Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| whole-genome mapping of 5' rna ends in bacteria by tagged sequencing: a comprehensive view in enterococcus faecalis. | enterococcus faecalis is the third cause of nosocomial infections. to obtain the first snapshot of transcriptional organizations in this bacterium, we used a modified rna-seq approach enabling to discriminate primary from processed 5' rna ends. we also validated our approach by confirming known features in escherichia coli. we mapped 559 transcription start sites (tsss) and 352 processing sites (psss) in e. faecalis. a blind motif search retrieved canonical features of siga- and sign-dependent p ... | 2015 | 25737579 |
| [case of a 14-year-old boy with chronic tubulointerstitial nephritis first diagnosed as acute focal bacterial nephritis]. | a 14-year-old boy was admitted to a general hospital because of prolonged fever of unknown origin. after enterococcus feacalis was detected from his urine and abdominal contrast enhanced computed tomography and 99m-tc dimercaptosuccinic acid scintigram showed multiple focal defects, he was diagnosed as acute focal bacterial nephritis (afbn). his condition recovered as a result of ampicillin (abpc)and cefotaxime infusion. there was no specific finding in voiding cystography. six months later, his ... | 2015 | 25735087 |
| the fsr quorum-sensing system and cognate gelatinase orchestrate the expression and processing of proprotein ef_1097 into the mature antimicrobial peptide enterocin o16. | a novel antimicrobial peptide designated enterocin o16 was purified from enterococcus faecalis. mass spectrometry showed a monoisotopic mass of 7,231 da, and n-terminal edman degradation identified a 29-amino-acid sequence corresponding to residues 90 to 119 of the ef_1097 protein. bioinformatic analysis showed that enterocin o16 is composed of the 68 most c-terminal residues of the ef_1097 protein. introduction of an in-frame isogenic deletion in the ef1097 gene abolished the production of ente ... | 2015 | 25733609 |
| ec300: a phage-based, bacteriolysin-like protein with enhanced antibacterial activity against enterococcus faecalis. | bacteriophage lytic enzymes, either endolysins or virion-associated lysins, have been receiving considerable attention as potential antibacterial agents, particularly for the combat of antibiotic-resistant gram-positive pathogens. a conclusion that easily emerges from the careful analysis of a great number of reports on the field is that the activity of phage lytic enzymes is rarely studied in conditions that support robust growth of the target bacteria. here, we report the construction and stud ... | 2015 | 25728445 |
| the sfani restriction-modification system from enterococcus faecalis neb215 is located on a putative mobile genetic element. | a type iis restriction-modification (r-m) system sfani from enterococcus faecalis neb215 has been characterized. the sfanim gene was cloned by the methylase selection method. methyltransferase sfani, a protein of 695 amino acids, consists of two domains responsible for different dna-strand recognition and modification, and a putative dna-binding hth domain located in the n-terminal part of the protein. the sfanir gene, located adjacent to the gene of the cognate modification methyltransferases, ... | 2015 | 25724535 |
| chronic bacterial seminal vesiculitis as a potential disease entity in men with chronic prostatitis. | to investigate bacterial infection in the seminal vesicles by bacteriological examination and radionuclide imaging in men with chronic prostatitis. | 2015 | 25721352 |
| validation of a novel rinse and filtration method for efficient processing of fresh produce samples for microbiological indicator enumeration. | several methods have been described to prepare fresh produce samples for microbiological analysis, each with its own advantages and disadvantages. the aim of this study was to compare the performance of a novel combined rinse and membrane filtration method to two alternative sample preparation methods for the quantification of indicator microorganisms from fresh produce. decontaminated cantaloupe melons and jalapeño peppers were surface inoculated with a cocktail containing 10(6) cfu/ml escheric ... | 2015 | 25719876 |
| combined effect of a mixture of tetracycline, acid, and detergent, and nisin against enterococcus faecalis and actinomyces viscosus biofilms. | to evaluate the combined effect of a mixture of tetracycline, acid, and detergent (mtad) and nisin against enterococcus faecalis (e. faecalis) and actinomyces viscosus (a. viscosus) biofilms. | 2015 | 25719587 |
| antibacterial and smear layer removal capability of oregano extract solution. | the aim of this in vitro study was to evaluate the antimicrobial effect of oregano extract solution (oes) against enterococcus faecalis within root canals and dentin tubules, and its effect on smear layer. | 2017 | 25713479 |
| in vitro activity of gentamicin, vancomycin or amikacin combined with edta or l-arginine as lock therapy against a wide spectrum of biofilm-forming clinical strains isolated from catheter-related infections. | treatment of catheter-related bloodstream infections (crbsi) is hampered by the characteristic tolerance of bacterial biofilms towards antibiotics. our objective was to study the effect of the combination of antibiotics and the alkaline amino acid l-arginine or the cation chelator edta on the bacterial killing of in vitro biofilms formed by an array of clinical strains responsible for crbsi and representative of epidemiologically relevant bacterial species. | 2015 | 25712314 |
| spread of an enterococcus faecalis sequence type 6 (cc2) clone in patients undergoing selective decontamination of the digestive tract. | enterococcus faecalis (e. faecalis) is a common cause of nosocomial infection in immunocompromised patients. the presence and dissemination of high-risk clonal complexes, such as cc2, is an ongoing problem in hospitals. the aim of this work was to characterize 24 e. faecalis isolates from icu patients undergoing selective decontamination of the digestive tract (sdd) by phenotypical (antimicrobial susceptibility) and genotypical (presence of virulence genes, rapd-pcr and mlst) methods. our result ... | 2015 | 25712203 |
| in vitro antimicrobial and cytotoxic effects of anacardium occidentale and mangifera indica in oral care. | oral health is an integral and important component of general health. infectious diseases such as caries, periodontal, and gingivitis indicate the onset of imbalance in homeostasis between oral micro biota and host. the present day medicaments used in oral health care have numerous side effects. the uses of herbal plants as an alternative have gained popularity due to side effects of antibiotics and emergence of multidrug resistant strains. anacardium occidentale (cashew) and mangifera indica (m ... | 2017 | 25709341 |
