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prevalence of sexually transmitted pathogens among women attending a methadone clinic in israel.to assess the prevalence of sexually transmitted pathogens in drug-addicted women in tel aviv, israel.19912032707
chlamydia trachomatis, mycoplasma hominis and ureaplasma urealyticum infections in women. prevalence, risks and management at a south african infertility clinic.chlamydia trachomatis, mycoplasma hominis and ureaplasma urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. routine screening tests or empirical antibiotic treatment of infertile couples may be justified by the prevalence of these organisms. we studied the wives in 40 consecutive infertile couples. monoclonal direct immunofluorescence (dif) for c trachomatis was performed on fixed smears from endocervical swabs. m homini ...19912030488
use of the mycoplasma hominis 102-116 kd proteins as antigen in an enzyme-linked immunosorbent assay.mycoplasma hominis surface structures involved in human immune response and in the pathogenesis of this bacterial infection are inadequately defined. attempts have been made to identify m. hominis surface proteins, to determine the antigenicity of these polypeptides, and to examine antigens which could lead to the development of species-specific diagnostic tests. by means of western blotting, using a pool of sera from patients with culturally proven vaginal infection, most antigens recognized we ...19912014008
antepartum cultures for ureaplasma urealyticum are not useful in predicting pregnancy outcome. the vaginal infections and prematurity study group.to test the hypothesis that genital colonization with ureaplasma urealyticum would predict adverse pregnancy outcome, 4934 women from five medical centers were evaluated for vaginal colonization with u. urealyticum between 23 and 26 weeks' gestation and followed up to delivery. u. urealyticum colonization was associated with maternal age, parity, racial-ethnic group, martial status, income, education, smoking, number of sexual partners, and colonization with trichomonas vaginalis, mycoplasma hom ...19912003532
the epidemiology of group b streptococcal colonization in pregnancy. vaginal infections and prematurity study group.risk factors for cervicovaginal group b streptococcal colonization at 23-26 weeks' gestation were studied in 7742 women participating in the vaginal infections and prematurity study. the prevalence of group b streptococcus was 18.6%, and was greatest in (predominantly caribbean) hispanics from new york city, followed by blacks, whites, and other (predominantly mexican) hispanics. group b streptococcus was more common among older women and women of lower parity, and less common among women living ...19912002986
low incidence of positive amnionic fluid cultures in preterm labor at 27-32 weeks in the absence of clinical evidence of chorioamnionitis.in order to determine the utility of amniocentesis for detecting subclinical chorioamnionitis in asymptomatic afebrile women in preterm labor with intact membranes, we enrolled 47 women between 27-32 weeks' gestation in a prospective study. after enrollment, 38 women fulfilled all clinical and laboratory criteria for the study; nine women were excluded because they had a leukocyte count exceeding 15,000/microl. none of the 38 asymptomatic afebrile women had a positive culture from the amnionic f ...19911988886
comparative in vitro activity of azithromycin, clarithromycin, erythromycin and lomefloxacin against mycoplasma pneumoniae, mycoplasma hominis and ureaplasma urealyticum.the in vitro activity of three macrolides, azithromycin, clarithromycin and erythromycin and a new fluoroquinolone, lomefloxacin, against pathogenic mycoplasma (16 to 18 strains of mycoplasma pneumoniae, 41 to 77 strains of mycoplasma hominis, 65 to 104 strains of ureaplasma urealyticum) was compared. the three macrolides were highly active against mycoplasma pneumoniae. clarithromycin was the most active macrolide against ureaplasma urealyticum whereas azithromycin was somewhat more active than ...19901964899
genital mycoplasmas in preterm infants: prevalence and clinical significance.the genital mycoplasmas: ureaplasma urealyticum and mycoplasma hominis have recently assumed an increasing importance as neonatal pathogens. the aim of the present survey was to determine the prevalence of infections with these organisms in preterm infants in two neonatal intensive care units in israel. among 99 preterm infants, 24 (24%) harboured mycoplasmas in their throats shortly after birth. u. urealyticum was the most common organism. m. hominis was isolated only from 3 infants. six out of ...19911959546
the effect of ciprofloxacin and doxycycline plus metronidazole on lower genital tract flora in patients with proven pelvic inflammatory disease.alterations in the lower genital tract flora were studied during antimicrobial treatment of 36 women with acute pelvic inflammatory disease (pid). sixteen women received ciprofloxacin, and 20 received doxycycline plus metronidazole. both regimes eradicated chlamydia trachomatis in all cases and neisseria gonorrhoeae in all except one in which combination treatment failed because of resistance of the organism to tetracycline. both regimes eradicated mycoplasma hominis in all cases, while ciproflo ...19911953057
recovery of mycoplasma hominis from blood culture media.although several workers have demonstrated that mycoplasma hominis is responsible for 2 to 20% of cases of septicaemia, reports from britain do not support this premise--possibly because many of the blood culture systems used fail to sustain growth of this organism. previous work suggests that this is probably due to the inhibitory effects of sodium polyanethol sulphonate (liquoid) on m. hominis. in an extension of an in-vitro evaluation of blood culture systems, gelatin was shown to give some p ...19911943535
susceptibilities of mycoplasma hominis and ureaplasma urealyticum to two new quinolones, sparfloxacin and win 57273.mycoplasma hominis was highly susceptible to two new quinolones, with mics for 90% of isolates tested of 0.004 micrograms/ml for win 57273 and 0.063 micrograms/ml for sparfloxacin, which were activities much greater than the 1 microgram/ml found for ofloxacin and tetracycline. although ureaplasma urealyticum was less susceptible, the mics for 90% of isolates tested of 0.25 micrograms/ml for win 57273 and 0.5 micrograms/ml for sparfloxacin were four- to eightfold greater than those found for oflo ...19911929320
in vitro susceptibilities of mycoplasma pneumoniae, mycoplasma hominis, and ureaplasma urealyticum to sparfloxacin and pd 127391.the in vitro activities of two investigational quinolones, sparfloxacin (previously designated at 4140) and pd 127391, were determined for 30 strains each of mycoplasma pneumoniae, mycoplasma hominis, and ureaplasma urealyticum and compared with those of ciprofloxacin, tetracycline, clindamycin, and erythromycin. erythromycin was the most active compound against m. pneumoniae (maximum mic, less than 0.008 microgram/ml). pd 127391 (mics, less than 0.008 to 0.031 microgram/ml), sparfloxacin (mics, ...19911929260
