Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| centrifugal loop-mediated isothermal amplification microdevice for rapid, multiplex and colorimetric foodborne pathogen detection. | we present a centrifugal microfluidic device which enables multiplex foodborne pathogen identification by loop-mediated isothermal amplification (lamp) and colorimetric detection using eriochrome black t (ebt). five identical structures were designed in the centrifugal microfluidic system to perform the genetic analysis of 25 pathogen samples in a high-throughput manner. the sequential loading and aliquoting of the lamp cocktail, the primer mixtures, and the dna sample solutions were accomplishe ... | 2016 | 26322592 |
| genomic variability of serial human isolates of salmonella enterica serovar typhimurium associated with prolonged carriage. | salmonella enterica serovar typhimurium is an important foodborne human pathogen that often causes self-limiting but severe gastroenteritis. prolonged excretion of s. typhimurium after the infection can lead to secondary transmissions. however, little is known about within-host genomic variation in bacteria associated with asymptomatic shedding. genomes of 35 longitudinal isolates of s. typhimurium recovered from 11 patients (children and adults) with culture-confirmed gastroenteritis were seque ... | 2015 | 26311853 |
| [structure and function of the bacterial flagellar type iii protein export system in salmonella ]. | the bacterial flagellum is a filamentous organelle that propels the bacterial cell body in liquid media. for construction of the bacterial flagellum beyond the cytoplasmic membrane, flagellar component proteins are transported by its specific protein export apparatus from the cytoplasm to the distal end of the growing flagellar structure. the flagellar export apparatus consists of a transmembrane export gate complex and a cytoplasmic atpase ring complex. flagellar substrate-specific chaperones b ... | 2015 | 26310179 |
| genotypic diversity, pathogenic potential and the resistance profile of salmonella typhimurium strains isolated from humans and food from 1983 to 2013 in brazil. | salmonella enterica subsp. enterica serovar typhimurium is one of the leading serovars that causes salmonellosis worldwide. however, few studies have molecularly characterized s. typhimurium strains in brazil. in this study, we genotyped 92 s. typhimurium strains isolated from humans (43) and food (49) between 1983 and 2013 in brazil using pfge, multiple-locus variable number of tandem repeats analysis (mlva) and enterobacterial repetitive intergenic consensus pcr (eric-pcr). moreover, we assess ... | 2015 | 26307078 |
| structure-based design of inhibitors of the crucial cysteine biosynthetic pathway enzyme o-acetyl serine sulfhydrylase. | the cysteine biosynthetic pathway is of fundamental importance for the growth, survival, and pathogenicity of the many pathogens. this pathway is present in many species but is absent in mammals. the ability of pathogens to counteract the oxidative defences of a host is critical for the survival of these pathogens during their long latent phases, especially in anaerobic pathogens such as entamoeba histolytica, leishmania donovani, trichomonas vaginalis, and salmonella typhimurium. all of these o ... | 2016 | 26303427 |
| assessment by ames test and comet assay of toxicity potential of polymer used to develop field-capable rapid-detection device to analyze environmental samples. | there is need for devices that decrease detection time of food-borne pathogens from days to real-time. in this study, a rapid-detection device is being developed and assessed for potential cytotoxicity. the device is comprised of melt-spun polypropylene coupons coated via oxidative chemical vapor deposition (ocvd) with 3,4-ethylenedioxythiophene (edot), for conductivity and 3-thiopheneethanol (3te), allowing antibody attachment. the ames test and comet assay have been used in this study to exami ... | 2017 | 26301164 |
| construction of an oral vaccine for transmissible gastroenteritis virus based on the tgev n gene expressed in an attenuated salmonella typhimurium vector. | this research aimed to develop an oral vaccine for transmissible gastroenteritis virus (tgev) based on the tgev n gene expressed in an attenuated salmonella typhimurium vector and aimed to evaluate the vaccine's immune response in piglets. recombinant plasmid pvax-n was transformed into competent cells of attenuated s. typhimurium sl7207 via electroporation. after it was identified via rt-pcr and double digestion, the screened recombinant bacteria presenting pvax-n were named sl7207 (pvax-n). to ... | 2016 | 26297958 |
