-kilobase-pair (kbp) pvuii-ecori restriction fragment localized to the center of the 18.3-kbp mdhv bamhi b fragment ... | 1987 | 3037120 |
| efficacy of a bivalent vaccine against marek's disease. | a bivalent vaccine was prepared by combining inactivated marek's disease virus and turkey herpesvirus. the efficacy of this vaccine, compared to turkey herpesvirus and inactivated marek's disease virus separately, was studied in unsexed white leghorn chicks which were vaccinated at one day old and then challenged at 21 days old with fowl blood infected with virulent marek's disease virus. the bivalent vaccine appreciably delayed mortality resulting from marek's disease and elicited the highest p ... | 1987 | 3035660 |
| cultivation of avian rotaviruses in chicken lymphocytes and lymphoblastoid cell lines. | avian rotavirus isolates were used to infect normal chicken spleen cells, lymphoblastoid t cell lines transformed by marek's disease virus, an avian leukosis virus-transformed b cell line, and a reticuloendotheliosis virus-transformed line, which is a pre-b, pre-t cell line. all five isolates tested were able to infect spleen cells and the three types of lymphoblastoid cell lines, suggesting that avian rotaviruses can infect both b and t cells. splenic lymphocytes were considerably less suscepti ... | 1987 | 3034208 |
| analysis of marek's disease virus serotype 1-specific phosphorylated polypeptides in virus-infected cells and marek's disease lymphoblastoid cells. | by use of monoclonal antibodies, a virus-specific cytoplasmic antigen related to phosphorylated polypeptides specific to serotype 1 of marek's disease virus (mdv)-related viruses (mdv1) has been identified in all md tumour cell lines examined, as well as in infected cells and in tumour lesions of chickens with md. at least two phosphorylated polypeptides with mol. wt. 39,000 (39k) to 36k and 24k (pp39/36 and pp24, respectively) were identified in the md tumour cell line h10 cultured at 33 degree ... | 1987 | 3033141 |
| establishment and characterization of a t-lymphoblastoid cell line mdcc-mtb1 derived from chick lymphocytes infected in vitro with marek's disease virus serotype 1. | continuously proliferating t-lymphoblastoid cells, named mdcc-mtb1, were obtained by infection of chick embryo lymphocytes with marek's disease virus serotype i (mdv1) in culture and subsequent cultivation in the presence of human interleukin 2 (il-2). the mtb1 cells have now been growing well for at least 4 months with a doubling time of about 10 hr, irrespective of the presence of il-2. the mtb1 cells show lymphoblastoid morphology, and carry t-lymphocyte marker surface antigens and a karyotyp ... | 1987 | 3030943 |
| restriction enzyme map of herpesvirus of turkey dna and its collinear relationship with marek's disease virus dna. | the genome of herpesvirus of turkey (hvt) was shown to consist of long and short unique regions flanked by inverted repeats (j. cebrian, kaschka-dietrich, c., berthelot, n., and sheldrick, p., 1982, proc. natl. acad. sci. usa 79, 555-558). in this paper we report the construction of the linkage map of hvt dna for bamhi, hindiii, and psti restriction endonucleases. the maps were constructed by hybridization of 19 cloned bamhi fragments of hvt dna to electrophoretically separated digests of genomi ... | 1987 | 3029976 |
| preparation of monoclonal antibodies against marek's disease virus and herpesvirus of turkeys. | 1986 | 3029494 | |
| an improved elisa method, using a streptavidin-biotin complex, for detecting marek's disease virus antigens in feather-tips of infected chickens. | to improve sensitivity in the detection of marek's disease virus (mdv) antigens in extracts of feather tips from infected chickens, we added a preformed streptavidin-biotin complex to the standard enzyme-linked immunosorbent assay (elisa). rabbit anti-chicken igg-alkaline phosphatase that is used in the standard elisa as the conjugate was replaced by a biotinylated rabbit anti-chicken igg plus the streptavidin-biotin peroxidase complex (abc) system. the abc-elisa system was correlated to the sta ... | 1986 | 3025239 |
