Publications

overproduction of the rbo gene product from desulfovibrio species suppresses all deleterious effects of lack of superoxide dismutase in escherichia coli.	in an attempt to isolate the superoxide dismutase (sod) gene from the anaerobic sulfate-reducing bacterium desulfoarculus baarsii, a dna fragment was isolated which functionally complemented an escherichia coli mutant (soda sodb) deficient in cytoplasmic sods. this region carries two open reading frames with sequences which are very similar to that of the rbo-rub operon from desulfovibrio vulgaris. independent expression of the rbo and rub genes from ptac showed that expression of rbo was respon ...	1996	8955290
nmr studies of cooperativity in the tetrahaem cytochrome c3 from desulfovibrio vulgaris.	the thermodynamic properties of the desulfovibrio vulgaris (hildenborough) tetrahaem cytochrome c3 (dvc3) are rationalised by a model which involves both homotropic (e-/e-) and heterotropic (e-/h+) cooperativity. the paramagnetic shifts of a methyl group from each haem of the dvc3 have been determined in each stage of oxidation at several ph values by means of two-dimensional exchange nmr. the thermodynamic parameters are obtained by fitting the model to the nmr data and to redox titrations foll ...	1996	8944758
bidirectional transformation of aromatic aldehydes by desulfovibrio desulfuricans under nitrate-dissimilating conditions.	desulfovibrio desulfuricans atcc 27774 was screened for reactivity against aromatic compounds during lactate-dependent, nitrate-dissimilating growth. only aromatic aldehydes (benzaldehyde, 2-hydroxybenzaldehyde, 3-hydroxybenzaldehyde, 4-hydroxybenzaldehyde, vanillin, iso-vanillin and o-vanillin) were reactive and, with the exception of 2-hydroxybenzaldehyde, were stimulatory to lactate-dependent growth. aromatic aldehydes were transformed to their corresponding benzoate and benzyl alcohol deriva ...	1996	8936370
identity and interactions of rumen microbes associated with dietary sulfate-induced polioencephalomalacia in cattle.	to study their role in sulfate reduction, anaerobic bacteria were cultured from rumen fluid samples of cattle fed high-carbohydrate, short-fiber diets with and without added sulfate. the steers fed the diet with added sulfate developed polioencephalomalacia. microbiological methods included colony type profiles, molybdate sensitivity, presence of desulfoviridin, sulfate reduction rates of pure and mixed cultures, and incubation time effects on sulfate reduction. colony-type profiles indicated de ...	1995	8928959
degradation products of the cytochrome c3 mrna are similar in desulfovibrio vulgaris hildenborough and escherichia coli.	the transcription and mrna degradation pattern of a cloned desulfovibrio vulgaris (dv) hildenborough cytochrome c3-encoding gene (cyc) was analyzed in detail, both in escherichia coli and its native species. transcription in dv seems to be controlled by the same promoter elements as in e. coli; the transcription start point (tsp) of this dv gene has been mapped in both species and found to be identical. a major putative transcription terminator was mapped and it was found to be the same in both ...	1996	8921871
identification and characterization of [fe]-hydrogenases in the hydrogenosome of trichomonas vaginalis.		1996	8920017
distribution of sulfate-reducing bacteria in a stratified fjord (mariager fjord, denmark) as evaluated by most-probable-number counts and denaturing gradient gel electrophoresis of pcr-amplified ribosomal dna fragments.	the sulfate-reducing bacterial populations of a stratified marine water column, mariager fjord, denmark, were investigated by molecular and culture-dependent approaches in parallel. denaturing gradient gel electrophoresis (dgge) of pcr-amplified 16s rrna and dna encoding rrna (rdna) isolated from the water column indicated specific bacterial populations in different water column layers and revealed a highly differentiated pattern of rrna- and rdna-derived pcr amplificates, probably reflecting ac ...	1996	8919802
inhibition by iodoacetamide and acetylene of the h-d-exchange reaction catalyzed by thiocapsa roseopersicina hydrogenase.	the kinetics of h-d isotope exchange catalyzed by the thermostable hydrogenase from thiocapsa roseopersicina have been studied by analysis of the exchange between d2 and h2o. the ph dependence of the exchange reaction was examined between ph 2.5 and ph 11. over the whole ph range, hd was produced at a higher initial velocity than h2, with a marked optimum at ph 5.5; a second peak in the ph profile was observed at around ph 8.5. the rapid formation of h2 with respect to hd in the d2/h2o system is ...	1996	8917471
coinfection with intracellular desulfovibrio species and coccidia in ferrets with proliferative bowel disease.		1996	8905593
molecular identification of bacteria from a coculture by denaturing gradient gel electrophoresis of 16s ribosomal dna fragments as a tool for isolation in pure cultures.	molecular information about the bacterial composition of a coculture capable of sulfate reduction after exposure to oxic and microoxic conditions was used to identify and subsequently to isolate the components of the mixture in pure culture. pcr amplification of 16s ribosomal dna fragments from the coculture, analyzed by denaturing gradient gel electrophoresis, resulted in two distinct 16s ribosomal dna bands, indicating two different bacterial components. sequencing showed that the bands were d ...	1996	8900013
physiologic studies with the sulfate-reducing bacterium desulfovibrio desulfuricans: evaluation for use in a biofuel cell.	the growth kinetics of the sulfate-reducing bacteria desulfovibrio desulfuricans essex 6 was investigated under various conditions for potential use in a microbial fuel cell that recovers electrons generated from the reduction of sulfate to hydrogen sulfide. hydrogen sulfide was found to inhibit growth and decrease both the growth yields and the sulfate-specific reduction rate. hydrogen sulfide inhibition was direct, reversible, and not due to limitation by iron deficiency. a high initial lactat ...	1996	8882004
hydrogen sulphide: a bacterial toxin in ulcerative colitis?		1996	8881797
rnase e can inhibit the decay of some degradation intermediates: degradation of desulfovibrio vulgaris cytochrome c3 mrna in e coli.	in escherichia coli, ribonuclease e (rnase e) is a key endonuclease in mrna decay. we have analysed the role of e coli rnase e on the degradation of a heterologous cytochrome c3 (cyc) mrna from desulfovibrio vulgaris hildenborough. the decay of the cyc transcript in wild-type and mutant e coli cells was followed and the degradation intermediates analysed by northern blotting and s1 protection analysis. the half-life of total cyc mrna intermediates was increased in the rnase e mutant. a number of ...	1996	8874797
electron transfer in tetrahemic cytochromes c3: spectroelectrochemical evidence for a conformational change triggered by heme iv reduction.	electron transfer in tetrahemic cytochromes c3 from desulfovibrio vulgaris hildenborough (d.v.h.) and desulfovibrio desulfuricans norway (d.d.n.) strains has been investigated by thin layer spectroelectrochemistry with visible absorption, cd, and resonance raman (rr) monitoring. the observed splitting of the isosbestic point in the soret absorption band indicates that the electron transfer from the (feiii)4 state to the (feii)4 state proceeds via an intermediate species, which corresponds to 25 ...	1996	8873609