| antimicrobial activity of root canal irrigants associated with cetrimide against biofilm and planktonic enterococcus faecalis. | to evaluate the antibacterial activity of sodium hypochlorite (naocl) and chlorhexidine (chx) alone or associated with cetrimide (ctr), and qmix against biofilm and planktonic enterococcus faecalis (e. faecalis) [american type culture collection (atcc) 29212]. | 2014 | 25707833 |
| phage-mediated dispersal of biofilm and distribution of bacterial virulence genes is induced by quorum sensing. | the microbiome and the phage meta-genome within the human gut are influenced by antibiotic treatments. identifying a novel mechanism, here we demonstrate that bacteria use the universal communication molecule ai-2 to induce virulence genes and transfer them via phage release. high concentrations (i.e. 100 μm) of ai-2 promote dispersal of bacteria from already established biofilms, and is associated with release of phages capable of infecting other bacteria. enterococcus faecalis v583δabc harbour ... | 2015 | 25706310 |
| is it safe to reuse dental implant healing abutments sterilized and serviced by dealers of dental implant manufacturers? an in vitro sterility analysis. | this study was undertaken to investigate the sterility of used healing abutments sterilized and serviced by dealers of dental implant manufacturers. | 2015 | 25706262 |
| vancomycin-resistant enteroccus faecium and enterococcus faecalis isolated from education hospital of iran. | enterococci are opportunistic pathogens which represent one of the leading agents of nosocomial infections, especially urinary tract infections (uti) in hospitalized patients. the aim of the present study was to determine the resistance pattern and the type of resistance genes in vancomycin-resistant enterococcus isolated from an educational hospital in iran. | 2014 | 25705299 |
| novel coating of surgical suture confers antimicrobial activity against porphyromonas gingivalis and enterococcus faecalis. | the oral cavity is colonized by >10(9) bacteria, many of which can increase heart disease risk when seeded into the bloodstream. most dentoalveolar surgeries require the use of surgical sutures. suture placement and removal can increase the risk of postoperative infection and bacteremia. the aim of this study is to evaluate the antimicrobial activity of a novel quaternary ammonium compound, k21, when coated on different suture materials. | 2015 | 25703732 |
| ultrasonic activation and chemical modification of photosensitizers enhances the effects of photodynamic therapy against enterococcus faecalis root-canal isolates. | the aim of this study was to evaluate the effect of photodynamic therapy (pdt) on enterococcus faecalis biofilms in artificially infected root canals using modified photosensitizers and passive ultrasonic activation. | 2015 | 25703104 |
| biofilms of enterococcus faecalis and enterococcus faecium isolated from the processing of ricotta and the control of these pathogens through cleaning and sanitization procedures. | the biofilm formation of enterococcus faecalis and enterococcus faecium isolated from the processing of ricotta on stainless steel coupons was evaluated, and the effect of cleaning and sanitization procedures in the control of these biofilms was determined. the formation of biofilms was observed while varying the incubation temperature (7, 25 and 39°c) and time (0, 1, 2, 4, 6 and 8 days). at 7°c, the counts of e. faecalis and e. faecium were below 2 log10 cfu/cm(2). for the temperatures of 25 an ... | 2015 | 25702883 |
| effects of ciprofloxacin-containing scaffolds on enterococcus faecalis biofilms. | antibiotic-containing polymer-based nanofibers (hereafter referred to as scaffolds) have demonstrated great potential for their use in regenerative endodontics from both an antimicrobial and cytocompatibility perspective. this study sought to evaluate in vitro the effects of ciprofloxacin (cip)-containing polymer scaffolds against enterococcus faecalis biofilms. | 2015 | 25698261 |
| [inferior vena cava thrombosis and endocarditis in a premature patient: case report]. | thrombosis of the inferior vena cava represents a significant percentage of all venous thrombosis that take place during the neonatal period, generally associated with risk factors such as the use of central venous catheter. the incidence of bacterial endocarditis in preterm infants is low. | 2014 | 25697617 |
| nonselective mevalonate kinase inhibitor as a novel class of antibacterial agents. | introduction. there are a few evidences about targeting isoprenoids biosynthesis pathway in bacteria for finding new antibiotics. this study was conducted to assess antibacterial effects of vanadyl sulfate (vs), one of the mevalonate kinase inhibitors to find a new target for killing bacteria. materials and methods. antibacterial effect of vs alone and in combination with glycine or edta was assessed on escherichia coli and pseudomonas aeruginosa as gram-negative and staphylococcus aureus and en ... | 2015 | 25692035 |
| the effect of diluted triple and double antibiotic pastes on dental pulp stem cells and established enterococcus faecalis biofilm. | to investigate the effect of various dilutions of antibiotic medicaments used in endodontic regeneration on the survival of human dental pulp stem cells (dpscs) and to determine their antibacterial effect against established enterococcus faecalis biofilm. | 2015 | 25689981 |
| orosomucoid 1 drives opportunistic infections through the polarization of monocytes to the m2b phenotype. | orosomucoid (orm, composed of two isoforms, orm1 and orm2) has been described as an inducer of m2 macrophages, which are cells that decrease host antibacterial innate immunities. however, it is unknown which phenotypes of m2 macrophages are induced by orm. in this study, healthy donor monocytes stimulated with orm (orm-monocytes) were characterized phenotypically and biologically. ccl1 (a biomarker of m2b macrophages) and il-10 were detected in monocyte cultures supplemented with orm1; however, ... | 2015 | 25689617 |
| growth-dependent photoinactivation kinetics of enterococcus faecalis. | to investigate how the growth stage of enterococcus faecalis affects its photoinactivation in clear water. | 2015 | 25688992 |
| the length of a lantibiotic hinge region has profound influence on antimicrobial activity and host specificity. | lantibiotics are ribosomally synthesized (methyl)lanthionine containing peptides which can efficiently inhibit the growth of gram-positive bacteria. as lantibiotics kill bacteria efficiently and resistance to them is difficult to be obtained, they have the potential to be used in many applications, e.g., in pharmaceutical industry or food industry. nisin can inhibit the growth of gram-positive bacteria by binding to lipid ii and by making pores in their membrane. the c-terminal part of nisin is ... | 2015 | 25688235 |
| [synergistic effects of lysozyme with edta-2na on antibacterial activity]. | to evaluate the synergistic antibacterial effects of lysozyme with ethylenediaminetetraacetic acid disodium salt (edta-2na) on enterococcus faecalis (e. faecalis) and porphyromonas endodontalis (p. endodontalis). | 2015 | 25686329 |
