Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| prevalence and antimicrobial resistance of foodborne pathogens isolated from food products in china. | this study was undertaken to investigate the prevalence of salmonella, listeria monocytogenes, staphylococcus aureus, vibrio parahaemolyticus, and escherichia coli o157:h7 in chinese food products. the prevalence of these pathogens was 3.46%, 5.79%, 7%, 0.24%, and 0%, respectively. raw meats were mainly contaminated with salmonella (39/365, 10.7%), l. monocytogenes (26/365, 7.1%), and s. aureus (40/365, 11%), while cooked food products were mainly contaminated with l. monocytogenes (45/384, 11.7 ... | 2007 | 17883311 |
| vopa inhibits atp binding by acetylating the catalytic loop of mapk kinases. | the bacterial pathogen vibrio parahemeolyticus manipulates host signaling pathways during infections by injecting type iii effectors. one of these effectors, vibrio outer protein a (vopa), inhibits mapk signaling via a novel mechanism, distinct from those described for other bacterial toxins, that disrupts this signaling pathway. vopa is an acetyltransferase that potently inhibits mapk signaling pathways not only by preventing the activation of mapk kinases (mkks) but also by inhibiting the acti ... | 2007 | 17881352 |
| [distribution, serotype and virulent genotype of vibrio parahaemolyticus in commercial seafood in zhoushan]. | 2007 | 17877188 | |
| [acute vibrio parahaemolyticus gastroenteritis in dakar]. | the aim of this study was to describe the epidemiological, clinical and bacteriological features of gastroenteritis due to vibrio parahaemolyticus diagnosed during the 2004 and 2005 cholera outbreak in senegal. | 2007 | 17855034 |
| molecular characterization of goose-type lysozyme homologue of large yellow croaker and its involvement in immune response induced by trivalent bacterial vaccine as an acute-phase protein. | lysozyme acts as an innate immunity molecule against the invasion of bacterial pathogens. here, the cdna of a goose-type lysozyme (g-lysozyme) was cloned from large yellow croaker (pseudosciana crocea) by expressed sequence tags (est) and race-pcr techniques. the full-length cdna of large yellow croaker g-lysozyme (lycgl) is 716 nucleotides (nt) encoding a protein of 193 amino acids (aa), with a theoretical molecular weight of 21.3kda. the deduced lycgl possessed the typical structural features ... | 2007 | 17850883 |
| rumphellolides a-f, six new caryophyllane-related derivatives from the formosan gorgonian coral rumphella antipathies. | six new caryophyllane-related natural products, including two carboxylated sesquiterpenoids, rumphellolides a (1) and b (2), and four norsesquiterpene alcohols, rumphellolides c-f (3-6), were isolated from the formosan gorgonian coral rumphella antipathies. the structures of the above new natural products were established on the basis of extensive spectral data analysis. rumphellolides a (1), d (4), e (5), and f (6) showed weak antibacterial activity. | 2007 | 17827751 |
| antimicrobial susceptibilities of vibrio parahaemolyticus and vibrio vulnificus isolates from louisiana gulf and retail raw oysters. | the antimicrobial susceptibilities of 168 vibrio parahaemolyticus and 151 vibrio vulnificus isolates recovered from 82 louisiana gulf and retail oysters in 2005 and 2006 were determined. overall, the two vibrios remained susceptible to the majority of antimicrobials tested; reduced susceptibility was detected only in v. parahaemolyticus for ampicillin (81%; mic > or = 16 microg/ml). additionally, v. parahaemolyticus displayed significantly higher mics for cefotaxime, ciprofloxacin, and tetracycl ... | 2007 | 17827331 |
| identification of a novel endochitinase from a marine bacterium vibrio proteolyticus strain no. 442. | chitin binding proteins prepared from vibrio proteolyticus were purified and the n-terminal amino-acid sequence of a protein from a 110-kda band on sds-page was found to be 85-90% identical to the 22nd-41st residues of the n-termini of chitinase a precursor proteins from other vibrios. we cloned the corresponding gene, which encodes a putative protein of 850 amino acids containing a 26-residue signal sequence. the chitinase precursor from v. proteolyticus was 78-80% identical to those from vibri ... | 2007 | 17690022 |
| a novel multiplex pcr assay for rapid and simultaneous detection of five pathogenic bacteria: escherichia coli o157:h7, salmonella, staphylococcus aureus, listeria monocytogenes, and vibrio parahaemolyticus. | rapid and sensitive detection techniques for foodborne pathogens are important to the food industry. however, traditional detection methods rely on bacterial culture in combination with biochemical tests, a process that typically takes 4 to 7 days to complete. thus, this study was conducted to address the issue of time lag inherent in traditional methods by developing a novel pcr assay for each of five foodborne pathogenic bacteria. this new system consists of a simultaneous screening method usi ... | 2007 | 17685339 |
| inhibitory effect of melastoma candidum d. don acetone extract on foodborne pathogenic bacteria survival in food products. | melastoma candidum d. don, a taiwanese folk medicinal plant, has high levels of antibacterial and bactericidal activity. our aim was to determine whether and to what extent an acetone extract of this plant inhibits the growth of foodborne pathogenic bacteria. m. candidum acetone extract had marked inhibitory effect on test bacteria introduced into sliced pork, which was then stored at 4 degrees c. at the end of storage (day 12), the bacterial concentrations dropped by 1.59 to 2.91 log cfu/g comp ... | 2007 | 17685331 |
| nitrogen fixation by vibrio parahaemolyticus and its implications for a new ecological niche. | a vibrio parahaemolyticus strain isolated from the rhizosphere of the ecosystem dominant estuarine grass, spartina alterniflora, was characterized and shown to carry nifh, the gene encoding the nitrogenase iron protein, and to fix n(2). nitrogen fixation may contribute substantially to the adaptability, niche breadth, and ecological significance of v. parahaemolyticus. | 2007 | 17675440 |
| [analysis of major phenotypes and hemolysin-related genotypes of vibrio parahaemolyticus isolates from clinical and seafood samples]. | vibrio parahaemolyticus is a gram-negative, halophilic bacterium that inhabits the marine and estuarine environments. it is an important human pathogen causing gastroenteritis when raw or partially-cooked seafoods are consumed. its pathogenicity is believed to be related to hemolysins such as thermostable direct hemolysin (tdh), tdh-related hemolysin (trh) and thermolabile hemolysin (tlh). pcr method was used to examine three different hemolysin genes in isolates from clinical and seafood sample ... | 2007 | 17672315 |
| analysis of bacterial foodborne disease outbreaks in china between 1994 and 2005. | to gain an understanding of the outbreaks of bacterial foodborne diseases and the subsequent health impact, we reviewed 2447 papers from journals published in china that reported 1082 bacterial foodborne disease cases occurring between 1994 and 2005. among the 1082 outbreaks of bacterial foodborne disease for which the etiology was determined, vibrio parahaemolyticus caused the most outbreaks, followed by salmonella, and clostridium botulinum led to the most deaths. most of the outbreaks occurre ... | 2007 | 17666075 |
