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influence of 5 major salmonella pathogenicity islands on nk cell depletion in mice infected with salmonella enterica serovar enteritidis.in this study we were interested in the colonisation and early immune response of balb/c mice to infection with salmonella enteritidis and isogenic pathogenicity island free mutants.201020226037
mycobacterium bovis bcg-itis and cervical lymphadenitis due to salmonella enteritidis in a patient with complete interleukin-12/-23 receptor beta1 deficiency.mendelian susceptibility to mycobacterial disease (msmd) is a rare disorder with predisposition to severe, sometimes lethal, disease caused by otherwise poorly virulent, non-tuberculous environmental mycobacteria and poorly virulent salmonellae. in patients with msmd, mutations have been identified in five genes that encode for the proteins il-12/il-23p40, il-12/ il-23rbeta1, ifn-r1, ifn-gammar2 and stat1. these proteins play important roles in the type-1 cytokine pathway, which is crucial for h ...201020213287
salmonella enteritidis in chicken carcasses and giblets in southern chile.gastroenteritis caused by salmonella spp. is mainly related to the consumption of undercooked chicken meat or raw poultry products. the objective of this study was to assess the occurrence of salmonella spp. in chicken carcasses and giblets commercialized in southern chile (valdivia city).201020212342
pcr multiplex for detection of salmonella enteritidis, typhi and typhimurium and occurrence in poultry meat.the occurrence of foodborne diseases is increasing throughout the world. bacteria of the genus salmonella are responsible for food poisoning and, in some cases, may be fatal. the aim of this study was to adapt the multiplex pcr technique (mpcr) on the rapid and direct identification of the presence of salmonella sp. as well as serotypes enteritidis, typhi and typhimurium in poultry carcasses (n=127) and viscera (n=73). the implementation of the standard technique using positive controls was succ ...201020199820
antimicrobial resistance, class 1 integrons, and genomic island 1 in salmonella isolates from vietnam.the objective was to investigate the phenotypic and genotypic resistance and the horizontal transfer of resistance determinants from salmonella isolates from humans and animals in vietnam.201020195474
anti-infective mechanisms induced by a probiotic lactobacillus strain against salmonella enterica serovar typhimurium infection.the prevention of pathogen infections is one of the most extensively studied effects of probiotics. l. casei crl 431 is a probiotic bacterium and its effects on the gut immune cells have been extensively studied. the aim of the present study was to determine, using a mouse model, the preventive and therapeutic effect of l. casei crl 431 to achieve protection against salmonella enteritidis serovar typhimurium infection. in both previous and continuous (previous and post-infection) probiotic admin ...201020193971
flagellin expression enhances salmonella accumulation in tlr5-positive macrophages.recently, it has been reported that salmonella secrete flagellin in response to host produced lysophospholipids. however, this monomer of the bacterial flagella activates toll-like receptor 5 (tlr5) in the innate immune system. the objective of this study was to examine the role of flagellin expression during infection of species-specific macrophages (mphi) which either expressed or lacked tlr5. initially, tlr5-activity was confirmed in bovine mphi using salmonella typhimurium derived-flagellin. ...201020188752
salmonella meningitis and septicaemia in an non-immunocompromised adult, associated with a cluster of salmonella enteritidis pt 14b, ireland, november 2009.we report a fatal case of meningitis caused by salmonella enteritidis phage type 14b in a middle-aged man who had no history or findings to suggest he was immunocompromised. to our knowledge, this is the first reported case of salmonella meningitis in an adult in ireland, and the first case of meningitis in an adult caused by phage type 14b. this case was associated with a nationwide cluster of salmonellosis which is still under investigation at the time of writing.201020184853
evaluation of steam pasteurization in controlling salmonella serotype enteritidis on raw almond surfaces.to investigate the efficacy of steam pasteurization for reducing salmonella serotype enteritidis on raw almond surfaces.201020184671
succesful [successful] endovascular repair of an abdominal mycotic aneurysm. a case report.a 73-year-old man was admitted to the emergency department presenting with cramping pain in the left iliac fossa that had persisted for ten days, with diarrhoea and dysuria. a recent onset of high fever had urged him to the hospital. a routine blood sample showed a leukocytosis of 11.9 x 10(3)/mm(3) and an elevated crp of 16 mg/dl. haemocultures and a urine sample were taken. a colonoscopy and ultrasound showed no abnormalities. the urine culture contained salmonella enteritidis. on day 5 a ct s ...200920184071
acute tubulointerstitial nephritis complicated by salmonella enteritidis gastroenteritis.invasive non-typhoidal salmonellosis may occur in otherwise healthy children. we report an immunocompetent 3-year-old boy with salmonella enteritidis gastroenteritis complicated by acute tubulointerstitial nephritis who presented with fever, bloody diarrhea and gross hematuria. this case is the first report of non-typhoidal salmonellosis associated with biopsy-proven tubulointerstitial nephritis in a child.201020178727
spontaneous excision of the salmonella enterica serovar enteritidis-specific defective prophage-like element phise14.salmonella enterica serovar enteritidis has emerged as a major health problem worldwide in the last few decades. dna loci unique to s. enteritidis can provide markers for detection of this pathogen and may reveal pathogenic mechanisms restricted to this serovar. an in silico comparison of 16 salmonella genomic sequences revealed the presence of an approximately 12.5-kb genomic island (gei) specific to the sequenced s. enteritidis strain nctc13349. the gei is inserted at the 5' end of gene ydao ( ...201020172996
survival of salmonella enteritidis during salting and drying of horse mackerel (trachurus trachurus) fillets.survival of salmonella enteritidis inoculated (5-6 log cfu/g) on horse mackerel fillets was studied during salting and/or drying processes at 4 degrees c for up to 70days. sample groups were as follows: group a (salt/fish=80/100), group b (salt/fish=30/100), group c (salted as group a and dried after 15days), group d (salted as group b and dried after 15days), and group e (dried without salting). the salt content increased in all groups with a maximum level of 29.36%. final salt contents (%) wer ...201020172617