| bactericidal efficacy of elevated ph on fish pathogenic and environmental bacteria. | ship ballast water is a recognized medium for transfer and introductions of nonindigenous species. there is a need for new ballast water treatment methods that effectively and safely eliminate or greatly minimize movements of these species. the present study employed laboratory methods to evaluate the bactericidal efficacy of increased ph (ph 10.0-12.0) for exposure durations of up to 72 h to kill a variety of gram-negative and gram-positive bacteria including fish pathogens (aeromonas spp., yer ... | 2012 | 25685439 |
| tridentate hydrazone metal complexes derived from cephalexin and 2-hydrazinopyridine: synthesis, characterization and antibacterial activity. | metal(ii) coordination compounds of a tridentate hydrazone ligand (hl) derived from the condensation of cephalexin antibiotic with 2-hydrazinopyridine were synthesized. the hydrazone ligand and mononuclear [ml(oac)(h2o)] (m(ii)=mn, co, ni, cu, zn, ag) complexes were characterized by several techniques, including elemental and thermal analysis, molar conductance and magnetic susceptibility measurements, electronic, ft-ir, epr and (1)h nmr spectral studies. the cephalexin 2-pyridinylhydrazone liga ... | 2015 | 25677531 |
| antimicrobial and cytotoxic activity of ferula gummosa plant essential oil compared to naocl and chx: a preliminary in vitro study. | the usage of medicinal plants as natural antimicrobial agents has grown in many fields including dental medicine. the aim of this in vitro study was three-fold: (i) to determine the chemical compositions of the ferula gummosa essential oil (fgeo), (ii) to compare the antimicrobial efficacy of the oil with sodium hypochlorite (naocl) and chlorhexidine (chx), (iii) to assess the toxic behavior of fgeo in different concentrations compared to 5% naocl and 0.2% chx. | 2015 | 25671213 |
| antimicrobial and antifungal activity of pelargonium roseum essential oils. | the antiseptic qualities of aromatic and medicinal plants and their extracts have been recognized since antiquity, while attempts to characterize these properties in the laboratory date back the beginning of the xx(th) century. in the current study essential oils obtained from pelargonium roseum (geraniacea) were analyzed for their antibacterial and antifungal activities. | 2014 | 25671182 |
| antimicrobial peptides expressed in medicinal maggots of the blow fly lucilia sericata show combinatorial activity against bacteria. | the larvae of the common green bottle fly (lucilia sericata) produce antibacterial secretions that have a therapeutic effect on chronic and nonhealing wounds. recent developments in insect biotechnology have made it possible to use these larvae as a source of novel anti-infectives. here, we report the application of next-generation rna sequencing (rna-seq) to characterize the transcriptomes of the larval glands, crop, and gut, which contribute to the synthesis of antimicrobial peptides (amps) an ... | 2015 | 25666157 |
| probiotic potential of lactobacillus strains isolated from sorghum-based traditional fermented food. | sorghum-based traditional fermented food was screened for potential probiotic lactic acid bacteria. the isolates were identified by biochemical, physiological and genetic methods. species identification was done by 16s rrna sequence analysis. the functional probiotic potential of the two lactobacillus species viz., lactobacillus plantarum (lact. plantarum) and lactobacillus pentosus (lact. pentosus) was assessed by different standard parameters. the strains were tolerant to ph 2 for 1 h and resi ... | 2015 | 25666113 |
| targeting enterococcus faecalis biofilms with phage therapy. | phage therapy has been proven to be more effective, in some cases, than conventional antibiotics, especially regarding multidrug-resistant biofilm infections. the objective here was to isolate an anti-enterococcus faecalis bacteriophage and to evaluate its efficacy against planktonic and biofilm cultures. e. faecalis is an important pathogen found in many infections, including endocarditis and persistent infections associated with root canal treatment failure. the difficulty in e. faecalis treat ... | 2015 | 25662974 |
| bioluminescence based biosensors for quantitative detection of enterococcal peptide-pheromone activity reveal inter-strain telesensing in vivo during polymicrobial systemic infection. | enterococcus faecalis is a significant threat in the nosocomial setting due to the emergence of isolates that are multi-antibiotic resistant, refractory to the available therapies and equipped with a variety of pathogenicity determinants. this bacterium uses quorum-sensing systems to regulate its physiological processes, including the expression of virulence traits, to adapt and proliferate within a host. here, we describe the construction and application of two bioluminescence-based reporter sy ... | 2015 | 25661457 |
| bactericidal activity and mechanism of photoirradiated polyphenols against gram-positive and -negative bacteria. | the bactericidal effect of various types of photoirradiated polyphenols against gram-positive and -negative bacteria was evaluated in relation to the mode of action. gram-positive bacteria (enterococcus faecalis, staphylococcus aureus, and streptococcus mutans) and gram-negative bacteria (aggregatibacter actinomycetemcomitans, escherichia coli, and pseudomonas aeruginosa) suspended in a 1 mg/ml polyphenol aqueous solution (caffeic acid, gallic acid, chlorogenic acid, epigallocatechin, epigalloca ... | 2015 | 25660393 |
| utilization of blood cultures in danish hospitals: a population-based descriptive analysis. | this national population-based study was conducted as part of the development of a national automated surveillance system for hospital-acquired bacteraemia and ascertains the utilization of blood cultures (bcs). a primary objective was to understand how local differences may affect interpretation of nationwide surveillance for bacteraemia. from the danish microbiology database, we retrieved all bcs taken between 2010 and 2013 and linked these to admission data from the national patient registry. ... | 2015 | 25658520 |
| daptomycin versus vancomycin in an enterococcus faecalis endophthalmitis rabbit model. | to compare bactericidal activities of daptomycin (dap) and vancomycin (van) in an experimental rabbit model of enterococcus faecalis endophthalmitis. | 2016 | 25658242 |
| drinking water quality and public health in southwestern saudi arabia: the need for a national monitoring program. | the aim was to investigate the bacteriological quality of drinking water, and explore the factors involved in the knowledge of the public about the quality of drinking water in najran region, saudi arabia. | 2017 | 25657607 |