| [the effect of short-term isolation of vaccinated chicks on the incidence of marek's disease]. | in chickens immunized by normal and tenfold doses of marvak vaccine (100 and 1,000 pfu) we investigated protective effects against the natural contact infection of chickens that were exposed to the infection immediately after vaccination, or at the intervals of 48 and 72 hours, and 7 and 14 days. studying the elimination of marek's disease virus by feather follicles, the health condition of chickens, tumor frequency and mortality rate we demonstrated that neither of these doses could protect the ... | 1986 | 3020763 |
| precipitating antibody development in chicks experimentally infected with herpes virus of turkey and marek's disease virus. | 1986 | 3018071 | |
| [immunological aspects of marek's disease in poultry]. | 1986 | 3017267 | |
| isolation of very virulent pathotypes of marek's disease virus from vaccinated chickens in europe. | two very virulent strains of marek's disease virus were isolated from two separate farms in northern italy that had experienced outbreaks of marek's disease in vaccinated flocks. the isolates were similar to very virulent strains reported in the usa in terms of their enhanced pathogenicity, both for chickens vaccinated with the herpesvirus of turkeys, and for genetically resistant chickens. this first description of very virulent strains of marek's disease virus from outside the usa suggests tha ... | 1986 | 3016970 |
| molecular characterization of marek's disease herpesvirus b antigen. | the marek's disease herpesvirus (mdhv) b antigen (mdhv-b) was identified and molecularly characterized as a set of three glycoproteins of 100,000, 60,000, and 49,000 apparent molecular weight (gp100, gp60, and gp49, respectively) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) after immunoprecipitation from [35s]methionine-labeled infected cells by specific rabbit antiserum directed against mdhv-b (r alpha b), as previously determined by immunodiffusion. further identific ... | 1986 | 3016305 |
| detection of marek's disease virus antigens and dna in feathers from infected chickens. | two novel tests, enzyme-linked immunosorbent assay (elisa) and dot-blot hybridization, were developed to detect and quantify the antigens and dna of marek's disease virus (mdv) in feather tips from infected chickens. in both methods, buffered extracts of the feathers served as the same test material. the elisa technique was compared to the conventional agar-gel precipitation (agp) test, using the same convalescent serum from a mdv-infected bird. of 86 feather samples tested, 34 were negative by ... | 1986 | 3016019 |
| use of lectins to detect and differentiate subtypes of marek's disease virus and turkey herpesvirus glycoproteins in tissue culture. | biotinylated lectins in conjunction with an avidin-biotin-peroxidase complex were used for the first time to reveal glycoproteins in chicken and duck embryo fibroblasts infected with three prototype members of the avian herpesvirus group, marek's disease virus serotypes 1 and 2 and turkey herpesvirus. by using a panel of 10 lectins, a pattern of reactivity emerged which was both group- and type-specific. morphological details of the lectin-stained cells include cytoplasmic granulation, capping a ... | 1986 | 3016013 |
| protective efficacy of marek's disease virus (mdv) cvi-988 cef65 clone c against challenge infection with three very virulent mdv strains. | comparative 50% protective dose (pd50) assays were performed using a plaque-purified preparation of marek's disease virus (mdv) strain cvi-988 at the 65th chicken embryo fibroblast (cef) passage level (mdv cvi-988 cef65 clone c) and three commercial md vaccines: herpesvirus of turkeys (hvt) fc126, mdv cvi-988 cef35, and a bivalent vaccine composed of hvt fc126 and mdv sb-1. in addition, comparative pd50 assays were performed in groups of chickens with maternal antibody to each of the three vacci ... | 1986 | 3015113 |
| pathogenicity studies with plaque-purified preparations of marek's disease virus strain cvi-988. | the pathogenicity of marek's disease (md) strain cvi-988 vaccine, eight plaque-purified preparations originating from this strain, and the vaccine hvt fc126 (based on herpesvirus of turkeys) was determined by intramuscular administration of high virus doses to day-old specific-pathogen-free rhode island red (rir) chickens, which are extremely md-susceptible. paralysis and neuritis were observed in 88% of rir chickens inoculated with mdv cvi-988 at the cell-passage level of the commercial vaccine ... | 1986 | 3015112 |
