Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| pathogenesis and treatment of hepatitis e virus infection. | hepatitis e has been considered to be a travel-associated, acute, self-limiting liver disease that causes fulminant hepatic failure in specific high-risk groups only. however, hepatitis e virus (hev) infection can also be acquired in industrialized countries-hev genotype 3 infection is a zoonosis, with pigs and rodents serving as animal reservoirs. in recent years, cases of chronic hev infection that were associated with progressive liver disease have been described in several cohorts of immunoc ... | 2012 | 22537448 |
| cross-species infection of pigs with a novel rabbit, but not rat, strain of hepatitis e virus isolated in the united states. | hepatitis e virus (hev) is an important human pathogen. in addition to humans, hev has also been identified in pig, chicken, mongoose, deer, rat, rabbit and fish. there are four recognized and two putative genotypes of mammalian hev. genotypes 1 and 2 are restricted to humans, while genotypes 3 and 4 are zoonotic. the recently identified rabbit hev is a distant member of genotype 3. here, we first expressed and purified the recombinant capsid protein of rabbit hev and showed that the capsid prot ... | 2012 | 22535776 |
| prevalence and transmission of hepatitis e virus in domestic swine populations in different european countries. | hepatitis e virus (hev) genotype 3 and 4 can cause liver disease in human and has its main reservoir in pigs. hev investigations in pigs worldwide have been performed but there is still a lack of information on the infection dynamics in pig populations. | 2012 | 22534364 |
| low risk of hepatitis e among dutch short-term travelers. | hepatitis e is endemic in (sub)tropical countries while only sporadic cases have been described in industrialized countries. in a prospective study among 1270 short-term dutch travelers to (sub)tropical countries we found no seroconversion to anti-hepatitis e virus (hev) antibodies, indicating a very low risk for travelers to acquire a hepatitis e infection. | 2012 | 22530832 |
| hepatitis a, b, c and e virus markers in chinese residing in tokyo, japan. | recently, the number of foreigners living in japan has been increasing, with the majority originating from china. it is important for us to know the prevalence of hepatitis virus markers among them, as proper medical practices and vaccinations should be prepared when seeing them and their offspring. | 2012 | 22524475 |
| hepatitis e virus infection among solid organ transplant recipients, the netherlands. | we screened 1,200 living heart, lung, liver, and kidney transplant recipients for hepatitis e virus infection by reverse transcription pcr. in 12 (1%) patients, hepatitis e virus infection was identified; in 11 patients, chronic infection developed. this immunocompromised population is at risk for hepatitis e virus infection. | 2012 | 22516170 |
| prevalence of hepatitis e virus antibodies in individuals exposed to swine in mato grosso, brazil. | this study was conducted to estimate the prevalence of hepatitis e antibodies (anti-hev) among individuals exposed to swine in the rural areas of the state of mato grosso (mt) in brazil. the study included 310 participants who had an average age of 39 years. fifty-one per cent of the participants were female and 26 (8.4%) were anti-hev-positive. concomitantly, we studied 101 blood donors from the urban area of the state capital who had never lived in a rural area or handled swine. four per cent ... | 2012 | 22510828 |
| hepatitis e: a complex and global disease. | thirty years after its discovery, the hepatitis e virus (hev) continues to represent a major public health problem in developing countries. in developed countries, it has emerged as a significant cause of non-travel-associated acute hepatitis. hev infects a wide range of mammalian species and a key reservoir worldwide appears to be swine. genomic sequence similarity between some human hev genotypes and swine hev strains has been identified and we know that humans can acquire hev infection from a ... | 2008 | 22460217 |
| prevalence of hepatitis e virus in swine fed on kitchen residue. | the aim of this study was to investigate the prevalence of swine hepatitis e virus (hev) in pigs fed different feedstuffs (kitchen residue or mixed feeds) and genetic identification of hev isolated in hebei province, china. serum and fecal samples were collected from adult swine. anti-hev antibody was evaluated by double sandwich antigen enzyme immunoassay. hev rna was extracted from fecal samples and amplified by nested rt-pcr. the reaction products were sequenced, and the sequence analyzed. vi ... | 2012 | 22457765 |
| identical consensus sequence and conserved genomic polymorphism of hepatitis e virus during controlled interspecies transmission. | high-throughput sequencing of bile and feces from two pigs experimentally infected with human hepatitis e virus (hev) of genotype 3f revealed the same full-length consensus sequence as in the human sample. twenty-nine percent of polymorphic sites found in hev from the human sample were conserved throughout the infection of the heterologous host. the interspecies transmission of hev quasispecies is the result of a genomic negative-selection pressure on random mutations which can be deleterious to ... | 2012 | 22457521 |
| hepatitis e virus-associated aplastic anaemia: the first case of its kind. | here we report the case of a 32-year-old pakistani male, who developed severe aplastic anaemia after a severe attack of acute hepatitis e virus (hev) infection. his laboratory test values were not in normal ranges. the liver enzymes were elevated. serologic and/or molecular-based tests for hepatitis a, b, c, d, g, transfusion transmitted virus (ttv) and b19 were negative, whereas anti-hev igm and hev rna polymerase chain reaction (pcr) was also detected in the patient sample. the patient receive ... | 2012 | 22441030 |