| sealing ability of a novel hydrophilic vs. conventional hydrophobic obturation systems: a bacterial leakage study. | comparative assessment of apical sealing ability of a novel smart-seal system, resilon, and conventional gutta-percha system using a bacterial leakage model. | 2017 | 25657530 |
| evaluation of antibacterial and antifungal activity of new calcium-based cement (biodentine) compared to mta and glass ionomer cement. | to evaluate the antibacterial and antifungal properties of calcium-based cement, biodentine (ca3sio2), compared to commercial glass ionomer cements (gics) and mineral trioxide aggregate (mta). | 2017 | 25657526 |
| behavior of listeria monocytogenes in a multi-species biofilm with enterococcus faecalis and enterococcus faecium and control through sanitation procedures. | the formation of mono-species biofilm (listeria monocytogenes) and multi-species biofilms (enterococcus faecium, enterococcus faecalis, and l. monocytogenes) was evaluated. in addition, the effectiveness of sanitation procedures for the control of the multi-species biofilm also was evaluated. the biofilms were grown on stainless steel coupons at various incubation temperatures (7, 25 and 39°c) and contact times (0, 1, 2, 4, 6 and 8 days). in all tests, at 7°c, the microbial counts were below 0.4 ... | 2015 | 25655573 |
| chemical composition and antibacterial activities of seven eucalyptus species essential oils leaves. | in this paper, we have studied the essential oils chemical composition of the leaves of seven eucalyptus species developed in tunisia. eucalyptus leaves were picked from trees growing in different arboretums in tunisia. choucha and mrifeg arboretums located in sedjnene, region of bizerte (choucha: e. maideni, e. astrengens et e. cinerea; mrifeg : e. leucoxylon), korbous arboretums located in the region of nabeul, north east tunisia with sub-humid bioclimate, (e. lehmani), souiniet-ain drahem arb ... | 2015 | 25654423 |
| enterococcus faecalis attenuates the differentiation of macrophages into osteoclasts. | enterococcus faecalis is closely associated with refractory apical periodontitis, manifesting periapical lesions and alveolar bone loss. macrophages playing an important role in the induction of inflammation can differentiate into bone-resorbing osteoclasts. in the present study, we investigated the effect of e. faecalis on the differentiation and function of macrophages as osteoclast precursors. | 2015 | 25649294 |
| decolorization and detoxification of sulfonated toxic diazo dye c.i. direct red 81 by enterococcus faecalis yz 66. | isolated enterococcus faecalis yz 66 strain shows ability to decolorize various industrial dyes among which, it showed complete decolorization and degradation of toxic, sulfonated recalcitrant diazo dye direct red 81 (50 mg/l) within 1.5 h of incubation under static anoxic condition. the optimum ph and temperature for decolorization was 7.0 and 40°c, respectively. significant induction in the activity of intracellular oxidoreductive enzymes suggested its involvement in the decolorization of dire ... | 2014 | 25649265 |
| extraction, characterization and antimicrobial activity of sulfated polysaccharides from fish skins. | sulfated polysaccharides were extracted from gray triggerfish (gtsp) and smooth hound (shsp) skins. their chemical and physical characteristics were determined using x-ray diffraction and infrared spectroscopic analysis. the antibacterial activities of gtsp and shsp against listeria monocytogenes (atcc 43251), staphylococcus aureus (atcc 25923), enterococcus faecalis (atcc 29212), escherichia coli (atcc 25922), salmonella enterica (atcc 43972) and enterobacter sp were evaluated by determining cl ... | 2015 | 25647621 |
| antibiofilm efficacy evaluation of a bioelectric dressing in mono- and multi-species biofilms. | chronic infections are often related to the formation of single or polymicrobial biofilms, which lead to hard-to-treat infections, difficult wound management, and recurrent infections. in this study, we evaluated in vitro antibiofilm properties of a silver and zinc bioelectric dressing (procellera). | 2015 | 25647504 |
| horizontal transfer of antibiotic resistance from enterococcus faecium of fermented meat origin to clinical isolates of e. faecium and enterococcus faecalis. | enterococcus species are part of the normal intestinal flora of a large number of mammals including humans and consequently, they can be used as indicators of faecal contamination in food and water for human consumption. their presence in large numbers in foods may indicate a lapse in sanitation and their ability to serve as a genetic reservoir of transferable antibiotic resistance is of concern. in the present study, enterococcus spp., isolated from commercially fermented meat and human clinica ... | 2015 | 25647243 |
| detection of vancomycin resistances in enterococci within 3 ½ hours. | vancomycin resistant enterococci (vre) constitute a challenging problem in health care institutions worldwide. novel methods to rapidly identify resistances are highly required to ensure an early start of tailored therapy and to prevent further spread of the bacteria. here, a spectroscopy-based rapid test is presented that reveals resistances of enterococci towards vancomycin within 3.5 hours. without any specific knowledge on the strain, vre can be recognized with high accuracy in two different ... | 2015 | 25645753 |
| β-lactam combinations with daptomycin provide synergy against vancomycin-resistant enterococcus faecalis and enterococcus faecium. | enterococcus faecalis (efc) and enterococcus faecium (efm) are frequently resistant to vancomycin and β-lactams (bls). in vitro data suggest synergy between several bls and glycopeptides or lipopeptides against resistant pathogens. our objective was to conduct combination mic and time-kill experiments to evaluate bl synergy with daptomycin against enterococci. | 2015 | 25645208 |
| antimicrobial activity of different disinfection methods against biofilms in root canals. | the aim of the present study was to evaluate the bactericidal effects of different disinfection methods against microbial biofilms grown in root canals. | 2016 | 25641829 |
| enterococcus faecalis with capsule polysaccharides type 2 and biofilm-forming capacity in indonesians requiring endodontic treatment. | the aim of the present study was to evaluate the genetic diversity of enterococcus faecalis (e. faecalis) strains isolated from saliva and infected root canal samples from indonesians requiring endodontic treatment. | 2015 | 25641820 |
| diagnostic and therapeutic utility of transrectal ultrasound in urological office prostatic abscess management: a short report from a single urologic center. | prostatic abscess (pa) is an infrequent condition in the modern antibiotic era. the everyday use of transrectal ultrasound (trus) during diagnostic work-up and the widespread recurrence to prostatic biopsies may lead to an increase of pa diagnosis. in this short report we analyze the patients characteristics and the management of seven recent cases of pa diagnosed in our institution. | 2014 | 25641468 |