| [enhanced pathenogicity of chicken anemia agent (caa) in dual infections with marek's disease virus (mdv), infectious bursal disease virus (ibdv) or reticuloendotheliosis virus (rev)]. | 1986 | 3014789 | |
| [dynamics of passive humoral immunity transfer against marek's disease in the progeny of the grandparent breeding stock of broilers]. | in the progeny of the grandparents of broiler type hybro, imported by the dutch firm euribrid, neonatal humoral immunity was studied during the whole laying period, i.e. in laying hens at the age of 26-52 weeks. precipitation test was used to examine 292 samples of sera from the parent chickens of sire stock ab and 299 samples of sera from the chickens of dam stock cd, for the presence of maternal antibodies to marek's disease virus. the number of positive samples decreased with the growing age ... | 1986 | 3014704 |
| a comparison of the biological properties of type 2 plaque-producing agent derived from the cal-1 strain of marek's disease virus with other related viruses. | the serological and biological properties of the type 2 plaque-producing agent (ppa) derived from the cal-1 strain of marek's disease virus (mdv) were compared with those of reference strains of the three serotypes of mdv and herpesvirus of turkeys (hvt) groups; namely jm, hprs-24 strains of mdv and the fc-126 strain of hvt for serotype 1, 2, and 3, respectively. by agar gel precipitation (agp), indirect fluorescent antibody and virus neutralization tests, type 2 ppa was related but not identica ... | 1986 | 3013131 |
| chromosomal sites for marek's disease virus dna in two chicken lymphoblastoid cell lines mdcc-msb1 and mdcc-rp1. | in situ hybridization of viral dna fragments to metaphase chromosomes of two chicken t-lymphoblastoid cell lines (mdcc) demonstrated that marek's disease virus dna was located in the short arm of chromosome 2 of mdcc-msb1 and mdcc-rp1 cells and in the short arm of chromosome 4 of mdcc-msb1 cells. the mdv dna sequences in these chromosomes are not in the vicinity of oncogenes or integrated retroviruses that have been previously identified in chicken chromosomes. | 1986 | 3012870 |
| identification with monoclonal antibodies of virus-specific dna-binding proteins in the nuclei of cells infected with three serotypes of marek's disease virus-related viruses. | two groups of virus-specific polypeptides were identified in the nuclei of infected cells by cross-reacting monoclonal antibodies with three serotypes of marek's disease virus. of these, a 135,000-molecular-weight polypeptide common to all three serotypes was found to bind to both double-stranded and single-stranded dnas. | 1986 | 3012112 |
| herpesvirus infection prevents activation of cytoplasmic cholesteryl esterase in arterial smooth muscle cells. | herpesvirus infection has been shown to alter the cholesteryl ester cycle in avian arterial smooth muscle cells, resulting in cytoplasmic cholesteryl ester accumulation (hajjar, d. p., falcone, d. j., fabricant, c. g., and fabricant, j. (1985) j. biol. chem. 260, 6124-6128). in this study, we attempted to define some of the regulatory mechanisms associated with the control of cytoplasmic cholesteryl esterase in marek's disease herpesvirus (mdv)-infected cells. we found that cholesteryl esterase ... | 1986 | 3011790 |
| amplification of a tandem direct repeat within inverted repeats of marek's disease virus dna during serial in vitro passage. | dna of the oncogenic strain bc-1 of marek's disease virus contains three units of tandem direct repeats with 132 base pairs in the terminal repeat and internal repeat, respectively, of the long region of the marek's disease virus genome, whereas the attenuated, nononcogenic viral dna contains multiple units of the tandem direct repeats. | 1986 | 3009876 |
| tests of association of immunoglobulin allotype genes and viral oncogenesis in chickens. | chickens from regional poultry research laboratory (rprl) inbred line 6(3) are resistant to virally-induced marek's disease (md) and lymphoid leukosis (ll) and are relatively strong regressors of virally-induced rous sarcomas. in contrast, rprl line 100 chickens are highly susceptible to md and ll and are weaker regressors of rous sarcomas than line 6(3). rprl lines 100 and 6(3) differ for alleles at the igg-1 (g-1) allotype locus, but have identical igm-1 (m-1) allotype alleles. to test the pos ... | 1986 | 3009316 |
