Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| enantioselectivity of epoxide hydrolase catalysed oxirane ring opening: a 3d qsar study. | a 3d qsar analysis (quantitative structure activity relationships) of a set of 2,2-disubstituted epoxides, substrates for epoxide hydrolases originating from four different organisms, was conducted by comfa (comparative molecular field analysis) and comsia (comparative molecular similarity indices analysis), with respect to the enantioselective ring opening to the corresponding vicinal diol. structural variations of the substrates include alkyl chains of different lengths, unsaturated moieties ( ... | 2003 | 12926852 |
| nitrile biotransformations for the synthesis of enantiomerically enriched baylis-hillman adducts. | catalysed by the nitrile hydratase/amidase-containing rhodococcus sp. aj270 cells, a number of beta-aryl- and beta-alkyl- beta-hydroxy-alpha-methylenepropiononitriles (the baylis-hillman nitriles) 1 underwent hydrolysis under mild conditions to produce the corresponding enantiomerically enriched baylis-hillman amides 2 and acids 3. the enantioselectivity of the biotransformations was strongly determined by the steric effect of the substituents at the beta-position of the substrates. the protecti ... | 2003 | 12926256 |
| complete nucleotide sequence and genetic organization of the 210-kilobase linear plasmid of rhodococcus erythropolis bd2. | the complete nucleotide sequence of the linear plasmid pbd2 from rhodococcus erythropolis bd2 comprises 210,205 bp. sequence analyses of pbd2 revealed 212 putative open reading frames (orfs), 97 of which had an annotatable function. these orfs could be assigned to six functional groups: plasmid replication and maintenance, transport and metalloresistance, catabolism, transposition, regulation, and protein modification. many of the transposon-related sequences were found to flank the isopropylben ... | 2003 | 12923100 |
| analysis of anamnestic immune responses in adult horses and priming in neonates induced by a dna vaccine expressing the vapa gene of rhodococcus equi. | rhodococcus equi remains one of the most important pathogens of early life in horses, yet conventional vaccines to prevent rhodococcal pneumonia have not been successful. dna vaccination offers an alternative to conventional vaccines with specific advantages for immunization of neonates. we developed a dna vaccine expressing the vapa gene (pvr1055vapa) that induced an anamnestic response characterized by virulence associated protein a (vapa)-specific igg antibodies in sera and bronchoalveolar la ... | 2003 | 12922115 |
| microbiological transformation of benzene into phenol by cultured rhodococcus erythropolis 3/89 cells. | 2001 | 12918356 | |
| degradation of car engine base oil by rhodococcus sp. ndkk48 and gordonia sp. ndky76a. | two microorganisms (ndkk48 and ndky76a) that degrade long-chain cyclic alkanes (c-alkanes) were isolated from soil samples. strains ndkk48 and ndky76a were identified as rhodococcus sp. and gordonia sp., respectively. both strains used not only normal alkane (n-alkane) but also c-alkane as a sole carbon and energy source, and the strains degraded more than 27% of car engine base oil (1% addition). | 2003 | 12913308 |
| novel bacteria degrading n-acylhomoserine lactones and their use as quenchers of quorum-sensing-regulated functions of plant-pathogenic bacteria. | bacteria degrading the quorum-sensing (qs) signal molecule n-hexanoylhomoserine lactone were isolated from a tobacco rhizosphere. twenty-five isolates degrading this homoserine lactone fell into six groups according to their genomic rep-pcr and rrs pcr-rflp profiles. representative strains from each group were identified as members of the genera pseudomonas, comamonas, variovorax and rhodococcus: all these isolates degraded n-acylhomoserine lactones other than the hexanoic acid derivative, albei ... | 2003 | 12904538 |
| the thiocarbamate-inducible rhodococcus enzyme thcf as a member of the family of alpha/beta hydrolases with haloperoxidative side activity. | purified thiocarbamate-inducible thcf of rhodococcus erythropolis ni86/21, overexpressed in escherichia coli, displayed several characteristics of the hash family of enzymes that groups prokaryotic proteins of the alpha/beta hydrolase superfamily possessing serine-dependent hydrolase and/or haloperoxidase activity. kinetic analysis of bromination and ester hydrolysis revealed a low affinity of thcf for model substrates. sulfoxidation of thiocarbamates was demonstrated but probably represents a s ... | 2003 | 12892883 |
| [bacterial infections in patients infected by hiv]. | individuals infected by the human immuno deficiency are more prone to suffering certain bacterial infections in the course of their clinical evolution. the agents involved in these infections are: streptococcus pneumoniae, haemophilus influenzae, pseudomonas aeruginosa, salmonella spp and campylobacter spp, which occasion an increase in morbidity and mortality. with a lower frequency, but with an equal morbidity and mortality, infections have been found caused by rhodococcus equi, nocardia spp a ... | 1998 | 12891392 |
| cloning of a rhodococcal promoter using a transposon for dibenzothiophene biodesulfurization. | the expression of biodesulfurization genes (dsz) in rhodococcus erythropolis strain ka2-5-1 is repressed by sulfate which is the product of biodesulfurization. the application of a sulfate non-repressible promoter could be effective in enhancing biodesulfurization. a promoter-probe transposon was constructed using the promoterless, red-shifted green fluorescence protein gene (rsgfp). a 340 bp putative promoter element, designated kap1, was isolated from a strain ka2-5-1 recombinant that had show ... | 2003 | 12882585 |
| effects of culture conditions on the mycolic acid composition of isolates of rhodococcus spp. from activated sludgefoams. | the influence of two different carbon sources and three incubation temperatures on the mycolic acid compositions of three rhodococcus isolates from activated sludge was examined using selective ion monitoring (sim) gas chromatography-mass spectrometry (gc-ms). considerable qualitative and quantitative differences were detected in the mycolic acid compositions of the three very closely related isolates grown under the same conditions. culture age also affected both the chain lengths and proportio ... | 2003 | 12866841 |
| color removal ability of a streptomycin resistant decolorizing strain rhodococcus erythropolis (atcc:4277.1). | rhodococcua erythropolis (atcc 4277.1) is a streptomycin resistant mutant of atcc 4277, which can decolorize the sulfonated azo dye of orange ii and amido black. pseudomonaes luteola is a decolorizing strain, which was isolated from sludge resulting from the treatment of dyeing wastewater. this study had two purposes: 1) to determine the color removal capacity of r. erythropolis (atcc 4277.1) for red 22, v2rp and rp2b dyes; and 2) to compare the decolorization capability of r. erythropolis (atcc ... | 2003 | 12862232 |