| the psap motif within the orf3 protein of an avian strain of the hepatitis e virus is not critical for viral infectivity in vivo but plays a role in virus release. | the orf3 protein of hepatitis e virus (hev) is a multifunctional protein important for virus replication. the orf3 proteins from human, swine, and avian strains of hev contain a conserved pxxp amino acid motif, resembling either src homology 3 (sh3) cell signaling interaction motifs or "late domains" involved in host cell interactions aiding in particle release. using an avian strain of hev, we determined the roles of the conserved prolines within the prepsapp motif in hev replication and infect ... | 2012 | 22438540 |
| hepatitis e virus replication requires an active ubiquitin-proteasome system. | the mechanism of hepatitis e virus (hev) replication remains largely unknown. here we demonstrate that hev replication requires an active ubiquitin-proteasome system and that proteasome inhibitors affect hev replication, possibly by inhibition of viral transcription or/and translation without a significant effect on cellular translation. overexpression of ubiquitin in inhibitor-treated cells partially reverses the inhibitor effect on hev replication. the results suggest that hev replication requ ... | 2012 | 22438536 |
| detection of human food-borne and zoonotic viruses on irrigated, field-grown strawberries. | this study evaluated the presence of pathogenic human and zoonotic viruses on irrigated, field-grown strawberries. norovirus genogroup i, rotavirus, and swine hepatitis e virus genogroup 3 were detected on strawberries, and irrigation water is suspected as the contamination origin. | 2012 | 22427499 |
| low risk of hepatitis e virus reactivation after haematopoietic stem cell transplantation. | hepatitis e virus (hev) is an emerging cause of acute hepatitis in industrialized countries. hev infection can evolve to chronic hepatitis in immunocompromised patients. additionally, hev reactivation after haematopoietic stem cell transplantation has been reported. | 2012 | 22425538 |
| serological and molecular study of hepatitis e virus among illegal blood donors. | to investigate the seroprevalence and molecular characteristics of hepatitis e virus (hev) in the illegal blood donors (ibds) of central china in the early 1990s. | 2012 | 22408360 |
| genetic characteristics and pathogenicity of human hepatitis e virus in nanjing, china. | to investigate the genetic characteristics and pathogenicity of hepatitis e virus (hev) and assess the potential risk factors for sporadic hepatitis e. | 2012 | 22408357 |
| circulation of genotype 4 hepatitis e virus in europe: first autochthonous hepatitis e infection in france. | human hev infections reported in europe without previous travel to endemic regions are linked to exposure to genotype 3 hepatitis e virus (hev).genotype 3 is widely distributed through human cases and zoonotic reservoir. the geographical distribution of genotype 4 is limited to asian countries. | 2012 | 22405947 |
| a retrospective study on imported hepatitis e in japan. | hepatitis e virus (hev), a causative agent of human hepatitis e, is transmitted through an oral-fecal route, often by contaminated drinking water. serum specimens were collected from 112 non-a, -b, and -c acute hepatitis patients from 1989 to 2004 in japan. of these, 24 patients were found to be positive for anti-hev igm and diagnosed with acute hepatitis e. seventeen of these patients had a clear history of traveling abroad before disease onset and were counted as cases of imported hev infectio ... | 2012 | 22405678 |
| [hepatitis e: an emerging disease]. | the hepatitis e virus is endemic in countries with poor sanitation, where it has many similarities with the hepatitis a virus. it causes a strictly human, feco-oral transmitted, acute, self-limited hepatitis in young adults. the outcome is excellent, except in pregnant women and cirrhotic patients, who experience a high mortality rate. the first cases described in industrialized countries were travellers coming from endemic areas. however, there is now growing evidence that locally-acquired hepa ... | 2012 | 22405325 |
| seroprevalence of hepatitis e virus infection in rural and urban populations, tunisia. | hepatitis e virus (hev) is one of the leading agents of acute hepatitis. this study investigated the prevalence and risk factors of hev infection in the tunisian adult general population, either in blood donors (n=687) or in patients hospitalized for acute hepatitis (n=202). the mode of transmission differed between these two populations: contact with animals and living in a rural habitat were the main risk factors for being in contact with hev in asymptomatic blood donors, while hev was contrac ... | 2012 | 22404115 |
| first human case of co-infection with two different subtypes of hepatitis e virus. | hepatitis e virus (hev), the main etiologic agent of enterically transmitted acute hepatitis in developing countries, is now recognized as an emerging agent of autochthonous disease and chronic hepatitis e in immunocompromised patients in europe where hev infection is probably zoonotically acquired. we describe the first human case of acute hev infection with two genotype 3 viruses in a french kidney transplant recipient, probably acquired through consumption of uncooked pig liver sausage (figat ... | 2012 | 22398950 |
| adaptation of a genotype 3 hepatitis e virus to efficient growth in cell culture depends on an inserted human gene segment acquired by recombination. | an infectious cdna clone of a genotype 3 strain of hepatitis e virus adapted to growth in hepg2/c3a human hepatoma cells was constructed. this virus was unusual in that the hypervariable region of the adapted virus contained a 171-nucleotide insertion that encoded 58 amino acids of human s17 ribosomal protein. analyses of virus from six serial passages indicated that genomes with this insert, although initially rare, were selected during the first passage, suggesting it conferred a significant g ... | 2012 | 22398290 |
| [hepatitis e: molecular virology, epidemiology and pathogenesis]. | hepatitis e represents a significant proportion of enteric transmitted liver diseases and poses a major public health problem, mainly associated with epidemics due to contamination of water supplies, especially in developing countries. hepatitis e virus (hev) is responsible for self-limiting acute liver oral-faecal infections. in industrialised countries, acute hepatitis e is sporadic, detected in travellers from endemic areas but also in sporadic cases with no risk factors. hev is a non-envelop ... | 2012 | 22386306 |