| draft genome sequences of 19 salmonella enterica serovar typhimurium [4,5:i:-] strains resistant to nalidixic acid from a long-term outbreak in italy. | here, we present the draft genome sequences of 19 salmonella enterica serovar typhimurium monophasic variant [4,5:i:-] strains involved in a long-term salmonellosis outbreak that occurred in central italy in 2013 to 2014. | 2015 | 26294627 |
| genetic control of bacterial biofilms. | nearly all bacterial species, including pathogens, have the ability to form biofilms. biofilms are defined as structured ecosystems in which microbes are attached to surfaces and embedded in a matrix composed of polysaccharides, edna, and proteins, and their development is a multistep process. bacterial biofilms constitute a large medical problem due to their extremely high resistance to various types of therapeutics, including conventional antibiotics. several environmental and genetic signals ... | 2016 | 26294280 |
| motility modulation by the small non-coding rna sroc in salmonella typhimurium. | bacterial regulatory networks of gene expression include the interaction of diverse types of molecules such as the small non-coding rnas (srnas) and their cognate messenger rnas (mrnas). in this study, we demonstrated that the salmonella typhimurium srna sroc is significantly expressed between the late-exponential and stationary phase of growth in an rpos-dependent manner. the expression of flagellar genes predicted as targets of this srna was quantitatively analyzed in both a δsroc mutant and a ... | 2015 | 26293911 |
| protection of neonatal broiler chickens following in ovo delivery of oligodeoxynucleotides containing cpg motifs (cpg-odn) formulated with carbon nanotubes or liposomes. | unformulated oligodeoxynucleotides (odn) containing cpg motifs (cpg-odn) have been shown to stimulate the innate immune system against a variety of bacterial, viral, and protozoan infections in a variety of vertebrate species. we have previously shown that in ovo delivery of unformulated cpg-odn was able to significantly protect neonatal broiler chickens against escherichia coli or salmonella typhimurium infections. the objectives of this study were to examine the safety and immunoprotective eff ... | 2015 | 26292531 |
| biofilm formation and genetic diversity of salmonella isolates recovered from clinical, food, poultry and environmental sources. | in the present study, salmonella isolates (n=40) recovered from clinical, food, poultry and environmental sources were characterized for serotype identification, genetic diversity and biofilm formation capability. serotype identification using multiplex pcr assay revealed six isolates to be salmonella typhimurium, 14 as salmonella enteritidis, 11 as salmonella typhi, and the remaining nine isolates unidentified were considered as other salmonella spp. most of the salmonella isolates (85%) produc ... | 2015 | 26292170 |
| salmonella induces pd-l1 expression in b cells. | salmonella persists for a long time in b cells; however, the mechanism(s) through which infected b cells avoid effector cd8 t cell responses has not been characterized. in this study, we show that salmonella infects and survives within all b1 and b2 cell subpopulations. b cells are infected with a salmonella typhimurium strain expressing an ovalbumin (ova) peptide (siinfekl) to evaluate whether b cells process and present salmonella antigens in the context of mhc-i molecules. our data showed tha ... | 2015 | 26292028 |
| antioxidant, mutagenic, and antimutagenic activities of tragopogon longirostis var. longirostis, an edible wild plant in turkey. | the ethanolic extract of tragopogon longirostis var. longirostis, a wild edible plant in anatolia was isolated, and its antioxidant, mutagenic, and antimutagenic properties were investigated. | 2017 | 26288475 |
| genetically modified "obligate" anaerobic salmonella typhimurium as a therapeutic strategy for neuroblastoma. | neuroblastoma currently has poor prognosis, therefore we proposed a new strategy by targeting neuroblastoma with genetically engineered anaerobic salmonella (sal-yb1). | 2015 | 26286454 |