| isolation and characterization of a transposon mutant of pseudomonas aeruginosa affecting uptake of dibenzothiophene in n-tetradecane. | isolation and characterization of a transposon mutant of pseudomonas aeruginosa affecting the uptake of dibenzothiophene (dbt) in n-tetradecane (n-td). | 2003 | 12859648 |
| stereo- and regiospecific cis,cis-muconate cycloisomerization by rhodococcus rhodochrous n75. | cis,cis-muconate cycloisomerase was purified to homogeneity from cells of rhodococcus rhodochrous n75 grown at the expense of benzoate and p-toluate as the sole sources of carbon. a single cycloisomerase was found to be induced in this organism with no isoforms being detected when r. rhodochrous n75 was grown on either benzoate or p-toluate as the sole source of carbon. the enzyme is hexameric with a single subunit mr of 40,000. cis,cis-muconate cycloisomerase from r. rhodochrous n75 displayed s ... | 2003 | 12855164 |
| [bacteraemia in patients with hiv disease: aetiological spectrum and profile of antimicrobial susceptibility]. | a retrospective survey of non-opportunistic bacterial pathogens isolated from blood cultures of patients with hiv disease has been carried out for a 6-year period, and the antibiotic susceptibility of the 748 microorganisms cultured from 682 consecutive patients, has been evaluated. gram-positive organisms significantly prevailed over gram-negative ones, with staphylococcus aureus, coagulase-negative staphylococci, enterococcus faecalis, xantomonas maltophilia, salmonella and pseudomonas sp. as ... | 1997 | 12847308 |
| conversion of aliphatic 2-acetoxynitriles by nitrile-hydrolysing bacteria. | the enzymatic hydrolysis of the nitrile group of different 2-acetoxynitriles was investigated in order to obtain catalysts that chemoselectively hydrolyse nitriles in the presence of ester groups. the biotransformation of four 2-acetoxynitriles [2-acetoxybutenenitrile (abn), 2-acetoxyheptanenitrile (ahn), 2-acetoxy-2-(2-furyl)acetonitrile (afn), and 2-acetoxy-2,3,3-trimethylbutanenitrile (atmb)] by different bacterial strains that synthesise nitrilases or nitrile hydratases was studied. abn, ahn ... | 2003 | 12845494 |
| rapid identification of rhodococcus equi by a pcr assay targeting the choe gene. | the actinomycete rhodococcus equi is an important pathogen of horses and an emerging opportunistic pathogen of humans. identification of r. equi by classical bacteriological techniques is sometimes difficult, and misclassification of an isolate is not uncommon. we report here on a specific pcr assay for the rapid and reliable identification of r. equi. it is based on the amplification of a fragment of the choe gene encoding cholesterol oxidase. the choe-based pcr was assessed by using a panel of ... | 2003 | 12843070 |
| a small cryptic plasmid from rhodococcus erythropolis: characterization and utility for gene expression. | exploration of metabolically diverse rhodococci is generally hampered by the lack of genetic tools. a small cryptic plasmid (pan12) isolated from rhodococcus erythropolis strain an12 was sequenced. plasmid pan12 encodes proteins that share homology to replication proteins and putative cell division proteins. based on in vitro transposon mutagenesis, we determined that the rep protein of pan12 is essential for plasmid replication in rhodococcus spp., and the putative cell division protein div is ... | 2003 | 12835922 |
| steady-state and pre-steady-state kinetic analysis of halopropane conversion by a rhodococcus haloalkane dehalogenase. | haloalkane dehalogenase from rhodococcus rhodochrous ncimb 13064 (dhaa) catalyzes the hydrolysis of carbon-halogen bonds in a wide range of haloalkanes. we examined the steady-state and pre-steady-state kinetics of halopropane conversion by dhaa to illuminate mechanistic details of the dehalogenation pathway. steady-state kinetic analysis of dhaa with a range of halopropanes showed that bromopropanes had higher k(cat) and lower k(m) values than the chlorinated analogues. the kinetic mechanism of ... | 2003 | 12834356 |
| regioselective oxidation of xylene isomers by rhodococcus sp. strain dk17. | rhodococcus sp. strain dk17 is able to utilize a variety of monocyclic aromatic hydrocarbons, including benzene, phenol, toluene, and o-xylene, as growth substrates. although dk17 is unable to grow on m- and p-xylene, this strain could transform these two xylene isomers to some extent after induction by o-xylene. the major accumulating compounds formed during the degradation of m- and p-xylene by dk17 were isolated by high-pressure liquid chromatography and identified by gas chromatography-mass ... | 2003 | 12829288 |
| molecular characterization of dioxygenases from polycyclic aromatic hydrocarbon-degrading mycobacterium spp. | polycyclic aromatic hydrocarbon (pah)-degrading genes nida and nidb that encode the alpha and beta subunits of the aromatic ring-hydroxylating dioxygenase have been cloned and sequenced from mycobacterium vanbaalenii pyr-1 [khan et al., appl. environ microbiol. 67 (2001) 3577-3585]. in this study, the presence of nida and nidb in 12 other mycobacterium or rhodococcus strains was investigated. initially, all strains were screened for their ability to degrade pahs by a spray plate method, and for ... | 2003 | 12829283 |
| a ph-indicator-based screen for hydrolytic haloalkane dehalogenase. | 2003 | 12824585 | |
| pathologic quiz case: a 15-year-old adolescent girl with a cavitary lesion in the lung. | 2003 | 12823069 | |
| transformation of rhodococcus rhodnii, a symbiont of the chagas disease vector rhodnius prolixus, with integrative elements of the l1 mycobacteriophage. | elimination of vector populations through the use of insecticides is the principal means of controlling chagas disease. because of the limitations of insecticide use, we have been developing a new potential method of control, to be used in conjunction with insecticide programs, a method which utilizes genetically modified symbiotic bacteria. these transformed bacteria can express anti-parasitic agents in the gut of the bug where the trypanosomes also are found. previous studies have shown that i ... | 2003 | 12809804 |
| purification and characterization of the aromatic desulfinase, 2-(2'-hydroxyphenyl)benzenesulfinate desulfinase. | 2-(2(')-hydroxyphenyl)benzenesulfinate desulfinase (hpbs desulfinase) catalyzes the cleavage of the carbon-sulfur bond of 2-(2(')-hydroxyphenyl)benzenesulfinate (hpbs) to form hydroxybiphenyl and sulfite. this is the final step in the desulfurization of dibenzothiophene, the organosulfur compound used to study biodesulfurization of petroleum middle distillate. hpbs desulfinase was purified 1600-fold from rhodococcus igts8. the purification was monitored using a spectrofluorimetric assay and sds- ... | 2003 | 12801508 |
| characterization of the iron-regulated desa promoter of streptomyces pilosus as a system for controlled gene expression in actinomycetes. | background: the bioavailability of iron is quite low since it is usually present as insoluble complexes. to solve the bioavailability problem microorganisms have developed highly efficient iron-scavenging systems based on the synthesis of siderophores that have high iron affinity. the systems of iron assimilation in microorganisms are strictly regulated to control the intracellular iron levels since at high concentrations iron is toxic for cells. streptomyces pilosus synthesizes the siderofore d ... | 2003 | 12801423 |