characterization of sulfate-reducing bacteria isolated from oil-field waters.	the occurrence and metabolic capacities of sulfate-reducing bacteria (srb) were studied in 23 water samples taken from producing wells at 14 different sites. oil fields in france, the north sea, and the gulf of guinea were selected and classified according to physicochemical parameters (salinity ranging from 0.3 to 120 g.l-1 and temperature between 29 and 85 degrees c). after the distribution of srb within oil fields was studied, several strains of srb were isolated and characterized metabolical ...	1996	8868233
activation and degradation of benzoate, 3-phenylpropionate and crotonate by syntrophus buswellii strain ga. evidence for electron-transport phosphorylation during crotonate respiration.	a strictly anaerobic, benzoate-degrading bacterium, syntrophus buswellii strain ga, was able to degrade benzoate or 3-phenylpropionate to acetate, co2 and h2, if the hydrogen partial pressure was sufficiently low. the hydrogen was removed in syntrophic coculture by methanospirillum hungatei or by desulfovibrio sp. through interspecies hydrogen transfer or in pure culture by the use of crotonate as reducible cosubstrate. alternatively, s. buswellii strain ga could grow in pure culture with croton ...	1996	8867639
new conformational properties induced by the replacement of tyr-64 in desulfovibrio vulgaris hildenborough ferricytochrome c553 using isotopic exchanges monitored by mass spectrometry.	in order to study the conformational stability induced by the replacement of tyr-64 in desulfovibrio vulgaris hildenborough (dvh) cytochrome c553, fast peptic digestion of deuterated protein followed by separation and measurement of related peptides using liquid chromatography coupled to electrospray ionization mass spectrometry was performed. we show that the h-bonding and/or solvent accessibility properties were modified by the single-site mutation. the mutant proteins can be classified into t ...	1996	8849688
transposon mutagenesis in desulfovibrio desulfuricans: development of a random mutagenesis tool from tn7.	the transposons tn5, tn7, tn9, and tn10 or their derivatives have been examined for transposition in the sulfate-reducing bacterium desulfovibrio desulfuricans g20. tn7 inserted with a frequency of 10(-4) to 10(-3) into a unique attachment site that shows strong homology with those sites identified in other gram-negative bacteria. inactivation of the tnsd gene in tn7, encoding the function directing insertion into the unique site, yielded a derivative that transposed essentially randomly with a ...	1996	8837431
degradative capacities and 16s rrna-targeted whole-cell hybridization of sulfate-reducing bacteria in an anaerobic enrichment culture utilizing alkylbenzenes from crude oil.	a mesophilic sulfate-reducing enrichment culture growing anaerobically on crude oil was used as a model system to study which nutritional types of sulfate-reducing bacteria may develop on original petroleum constituents in oil wells, tanks, and pipelines. chemical analysis of oil hydrocarbons during growth revealed depletion of toluene and o-xylene within 1 month and of m-xylene, o-ethyltoluene, m-ethyltoluene, m-propyltoluene, and m-isopropyltoluene within approximately 2 months. in anaerobic c ...	1996	8837415
the dcr gene family of desulfovibrio: implications from the sequence of dcrh and phylogenetic comparison with other mcp genes.	desulfovibrio vulgaris hildenborough contains a family of genes for methyl-accepting chemotaxis proteins (mcps). here we report the complete sequence of the gene for desulfovibrio chemoreceptor h (dcrh). the deduced amino acid sequence of dcrh protein, which has an enlarged n-terminal, ligand binding domain, indicates a structure similar to that of other mcps. comparison of the sequences for dcra, determined earlier, and dcrh indicated that similarity is essentially limited to the c-terminal exc ...	1996	8836438
evaluation of the electrostatic effect of the 5'-phosphate of the flavin mononucleotide cofactor on the oxidation--reduction potentials of the flavodoxin from desulfovibrio vulgaris (hildenborough).	two mutants of the desulfovibrio vulgaris flavodoxin, t12h and n14h, were generated which, for the first time, place a basic residue within the normally neutral 5'-phosphate binding loop of the flavin mononucleotide cofactor binding site found in all flavodoxins. these histidine residues were designed to form an ion pair with the dianionic 5'-phosphate, either altering its ionization state or offsetting its negative charge to allow evaluation of the magnitude of its electrostatic effect on the r ...	1996	8823179
characterization of microbial communities in anaerobic bioreactors using molecular probes.	the microbial community structure of twenty-one single-phase and one two-phase full-scale anaerobic sewage sludge digesters was evaluated using oligonucleotide probes complementary to conserved tracts of the 16s rrnas of phylogenetically defined groups of methanogens and sulfate-reducing bacteria. these probe results were interpreted in combination with results from traditional chemical analyses and metabolic activity assays. it was determined that methanogens in "healthy" mesophilic, single-pha ...	1995	8821785
comparison of low oxidoreduction potential cytochrome c553 from desulfovibrio vulgaris with the class i cytochrome c family.	the cytochrome c553 from desulfovibrio vulgaris (dvh c553) is of importance in the understanding of the relationship of structure and function of cytochrome c due to its lack of sequence homology with other cytochromes, and its abnormally low oxido-reduction potential. in evolutionary terms, this protein also represents an important reference point for the understanding of both bacterial and mitochondrial cytochromes c. using the recently determined nuclear magnetic resonance (nmr) structure of ...	1996	8820485
cytochrome c3 from desulfovibrio gigas: crystal structure at 1.8 a resolution and evidence for a specific calcium-binding site.	crystals of the tetraheme cytochrome c3 from sulfate-reducing bacteria desulfovibrio gigas (dg) (mw 13 kda, 111 residues, four heme groups) were obtained and x-ray diffraction data collected to 1.8 a resolution. the structure was solved by the method of molecular replacement and the resulting model refined to a conventional r-factor of 14.9%. the three-dimensional structure shows many similarities to other known crystal structures of tetraheme c3 cytochromes, but it also shows some remarkable di ...	1996	8819167
a structure-based catalytic mechanism for the xanthine oxidase family of molybdenum enzymes.	the crystal structure of the xanthine oxidase-related molybdenum-iron protein aldehyde oxido-reductase from the sulfate reducing anaerobic gram-negative bacterium desulfovibrio gigas (mop) was analyzed in its desulfo-, sulfo-, oxidized, reduced, and alcohol-bound forms at 1.8-a resolution. in the sulfo-form the molybdenum molybdopterin cytosine dinucleotide cofactor has a dithiolene-bound fac-[mo, = o, = s, ---(oh2)] substructure. bound inhibitory isopropanol in the inner compartment of the subs ...	1996	8799115