analysis of the correlation between the level of anti-salmonella antibodies in egg yolks and the presence of these microorganisms in egg contents following experimental infection of hens with salmonella enteritidis and after treatment with selected antibiotics.serological tests applied in poultry flocks can be a valuable tool in assessing health of hens. one obstacle in making this assessment is that results of serological tests in a given flock are not always correlated with results of bacteriological tests. the aim of this study was to determine dependencies between the level of antibodies in egg yolk and the contamination of egg contents (whites and yolks) with salmonella enteritidis bacilli. infected birds were also treated with selected antibioti ...200920169922
reconstitution of a functional toll-like receptor 5 binding site in campylobacter jejuni flagellin.bacterial flagellin is important for intestinal immune homeostasis. flagellins from most species activate toll-like receptor 5 (tlr5). the principal bacterial food-borne pathogen campylobacter jejuni escapes tlr5 recognition, probably due to an alternate flagellin subunit structure. we investigated the molecular basis of tlr5 evasion by aiming to reconstitute tlr5 stimulating activity in live c. jejuni. both native glycosylated c. jejuni flagellins (flaa and flab) and recombinant proteins purifi ...201020164175
antibacterial effects of vulgarone b from artemisia iwayomogi alone and in combination with oxacillin.the antibacterial activities of vulgarone b, a component of artemisia iwayomogi essential oil, were evaluated against some antibiotic-susceptible and -resistant human pathogens. moreover, the effects of combining antibiotics, such as oxacillin, with vulgarone b were determined in this study. significant inhibitory activities of artemisia oils against antibiotic-susceptible and -resistant bacteria were confirmed by broth microdilution methods. the effects of vulgarone b on bacterial morphology an ...201020162399
control of salmonella enteritidis and salmonella gallinarum in birds by using live vaccine candidate containing attenuated salmonella gallinarum mutant strain.the ideal live vaccine to control salmonella in commercial chicken flocks should engender protection against various strains. the purpose of the present study was to confirm the attenuation of a salmonella gallinarum (sg) mutant strain with deletion on genes cobs and cbia, that are involved in the biosynthesis of cobalamin. furthermore, evaluate its use as a live vaccine against salmonella. for the evaluation of the vaccine efficacy, two experiments were conducted separately. birds from a commer ...201020153354
[complications of infected aneurysm due to salmonella enteritidis: a case report].salmonelloses are becoming more frequented. to date, very few cases of aneurisms or vascular grafts infections have been described. we report the rare case of an extradigestive manifestation, due to salmonella enteritidis affecting a patient with behçet disease. the biological diagnosis is based on microorganism isolation from the site of infection but also from blood cultures. the initial treatment combined antibiotherapy and surgical procedure and replacement of the arteriovenous graft.201020146980
antibacterial activity of carvacrol and 2-nitro-1-propanol against single and mixed populations of foodborne pathogenic bacteria in corn flour dough.cereal doughs are an important part of human diet, but at the same time can act as vehicles for the transmission of human pathogenic bacteria. in the present study, four pathogenic or toxinogenic bacteria (escherichia coli o157:h7, salmonella enterica serovar enteritidis, bacillus cereus and staphylococcus aureus) were inoculated in a dough made from corn flour in combination with the single antimicrobial compounds carvacrol and 2-nitro-1-propanol (2npoh). survival of single and mixed population ...201020141946
a time-course study of gene responses of chicken granulosa cells to salmonella enteritidis infection.consumption of eggs contaminated with salmonella enteritidis (se) has been recognized as one of the important causes of human foodborne salmonellosis. chicken granulosa cells (cgcs) comprise the last tissue layer surrounding the yolk in preovulatory follicles and are a preferred site for se invasion. to understand the cgc response to se infection, we conducted an in vitro time-course study to identify cgc transcriptional changes using chicken whole genome microarrays. the expression of 135 (4h p ...201020138717
[pleural empyema due to salmonella enteritidis]. 201020138691
[etiological analysis of enteric infectious diseases during beijing olympic games].to understand pathogen patterns of enteric infectious diseases and its impact on this pattern due to aggregation of a great deal of foreign visitors during beijing olympic games.200920137562
antibiotic resistance in salmonella isolates from imported chicken carcasses in bhutan and from pig carcasses in vietnam.the antibiotic resistance in salmonella isolates from 400 imported chicken carcasses in bhutan and from 178 pig carcasses in vietnam were analyzed on a random basis against 14 antimicrobial agents. among the poultry samples tested, 13% were positive for salmonella. salmonella enteritidis dominated with a prevalence of 80.7%, and 40 of the 42 isolates harbored two or more resistance determinants. for the 178 pigs investigated, 49.4% of the swabs and 34.8% of the lymph nodes were salmonella positi ...201020132687
multiplex real-time polymerase chain reaction assay for simultaneous detection and quantification of salmonella species, listeria monocytogenes, and escherichia coli o157:h7 in ground pork samples.salmonella sp., listeria monocytogenes, and escherichia coli o157:h7 are foodborne pathogens capable of causing serious gastrointestinal illness. we previously described simultaneous detection of these pathogens by multiplex polymerase chain reaction (pcr) in 44 types of spiked food samples, including meat, produce, fish, and dairy products, targeting genes specific for each pathogen. based on the previous work, a multiplex real-time pcr assay using fluorescent probes was developed to detect and ...201020132032
estimation of salmonella prevalence in uk egg-laying holdings.as part of an eu-wide programme to reduce the prevalence of salmonella in commercial egg-laying holdings, the eu has set for the uk an annual target of 10% reduction in the prevalence of salmonella enteritidis and salmonella typhimurium in commercial egg-laying holdings. to assist in demonstrating such a reduction, it is very important to obtain an accurate as possible baseline prevalence for salmonella. the objective of this study was to provide a baseline estimate of the salmonella prevalence ...201020116871