microbiological, epidemiological and clinical correlates of vaginal colonisation by mobiluncus species.the microbiological and epidemiological correlates of vaginal colonisation by mobiluncus species were examined among randomly selected women attending a sexually transmitted disease (std) clinic. women positive for trichomonas vaginalis were excluded. mobiluncus spp. were detected by gram stained vaginal smear in 21% of 633 std clinic patients, including 53% of those with and 4% of those without bacterial vaginosis (bv), as diagnosed by clinical criteria. gardnerella vaginalis and mycoplasma hom ...19911916772
[mycoplasmas in the vaginal flora in colpitis].the vaginal secretion of 194 women was subjected to bacteriological examination. the results of cultivation examinations were compared with mop examinations. the widest bacterial spectrum was recorded in mop ii. no differences were recorded as regards detection of lactobacilli and potentially pathogenic bacteria in mop i, ii and iii. the percentage of women with ureaplasma urealyticum was equal in case of mop i, ii and iii. mycoplasma hominis was isolated significantly more frequently in mop ii ...19911913859
gonorrhea in women prostitutes: clinical data and auxotypes, serovars, plasmid contents of ppng, and susceptibility profiles.eighty-nine women prostitutes who underwent clinical and microbiologic examination were found to have gonococcal infection. the median age was 22; 92.1% were from urban areas. nearly all the women prostitutes refrained from barrier methods (92.1%) and had contact with several partners (91.0%). the most frequent clinical findings were leukorrhea (50.6%), cervicitis (20.2%), and pelvic inflammatory disease (pid) (18.0%). eighty-one women prostitutes (93.1%) had experienced a previous std, with chl ...19911902990
[a case of postpartum fever caused by mycoplasma hominis infection].a 26-year-old housewife was admitted to our hospital with a history of high fever after previous cesarean delivery. she had premature rupture of the membrane on the 41st week of pregnancy and the amniotic fluid was found to be cloudy on the fourth day after rupture. therefore, cesarean delivery was performed. on the first day of operation, her body temperature increased up to 38 degrees c in spite of the treatment with latamoxef (lmox), 3 g/day. a sample of intrauterine material yielded m. homin ...19911880452
successive synovial mycoplasma hominis isolates exhibit apparent antigenic variation.the expression of surface proteins by 14 successive mycoplasma hominis isolates obtained from the synovial fluid of a chronically infected septic arthritis patient was examined. marked differences in the expression of surface proteins, as determined by monoclonal antibodies raised against the first isolate, were observed. however, identical restriction patterns and virtually identical hybridization patterns with probes containing the conserved genes of the mycoplasma capricolum rrna operon and t ...19911879948
perinatal mycoplasmal infections.maternal infections are known to play a major role in perinatal morbidity and mortality. ureaplasma urealyticum and mycoplasma hominis are sexually transmissible microorganisms associated with a number of pathologic conditions in the pregnant woman including chorioamnionitis and postpartum fever. they are rapidly gaining recognition as significant causes of perinatal infection, especially in infants born prematurely. this review summarizes current knowledge regarding the epidemiology, pathology, ...19911879107
sequence and expression in escherichia coli of a mycoplasma hominis gene encoding elongation factor tu.we describe the molecular cloning and the complete nucleotide (nt) sequence of a mycoplasma hominis gene common to a broad range of mycoplasma species, as defined by hybridization analysis with the cloned gene. production of m. hominis protein in escherichia coli was assayed by use of a monoclonal antibody. the nt sequence analysis revealed a 1194-bp open reading frame that could encode a 43 516-da protein. computer-aided sequence comparison indicated that the gene codes for elongation factor tu ...19911864502
newly documented antimicrobial activity of quinolones.the improved antimicrobial activity of newer fluoroquinolones and novel applications recently found for the drugs already marketed are reviewed. several new compounds are more active against gram-positive bacteria than the presently marketed fluoroquinolones. win 57273, the most potent compound in vitro on a weight basis, is 16 to 128 times more active than ciprofloxacin against various staphylococci, streptococci, enterococcus spp., corynebacterium spp., listeria monocytogenes and bacillus spp. ...19911864284
[evaluation of the mycoscreen technique for the detection and quantification of genital mycoplasmas].a total of 161 samples from genital sources were evaluated using two different methods for genital mycoplasma isolation: inoculation to urea broth, arginine broth and a7 solid media (standard method) and a new enzymatic method (mycoscreen oxoid). the isolation rates for ureaplasma urealitycum were 42% (standard method) and 49% (mycoscreen) (sensitivity: 100%; specificity: 88%). the isolation rates for mycoplasma hominis were 13% (standard method) and 14% (mycoscreen) (sensitivity: 80%; specifici ...19911863619
prostaglandin production by amnion and decidual cells in response to bacterial products.media from bacterial cultures have been tested for actions on prostaglandin biosynthesis by human amnion and decidual cells. the bacterial species, which are commonly associated with intrauterine infections, were group b streptococcus, escherichia coli, fusobacterium nucleatum, bacteroides fragilis, gardnerella vaginalis, neisseria gonorrhea, mycoplasma hominis and ureaplasma urealyticum. overall, low doses of bacterial products were stimulatory of amnion prostaglandin production, whereas high d ...19911857723
recovery of mycoplasma hominis from blood culture media. 19911811128
[genital mycoplasma colonization in the female genital tract].cervical or vaginal specimens obtained from 1,178 females at different physiologic periods of life were cultured for ureaplasma urealyticum and mycoplasma hominis. low recovery rates of u. urealyticum were obtained in the neonates (20/72, 27.8%) and puerperal (22/83, 26.5%) and postmenopausal (21/70, 30.0%) women. the highest incidence (114/157, 72.6%) was observed in pregnant women. it is suggested that the colonization of genital mycoplasma is associated with the hormonal status of the women.19911804612
[age and sex distribution of sexually transmitted diseases in valladolid. a study of 5076 cases].the age and sex distribution of 5,076 cases of s.t.d. dealt with between 1982 and 1988 at the dermatological dispensary of the territorial social welfare service in valladolid are studied. the maximum frequency of s.t.d. (24.1% of cases) corresponded to the age group between 21 and 25 years, which was also the period for maximum prevalence of neisseria gonorrhoeae (26.3%), gardnerella vaginalis (30.7%), candida albicans (27.4%) and acuminata condyloma (33.4%). syphilis (19.9%), chlamydia trachom ...19911801185