interactions of 77se and 13co with nickel in the active site of active f420-nonreducing hydrogenase from methanococcus voltae.	the selenium-containing f420-nonreducing hydrogenase from methanococcus voltae was prepared in the nia(i) middle dotco state. the effect of illumination on this light-sensitive species was studied. epr studies were carried out with enzyme containing natural selenium or with enzyme enriched in 77se. samples were prepared with either co or 13co. in the nia(i) middle dotco state, the nuclear spins of both 77se (i = 1/2) and 13c (i = 1/2) interacted with the nickel-based unpaired electron, suggestin ...	1996	8798608
redox properties of cytochrome c nitrite reductase from desulfovibrio desulfuricans atcc 27774.	the dissimilatory nitrite reductase from desulfovibrio desulfuricans atcc 27774 catalyzes the reduction of nitrite to ammonia. previous spectroscopic investigation revealed that it is a hexaheme cytochrome containing one high spin ferric heme and five low spin ferric hemes in the oxidized enzyme. the current study uses the high resolution of mössbauer spectroscopy to obtain redox properties of the six heme groups. correlating the mössbauer findings with the epr data reveals the pairwise spin-spi ...	1996	8798514
cloning, expression, and sequence analysis of the three genes encoding quinoline 2-oxidoreductase, a molybdenum-containing hydroxylase from pseudomonas putida 86.	the three genes coding for quinoline 2-oxidoreductase (qor) of pseudomonas putida 86 were cloned and sequenced. the qor genes are clustered in the transcriptional order medium (m) small (s), large (l) and code for three subunits of 288 (qorm), 168 (qors), and 788 (qorl) amino acids, respectively. formation of active quinoline 2-oxidoreductase and degradation of quinoline occurred in a recombinant p. putida kt2440 clone. the amino acid sequences of qor show significant homology to various prokary ...	1996	8798497
intraspecies variability of cellular fatty acids among soil and intestinal strains of desulfovibrio desulfuricans.	a comparison of cellular fatty acid profiles of desulfovibrio desulfuricans dsm 642 and 14 wild strains of this species, isolated from two completely different environments, soil and the human intestine, was carried out. all the d. desulfuricans strains grown on lactate and sulfate indicated the presence of considerable amounts of i-c15:0, i-c17:1 and c16:0. although differences in the quantities of individual fatty acids present in each strain were clear in the group of soil strains (similarity ...	1996	8795227
population dynamics of propionate-oxidizing bacteria under methanogenic and sulfidogenic conditions in anaerobic granular sludge.	laboratory-scale upflow anaerobic sludge-bed reactors were inoculated with industrial granular sludge and fed with either propionate or propionate and sulfate. the population dynamics of the propionate-oxidizing bacteria desulfobulbus sp. and the syntrophically growing strain syn7 were studied in reactors by dot blot and in situ hybridization with 16s rrna-based oligonucleotide probes.	1996	8787413
1h and 15n nmr resonance assignments and solution secondary structure of oxidized desulfovibrio desulfuricans flavodoxin.	sequence-specific 1h and 15n resonance assignments have been made for 137 of the 146 nonprolyl residues in oxidized desulfovibrio desulfuricans [essex 6] flavodoxin. assignments were obtained by a concerted analysis of the heteronuclear three-dimensional 1h-15n noesy-hmqc and tocsy-hmqc data sets, recorded on uniformly 15n-enriched protein at 300 k. numerous side-chain resonances have been partially or fully assigned. residues with overlapping 1hn chemical shifts were resolved by a three-dimensi ...	1996	8785498
probable new species of desulfovibrio isolated from a pyogenic liver abscess.	a fastidious, slowly growing, spiral gram-negative bacterium was isolated from the liver abscess of an 82-year-old man with a 3-week history of febrile illness. the organism was an obligate anaerobe that grew at 37 and 42 degrees c but not at 25 degrees c. its vibrioid or spiral morphology on gram staining, rapid progressive motility, electron micrograph features, and biochemical tests were all consistent with the organism belonging to the genus desulfovibrio. 16s rrna gene sequencing of this or ...	1996	8784584
desulfovibrio gabonensis sp. nov., a new moderately halophilic sulfate-reducing bacterium isolated from an oil pipeline.	two moderately halophilic sulfate-reducing bacteria were isolated from an african oil pipeline and designated strains sebr 3640 and sebr 2840t (t = type strain). both of these strains possess traits that define the genus desulfovibrio. the cells of both isolates were motile curved rods that had a single polar flagellum and contained desulfoviridin, and both isolates utilized lactate, pyruvate, malate, fumarate, succinate, and ethanol in the presence of sulfate. sulfite, thiosulfate, and elementa ...	1996	8782680
properties of xanthine dehydrogenase variants from rosy mutant strains of drosophila melanogaster and their relevance to the enzyme's structure and mechanism.	xanthine dehydrogenase, a molybdenum, iron-sulfur flavoenzyme encoded in the fruit fly drosophila melanogaster by the rosy gene, has been characterised both from the wild-type and mutant files. enzyme assays, using a variety of different oxidising and reducing substrates were supplemented by limited molecular characterisation. four rosy strains showed no detectable activity in any enzyme assay tried, whereas from four wild-type and three rosy mutant strains, those for the [e89k], [l127f] and [l1 ...	1996	8774727
investigation of oxidation state-dependent conformational changes in desulfovibrio vulgaris hildenborough cytochrome c553 by two-dimensional h-nmr spectra.	two-dimensional nuclear magnetic resonance spectroscopy (2d-nmr) was used to assign the proton resonances of ferricytochrome c553 from desulfovibrio vulgaris hildenborough. the spin systems of 76 out of 79 amino acids were identified by j-correlation spectroscopy (cosy and hohaha) in h20 and d20 and correlated by nuclear overhauser effect spectroscopy (noesy). the proton chemical shifts are compared in both oxidized and reduced states of the protein at 23 degrees c and ph 5.9. chemical shift var ...	1996	8766830
preliminary crystallographic analysis of the oxidized form of a two mono-nuclear iron centres protein from desulfovibrio desulfuricans atcc 27774.	crystals of the fully oxidized form of desulfoferrodoxin were obtained by vapor diffusion from a solution containing 20% peg 4000, 0.1 m hepes buffer, ph 7.5, and 0.2 m cacl2. trigonal and/or rectangular prisms could be obtained, depending on the temperature used for the crystal growth. trigonal prisms belong to the rhombohedral space group r32, with a = 112.5 a and c = 63.2 a; rectangular prisms belong to the monoclinic space group c2, with a = 77.7 a, b = 80.9 a, c = 53.9 a, and beta = 98.1 de ...	1996	8762151
genetic control of the resistance to phage c1 of escherichia coli k-12.	escherichia coli k-12 lytic phage c1 was earlier isolated in our laboratory. its adsorption is controlled by at least three bacterial genes: dcra, dcrb, and btub. our results provide evidence that the dcra gene located at 60 min on the e. coli genetic map is identical to the sdac gene. this gene product is an inner membrane protein recently identified as a putative specific serine transporter. the dcrb gene, located at 76.5 min, encodes a 20-kda processed periplasmic protein, as determined by ma ...	1996	8752353