microbiologic and clinical features of salmonella species isolated from bacteremic children in eastern democratic republic of congo.the morbidity of salmonella bloodstream infections is unacceptably high in africa. in 2000, the who global salmonella-surveillance (gss) program was founded to reduce the health burden of foodborne diseases. the incorporation, in 2002, of the democratic republic of congo (drc) in this program allowed the improvement of laboratory capacities. in this retrospective study, we describe the first signs of impact the gss program has had in drc in the management of bacteremia.201020104200
cembranoids from the octocoral sarcophyton ehrenbergi.chemical investigation of the octocoral sarcophyton ehrenbergi led to the isolation of six new cembranoids, (+)-12-carboxy-11z-sarcophytoxide (1), (+)-12-methoxycarbonyl-11z-sarcophine (3), ehrenberoxides a-c (4-6), and lobophynin c (2), along with two known compounds, (+)-sarcophytoxide (7) and (+)-sarcophine (8). the structures of these isolated metabolites were elucidated through extensive spectroscopic analyses, while the relative configuration of 1 was confirmed by x-ray diffraction analyse ...201020099902
inhibition of the early stage of salmonella enterica serovar enteritidis biofilm development on stainless steel by cell-free supernatant of a hafnia alvei culture.compounds present in hafnia alvei cell-free culture supernatant cumulatively negatively influence the early stage of biofilm development by salmonella enterica serovar enteritidis on stainless steel while they also reduce the overall metabolic activity of s. enteritidis planktonic cells. although acylhomoserine lactones (ahls) were detected among these compounds, the use of several synthetic ahls was not able to affect the initial stage of biofilm formation by this pathogen.201020097823
siderophore receptor iron is an important protective antigen against salmonella infection in chickens.in iron-limiting environments, salmonella enterica serovar enteritidis and salmonella enterica serovar typhimurium synthesize and secrete several types of siderophore to trap trivalent ferric ions; these bacteria then express siderophore receptors called iron-regulated outer membrane proteins (iromps). in this study, we experimentally reproduced iron-limiting environments using a divalent metal chelator. iron, one of the iromps, was purified by affinity chromatography with an anti-iron-mab-immob ...200920095157
effect of lactic acid bacteria probiotic culture treatment timing on salmonella enteritidis in neonatal broilers.in the present study, a series of experiments were conducted to evaluate the ability of a combination of 3 atcc lactobacilli (lab3) or a commercially available probiotic culture (prob) to reduce salmonella enterica serovar enteritidis (salmonella enteritidis) in broiler chicks. additionally, we varied the timing of prob administration in relationship to salmonella challenge and determined the influence on recovery of enteric salmonella. in experiments 1 to 3, chicks were randomly assigned to tre ...201020075275
a new antibacterial and antioxidant s07-2 compound produced by bacillus subtilis b38.an antibacterial compound, s07-2, was purified to homogeneity by hydrophobic interaction, anion exchange, c18 reverse-phase and hs peg hplc. the molecular mass of s07-2 was 905.6 da as determined by ms. the s07-2 compound was resistant to high temperatures (up to 100 degrees c) and could withstand a wide range of ph from 3 to 10. in addition, its antibacterial activity was preserved after treatment with proteases. biochemical characterization revealed its cyclic peptide structure. this compound ...201020070372
[a multi-pathogen selective enrichment broth for simultaneous growth of salmonella enteritidis, staphylococcus aureus, and listeria monocytogenes].a selective enrichment broth (ssl) was formulated to allow simultaneous growth of salmonella enteritidis, staphylococcus aureus, and listeria monocytogens.200920069888
effects of gut microflora on hepatic damage after acute liver injury in rats.liver injury often causes disruption of the gut microflora. the aim of this work was to evaluate the effects of microflora variations on acute liver injury.201020065761
a real-time pcr method for the detection of salmonella enterica from food using a target sequence identified by comparative genomic analysis.a 5'-nuclease real-time pcr assay using a minor groove binding probe was developed for the detection of salmonella enterica from artificially contaminated foods. s. enterica-specific sequences were identified by a comparative genomic approach. several species-specific target sequences were evaluated for specificity. a real-time pcr assay was developed targeting a nucleotide sequence within the putative type iii secretion atp synthase gene (ssan). an internal amplification control (iac) probe was ...201020060189
the antimicrobial effect of oregano essential oil, nisin and their combination against salmonella enteritidis in minced sheep meat during refrigerated storage.the antimicrobial effect of oregano essential oil (eo) at 0.6 or 0.9%, nisin at 500 or 1000 iu/g, and their combination against salmonella enteritidis was studied in minced sheep meat during storage at 4 degrees or 10 degrees c for 12 days. sensory evaluation showed that the addition of oregano eo at 0.6 or 0.9% in minced sheep meat was organoleptically acceptable, and attribute scores were higher for the eo at 0.6 than 0.9%. according to compositional analysis of the oregano eo, the phenols car ...201020060188
[optimization of the rep-pcr molecular classification method of salmonella and its application in drug-resistant strains].to optimize the reaction conditions of rep-pcr molecular classification method of salmonella; primarily apply it in drug-resistant strains, and supply data to a system of salmonella homology tracing.200920047237
[study on development of the principles for determination and evaluation of antimicrobial effectiveness of food preservatives].to establish the principles for determination and evaluation of the preservative properties demonstrates the effectiveness of a substance when used as a food preservative.200920047216
analysis of an outbreak of salmonella enteritidis by repetitive-sequence-based pcr and pulsed-field gel electrophoresis.the aim of this study was to investigate a large food-borne outbreak associated with eggs contaminated by salmonella enteritidis in a military unit using pulse field gel electrophoresis (pfge) and the repetitive-sequence-based pcr (rep-pcr) employing the diversilab system.201020045998
photodynamic antimicrobial activity of avian eggshell pigments.pigmentation in avian eggshells appears to be associated with shell strength, temperature regulation, and camouflage. the pigments found in eggshells are mainly porphyrins, which have been utilized therapeutically as photosensitizers. here, we examined the photoinactivation of gram-positive (staphylococcus aureus, bacillus cereus) and gram-negative bacteria (escherichia coli, salmonella enteritidis) by hen eggshells and their pigments. the results indicated that eggshells have a light-dependent ...201020036664