induced abortion: microbiological screening and medical complications.preoperative cervical screening of 1,193 women undergoing first-trimester induced abortions yielded chlamydia trachomatis in 11.7%, neisseria gonorrhoeae in 0.8%, mycoplasma hominis in 22.1%, ureaplasma urealyticum in 10.1%, herpes simplex virus in 0.9% and group b streptococci (gbs) in 2.9%. c. trachomatis and n. gonorrhoeae were especially frequent among teenagers. a total of 2.2% (26 women) developed postoperative pelvic inflammatory disease (pid) and 0.9% (13 women) endometritis. pid develop ...19911800368
[preliminary serological survey on mycoplasma and chlamydia infection among gonorrhoea cases].the antibodies to mycoplasma hominis (mh) and chlamydia trachomatis (ct) in 123 sera from the patients with gonorrhoea and 108 sera from the healthy persons control were detected with micro-indirect hemagglutination test. the results showed that the antibody to mh positive rate was 23.58%, gmt 1: 7.60, and to ct 72.35%, gmt 1: 8.71 in the patients. the differences of the antibody levels between the patients and the controls were significant. it was suggested that the urogenital infections caused ...19911782655
an indirect immunofluorescence method for detection of mycoplasma hominis in vaginal smears.we developed a novel method for the detection of mycoplasma hominis from vaginal swabs using an indirect immunofluorescence technique. it is a rapid and simple method that can be finished in only 5 hr and is more sensitive than the usual culture isolation method. the indirect immunofluorescence method was applied to vaginal smears from 193 healthy women and 33.7% gave a positive test. this value was much higher than that (11.4%) obtained from the same specimens by the culture method. when vagina ...19911779888
emergency cervical cerclage.to assess the efficacy of emergency cervical cerclage.19911777455
[identification of mycoplasma hominis and ureaplasma urealyticum in patients with sterility of tubal origin].the peritoneal fluid of two groups of patients was cultivated; the first one was of 67 patients with tuboperitoneal problem; the second of 14 patients was the control; we founded mollicutes in 39% of the patients of the problem group and 28% of the control group. the correlation between laparoscopic aspects with the positive mollicutes cultures suggested the infection by mollicutes in the tubal cause of sterility.19911765307
detection of mycoplasma hominis antibodies by three different techniques, application to women with pelvic inflammatory disease.mycoplasma hominis, a mycoplasma potentially isolated from the genital tract of healthy women, seems to have some role in pelvic inflammatory diseases (pid). three serologic techniques were developed to detect antibodies to m. hominis: elisa (igg, igm), microimmunofluorescence (mif) and western blot. rabbit and human control sera were used to establish the standard assay conditions. elisa proved to be more sensitive but less specific than mif. cross-reactivity with antibodies to m. pneumoniae ex ...19911759730
detection of variable dna repeats in diverse eukaryotic microorganisms by a single set of polymerase chain reaction primers.we cloned and sequenced a variable dna repeat from trichomonas vaginalis, a flagellated protozoan parasite. targeting of this repeat in the polymerase chain reaction resulted in complex and intense product patterns for a wide variety of eukaryotic microorganisms, including the pathogenic protozoan parasites t. vaginalis, giardia lamblia, leishmania donovani, three species of trypanosoma, and four species of acanthamoeba; the nonpathogenic protozoans, paramecium tetraurelia and tetrahymena thermo ...19911757544
[ureaplasma urealyticum and mycoplasma hominis in male urethritis].we conducted a multi-center clinical study to evaluate the role of ureaplasma urealyticum and mycoplasma hominis in male urethritis. the incidence of each organism in first-voided urine samples of 160 male urethritis patients, including 28 with gonococcal and 126 with non-gonococcal urethritis, was investigated. u. urealyticum and m. hominis were isolated from 13.6% and 6.5%, respectively, of the urine samples, and the concentration of each mycoplasma exceeded 10(3) ccu/ml (color changing units/ ...19911755423
mycoplasma hominis infection of a breast prosthesis. 19911753125
[value and limits of research on mycoplasma hominis and ureaplasma urealyticum in the gastric fluid of newborn infants].mycoplasma hominis and ureaplasma urealyticum were cultured and counted in the gastric fluid of 153 neonates divided into three groups: 28 preterm neonates managed in an intensive care unit (group i); 83 full term neonates with suspected infection (group ii); and 42 full term neonates with no evidence of infection (group iii). the colonization rate (17.85%) in the intensive care unit group was not significantly different from the rates seen in the two other groups. these results do not militate ...19911750746
the relationship of hydrogen peroxide-producing lactobacilli to bacterial vaginosis and genital microflora in pregnant women.lactobacilli provide an important microbial defense against genital colonization by pathogens. the role of hydrogen peroxide (h2o2) in the control of genital microflora was explored in a cross-sectional study of 275 women in the second trimester of pregnancy. vaginal cultures were obtained for detection of h2o2-positive and h2o2-negative lactobacilli and other members of the genital microflora. compared with women with h2o2-negative lactobacilli, women colonized by h2o2-positive lactobacilli wer ...19921738516
the prevalence of potential pathogenic micro-organisms in the endocervix of pregnant women at tygerberg hospital.the prevalence of potential pathogenic organisms cultured from the endocervix in 206 pregnant patients was: ureaplasma urealyticum 63%, mycoplasma hominis 39%, candida albicans 20%, chlamydia trachomatis 11%, neisseria gonorrhoeae 7%, group b beta-haemolytic streptococcus 6% and listeria monocytogenes 1%. the most common complications of pregnancy found were: preterm labour 14%, urinary tract infection 8% and premature rupture of membranes 4%. significantly more patients in whom c. trachomatis o ...19921734556
mycoplasma hominis infection of perihepatic hematomas in a liver transplant recipient.we report the case of a recipient of liver transplantation in whom postoperative perihepatic hematomas were infected by mycoplasma hominis. etiologic diagnosis was delayed because this organism is a rare cause of postoperative infection and usually does not grow on standard bacteriologic media. the role of m. hominis in postoperative infections and the diagnostic problems of this organism are discussed.19921728082