[isolation and reassociation of acetogen and methanogen in a syntrophobic coculture degrading butyrate anaerobically].	anaerobic coculture bf2 which degraded butyrate into acetate and produced methane was isolated from granular methanogenic sludge. the coculture is associated syntrophically the syntrophomonas subsp. saponavida strain cf2 with methanobacterium formicicum strain mf2 and appeared to degraded c4 approximately c18 fatty acids including isobutyrate. the optimal temperature and ph for growth was 37 degrees c and 7.7 respectively. the strain cf2 was obtained in pure culture with crotonate as substrate a ...	1995	8745550
crystal structure of a dimeric octaheme cytochrome c3 (m(r) 26,000) from desulfovibrio desulfuricans norway.	the octaheme cytochrome c3 (m(r) 26,000; cc3) from desulfovibrio desulfuricans norway is a dimeric cytochrome made up of two identical subunits, each containing four heme groups. it is involved in the redox transfer chain of sulfate-reducing bacteria, which links the periplasmic oxidation of hydrogen to the cytoplasmic reduction of sulfate. the amino-acid sequence of cc3 shows similarities to that of the tetraheme cytochrome c3 (m(r) 13,000; c3) from the same bacteria. structural analysis of cc3 ...	1996	8740362
1h nmr investigation of the secondary structure, tertiary contacts and cluster environment of the four-iron ferredoxin from the hyperthermophilic archaeon thermococcus litoralis.	the solution molecular structure of the four-iron ferredoxin (fd) from the hyperthermophilic archaeon thermococcus litoralis (tl) has been investigated by 1h nmr spectroscopy. tocsy and noesy experiments in h2o, tailored to detect both weakly and strongly relaxed resonances, together with steady-state noes in both h2o and d2o, allowed the identification of 58 of the 59 residues, with one residue near the paramagnetic center undetected. it is shown that the contact shifted and strongly relaxed si ...	1996	8720830
nitrite reductase from desulfovibrio desulfuricans (atcc 27774)--a heterooligomer heme protein with sulfite reductase activity.	the membrane bound cytochrome c nitrite reductase from the sulfate reducer desulfovibrio desulfuricans (atcc 27774) was found to have a high specific activity in the reduction of sulfite, producing stoichiometric amounts of sulfide. the k(m) for sulfite in the mv+.:sulfite oxidoreductase assay is 0.75 mm, and the specific activity 2.06 mumolh2/min/mg. visible and epr spectroscopies studies indicate that the enzyme high-spin heme reacts with sulfite in the oxidised state, and that sulfide partial ...	1996	8713097
structural and functional characterization of cytochrome c3 from d. desulfuricans atcc 27774 by 1h-nmr.	cooperativity between redox and protonation centres is known to be crucial for the function of complex proteins, but it is often difficult to describe in terms of thermodynamic parameters. cytochrome c3 is a good model for these studies since, while retaining the overall complexity of larger systems, it is suitable for detailed crystallographic and spectroscopic studies. assignment of the haem substituent nmr resonances, together with nmr redox titrations of cytochrome c3 from d. desulfuricans a ...	1996	8706829
crystal structure of flavodoxin from desulfovibrio desulfuricans atcc 27774 in two oxidation states.	the crystal structures of the flavodoxin from desulfovibrio desulfuricans atcc 27774 have been determined and refined for both oxidized and semi-reduced forms to final crystallographic r-factors of 17.9% (0.8-0.205-nm resolution) and 19.4% (0.8-0.215-nm resolution) respectively. native flavodoxin crystals were grown from ammonium sulfate with cell constants a = b = 9.59 nm, c=3.37nm (oxidized crystals) and they belong to space group p3(2)21. semireduced crystals showed some changes in cell dimen ...	1996	8706707
dissimilatory sulfite reductase revisited. the desulfoviridin molecule does contain 20 iron ions, extensively demetallated sirohaem, and an s = 9/2 iron-sulfur cluster.	assimilatory sulfite reductase contains a sirohaem that is very weakly coupled to a [4fe-4s] cubane, i.e. five iron atoms in total. dissimilatory sulfite reductase is a complex system with 20 fe atoms/alpha 2 beta 2 gamma 2 hexamer. a recent revision of the purification procedure for the desulfovibrio vulgaris dissimilatory enzyme has afforded a preparation of only 10 fe atoms hexamer, this has led to the convulsion that the topology of prosthetic groups parallels that of the assimilatory system ...	1996	8706673
sulfonates: novel electron acceptors in anaerobic respiration.	the enrichment and isolation in pure culture of a bacterium, identified as a strain of desulfovibrio, able to release and reduce the sulfur of isethionate (2-hydroxyethanesulfonate) and other sulfonates to support anaerobic respiratory growth, is described. the sulfonate moiety was the source of sulfur that served as the terminal electron acceptor, while the carbon skeleton of isethionate functioned as an accessory electron donor for the reduction of sulfite. cysteate (alanine-3-sulfonate) and s ...	1996	8703197
nmr investigation of the solution conformation of oxidized flavodoxin from desulfovibrio vulgaris. determination of the tertiary structure and detection of protein-bound water molecules.	desulfovibrio vulgaris flavodoxin has been investigated with a combination of homo- and hetero-nuclear two-dimensional and three-dimensional nmr spectroscopy. the analysis of noe, hydrogen exchange and j-coupling data led to a set of 1349 noe, 63 hydrogen bond and 109 backbone phi-angle restraints which were used to determine the solution structure of the oxidized flavodoxin applying the distance geometry program diana combined with restrained energy minimization methods. flavodoxin in solution ...	1996	8681954
reaction of apoflavodoxin from desulfovibrio vulgaris with 5,5'-dithio-bis-(2-nitrobenzoic acid).		1996	8674695
the redox potentials of flavodoxin from desulfovibrio vulgaris and ferredoxin-nadp+ reductase from spinacia oleracea and their complexes.		1996	8674686
the effects of ph on the absorption spectrum of the hydroquinone of flavodoxin from desulfovibrio vulgaris.		1996	8674675
regulation of the redox potentials of flavodoxins: modification of the flavin binding.		1996	8674606
nmr studies of flavoproteins.		1996	8674598
tungsten in biological systems.	tungsten (atomic number 74) and the chemically analogous and very similar metal molybdenum (atomic number 42) are minor yet equally abundant elements on this planet. the essential role of molybdenum in biology has been known for decades and molybdoenzymes are ubiquitous. yet, it is only recently that a biological role for tungsten has been established in prokaryotes, although not as yet in eukaryotes. the best characterized organisms with regard to their metabolism of tungsten are certain specie ...	1996	8672295
total synthesis of a miniferredoxin.	a miniferredoxin has been designed based on the desulfovibrio gigas ferredoxin ii structure and has been successfully synthesized. the 31 amino acid apoprotein was synthesized via standard fmoc solid phase peptide synthesis and in vitro cluster insertion carried out. the uv-visible spectrum of the miniferredoxin (peak at 300 nm and a shoulder at 405 nm) shows the same features as that of the d. gigas ferredoxin. cyclic voltammetry indicated a quasireversible electrode process with a midpoint pot ...	1996	8670287