| evidence of cytochrome p450-catalyzed cleavage of the ether bond of phenoxybutyrate herbicides in rhodococcus erythropolis k2-3. | bacterial strain rhodococcus erythropolis k2-3 can cleave the ether bond of the phenoxybutyrate herbicides, i.e., 4-(2,4-dichlorophenoxy)butyrate (2,4-db) and 4-(4-chloro-2-methylphenoxy)butyrate (mcpb), by an enzyme system that is constitutively expressed. the enzyme(s) involved were investigated in this study. the rate of disappearance of 2,4-db determined in a whole cell assay amounted to 0.6 mmol/h x g(dry mass). carbon monoxide difference spectra of dithionite-reduced whole cells and crude ... | 2003 | 12801099 |
| analyses of microbial desulfurization reaction of alkylated dibenzothiophenes dissolved in oil phase. | the kinetics of the oil/water two-phase reaction system was analyzed, and the reaction was carried out with the desulfurization of alkylated dibenzothiophenes (cx-dbts) using the desulfurizing microorganism mycobacterium sp. g3. in the water-phase reaction system, the desulfurization activities were constant with respect to species of cx-dbts as substrates. however, the desulfurization activities in the oil/water two-phase reaction system against dbt, 4,6-dimethyl dbt, 4,6-diethyl dbt, 4,6-dipro ... | 2003 | 12800143 |
| prognosis and clinical evaluation of infection caused by rhodococcus equi in hiv-infected patients: a multicenter study of 67 cases. | to assess the clinical characteristics and the factors that influenced the prognosis of patients with hiv and infection caused by rhodococcus equi. | 2003 | 12796176 |
| emergence of rifampin-resistant rhodococcus equi with several types of mutations in the rpob gene among aids patients in northern thailand. | the antimicrobial susceptibilities of 30 rhodococcus equi isolates obtained from 30 patients between 1993 and 2001 in northern thailand were investigated. the mics showed a tendency toward resistance to various antibiotics but sensitivity to imipenem, minocycline, vancomycin, and teicoplanin (mics, </=0.5 micro g/ml) and relative sensitivity to meropenem, clarithromycin, and ciprofloxacin (mics, </=2 micro g/ml). of the 30 isolates, 26 were susceptible (mics, </=1 micro g/ml), 1 showed low-level ... | 2003 | 12791846 |
| cavitary pneumonia due to rhodococcus equi in a heart transplant recipient. | rhodococcus equi is an uncommon human pathogen that usually affects immunocompromised patients. we present a case of a 68-year-old male heart transplant recipient, who developed rhodococcal pneumonia with secondary bacteremia 10 months post-transplant. the patient was a retired carpenter who was involved in breeding of horses. he responded completely to the treatment with vancomycin and imipenem/cilastin, followed by oral ciprofloxacin and minocycline for total treatment duration of 5 months. th ... | 2003 | 12791074 |
| characterization of hydrocarbon-degrading microbial populations in contaminated and pristine alpine soils. | biodegradation of petroleum hydrocarbons in cold environments, including alpine soils, is a result of indigenous cold-adapted microorganisms able to degrade these contaminants. in the present study, the prevalence of seven genotypes involved in the degradation of n-alkanes (pseudomonas putida gpo1 alkb; acinetobacter spp. alkm; rhodococcus spp. alkb1, and rhodococcus spp. alkb2), aromatic hydrocarbons (p. putida xyle), and polycyclic aromatic hydrocarbons (p. putida ndob and mycobacterium sp. st ... | 2003 | 12788702 |
| relationship of demethylation processes to veratric acid concentration and cell density in cultures of rhodococcus erythropolis. | the aim of this study was to investigate the correlation between veratrate degradation, veratric acid concentration and cell density in rhodococcus erythropolis cultures. the optimum culture conditions for veratrate demethylation proved to be a cell density of a(660)=1 and a concentration of 0.02% veratrate. all the products of demethylation (i.e. vanillic and protocatechuic acids) were found to be present and correlated with the appearance of high levels of free radicals and formaldehyde after ... | 2003 | 12788048 |
| [unusual gram positive rods, causing pneumonia]. | in the last decade, a growing number of patients with pneumonia, caused by unusual gram positive rods have been observed. mostly, the patients had been infected as a consequence of impaired immunity. in some cases, bioterrorist activities may also induce pneumonia by gram positive rods (b. anthracis). in order to bring these organisms to the attention of the medical community, we present three clinical cases and describe six species of gram positive rods, known to provoke this kind of pneumonias ... | 2003 | 12784178 |
| analysis of virulence plasmid gene expression of intra-macrophage and in vitro grown rhodococcus equi atcc 33701. | rhodococcus equi is a soil organism that infects macrophages of foals and immunocompromised humans. virulence in foal isolates is tightly associated with an 80kb plasmid, which includes a pathogenicity island (pi) with a virulence-associated gene family, vap. a dna microarray containing 66 of 69 putative open reading frames (orfs) of the virulence plasmid was developed. virulence plasmid gene expression of r. equi grown in macrophages or under different conditions in vitro was compared against i ... | 2003 | 12781484 |
| partial genome sequencing of rhodococcus equi atcc 33701. | preliminary analysis of a partial (30% coverage) genome sequence of rhodococcus equi has revealed a number of important features. the most notable was the extent of the homology of genes identified with those of mycobacterium tuberculosis. the similarities in the proportion of genes devoted to fatty acid degradation and to lipid biosynthesis was a striking but not surprising finding given the relatedness of these organisms and their success as intracellular pathogens. the rapid recent improvemen ... | 2003 | 12781482 |
| a genetic system for the rapid isolation of aromatic-ring-hydroxylating dioxygenase activities. | aromatic-ring-hydroxylating dioxygenases (arhdos) are key enzymes in the aerobic bacterial metabolism of aromatic compounds. they are of biotechnological importance as they function as biocatalysts in the stereospecific synthesis of chiral synthons and the degradation of aromatic pollutants. this report describes the development and validation of a system for the rapid isolation and characterization of specific arhdo activities. the system is based on the identification of arhdo gene segments th ... | 2003 | 12777487 |