immune recovery effects of immunopotentiator from pantoea agglomerans 1 (ip-pa1) on low antibody productions in response to salmonella enteritidis vaccine and sheep red blood cells in dexamethasone-treated stressed chicken models.considering the usefulness of the immunopotentiator from pantoea agglomerans 1 (ip-pa1), which is a purified lipopolysaccharide (lps) derived from symbiotic gram-negative bacteria of food crops, in controlling immunosuppression in poultry husbandry, in this study, we examined its immune-recovery effects in dexamethasone-treated stressed chicken models. three-week-old chickens daily administered 10 microg/kg of dexamethasone for 35 days to induce stress showed more whole body weight loss; relativ ...201020035113
determination of the within and between flock prevalence and identification of risk factors for salmonella infections in laying hen flocks housed in conventional and alternative systems.salmonella outbreaks in humans are often linked with the consumption of contaminated eggs. therefore a profound knowledge of the actual prevalence of salmonella spp. in laying hens and the factors that influence the presence and persistence of salmonella on a farm is of utmost importance. the housing of laying hens in conventional battery cages will be forbidden in the european union (eu) from 2012 onwards. there is an urgent need to evaluate whether this move to alternative housing systems will ...201020031240
transfer of a phage t4 gene into enterobacteriaceae, determined at the single-cell level.the transfer range of phage genes was investigated at the single-cell level by using an in situ dna amplification technique. after absorption of phages, a phage t4 gene was maintained in the genomes of non-plaque-forming bacteria at frequencies of 10(-2) gene copies per cell. the gene transfer decreased the mutation frequencies in nonhost recipients.201020023087
virulence potential of five major pathogenicity islands (spi-1 to spi-5) of salmonella enterica serovar enteritidis for chickens.salmonella is a highly successful parasite of reptiles, birds and mammals. its ability to infect and colonise such a broad range of hosts coincided with the introduction of new genetic determinants, among them 5 major pathogenicity islands (spi1-5), into the salmonella genome. however, only limited information is available on how each of these pathogenicity islands influences the ability of salmonella to infect chickens. in this study, we therefore constructed salmonella enteritidis mutants with ...200920021686
potential zoonotic pathways of salmonella enteritidis in laying farms.salmonella enteritidis is a communicable zoonotic bacterium. the present investigation was done to evaluate the potential occurrence of salmonella enteritidis in laying hen farms and its contamination pathways. samples were collected from 10 laying hen farms located in the delta of egypt. cloacal swabs (n=300), eggshell swabs (n=400), and hand swabs from egg packagers (n=38) were collected. pools of ovary and oviduct were obtained from 150 hens; all samples were examined for the presence of salm ...201020020813
salmonella enteritidis necrotising fasciitis in a multiple myeloma patient receiving bortezomib. 201020020229
do infections trigger juvenile idiopathic arthritis?juvenile idiopathic arthritis (jia) is a disease that was prominent with increased inflammation response in immune system, appeared mostly with peripheral arthritis and endogenous and exogenous antigens play a role in the pathogenesis of disease. two major reasons were thinking to be considerably important. first of them is immunological predisposition and the second one is environmental factors. infections are considered to be the most important between environmental factors but also stress and ...201120012631
the effects of commercial cool water washing of shell eggs on haugh unit, vitelline membrane strength, aerobic microorganisms, and fungi.current egg washing practices use wash water temperatures averaging 49 degrees c and have been found to increase internal egg temperature by 6.7 to 7.8 degrees c. these high temperatures create a more optimal environment for bacterial growth, including salmonella enteritidis if it is present. salmonella enteritidis is the most common human pathogen associated with shell eggs and egg products. its growth is inhibited at temperatures of 7.2 degrees c and below. the objective of this study was to d ...201020008814
molecular and pathogenic characterization of salmonella enterica serovar bovismorbificans strains of animal, environmental, food, and human origin in hungary.in this study, we characterized 110 strains of salmonella enterica serovar bovismorbificans contaminating environment, animals, food of animal origin, and human, to assess their significance along the food chain in hungary. additionally, five strains from germany were tested for comparative purposes. characterization involved antibiotic susceptibility testing, class 1 integron detection by polymerase chain reaction, plasmid profiling, virulotyping (using virulence gene-specific polymerase chain ...201020001326
salmonella spp. infection in imported 1-day-old chicks, ducklings, and turkey poults: a public health risk.the occurrence of salmonella in 750 birds was assessed. the samples included the internal organs (caecal pouches, yolk sac, liver, and lung) of imported 1-day-old chicks (n = 150), grandparent chicks (n = 150), breeder chicks (n = 150), ducklings (n = 150), and turkey poults (n = 150), and paper-lined boxes (n = 250). salmonellae isolated from the internal organs and paper-lined box of 1-day-old chicks, ducklings, and poults were mostly evident from the paper-lined box followed by caecal samples ...201020001323
myocarditis mimicking an acute coronary syndrome: a case related to salmonella enteritis.infective myocarditis is most commonly due to a viral infection; occasionally it has been related to bacteria. gastrointestinal infections associated with myocarditis have only rarely been described in young people, and the pathogenesis is unclear. we report a case of myocarditis mimicking an acute coronary syndrome (acs) in a patient hospitalized for fever and diarrhoea. salmonella enteritidis was isolated from stool, and no other pathogens were found. the coronary angiography was normal, and t ...200919997519
modulating effect of microflora metabolites of the human and animals on lymphoid tissue culture. 200919994772
an outbreak of food poisoning caused by the bacillus enteritidis of gaertner in milk. 191419978157
subacute arthritis of shoulder-joint (due to an organism of the bacillus enteritidis type). 191219976355