purification and characterization of the hydrogenase from thiobacillus ferrooxidans.	hydrogenase of thiobacillus ferrooxidans atcc 19859 was purified from cells grown lithoautotrophically with 80% hydrogen, 8.6% carbon dioxide, and 11.4% air. hydrogenase was located in the 140,000 x g supernatant in cell-free extracts. the enzyme was purified 7.3-fold after chromatography on procion red and q-sepharose with a yield of 19%, resulting in a 85% pure preparation with a specific activity of 6.0 u (mg protein)-1. with native page, a mol. mass of 100 and 200 kda was determined. with sd ...	1996	8661919
expression in escherichia coli and characterization of a reconstituted recombinant 7fe ferredoxin from desulfovibrio africanus.	desulfovibrio africanus ferredoxin iii is a monomeric protein (molecular mass of 6585 da) that contains one [3fe-4s]1+/0 and one [4fe-4s]2+/1+ cluster when isolated aerobically. the amino acid sequence consists of 61 amino acids, including seven cysteine residues that are all involved in co-ordination to the clusters. in order to isolate larger quantities of d. africanus ferredoxin iii, we have overexpressed it in escherichia coli by constructing a synthetic gene based on the amino acid sequence ...	1996	8660311
regulation of the redox order of four hemes by ph in cytochrome c3 from d. vulgaris miyazaki f.	the assignment of 1h-nmr signals of the heme methyl and propionate groups of cytochrome c3 of d. vulgaris miyazaki f was performed. the heme assignment was revised for hemes 2 and 3 (sequential heme numbering). namely, heme 4 is mainly reduced at first with hemes 1, 2 and 3 following it in this order. the p2h titration of heme methyl signals in four macroscopic oxidation states was performed in the p2h range of 5.2 to 9.0. while the heme methyl resonances in the fully oxidized state showed just ...	1996	8652627
primary structure of desulfoferrodoxin from desulfovibrio desulfuricans atcc 27774, a new class of non-heme iron proteins.	the primary structure of desulfoferrodoxin from desulfovibrio desulfuricans atcc 27774, a redox protein with two mononuclear iron sites, was determined by automatic edman degradation and mass spectrometry of the composing peptides. it contains 125 amino acid residues of which five are cysteines. the first four, cys-9, cys-12, cys-28 and cys-29, are responsible for the binding of center i which has a distorted tetrahedral sulfur coordination similar to that found in desulforedoxin from d. gigas. ...	1996	8647238
an nmr-derived model for the solution structure of oxidized thermotoga maritima 1[fe4-s4] ferredoxin.	the solution structure of the 60-residue 1[fe4-s4] ferredoxin from the hyperthermophilic bacterium thermotoga maritima was determined based on 683 distance and 35 dihedral angle restraints that were obtained from nmr data. in addition, data known from crystallographic studies of ferredoxins was used for modeling of the iron-sulfur cluster and its environment. the protein shows a globular fold very similar to the fold of the related 1[fe4-s4] ferredoxins from desulfovibrio gigas and desulfovibrio ...	1996	8647119
similarities in the architecture of the active sites of ni-hydrogenases and fe-hydrogenases detected by means of infrared spectroscopy.	three groups that absorb in the 2100-1800-cm-1 infrared spectral region have recently been detected in ni-hydrogenase from chromatium vinosum [bagley, k.a., duin, e.c., roseboom, w., albracht, s. p.j. & woodruff, w.h. (1995) biochemistry 34, 5527-5535]. to assess the significance and generality of this observation, we have carried out an infrared-spectroscopic study of eight hydrogenases of three different types (nickel, iron and metal-free) and of 11 other iron-sulfur and/or nickel proteins. in ...	1996	8647106
the structure of desulfovibrio vulgaris rubrerythrin reveals a unique combination of rubredoxin-like fes4 and ferritin-like diiron domains.	we have determined the structure of rubrerythrin, a non-haem iron protein from the anaerobic sulphate-reducing bacterium, desulfovibrio vulgaris (hildenborough), by x-ray crystallography. the structure reveals a tetramer of two-domain subunits. each subunit contains a four-helix bundle surrounding a diiron-oxo site and a c-terminal rubredoxin-like fes4 domain. the diiron-oxo site contains a larger number of carboxylate ligands and a higher degree of solvent exposure than do those in other diiron ...	1996	8646540
molecular characterization and transcriptional analysis of the putative hydrogenase gene of clostridium acetobutylicum atcc 824.	a 2.8-kbp dna region of clostridium acetobutylicum atcc 824 containing the putative hydrogenase gene (hyda) was cloned and sequenced. the 1,745-bp hyda encodes a 64,415-da protein and presents strong identity with the [fe] hydrogenase genes of desulfovibrio and clostridium species. the level of the putative hyda mrna was high in cells from an acidogenic or an alcohologenic phosphate-limited continuous culture, while it was comparatively very low in cells from a solventogenic phosphate-limited co ...	1996	8626337
characterization of representative enzymes from a sulfate reducing bacterium implicated in the corrosion of steel.	this communication reports the isolation, purification and characterization of key enzymes involved in dissimilatory sulfate reduction of a sulfate reducing bacterium classified as desulfovibrio desulfuricans subspecies desulfuricans new jersey (ncimb 8313) (ddd nj). the chosen strain, originally recovered from a corroding cast iron heat exchanger, was grown in large scale batch cultures. physico-chemical and spectroscopic studies of the purified enzymes were carried out. these analyses revealed ...	1996	8619870
heteronuclear relayed e.cosy applied to the determination of accurate 3j(hn,c') and 3j(h beta,c') coupling constants in desulfovibrio vulgaris flavodoxin.	a simple constant-time 3d heteronuclear nmr pulse sequence has been developed to quantitatively determine the heteronuclear three-bond couplings 3j(hn,c') and 3j(h beta,c') in uniformly 13c-enriched proteins. the protocols for measuring accurate coupling constants are based on 1h,13c-heteronuclear relayed e.cosy [schmidt, j.m., ernst, r.r., aimoto, s. and kainosho, m. (1995) j. biomol. nmr, 6, 95-105] in combination with numerical least-squares spectrum evaluation. accurate coupling constants ar ...	1996	8616270
kinetics and thermodynamics of the binding of riboflavin, riboflavin 5'-phosphate and riboflavin 3',5'-bisphosphate by apoflavodoxins.	the reactions of excess apoflavodoxin from desulfovibrio vulgaris, anabaena variabilis and azotobacter vinelandii with riboflavin 5'-phosphate (fmn), riboflavin 3',5'-bisphosphate and riboflavin are pseudo-first-order. the rates increase with decreasing ph in the range ph 5-8, and, in general, they increase with increasing ionic strength to approach a maximum at an ionic strength greater than 0.4 m. the rate of fmn binding in phosphate at high ph increases to a maximum at an ionic strength of ab ...	1996	8611166
sulfate-reducing bacteria in the periodontal pocket.	this report is the first to describe the occurrence of sulfate-reducing bacteria in the human mouth. samples of subgingival dental plaque were examined for the presence of sulfate-reducing bacteria. using enrichment cultures, sulfate-reducing bacteria were detected in 25 (58%) of 43 individuals, and in 39 (48%) of the 82 samples. pure isolates of sulfate-reducing bacteria, obtained from a limited number of enrichment cultures, belonged to the genera desulfobacter and desulfovibrio. these genera ...	1995	8596671