| an avian influenza a (h7n9) virus vaccine candidate based on the fusion protein of hemagglutinin globular head and salmonella typhimurium flagellin. | a novel influenza virus, subtype h7n9, circulated through china in 2013-2014. its higher rates of human infection in a wide range of locations within china and the associated increased likelihood of human-to-human transmission have caused global concern. recombinant subunit vaccines provide safe and targeted protection against viral infections. however, the protective efficacy of recombinant subunit vaccines tends to be less potent than vaccines made from whole viruses. studies have shown that b ... | 2015 | 26286143 |
| vasculature disruption enhances bacterial targeting of autochthonous tumors. | attenuated salmonella enterica serovar typhimurium (s. typhimurium) has been developed as a vector to deliver therapeutic agents to tumors. the potential of s. typhimurium in cancer therapy is largely due to its reported propensity to accumulate at greater than 1,000-fold higher concentrations in tumors relative to healthy tissues. in this study, we compared bacterial colonization of tumors in a subcutaneous transplantation model with a more clinically relevant autochthonous tumor model. followi ... | 2015 | 26284135 |
| hha has a defined regulatory role that is not dependent upon h-ns or stpa. | the hha family of proteins is involved in the regulation of gene expression in enterobacteria by forming complexes with h-ns-like proteins. whereas several amino acid residues of both proteins participate in the interaction, some of them play a key role. residue d48 of hha protein is essential for the interaction with h-ns, thus the d48n substitution in hha protein abrogates h-ns/hha interaction. despite being a paralog of h-ns protein, stpa interacts with hhad48n with higher affinity than with ... | 2015 | 26284052 |
| mdsabc-mediated pathway for pathogenicity in salmonella enterica serovar typhimurium. | mdsabc is a salmonella-specific tripartite efflux pump that has been implicated in the virulence of salmonella enterica serovar typhimurium; however, little is known about the virulence factors associated with this pump. we observed mdsabc expression-dependent alterations in the degree of resistance to extracellular oxidative stress and macrophage-mediated killing. thin-layer chromatography and tandem mass spectrometry analyses revealed that overexpression of mdsabc led to increased secretion of ... | 2015 | 26283336 |
| toxic potentials of ten herbs commonly used for aphrodisiac effect in turkey. | sexual dysfunction is a serious problem worldwide. in turkey, herbal products are used by some people suffering from sexual dysfunction. despite their therapeutic advantages, some constituents of herbs are potentially toxic and pose health risks because they can be bought from the market without a prescription. therefore, we aimed to determine the safety of herbs possessing aphrodisiac effects, chosen on the basis of their frequency of medicinal use and commercial importance in turkey. | 2015 | 26281312 |
| antibacterial effects and action modes of asiatic acid. | in this study, the antibacterial effects and action modes of asiatic acid against the foodborne bacterial pathogens escherichia coli o157:h7, salmonella typhimurium dt104, pseudomonas aeruginosa, listeria monocytogenes, staphylococcus aureus, enterococcus faecalis, and bacillus cereus were examined. minimal inhibitory concentrations (mics) of asiatic acid against these bacteria were in the range of 20-40 μg/ml. minimum bactericidal concentrations of asiatic acid were in the range of 32-52 μg/ml. ... | 2015 | 26280399 |
| non-saccharomyces yeasts protect against epithelial cell barrier disruption induced by salmonella enterica subsp. enterica serovar typhimurium. | the human gastrointestinal epithelium makes up the largest barrier separating the body from the external environment. whereas invasive pathogens cause epithelial barrier disruption, probiotic micro-organisms modulate tight junction regulation and improve epithelial barrier function. in addition, probiotic strains may be able to reduce epithelial barrier disruption caused by pathogenic species. the aim of this study was to explore non-saccharomyces yeast modulation of epithelial cell barrier func ... | 2015 | 26280244 |
| prazosin-conjugated matrices based on biodegradable polymers and α-amino acids--synthesis, characterization, and in vitro release study. | novel and promising macromolecular conjugates of the α1-adrenergic blocker prazosin were directly synthesized by covalent incorporation of the drug to matrices composed of biodegradable polymers and α-amino acids for the development of a polymeric implantable drug delivery carrier. the cyto- and genotoxicity of the synthesized matrices were evaluated using a bacterial luminescence test, protozoan assay, and salmonella typhimurium ta1535. a new urethane bond was formed between the hydroxyl end-gr ... | 2015 | 26274943 |