| cytokine profiles, ctl response and t cell frequencies in the peripheral blood of acute patients and individuals recovered from hepatitis e infection. | hepatitis e is a major public health problem in the developing countries. pathogenesis of hepatitis e virus (hev) infection is poorly understood. | 2012 | 22384080 |
| giv noroviruses and other enteric viruses in bivalves: a preliminary study. | we evaluated the presence of the enteric viruses: norovirus, adenovirus, enterovirus, astrovirus, hepatitis a virus, and hepatitis e virus in bivalves using nested pcr methods and cell culture assays. noroviruses gii.4 and giv.1, adenoviruses types 1 and 2, hepatitis a, and echovirus type 7 were detected in the shellfish tested, which were often co-infected. this is the first study to detect such a high level of viral contamination in italian mussels (up to four different viral groups in a singl ... | 2012 | 22378550 |
| hepatitis e virus infection in hiv-infected persons. | to determine whether hepatitis e virus (hev) is a cause of hepatitis among hiv-infected persons, we evaluated 1985-2009 data for us military beneficiaries. evidence of acute or prior hev infection was detected for 7 (4%) and 5 (3%) of 194 hiv-infected persons, respectively. hev might be a cause of acute hepatitis among hiv-infected persons. | 2012 | 22377220 |
| prolonged jaundice attributed to super infection of hepatitis e virus in a case of resolving leptospirosis. | india is endemic for both leptospira and hepatitis e virus (hev). the clinical presentations of these diseases have overlapping features. we report a case of superinfection of hev in a patient with resolving leptospirosis with underlying hodgkin lymphoma. the diagnosis of hev in our case was established by hev-rna pcr as our patient was immunosuppressed. the present study highlights the need for molecular diagnosis in the case of hev infection with strong clinical suspicion and negative serologi ... | 2012 | 22361773 |
| cirrhosis due to chronic hepatitis e infection in a child post-bone marrow transplant. | chronic hepatitis e virus (hev) infection occurs in immunosuppressed adults. we detected hev ribonucleic acid in serum of an adolescent patient who had undergone bone marrow transplantation and subsequently presented with persistently increased aminotransferases and histologic chronic hepatitis, and eventually developed cirrhosis. phylogenetic analysis revealed these hev strains were similar to swine genotype 3a, suggesting a possible zoonosis. | 2012 | 22341950 |
| first report of acute autochthonous hepatitis e in portugal. | hepatitis e infection is usually a self-limiting disease. in industrialized countries, sporadic cases of acute hepatitis e virus (hev) infections have been described; their number seems to be increasing in european countries. we report the first human case of autochthonous acute hepatitis e confirmed in portugal. patients with acute non-a-c hepatitis should be tested for hev in portugal and hepatitis e infection should be considered in the differential diagnosis of unexplained hepatitis cases. | 2012 | 22337852 |
| first case report of an acute genotype 3 hepatitis e infected pregnant woman living in south-eastern france. | in european countries, epidemiology of hepatitis e virus (hev) infection is not well known. although, seroprevalence of hev immunoglobulin g reached a few percent in european women, no acute hepatitis e during pregnancy has been described so far. here, we report a case of an autochthonous hev genotype 3 infection in a 41-years-old pregnant woman living in a non-endemic country. the acute hepatitis had a spontaneous good outcome for the mother and the child. in non-endemic areas where hepatitis e ... | 2012 | 22336086 |
| acute pancreatitis in acute viral hepatitis. | the association of acute viral hepatitis and acute pancreatitis is well described. this study was conducted to find out the frequency of pancreatic involvement in acute viral hepatitis in the nepalese population. | 2011 | 22335088 |
| coinfection of hepatitis a virus genotype ia and iiia complicated with autoimmune hemolytic anemia, prolonged cholestasis, and false-positive immunoglobulin m anti-hepatitis e virus: a case report. | a 37-year-old male presented with fever and jaundice was diagnosed as hepatitis a complicated with progressive cholestasis and severe autoimmune hemolytic anemia. he was treated with high-dose prednisolone (1.5 mg/kg), and eventually recovered. his initial serum contained genotype ia hepatitis a virus (hav), which was subsequently replaced by genotype iiia hav. moreover, at the time of development of hemolytic anemia, he became positive for immunoglobulin m (igm) anti-hepatitis e virus (hev). we ... | 2011 | 22310798 |
| author's reply: hepatitis e virus infection in iranian kidney-transplant patients. | 2011 | 22308160 | |
| hepatitis e virus infection in iranian kidney-transplant patients. | 2011 | 22308159 | |
| hev-capsid protein interacts with cytochrome p4502c8 and retinol-binding protein 4. | hepatitis e virus (hev) is a major causative agent of acute clinical hepatitis in adults throughout much of asia, the middle east, and africa. the lack of an efficient cell culture system for hev has greatly limited our understanding of the mechanisms of infection, replication, and pathogenicity of this virus. the yeast two-hybridization system is considered to be an efficient method for determining protein-protein interactions and screening interactive proteins associated with host cells. | 2011 | 22308156 |
| a look at the past history of hepatitis e in haiti: should it be a warning sign during the current crisis? | nobody can forget the devastating 7.0magnitude earthquake that struck poverty-stricken haiti, port-au-prince recently on 12 january 2010. at least 75,000 people were killed and hundreds of thousands became homeless; authorities are worried about sanitation and outbreaks of disease in the region. the camps are full of people and there are not even the most basic facilities for any others. humanity obliges us to help them in any possible way. i reviewed the literature about the hepatitis e virus i ... | 2010 | 22308118 |