| intragenomic heterogeneity and intergenomic recombination among vibrio parahaemolyticus 16s rrna genes. | vibrio parahaemolyticus is a marine bacterium bearing 11 copies of ribosomal operons. in some strains, such as rimd2210633, the genome includes identical copies of 16s rrna genes (rrs). however, it is known that other strains of the species, such as strains atcc 17802 and rimd2210856, show conspicuous intragenomic rrs heterogeneity. the extent and diversity of the rrs heterogeneity in v. parahaemolyticus were studied in further detail by characterization of the rrs copies in environmental isolat ... | 2007 | 17660428 |
| [pathogenic vibrios in oysters (crassostrea rhizophorae) served at restaurants in rio de janeiro: a public health warning]. | forty oyster samples (crassostrea rhizophorae) served raw in 15 restaurants in the city of rio de janeiro were evaluated in order to investigate the presence of vibrio spp. the oyster samples were analyzed and subjected to enrichment in alkaline peptone water with the addition of 1 and 3% nacl and incubated at 37 degrees c for 24 hours. following this, the cultures were seeded onto thiosulfate citrate bile sucrose agar (tcbs) and the suspected colonies were subjected to biochemical characterizat ... | 2007 | 17653465 |
| identification and characterization of vopt, a novel adp-ribosyltransferase effector protein secreted via the vibrio parahaemolyticus type iii secretion system 2. | vibrio parahaemolyticus strain rimd2210633 has two sets of genes encoding two separate type iii secretion systems (t3sss), called t3ss1 and t3ss2. t3ss2 has a role in enterotoxicity and is present only in kanagawa phenomenon-positive strains, which are pathogenic to humans. accordingly, t3ss2 is considered to be closely related to v. parahaemolyticus human pathogenicity. despite this, the biological actions of t3ss2 and the identity of the effector protein(s) secreted by this system have not bee ... | 2007 | 17645751 |
| development of a multiplex real-time pcr assay with an internal amplification control for the detection of total and pathogenic vibrio parahaemolyticus bacteria in oysters. | vibrio parahaemolyticus is an estuarine bacterium that is the leading cause of shellfish-associated cases of bacterial gastroenteritis in the united states. our laboratory developed a real-time multiplex pcr assay for the simultaneous detection of the thermolabile hemolysin (tlh), thermostable direct hemolysin (tdh), and thermostable-related hemolysin (trh) genes of v. parahaemolyticus. the tlh gene is a species-specific marker, while the tdh and trh genes are pathogenicity markers. an internal ... | 2007 | 17644647 |
| seaweeds as a reservoir for diverse vibrio parahaemolyticus populations in japan. | gastroenteritis caused by vibrio parahaemolyticus has recently been associated with foods prepared with seaweeds, but little is known about the bacterium's abundance and diversity among seaweeds in coastal environment. therefore, we determined its phenotypic and genotypic diversity in relation to its seasonal abundance in seawater and seaweed samples from three areas of kii channel, japan during june 2003 to may 2004. isolates were obtained by selective enrichment of samples and detection of v. ... | 2007 | 17629976 |
| research and characterization of pathogenic vibrios from bathing water along the conero riviera (central italy). | the occurrence and pathogenicity of vibrios in bathing water were investigated along the conero riviera (adriatic sea, central italy). vibrio spp. enumeration was performed on thiosulfate-citrate-bile-salts-sucrose-agar by the membrane filter method, and identification was done through a biochemical protocol. all isolates were tested for the presence of cytotoxicity, protease, lipase, elastase, gelatinase, urease, haemolytic activity, ctx, tdh and trh genes by conventional methods. in all, 200 v ... | 2007 | 17619048 |
| rapid and specific identification of 5 human pathogenic vibrio species by multiplex polymerase chain reaction targeted to dnaj gene. | a multiplex polymerase chain reaction (pcr) method, specifically designed for application in routine diagnostic laboratories, was developed for identifying 5 human pathogen vibrio species: vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio mimicus, and vibrio alginolyticus. this assay directed toward the dnaj gene was tested on a total of 355 strains representing 13 vibrio species and 17 non-vibrio species. specific pcr fragments were produced in isolates belonging to the 5 targ ... | 2007 | 17614235 |
| a targeting approach for delivery of polymer microparticle-antibody conjugate against vibrio parahaemolyticus-induced cytotoxicity to human intestinal epithelial cells. | a major traditional of antibacterial drugs is antibiotic which promotes more rapid release of the toxins from bacteria cells in human body, which causes severe infection. the thermostable direct hemolysin (tdh) has been proposed as a major virulence factor of vibrio parahaemolyticus (vp). this study covers the preparation of polymer microparticle-antibody conjugate for the development of a drug targeting approach for antibacterial drug delivery. the chemical binding of antibodies (ab) to latex b ... | 2007 | 17613661 |
| pathogenic bacteria associated with oysters (crassostrea brasiliana) and estuarine water along the south coast of brazil. | oysters and estuarine water samples were collected monthly, from june 1998 to march 1999, in the cananéia estuary, on the south coast of são paulo, brazil, and analyzed for bacterial hazards with and without depuration in filtered estuarine water. aeromonas spp., plesiomonas shigelloides, vibrio cholerae o1, vibrio parahaemolyticus, and vibrio vulnificus were counted in oyster samples using the most probable number (mpn) and their presence verified in the surrounding estuarine water samples. the ... | 2007 | 17613090 |
| isolation, sequencing and characterization of cluster genes involved in the biosynthesis and utilization of the siderophore of marine fish pathogen vibrio alginolyticus. | in fish pathogen vibrio alginolyticus mvp01, the isolated 11-gene cluster consisted of two divergently transcribed, fe(3+) and ferric uptake regulator (fur) regulated operons, pvsabcde and psua-pvuabcde, sharing high similarity with that related to siderophore biosynthesis and transportation locus in v. parahaemolyticus. siderophore biosynthesis or utilization was blocked when pvsa and pvsd of the pvsabcde operon or pvua, pvub and pvue of the psua-pvuabcde operon was single-gene in-frame mutated ... | 2007 | 17593352 |
| comparison of four antibiotics with indigenous marine bacillus spp. in controlling pathogenic bacteria from shrimp and artemia. | use of antibiotics for the control of bacterial diseases in shrimp culture has caused several adverse impacts to the industry. this has resulted in the search for alternative environment friendly approaches to overcome bacterial infections. this study was conducted to investigate the use of beneficial bacteria as an alternative to antibiotics. ten pathogenic bacterial species isolated from shrimp, penaeus monodon, and artemia cysts were tested for susceptibility to indigenous marine bacillus sub ... | 2007 | 17584435 |