biochemical studies of the c-type cytochromes of the sulfate reducer desulfovibrio africanus. characterization of two tetraheme cytochromes c3 with different specificity.	three c-type cytochromes were isolated and characterized from the sulfate reducer desulfovibrio africanus. a basic tetraheme cytochrome c3 of molecular mass 16 kda was previously described and we have extended its characterization. two other c3-type cytochromes, not previously observed, have also been characterized. these include an acidic tetraheme cytochrome c3 of molecular mass 15 kda and an octaheme dimeric cytochrome c3 with a native size of 35 kda. this is the first report of the presence ...	1996	8573595
study of the new stability properties induced by amino acid replacement of tyrosine 64 in cytochrome c553 from desulfovibrio vulgaris hildenborough using electrospray ionization mass spectrometry.	hydrogen/deuterium exchange as well as charge state distribution monitored by electrospray ionization mass spectrometry were demonstrated to be a powerful and effective new tool for probing conformational properties of proteins in solution. in this paper, the influence of single amino acid replacements on the global conformation of cytochrome c553 from desulfovibrio vulgaris hildenborough using isotopic exchange monitored by electrospray ionization mass spectrometry is reported. based on their r ...	1996	8573183
purification and characterization of the formate dehydrogenase from desulfovibrio vulgaris hildenborough.	formate dehydrogenase from desulfovibrio vulgaris hildenborough, a sulfate-reducing bacterium, has been isolated and characterized. the enzyme is composed of three subunits. a high molecular mass subunit (83,500 da) is proposed to contain a molybdenum cofactor, a 27,000 da subunit is found to be similar to the fe-s subunit of the formate dehydrogenase from escherichia coli and a low molecular mass subunit (14,000 da) holds a c-type heme. the presence of heme c in formate dehydrogenase is reporte ...	1995	8566699
biodegradation of nitroaromatic compounds.	nitroaromatic compounds are released into the biosphere almost exclusively from anthropogenic sources. some compounds are produced by incomplete combustion of fossil fuels; others are used as synthetic intermediates, dyes, pesticides, and explosives. recent research revealed a number of microbial systems capable of transforming or biodegrading nitroaromatic compounds. anaerobic bacteria can reduce the nitro group via nitroso and hydroxylamino intermediates to the corresponding amines. isolates o ...	1995	8561470
molecular properties of the dissimilatory sulfite reductase from desulfovibrio desulfuricans (essex) and comparison with the enzyme from desulfovibrio vulgaris (hildenborough).	the dissimilatory sulfite reductase desulfoviridin was purified from the membrane (msir) and the soluble fraction (ssir) of the sulfate-reducing bacterium desulfovibrio desulfuricans (essex). molecular and spectroscopic properties were determined and compared with the properties of the soluble desulfoviridin from desulfovibrio vulgaris (hildenborough). the enzymes were isolated as alpha 2 beta 2 gamma n (n = 1-3) multimers with a relative molecular mass of 200 +/- 10 (gel filtration). both msir ...	1995	8521853
establishing isostructural metal substitution in metalloproteins using 1h nmr, circular dichroism, and fourier transform infrared spectroscopy.	far-uv cd, 1h-nmr, and fourier transform infrared (ftir) spectroscopy are three of the most commonly used methods for the determination of protein secondary structure composition. these methods are compared and evaluated as a means of establishing isostructural metal substitution in metalloproteins, using the crystallographically defined rubredoxin from desulfovibrio gigas and its well-characterized cadmium derivative as a model system. it is concluded that analysis of the ftir spectrum of the p ...	1995	8520483
modeling the structure of pyrococcus furiosus rubredoxin by homology to other x-ray structures.	the three-dimensional structure of rubredoxin from the hyperthermophilic archaebacterium, pyrococcus furiosus, has been modeled from the x-ray crystal structures of three homologous proteins from clostridium pasteurianum, desulfovibrio gigas, and desulfovibrio vulgaris. all three homology models are similar. when comparing the positions of all heavy atoms and essential hydrogen atoms to the recently solved crystal structure (day, m. w., et al., 1992, protein sci. 1, 1494-1507) of the same protei ...	1993	8518735
rubredoxin oxidase, a new flavo-hemo-protein, is the site of oxygen reduction to water by the "strict anaerobe" desulfovibrio gigas.	a rubredoxin-oxygen oxidoreductase, a homodimer with a molecular weight of 43 kda per monomer, was found to be a component of an electron transfer chain that couples the reduction of oxygen to water with nadh oxidation. this fad-containing protein appears to contain a new type of heme group. the electron transfer chain is not inhibited by cyanide and azide. in contrast, co decreases nadh oxidation rate and also induces release of the prosthetic groups from the native terminal reductase.	1993	8503894
analysis of the periplasmic [nife] hydrogenase transcription unit from desulfovibrio fructosovorans.	two genes, hyna and hynb, encode the two subunits of the periplasmic [nife] hydrogenase in desulfovibrio fructosovorans. sequencing downstream from hynb revealed a third open reading frame (hync) that has the potential for encoding a polypeptide showing 21% identity with the hyad, hoxm, and hupd proteins, belonging to putative operons encoding escherichia coli hydrogenase 1, alcaligenes eutrophus h16 membrane-bound hydrogenase, and rhizobium leguminosarum uptake hydrogenase, respectively. northe ...	1993	8501043
nitroaromatic compounds serve as nitrogen source for desulfovibrio sp. (b strain).	a sulfate-reducing bacterium, desulfovibrio sp. (b strain), isolated from a continuous anaerobic digester, used various nitroaromatic compounds such as 2,4-dinitrophenol, 2,4-dinitrotoluene, and 2,6-dinitrotoluene as sole nitrogen sources for growth and also used these compounds as electron acceptors in the absence of sulfate in the culture medium. more than 60% of the nitroaromatics were transformed within 6 days of incubation. the organism also used aniline as sole nitrogen source, but not as ...	1993	8500012
purification and characterization of an oxygen-labile, nad-dependent alcohol dehydrogenase from desulfovibrio gigas.	a nad-dependent, oxygen-labile alcohol dehydrogenase was purified from desulfovibrio gigas. it was decameric, with subunits of m(r) 43,000. the best substrates were ethanol (km, 0.15 mm) and 1-propanol (km, 0.28 mm). n-terminal amino acid sequence analysis showed that the enzyme belongs to the same family of alcohol dehydrogenases as zymomonas mobilis adh2 and bacillus methanolicus mdh.	1993	8491707
resonance raman studies of iron-only hydrogenases.	the nature of the iron-sulfur clusters in oxidized and reduced forms of fe-only hydrogenases from desulfovibrio vulgaris, thermotoga maritima, and clostridium pasteurianum has been investigated by resonance raman spectroscopy. the results indicate the presence of ferredoxin-like [4fe-4s]2+,+ and [2fe-2s]2+,+ clusters in both t. maritima hydrogenase and c. pasteurianum hydrogenase i, but only [4fe-4s]2+,+ clusters in d. vulgaris hydrogenase. this necessitates a reevaluation of the iron-sulfur clu ...	1993	8490025