| phylogenetic analysis of 626 hepatitis e virus (hev) isolates from humans and animals in china (1986-2011) showing genotype diversity and zoonotic transmission. | hepatitis e is considered as a public health problem in china. to determine the overall molecular epidemiology of hepatitis e virus (hev) and analyze the situation of cross-species transmission between humans and swine in china over the last 25 years (1986-2011), 626 hev complete and partial sequences (89 isolates identified by our group) isolated from humans and animals in china were retrieved from genbank and subjected to phylogenetic analysis. there were three genotypes and 11 sub-genotypes o ... | 2012 | 22306814 |
| hepatitis e virus infection: more common than previously realized? | 2011 | 22298973 | |
| hepatitis e virus and the kidney in solid-organ transplant patients. | hepatitis e virus (hev) infection is an emerging disease in industrialized countries. few data regarding genotype 3 hev extrahepatic manifestations exist. | 2012 | 22298032 |
| prevalence of antibodies and rna genome of hepatitis e virus in a cohort of french immunocompromised. | recently, cases of chronic hepatitis e have been identified in immunocompromised patients. | 2012 | 22293627 |
| pleconaril revisited: clinical course of chronic enteroviral meningoencephalitis after treatment correlates with in vitro susceptibility. | human enteroviruses (hevs) can cause severe infections, especially in patients with a deficient humoral immune response, such as x-linked agammaglobulinemia. in this patient group, chronic enteroviral meningitis (cema) is feared because of extensive morbidity and high fatality rate. treatment options consist of intravenous immunoglobulin (ivig), with various outcomes. pleconaril is an antiviral agent with in vitro activity against hevs that has been used in the treatment of hev infections. | 2011 | 22293148 |
| guillain-barré syndrome associated with acute hepatitis e infection. | guillain-barré syndrome (gbs) is a neurological emergency that warrants prompt diagnosis and treatment. occasionally, the clinical, laboratory and electrophysiological features are neither typical nor diagnostic. it requires a high index of suspicion and clinical judgement for early diagnosis and commencement of treatment in those scenarios. some pathogens are potential triggers of this serious neurological disease, including: campylobacter jejuni, mycoplasma pneumoniae and cytomegalovirus. alth ... | 2012 | 22285113 |
| seroprevalence of hepatitis e antibodies and risk profile of hev seropositivity in the netherlands, 2006-2007. | summaryhepatitis e virus (hev) is now considered an endemic pathogen in industrialized countries, leading to acute and sometimes chronic hepatitis, mostly in vulnerable people. the endemic sources are unclear. a survey in the netherlands in 2006-2007 showed a 1·9% seroprevalence of hev antibodies measured by elisa and confirmed by immunoblot in a nationwide sample. overall, in 134/7072 (1·9%) seropositive individuals, older age (p<0·01), being male (p<0·01), working with patients (p=0·03), worki ... | 2012 | 22269886 |
| systematic screening for novel, serologically reactive hepatitis e virus epitopes. | the national institutes of health classified hepatitis e as an emerging disease since hepatitis e virus (hev) is the major cause of acute hepatitis in developing countries. interestingly, an increasing number of sporadic cases of hev infections are described in industrialized countries as zoonosis from domestic livestock. despite the increasing relevance of this pathogen in clinical virology, commercial antibody assays are mainly based on fragments of hev open reading frame (orf) 2 and orf3. the ... | 2012 | 22269698 |
| epidemiological and virological characteristics of symptomatic acute hepatitis e in greater cairo, egypt. | the aim of the study was to describe the characteristics of acute hepatitis e in greater cairo. patients with acute hepatitis e were identified through a surveillance of acute hepatitis using the following definition: recent (<3 weeks) onset of fever or jaundice, alanine aminotransferase at least three times the upper limit of normal (uln), negative markers for other causes of viral hepatitis and detectable hepatitis e virus (hev) rna. comparison of the liver tests between acute hepatitis e and ... | 2012 | 22264267 |
| hiv, hev and cirrhosis: evidence of a possible link from eastern spain. | the aim of the study was to assess the seroprevalence of hepatitis e virus (hev) infection in an hiv-infected population, as determined by hev immunoglobulin g (igg) antibodies (anti-hev). | 2012 | 22257075 |
| coronavirus infection of rat dorsal root ganglia: ultrastructural characterization of viral replication, transfer, and the early response of satellite cells. | swine hemagglutinating encephalomyelitis virus (hev) has been shown to have a capability to gain access to the cell bodies of sensory neurons after peripheral inoculation, resulting in ganglionic infection. it is not clearly understood how this virus is replicated within and released from the sensory neurons, and it remains to know how satellite cells response to the hev invasion. by ultrastructurally examining hev-infected rat dorsal root ganglia, we found that hev in the cell bodies of infecte ... | 2012 | 22248641 |
| immunohistochemistry for the diagnosis of hepatitis e virus infection. | hepatitis e virus (hev) is an emerging pathogen and the most common cause of acute viral hepatitis all over the world. we describe here an immunohistochemical method for the detection of hev antigens (porf2 and porf3) in formalin-fixed, paraffin-embedded liver tissues using monoclonal antibodies raised against two of the virus proteins (porf2 and porf3). we analysed their specificity and sensitivity in comparison with serology and nucleic acid detection in cases of acute liver failure (alf). we ... | 2011 | 22239516 |
| seroepidemiology and molecular characterization of hepatitis e virus in macaca mulatta from a village in yunnan, china, where infection with this virus is endemic. | hepatitis e virus (hev) infection is a significant public health concern and has been identified as a zoonotic infection. | 2011 | 22235219 |
| hepatitis e virus-specific t-cell response after transplantation. | 2012 | 22234861 | |