| vibrios of the spotted rose snapper lutjanus guttatus steindachner, 1869 from northwestern mexico. | to characterize and identify vibrios present in wild and cultured juvenile snappers (lutjanus guttatus) in northwestern mexico. | 2007 | 17578416 |
| catecholamine-induced stimulation of growth in vibrio species. | to evaluate the effect of norepinephrine (ne) and related compounds on the growth of bacteria, we have examined the effect of the neuroendocrine hormone ne and related compounds on the growth of vibrio parahaemolyticus and other human-pathogenic vibrio species (vibrio cholerae, vibrio vulnificus, and vibrio mimicus). | 2007 | 17576228 |
| distribution, genetic richness and phage sensitivity of vibrio spp. from coastal british columbia. | this study examined the distribution, susceptibility to viral infection and genetic diversity of vibrio spp. in the coastal waters and sediments of british columbia during summer (july and august). abundances of presumptive vibrio spp. ranged from 1.5 to 346 ml(-1) within the water column (1-291 m); whereas, abundances at the water-sediment interface were much higher (up to approximately 3 x 10(4)vibrio spp. cc(-1)), and decreased with sediment depth (down to 30 cm). the genetic diversity of vib ... | 2007 | 17564612 |
| vibrio parahaemolyticus o3:k6 epidemic diarrhea, chile, 2005. | 2007 | 17561569 | |
| real-time reverse transcription-pcr for transcriptional expression analysis of virulence and housekeeping genes in viable but nonculturable vibrio parahaemolyticus after recovery of culturability. | a real-time reverse transcription-pcr method was developed to determine whether the recovery of culturability of viable but nonculturable (vbnc) vibrio parahaemolyticus induced the expression of virulence genes coding for the thermostable direct hemolysin and for type iii secretion system 2 (ttss2). the culturability of clinical strain vp5 of v. parahaemolyticus in artificial seawater at 4 degrees c was monitored, and the vbnc state was obtained. one day after entry into the vbnc state, temperat ... | 2007 | 17557845 |
| effect of the addition of four potential probiotic strains on the survival of pacific white shrimp (litopenaeus vannamei) following immersion challenge with vibrio parahaemolyticus. | four bacterial strains isolated from the gastrointestinal tract of adult shrimp litopenaeus vannamei, vibrio alginolyticus utm 102, bacillus subtilis utm 126, roseobacter gallaeciensis slv03, and pseudomonas aestumarina slv22, were evaluated for potential use as probiotics for shrimp. in vitro studies demonstrated antagonism against the shrimp-pathogenic bacterium, vibrio parahaemolyticus ps-017. feeding shrimp with diets containing the potential probiotics showed the best feed conversion ratio ... | 2007 | 17544437 |
| detection of tdh and trh genes in vibrio parahaemolyticus isolated from corbicula moltkiana prime in west sumatera, indonesia. | the occurrence of vibrio parahaemolyticus in raw corbicula moltkiana prime from lake singkarak and pasar raya padang market and in cooked samples in west sumatera, indonesia, was studied. thirteen raw and seven cooked bivalve samples were positive using chromagar vibrio medium. all 47 v parahaemolyticus isolates were positive for toxr gene but negative for trh. however, 36% (17/47) of v parahaemolyticus strains were positive for tdh gene. antibiotic profiling showed that 76% and 38% of isolates ... | 2007 | 17539286 |
| vibrio parahaemolyticus strains isolated during investigation of the summer 2006 seafood related diarrhea outbreaks in two regions of chile. | nine hundred cases of seafood related diarrhea were reported in the region of puerto montt, chile during the austral summer of 2006. this is the continuation of the large outbreaks associated with the consumption of seafood containing the vibrio parahaemolyticus serovar o3:k6 pandemic clonal group that arose last decade in chile. the initial outbreaks occurred during the summer of 1998 in antofagasta (23 degrees 39's 70 degrees 24'w). subsequently, outbreaks there were rare, but since 2004 outbr ... | 2007 | 17521760 |
| new role of c-terminal 30 amino acids on the insoluble chitin hydrolysis in actively engineered chitinase from vibrio parahaemolyticus. | a chitinase (vpchia) and its c-terminal truncated g589 mutant (vpchiag589) of vibrio parahaemolyticus were cloned by polymerase chain reaction (pcr) techniques. to study the role of the c-terminal 30 amino acids of vpchia in the enzymatic hydrolysis of chitin, both the recombinant vpchia and vpchiag589 encoded in 1,881 and 1,791 bp dna fragments, respectively, were expressed in escherichia coli using the pet-20b(+) expression system. the his-tag affinity purified vpchia and vpchiag589 enzymes ha ... | 2007 | 17508209 |
| an efficient system for markerless gene replacement applicable in a wide variety of enterobacterial species. | we describe an improved allelic-exchange method for generating unmarked mutations and chromosomal dna alterations in enterobacterial species. initially developed for use in salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. we have extended its use into related bacterial species that are more recalcitrant to genetic manipulations, including enterohemorrhagic and enteropathogenic escherichia coli and vibrio parahaemolyticus. data from over 50 experiments ... | 2007 | 17496950 |
| [characteristics of vibrio parahaemolyticus isolated from mussels (perna perna) commercialized at niterói, rio de janeiro]. | the marine ecosystem is the natural habitat of bacteria like vibrio parahaemolyticus, an important pathogen that cause human gastroenteritis associated with seafood consumption. in the present investigation, the presence of v. parahaemolyticus in 86 in natural and precooked mussel samples was evaluated. vibrio parahaemolyticus was isolated from 11.6% of the in natural and precooked mussels. all strains tested were urease-positive and 28.5% were kanagawa-positive, which suggests that they have pa ... | 2007 | 17486255 |
| microbiological study of cooked crustaceans and molluscan shellfish from uk production and retail establishments. | a study of cooked crustaceans and molluscan shellfish from retail and production premises was undertaken in the uk during 2003 to determine the microbiological safety of these products. according to criteria in european commission recommendation 2003/10/ec, 96% of 682 batches were of satisfactory/acceptable microbiological status while 4% of batches were unsatisfactory due to the presence of high levels of escherichia coli (3.3%; 10(2) to 10(6) cfu g(-1)), vibrio parahaemolyticus (0.4%; 10(2) to ... | 2007 | 17479385 |
| production of a recombinant chitin oligosaccharide deacetylase from vibrio parahaemolyticus in the culture medium of escherichia coli cells. | an open reading frame (orf) encoding chitin oligosaccharide deacetylase (pa-cod) gene and its signal sequence was cloned from the vibrio parahaemolyticus kn1699 genome and its sequence was analyzed. the orf encoded a 427 amino acid protein, including the 22 amino acid signal sequence. the deduced amino acid sequence was highly similar to several bacterial chitin oligosaccharide deacetylases in carbohydrate esterase family 4. an expression plasmid containing the gene was constructed and inserted ... | 2007 | 17479220 |