| natural sesquiterpene lactones enhance oxacillin and gentamicin effectiveness against pathogenic bacteria without antibacterial effects on beneficial lactobacilli. | this is a report on the synergistic interactions (sis) between melampolide-type sesquiterpene lactones 1-8 from acanthospermum hispidum dc., and oxacillin or gentamicin, against four pathogenic strains of staphylococcus aureus and enterococcus faecalis; two of them were multi-resistant strains obtained from chronic infectious processes. our results showed that all associations of 1-8 with antibiotics (atbs) are more effective than pure atbs to control pathogenic strains of s. aureus and e. faeca ... | 2015 | 25641272 |
| isolation of alkaline-tolerant bacteria from primary infected root canals. | alkaline-tolerant bacteria in primary infected root canals could have enhanced survival capacity against antimicrobials commonly used in root canal treatment. the aims of this study were to isolate and characterize alkaline-tolerant bacteria before endodontic treatment (s1), after chemomechanical root canal preparation (s2), and after calcium hydroxide dressing (s3). | 2015 | 25638530 |
| optimizing supercritical carbon dioxide in the inactivation of bacteria in clinical solid waste by using response surface methodology. | clinical solid waste (csw) poses a challenge to health care facilities because of the presence of pathogenic microorganisms, leading to concerns in the effective sterilization of the csw for safe handling and elimination of infectious disease transmission. in the present study, supercritical carbon dioxide (sc-co2) was applied to inactivate gram-positive staphylococcus aureus, enterococcus faecalis, bacillus subtilis, and gram-negative escherichia coli in csw. the effects of sc-co2 sterilization ... | 2015 | 25636860 |
| antibacterial effect of butyryl alkannin from arnebia euchroma against vancomycin-resistant pathogens of enterococcus faecalis causing urinary tract infections. | this study was carried out to investigate the biomedicinal potential of a bioactive marker component, butyryl alkannin, isolated from n-hexane root extract of arnebia euchroma against various vancomycin-resistant enterococcus (vre) isolates of enterococcus faecalis causing urinary tract infections. as a result, butyryl alkannin showed significant antibacterial activity against multidrug-resistant e. faecalis pathogens of vre as minimum inhibitory concentration values which were found in the rang ... | 2015 | 25635653 |
| ef0176 and ef0177 from enterococcus faecalis v583 are substrate-binding lipoproteins involved in abc transporter mediated ribonucleoside uptake. | one of the abc transporter systems in enterococcus faecalis v583 is encoded by the ef0176-ef0180 gene cluster, which differs from orthologous operons in related bacteria in that it contains two genes putatively encoding substrate-binding proteins (sbps). these sbps, ef0176 and ef0177, have previously been identified on the surface of e. faecalis. by phenotypic studies of single and double knockout mutants, we show here that ef0176 and ef0177 are specific for ribonucleosides and, by inference, th ... | 2015 | 25635276 |
| direct detection and quantification of bacterial genes associated with inflammation in dna isolated from stool. | although predominantly associated with health benefits, the gut microbiota has also been shown to harbor genes that promote inflammation. in this work, we report a method for the direct detection and quantification of these pro-inflammatory bacterial genes by pcr and qpcr in dna extracted from human stool samples. pcr reactions were performed to detect (i) the pks island genes, (ii) tcpc, which is present in some strains of escherichia coli and (iii) gele presented in some strains of enterococcu ... | 2014 | 25635239 |
| in vitro activity of linezolid as assessed through the 2013 leader surveillance program. | the 2013 leader surveillance program monitored the in vitro activity of linezolid and comparator agents against gram-positive bacteria at 60 medical centers in the united states. a total of 7183 pathogens were contributed from 6 predetermined pathogen groups. the groups were staphylococcus aureus, coagulase-negative staphylococci, enterococci, streptococcus pneumoniae, β-hemolytic streptococci, and viridans group streptococci. the mic90 value for each of the 6 pathogen groups was 1 μg/ml. suscep ... | 2015 | 25633420 |
| antimicrobial activity of extracts and isoquinoline alkaloids of selected papaveraceae plants. | alkaloidal extracts of seven selected plants of the family papaveraceae were studied with respect to their activity against six strains of pathogenic bacteria and their alkaloidal fingerprint. twenty-four alkaloids were determined by gc/ms, and twenty of them identified from their mass spectra, retention times and retention indexes. in the antibacterial assay, three gram-positive (enterorococcus faecalis, staphylococcus aureus and s. hyicus), and three gram-negative (escherichia coli, proteus mi ... | 2014 | 25632464 |
| efficacy of contemporary and novel intracanal medicaments against enterococcus faecalis. | to compare the antibacterial activity of propolis (30% in methyl cellulose), curcumin (2.5mg/ml of methyl cellulose), 2% chlorhexidine gel (chx), 2% metronidazole gel (mz) and a mixture of 2% chx and 2% mz against enterococcus faecalis in vitro. calcium hydroxide served as the control. | 2014 | 25631726 |
| an in vivo comparison of antimicrobial efficacy of sodium hypochlorite and biopure mtad™ against enterococcus faecalis in primary teeth: a qpcr study. | biopure mtad™, a new root canal irrigant has shown promising results against the most common resistant microorganism, e. faecalis, in permanent teeth. however, there is lack of studies comparing its antimicrobial effectiveness with naocl in primary teeth. the purpose of this study was to compare the in vivo antimicrobial efficacy of naocl 2.5% and biopure mtad™ against e. faecalis in primary teeth. | 2014 | 25631723 |
| ex vivo evaluation of three instrumentation techniques on e. faecalis biofilm within oval shaped root canals. | the objective of the present study was to assess the effectiveness of reciprocating instrumentation in disinfecting oval-shaped root canals infected with enterococcus faecalis. forty-five human lower premolars were infected with a culture of e. faecalis (atcc 29212) for 28 days. five other teeth that were neither contaminated nor instrumented were used as controls. the 45 specimens were divided into three experimental groups (n = 15) based on the root canal preparation technique used: manual (k- ... | 2015 | 25627890 |
| update of the telavancin activity in vitro tested against a worldwide collection of gram-positive clinical isolates (2013), when applying the revised susceptibility testing method. | a revised broth microdilution susceptibility testing method for telavancin was approved by the food and drug administration (fda). telavancin activity was assessed against gram-positive pathogens collected worldwide (2013) using the revised method. a total of 12,346 isolates from 90 sites were included as part of the telavancin international surveillance program for the americas, europe, and asia-pacific. telavancin had mic50 and mic90 values of 0.03 and 0.06 μg/ml, respectively, against staphyl ... | 2015 | 25618421 |