| ephrin-b2 and ephrin-b3 as functional henipavirus receptors. | members of the ephrin cell-surface protein family interact with the eph receptors, the largest family of receptor tyrosine kinases, mediating bi-directional signaling during tumorogenesis and various developmental events. surprisingly, ephrin-b2 and -b3 were recently identified as entry receptors for henipaviruses, emerging zoonotic paramyxoviruses responsible for repeated outbreaks in humans and animals in australia, southeast asia, india and bangladesh. nipah virus (niv) and hendra virus (hev) ... | 2011 | 22227101 |
| emerging and re-emerging swine viruses. | in the past two decades or so, a number of viruses have emerged in the global swine population. some, such as porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2), cause economically important diseases in pigs, whereas others such as porcine torque teno virus (ttv), now known as torque teno sus virus (ttsuv), porcine bocavirus (pbov) and related novel parvoviruses, porcine kobuvirus, porcine toroviruses (ptov) and porcine lymphotropic herpesviruses (pl ... | 2012 | 22225855 |
| hepatitis e virus infection in macaca mulatta. | 2011 | 22224088 | |
| occurrence of hepatitis e virus rna in plasma donations from sweden, germany and the united states. | 2012 | 22220775 | |
| hepatitis e virus in norway rats (rattus norvegicus) captured around a pig farm. | hepatitis e virus (hev) transmitted via the oral route through the consumption of contaminated water or uncooked or undercooked contaminated meat has been implicated in major outbreaks. rats may play a critical role in hev outbreaks, considering their negative effects on environmental hygiene and food sanitation. although the serological evidence of hev infection in wild rodents has been reported worldwide, the infectivity and propagation of hev in wild rats remain unknown. to investigate if rat ... | 2012 | 22217009 |
| hepatitis e: epidemiology and prevention. | hepatitis e is caused by the hepatitis e virus (hev), the major etiologic agent of enterically transmitted non-a hepatitis worldwide. hev is responsible for major outbreaks of acute hepatitis in developing countries, especially in many parts of africa and asia. the hev is a spherical, non-enveloped, single-stranded, positive sense rna virus that is approximately 32 nm to 34 nm in diameter and is the only member in the family hepeviridae and genus hepevirus. there are four distinct genotypes of h ... | 2011 | 22216368 |
| chronic hepatitis e in hematopoietic stem cell transplant patients in a low-endemic country? | cases of chronic hepatitis e have been described in patients after kidney and liver transplantation. in addition, hepatitis e virus (hev) reactivation was reported after hematopoietic stem cell transplantation (hsct). we here evaluated if hev infection might explain elevated liver enzymes in a well selected cohort of allogeneic hsct patients with biochemical evidence of hepatitis (n = 52). of note, none of the subjects tested positive for hev rna, including 2 hsct patients who had been infected ... | 2011 | 22212560 |
| optimisation of a triplex real time rt-pcr for detection of hepatitis e virus rna and validation on biological samples. | the aim of this study was to optimise a two-tube reverse transcription triplex quantitative real time pcr (qrt-pcr) combining amplification of two loci with an internal amplification control (iac) for detection and quantitation of hepatitis e virus (hev) rna and to validate its performance on a pool of biological samples. optimisation was performed on serially diluted "home-made" rna standards. the limit of detection was determined experimentally as 10copies/μl of the rna standard for both ampli ... | 2011 | 22207081 |
| Detection of negative sense RNA in packaged hepatitis E virions using improved strand specific RT-PCR method. | Current hepatitis E virus (HEV) negative sense RNA detection assays have drawback of false positivity. cDNA synthesis using tag-based primer and Superscript RT-III followed by exonuclease I treatment increased the specificity. Assay could detect as low as 10 copies of negative sense RNA and could be used in detecting low levels of HEV replication in cells. Virus particles in stool samples of hepatitis E patients showed encapsidation of negative sense RNA along with HEV genomic RNA. | 2011 | 22205803 |
| genotype 3 diversity and quantification of hepatitis e virus rna. | genotype 3 hepatitis e viruses (hev) are distributed across the world and are now considered to be an emerging public health concern in industrialized countries. at least 10 genotype 3 subtypes have been identified in humans and animals worldwide. it was recently reported that the sensitivities of hev rna assays differ greatly. we have assessed the influence of genotype 3 diversity on the performance of two hev rna assays: one targeting the orf3 gene and the other targeting the orf2 gene. we tes ... | 2011 | 22205792 |
| current status of hepatitis e virus infection in korea. | hepatitis e virus (hev) is an emerging pathogen associated with acute viral hepatitis, and hev is becoming increasingly recognized. approximately 2% of acute viral hepatitis is caused by hev, and 18 cases of hepatitis e have been reported in korea. of these cases, only two have involved a history of travel from india, which suggests that they were imported cases. the remaining reported cases include a sporadic case of acute hepatitis e with genotype 4 hev isolates and identification of the full ... | 2011 | 22195239 |
| suppression of alpha interferon signaling by hepatitis e virus. | the interferon (ifn) system is integral to the host response against viruses to which many viruses have developed strategies to overcome its antiviral effects. the effects of hepatitis e virus (hev), the causative agent of hepatitis e, on ifn signaling have not been investigated primarily because of the non-availability of an efficient in vitro culture system or small animal models of infection. herein, we report the generation of a549 cell lines persistently infected with genotype 3 hev, design ... | 2011 | 22183878 |
| New findings regarding the epidemic history and population dynamics of Japan-indigenous genotype 3 hepatitis E virus inferred by molecular evolution. | BACKGROUND: Since previous studies have investigated the population dynamics of Japan-indigenous genotype 3 hepatitis E virus (HEV) using virus sequences, more nucleotide sequences have been determined, and new techniques have been developed for such analysis. AIMS: To prevent future hepatitis E epidemic in Japan, this study aimed to elucidate the cause of past HEV expansion. METHODS: The epidemic history of Japan-indigenous genotype 3 HEV was determined using the coalescent analysis framework. ... | 2011 | 22181032 |