| characteristics of virulent vibrio parahaemolyticus isolated from oregon and washington. | thirty-four virulent strains of vibrio parahaemolyticus containing tdh and/or trh genes isolated from oregon and washington coastal water were analyzed for o-group antigens and urease activity, and by pulsed-field gel electrophoresis. six o serotypes (o1, o3, o4, o5, o10, and o11) were identified among the isolates, with the o5 group (19 isolates) being the most prevalent, followed by the 01 group (9 isolates). nearly all (33 of 34) isolates were capable of producing urease, which reaffirmed the ... | 2007 | 17477276 |
| an evaluation of the use of remotely sensed parameters for prediction of incidence and risk associated with vibrio parahaemolyticus in gulf coast oysters (crassostrea virginica). | the u.s. food and drug administration recently published a vibrio parahaemolyticus risk assessment for consumption of raw oysters that predicts v. parahaemolyticus densities at harvest based on water temperature. we retrospectively compared archived remotely sensed measurements (sea surface temperature, chlorophyll, and turbidity) with previously published data from an environmental study of v. parahaemolyticus in alabama oysters to assess the utility of the former data for predicting v. parahae ... | 2007 | 17477256 |
| cloning, expression and immunogenicty analysis of five outer membrane proteins of vibrio parahaemolyticus zj2003. | genes of five outer membrane proteins of vibrio parahaemolyticus zj2003, including ompw, ompv, ompk, ompu and tolc, were cloned and expressed as n-terminal his(6)-tagged proteins in escherichia coli. the recombinant fusion proteins were purified with nickel chelate affinity chromatography. to analyze the immunogenicity of these proteins, large yellow croaker (pseudosciaena crocea) were immunized by intraperitoneal injection. antibody response was assessed by method of enzyme-linked immunosorbent ... | 2007 | 17451968 |
| [distribution of thermostable direct hemolysin (tdh)- and tdh-related hemolysin (trh)-producing vibrio parahaemolyticus in coastal shimane prefecture and tdh and trh v parahaemolyticus contamination of retail shellfish]. | we studied distribution of thermostable direct hemolysin (tdh)- and tdh-related hemolysin (trh)-producing vibrio parahaemolyticus in coastal sea water, sediment, and shellfish and related retail shellfish contamination in shimane prefecture, japan, between 2002 and 2004. v. parahaemolyticus was isolated from > 80% of sea water, sediment, and shellfish. the detection of tdh gene (tdh) and trh gene (trh)-positive v parahaemolyticus in sea water was 11%, in sediment 16%, and in shellfish 26%. the n ... | 2007 | 17447472 |
| the dominance of pandemic serovars of vibrio parahaemolyticus in expatriates and sporadic cases of diarrhoea in thailand, and a new emergent serovar (o3 : k46) with pandemic traits. | vibrio parahaemolyticus is a major cause of gastroenteritis worldwide. a total of 95 v. parahaemolyticus isolates belonging to 23 different serovars were identified in a case-control study of expatriates and thai adults from 2001 to 2002 in thailand. fifty-two per cent of isolates (49/95) were resistant to ampicillin and sulfisoxazole, but all isolates were susceptible to ciprofloxacin and trimethoprim-sulfamethoxazole, two antibiotics commonly used to treat traveller's diarrhoea. all isolates w ... | 2007 | 17446282 |
| vibrio parahaemolyticus: a concern of seafood safety. | vibrio parahaemolyticus is a human pathogen that is widely distributed in the marine environments. this organism is frequently isolated from a variety of raw seafoods, particularly shellfish. consumption of raw or undercooked seafood contaminated with v. parahaemolyticus may lead to development of acute gastroenteritis characterized by diarrhea, headache, vomiting, nausea, and abdominal cramps. this pathogen is a common cause of foodborne illnesses in many asian countries, including china, japan ... | 2007 | 17418305 |
| scrg, a ggdef-eal protein, participates in regulating swarming and sticking in vibrio parahaemolyticus. | in this work, we describe a new gene controlling lateral flagellar gene expression. the gene encodes scrg, a protein containing ggdef and eal domains. this is the second ggdef-eal-encoding locus determined to be involved in the regulation of swarming: the first was previously characterized and named scrabc (for "swarming and capsular polysaccharide regulation"). ggdef and eal domain-containing proteins participate in the synthesis and degradation of the nucleotide signal cyclic di-gmp (c-di-gmp) ... | 2007 | 17400744 |
| modulation of pathogenicity with norepinephrine related to the type iii secretion system of vibrio parahaemolyticus. | norepinephrine (ne) controls the functions of the gastrointestinal tract, but its role in the pathogenicity of enteropathogens is not clear. we examined the effect of ne on the pathogenicity of vibrio parahaemolyticus with regard to its type iii secretion systems (ttsss). | 2007 | 17397007 |
| adrenergic regulation of bacterial virulence. | 2007 | 17396992 | |
| growth response of vibrio cholerae and other vibrio spp. to cyanobacterial dissolved organic matter and temperature in brackish water. | environmental control of growth and persistence of vibrios in aquatic environments is poorly understood even though members of the genus vibrio are globally important pathogens. to study how algal-derived organic matter and temperature influenced the abundance of different vibrio spp., baltic sea microcosms inoculated with vibrio cholerae, vibrio vulnificus, vibrio parahaemolyticus, vibrio alginolyticus and native bacterioplankton, were exposed to different temperatures (12-25 degrees c) and ame ... | 2007 | 17386033 |
| inactivation of foodborne pathogens using a one atmosphere uniform glow discharge plasma. | this study was conducted to determine the efficacy of a one atmosphere uniform glow discharge plasma (oaugdp) for inactivation of foodborne pathogens and to evaluate the influence of growth temperature, ph, and culture age on their inactivation. escherichia coli o157:h7, listeria monocytogenes, staphylococcus aureus, bacillus cereus, salmonella enteritidis, vibrio parahaemolyticus, yersinia enterocolitica, and shigella flexneri were evaluated. three-strain mixtures of each bacterium were inocula ... | 2007 | 17378708 |
| studies on the region involved in the transport activity of escherichia coli tolc by chimeric protein analysis. | gram-negative bacteria possess the outer membrane protein tolc which acts as an exit duct across the outer membrane. however, the region involved in the transport activity of tolc has remained unclear. we analyzed this region by creating chimeric tolcs. first, we expressed the genes for tolcs of vibrio parahaemolyticus (vp-tolc) and salmonella typhimurium (sal-tolc) in escherichia coli. the levels of sequence identity in the mature region of vp-tolc/ec-tolc and sal-tolc/ec-tolc with maximum matc ... | 2007 | 17350794 |
| comment on "vibrio vulnificus and v. parahaemolyticus necrotising fasciitis in fishermen visiting an estuarine tropical northern australia location". | 2007 | 17350104 | |
| variability of properties of vibrio parahaemolyticus strains isolated from individual patients. | infections by strains belonging to the o3:k6 pandemic clone of vibrio parahaemolyticus are prevalent in southern thailand, and serovariants of these strains have also been detected. v. parahaemolyticus strains lacking important virulence genes (tdh and trh) were isolated from 6.5 to 10.9% of clinical specimens during the period from 2000 to 2003. in order to understand whether changes to the characteristics of v. parahaemolyticus occur during infection, 10 isolates collected from each of 63 pati ... | 2007 | 17344357 |