| [prevention of marek's disease by various vaccination viruses following test infection at various ages, study of pd50]. | 1986 | 3009131 | |
| [effects of blood group markers (b allele), immunoglobulins and leukosis infections on the development of marek's disease]. | 1986 | 3009129 | |
| embryo vaccination with infectious bursal disease virus alone or in combination with marek's disease vaccine. | studies with specific-pathogen-free chickens revealed that chicks hatching from eggs inoculated at the 18th day of embryonation with infectious bursal disease (ibd) vaccine viruses of low virulence (isolates tc-ibdv and bvm-ibdv) developed antibody against ibd virus (ibdv) and resisted challenge with virulent ibdv at 3 weeks of age or older. embryo vaccination did not adversely affect hatchability of chicks or survival of hatched chicks. chicks embryonally vaccinated with tc-ibdv had transient h ... | 1985 | 3008698 |
| chicken embryonal vaccination with avian infectious bronchitis virus. | a commercial infectious bronchitis virus (ibv) vaccine of the massachusetts 41 strain was injected in embryonating chicken eggs on embryonation day (ed) 18. the ibv vaccine was pathogenic for embryos, and it was passaged in chicken kidney tissue culture to reduce the pathogenicity. at the 40th tissue culture passage (p40-ibv), the virus became apathogenic for the embryos. maternal antibody-positive or -negative chicks hatching from eggs injected with p40-ibv developed antibody to ibv and were pr ... | 1986 | 3008613 |
| marek's disease virus. | 1985 | 3008524 | |
| marek's disease--a model for herpesvirus oncology. | the article will review the salient features of pathogenesis of marek's disease in terms of sequential events which occur from the time of virus entry to the development of frank lymphomas. a hypothesis will be presented which will offer a credible explanation for this specific sequence of changes. then, various factors which influence the incidence of neoplasms will be identified and the possible mechanisms by which they are influential will be discussed. these include the variable oncogenic pr ... | 1986 | 3007026 |
| different localization of the product of the v-rel oncogene in chicken fibroblasts and spleen cells correlates with transformation by rev-t. | reticuloendotheliosis virus strain t (rev-t) is a highly oncogenic avian retrovirus that transforms early lymphoid cells in vivo and in vitro, but rev-t does not transform chicken embryo fibroblasts (cef). using antisera to p59v-rel, the v-rel oncogene product of rev-t, we show that p59v-rel is expressed at equal levels and is a phosphoprotein in rev-t infected spleen cells and cef. biochemical fractionation and immunofluorescence of rev-t infected nontransformed cef show that p59v-rel is loosel ... | 1986 | 3004745 |
| expression of the oncogene of avian reticuloendotheliosis virus in escherichia coli and identification of the transforming protein in reticuloendotheliosis virus t-transformed cells. | the genome of reticuloendotheliosis virus t (rev-t) includes a unique oncogene v-rel, which is transcribed in low amounts into a 3.0-kilobase subgenomic mrna in rev-t-transformed lymphoid cells. to identify the v-rel protein, rev-t dna sequences were cloned into bacterial plasmid vectors designed to achieve expression of foreign dna sequences in escherichia coli. portions of the v-rel gene were joined to the 5' segment of the trpe gene. upon induction of trpe with indoleacrylic acid, large amoun ... | 1986 | 3003751 |
| synthesis, processing, and secretion of the marek's disease herpesvirus a antigen glycoprotein. | the 57,000- to 65,000-dalton (da) marek's disease herpesvirus a (mdhv-a) antigen glycoprotein (gp57-65) has a 47,000-da unglycosylated precursor polypeptide (pr47), as determined by immunological detection after cell-free translation of infected-cell mrna. cleavage of its signal peptide yielded a 44,000-da precursor polypeptide molecule (pr44), detected both in vivo after tunicamycin inhibition of glycosylation and in vitro after dog pancreas microsome processing of pr47. high-resolution pulse-c ... | 1986 | 3003379 |