| dietary enterococcus faecalis lab31 improves growth performance, reduces diarrhea, and increases fecal lactobacillus number of weaned piglets. | lactic acid bacteria (lab) have been shown to enhance performance of weaned piglets. however, few studies have reported the addition of lab enterococcus faecalis as alternatives to growth promoting antibiotics for weaned piglets. this study evaluated the effects of dietary e. faecalis lab31 on the growth performance, diarrhea incidence, blood parameters, fecal bacterial and lactobacillus communities in weaned piglets. a total of 360 piglets weaned at 26 ± 2 days of age were randomly allotted to ... | 2015 | 25617897 |
| synthesis of silver nanoparticles using solanum trilobatum fruits extract and its antibacterial, cytotoxic activity against human breast cancer cell line mcf 7. | in the present study, we have synthesized silver nanoparticles by a simple and eco-friendly method using unripe fruits of solanum trilobatum. the aqueous silver ions when exposed to unripe fruits extract were reduced and stabilized over long time resulting in biosynthesis of surface functionalized silver nanoparticles. the bio-reduced silver nanoparticles were characterized by uv-visible spectroscopy, fourier transform infrared spectroscopy (ftir), scanning electron microscopy (sem), transmissio ... | 2015 | 25613692 |
| gold nanoshell-decorated silicone surfaces for the near-infrared (nir) photothermal destruction of the pathogenic bacterium e. faecalis. | catheter-related infections (cris) are associated with the formation of pathogenic biofilms on the surfaces of silicone catheters, which are ubiquitous in medicine. these biofilms provide protection against antimicrobial agents and facilitate the development of bacterial resistance to antibiotics. the application of photothermal agents on catheter surfaces is an innovative approach to overcoming biofilm-generated cris. gold nanoshells (aunss) represent a promising photothermal tool, because they ... | 2015 | 25611157 |
| oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid. | oxidative stress can damage proteins, dna, and lipids, and is involved in the progression of many diseases. damage to infected cells caused by oxidative stress is related to increased levels of reactive oxygen species, including hydrogen peroxide. during oxidative stress, hydrogen peroxide levels are often increased and catalase level decreased inside cells. this can lead to the death of skin and other cells. hydrophobic low molecular weight compounds are useful in treating hemorrhagic condition ... | 2015 | 25609960 |
| antibiotic resistance prevalence in routine bloodstream isolates from children's hospitals varies substantially from adult surveillance data in europe. | surveillance of antimicrobial resistance (amr) is central for defining appropriate strategies to deal with changing amr levels. it is unclear whether childhood amr patterns differ from those detected in isolates from adult patients. | 2015 | 25607829 |
| urinary tract pathogens-sensitivity to trimethoprim/sulphamethoxazole. | abstract the sensitivity to sulphamethizole, sulphamethoxazole (sm), trimethoprim (tmp) and the combination sm/tmp of freshly isolated urinary tract pathogens from hospital patients was studied. out of 798 strains tested primarily, 47% were found to be fully sensitive to sulphamethizole (tablet method), and 81% fully sensitive to the sm/tmp combination (disk method). out of 423 strains with reduced sensitivity to sulphamethizole, 65% were fully sensitive to sm/tmp. out of 391 strains tested seco ... | 1970 | 25607580 |
| the disinfecting efficacy of root canals with laser photodynamic therapy. | infecting microorganisms of the root canals are difficult to eliminate during endodontic treatment. in this study the effect of root canal disinfection with photodynamic therapy (pdt) at different time intervals in comparison to 2.5% sodium hypochlorite (naocl) irrigation and passive ultrasonic irrigation (pui) in extracted teeth colonized with enterococcus faecalis and candida albicans was tested to assess which treatment reaches the best disinfection rate. | 2014 | 25606335 |
| fluoride varnish as root canal sealer: a scanning electron microscopy and bacterial penetration study. | this study was carried out to evaluate the bacterial leakage of root canal fillings when cavity varnish containing 5% fluoride (duraflur) was used as root canal sealer. | 2015 | 25598813 |
| antimicrobial activity of nanoparticle calcium hydroxide against enterococcus faecalis: an in vitro study. | enterococcus faecalis (e. faecalis) has the ability to invade the dentinal tubules and resist high ph levels. as a result, calcium hydroxide (ch) is not much effective on this bacterium. in theory, nanoparticle calcium hydroxide (nch) has smaller size and high surface area that enables it to penetrate into the deeper layers of dentin and be more effective on e. faecalis. this in vitro study was designed to compare the antimicrobial activity of nch and ch against e. faecalis. | 2015 | 25598808 |
| efficacy of 3d conforming nickel titanium rotary instruments in eliminating canal wall bacteria from oval-shaped root canals. | to evaluate the effectiveness of trushape® 3d conforming files, compared with twisted files, in reducing bacteria load from root canal walls, in the presence or absence of irrigant agitation. | 2015 | 25593001 |
| manual and rotary instrumentation ability to reduce enterococcus faecalis associated with photodynamic therapy in deciduous molars. | this aim of this study was to assess the ability of manual or rotary instrumentation associated with photodynamic therapy (pdt) to reduce enterococcus faecalis using three combinations of light/photosensitizers: toluidine blue o/laser, fuchsin/halogen light and fuchsin/led. twenty deciduous molars were selected and contaminated with enterococcus faecalis (mcfarland 0.5 scale). working length determination was performed by visual method. the teeth were randomly divided into two groups: g1 (n=10): ... | 2016 | 25590196 |
| microleakage of bacteria in different implant-abutment assemblies: an in vitro study. | the aim of the present in vitro study was to evaluate the leakage observed for 2 different microbial species at the level of the implant-abutment (i-a) interface, and the marginal fit and size of microgap at the i-a interface in 2 different implant connections. | 2015 | 25589158 |