| structural and functional features of rhodococcus ruber lipoarabinomannan. | the genus rhodococcus is part of the phylogenetic group nocardioform actinomycetes, which also includes the genus mycobacterium: members of this phylogenetic group have a characteristic cell envelope structure, which is dominated by various complex lipids. among these, lipoglycans are of particular interest since mycobacterial lipoarabinomannans are important immunomodulatory molecules that are likely to be involved in the subsequent fate of mycobacterial bacilli once inside phagocytic cells. rh ... | 2003 | 12777484 |
| structure of rhodococcus erythropolis limonene-1,2-epoxide hydrolase reveals a novel active site. | epoxide hydrolases are essential for the processing of epoxide-containing compounds in detoxification or metabolism. the classic epoxide hydrolases have an alpha/beta hydrolase fold and act via a two-step reaction mechanism including an enzyme-substrate intermediate. we report here the structure of the limonene-1,2-epoxide hydrolase from rhodococcus erythropolis, solved using single-wavelength anomalous dispersion from a selenomethionine-substituted protein and refined at 1.2 a resolution. this ... | 2003 | 12773375 |
| quantitative structure-activity relationships for the enantioselectivity of oxirane ring-opening catalyzed by epoxide hydrolases. | the enantioselective ring-opening catalyzed by epoxide hydrolases originating from seven different sources of a series of 2,2-disubstituted oxiranes containing alkyl chains of different lengths, unsaturated (alkenyl, alkinyl) and aromatic groups as well as electronegative heteroatoms at various positions within the side chain was analyzed by quantitative structure-activity relationships. models for the enantioselectivity were derived with the aid of multiple linear regression analysis (mlr) usin ... | 2003 | 12767152 |
| lipoarabinomannans: from structure to biosynthesis. | mycobacterium tuberculosis, the causative agent of tuberculosis, is one of the most effective human pathogens and the molecular basis of its virulence remains poorly understood. here, we review our current knowledge about the structure and biosynthesis of the mycobacterial cell-wall lipoglycans, lipoarabinomannans (lam). lam are ubiquitous of mycobacteria and appear as the most potent non-peptidic molecules to modulate the host immune response. nevertheless, lam structure differs according to th ... | 2003 | 12765785 |
| host-vector system for phenol-degrading rhodococcus erythropolis based on corynebacterium plasmids. | the strain rhodococcus erythropolis ccm2595, which was shown to degrade phenol, was chosen for genetic studies. to facilitate strain improvement using the methods of gene manipulation, the technique of genetic transfer was introduced and cloning vectors were constructed. using the plasmid pfaj2574, an electrotransformation procedure yielding up to 7x10(4) transformants/microg dna was optimized. escherichia coli- r. erythropolis shuttle vectors were constructed using the replicons psr1 and pga1 f ... | 2003 | 12764568 |
| cloning and sequence analysis of the ces10 gene encoding a sphingomonas paucimobilis esterase. | the ces10 gene of the gellan gum-producing strain sphingomonas paucimobilis atcc 31461 was cloned and sequenced. multi-sequence alignment of the deduced protein indicated that ces10 belongs to the serine hydrolase family with a potential catalytic triad comprising ser(153) (within the g-x-s-x-g consensus sequence), his(75) and asp(125). the mixed block results obtained following pattern search and the low identities detected in a blast analysis indicate that ces10 is significantly different from ... | 2003 | 12764567 |
| nitrile biotransformations for highly efficient and enantioselective syntheses of electrophilic oxiranecarboxamides. | catalyzed by a nitrile hydratase/amidase-containing microbial rhodococcus sp. aj270 whole-cell catalyst, a number of racemic trans-2,3-epoxy-3-arylpropanenitriles 1 underwent rapid and efficient hydrolysis under very mild conditions to afford 2r,3s-2-arylglycidamides 2 in excellent yield with enantiomeric excess higher than 99.5%. the overall enantioselectivity of the biotransformations originated from the combined effects of a dominantly high 2s-enantioselective amidase and low 2s-enantioselect ... | 2003 | 12762774 |
| biotransformation of beta-ketosulfides to produce chiral beta-hydroxysulfoxides. | the biotransformations of a series of substituted phenylthio-2-propanone and benzylthio-2-propanone were carried out using helminthosporium sp. nrrl 4671, mortierella isabellina atcc 42613, or rhodococcus erythropolis igts8. several products gave microbial oxidation of sulfide to sulfoxide and reduction of carbonyl to secondary alcohol, producing beta-hydroxysulfoxides in medium to high enantiomeric and diastereomeric purities. fungal biotransformations using helminthosporium sp. and m. isabelli ... | 2003 | 12759809 |
| [the effect of sulfur on the hydrocarbon-oxidizing bacteria of different genera growth]. | 2003 | 12751253 | |
| [production of oil-processing compounds by microorganisms from the daqing oil field, china]. | twenty pure cultures isolated from formation waters of the daqing oil field were studied with respect to their capacity to produce surface-active compounds in media with individual hydrocarbons, lower alcohols, and fatty acids. aerobic saprotrophic bacteria belonging to the genera bacillus, brevibacillus, rhodococcus, dietzia, kocuria, gordonia, cellulomonas, clavibacter, pseudomonas, and acinetobacter decreased the surface tension of cultivation media from 55-63 to 28-44 mn/m. strains of bacill ... | 2003 | 12751245 |
| [fluorene cometabolism by rhodococcus rhodochrous and pseudomonas fluorescens]. | the transformation of fluorene by rhodococcus rhodochrous strain 172 grown on sucrose and pseudomonas fluorescens strain 26k grown on glycerol was studied as a function of the substrate concentration and the growth phase. under certain cultivation conditions, fluorene was completely consumed from the medium. the specific transformation rate of fluorene was considerably higher when it was transformed in the presence of the cosubstrates than when it served as the sole carbon source. an approach to ... | 2003 | 12751243 |
| 2-(2'-hydroxyphenyl)benzene sulfinate desulfinase from the thermophilic desulfurizing bacterium paenibacillus sp. strain a11-2: purification and characterization. | 2-(2'-hydroxyphenyl)benzene sulfinate (hpbsi) desulfinase (tdsb), which catalyzes the final step of desulfurization of dibenzothiophene (dbt), was purified from a thermophilic dbt- and benzothiophene (bt)-desulfurizing bacterium: paenibacillus sp. strain a11-2. the molecular mass of the purified enzyme was 31 kda and 39 kda by gel filtration and sodium dodecyl sulfate polyacrylamide gel electrophoresis, respectively, suggesting a monomeric structure. the optimal temperature and ph for the reacti ... | 2003 | 12743754 |
| macrokinetic and quantitative microbial investigation on a bench-scale biofilter treating styrene-polluted gaseous streams. | we performed a macrokinetic and quantitative microbial investigation of a continuously operating bench-scale biofilter treating styrene-polluted gases. the device was filled with a mixture of peat and glass beads as packing medium and inoculated with the styrene-oxidizing strain, rhodococcus rhodochrous al ncimb 13259. the experimental data of styrene and microbial concentrations, obtained at different biofilter heights, were used to evaluate the pollutant concentration profiles as well as the i ... | 2003 | 12740930 |