| profile of acute immune response in chinese amphioxus upon staphylococcus aureus and vibrio parahaemolyticus infection. | amphioxus is traditionally considered as the living invertebrate most closely related to vertebrate. however, no systematic study was performed about how the amphioxus defends against the microbial invasion. here we reported a profile of gene transcription after staphylococcus aureus (s.c) and vibrio parahaemolyticus (v.p) challenged by suppression subtractive hybridization (ssh). when compared with mammals, amphioxus has the same acute immune defense genes (lectins, metalloproteinase, lysozymes ... | 2007 | 17343913 |
| expression, characterization and immunogenicity of a major outer membrane protein from vibrio alginolyticus. | vibrio alginolyticus is one of the vibrio pathogens common to humans and marine animals. during infection and induction of the host immune response, outer membrane proteins of bacteria play an important role. in this study, an outer membrane protein gene (ompw) was cloned from v. alginolyticus and expressed in escherichia coli. the 645 bp open reading frame (orf) encodes a protein of 214 amino acid residues with a predicted molecular weight of 23.3 kda. the amino acid sequence showed a high iden ... | 2007 | 17342258 |
| effects of heat-degraded sugars on survival and growth of vibrio parahaemolyticus and other bacteria. | we studied the effects of autoclaved (121 degrees c, 15 min) sugar solutions on the survival and growth of vibrio parahaemolyticus and other bacteria. the growth and survival of v. parahaemolyticus in luria-bertani media and phosphate buffer, respectively, were inhibited by the addition of d-glucose autoclaved in ph 8.0 phosphate buffer. the bactericidal effect of autoclaved d-glucose was very small when autoclaved in ph 7.0 phosphate buffer, but larger effects were observed when autoclaved in t ... | 2007 | 17340871 |
| evaluation of a tissue culture-based approach for differentiating between virulent and avirulent vibrio parahaemolyticus strains based on cytotoxicity. | the ability of only a subset of vibrio parahaemolyticus strains to cause human infection underscores the need for an analytical method that can effectively differentiate between pathogenic strains and those that do not cause disease. we tested the feasibility of a tissue culture-based assay to determine whether clinical isolates could be differentiated from nonclinical isolates based on relative isolate cytopathogenicity. to screen for cytotoxic capability, we measured relative extracellular lac ... | 2007 | 17340868 |
| purification and characterization of vibrio parahaemolyticus extracellular chitinase and chitin oligosaccharide deacetylase involved in the production of heterodisaccharide from chitin. | a chitin-degrading bacterial strain, kn1699, isolated from yatsu dry beach (narashino, chiba prefecture, japan), was identified as vibrio parahaemolyticus. treatment of powdered chitin with crude enzyme solution prepared from the supernatant of kn1699 cultures yielded a disaccharide, beta-d-n-acetylglucosaminyl-(1,4)-d-glucosamine (glcnac-glcn), as the primary chitin degradation product. the extracellular enzymes involved in the production of this heterodisaccharide, chitinase (pa-chi; molecular ... | 2007 | 17334758 |
| occurrence of vibrio spp. in blue mussels (mytilus edulis) from the german wadden sea. | the present study aimed at determining the contamination of blue mussels grown in germany with relevant bacterial organisms. seasonal variations were examined. for that purpose, mussel samples were taken regularly between june 2004 and may 2005 in seven shellfish-growing areas of the german wadden sea. a total of 90 samples were analysed. the analysis included the compulsory microbiological parameters escherichia coli and salmonella spp., as well as the determination of the contamination with vi ... | 2007 | 17331605 |
| o3:k6 serotype of vibrio parahaemolyticus identical to the global pandemic clone associated with diarrhea in peru. | to determine if the vibrio parahaemolyticus o3:k6 global pandemic clone has spread into peru. | 2007 | 17321179 |
| comparison of different primers for rapid detection of vibrio parahaemolyticus using the polymerase chain reaction. | the aim of this study was to compare different primers for rapid and effective detection of vibrio parahaemolyticus by polymerase chain reaction (pcr). | 2007 | 17309499 |
| isolation and characterization of pathogenic vibrio parahaemolyticus from diseased post-larvae of abalone haliotis diversicolor supertexta. | mass mortality among the post-larvae of cultured abalone haliotis diversicolor supertexta has occurred on the south coast of china since 2002. the diseased abalone are generally 10 to 30 days old, and typical signs of the disease include them turning white in colour and falling off the diatom films on which they were cultured. among sixteen different motile bacteria isolated from the diseased post-larvae, four were identified as vibrio parahaemolyticus on the basis of biochemical characteristics ... | 2007 | 17304623 |
| [classification and identification of vibrio cholerae and vibrio parahaemolyticus isolates based on gyrb gene phylogenetic analysis]. | in order to validate the usefulness of gyrb genotype for the classification and identification of vibrio cholerae and vibrio parahaemolyticus isolates, the phylogenetic analysis of 13 v. cholerae, 8 v. parahaemolyticus, 2 aeromonas hydrophila and 1 plesiomonas shigelloides strains was carried out using the partial coding sequence of gyrb, a gene that encodes the b subunit of dna gyrase (topoisomerase type ii ) in bacteria. these strains were separately clustered at species level and typed by the ... | 2006 | 17302148 |
| intussusception in a young female with vibrio gastroenteritis and diabetic ketoacidosis. | the incidence of functional intussusception is extremely rare in adults. a 23-year-old woman, previously diagnosed with type 1 diabetes mellitus (dm), complained of colicky abdominal pain associated with vomiting of 1-day duration. currant jelly stool was observed. irrespective of hydration and intravenous insulin injection under the diagnosis of diabetic ketoacidosis (dka), her abdominal pain and laboratory parameters did not improve. abdominal computerized tomography (ct) revealed a jejunojeju ... | 2007 | 17301511 |
| virulence gene- and pandemic group-specific marker profiling of clinical vibrio parahaemolyticus isolates. | vibrio parahaemolyticus is a halophilic bacterium capable of causing food- and waterborne gastroenteritis, wound infections, and septicemia in humans. the organism has recently received increasing attention, as the emergence of a new clone, v. parahaemolyticus o3:k6, has resulted in the first documented pandemic spread of v. parahaemolyticus. we used microarray analyses to explore the presence of known virulence factors and genetic markers thought to be specific for v. parahaemolyticus o3:k6 and ... | 2007 | 17301274 |