| different vana elements in e. faecalis and in e. faecium suggest at least two origins of tn1546 among vre in a brazilian hospital. | in 2009 during surveillance in a brazilian hospital, many patients were confirmed to be colonized by vancomycin-resistant enterococci (vre) and a few infection cases occurred. among 14 isolates of enterococcus faecalis, most had the same pulsotype, virulence profile (agg(+)elra(+)gele(+)), and were sequence type (st)103, indicating dissemination of a clone. the 47 enterococcus faecium were separated into four pulsotypes, the predominant virulence profile being esp(+)acm(+). all of them harbored ... | 2015 | 25588068 |
| antibacterial effect of all-in-one self-etch adhesives on enterococcus faecalis. | background and aims. the aim of this study was to evaluate the antibacterial activity of one-step self-etch adhesives on enterococcus faecalis on days 1, 7 and 14 with the use of modified direct contact test. materials and methods. the modified directcontact test was used to evaluate the antibacterial effect of adper easy one, bond force, clearfil s3 bond, futurabond m, g-bond, ibond and optibond all-in-one adhesives on enterococcus faecalis after aging the samples in phosphate-buffered saline f ... | 2014 | 25587384 |
| chemical assessment and antimicrobial and antioxidant activities of endophytic fungi extracts isolated from costus spiralis (jacq.) roscoe (costaceae). | costus spiralis (costaceae) is a species native to the amazon region and is used in traditional medicine. the endophytic fungi used in this study were obtained from leaves of this plant. 13 strains were selected to obtain hydroethanolic extracts and were submitted to hydroalcoholic extraction and evaluated for antioxidant activity by dpph (2,2-difenil-1-picrilhidrazil) and frap (ferric reducing antioxidant power), and all of the fungi had positive results. the antimicrobial action of crude extra ... | 2014 | 25587339 |
| assessment of photodynamic therapy (pdt) in disinfection of deeper dentinal tubules in a root canal system: an in vitro study. | the success of endodontic treatment therapy depends on how well we eliminate pathogenic microflora from the root canal system as micro organism as the major cause of root canal infection. conventional root canal treatment can fail if microorganisms cannot be removed sufficiently by thorough cleaning, shaping of root canal. newer modalities such as photodynamic therapy are being tried now a days for disinfection of root canals. aim & objectives: the basic aim of this study was assessment of the a ... | 2014 | 25584321 |
| an in vitro evaluation of antimicrobial efficacy of 2% chlorhexidine gel, propolis and calcium hydroxide against enterococcus faecalis in human root dentin. | to evaluate in vitro the antimicrobial efficacy of 2% chlorhexidine gel, propolis and calcium hydroxide against enterococcus faecalis in human root dentin. | 2014 | 25584319 |
| antimicrobial activity and stability of electron beam irradiated dental irrigants. | the electron beam (e-beam) radiation is considered as an effective means of sterilization of healthcare products as well as to induce the structural changes in the pharmaceutical agents/drug molecules. in addition to structural changes of pharmaceutical it also induces the formation of low molecular weight compounds with altered microbiological, physicochemical and toxicological properties. among the several known medicaments, sodium hypochlorite (naocl) and chlorhexidine digluconate (chx) are u ... | 2014 | 25584220 |
| in vitro spectrum of pexiganan activity when tested against pathogens from diabetic foot infections and with selected resistance mechanisms. | pexiganan, a 22-amino-acid synthetic cationic peptide, is currently in phase 3 clinical trials as a topical antimicrobial agent for the treatment of mild infections associated with diabetic foot ulcers. bacterial isolates from the 2013 sentry antimicrobial surveillance program designated as pathogens from diabetic foot infections (dfi) and gram-negative and -positive pathogens from various infection types that harbored selected resistance mechanisms/phenotypes were tested against pexiganan in re ... | 2015 | 25583717 |
| characterisation of the efficacy of endodontic medications using a three-dimensional fluorescent tooth model: an ex vivo study. | the purpose of this study was to establish a three-dimensional fluorescent tooth model to investigate bacterial viability against intra-canal medicaments across the thickness and surface of root dentine. dental microbial biofilms (enterococcus faecalis and streptococcus mutans) were established on the external root surface and bacterial kill was monitored over time against intra-canal medicament (ca(oh)2 ) using fluorescent microscopy in conjunction with baclight syto9 and propidium iodide stain ... | 2015 | 25583457 |
| l-lactate production from biodiesel-derived crude glycerol by metabolically engineered enterococcus faecalis: cytotoxic evaluation of biodiesel waste and development of a glycerol-inducible gene expression system. | biodiesel waste is a by-product of the biodiesel production process that contains a large amount of crude glycerol. to reuse the crude glycerol, a novel bioconversion process using enterococcus faecalis was developed through physiological studies. the e. faecalis strain w11 could use biodiesel waste as a carbon source, although cell growth was significantly inhibited by the oil component in the biodiesel waste, which decreased the cellular nadh/nad(+) ratio and then induced oxidative stress to c ... | 2015 | 25576618 |
| an alkaline phosphatase reporter for use in clostridium difficile. | clostridium difficile is an anaerobic, gram-positive pathogen that causes severe gastrointestinal disease in humans and other mammals. c. difficile is notoriously difficult to work with and, until recently, few tools were available for genetic manipulation and molecular analyses. despite the recent advances in the field, there is no simple or cost-effective technique for measuring gene transcription in c. difficile other than direct transcriptional analyses (e.g., quantitative real-time pcr and ... | 2015 | 25576237 |
| the new schiff base 4-[(4-hydroxy-3-fluoro-5-methoxy-benzylidene)amino]-1,5-dimethyl-2-phenyl-1,2-dihydro-pyrazol-3-one: experimental, dft calculational studies and in vitro antimicrobial activity. | the synthesized schiff base, 4-[(4-hydroxy-3-fluoro-5-methoxy-benzylidene)amino]-1,5-dimethyl-2-phenyl-1,2-dihydro-pyrazol-3-one (i), has been characterized by (13)c nmr, (1)h nmr, 2d nmr ((1)h-(1)h cosy and (13)c apt), ft-ir, uv-vis and x-ray single-crystal techniques. molecular geometry of the compound i in the ground state, vibrational frequencies and chemical shift values have been calculated by using the density functional method (dft) with 6-311++g(d,p) basis set. the obtained results indi ... | 2015 | 25574656 |
| risk factors analysis for occurrence of asymptomatic bacteriuria after endourological procedures. | endourological procedures are performed according to the principles of aseptic techniques, jet still in certain number of patients urinary tract infections may occur. considering the risk of urinary tract infection, there is no unique opinion about the prophylactic use of antibiotics in endourological procedures. | 2014 | 25568546 |