| culture collections and biochemistry. | this review describes the relationships and links between culture collections, which act as sources of genomes, transcriptomes, proteome, and metabolomes, and fields of research biochemistry that demand their support and help. in addition, the invaluable but not always rewarded efforts of these organizations as a source and conservator of organism diversity is discussed. biological waste-water treatment, ethanol as a non-finite source of energy, rhodococcus fascians as the source of a citrus-jui ... | 2003 | 12739105 |
| immunoglobulin g subisotype responses of pneumonic and healthy, exposed foals and adult horses to rhodococcus equi virulence-associated proteins. | rhodococcus equi causes severe pyogranulomatous pneumonia in foals and in immunocompromised humans. replication of virulent isolates within macrophages correlates with the presence of a large plasmid which encodes a family of seven virulence-associated proteins (vapa and vapc to vaph), whose functions are unknown. although cell-mediated immunity is thought to be crucial in eliminating r. equi infection, antibody partially protects foals. the antibody response to both vapa and vapc was similar in ... | 2003 | 12738629 |
| [rhodococcus equi pneumonia in aids: a case report] | we describe the first case, in campania, of rhodococcus equi pneumonia in an hiv positive subject. the clinical symptoms resolved after antibiotic therapy. the pulmonary lesion at 10 months of radiological follow up results just partially reduced in its diameters. | 1998 | 12732826 |
| purification and characterization of an inverting stereo- and enantioselective sec-alkylsulfatase from the gram-positive bacterium rhodococcus ruber dsm 44541. | whole cells of rhodococcus ruber dsm 44541 were found to hydrolyze (+/-)-2-octyl sulfate in a stereo- and enantiospecific fashion. when growing on a complex medium, the cells produced two sec-alkylsulfatases and (at least) one prim-alkylsulfatase in the absence of an inducer, such as a sec-alkyl sulfate or a sec-alcohol. from the crude cell-free lysate, two proteins responsible for sulfate ester hydrolysis (designated rs1 and rs2) were separated from each other based on their different hydrophob ... | 2003 | 12732552 |
| homologous npdgi genes in 2,4-dinitrophenol- and 4-nitrophenol-degrading rhodococcus spp. | rhodococcus (opacus) erythropolis hl pm-1 grows on 2,4,6-trinitrophenol or 2,4-dinitrophenol (2,4-dnp) as a sole nitrogen source. the nadph-dependent f(420) reductase (ndfr; encoded by npdg) and the hydride transferase ii (htii; encoded by npdi) of the strain were previously shown to convert both nitrophenols to their respective hydride meisenheimer complexes. in the present study, npdg and npdi were amplified from six 2,4-dnp degrading rhodococcus spp. the genes showed sequence similarities of ... | 2003 | 12732545 |
| cloning, sequence analysis, and heterologous expression of the gene encoding a (s)-specific alcohol dehydrogenase from rhodococcus erythropolis dsm 43297. | the gene encoding an (s)-specific nad-dependent alcohol dehydrogenase (re-adh) was isolated from the genomic dna of rhodococcus erythropolis dsm 43297. the nucleotide sequence of 1,047 bp, coding for 348 amino acids, was cloned in escherichia coli cells and successfully expressed. the subunit molecular mass as deduced from the amino acid sequence was determined to be 36.026 kda. the recombinant enzyme exhibited high thermostability, which facilitated its purification by heat treatment, followed ... | 2003 | 12719937 |
| in vitro susceptibilities of rhodococcus equi and other common equine pathogens to azithromycin, clarithromycin, and 20 other antimicrobials. | the objective of this study was to determine in vitro activities of azithromycin (azm), clarithromycin (clr), and 20 other antimicrobial agents against rhodococcus equi and other common equine bacterial pathogens. a total of 201 bacterial isolates from various equine clinical samples were examined. clr was more active than azm against r. equi, with mics at which 90% of the isolates were inhibited of 0.12 and 1.0 micro g/ml, respectively. other antimicrobial agents highly active against at least ... | 2003 | 12709351 |
| the cell wall of the pathogenic bacterium rhodococcus equi contains two channel-forming proteins with different properties. | we have identified in organic solvent extracts of whole cells of the gram-positive pathogen rhodococcus equi two channel-forming proteins with different and complementary properties. the isolated proteins were able to increase the specific conductance of artificial lipid bilayer membranes made from phosphatidylcholine-phosphatidylserine mixtures by the formation of channels able to be permeated by ions. the channel-forming protein pora(req) (r. equi pore a) is characterized by the formation of c ... | 2003 | 12700275 |
| substrate specificity and expression of three 2,3-dihydroxybiphenyl 1,2-dioxygenases from rhodococcus globerulus strain p6. | rhodococcus globerulus strain p6 contains at least three genes, bphc1, bphc2, and bphc3, coding for 2,3-dihydroxybiphenyl 1,2-dioxygenases; the latter two specify enzymes of the family of one-domain extradiol dioxygenases. in order to assess the importance of these different isoenzymes for the broad catabolic activity of this organism towards the degradation of polychlorinated biphenyls (pcbs), the capacities of recombinant enzymes expressed in escherichia coli to transform different chlorosubst ... | 2003 | 12700274 |
| heterologous expression of rhodococcus opacus l-amino acid oxidase in streptomyces lividans. | l-amino acid oxidase (l-aao) from rhodococcus opacus is a highly enantioselective enzyme with a broad substrate specificity that catalyses the oxidation of l-amino acids to keto acids. the lao-gene (ay053450) from r. opacus was cloned into different escherichia coli and streptomyces lividans expression vectors. expression in e. coli resulted in the accumulation of insoluble protein, but s. lividans was a suitable host for the heterologous production of l-aao. when using the thiostrepton-inducibl ... | 2003 | 12699695 |
| biodegradation of plasticizers by rhodococcus rhodochrous. | rhodococcus rhodochrous was grown in the presence of one of three plasticizers: bis 2-ethylhexyl adipate (beha), dioctyl phthalate (dop) or dioctyl terephthalate (dotp). none of the plasticizers were degraded unless another carbon source, such as hexadecane, was also present. when r. rhodochrous was grown with hexadecane as a co-substrate, beha was completely degraded and the dop was degraded slightly. about half of the dotp was degraded, if hexadecane were present. in all of these growth studie ... | 2002 | 12688586 |