homonuclear and heteronuclear nmr studies of oxidized desulfovibrio vulgaris flavodoxin. sequential assignments and identification of secondary structure elements.	recombinant desulfovibrio vulgaris flavodoxin (molecular mass 16.3 kda) was produced in escherichia coli. the oxidized protein has been investigated with a combination of homonuclear and heteronuclear two-dimensional and heteronuclear three-dimensional nmr spectroscopy. sequence-specific assignment of all backbone and most of the side chain 1h and 15n resonances has been obtained. the secondary structure has been inferred from the pattern of sequential, medium-, and long-range noes, together wit ...	1993	8477691
1h and 15n resonance assignments and solution secondary structure of oxidized desulfovibrio vulgaris flavodoxin determined by heteronuclear three-dimensional nmr spectroscopy.	sequence-specific 1h and 15n resonance assignments have been made for all 145 non-prolyl residues and for the flavin cofactor in oxidized desulfovibrio vulgaris flavodoxin. assignments were obtained by recording and analyzing 1h-15n heteronuclear three-dimensional nmr experiments on uniformly 15n-enriched protein, ph 6.5, at 300 k. many of the side-chain resonances have also been assigned. observed medium-and long-range noes, in combination with 3jnh alpha coupling constants and 1hn exchange dat ...	1993	8477184
a novel parameterization scheme for energy equations and its use to calculate the structure of protein molecules.	a novel scheme for the parameterization of a type of "potential energy" function for protein molecules is introduced. the function is parameterized based on the known conformations of previously determined protein structures and their sequence similarity to a molecule whose conformation is to be calculated. once parameterized, minima of the potential energy function can be located using a version of simulated annealing which has been previously shown to locate global and near-global minima with ...	1993	8441753
complete oxidation of benzoate and 4-hydroxybenzoate by a new sulfate-reducing bacterium resembling desulfoarculus.	a new sulfate-reducer "strain sax" was isolated from an anaerobic marine sediment [saxild, denmark]. the isolate was a gram-negative, motile and non-spore-forming rod which sometimes appeared as a curved rod. strain sax differed from all described desulfovibrio-, desulfobotulus- and desulfoarculus-species by the ability to degrade aromatic compounds such as benzoate, 4-hydroxybenzoate and phenol completely to co2. electron donors used included lactate, pyruvate, malate, fumarate, crotonate and b ...	1993	8439232
cobalamin-mediated mercury methylation by desulfovibrio desulfuricans ls.	the prominence of sulfate reducers in mercury biomethylation prompted the examination of the methyl carrier and mercury methylation activity of desulfovibrio desulfuricans ls. there was a low degree of mercury tolerance and a high degree of methylation during fermentative growth; the opposite was true during sulfate reduction. during 2 days of fermentative growth, up to 37% of hgcl2 was methylated at 0.1 micrograms/ml, but only 1.5% was methylated at 10.0 micrograms/ml. less than 1% of the added ...	1993	8439155
expression of the gamma-subunit gene of desulfoviridin-type dissimilatory sulfite reductase and of the alpha- and beta-subunit genes is not coordinately regulated.	it has been shown [pierik, a. j., duyvis, m. g., van helvoort, j. m. l. m., wolbert, r. b. g. & hagen, w. r. (1992) eur. j. biochem. 205, 111-115] that desulfoviridin, the dissimilatory sulfite reductase of sulfate-reducing bacteria of the genus desulfovibrio, contains a third, gamma, subunit (11 kda), in addition to the well-established alpha (50 kda) and beta (40 kda) subunits, and an alpha 2 beta 2 gamma 2 subunit structure has been proposed. cloning and sequencing of the dsvc gene indicated ...	1993	8436111
flavin dynamics in reduced flavodoxins. a time-resolved polarized fluorescence study.	the time-resolved fluorescence and fluorescence anisotropy characteristics of reduced flavin mononucleotide in solution as well as bound in flavodoxins isolated from the bacteria desulfovibrio gigas, desulfovibrio vulgaris, clostridium beijerinckii mp and megasphaera elsdenii have been examined. all fluorescence and fluorescence anisotropy decays were analyzed by two different methods: (a) least-squares fitting with a sum of exponentials and (b) the maximum entropy method to yield distributed li ...	1993	8425547
carboxy-terminal processing of the large subunit of [nife] hydrogenases.	two electrophoretic forms of the large subunit of the soluble periplasmic [nife] hydrogenase from desulfovibrio gigas have been detected by western analysis. the faster moving form co-migrates with the large subunit from purified, active enzyme. amino acid sequence and composition of the c-terminal tryptic peptide of the large subunit from purified hydrogenase revealed that it is 15 amino acids shorter than that predicted by the nucleotide sequence. processing of the nascent large subunit occurs ...	1993	8405419
environmental scanning electron microscope (esem) evaluation of crystal and plaque formation associated with biocorrosion.	the biofilm attributed to desulfovibrio vulgaris growing in the presence of ferrous metals was examined with an environmental scanning electron microscope. this novel microscope produced images of iron sulfide colloids and other iron containing structures that had not been reported previously. a plaque composed of iron sulfide enveloped the surface of the corroding metal while crystals containing magnesium, iron, sulfur, and phosphorus were present in the culture where corrosion was in progress. ...	1993	8400436
characterization of d. desulfuricans (atcc 27774) [nife] hydrogenase epr and redox properties of the native and the dihydrogen reacted states.	redox intermediates of d. desulfuricans atcc 27774 [nife] hydrogenase were generated under dihydrogen. detailed redox titrations, coupled to epr measurements, give access to the mid-point redox potentials of the iron-sulfur centers and of the nickel-b signal that represents the ready form of the enzyme. the interaction between the dihydrogen molecule and the nickel centre was probed by the observation of an isotopic effect on the epr signals detected in turnover conditions, by comparison of the ...	1993	8399280
the dissimilatory sulfite reductase from desulfosarcina variabilis is a desulforubidin containing uncoupled metalated sirohemes and s = 9/2 iron-sulfur clusters.	the active site of escherichia coli nadph-sulfite reductase has previously been modeled as a siroheme with its iron bridged to a nearby iron-sulfur cubane, resulting in antiferromagnetic exchange coupling between all iron atoms. the model has been suggested to hold also for other sulfite reductases and nitrite reductases. we have recently challenged the generality of the model with the finding that the epr of fe/s in dissimilatory sulfite reductase (desulfoviridin) from desulfovibrio vulgaris in ...	1993	8399175