| seroprevalence study in forestry workers from eastern germany using novel genotype 3- and rat hepatitis e virus-specific immunoglobulin g elisas. | hepatitis e virus (hev) is the causative agent of an acute self-limiting hepatitis in humans. in industrialized countries, autochthonous cases are linked to zoonotic transmission from domestic pigs, wild boar and red deer. the main route of human infection presumably is consumption of contaminated meat. farmers, slaughterers and veterinarians are expected to be risk groups as they work close to potentially infected animals. in this study, we tested four escherichia coli-expressed segments of the ... | 2011 | 22179131 |
| hepatitis e in three immunocompromized children in southeastern france. | hepatitis e virus (hev) causes an emerging autochthonous disease in developed countries where links with a viral porcine reservoir have been evidenced. moreover, chronic hev infection and associated-cirrhosis have been described in severely immunocompromized patients. nonetheless, only few studies have focused on pediatric hev infections worldwide and only four autochthonous cases have been reported in children in developed countries. we describe here acute hepatitis e in three immunocompromized ... | 2011 | 22177988 |
| Hepatitis e virus in rats, los angeles, california, USA. | The role of rats in human hepatitis E virus (HEV) infections remains controversial. A genetically distinct HEV was recently isolated from rats in Germany, and its genome was sequenced. We have isolated a genetically similar HEV from urban rats in Los Angeles, California, USA, and characterized its ability to infect laboratory rats and nonhuman primates. Two strains of HEV were isolated from serum samples of 134 wild rats that had a seroprevalence of antibodies against HEV of ˜80%. Virus was tran ... | 2011 | 22172320 |
| hepatitis e virus antibodies in blood donors, france. | using a validated sensitive assay, we found hepatitis e virus (hev) igg in 52.5% of voluntary blood donors in southwestern france. this finding suggests hev is highly endemic to this region. the high hev prevalence may reflect local dietary practices, such as eating uncooked pork and game products. | 2011 | 22172156 |
| Experimental infection of horses with hendra virus/australia/horse/2008/redlands. | Hendra virus (HeV) is a highly pathogenic zoonotic paramyxovirus harbored by Australian flying foxes with sporadic spillovers directly to horses. Although the mode and critical control points of HeV spillover to horses from flying foxes, and the risk for transmission from infected horses to other horses and humans, are poorly understood, we successfully established systemic HeV disease in 3 horses exposed to Hendra virus/Australia/Horse/2008/Redlands by the oronasal route, a plausible route for ... | 2011 | 22172152 |
| Site occupancy and glycan compositional analysis of two soluble recombinant forms of the attachment glycoprotein of Hendra virus. | Hendra virus (HeV) continues to cause morbidity and mortality in both humans and horses with a number of sporadic outbreaks. HeV has two structural membrane glycoproteins that mediate infection of host cells: the attachment (G) and the fusion (F) glycoproteins that are essential for receptor binding and virion-host cell membrane fusion, respectively. N-linked glycosylation of viral envelope proteins are critical post-translation modifications that have been implicated in roles of structural inte ... | 2011 | 22171062 |
| seroprevalence evolution of selected pathogens in iberian wild boar. | a total of 1279 eurasian wild boar (sus scrofa) sera were collected from 2000 to 2011 in the iberian peninsula to reveal time changes in serum antibody prevalences against selected infectious agents (porcine circovirus type 2, pcv2; porcine reproductive and respiratory syndrome virus, prrsv; hepatitis e virus, hev; and erysipelothrix rhusiopathiae) and to identify putative individual or population factors driving such changes. overall seroprevalences were 48%, 26%, 2% and 15% for pcv2, hev, prrs ... | 2011 | 22168900 |
| Molecular epidemiology of hepatitis E virus infections in Shanghai, China. | ABSTRACT: BACKGROUND: Hepatitis E virus (HEV) causes acute or fulminant hepatitis in humans and is an important public health concern in many developing countries. China has a high incidence of HEV epidemics, with at least three genotypes (1, 3, and 4) and nine subtypes (1b, 1c, 3b, 4a, 4b, 4d, 4g, 4h and 4iso far identified. Since genotype 3 and the newly identified subtype 4i have been exclusively limited geographically to Shanghai and its neighboring provinces, the epidemiology of HEV infect ... | 2011 | 22168371 |
| adaptation of genotype 3 hepatitis e virus in eastern china and inverse correlation with genotype 4 hepatitis e virus. | we studied the distribution and development of hepatitis e virus (hev) genotypes 3 and 4 in pig farms located within the shanghai metropolitan area. a total of 1,487 swine fecal samples were collected from 39 pig farms during 2009-2010. the average incidence rates for genotype 3 and genotype 4 hev were 10.6 and 9.3%, respectively. the frequencies of genotype 3 and genotype 4 hev among the farms were inversely related (r = -0.7423), suggesting that the two genotypes competed within the environmen ... | 2011 | 22156659 |
| seroprevalence of hepatitis e among iranian renal transplant recipients. | renal transplant recipients are known to be susceptible to viral infections, with more severe clinical presentations compared to healthy persons. hepatitis e is generally a self-limited disease, which is caused by hepatitis e virus. recently, hepatitis e has become more important in organ transplant recipients, because of new findings regarding the potential for chronic infections in this patient group. | 2011 | 22140389 |
| Hepatitis e virus and renal transplantation. | 2011 | 22140382 | |