monoclonal antibodies to surface antigens of a pathogenic mycoplasma hominis strain.three monoclonal antibodies (mabs) were prepared against an arthritogenic strain of mycoplasma hominis isolated from the joint aspirates of a patient with chronic septic arthritis. immunoblots of polyacrylamide gel-electrophoresed proteins before and after surface proteolysis showed that the predominant antigenic determinants were on surface-exposed polypeptides. these polypeptides have extensive hydrophobic characteristics, as demonstrated by triton x-114 phase partitioning. the electrophoresed ...19911708355
a high-affinity human monoclonal igm antibody reacting with multiple strains of mycoplasma hominis.human monoclonal antibodies were produced against mycoplasma hominis by in vitro immunization of peripheral blood lymphocytes from a healthy seropositive donor using low amounts of antigen (5 ng/ml). the immune b lymphocytes were subsequently immortalized by epstein-barr virus transformation followed by somatic cell fusion. using this procedure, several specific anti-m. hominis monoclonal antibodies of mu isotype were obtained exhibiting apparent affinity constants as high as 3 x 10(9)m-1. the p ...19901700092
fast and simple procedure for the detection of cell culture mycoplasmas using a single monoclonal antibody.the detection of mycoplasmas in cell cultures is still a problem, especially in those laboratories in which the detection and identification of microorganisms is not established as a routine procedure. in our laboratory, monoclonal antibodies (mabs) have been prepared to acholeplasma laidlawii, mycoplasma hyorhinis, mycoplasma orale, mycoplasma arginini and mycoplasma salivarium. 30 mabs were obtained and one of these, designated ccm-2, was shown to bind to all five mycoplasma species. it also b ...19901697315
in vitro activity of temafloxacin against pathogens causing sexually transmitted diseases.the fluoroquinolones represent a new therapeutic alternative for the treatment of many sexually transmitted diseases, especially those caused by neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, and urea-plasma urealyticum. in vitro studies have demonstrated the 90% minimal inhibitory concentrations (mic90s) of temafloxacin for these pathogens to be less than 0.03 micrograms/ml for n. gonorrhoeae; 0.25 micrograms/ml for c. trachomatis; 0.125-2 micrograms/ml for m. hominis; and 4 ...19911662891
temafloxacin: an overview.temafloxacin (6-fluoro-7-piperazino-4-quinolone) is a new fluoroquinolone with a 7-8 hour half-life and rapid gastrointestinal absorption. these characteristics make it an ideal antimicrobial for once- or twice-daily oral dosing. with the exception of the central nervous system (cns), temafloxacin has excellent tissue and body fluid penetration and concentration. temafloxacin has broad antimicrobial activity against gram-positive and gram-negative bacteria, including improved in vitro activity a ...19911662889
fluoroquinolones for the treatment of nongonococcal urethritis/cervicitis.approximately 50% of cases of nongonococcal urethritis reported among men are caused by chlamydia trachomatis. other pathogens implicated in the bacterial etiology of nongonococcal urethritis that occur independently or concurrently with gonorrhea include ureaplasma urealyticum and species of mycoplasma, including mycoplasma hominis. the etiology of up to half of the cases, however, remains uncertain. historically, c. trachomatis genital infections have been difficult to diagnose because of the ...19911662887
incidence of nonbacterial intraamniotic infections in abnormal pregnancies.amniotic fluids from 41 abnormal and 47 control pregnancies were examined for viruses, chlamydia trachomatis, ureaplasma urealyticum and mycoplasma hominis. abnormal pregnancies included those with fetal abnormalities, maternal complications, suspected chorioamnionitis and premature labor. two percent of cultures were positive, including cytomegalovirus from one case of fetal growth retardation and u urealyticum from two patients with chorioamnionitis and from two controls. the abnormal pregnanc ...19911662719
the in vitro activity of some 14-, 15- and 16- membered macrolides against staphylococcus spp., legionella spp., mycoplasma spp. and ureaplasma urealyticum.erythromycin is a macrolide antimicrobial chemically comprised of a 14-membered lactone ring substituted with a neutral (cladinose) and an amino (desosamine) sugar. recently, a number of new macrolide molecules have been identified containing either 14-, 15- or 16-membered substituted lactone rings. in this study the authors have determined the in vitro activity of roxithromycin and clarithromycin (both 14-membered macrolides), azithromycin (a 15-membered macrolide or azalide) and midecamycin ac ...19911650694
detection of mycoplasma contamination through modulation (stimulation or inhibition) of thymidine incorporation by unstimulated mouse spleen cells.in the present report we describe a rapid and sensitive assay for mycoplasma detection in cell cultures. the assay is based on the ability of contaminated culture supernatants to modulate [3h]tdr incorporation by unstimulated mouse splenocytes. several mycoplasma species (mycoplasma orale, culturable and non-culturable strains of mycoplasma hyorhinis) inhibited [3h]tdr incorporation and permitted the detection of some contaminated cell cultures that would otherwise have escaped detection in assa ...19921640109
[mycoplasmas and pregnancy. preliminary study].the prevalence of genital mycoplasmas was studied among 191 pregnant women followed up at diaconesses hospital, paris. ureaplasma urealyticum was recovered from 65% of patients, alone (54%) or in association with mycoplasma hominis (11%). the relationship to pregnancy outcome, the effect of erythromycin treatment, the interest of biological markers of infection (mycoplasmal quantitation and serologic tests, c-reactive protein) and the role of other known genital pathogens were investigated. vagi ...19921624724
microbial causative agents of male urethritis.the incidence of chlamydia trachomatis, ureaplasma urealyticum, mycoplasma hominis, neisseria gonorrhoeae and trichomonas vaginalis was studied in men with urethritis. out of 150 examined men 48.7% had the positive isolation of u. urealyticum, 26.0% c. trachomatis, 22.7% n. gonorrhoeae, 18.7% m. hominis and in one (0.7%) patient t. vaginalis was found. none of the above mentioned microorganisms was detected in 24.7% of examined men. in 43.3% cases only one agent was isolated. in 23.3% of the men ...19921619287
a randomized, comparative study of the efficacy and tolerance of roxithromycin and doxycycline in the treatment of women with positive endocervical cultures for chlamydia trachomatis and mycoplasma spp. in an in vitro fertilization program.a total of 45 patients wishing to enter an in vitro fertilization program had positive endocervical cultures for chlamydia trachomatis, 86 had positive cultures for ureaplasma urealyticum, and 11 had positive cultures for mycoplasma hominis. these patients and their consorts were randomly allocated to treatment with either roxithromycin or doxycycline. both antibiotics were completely effective against c. trachomatis. in patients with u. urealyticum infection, roxithromycin was successful in 73% ...19921617924