electrostatic binding of substituted metal phthalocyanines to enterobacterial cells: its role in photodynamic inactivation.the effect of ionic substituents in zinc and aluminum phthalocyanine molecules and of membrane surface charge on the interaction of dyes with artificial membranes and enterobacterial cells, as well as on photosensitization efficiency was studied. it has been shown that increasing the number of positively charged substituents enhances the extent of phthalocyanine binding to escherichia coli cells. this, along with the high quantum yield of singlet oxygen generation, determines efficient photodyna ...200919961410
genotypic and phenotypic diversity of salmonella enteritidis isolated from chickens and humans in korea.salmonella enteritidis is the most common cause of salmonellosis in humans in south korea. it has been recognized that the principal source of human infection with s. enteritidis is chickens and their products such as meat and eggs. a total of 173 s. enteritidis isolates from humans (65 isolates) and chickens or their products (108 isolates) were analyzed by antibiotic susceptibility assay, phage typing, and pulsed-field gel electrophoresis (pfge). drug resistance was found to streptomycin (32.3 ...200919959892
serotype identification and antimicrobial resistance profiles of salmonella spp. isolated from chicken carcasses.in this study, 32 salmonella strains isolated from 400 chicken carcasses were serotyped, and antibiotic resistance profiles were detected against 12 selected antimicrobial agents using disc diffusion method. thirty-two isolates were identified as follows; 22 (68.7%) salmonella enteritidis, five (15.6%) salmonella virchow, three (9.3%) salmonella typhimurium and two (6.2%) salmonella hadar. in all salmonella isolates, antibiotic resistance were detected. out of 32 salmonella strains, 22 (68.75%) ...201019946796
mhc haplotype and susceptibility to experimental infections (salmonella enteritidis, pasteurella multocida or ascaridia galli) in a commercial and an indigenous chicken breed.in three independent experimental infection studies, the susceptibility and course of infection of three pathogens considered of importance in most poultry production systems, ascaridia galli, salmonella enteritidis and pasteurella multocida were compared in two chicken breeds, the indigenous vietnamese ri and the commercial luong phuong. furthermore, the association of the major histocompatibility complex (mhc) with disease-related parameters was evaluated, using alleles of the lei0258 microsat ...201019945754
a comparison of the efficacy of cleaning and disinfection methods in eliminating salmonella spp. from commercial egg laying houses.effective terminal cleaning and disinfection (c&d) is regarded as a necessary step for the elimination of salmonella spp. from laying houses. a total of 60 commercial laying houses that had housed laying flocks infected with salmonella enterica serovar enteritidis or salmonella enterica serovar typhimurium that were representative of all production systems (cage, barn, free-range) were intensively sampled immediately after c&d as well as in the follow-on flock. the procedures investigated were: ...200919937529
efficacy of several vaccination programmes in commercial layer and broiler breeder hens against experimental challenge with salmonella enterica serovar enteritidis.two experiments were performed to evaluate the protective effect of various vaccination combinations given at 5 and 9 weeks of age against experimental challenge with salmonella enterica serovar enteritidis (se) phage type 4 at 12 weeks of age. in experiment 1, groups of commercial layers were vaccinated by one of the following programmes: group 1, two doses of a se bacterin (layermune se); group 2, one dose of a live salmonella enterica serovar gallinarum vaccine (cevac sg9r) followed by one do ...200919937524
vectorial competence of larvae and adults of alphitobius diaperinus in the transmission of salmonella enteritidis in poultry.the ingestion of food products originating from poultry infected with salmonella spp. is one of the major causes of food poisoning in humans. the control of poultry salmonellosis is particularly difficult since birds are asymptomatic and numerous factors may expedite the maintenance of bacteria in poultry production facilities.201019929223
adjuvant effects for oral immunization provided by recombinant lactobacillus casei secreting biologically active murine interleukin-1{beta}.vaccine delivery systems using lactic acid bacteria are under development, but their efficiency is insufficient. autologous cytokines, such as interleukin-1beta (il-1beta), are potential adjuvants for mucosal vaccines and can be provided by recombinant lactic acid bacteria. the aim of this study was the construction and evaluation of recombinant lactobacillus casei producing il-1beta as an adjuvant delivery agent. the recombinant strain was constructed using an expression/secretion vector plasmi ...201019923575
genomic and phenotypic variation in epidemic-spanning salmonella enterica serovar enteritidis isolates.salmonella enterica serovar enteritidis (s. enteritidis) has caused major epidemics of gastrointestinal infection in many different countries. in this study we investigate genome divergence and pathogenic potential in s. enteritidis isolated before, during and after an epidemic in uruguay.200919922635
fitness costs and stability of a high-level ciprofloxacin resistance phenotype in salmonella enterica serotype enteritidis: reduced infectivity associated with decreased expression of salmonella pathogenicity island 1 genes.the fitness costs associated with high-level fluoroquinolone resistance were examined for phenotypically and genotypically characterized ciprofloxacin-resistant salmonella enterica serotype enteritidis mutants (104-cip and 5408-cip; mic, >32 microg/ml). the stability of the fluoroquinolone resistance phenotype in both mutants was investigated to assess whether clones with better fitness could emerge in the absence of antibiotic selective pressure. mutants 104-cip and 5408-cip displayed altered m ...201019917752
surface plasmon resonance (spr) as a rapid tool for serotyping of salmonella.an spr-based sandwich immunoassay for serotyping of salmonella is demonstrated. the salmonella are captured on an spr chip using polyclonal capture antibody. spr sensorgrams are obtained for the immunoreactions of the somatic (o) and flagellar (h) surface antigens, of the captured bacteria, to their respective antibodies. the sensorgram data are compiled to determine the antigenic formula in accordance with the kauffmann-white scheme. salmonella enteritidis was completely serotyped using this sp ...201019913402