| the catastrophic journey of a retained temporary epicardial pacemaker wire leading to enterococcus faecalis endocarditis and subsequent stroke. | a 52-year-old man underwent triple coronary artery bypass graft (cabg). three months later, he presented to the emergency room with dizziness and left-sided weakness. workup revealed right frontal parenchymal haemorrhage; also, two retained temporary epicardial pacemaker wires (tepw) from his previous cabg were observed, one of which had significantly migrated. one wire was found in the soft tissues below the heart; the other transversed the mediastinum from the expected location of the right at ... | 2015 | 25568268 |
| antibacterial and cytotoxic activities of terminalia stenostachya and terminalia spinosa. | plants that belong to the combretaceae family have long history of use in the traditional medicine systems of africa and asia for treatment of diseases and conditions associated with hiv/aids-opportunistic infections. the objective of this study was to investigate the biological activities of extracts of terminalia stenostachya engl. & diels and terminalia spinosa engl. (combretaceae), to verify the rationale for their use by traditional health practitioners in the treatment of hiv/aids patients ... | 2011 | 25566609 |
| susceptibility of glycopeptide-resistant enterococci to linezolid, quinupristin/dalfopristin, tigecycline and daptomycin in a tertiary greek hospital. | we investigated the antibiotic susceptibility of glycopeptide-resistant enterococci (gre). seventy consecutive gre were tested. sixty-two isolates were identified as enterococcus faecium (88.6%), and 8 (11.4%) as enterococcus faecalis. all strains were susceptible to linezolid and daptomycin, while 17.1% (12/70) and 11.4% (8/70) were resistant to quinupristin/dalfopristin (qd) and tigecycline, respectively. all e. faecalis isolates were resistant to qd, while 4 of 62 (6.5%) e. faecium isolates w ... | 2014 | 25566405 |
| drug resistance and molecular epidemiology of aerobic bacteria isolated from puerperal infections in bangladesh. | puerperal infection is a common complication during postnatal period in developing countries. bacterial species, drug resistance, and genetic characteristics were investigated for a total of 470 isolates from puerperal infections in bangladesh for a 2-year period (2010-2012). the most common species was escherichia coli (n=98), followed by enterococcus faecalis (n=54), staphylococcus haemolyticus (n=33), proteus mirabilis (n=32), staphylococcus aureus (n=27), klebsiella pneumoniae (n=22), and en ... | 2015 | 25555043 |
| antibacterial, antioxidant, anti-cholinesterase potential and flavonol glycosides of biscutella raphanifolia (brassicaceae). | different extracts of the aerial parts of biscutella raphanifolia (brassicaceae), which has not been the subject of any study, were screened for the phytochemical content, anti-microbial, antioxidant and anti-cholinesterase activities. we used four methods to identify the antioxidant activity namely, abts(•+), dpph• scavenging, cuprac and ferrous-ions chelating methods. since there is a relationship between antioxidants and cholinesterase enzyme inhibitors, we used two methods to determine the i ... | 2015 | 25553679 |
| characterization of ambrette seed oil and its mode of action in bacteria. | in the present study, chemical composition and the antibacterial mechanism of ambrette seed oil are investigated. chemical composition of the oil was analysed by gas chromatography-mass spectrometry (gc-ms). thirty-five compounds were identified and the major compounds were found to be farnesol acetate (51.45%) and ambrettolide (12.96%). the antibacterial activity was performed by well diffusion assay and the mechanisms were studied by measuring the alkaline phosphatase (alp), lactate dehydrogen ... | 2014 | 25551188 |
| nanobioarchitectures based on chlorophyll photopigment, artificial lipid bilayers and carbon nanotubes. | in the last decade, building biohybrid materials has gained considerable interest in the field of nanotechnology. this paper describes an original design for bionanoarchitectures with interesting properties and potential bioapplications. multilamellar lipid vesicles (obtained by hydration of a dipalmitoyl phosphatidylcholine thin film) with and without cholesterol were labelled with a natural photopigment (chlorophyll a), which functioned as a sensor to detect modifications in the artificial lip ... | 2014 | 25551059 |
| antimicrobial activity of prodigiosin isolated from serratia marcescens ufpeda 398. | prodigiosin is an alkaloid and natural red pigment produced by serratia marcescens. prodigiosin has antimicrobial, antimalarial and antitumor properties and induces apoptosis in t and b lymphocytes. these properties have piqued the interest of researchers in the fields of medicine, pharmaceutics and different industries. the aim of the present study was to evaluate the antimicrobial activity of prodigiosin against pathogenic micro-organisms. the red pigments produced by s. marcescens exhibited a ... | 2015 | 25549906 |
| [bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2010 and march 2011]. | bacteria isolated from surgical infections during the period from april 2010 to march 2011 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 631 strains including 25 strains of candida spp. were isolated from 170 (81.7%) of 208 patients with surgical infections. four hundred and twenty two strains were isolated from primary infections, and 184 strains were isolated from surgical site infection. from primary infections, anaerobic gram-nega ... | 2014 | 25549405 |
| detection of listeria monocytogenes with short peptide fragments from class iia bacteriocins as recognition elements. | we employed a direct peptide-bacteria binding assay to screen peptide fragments for high and specific binding to listeria monocytogenes. peptides were screened from a peptide array library synthesized on cellulose membrane. twenty four peptide fragments (each a 14-mer) were derived from three potent anti-listerial peptides, leucocin a, pediocin pa1, and curvacin a, that belong to class iia bacteriocins. fragment leu10 (geafsagvhrlang), derived from the c-terminal region of leucocin a, displayed ... | 2015 | 25548942 |
| population biology of intestinal enterococcus isolates from hospitalized and nonhospitalized individuals in different age groups. | the diversity of enterococcal populations from fecal samples from hospitalized (n = 133) and nonhospitalized individuals (n = 173) of different age groups (group i, ages 0 to 19 years; group ii, ages 20 to 59 years; group iii, ages ≥60 years) was analyzed. enterococci were recovered at similar rates from hospitalized and nonhospitalized persons (77.44% to 79.77%) of all age groups (75.0% to 82.61%). enterococcus faecalis and enterococcus faecium were predominant, although seven other enterococcu ... | 2015 | 25548052 |