structural studies on desulfovibrio gigas cytochrome c3 by two-dimensional 1h-nuclear-magnetic-resonance spectroscopy.	several aromatic amino acid residues and haem resonances in the fully reduced form of desulfovibrio gigas cytochrome c3 are assigned, using two-dimensional 1h n.m.r., on the basis of the interactions between the protons of the aromatic amino acids and the haem protons as well as the intrahaem distances known from the x-ray structure [kissinger (1989) ph.d. thesis, washington state university]. the interhaem interactions observed in the n.m.r. spectra are in full agreement with the d. gigas x-ray ...	1993	8397514
characterization of the structure and redox behaviour of cytochrome c3 from desulfovibrio baculatus by 1h-nuclear-magnetic-resonance spectroscopy.	complete assignment of the aromatic and haem proton resonances in the cytochromes c3 isolated from desulfovibrio baculatus strains (norway 4, dsm 1741) and (dsm 1743) was achieved using one- and two-dimensional 1h n.m.r. nuclear overhauser enhancements observed between haem and aromatic resonances and between resonances due to different haems, together with the ring-current contributions to the chemical shifts of haem resonances, support the argument that the haem core architecture is conserved ...	1993	8397513
intramolecular electron transfer in ferredoxin ii from desulfovibrio desulfuricans norway.	in order to elucidate the role of the two (4fe-4s) clusters in ferredoxins and to determine whether an electron-transfer mechanism may occur between the clusters, the in vitro reduction of cytochrome c3 and cytochrome c553 by desulfovibrio desulfuricans norway ferredoxin ii was studied using spectrophotometric techniques. ferredoxin ii, covalently cross-linked with either cytochrome c3 or c553, is an obligate intermediate in cytochrome reduction by pyruvate dehydrogenase. both titration of the c ...	1993	8396440
protein conformational changes in tetraheme cytochromes detected by ftir spectroelectrochemistry: desulfovibrio desulfuricans norway 4 and desulfovibrio gigas cytochromes c3.	the conformational change coupled to the redox processes of two tetraheme cytochromes c3 from bacteria of the genus desulfovibrio have been studied by uv-vis and ftir difference spectroscopy combined with protein electrochemistry. two pairs of equivalent hemes were found in desulfovibrio desulfuricans norway 4 cytochrome c3 by uv-vis spectroelectrochemical redox titration in an optically transparent thin-layer electrochemical cell. in contrast to this, desulfovibrio gigas cytochrome c3 showed a ...	1993	8396427
spectroscopic characterization of 57fe-reconstituted rubrerythrin, a non-heme iron protein with structural analogies to ribonucleotide reductase.	rubrerythrin, a contraction of rubredoxin and hemerythrin, is the trivial name given to a non-heme iron protein isolated from desulfovibrio vulgaris (hildenborough). this protein, whose physiological function is unknown, was first characterized by j. legall et al. [(1988) biochemistry 28, 1636] as being a homodimer of subunit m(r) = 21,900 with four fe per homodimer distributed as two rubredoxin-type fes4 centers and one hemerythrin-type diiron cluster. subsequent analysis of the amino acid sequ ...	1993	8395205
electron transport components of the parasitic protozoon giardia lamblia.	the energy metabolism of the intestinal parasite, giardia lamblia, involves the iron-sulphur protein, pyruvate:ferredoxin oxidoreductase. cell fractionation studies showed that this enzyme is associated with the membranes. nadh and nadph dehydrogenases were found in both the membrane and cytosolic fractions. epr spectroscopic studies showed the presence of iron-sulphur clusters in the membrane fraction and in the cytosolic fraction, non-sedimentable at 6 x 10(6) g.min. an acidic, soluble protein ...	1993	8391475
rapid comparison of the cytochrome c3 gene from nine strains of desulfovibrio vulgaris using polymerase chain reaction amplification.	polymerase chain reaction amplification was used to compare different regions of the cytochrome c3 gene from nine strains of desulfovibrio vulgaris, to examine homology within the species. six 30-base polymerase chain reaction primers and three probes were synthesized on the basis of the published nucleic acid sequence of the cytochrome c3 gene from d. vulgaris, ncimb 8303. amplifications were performed on genomic dna isolated from ncimb 8303 as well as eight other strains. six strains, ncimb 83 ...	1993	8388770
isolation and characterization of flavoredoxin, a new flavoprotein that permits in vitro reconstitution of an electron transfer chain from molecular hydrogen to sulfite reduction in the bacterium desulfovibrio gigas.	a new fmn-containing flavoprotein isolated from desulfovibrio gigas provided maximum coupling efficiency for the reduction of bisulfite from molecular h2. this protein, which is distinct from flavodoxin and for which the name flavoredoxin is proposed, is required for reconstitution of an electron transfer chain between hydrogenase and bisulfite reductase. a ca(2+)-binding protein functions as a modulator in the presence of ca2+ in the process. the finding of a membrane-bound cytochrome c with a ...	1993	8387752
amino-acid sequence of cytochrome c-553 from desulfovibrio desulfuricans norway.	the amino-acid sequence of the cytochrome c-553 from desulfovibrio desulfuricans norway has been determined and compared with that of two different cytochromes c-553 from d. vulgaris already described and with that cytochrome c-551 from pseudomonas. this low-molecular-weight monohemic cytochrome comprises 80 amino acids and has the typical characteristics of small cytochromes such as mitochondrial cytochromes. secondary-structure predictions are deduced from sequence data and are compared with x ...	1993	8383535
electrochemical studies of the hexaheme nitrite reductase from desulfovibrio desulfuricans atcc 27774.	the electron-transfer kinetics between three different mediators and the hexahemic enzyme nitrite reductase isolated from desulfovibrio desulfuricans (atcc 27774) were investigated by cyclic voltammetry and by chronoamperometry. the mediators, methyl viologen, desulfovibrio vulgaris (hildenborough) cytochrome c3 and d. desulfuricans (atcc 27774) cytochrome c3 differ in structure, redox potential and charge. the reduced form of each mediator exchanged electrons with nitrite reductase. second-orde ...	1993	8383043
nigerythrin and rubrerythrin from desulfovibrio vulgaris each contain two mononuclear iron centers and two dinuclear iron clusters.	the trivial name 'rubr-erythrin' is a contraction of two other trivial names: rubredoxin (ruber, red) and hemerythrin. it names a protein of undetermined biological function which putatively carries rubredoxin-like mononuclear iron and hemerythrin-like dinuclear iron. the name 'nigerythrin' (niger, black) is an analogy of rubrerythrin. it identifies a second protein of undetermined function which has prosthetic groups similar to rubrerythrin. rubrerythrin was initially described [legall, j., pri ...	1993	8383040
purification and characterization of an nadh-rubredoxin oxidoreductase involved in the utilization of oxygen by desulfovibrio gigas.	an nadh--rubredoxin oxidoreductase previously isolated from desulfovibrio gigas [legall, j. (1968) ann. inst. pasteur 114, 109-115] has now been fully purified and further characterized. it contains two subunits of 27 kda and 32 kda. with two mid-point redox potentials of -295 mv and -325 mv, this fmn- and fad-containing protein can induce the specific reduction of d. gigas rubredoxin. in contrast, rubredoxins from the other desulfovibrio species or desulforedoxin from d. gigas show very low rea ...	1993	8375383