| marek's disease in japanese quails (coturnix coturnix japonica): a study of natural cases. | marek's disease was observed in quails. gross lesions were confined mostly to the spleen and liver. microscopic lesions were commonly seen in spleen, proventriculus, liver, and duodenum. skin, peripheral nerves, and other visceral organs were also involved. of 123 quails examined, 39 had serum antibodies against marek's disease. these antibodies were detected from 11 to 17 weeks of age; the highest incidence was recorded at 15 weeks. feather follicular antigen detected in 30 of the 95 quails was ... | 1985 | 3000332 |
| [effect of the virus content per vaccination dose on the effectiveness of the immune prevention of marek's disease in general practice]. | 1985 | 3000310 | |
| production of herpesvirus of turkeys in microcarrier culturing system--a new method for production of vaccine against marek's disease. | a microcarrier (mc) culturing system for production of a vaccine against marek's disease virus is presented. cytodex-3 beads (pharmacia) and de-53 microgranular (whatman) are the recommended microcarriers. primary cells from chick embryo as well as cells from a quail cell line (qt-6, established from a japanese quail) were found to be good hosts for growth of herpesvirus of turkeys (hvt). hvt, the vaccine strain against marek's disease, was successfully grown and harvested on the above mentioned ... | 1985 | 2995176 |
| on the specificity of the immunoglobulin g produced by chickens experimentally infected with marek's disease virus. | in chickens experimentally infected with marek's disease virus (mdv) an increased amount of immunoglobulin g is produced. using a technique of quantitative crossed immunoelectrophoresis it has been shown, that 70% of this immunoglobulin g is non-specific. only 18% could be absorbed with mdv strain cprl vii-infected chicken kidney cells, and only 5% with mdv-induced lymphoblastoid cells of the mdcc-msb1 cell line. it is hypothesized that the production the unspecific immunoglobulin g is caused by ... | 1985 | 2993996 |
| genomic expansion of marek's disease virus dna is associated with serial in vitro passage. | an ecori restriction endonuclease pattern of md11 virus dna, a very virulent strain of marek's disease virus (mdv), was obtained by using total cellular dna from infected cells. with the ecori restriction endonuclease pattern and a published bamhi map of mdv (fukuchi et al., j. virol. 51:102-109), we constructed a partial ecori map of a series of mdv clones (gift from h. j. kung). the clones were used to identify a region of the md11 genome which is altered as the oncogenic virus is passaged in ... | 1985 | 2987523 |
| differences in the processing of secreted glycoprotein a induced by marek's disease virus and herpesvirus of turkeys. | the steps from the precursor to the processed forms of a secreted glycoprotein (ga) of marek's disease virus (mdv) and of herpesvirus of turkeys (hvt) were examined by two-dimensional gel electrophoresis of immunoprecipitates from the cell lysate and medium of infected cultures with monoclonal antibodies. differences between mdv- and hvt-ga were particularly observed in isoelectric points of the glycosylated or unglycosylated precursor forms. | 1985 | 2987399 |
| gel electrophoretic analysis of polypeptides from nucleocapsids of marek's disease virus strains and herpesvirus of turkey. | the polypeptides of nucleocapsids of marek's disease virus (mdv) strains with different biological properties and of antigenically related herpesvirus of turkey (hvt) strains were analysed by one- and two-dimensional (1d and 2d, respectively) gel electrophoresis. based on small differences in migration behaviour (size and charge) of a number of corresponding nucleocapsid polypeptides, the virus strains could be differentiated into three groups. the polypeptide pattern of group i, comprising the ... | 1985 | 2986577 |
| effects of chemically or virus-induced immunodepression on response of chickens to avian leukosis virus. | the effects of chemically or virus-induced immunodepression on the infection profile (development of viremia and antibody) and shedding of avian leukosis virus (alv) were studied in progeny chickens of experimental or commercial breeder flocks. chickens were infected with alv subgroup a by contact at hatching and by oral inoculation at 4-5 weeks of age. in the first experiment, chickens were inoculated with a virulent strain of infectious bursal disease virus (ibdv) at 1 day or 6 weeks of age. i ... | 1985 | 2985037 |