| osteomyelitis of the pelvis caused by rhodococcus equi in a two-year-old horse. | a 2-year-old female quarter horse was evaluated for hind limb lameness, fever (40 degrees c [104 degrees f]), and lethargy of 2 weeks' duration. hypoproteinemia characterized by hypoalbuminemia and hyperfibrinogenemia was detected. abdominal ultrasonography revealed thickening of the right dorsal colon wall. treatment was instituted for putative right dorsal coliis. lameness evaluation localized signs of pain to the lumbar vertebrae or pelvis. radiography performed with the horse standing and nu ... | 2003 | 12685788 |
| the sequence and crystal structure of the alpha-amino acid ester hydrolase from xanthomonas citri define a new family of beta-lactam antibiotic acylases. | alpha-amino acid ester hydrolases (aehs) catalyze the hydrolysis and synthesis of esters and amides with an alpha-amino group. as such, they can synthesize beta-lactam antibiotics from acyl compounds and beta-lactam nuclei obtained from the hydrolysis of natural antibiotics. this article describes the gene sequence and the 1.9-a resolution crystal structure of the aeh from xanthomonas citri. the enzyme consists of an alpha/beta-hydrolase fold domain, a helical cap domain, and a jellyroll beta-do ... | 2003 | 12684501 |
| evaluation of a commercially available hyperimmune plasma product for prevention of naturally acquired pneumonia caused by rhodococcus equi in foals. | to determine efficacy of a commercially available hyperimmune plasma product for prevention of naturally acquired pneumonia caused by rhodococcus equi in foals. | 2002 | 12680449 |
| spatial and temporal analysis of the microbial community in slow sand filters used for treating horticultural irrigation water. | an experimental slow sand filter (ssf) was constructed to study the spatial and temporal structure of a bacterial community suppressive to an oomycete plant pathogen, phytophthora cryptogea. passage of water through the mature sand column resulted in complete removal of zoospores of the plant pathogen. to monitor global changes in the microbial community, bacterial and fungal numbers were estimated on selective media, direct viable counts of fungal spores were made, and the atp content was measu ... | 2003 | 12676691 |
| evaluation of white blood cell concentration, plasma fibrinogen concentration, and an agar gel immunodiffusion test for early identification of foals with rhodococcus equi pneumonia. | to evaluate wbc concentration, plasma fibrinogen concentration, and an agar gel immunodiffusion (agid) test for early identification of rhodococcus equi-infected foals. | 2003 | 12675301 |
| phenotypic mutants of the intracellular actinomycete rhodococcus equi created by in vivo himar1 transposon mutagenesis. | rhodococcus equi is a facultative intracellular opportunistic pathogen of immunocompromised people and a major cause of pneumonia in young horses. an effective live attenuated vaccine would be extremely useful in the prevention of r. equi disease in horses. toward that end, we have developed an efficient transposon mutagenesis system that makes use of a himar1 minitransposon delivered by a conditionally replicating plasmid for construction of r. equi mutants. we show that himar1 transposition in ... | 2003 | 12670990 |
| rhodococcus equi pneumonia in a heart transplant recipient in korea, with emphasis on microbial diagnosis. | rhodococcus equi is an opportunistic pathogen that usually causes infection in immunocompromised hosts. a heart transplant recipient who had been treated with amphotericin b for pulmonary aspergillosis showed newly developed multiple nodules with a central necrotic area in the right lower lobes. cultures of several blood samples and an aspirate of the lung nodule yielded a gram-positive coccobacillary bacterium, which was initially reported as a corynebacterium species, but was later identified ... | 2003 | 12667256 |
| crystal structure of a histidine-tagged serine hydrolase involved in the carbazole degradation (carc enzyme). | 2-hydroxy-6-oxo-6-(2(')-aminophenyl)-hexa-2,4-dienoate hydrolases (carc enzymes) from two carbazole-degrading bacteria were purified using recombinant escherichia coli strains with the histidine (his)-tagged purification system. the his-tagged carc (ht-carc) enzymes from pseudomonas resinovorans strain ca10 (ht-carc(ca10)) and janthinobacterium sp. strain j3 (ht-carc(j3)) exhibited hydrolase activity toward 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate as the purified native carc(ca10) did. ht-carc( ... | 2003 | 12659866 |
| [laryngeal infection by rhodococcus equi in patient with aids]. | the rhodococcus equi is an aerobic gram positive pleomorphic bacillus, that was isolated for the first time like a producer of bronchopneumonia in young horses. every time more often, it is being recognized as a pathogen in humans, mainly in the immunodepressed population. we described a case, until now exceptional, of laryngeal infection by rhodococcus equi in a patient with positive serology for the virus of the human immunodeficiency (hiv), and we reviewed some clinical and epidemiological ch ... | 2002 | 12658846 |
| shifts in diversity and microscale distribution of the adapted bacterial phenotypes due to hg(ii) spiking in soil. | in a previous experiment [ranjard et al. (2000) fems microbiol ecol 31:107-115], the spatial heterogeneity of a mercury impact on soil bacterial community was revealed by an increase of mercury-resistant (hgr) bacterial numbers in the outer fraction and the sand fractions when compared to those in the silt fractions. the objectives of the present study were (i) to investigate whether mercury exposure affects the diversity and the distribution within the various fractions of the hgr populations a ... | 2003 | 12658524 |
| pathogenic nocardia, rhodococcus, and related organisms are highly susceptible to imidazole antifungals. | rhodococcus equi and species of nocardia and gordonia may be human opportunistic pathogens. we find that these, as well as several isolates from closely related genera, are highly susceptible to the imidazoles bifonazole, clotrimazole, econazole, and miconazole, whose mics are </=1 micro g/ml. in liquid cultures 1 micro g of the drug/ml was bacteriostatic and 10 micro g/ml was bactericidal. on solid media at 10 micro g of azole/ml no resistant mutants could be isolated. an mic of 1 to 15 micro g ... | 2003 | 12654698 |
| activities of abt-773 against listeria monocytogenes and coryneform bacteria of clinical interest. | the in vitro activities of abt-773 were evaluated against 15 listeria monocytogenes strains and 196 coryneform bacteria isolated from clinical samples. one hundred percent of the l. monocytogenes strains were inhibited by </=0.015 micro g of abt-773/ml. mics of abt-773 ( micro g/ml) at which 50% of the isolates tested were inhibited (mic(50)s) and mic(90)s for other organisms were 0.125 and 0.5 (corynebacterium amycolatum), 1 and >32 (corynebacterium jeikeium), 0.03 and >32 (corynebacterium minu ... | 2003 | 12654678 |