comparison of ofloxacin and metronidazole for the treatment of bacterial vaginosis.the efficacy and safety of ofloxacin, 200 mg twice daily for 7 days, was compared with metronidazole, 400 mg twice daily for 7 days, for the treatment of bacterial vaginosis (bv). diagnosis of bv was confirmed by at least 3 of the following 4 criteria: the presence of an abnormal vaginal discharge on examination, clue cells on microscopy of vaginal specimens, vaginal ph greater than 5.0 and a positive amine test. vaginal specimens were examined for mobiluncus spp, analysed for the succinate/lact ...19921616967
[ureaplasma urealyticum and mycoplasma hominis: incidence and clinical significance of their isolation in the perinatal period].in order to known the clinical significance of the colonization of the maternal genitalia and infant with genital mycoplasmas, we studied prospectively 219 pairs of mothers and newborns admitted for delivery to a general public hospital. u. urealyticum and m. hominis were isolated from 72 (32.9%) and 10 (4.6%) of the mothers, respectively. during the first 4 days of life. u. urealyticum and m. hominis were isolated from 23 (10.5%) and 1 (0.5%), of the newborn infants respectively. maternal colon ...19921605412
ovarian actinomycosis presenting as acute peritonitis.a patient with acute peritonitis due to ovarian actinomycosis and the association of this disease with the intrauterine contraceptive device is discussed. because adequate treatment requires prolonged antibiotics the diagnosis should be sure. unfortunately if antibiotics are given pre-operatively culture is usually unsuccessful and thus careful examination of all removed material is essential. as many of these women are of child bearing age surgical intervention should aim to preserve fertility ...19921586308
comparison of commercially available media for detection and isolation of ureaplasma urealyticum and mycoplasma hominis.the mycotrim triphasic flask system (irvine scientific, irvine, calif.) was compared with a system composed of mycotrim gu broth (irvine scientific) and a7 or a8 agar (remel, lenexa, kans.) for the ability to detect ureaplasma urealyticum and mycoplasma hominis from 129 genital specimens. of the 64 specimens positive for u. urealyticum, 25, 98, and 100% were detected on mycotrim triphasic agar and a7 and a8 agars, respectively. all 18 specimens that grew m. hominis were detected by a7 and a8 aga ...19921583143
[role of mycoplasmas in the last month of pregnancy and postpartum pathology. prospective study of 577 pregnancies].the authors report the findings of a prospective study of 577 pregnancies. the purpose of this study was to determine the influence of mycoplasma on peri-post-natal illness arising during the last month of gestation and during the immediate post-partum period. the incidence of contamination was 29% of ureaplasma urealyticum and 2.3% for mycoplasma hominis. this showed little change post partum. the rate of transmission to the fetus or placenta is low. no difference in end-of-term fetal weight wa ...19921579798
ureaplasma urealyticum and mycoplasma hominis in women with systemic lupus erythematosus.to determine the prevalence of genitourinary mycoplasma infection in women with systemic lupus erythematosus (sle).19921567492
adverse outcome in pregnancy following amniotic fluid isolation of ureaplasma urealyticum.infections in pregnancy with ureaplasma urealyticum have been associated with a wide range of adverse outcomes, such as early abortion, stillbirth, prematurity, and neonatal morbidity and mortality. causality has been difficult to demonstrate secondary to the high prevalence of asymptomatic lower genital tract (lgt) colonization and culture data from inaccessible or potentially contaminated sites. between 1985 and 1989, 2461 second-trimester genetic amniocenteses were evaluated at the cytogeneti ...19921553356
physical and genetic mapping of the genomes of five mycoplasma hominis strains by pulsed-field gel electrophoresis.we present the complete maps of five mycoplasma hominis genomes, including a detailed restriction map and the locations of a number of genetic loci. the restriction fragments were resolved by field inversion gel electrophoresis or by the contour-clamped homogeneous-electric-field system of pulsed-field gel electrophoresis. all the apai, smai, bamhi, xhoi, and sali restriction sites (total of 21 to 33 sites in each strain) were placed on the physical map, yielding an average resolution of 26 kb. ...19921551841
the prevalence and clinical significance of intraamniotic infection with candida species in women with preterm labor.intraamniotic infection is considered a major etiologic factor of preterm birth. positive amniotic fluid cultures are rarely contaminated with candida species. the presence of this microorganism is associated with a poor pregnancy outcome. out of 773 transabdominal amniocenteses performed in women presenting with preterm labor and intact membranes, 77 patients (9.9%) had positive amniotic fluid cultures and in 5 women (6.5%) candida species were identified. on the other hand, 625 amniocenteses w ...19921550392
lumbar pain caused by mycoplasma infection.a 45-year-old woman was admitted to hospital following acute onset of lower back pain. clinical and laboratory investigations established a lumbar paraspinal soft tissue infection with mycoplasma hominis associated with severe spondylarthrosis at l5/s1. a relationship to a recently performed hysterectomy must be considered.19921533852
rapid detection of tetm in mycoplasma hominis and ureaplasma urealyticum by pcr: tetm confers resistance to tetracycline but not necessarily to doxycycline.tetracycline resistance in mycoplasma hominis and ureaplasma urealyticum has been associated with the tetm determinant and has recently been increasing in incidence. we report here a rapid method for detection of the tetm determinant based on the use of the polymerase chain reaction (pcr) to amplify a 397-bp dna fragment from the tetm gene and verification of specificity using the restriction enzyme taqi. analysis of 42 u. urealyticum and 49 m. hominis isolates indicates that the pcr method may ...19921526460
nucleotide sequence of the arginine deiminase gene of mycoplasma hominis.the arginine deiminase gene of mycoplasma hominis was amplified by the polymerase chain reaction, and its entire nucleotide sequence was determined. this gene consists of 1227 base pairs encoding 409 amino acids, and has 35.2% guanine plus cytosine content. nucleotide sequence homologies of the arginine deiminase gene between m. hominis and m. arginini, and between m. hominis and m. orale were 82.1 and 80.8%, respectively, suggesting that this gene is highly conserved among arginine-utilizing my ...19921522817