salmonella serotype distribution in the dutch broiler supply chain.salmonella serotype distribution can give insight in contamination routes and persistence along a production chain. therefore, it is important to determine not only salmonella prevalence but also to specify the serotypes involved at the different stages of the supply chain. for this purpose, data from a national monitoring program in the netherlands were used to estimate the serotype distribution and to determine whether this distribution differs for the available sampling points in the broiler ...200919903970
effect of the housing system on shedding and colonization of gut and internal organs of laying hens with salmonella enteritidis.as a result of welfare considerations, conventional cage systems will be banned in the european union from 2012 onward. currently, there is limited information on the level of contamination with zoonotic pathogens related to the laying hen housing system. therefore, 2 studies were designed to investigate the effect of the housing system on colonization of layers with salmonella. in both studies, layers were housed in 3 different housing systems: a conventional cage system, a furnished cage, and ...200919903945
restaurant salmonella enteritidis outbreak associated with an asymptomatic infected food worker.salmonella is the most common bacterial cause of foodborne outbreaks in the united states; approximately half of salmonella outbreaks occur in restaurant settings. in february 2008, investigation of a cluster of salmonella enteritidis cases with indistinguishable pulsed-field gel electrophoresis (pfge) patterns revealed that five cases had eaten at the same restaurant. cases were identified through routine surveillance activities and by contacting meal companions of culture-confirmed cases. well ...200919903397
wild boars as an important reservoir for foodborne pathogens.one hundred fifty-three wild boars shot in the canton of geneva, switzerland, were studied for the occurrence of foodborne pathogens. tonsils and fecal samples of the animals were examined using real-time polymerase chain reaction, enzyme-linked fluorescent immunoassay, and cultural methods. the detection rate of salmonella spp., yersinia enterocolitica, yersinia pseudotuberculosis, stx-positive escherichia coli, and listeria monocytogenes was 12%, 35%, 20%, 9%, and 17%, respectively, when tonsi ...201019899962
cells in shearable and nonshearable regions of salmonella enterica serovar enteritidis biofilms are morphologically and physiologically distinct.cellular morphology, exopolymer chemistry, and protein expression of shearable and nonshearable fractions of salmonella enterica serovar enteritidis biofilms were examined. biofilms were grown at a laminar flow velocity of 0.07 cm.s-1 for ~120 h, resulting in biofilms with a thickness (mean +/- sd) of 43 +/- 24 microm. an empirically determined shear-inducing flow (1.33 cm.s-1) was then applied for 5 min, effectively reducing biofilm thickness by ~70% and leaving 13 +/- 6 microm of nonshearable ...200919898535
case report of pediatric gastroenteritis due to ctx-m-15 extended-spectrum beta-lactamase-producing salmonella enterica serotype enteritidis.a clinical isolate of salmonella enterica serotype enteritidis in korea was found to produce the extended-spectrum beta-lactamase ctx-m-15. the isolate was recovered in 2008 from the stool of a 3-yr-old boy with gastroenteritis. this isolate was found to be resistant to multiple drugs, including ampicillin, piperacillin, cefotaxime, ceftazidime, cefepime, and aztreonam. the resistance to cefotaxime was transferred by conjugation to recipient escherichia coli j53. the patient was eventually succe ...200919893356
synthesis, characterization, antibacterial activities and carbonic anhydrase enzyme inhibitor effects of new arylsulfonylhydrazone and their ni(ii), co(ii) complexes.ethane sulfonic acide hydrazide (esh: ch(3)ch(2)so(2)nhnh(2)) derivatives as 5-methylsalicyl-aldehydeethanesulfonylhydrazone (5msalesh), 5-methyl-2-hydroxyacetophenoneethane sulfonylhydrazone (5mafesh) and their ni(ii), co(ii) complexes have been synthesized for the first time. the structure of these compounds has been investigated by elemental analysis, ft-ir, (1)h nmr, (13)c nmr, lc/ms, uv-vis spectrophotometric method, magnetic susceptibility, thermal studies and conductivity measurements. th ...201019889572
nutrition of the host and natural resistance to infection : i. the effect of diet on the response of several genotypes of mus musculus to salmonella enteritidis infection.1. a diet of whole wheat and whole dried milk has been shown to promote a higher survival rate, among w-swiss mice subjected to s. enteritidis infection, than that promoted by a "synthetic" diet. 2. the demonstration of this ability of diet to condition natural resistance has been found to depend upon the genetic constitution of the mice employed. the demonstration has been possible in w-swiss mice, a strain only moderately inbred and retaining a degree of genetic variability. the demonstration ...194519871463
role of inborn resistance factors in mouse populations infected with bacillus enteritidis.1. under conditions in which mouse typhoid is allowed to spread naturally among herds of mice comprised of different proportions of individuals of innately high or low susceptibility: (a) 85 to 95 per cent of the innately susceptible succumb to mouse typhoid in contrast to less than 5 per cent of the innately resistant, regardless of whether either constitutes 25, 50, or 75 per cent of the population respectively. (b) the surviving population is therefore comprised largely of individuals known a ...193919870901
inherited and acquired factors in resistance to infection : ii. a comparison of mice inherently resistant or susceptible to bacillus enteritidis infection with respect to fertility, weight, and susceptibility to various routes and types of infection.1. mice with relatively great inherent resistance to certain bacterial infections were heavier but not more fertile than mice with relatively little inherent resistance. mice with relatively little inherent resistance were with one exception not abnormally low in weight. 2. b. enteritidis given intrastomachally to susceptibles appeared in the blood stream more promptly, in larger numbers, and in a greater percentage of cases, and was present in feces in larger numbers, for a longer period, and i ...193319870163
microbic virulence and host susceptibility in paratyphoid-enteritidis infection of white mice : ix. the relationship of dosage to mortality rate, survival time, and cage population.epidemics of mouse typhoid set up among the rockefeller institute strain of mice were studied over a period of 6 months. during this time the relationship of cage number of mouse typhoid bacilli to mortality, total population, and survival time was determined. a single virulence titration of the epidemic strain was made, and at the end of the experiment all survivors were examined for evidence of infection. the following conclusions may be drawn for the data here presented. 1. the available dosa ...192619869111