| quantification of cytosolic vs. vacuolar salmonella in primary macrophages by differential permeabilization. | intracellular bacterial pathogens can replicate in the cytosol or in specialized pathogen-containing vacuoles (pcvs). to reach the cytosol, bacteria like shigella flexneri and francisella novicida need to induce the rupture of the phagosome. in contrast, salmonella typhimurium replicates in a vacuolar compartment, known as salmonella-containing vacuole (scv). however certain mutants of salmonella fail to maintain scv integrity and are thus released into the cytosol. the percentage of cytosolic v ... | 2015 | 26274778 |
| effect of rosemary essential oil and modified-atmosphere packaging (map) on meat quality and survival of pathogens in poultry fillets. | the effect of rosemary (rosmarinus officinalis l.) essential oil (reo) and modified-atmosphere packaging (map) on the survival of certain pathogens (salmonella typhimurium and listeria monocytogenes) in poultry fillets and on their meat quality during 7 days of refrigerated storage were investigated. because reo at 0.05% and 0.1% had weak antibacterial activity and reo at 0.3%, 0.5% and 1.0% imparted unacceptable organoleptic properties, only reo at 0.2% was used to treat the poultry meat. the r ... | 2015 | 26273279 |
| genotoxicity evaluation of oryeong-san water extract using in vitro and in vivo tests. | oryeong-san, a mixture of five herbal plants, is a well-known therapy for renal-associated diseases such as those manifesting edema, dysuria, and oliguria. | 2015 | 26268991 |
| activity-based proteomic profiling of deubiquitinating enzymes in salmonella-infected macrophages leads to identification of putative function of uch-l5 in inflammasome regulation. | although protein ubiquitination has been shown to regulate multiple processes during host response to salmonella enterica serovar typhimurium infection, specific functions of host deubiquitinating enzymes remain unknown in this bacterial infection. by using chemical proteomics approach, in which deubiquitinating enzymes were labeled by an active-site probe and analyzed by quantitative proteomics, we identified novel deubiquitinases in chicken macrophages based on their reactivity with the probe. ... | 2015 | 26267804 |
| characterization of novel factors involved in swimming and swarming motility in salmonella enterica serovar typhimurium. | salmonella enterica utilizes flagellar motility to swim through liquid environments and on surfaces. the biosynthesis of the flagellum is regulated on various levels, including transcriptional and posttranscriptional mechanisms. here, we investigated the motility phenotype of 24 selected single gene deletions that were previously described to display swimming and swarming motility effects. mutations in flge, flih, ydiv, rfag, yjcc, stm1267 and stm3363 showed an altered motility phenotype. deleti ... | 2015 | 26267246 |
| symmetry controlled, genetic presentation of bioactive proteins on the p22 virus-like particle using an external decoration protein. | viruses use spatial control of constituent proteins as a means of manipulating and evading host immune systems. similarly, precise spatial control of proteins encapsulated or presented on designed nanoparticles has the potential to biomimetically amplify or shield biological interactions. previously, we have shown the ability to encapsulate a wide range of guest proteins within the virus-like particle (vlp) from salmonella typhimurium bacteriophage p22, including antigenic proteins from human pa ... | 2015 | 26266824 |
| ulleungamides a and b, modified α,β-dehydropipecolic acid containing cyclic depsipeptides from streptomyces sp. kcb13f003. | two novel cyclic depsipeptides, ulleungamides a (1) and b (2), were isolated from cultures of terrestrial streptomyces sp. their structures were determined by analyses of spectroscopic data and various chemical transformations, including modified mosher's method, advanced marfey's method, pgme, gitc derivatizations, and snatzke's method. ulleungamides were determined to be a new class of peptides bearing unprecedented units, such as 5-hydroxy-6-methyl-2,3-dehydropipecolic acid, 4,5-dihydroxy-6-m ... | 2015 | 26262430 |