prerequisite work-up of the couple before in-vitro fertilization.prerequisite examinations, including immune status for rubella and hepatitis b antigens, cultures for chlamydia trachomatis and mycoplasmas, pap smear and hystersalpingogram were performed in 227 couples before their enrollment into an in-vitro fertilization (ivf) programme. the examinations were completed in 187 couples. immune status for rubella had already been documented in 45% of the women: of the remainder, 11 patients were not immune (6%). a screening test for hepatitis b antigens had alr ...19921522189
septic arthritis and bacteremia due to mycoplasma resistant to antimicrobial therapy in a patient with systemic lupus erythematosus.we report a case of septic arthritis caused by mycoplasma hominis in a patient with systemic lupus erythematosus. the infection started as monarthritis but spread to at least four joints despite apparently suitable therapy with various antimicrobial agents, including doxycycline, clindamycin, and ciprofloxacin; subsequent bacteremia was documented. control was ultimately achieved with use of the experimental fluoroquinolone temafloxacin in combination with doxycycline administration, arthroscopi ...19921520784
mycoplasma hominis in women with bacterial vaginosis.an association between m. hominis and bacterial vaginosis (bv) was looked for in total 205 women characterized as having bv. vaginal specimens from these patients were studied for the presence of amines, polymorphonuclear leukocytes, clue cells, and lactobacilli, and were also cultured to isolate gardnerella vaginalis, m. hominis, trichomonas vaginalis, candida albicans and anaerobes. g. vaginalis was found to be the most common isolate from 132 cases (64.3%). m. hominis was isolated from 90 cas ...19921506065
gardnerella vaginalis: characteristics, clinical considerations, and controversies.the clinical significance, gram stain reaction, and genus affiliation of gardnerella vaginalis have been controversial since gardner and dukes described the organism as the cause of "nonspecific vaginitis," a common disease of women which is now called bacterial vaginosis. the organism was named g. vaginalis when taxonomic studies showed that it was unrelated to bacteria in various genera including haemophilus and corynebacterium. electron microscopy and chemical analyses have elucidated the org ...19921498765
a longitudinal study of pelvic inflammatory disease.to study the microbiology and long term prognosis of pelvic inflammatory disease (pid).19921477024
[immunoreactivity of mycoplasma hominis pg21 antigens and human sperm antigens].the authors selected from the who bank for reproductive immunology ten sera containing concurrently antispermatozoal antibodies and antibodies against mycoplasma hominis pg21. by absorption examination of selective sera with a different concentration (10(9)/ml, 3.10(8)/ml, 10(8)/ml) of human spermatozoa and a rinsed sonicate of mycoplasma hominis pg21 in dilutions of 1:20, 1:80, 1:320 the authors did not find a crossed immunoreactivity between sperm antigens and mycoplasmic antigens.19921473166
in vitro activity of several antimicrobial agents against genital mycoplasmas.the increasing resistance of genital mycoplasmas to tetracyclines is a serious problem, since this group of antibiotics is one of the few that is effective against virtually all species of mycoplasmas. tetracyclines are also used to treat many sexually transmitted diseases. in this study, we assessed the susceptibility of mycoplasma hominis and ureaplasma urealyticum to macrolides, tetracyclines, spectinomycin, and trospectomycin by the agar dilution method. for m hominis, trospectomycin was the ...19921468088
quinolones as an alternative treatment of chlamydial, mycoplasma and gonococcal urogenital infections.we report the results of several trials aimed at evaluating the quinolones in urogenital infections. in chlamydia trachomatis infections, ofloxacin (200 mg b.i.d. for 10 days) gave a cure rate of 98% (n = 66), and fleroxacin (400 mg s.i.d. for 7 days) provided a cure rate of 89% (n = 19). a double-blind study comparing fleroxacin (600 mg s.i.d.) to doxycycline (100 mg b.i.d.) for 7 days showed similar high cure rates for both regimens (100%; n = 23). in mycoplasma hominis infections, ofloxacin ( ...19921421625
fatal intrauterine infection associated with mycoplasma hominis. 19921420708
[significant bacteriospermia. value and limits of sperm count in andrology].the data concerning bacteriospermia--are very different in the literature. asymptomatic andrologic patients, and as control group--patients of the outpatient department for male adnexitis--were studied. cultivation of aerobic bacteria and mycoplasmas has been recently accomplished by quantitative bacteriological analysis and chlamydia diagnostic. the microbiological screening of 210 andrologic patients ejaculate has given the following results: 19.6% were sterile, 21.0% were colonised by apathog ...19921408103
[epidemic investigation of genital mycoplasma hominis infection in women].the genital mycoplasma hominis infection in women was investigated at xiaguan district, nanjin in june, 1990. leucorrheas from 722 women were tested with elisa for the antigen of m. hominis. the prevalences of genital m. hominis were not statistically different among age and occupation groups. women having pregnancy more than three times had a significantly higher infection rate, it is suggested that induced abortion might provide chances of the infection. the prevalence was significantly differ ...19921394388
[epidemiological factors and vaginal flora changes in vaginal bacteriosis (bacterial vaginosis)].the aim of this study was to know the modifications of the vaginal bacterial flora that occurs in bacterial vaginosis and to know the involvement of these microorganisms and the influence of several epidemiologic factors in the etiology of this disease.19921391016
decreased metabolism and viability of mycoplasma hominis induced by monoclonal antibody-mediated agglutination.monoclonal antibodies (mabs) were generated against lysates of clinical mycoplasma hominis isolates. three of these, designated bg2, ba10, and fe6, recognized an integral membrane protein of m. hominis with an apparent molecular weight of 50,000 (p50). electron microscopy studies demonstrated that this protein is distributed evenly over the cell surface. these anti-p50 mabs were species specific for m. hominis; they reacted with 42% of 126 tested clinical m. hominis isolates and showed no reacti ...19921370272
lack of activity of zidovudine against ureaplasma urealyticum and mycoplasma hominis. 19921354810
vaginal microbial flora as a cofactor in the pathogenesis of uterine cervical intraepithelial neoplasia.the vaginal microbial flora of 106 women with histopathologically confirmed cervical intraepithelial neoplasia and 79 women without disease, was evaluated for gardnerella vaginalis, trichomonas vaginalis, candida albicans and other yeasts. flora morphology was assessed by gram staining of secretions. cervical cultures were examined for herpes simplex virus, cytomegalovirus and neisseria gonorrhoeae. chlamydia trachomatis antigens in cervical secretions were detected by enzyme immunoassay. human ...19921351005