blood sugar studies : i. rapid alterations in the blood sugar level of rabbits as result of intravenous injections of killed bacteria of various types.a rapid rise in the blood sugar level of rabbits was produced by intravenous injections of killed bacillus proteus, bacillus coli, and bacillus paratyphosus b, which returned to nearly the previous level in a few hours time. a less pronounced rise in blood sugar was produced by killed bacillus paratyphosus a and bacillus enteritidis.192519869033
experimental epidemiology : ii. effect of the addition of healthy mice to a population suffering from mouse typhoid.1. a kind of mouse village was set up into which was introduced a small number of mice fed on a culture of so called mouse typhoid (bacillus pestis cavice of the bacillus paratyphosus b group) bacillus. the spread of the infection so induced to the cages, or "homes," of the other mice was left to accident through the attendant who fed the animals and cleaned the cages. that this means was likely to be sufficient was deduced from the epidemic reported by lynch. a spot map was kept throughout the ...192219868657
a group of paratyphoid bacilli from animals closely resembling those found in man.1. in addition to the paratyphoid bacilli already named there exists a group which occurs in a variety of animals and which culturally is the same as bacillus schottmülleri. as a rule this group can be separated from the latter by the type of clumps formed when bouillon cultures are used as antigens, while other antigens and complement fixation tests have failed to differentiate it. agglutination absorption tests sharply separate the animal from the human paratyphoids. 2. no differences have bee ...192019868428
a study of paratyphoid bacilli isolated from cases of hog-cholera.during the course of some experimental work on hog-cholera, paratyphoid bacilli were isolated from 16 per cent of the pigs. culturally these organisms are the same as paratyphoid 18 isolated from man, while they show several differences from hog-cholera bacilli. in their slight pathogenic effect on rabbits they also differ from the hog-cholera bacillus. in their agglutination in sera produced by the injection of living cultures, one of the cultures, isolated from a chronic case, corresponds to b ...191819868293
some theoretical considerations upon the nature oe agglutinins, together with further observations upon bacillus typhi abdominalis, bacillus enteritidis, bacillus coli communis, bacillus lactis aerogenes, and some other bacilli of allied character. 190119866950
characterization of salmonella enteritidis isolated from foods and patients in northern morocco.this study aimed to investigate salmonella enteritidis strains isolated from human and food sources in the north of morocco by means of phenotypic and genotypic methods.200919858571
infected abdominal aortic aneurysm caused by nontyphoid salmonella in an immunocompromised patient with rheumatoid arthritis.nontyphoid salmonella strains are important pathogens commonly found worldwide, typically causing gastrointestinal illness. here, we report a case of a 66-yearold man with an abdominal aortic infected (or so-called mycotic) aneurysm caused by salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis). he had multiple risk factors for atherosclerosis: age over 60, a long history of smoking, an 8-year history of diabetes mellitus, and a 10-year history of rheumatoid arthritis treated ...200919856069
salmonella colonisation of laying hens following vaccination with killed and live attenuated commercial salmonella vaccines.the aim of this study was to determine the efficacy of a killed salmonella vaccine and three live vaccines in preventing caecal colonisation of hy-line brown pullets by salmonella enteritidis pt 4. the lowest number of salmonella-positive birds following the largest challenge (10(8) cfu) was recorded for live vaccine 1. however, birds treated with the killed vaccine had a significantly lower number of salmonellae in their caeca compared with both the control group and the other vaccine groups (p ...200919855111
phage-type specific markers identified by diversity arrays technology (dart) analysis of salmonella enterica ssp. enterica serovars enteritidis and typhimurium.diversity arrays technology (dart) was applied to differentiate between s. enterica serovar enteritidis and typhimurium strains, respectively. ten and eleven, mainly phage and plasmid-related markers were identified for serovars enteritidis and typhimurium. in combination, these markers can be used for subtyping among and within phage types.201019852988
induction of cxc chemokine messenger-rna expression in chicken oviduct epithelial cells by salmonella enterica serovar enteritidis via the type three secretion system-1.the messenger-rna (mrna) expression of selected cytokines and chemokines in primary chicken oviduct epithelial cells (coec) was determined following in vitro infections with wild-type or type three secretion system (t3ss)-mutant salmonella enterica serovar enteritidis (se) strains. all se strains examined in this study elicited the expression of proinflammatory immune mediators including inducible nitric oxide synthase (inos), cxcli1 (k60), cxcli2 (il-8), ccli3 (k203), and ccli4 (mip-1beta). se ...200919848079
identification of salmonella enterica subspecies i, salmonella enterica serovars typhimurium, enteritidis and typhi using multiplex pcr.this study was designed to develop a multiplex pcr method with five specific primer pairs for the detection of salmonella spp., salmonella subspecies i, salmonella enterica serovars typhimurium, typhi and enteritidis. a multiplex pcr was constructed with five primer pairs for the detection of salmonella and pathogenic salmonella serovars, including a specific primer pair for salmonella typhi, based on the sequence comparison between genomic dna sequences of 12 salmonella strains. each primer pai ...200919843307
vaccination of chickens with recombinant salmonella expressing m2e and cd154 epitopes increases protection and decreases viral shedding after low pathogenic avian influenza challenge.avian influenza (ai) is a significant public health concern and serious economic threat to the commercial poultry industry worldwide. previous research demonstrates that antibodies against m2e confer protection against influenza challenge. using the red recombinase system in combination with overlapping extension pcr, we recently developed several novel attenuated salmonella enteritidis strains that express a protective m2e epitope in combination with a potential immune-enhancing cd154 peptide s ...200919834072