| the use of chemical and molecular microbial indicators for faecal source identification. | identifying the source of faecal pollution is important to enable appropriate management of faecal pollution of water. we are developing and evaluating a combination of these microbial and chemical indicators better able to identify the source of faecal pollution. these assays make use of a combination of direct pcr, culturing, and colony hybridisation to identify source specific species of bifidobacterium, rhodococcus and bacteroides. in conjunction with assays for (a) fluorescent whitening age ... | 2003 | 12639003 |
| practical synthesis of optically active alpha,alpha-disubstituted malonamic acids through asymmetric hydrolysis of malonamide derivatives with rhodococcus sp. cgmcc 0497. | a variety of alpha,alpha-disubstituted malonamides undergo enantioselective hydrolysis with rhodococcus sp. cgmcc 0497 to give challenging enantiopure alpha,alpha-disubstituted malonamic acids with up to >99% enantiomeric excesses and 98% chemical yields. the enantioselectivity originated from the effects of a highly enantioselective amidase. the products could be converted to valuable (r)- or (s)-alpha,alpha-dialkylated amino acids after routine conversions. | 2003 | 12636421 |
| performance of five serological assays for diagnosis of rhodococcus equi pneumonia in foals. | the performance of four enzyme-linked immunosorbent assays (elisas) (elisa-6939, elisa-33701, elisa-vapa, and elisa-california) and an agar gel immunodiffusion test for diagnosis of rhodococcus equi pneumonia in foals was evaluated. antibody concentrations of foals with culture-confirmed r. equi pneumonia (n = 41) were compared to those of age-matched pasturemates that remained clinically healthy during the entire breeding season (n = 24). for each serological assay evaluated, selection of a low ... | 2003 | 12626449 |
| clearance of virulent but not avirulent rhodococcus equi from the lungs of adult horses is associated with intracytoplasmic gamma interferon production by cd4+ and cd8+ t lymphocytes. | rhodococcus equi is a gram-positive bacterium that infects alveolar macrophages and causes rhodococcal pneumonia in horses and humans. the virulence plasmid of r. equi appears to be required for both pathogenicity in the horse and the induction of protective immunity. an understanding of the mechanisms by which virulent r. equi circumvents protective host responses and by which bacteria are ultimately cleared is important for development of an effective vaccine. six adult horses were challenged ... | 2003 | 12626444 |
| [effects of nitriles and amides on the growth and the nitrile hydratase activity of the rhodococcus sp. strain gt1]. | effects of some nitriles and amides, as well as glucose and ammonium, on the growth and the nitrile hydratase (ec 4.2.1.84) activity of the rhodococcus sp. strain gt1 isolated from soil were studied. the activity of nitrile hydratase mainly depended on carbon and nitrogen supply to cells. the activity of nitrile hydratase was high in the presence of glucose and ammonium at medium concentrations and decreased at concentrations of glucose more than 0.3%. saturated unsubstituted aliphatic nitriles ... | 2003 | 12625044 |
| synthesis of imidazol-2-yl amino acids by using cells from alkane-oxidizing bacteria. | sixty-one strains of alkane-oxidizing bacteria were tested for their ability to oxidize n-(2-hexylamino-4-phenylimidazol-1-yl)-acetamide to imidazol-2-yl amino acids applicable for pharmaceutical purposes. after growth with n-alkane, 15 strains formed different imidazol-2-yl amino acids identified by chemical structure analysis (mass and nuclear magnetic resonance spectrometry). high yields of imidazol-2-yl amino acids were produced by the strains gordonia rubropertincta sbug 105, gordonia terra ... | 2003 | 12620858 |
| biotransformation of hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) by a rabbit liver cytochrome p450: insight into the mechanism of rdx biodegradation by rhodococcus sp. strain dn22. | a unique metabolite with a molecular mass of 119 da (c(2)h(5)n(3)o(3)) accumulated during biotransformation of hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) by rhodococcus sp. strain dn22 (d. fournier, a. halasz, j. c. spain, p. fiurasek, and j. hawari, appl. environ. microbiol. 68:166-172, 2002). the structure of the molecule and the reactions that led to its synthesis were not known. in the present study, we produced and purified the unknown metabolite by biotransformation of rdx with rhodococ ... | 2003 | 12620815 |
| molecular cloning and characterization of an amidase from arabidopsis thaliana capable of converting indole-3-acetamide into the plant growth hormone, indole-3-acetic acid. | acylamidohydrolases from higher plants have not been characterized or cloned so far. atami1 is the first member of this enzyme family from a higher plant and was identified in the genome of arabidopsis thaliana based on sequence homology with the catalytic-domain sequence of bacterial acylamidohydrolases, particularly those that exhibit indole-3-acetamide amidohydrolase activity. atami1 polypeptide and mrna are present in leaf tissues, as shown by immunoblotting and rt-pcr, respectively. atami1 ... | 2003 | 12620340 |
| qcrcab operon of a nocardia-form actinomycete rhodococcus rhodochrous encodes cytochrome reductase complex with diheme cytochrome cc subunit. | structural genes encoding quinol-cytochrome c reductase (qcr) were cloned and sequenced from nocardia-form actinomycete rhodococcus rhodochrous. qcrc and qcra encode diheme cytochrome cc and the rieske fe-s protein, respectively, while the qcrb product is a diheme cytochrome b. these amino acid sequences are similar to those of corynebacterium and mycobacterium, the members of high g+c content firmicutes. the presence of diheme cytochrome cc subunit as a sole c-type cytochrome in these organisms ... | 2003 | 12615356 |
| highly enantioselective synthesis of alpha,alpha-disubstituted malonamic acids through asymmetric hydrolysis of dinitriles with rhodococcus sp. cgmcc 0497. | highly enantioselective hydrolysis of alpha,alpha-disubstituted malononitriles by the strain rhodococcus sp. cgmcc 0497 expressing both nitrile hydratase and amidase activity to give (r)-alpha,alpha-disubstituted malonamic acids which could be converted to valuable (r)- or (s)-alpha-alkylated amino acids are reported and the yields of the products are improved remarkably at a lower reaction temperature. | 2003 | 12613625 |
| biodegradation of bis(1-chloro-2-propyl) ether via initial ether scission and subsequent dehalogenation by rhodococcus sp. strain dtb. | rhodococcus sp. strain dtb (dsm 44534) grows on bis(1-chloro-2-propyl) ether (dde) as sole source of carbon and energy. the non-chlorinated diisopropyl ether and bis(1-hydroxy-2-propyl) ether, however, did not serve as substrates. in ether degradation experiments with dense cell suspensions, 1-chloro-2-propanol and chloroacetone were formed, which indicated that scission of the ether bond is the first step while dehalogenation of the chlorinated c(3)-compounds occurs at a later stage of the degr ... | 2003 | 12605291 |