| the structure of marek disease virus dna: the presence of unique expansion in nonpathogenic viral dna. | dna of marek disease virus (mdv) consists of two unique regions ul and us flanked by long inverted repeat regions trl and irl, and short inverted repeat regions trs and irs, respectively, similar to herpes simplex virus dna. comparison of restriction patterns between pathogenic and nonpathogenic mdv dna was made to identify a region of viral dna different between these two types of mdv, as it may be responsible for the tumorigenicity of mdv in chickens. the results indicated that bamhi-d and -h, ... | 1985 | 2983314 |
| map location of homologous regions between marek's disease virus and herpesvirus of turkey and the absence of detectable homology in the putative tumor-inducing gene. | the dna region having homology between marek's disease virus and herpesvirus of turkey was assigned to the restriction map of marek's disease virus by southern blot hybridization. under moderate conditions at the level of 15% mismatching, homology was found to be distributed throughout the marek's disease virus genome. the long inverted-repeat regions (trl and irl), which are considered to play a significant role in tumorigenicity, did not show any homology to herpesvirus of turkey dna. | 1985 | 2983105 |
| identification of marek's disease virus-specific antigens in marek's disease lymphoblastoid cell lines using monoclonal antibody against virus-specific phosphorylated polypeptides. | for identification of the antigens specific to marek's disease virus (mdv) in virus-non-producer lymphoblastoid cell lines established from a tumor of marek's disease (md), hybridomas producing monoclonal antibodies (mabs) against the antigens were isolated. immunogens for preparation of the hybridomas were purified from the lysate of an md-lymphoblastoid cell line, msb1, by affinity chromatography coupled with chicken anti-mdv serum immunoglobulin g. three of the mabs obtained, mb1, mb2 and mb3 ... | 1985 | 2982746 |
| virus-induced atherosclerosis. herpesvirus infection alters aortic cholesterol metabolism and accumulation. | infection of normocholesterolemic, specific-pathogen-free chickens with marek's disease herpesvirus (mdv) has been shown histologically to lead to chronic atherosclerosis like that in humans. the development of herpesvirus-induced atherosclerosis in vivo and the presence of specific marek's antigen within aortic cells suggested that mdv infection may modify lipid metabolism and lead to significant lipid accumulation. experiments reported herein were designed to determine the types and quantity o ... | 1986 | 2934987 |
| marek's disease virus serotype-1 antigens a and b and their unglycosylated precursors detected by western blot analysis of infected cells. | the antigenic profile of cell cultures infected with marek's disease virus (mdv) was determined by the immunoblotting method using convalescent immune serum obtained from chickens that survived infection with mdv strain ga5. the mdv antigen profile in infected cell lysates could be accurately determined since this method has advantages over the immunoprecipitation method used in other studies. we studied six very virulent mdv isolates and the prototype of serotype 1 mdv, the ga5 strain. immunobl ... | 1988 | 2852416 |
| rna transcripts of marek's disease virus (mdv) serotype-1 in infected and transformed cells. | rna was isolated from two strains of marek's disease virus (mdv-z and mdv-b). the virus was grown in duck embryo fibroblasts (def) for 96 hr, 72 hr in the presence of phosphonoacetic acid (paa) and 24 hr in the presence of cycloheximide added at the time of infection. with the use of dna probes representing about 80% of the mdv genome, an extensive northern blot analysis of the rna was carried out. a similar analysis was done with rna extracted from the mdv-transformed cell line msb-1. this stud ... | 1988 | 2852415 |
| [isolation of the pathogenic virus of marek's disease from a flock of chickens vaccinated with the turkey herpes vaccine]. | 1988 | 2851887 | |
| some aspects of lymphomagenesis of marek's disease of chickens. | 1988 | 2848997 |