in vitro activity of sparfloxacin and other antimicrobial agents against genital pathogens.the in vitro activity of sparfloxacin was determined for 60 strains of neisseria gonorrhoeae, 15 strains of chlamydia trachomatis and 40 strains each of gardnerella vaginalis, mycoplasma hominis, and ureaplasma urealyticum and compared with those of ampicillin, azithromycin, clarithromycin, erythromycin, ofloxacin, temafloxacin and tetracycline. sparfloxacin was active against all the strains studied and appeared to be the most potent quinolone tested. sparfloxacin had the lowest mics against n. ...19921337506
high incidence of asymptomatic urogenital infection in patients with uveitis anterior.acute anterior uveitis (aau) may be associated with systemic infectious or inflammatory disease. we examined 92 patients with the first attack of acute anterior uveitis; all patients were free of any extraocular symptoms. a thorough clinical examination did not reveal any systemic underlying disease. in the course of microbiological examination, however, a high incidence of asymptomatic infection of the urethra and/or cervix with ureaplasma urealyticum, chlamydia trachomatis and mycoplasma homin ...19921303857
[epidemiological study on mycoplasmas colonization and infection in the female genital tract].1309 cervical or vaginal specimens from females in various physiological periods and women with multiple sexual partners were examined for ureaplasma urealyticum and mycoplasma hominis from feb. to may, 1990. significantly, low carriage rates of u.urealyticum were found in the neonatal (28.57%), puerperium (26.51%) and postmenopause (30.00%) groups, whereas, pregnant women (more than 36 weeks) showed the highest incidence (73.20%). the recovery rate of u. urealyticum in girls with no history of ...19921303321
[study on the infections of u. urealyticum, m. hominis, and c. trachomatis in patients with venereal diseases and healthy controls in three areas of china].two hundred and thiry-nine patients with venereal diseases and 264 healthy controls, from yichang, liuzhou, and beijing, were detected for the antibodies to ureaplasma urealyticum (uu). mycoplasma hominis (mh), and chlamydia trachomatis (ct) by indirect hemagglutination (iha) in 1990. the results showed that: 1) in patients with venereal diseases, the positive rates was the highest in ct (27.62%), higher in uu (17.57%), and lower in mh (4.18%); 2) the levels of antibody to ct and uu were signifi ...19921301266
[uses of vaginal ph measurement for prevention of premature labor. results of a prospective study].the influence of the microflora of the vagina and cervix has been undervalued for the induction of premature birth. examination of the microbiological problem by determination of the vaginal ph in combination with the microflora (chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum). report of the outcome of 161 prospective, ad the beginning normal pregnancies in comparison of 200 primary hospitalized pregnancies with symptoms of imminent premature birth. conclusion: the vaginal ph ...19921290278
[sensitivity of mycoplasma hominis cultures isolated from clinical material to certain antibiotics].sensitivity to ii antibiotics of 80 strains of m. hominis isolated from patients with various inflammatory processes of the urogenital tract was studied by the method of suppressing the metabolic activity. inhibition of the arginine metabolism of mycoplasma was used as a test for determination of the growth suppression. all the strains tested were highly sensitive to tetracycline and lincomycin. kanamycin and neomycin were less active against m. hominis. all the strains tested were resistant to ...19761275455
development of an animal model for mycoplasma-related reproductive failure.pregnant hamsters and mice were injected ip with various doses of mycoplasma hominis, m fermentans, and m arthritidis, all of which may have a potential role in human or animal reproductive failure. inoculations were made near the completion of each trimester, and the products of conception were removed by hysterectomy 24 hr before anticipated delivery. resorptions and birth defects were tabulated. preliminary data on 1869 fetuses indicate that balb mice merit further evaluation as a possible mo ...19761271738
the role of mycoplasma in human infertility.seminal fluid from males complaining of infertility was examined for the presence of t-mycoplasmas and mycoplasma hominis. no significant difference, with regard to the presence of mycoplasmas, was found between the seminal fluid which had normal cytology and seminal fluid of which the cytology was deemed pathological. colonisation was shown to be an evanescent phenomenon, and female consorts often had different mycoplasma flora to the males. no positive role of mycoplasmas in the pathogenesis o ...19761265543
serological studies on mycoplasma hominis: serotypic distribution of recent clinical isolates.serotyping of strains of mycoplasma hominis, freshly isolated from various sources, was conducted using the complement-independent growth agglutination (ga) test, using the 7 reference antisera prepared in our laboratory. multiple isolates from a common source indicated that only a single serotype tends to be found, making it possible to serotype clinical isolates without further cloning. however, cultures collected at different times from the same individual may contain different serotypes. a t ...19761265446
t-strain mycoplasma in the chimpanzee.specimens from 4 chimpanzees were cultured for t-strain mycoplasma and mycoplasma hominis. t-strain mycoplasmas were recovered from the genital tract and throat of a male and the genital tract of his female cagemate; neither had clinical evidence of infection. two other male chimpanzees were culturally negative for t-strain mycoplasmas. m hominis was not isolated from any of the animals. the chimpanzee may serve as a suitable experimental model for studying the role of t-strain mycoplasmas in hu ...19761263431
role of arginine deiminase in growth of mycoplasma hominis.arginine has been considered as the major energy source of nonglycolytic arginine-utilizing mycoplasmata. when three strains of mycoplasma arginini, and one strain each of mycoplasma arthritidis, mycoplasma fermentans, mycoplasma gallinarum, mycoplasma gallisepticum and mycoplasma hominis were grown in the medium with high arginine concentration (34 mm) compared with low arginine (4 mm), both the protein content of the organisms and the specific activity of arginine deiminase increased. m. ferme ...19761262306