| sodium-independent low-affinity d-glucose transport by human sodium/d-glucose cotransporter 1: critical role of tryptophan 561. | although there is no evidence of significant na-independent glucose flux in tissues naturally expressing sglt1, previous kinetic and biophysical studies suggest that sodium/d-glucose cotransporter 1 (hsglt1) can facilitate sodium-independent d-glucose transport and may contain more than one sugar binding site. in this work, we analyze the kinetic properties and conformational states of isolated hsglt1 reconstituted in liposomes by transport and fluorescence studies in the absence of sodium. in t ... | 2007 | 17288452 |
| the role of vanadium in the chemical defense of the solitary tunicate, phallusia nigra. | ascidians (sea squirts) may defend themselves from predators, biofouling competitors, and bacterial infection by producing secondary metabolites or sequestering acid, but many species also accumulate heavy metals, most notably vanadium. the defensive functions of heavy metals in ascidians remain unclear, and to this end, the solitary caribbean tunicate, phallusia nigra, was studied to localize vanadium in its tissues and to assess the defensive properties of vanadium-containing compounds. as det ... | 2007 | 17265174 |
| [development of a predictive program for microbial growth under various temperature conditions]. | a predictive program for microbial growth under various temperature conditions was developed with a mathematical model. the model was a new logistic model recently developed by us. the program predicts escherichia coli growth in broth, staphylococcus aureus growth and its enterotoxin production in milk, and vibrio parahaemolyticus growth in broth at various temperature patterns. the program, which was built with microsoft excel (visual basic application), is user-friendly; users can easily input ... | 2006 | 17228795 |
| rapid detection and enumeration of trh-carrying vibrio parahaemolyticus with the alkaline phosphatase-labelled oligonucleotide probe. | an alkaline phosphatase (ap)-labelled oligonucleotide probe was developed to detect and enumerate trh(+)vibrio parahaemolyticus in seafood. the probe was evaluated using 40 isolates of v. parahaemolyticus, 45 isolates of other vibrios and 55 non-vibrio isolates. the probe reacted specifically with v. parahaemolyticus possessing either the trh1 or trh2 variant of the trh gene and was found to be 100% specific for trh(+)v. parahaemolyticus. using the trh probe, v. parahaemolyticus carrying trh gen ... | 2007 | 17227431 |
| global dissemination of vibrio parahaemolyticus serotype o3:k6 and its serovariants. | vibrio parahaemolyticus is recognized as a cause of food-borne gastroenteritis, particularly in the far east, where raw seafood consumption is high. an unusual increase in admissions of v. parahaemolyticus cases was observed at the infectious diseases hospital in calcutta, a city in the northeastern part of india, beginning february 1996. analysis of the strains revealed that a unique serotype, o3:k6, not previously isolated during the surveillance in calcutta accounted for 50 to 80% of the infe ... | 2007 | 17223622 |
| vibrio alginolyticus infections in the netherlands after swimming in the north sea. | 2006 | 17213549 | |
| inhibitory activity of thai condiments on pandemic strain of vibrio parahaemolyticus. | antibacterial activity of 13 condiments used in thai cooking was investigated with a pandemic strain of vibrio parahaemolyticus. using a disk diffusion technique, freshly squeezed extracts from galangal, garlic and lemon, at a concentration of 10 microl/disk produced a clear zone of 13.6+/-0.5, 11.6+/-0.5 and 8.6+/-1.2mm, respectively. the inhibitory activity of these 3 condiments on pandemic strains was not significantly different from that on non-pandemic strains of v. parahaemolyticus. becaus ... | 2007 | 17189767 |
| involvement of radical species in inactivation of vibrio parahaemolyticus in saline solutions by direct-current electric treatment. | the effect of pulsed low-direct-current (dc) electric treatment on the viability of vibrio parahaemolyticus in artificial seawater and 3.0% (w/v) nacl solution was studied as a function of available chlorine (ac) concentration. the amount of ac generated during the dc electric treatment increased in proportion to the amount of passed dc. the survival fraction of v. parahaemolyticus cells decreased depending on ac concentration. when the generated ac components were completely reduced in the pres ... | 2006 | 17189175 |
| use of linear, weibull, and log-logistic functions to model pressure inactivation of seven foodborne pathogens in milk. | survival curves of six foodborne pathogens suspended in ultra high-temperature (uht) whole milk and exposed to high hydrostatic pressure at 21.5 degrees c were obtained. vibrio parahaemolyticus was treated at 300 mpa and other pathogens, listeria monocytogenes, escherichia coli o157:h7, salmonella enterica serovar enteritidis, salmonella enterica serovar typhimurium, and staphylococcus aureus were treated at 600 mpa. all the survival curves showed a rapid initial drop in bacterial counts followe ... | 2007 | 17188197 |
| comparison of different biochemical and molecular methods for the identification of vibrio parahaemolyticus. | multicentre evaluation of biochemical and molecular methods for the identification of vibrio parahaemolyticus. | 2007 | 17184339 |
| rapid detection of vibrio species using liquid microsphere arrays and real-time pcr targeting the ftsz locus. | the development of rapid and sensitive molecular techniques for the detection of vibrio species would be useful for the surveillance of sporadic infections and management of major outbreaks. comparative sequence analysis of the ftsz gene in the predominant vibrio species that cause human disease revealed distinct alleles for each examined species, including vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus. light upon extension (lux) real-time pcr assays were developed to target the ... | 2007 | 17172518 |
| a pulsed-field gel electrophoresis typing scheme for vibrio parahaemolyticus isolates from fifteen countries. | vibrio parahaemolyticus is an important foodborne pathogen in taiwan and many other maritime asian countries where seafood is frequently consumed. a total of 535 strains of v. parahaemolyticus were recovered mostly (97%) from clinical samples obtained in taiwan or in 14 other countries. these strains were typed by pulsed-field gel electrophoresis following sfii digestion and a typing scheme was generated. the 115 different patterns identified were grouped into 13 types with dissimilarity values ... | 2007 | 17161487 |
| evaluation of an alkaline phosphatase-labeled oligonucleotide probe for the detection and enumeration of the thermostable-related hemolysin (trh) gene of vibrio parahaemolyticus. | reliable methods are needed to detect total and pathogenic vibrio parahaemolyticus. one marker of v. parahaemolyticus virulence is the thermostable-related hemolysin. we developed an alkaline phosphatase-labeled dna probe method for the specific detection and enumeration of trh-positive v. parahaemolyticus by colony hybridization. the probe was tested against a panel of 200 bacterial strains and determined to be specific for trh-positive v. parahaemolyticus. additionally, the trh alkaline phosph ... | 2006 | 17133826 |