a rapid screen of broth enrichments for salmonella enterica serovars enteritidis, hadar, heidelberg, and typhimurium by using an allelotyping multiplex pcr that targets o- and h-antigen alleles.salmonella continues to cause significant foodborne outbreaks, best illustrated with recent outbreaks associated with peanut butter, raw tomatoes, and serrano peppers. to ascertain the likely source of the outbreak, bacterial typing is essential to this process. while pcr has become an important detection tool for pathogens in foods, pcr can also identify strain differences by targeting gene(s) or sequences exhibiting polymorphisms or variability in its distribution within the bacterial populati ...200919833046
brain abscess and epidural empyema caused by salmonella enteritidis in a child: successful treatment with ciprofloxacin: a case report.focal intracranial infections caused by salmonella are rare, especially those produced by s. enteritidis. we describe the case of a 26-month-old girl who underwent surgery for a frontoparietal ependymoma and presented with epidural empyema and a brain abscess due to s. enteritidis following an episode of gastroenteritis. the child was successfully treated by surgical drainage along with 9 weeks of antibiotic therapy including ciprofloxacin.200919829916
static magnetic field exposure fails to affect the viability of different bacteria strains.the viability of the microbes saccharomyces cerevisiae, bacillus circulans, escherichia coli, micrococcus luteus, pseudomonas fluorescens, salmonella enteritidis, serratia marcescens, and staphylococcus aureus was tested under static magnetic field exposure up to 24 h in either a homogeneous (159.2 +/- 13.4 mt) or three types of inhomogeneous static magnetic fields: (i) peak-to-peak magnetic flux density 476.7 +/- 0.1 mt with a lateral magnetic flux density gradient of 47.7 t/m, (ii) 12.0 +/- 0. ...201019821495
functional and molecular characterization of pse34 encoding a type iv secretion system in salmonella enterica serotype enteritidis phage type 34.salmonella enterica serotype enteritidis infection remains a serious public health threat to humans. salmonella enteritidis phage type 4 (pt4) is a clone that has already caused a global pandemic for years. to investigate why pt34 becomes a subdominantly emerging phage type, molecular characterizations, including serotyping, pulsed-field gel electrophoresis (pfge), phage typing, and plasmid profiling, were carried out on pt34. the results indicated that relative to pt4, pt34 contained an additio ...200919817860
emergence of extended-spectrum beta-lactamases and ampc-type beta-lactamases in human salmonella isolated in spain from 2001 to 2005.to study the resistance to third-generation cephalosporins in salmonella strains isolated from humans in a 5 year period in spain, and to identify the responsible genes and their dissemination.200919815633
[food poisonings are increasing. dangerous grilled meat (interview by dr. judith neumaier)]. 200919813530
identification of salmonella pullorum genomic sequences using suppression subtractive hybridization.pullorum disease affecting poultry is caused by salmonella enterica serovar pullorum and results in severe economic loss every year, especially in countries with a developing poultry industry. the pathogenesis of s. pullorum is not yet well defined, as the specific virulence factors still need to be identified. thus, to isolate specific dna fragments belonging to s. pullorum, this study used suppression subtractive hybridization. as such, the genome of the s. pullorum c79-13 strain was subtracte ...200919809245
acute pyelonephritis with renal abscesses and acute renal failure after salmonella infection.urinary tract infections, renal abscess formation and acute renal failure (arf) after salmonella infection are rarely reported in children. we present a previously healthy teenager who developed arf with renal abscess formation after salmonella infection, in whom we believe that acute salmonella pyelonephritis was the main causative factor for arf and not dehydration, shock or rhabdomyolysis, which have already been described in the literature. with prolonged antibiotic treatment and adequate hy ...201019804467
analysis of the variability in the number of viable bacteria after mild heat treatment of food.variability in the numbers of bacteria remaining in saline solution and whole milk following mild heat treatment has been studied with listeria innocua, enterococcus faecalis, salmonella enterica serovar enteritidis, and pseudomonas fluorescens. as expected, the most heat-resistant bacterium was e. faecalis, while p. fluorescens was the least heat resistant, and all bacteria showed greater thermal resistance in whole milk than in saline solution. despite the differences in the inactivation kinet ...200919801476
edible apple film wraps containing plant antimicrobials inactivate foodborne pathogens on meat and poultry products.apple-based edible films containing plant antimicrobials were evaluated for their activity against pathogenic bacteria on meat and poultry products. salmonella enterica or e. coli o157:h7 (10(7) cfu/g) cultures were surface inoculated on chicken breasts and listeria monocytogenes (10(6) cfu/g) on ham. the inoculated products were then wrapped with edible films containing 3 concentrations (0.5%, 1.5%, and 3%) of cinnamaldehyde or carvacrol. following incubation at either 23 or 4 degrees c for 72 ...200919799671
[salmonellosis in poland in 2007].the total number of 11,934 salmonellosis cases were reported in 2007 (13,362 in 2006), the incidence rate was 30.7 per 100,000 population. in total 251 outbreaks were reported involving 2 083 cases. the most common type of outbreaks were household outbreak. over seventy percent of patients were hospitalized. however, in outbreaks the proportion of hospitalized persons was significantly lower--34%. as in previous years the seasonal peak was observed in july and august. salmonella enteritidis was ...200919799251
overseas outbreaks of infectious intestinal disease identified in scotland, 2003 to 2007.this article presents information on all potential outbreaks of infectious intestinal disease (iid) identified by health protection scotland (hps) involving residents of scotland. unlike other systems, this alert system covers potential outbreaks of iid rather than apparently sporadic cases and covers all gastrointestinal pathogens, all visited countries, and all groups of travelers.200919796102
salmonella enteritidis bacteriophage candidates for phage therapy of poultry.salmonella is a worldwide foodborne pathogen causing acute enteric infections in humans. in the recent years, the use of bacteriophages has been suggested as a possible tool to combat this zoonotic pathogen in poultry farms. this work aims to isolate and perform comparative studies of a group of phages active against a collection of specific salmonella enteritidis strains from portugal and england. also, suitable phage candidates for therapy of poultry will be selected.201019796092
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