| mri findings in acute hendra virus meningoencephalitis. | aim: to describe serial changes in brain magnetic resonance imaging (mri) in acute human infection from two outbreaks of hendra virus (hev), relate these changes to disease prognosis, and compare hev encephalitis to reported cases of nipah virus encephalitis. materials and methods: the mri images of three human cases (two of which were fatal) of acute hev meningoencephalitis were reviewed. results: cortical selectivity early in the disease is evident in all three patients, while deep white matte ... | 2011 | 22133593 |
| The global burden of hepatitis E virus. | We estimated the global burden of hepatitis E virus (HEV) genotypes 1 and 2 in 2005. HEV is an emergent waterborne infection that causes source-originated epidemics of acute disease with a case fatality rate thought to vary by age and pregnancy status. To create our estimates, we modeled the annual disease burden of HEV genotypes 1 and 2 for 9 of 21 regions defined for the Global Burden of Diseases, Injuries, and Risk Factors Study (the GBD 2010 Study), which represent 71% of the world's populat ... | 2011 | 22121109 |
| Transfusion-transmitted hepatitis E: Is screening warranted? | Hepatitis E virus (HEV) is an emerging infectious threat to blood safety. In recent years, there have been a number of publications delineating this threat by providing evidence of the transmissibility of this virus through transfusions. The extent of transmission and its clinical relevance are issues under debate at present. HEV usually causes a self-limiting illness which subsides in a few weeks barring a few cases where fulminant hepatic failure occurs. The virus poses a risk of higher morbid ... | 2011 | 22120793 |
| chronic hepatitis e infection in children with liver transplantation. | objectivechronic hepatitis e virus (hev) infection has been described in immunosuppressed adult patients. a study was undertaken to establish the presence of hev infection in children after orthotopic liver transplantation (olt).methodschildren undergoing liver transplantation between 1992 and 2010 with available serum were classified into two groups: group 1 (control group, n=66) with normal serum aminotransferases and group 2 (n=14) with persistently increased serum aminotransferases and histo ... | 2011 | 22115826 |
| second generation of pseudotype-based serum neutralization assay for nipah virus antibodies: sensitive and high-throughput analysis utilizing secreted alkaline phosphatase. | nipah virus (niv), paramyxoviridae, henipavirus, is classified as a biosafety level (bsl) 4 pathogen, along with the closely related hendra virus (hev). a novel serum neutralization test was developed for measuring niv neutralizing antibodies under bsl2 conditions using a recombinant vesicular stomatitis virus (vsv) expressing secreted alkaline phosphatase (seap) and pseudotyped with niv f/g proteins (vsv-niv-seap). a unique characteristic of this novel assay is the ability to obtain neutralizat ... | 2012 | 22115786 |
| the epidemiology of hepatitis e virus infections in developed countries and among immunocompromised patients. | hepatitis e virus (hev) is an important cause of acute hepatitis in humans worldwide, both as epidemic and sporadic disease. since the virus was identified in 1983, epidemics have occurred regularly in many countries across south and southeast asia when seasonal floods have contaminated drinking water supplies and in africa during humanitarian crises among refugee populations without access to clean water. in addition, sporadic cases and small clusters of hev infections have been recognized thro ... | 2011 | 22114964 |
| A naturally-occurring human/hepatitis E recombinant virus predominates in serum but not in feces of a chronic hepatitis E patient and has a growth advantage in cell culture. | Summary. Hepatitis E virus is the etiological agent of acute hepatitis E, a self-limiting disease prevalent in developing countries. Molecular analysis of viral genomic RNA from a chronically-infected patient confirmed the recent discovery that chronic infection correlated with extensive diversification of the virus quasispecies: the hypervariable region of some virus genomes in this USA patient contained large continuous deletions and a minor portion of genomes in feces and serum had acquired a ... | 2011 | 22113007 |
| Hepatitis E virus genotype 3f in pigs in New Caledonia. | Hepatitis E virus (HEV) is highly prevalent in farm pigs worldwide and an increasing body of data from industrialised countries suggests that it is an agent of a porcine zoonosis. | 2011 | 22103949 |
| integrating overlapping structures and background information of words significantly improves biological sequence comparison. | word-based models have achieved promising results in sequence comparison. however, as the important statistical properties of words in biological sequence, how to use the overlapping structures and background information of the words to improve sequence comparison is still a problem. this paper proposed a new statistical method that integrates the overlapping structures and the background information of the words in biological sequences. to assess the effectiveness of this integration for sequen ... | 2011 | 22102867 |
| [experience in the diagnosis of hepatitis e in a tropical infectious diseases unit.] | the hepatitis e virus (hev) is endemic in some developing countries. it produces acute hepatitis in sporadic cases or epidemics. the main transmission route is faecal-oral by contaminated waters. in developed countries the cases described are more and more frequent, mainly due to population movement (travellers, immigrants, international adoptions), although there have also been increases in the local population. we currently have serological and molecular techniques for the diagnosis of this in ... | 2011 | 22100778 |
| Hepatitis E virus in rabbits, Virginia, USA. | We identified hepatitis E virus (HEV) in rabbits in Virginia, USA. HEV RNA was detected in 14 (16%) of 85 serum samples and 13 (15%) of 85 fecal samples. Antibodies against HEV were detected in 31 (36%) of 85 serum samples. Sequence analyses showed that HEV from rabbits is closely related to genotype 3. | 2011 | 22099094 |