studies on ciliated epithelia of the human genital tract. i. swelling of the cilia of fallopian tube epithelium in organ cultures infected with mycoplasma hominis.organ cultures of human fallopian tubes were infected with mycoplasma hominis. scanning and transmission electron microscopy revealed swelling of the cilia of the tubal epithelial cells in infected cultures. in some, the entire cilia were swollen; in others, only the tips. uninfected cultures kept for up to 7 days showed no structural changes in the cilia or other surface structures. m. hominis multiplied in organ cultures, but not in culture medium without tissue. a practical organ culture tech ...19761260408
characterization of the mycoplasma membrane proteins. vi. composition and disposition of proteins in membranes from aging mycoplasma hominis cultures.membranes of mycoplasma hominis cells from cultures progressing from the mid to the end of the logarithmic phase of growth became richer in protein, poorer in phospholipids and cholesterol, heavier in density, and more viscous as determined by epr. the membrane-bound atpase activity declined steeply. electrophoretic analysis failed to show marked changes in membrane protein composition on aging, apart from an increase in the staining intensity of one protein band (mr approximately 130 000) conco ...19761252508
the anti-mycoplasmal activity of aminosidine.some in vivo experiments of therapeutic activity in severe experimental infection (intracranial) in the mouse due to different strains of mycoplasma proved the high efficacy of the antibiotic aminosidine when administered subcutaneously at the dose of 30 mg/kg and have also demonstrated that oral treatment is moderately effective against mycoplasma hominis 2. sensitivity test carried out in vitro on the same mycoplasma strains confirmed the bactericidal activity of aminosidine. these experiments ...19751204831
failure to show mycoplasmas and cytopathogenic virus in rheumatoid arthritis.synovial needle biopsies, joint aspirates, and joint tissue obtained at open operation from 41 cases of rheumatoid arthritis were inoculated onto pplo media, l-form medium, and cell cultures for the isolation of mycoplasmas, l-form bacteria, and viruses. medium suitable for the isolation of 't' strain mycoplasmas was not employed. no mycoplasmas, l-form bacteria, or cytopathogenic viruses were shown. similar specimens from nine patients diagnosed as having reiter's disease were examined in a lik ...19751190856
prevalence of mycoplasma hominis and ureaplasma urealyticum (t strains) in urine of adolescents.adolescent children were surveyed for colonization with mycoplasma hominis and ureaplasma urealyticum by culturing urine specimens. rates were compared between three study groups: (i) 397 children attending parochial schools, (ii) 293 children attending an adolescent clinic specializing in adjustment problems, and (iii) 86 children attending a renal clinic. the recovery rate was higher among postpubertal girls attending the renal clinic (33%) and the adolescent clinic (26%) than among students a ...19751176630
chorioamnionitis and colonization of the newborn infant with genital mycoplasmas.to study the role of mycoplasma hominis and t-mycoplasmas (ureaplasma urealyticum) in chorioamnionitis, we obtained culture from 249 puerperal women and their babies. the placentas were examined histologically. infants whose placentas showed inflammation (chorioamnionitis) had cultures positive for t-mycoplasmas more frequently (37.5 per cent) than those with normal placentas (19.0 per cent) (p = 0.021). colonization with m. hominis was found in 16.0 per cent of the babies and was not significan ...19751168854
immunological studies on the localization of phosphatidylglycerol in the membranes of mycoplasma hominis.phosphatidylglycerol is the main component (87%) of the membrane phospholipids of mycoplasma hominis. it is immunologically active. antibodies directed against phosphatidylglycerol were detected in rabbits intravenously immunised with native m. hominis or isolated m. hominis membranes. the intravenous method of immunisation was chosen in order to select for a response to surface antigenic determinants. anti-phosphatidylglycerol antibodies were induced in rabbits by intravenously injecting the fl ...19751158331
mycoplasma meningitis in infants with spina bifida.infants with myelomeningocele are liable to develop bacterial colonisation of the lesion which, in the case of gram-negative bacteria, often progresses to meningitis because of the infants' immature immune status. the time and origin of the bacterial colonisation usually are uncertain. this study reports seven cases of meningitis caused by mycoplasma hominis, an organism not previously reported to cause meningitis. the commensal status of mycoplasma hominis in the vagina of many women, and its i ...19751107109
mycoplasma hominis: growth, reproduction, and isolation of small viable cells.improved methods for studying the growth of mycoplasma hominis (atcc 14027) have been developed, involving modified growth conditions and preparation of the organisms under minimally distorting conditions. cells so prepared from batch cultures show relatively uniform exponential growth and appear to be dividing by binary fission; but pleomorphic forms appear upon further incubation. similar behavior was demonstrated by another laboratory-adapted strain and by three clinical isolates, and therefo ...19751102522
microbial flora of the lower genital tract during pregnancy: relationship to morbidity.nineteen genera and groups of micro-organisms were isolated from the lower genital tract of 280 women at their first antenatal visit. chlamydia, viruses, and t-strain mycoplasmas were not sought, and only routine methods of anaerobic culture were used. growth was recorded as scanty, moderate or heavy. the population studied was grouped according to age, parity, gestational stage at booking, presence and degree of severity of lower genital tract morbidity, past history of vulvovaginitis, and susp ...19751100681
combined type-specific antisera in the identification of mycoplasma hominis.in the usual serologic test for mycoplasma hominis, disks containing antibody to a prototype strain are applied to an agar plate that has been inoculated with the microorganism to be tested, and inhibition of growth is studied. the recent demonstration that m. hominis is not serologically homogeneous but consists of at least seven serotypes suggested the need for reexamination of the specificity of the disk test. the inhibition of growth of m. hominis on solid medium by disks containing seven an ...19751094075
[further investigations for the demonstration of a specific microorganism in sympathetik ophthalmia].19 following the inoculation of tissue culture cells with material obtained from 3 human eyes suffering from sympathetic ophthalmia (2 sympathizing = sy-l, 1 sympathized = sy-2), no growth of bacteria was observed. on the other hand, changes occurred such as are typical for a massive infection with mycoplasmas. the morphology of these microbes corresponds, down to the details, with micro-organisms which the author has frequently demonstrated and illustrated since 1948 in human, chicken and monke ...19751091180
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