| a serological survey of rhodococcus equi infection in foals in central italy: comparison of two antigens using an elisa test. | a serological survey of rhodococcus equi infection was carried out on 602 blood samples collected from foals in central italy. the assay was performed with an elisa test using two different antigens prepared with reference strains of r. equi, atcc 33071 and atcc 6939. a positive reaction was obtained on 81 serum samples (13.45%) (od > or = 0.3) using antigen atcc 33071, and on 73 serum samples (12.12%) using antigen atcc 6939. although the frequency of the disease was not high, the serological p ... | 2003 | 12602683 |
| epidemiologic study of results of pulsed-field gel electrophoresis of isolates of rhodococcus equi obtained from horses and horse farms. | to compare isolates of rhodococcus equi on the basis of geographic source and virulence status by use of pulsed-field gel electrophoresis (pfge). | 2003 | 12602583 |
| evaluation of equine breeding farm management and preventative health practices as risk factors for development of rhodococcus equi pneumonia in foals. | to determine whether foal management practices, environmental management, and preventative health practices are risk factors for development of rhodococcus equi pneumonia in foals. | 2003 | 12597421 |
| evaluation of equine breeding farm characteristics as risk factors for development of rhodococcus equi pneumonia in foals. | to identify farm characteristics as risk factors for the development of rhodococcus equi pneumonia in foals. | 2003 | 12597420 |
| cloning of baeyer-villiger monooxygenases from comamonas, xanthobacter and rhodococcus using polymerase chain reaction with highly degenerate primers. | to clone novel type 1 baeyer-villiger monooxygenase (bvmo) genes, we isolated or collected 25 bacterial strains able to grow on alicyclic compounds. twelve of the bacterial strains yielded polymerase chain reaction (pcr) fragments with highly degenerate primers based on the sequences of known and putative bvmos. all these fragments were found to encode peptides homologous to published bvmo sequences. the complete bvmo genes and flanking dna were cloned from a comamonas, a xanthobacter and a rhod ... | 2003 | 12588297 |
| desulfonation and degradation of the disulfodiphenylethercarboxylates from linear alkyldiphenyletherdisulfonate surfactants. | earlier work showed that the biodegradation of a commercial linear monoalkyldiphenyletherdisulfonate surfactant as a carbon source for microbial growth leads to the quantitative formation of corresponding disulfodiphenylether carboxylates (dsdpecs), which were not degraded. alpha-proteobacterium strain ds-1 (dsm 13023) catalyzes these reactions. these dsdpecs have now been characterized by high-pressure liquid chromatography coupled via an electrospray interface to a mass spectrometer. dsdpecs w ... | 2003 | 12571015 |
| a simple method to observe organic solvent drops with a standard optical microscope. | 2003 | 12567404 | |
| [image of the month. pulmonary malacoplakia due to rhodococcus equi in a patient with aids]. | 2002 | 12564097 | |
| characterization of extradiol dioxygenases from a polychlorinated biphenyl-degrading strain that possess higher specificities for chlorinated metabolites. | recent studies demonstrated that 2,3-dihydroxybiphenyl 1,2-dioxygenase from burkholderia sp. strain lb400 (dhbdlb400; ec 1.13.11.39) cleaves chlorinated 2,3-dihydroxybiphenyls (dhbs) less specifically than unchlorinated dhb and is competitively inhibited by 2',6'-dichloro-2,3-dihydroxybiphenyl (2',6'-dicl dhb). to determine whether these are general characteristics of dhbds, we characterized dhbdp6-i and dhbdp6-iii, two evolutionarily divergent isozymes from rhodococcus globerulus strain p6, ano ... | 2003 | 12562795 |
| pentacyclic triterpenoids from embelia schimperi. | five oleanane-type pentacyclic triterpenoids were isolated by chromatographic separation of a chloroform extract of the stem bark of embelia schimperi. three of these compounds have a methyleneoxy bridge. two compounds, embelinone and schimperinone, are reported here for the first time from a natural source (they have been synthesized previously during chemical transformations). their structures were determined by spectroscopic techniques, among which 2-d nmr was useful for complete characteriza ... | 2003 | 12560028 |
| [study on the reclassification of two deposited strains as4.1186 and as4.1187 of nocardia]. | polyphasic evidence for the reclassification of nocardia nostocoides as4.1186 and nocardia salmonicolor var. aurantiaca as4.1187 indicated that strain as4.1186 was closely related to saccharothrix texasensis and strain as4.1187 was very closely related to rhodococcus ruber. binary 16s rdna sequence for the pair as4.1186 and saccharothrix texasensis nrrl b-16134t, and the pair as4.1187 and rhodococcus ruber dsm 43338t exhibited 99.3% and 99.5% similarity, respectively. corresponding dna-dna reass ... | 2002 | 12557341 |
| on the organic solvent and thermostability of the biocatalytic redox system of rhodococcus ruber dsm 44541. | the sec-alcohol dehydrogenase activity of whole cells of rhodococcus ruber dsm 44541 has been employed as an efficient biocatalytic redox system due to the use of acetone and 2-propanol at elevated concentrations for cofactor regeneration in the oxidation and reduction mode, respectively, and external addition of nadh/nad(+) can be omitted. the operational half-life time of the redox system is 29 hours in 20% v/v acetone and 37 hours in 30% v/v 2-propanol. the redox system allows the enantiosele ... | 2003 | 12557320 |
| cavitary rhodococcus equi pneumonia with endobronchial granulomas: report of an unusual case. | an unusual case of cavitary rhodococcus equi pneumonia with endobronchial granulomas in congenital hiv infection is presented. the clinical features and radiological manifestations of pulmonary r. equi infection are discussed. | 2003 | 12557073 |
| [progress of biodesulfurization]. | 2001 | 12552818 | |
| a rapid and sensitive fluorometric assay method for the determination of nitrilase activity. | a rapid, simple and sensitive fluorometric assay method for the determination of nitrilase activity is described. 3-cyanopyridine was hydrolysed to nicotinic acid by rhodococcus rhodochrous and the liberated nh(3) was allowed to react with buffered o -phthaldialdehyde-2-mercaptoethanol solution (ph 7.4) to form a fluorochrome. the fluorescence intensity was found to be stable after 20 min incubation at room temperature, and the optimum ph for the reaction was found to be 7.4. the fluorescence in ... | 2003 | 12549980 |
| clostridium difficile infections in animals with special reference to the horse. a review. | in human medicine, clostridium (c.) difficile is since many years a well-known cause of nosocomial diarrhea induced by antibiotic treatment. in horses, c. difficile was recently suggested as a possible enteric pathogen. the bacterium is associated with acute colitis in mature horses following treatment with antibiotics. c. difficile, and/or its cytotoxin, is also associated with acute colitis in mares when their foals are being treated with erythromycin and rifampicin for rhodococcus equi pneumo ... | 2002 | 12540137 |