| Close similarity between sequences of hepatitis E virus recovered from humans and swine, France, 2008-2009. | Frequent zoonotic transmission of hepatitis E virus (HEV) has been suspected, but data supporting the animal origin of autochthonous cases are still sparse. We assessed the genetic identity of HEV strains found in humans and swine during an 18-month period in France. HEV sequences identified in patients with autochthonous hepatitis E infection (n = 106) were compared with sequences amplified from swine livers collected in slaughterhouses (n = 43). Phylogenetic analysis showed the same proportion ... | 2011 | 22099089 |
| [virological characteristics of hepatitis e virus]. | 2011 | 22096983 | |
| Prevalence of specific antibody to hepatitis E virus in the general population of the community of Madrid, Spain. | Hepatitis E virus (HEV) is an infectious agent causing hepatitis among humans. Although hepatitis E has been reported from many European countries, its incidence in Europe is largely unknown, and the prevalence of the HEV infection is also unknown for most countries of the region. Antibody to HEV (anti-HEV) was tested on 2,305 serum samples from the general population of the Community of Madrid (Spain) collected in the year 2008 among people aged 2-60 years. Total anti-HEV was tested by enzyme-i ... | 2012 | 22095537 |
| hepatitis e virus: an underdiagnosed cause of chronic hepatitis in renal transplant recipients. | hepatitis e virus (hev) infection can evolve to chronic hepatitis in immunocompromised patients leading to rapidly progressive cirrhosis. proper diagnosis is therefore important, as reducing immunosuppressive therapy can allow clearance of the virus. we report a case of chronic hev infection in a renal transplant recipient that went undiagnosed for many years, discuss the therapeutic options, and review the current available literature. | 2011 | 22093456 |
| virus hazards from food, water and other contaminated environments. | numerous viruses of human or animal origin can spread in the environment and infect people via water and food, mostly through ingestion and occasionally through skin contact. these viruses are released into the environment by various routes including water run-offs and aerosols. furthermore, zoonotic viruses may infect humans exposed to contaminated surface waters. foodstuffs of animal origin can be contaminated, and their consumption may cause human infection if the viruses are not inactivated ... | 2011 | 22091646 |
| prevalence of hepatitis e virus and porcine caliciviruses in pig farms of guizhou province, china. | backgroud: hepatitis e virus (hev) and caliciviruses are enteric pathogens of humans and animals, and pigs have been considered an important reservoir of these viruses. | 2011 | 22087178 |
| fatal course of an autochthonous hepatitis e virus infection in a patient with leukemia in germany. | an acute infection with hepatitis e virus (hev) genotype 3 subtype c was diagnosed in a patient with chronic lymphatic b-cell leukemia 6 weeks after the infusion of donor lymphocytes. despite intensive care the patient died 39 days after admission due to pericardial effusion that was related to acute liver failure. we suggest that diagnostic procedures for detection of hev infection should be seriously considered for the immunocompromised patient with elevated liver enzymes in the absence of a t ... | 2011 | 22086667 |
| no reactivation of hepatitis e virus after kidney retransplantation. | 2011 | 22070620 | |
| Bacteriological quality of water and water borne diseases in Bangalore: a longitudinal study. | In developing countries diarrhea diseases take a big toll which can be prevented by adequate supply of safe drinking water. Thus a longitudinal study was taken up to determine the morbidity due to water borne diseases and bacteriological quality of water. 150 houses in two different areas, one supplied by bore well and other by tap water was selected by modified cluster sampling. Weekly morbidity details collected. Monthly water samples were assessed for bacteriological quality from main supply, ... | 2011 | 22066282 |
| Survey of enterovirus infections from hand, foot and mouth disease outbreak in china, 2009. | ABSTRACT: | 2011 | 22054534 |
| risk factors and immune response to hepatitis e viral infection among acute hepatitis patients in assiut, egypt. | hepatitis e virus (hev) infection is a common cause of acute viral hepatitis (avh) in egypt. we aimed to identify risk factors of hev among acute hepatitis cases, measure hev specific immune response to differentiate between symptomatic and asymptomatic infections. the study included symptomatic acute hepatitis (ah) patients (n = 235) and asymptomatic contacts (n = 200) to hev cases. they completed a lifestyle questionnaire, screened for common hepatotropic viruses. blood and serum samples were ... | 2010 | 22053611 |
| Pteropid bats are confirmed as the reservoir hosts of henipaviruses: a comprehensive experimental study of virus transmission. | Bats of the genus Pteropus have been identified as the reservoir hosts for the henipaviruses Hendra virus (HeV) and Nipah virus (NiV). The aim of these studies was to assess likely mechanisms for henipaviruses transmission from bats. In a series of experiments, Pteropus bats from Malaysia and Australia were inoculated with NiV and HeV, respectively, by natural routes of infection. Despite an intensive sampling strategy, no NiV was recovered from the Malaysian bats and HeV was reisolated from onl ... | 2011 | 22049055 |
| a549 and plc/prf/5 cells can support the efficient propagation of swine and wild boar hepatitis e virus (hev) strains: demonstration of hev infectivity of porcine liver sold as food. | recent evidence has indicated the cross-species transmission of hepatitis e virus (hev) from pigs and wild boars to humans, causing zoonosis, mostly via consumption of uncooked or undercooked animal meat/viscera. however, no efficient cell culture system for swine and boar hev strains has been established. we inoculated a549 cells with 12 swine and boar hev strains of liver, feces, or serum origin at an hev load of ≥2.0 × 10(4) copies per well and found that the hev progeny replicated as efficie ... | 2011 | 22048607 |
| [the etiological identification of an aseptic encephalitis outbreak (ten cases) in a hospital of xiamen city, 2011]. | to identify the etiology of an aseptic encephalitis outbreak (ten cases) in a hospital of xiamen city from 11 to 17 may, 2011. | 2011 | 22041567 |