| occurrence and density of vibrio parahaemolyticus in live edible crustaceans from markets in china. | vibrio parahaemolyticus is a marine bacterium causing foodborne disease. occurrence of the bacterium was investigated in six species of edible crustaceans available from markets in mainland china. the bacterium was detected in 22 of 45 whole-body, shell, and feces samples, including mitten crabs, which are supposed to be produced in freshwater ponds. the mean densities ranged from 2.8 log cfu/g in mitten crabs (eriocheir sinensis) to 5.1 cfu/g in giant tiger prawns (penaeus monodon). in hemolymp ... | 2006 | 17133820 |
| distribution of virulent and pandemic strains of vibrio parahaemolyticus in three molluscan shellfish species (meretrix meretrix, perna viridis, and anadara granosa) and their association with foodborne disease in southern thailand. | distribution of pandemic strains of vibrio parahaemolyticus in seafood, particularly in molluscan shellfish, and their serological and molecular relationships to clinical strains were examined from hat yai city in southern thailand. during 2000 to 2002, virulent strains (tdh+ or trh+) were isolated from 13 of 230 molluscan shellfish samples using alkaline peptone water enrichment followed by immunomagnetic separation. the isolates included 12 pandemic strains (tdh+, trh-, group-specific pcr posi ... | 2006 | 17133804 |
| analysis of bacteria degradation products of methyl parathion by liquid chromatography/electrospray time-of-flight mass spectrometry and gas chromatography/mass spectrometry. | the biodegradation of the organophosphorus insecticide methyl parathion (mp) in aqueous environment by bacteria isolated from river sediment has been studied. two species of bacteria which show strong mp degradation ability are identified as shewanella and vibrio parahaemolyticus. the biodegradation of mp proceeded rapidly with the formation of a series of intermediate products, which were analyzed using a combination of gc/ms and hplc/esi-tofms techniques. the major products tentatively identif ... | 2006 | 17126847 |
| incidence of enteric bacterial pathogens in water found at the bottom of commercial freezers in calabar, southeastern nigeria. | bacteriological analysis of water that accumulates at the bottom of freezers in restaurants when the power was cut in calabar, southeastern nigeria, was carried out using standard procedures. mean heterotrophic bacterial counts and escherichia coli counts ranged from 3.1 +/- 0.02 to 7.1 +/- 0.30 x 10(4) cfu/ml and 0.2 +/- 0.10 to 0.6 +/- 0.50 x 10(4) cfu/ml, respectively, indicating heavy bacterial contamination whose source was mostly fecal. there was no significant difference (p > 0.05, 0.01) ... | 2006 | 17125005 |
| involvement of the vibrio parahaemolyticus pvsc gene in export of the siderophore vibrioferrin. | the pvsc gene of unknown function has been found in the iron-regulated vibrioferrin biosynthesis operon of vibrio parahaemolyticus (tanabe, t. et al., j. bacteriol. 185: 6938-6949, 2003). the amino acid sequence deduced from the gene showed significant similarity to 12-transmembrane segment efflux pumps belonging to the major facilitator superfamily. a nonpolar deletion of pvsc in v. parahaemolyticus resulted in a reduced release of vibrioferrin into the medium. vibrioferrin release could be reg ... | 2006 | 17116982 |
| response of bdellovibrio and like organisms (balos) to the migration of naturally occurring bacteria to chemoattractants. | a dual culture-based and non-culture-based approach was applied to characterize predator bacterial groups in surface water samples collected from apalachicola bay, florida. chemotaxis drop assays were performed on concentrated samples in an effort to isolate predator bacteria by their chemotactic ability. yeast extract (ye) and casamino acids (ca) proved to be strong chemoattractants and resulted in three visibly distinct bands; however, dextrose, succinate, pyruvate, and concentrated cells of v ... | 2006 | 17115104 |
| outbreak of gastroenteritis caused by the pandemic vibrio parahaemolyticus o3:k6 in mexico. | during 2003 and during late september of 2004, more than 1230 cases of gastroenteritis were reported in the south of sinaloa state, north-western mexico. all cases were attributed to the consumption of raw or undercooked shrimp collected at the huizache-caimanero lagunary system. vibrio parahaemolyticus was identified by standard biochemical methods, and many strains were positive for pcr amplifications of the tlh and tdh genes and negative for the trh gene. a representative strain belonged to t ... | 2006 | 17107421 |
| identification of vibrio isolates by a multiplex pcr assay and rpob sequence determination. | vibrio, a diverse genus of aquatic bacteria, currently includes 72 species, 12 of which occur in human clinical samples. of these 12, three species--vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus-account for the majority of vibrio infections in humans. rapid and accurate identification of vibrio species has been problematic because phenotypic characteristics are variable within species and biochemical identification requires 2 or more days to complete. to facilitate the identifi ... | 2007 | 17093013 |
| efficacy of electrolysed oxidizing water in inactivating vibrio parahaemolyticus on kitchen cutting boards and food contact surfaces. | to determine the efficacy of electrolysed oxidizing (eo) water in inactivating vibrio parahaemolyticus on kitchen cutting boards and food contact surfaces. | 2006 | 17083714 |
| high amount of dinophysistoxin-3 in mytilus chilensis collected in seno de reloncaví, chile, during massive human intoxication associated with outbreak of vibrio parahaemolyticus. | this study describes the detection of high amount of 7-o-acyl-derivative dinophysistoxin-1 (dinophysistoxin-3) in filter bivalves collected on february 2005 in the seno de reloncaví, puerto montt city, southern chile, in the same period of time where an intoxication episode was associated with the presence of vibrio parahaemolyticus in shellfish. the diarrhetic shellfish poisoning (dsp) mouse bioassay of mussel extract samples, performed as described for regulatory testing, were negative to dsp ... | 2006 | 17077585 |
| antibacterial spectrum of plant polyphenols and extracts depending upon hydroxyphenyl structure. | the relationship between the structure and antibacterial activity of 22 polyphenols was analyzed by using minimum inhibitory concentration (mic) as a criterion against 26 species of bacteria which can grow in mueller-hinton medium. there was no clear correlation between gram-staining and bacterial susceptibility to polyphenols, and the extent of the susceptibility was approximately dependent on the species of bacteria. in the same gram-negative bacteria, the antibacterial activity of the polyphe ... | 2006 | 17077519 |
| characterization of a vibrio alginolyticus strain, isolated from alaskan oysters, carrying a hemolysin gene similar to the thermostable direct hemolysin-related hemolysin gene (trh) of vibrio parahaemolyticus. | a vibrio strain isolated from alaskan oysters and classified by its biochemical characteristics as vibrio alginolyticus possessed a thermostable direct hemolysin-related hemolysin (trh) gene previously reported only in vibrio parahaemolyticus. this trh-like gene was cloned and sequenced and was 98% identical to the trh2 gene of v. parahaemolyticus. this gene seems to be functional since it was transcriptionally active in early-stationary-phase growing cells. to our knowledge, this is the first r ... | 2006 | 17056701 |
| rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time pcr. | buoyant density gradient centrifugation has been used to separate bacteria from complex food matrices, as well as to remove compounds that inhibit rapid detection methods, such as pcr, and to prevent false-positive results due to dna originating from dead cells. applying a principle of buoyant density gradient centrifugation, we developed a method for rapid separation and concentration following filtration and low- and high-speed centrifugation, as well as flotation and sedimentation buoyant den ... | 2007 | 17056684 |
| tetrameric structure of thermostable direct hemolysin from vibrio parahaemolyticus revealed by ultracentrifugation, small-angle x-ray scattering and electron microscopy. | the thermostable direct hemolysin (tdh) is a major virulence factor of vibrio parahaemolyticus. we have characterized the conformational properties of tdh by small-angle x-ray scattering (saxs), ultracentrifugation and transmission electron microscopy. sedimentation equilibrium and velocity studies revealed that the protein is tetrameric in aqueous solvents. the guinier plot derived from saxs data provided a radius of gyration of 29.0 a. the elongated pattern with a shoulder of a pair distance d ... | 2007 | 17056060 |
| two type iv pili of vibrio parahaemolyticus play different roles in biofilm formation. | vibrio parahaemolyticus rimd2210633 has two sets of type iv-a pilus genes. one set is similar to that found in other gram-negative bacteria, such as pseudomonas aeruginosa, vibrio cholerae (chitin-regulated pilus; chirp), and vibrio vulnificus. the other is homologous to the genes for the mannose-sensitive hemagglutinin (msha) pilus. in this study, we analyzed the effects of the deletions in the pilin genes for each type iv pilus (the chirp and the msha pilus) on biofilm formation. although the ... | 2006 | 17020553 |
| vp1686, a vibrio type iii secretion protein, induces toll-like receptor-independent apoptosis in macrophage through nf-kappab inhibition. | vibrio parahaemolyticus, causative agent of human gastrointestinal diseases, possesses several virulent machineries including thermostable direct hemolysin and type iii secretion systems (ttss1 and -2). in this report, we establish that ttss1-dependent secretion and translocation of a v. parahaemolyticus effector protein vp1686 into the cytosol induces dna fragmentation in macrophages. we performed yeast two-hybrid screening to identify the molecules involved in vp1686-mediated cell death pathwa ... | 2006 | 16984916 |
| bacterial lateral flagella: an inducible flagella system. | flagella are complex surface organelles that allow bacteria to move towards favourable environments and that contribute to the virulence of pathogenic bacteria through adhesion and biofilm formation on host surfaces. there are a few bacteria that possess functional dual flagella systems, such as vibrio parahaemolyticus, some mesophilic aeromonas spp., rhodospirillum centenum and azospirillum brasilense. these bacteria are able to express both a constitutive polar flagellum required for swimming ... | 2006 | 16978346 |
| response of spinach and komatsuna to biogas effluent made from source-separated kitchen garbage. | recycling of kitchen garbage is an urgent task for reducing public spending and environmental burdens by incineration and/or landfill. there is an interesting regional effort in ogawa, saitama prefecture, japan, in which source-separated kitchen garbage is anaerobically fermented with a biogas plant and the resultant effluent is used as a quick-release organic fertilizer by surrounding farmers. however, scientific assessments of fertilizer values and risks in the use of the effluent were lacking ... | 2006 | 16973635 |
| development of monoclonal antibodies for simple identification of vibrio alginolyticus. | the present study was aimed to produce monoclonal antibodies (mabs) for simple and specific identification of vibrio alginolyticus infection in shrimp. | 2006 | 16965376 |
| preparation and use of media for protease-producing bacterial strains based on by-products from cuttlefish (sepia officinalis) and wastewaters from marine-products processing factories. | cuttlefish powder (cfp) from sepia officinalis by-products was prepared and tested as a fermentation substrate for microbial growth and protease production by several species of bacteria: bacillus licheniformis, bacillus subtilis, pseudomonas aeruginosa, bacillus cereus bg1, and vibrio parahaemolyticus. all microorganisms studied grew well and produced protease activity when cultivated in medium containing only cfp indicating that the strains can obtain their carbon and nitrogen source requireme ... | 2008 | 16962303 |
| rapid estimation of microbial numbers in water using bulk fluorescence. | enumeration of microbial cells without culturing is an essential technique for microbial ecology and water quality evaluation. here we show that bulk fluorescence using the sybr gold dna stain can be used to rapidly quantify microbial cells per millilitre in fresh, marine and estuarine waters. the bulk fluorescence method is comparable to estimating cell concentrations in cultures using optical density; however, this enhanced method enables the user to estimate microbial numbers at lower concent ... | 2006 | 16958758 |
| quorum sensing-disrupting brominated furanones protect the gnotobiotic brine shrimp artemia franciscana from pathogenic vibrio harveyi, vibrio campbellii, and vibrio parahaemolyticus isolates. | autoinducer 2 (ai-2) quorum sensing was shown before to regulate the virulence of vibrio harveyi towards the brine shrimp artemia franciscana. in this study, several different pathogenic v. harveyi, vibrio campbellii, and vibrio parahaemolyticus isolates were shown to produce ai-2. furthermore, disruption of ai-2 quorum sensing by a natural and a synthetic brominated furanone protected gnotobiotic artemia from the pathogenic isolates in in vivo challenge tests. | 2006 | 16957276 |
| visible-light-induced bactericidal activity of a nitrogen-doped titanium photocatalyst against human pathogens. | the antibacterial activity of photocatalytic titanium dioxide (tio(2)) substrates is induced primarily by uv light irradiation. recently, nitrogen- and carbon-doped tio(2) substrates were shown to exhibit photocatalytic activities under visible-light illumination. their antibacterial activity, however, remains to be quantified. in this study, we demonstrated that nitrogen-doped tio(2) substrates have superior visible-light-induced bactericidal activity against escherichia coli compared to pure t ... | 2006 | 16957236 |
| response of vibrio parahaemolyticus to ethanol shock. | in this study, the growth and survival of vibrio parahaemolyticus in the presence of 0.0-8.0% ethanol was first examined. v. parahaemolyticus was then exposed to a sub-lethal dose of 5.0% ethanol for 30 and 60 min (ethanol shock). morphological changes and alterations in cell leakage, thermal tolerance at 47 degrees c, and susceptibility to 8% ethanol and low temperature (4 and -18 degrees c) of v. parahaemolyticus caused by ethanol shock were investigated. in addition, recoveries of the ethanol ... | 2006 | 16943038 |