| inactivated salmonella enterica serovar typhimurium monophasic variant (s. typhimurium 1,4,[5],12:i-) in sows is effective to control infection in piglets under field condition. | the monophasic variant of salmonella enterica serovar typhimurium, namely salmonella 1,4,[5],12:i-, has been increasingly responsible for foodborne human cases of disease and is most frequently detected in pork, since the variant is widely spread in pig farms. the aim of this study was to assess the efficacy of an autologous vaccine in decreasing the prevalence of salmonella 1,4,[5],12:i-, in pigs. the trial was performed in a multisite pig production system of northern italy. the autogenous vac ... | 2015 | 26260858 |
| biofilm formation ability of salmonella enterica serovar typhimurium acrab mutants. | recent studies offer contradictory findings about the role of multidrug efflux pumps in bacterial biofilm development. thus, the aim of this study was to investigate the involvement of the acrab efflux pump in biofilm formation by investigating the ability of acrb and acrab null mutants of salmonella enterica serovar typhimurium to produce biofilms. three models were used to compare the ability of s. typhimurium wild-type and its mutants to form biofilms: formation of biofilm on polystyrene surf ... | 2015 | 26260191 |
| use of caprylic acid to control pathogens (escherichia coli o157:h7 and salmonella enterica serovar typhimurium) in apple juice at mild heat temperature. | the aim of this study was to examine the effects of caprylic acid (ca) on pathogens in apple juice having intrinsic organic acids, and to determine any synergistic effects. | 2015 | 26257401 |
| correction: correction: signatures of adaptation in human invasive salmonella typhimurium st313 populations from sub-saharan africa. | 2015 | 26252636 | |
| streptomycin induced stress response in salmonella enterica serovar typhimurium shows distinct colony scatter signature. | we investigated the streptomycin-induced stress response in salmonella enterica serovars with a laser optical sensor, bardot (bacterial rapid detection using optical scattering technology). initially, the top 20 s. enterica serovars were screened for their response to streptomycin at 100 μg/ml. all, but four s. enterica serovars were resistant to streptomycin. the mic of streptomycin-sensitive serovars (enteritidis, muenchen, mississippi, and schwarzengrund) varied from 12.5 to 50 μg/ml, while s ... | 2015 | 26252374 |
| heat-killed lactobacillus spp. cells enhance survivals of caenorhabditis elegans against salmonella and yersinia infections. | this study examined the effect of feeding heat-killed lactobacillus cells on the survival of caenorhabditis elegans nematodes after salmonella typhimurium and yersinia enterocolitica infection. the feeding of heat-killed lactobacillus plantarum 133 (lp133) and lactobacillus fermentum 21 (lp21) cells to nematodes was shown to significantly increase the survival rate as well as stimulate the expression of pmk-1 gene that key factor for c. elegans immunity upon infection compared with control nemat ... | 2015 | 26250615 |
| salmonella typhimurium strain expressing oprf-opri protects mice against fatal infection by pseudomonas aeruginosa. | pseudomonas aeruginosa poses a major threat to human health and to the mink industry. thus, development of vaccines that elicit robust humoral and cellular immunity against p. aeruginosa is greatly needed. in this study, a recombinant attenuated salmonella vaccine (rasv) that expresses the outer membrane proteins fusion oprf190-342 -opri21-83 (f1i2) from p. aeruginosa was constructed and the potency of this vaccine candidate assessed by measuring f1i2-specific humoral immune responses upon vacci ... | 2015 | 26249788 |
| structures of substrate- and nucleotide-bound propionate kinase from salmonella typhimurium: substrate specificity and phosphate-transfer mechanism. | kinases are ubiquitous enzymes that are pivotal to many biochemical processes. there are contrasting views on the phosphoryl-transfer mechanism in propionate kinase, an enzyme that reversibly transfers a phosphoryl group from propionyl phosphate to adp in the final step of non-oxidative catabolism of l-threonine to propionate. here, x-ray crystal structures of propionate- and nucleotide-bound salmonella typhimurium propionate kinase are reported at 1.8-2.0 å resolution. although the mode of nucl ... | 2015 | 26249345 |
| gold nanoparticles enhanced sers aptasensor for the simultaneous detection of salmonella typhimurium and staphylococcus aureus. | salmonella typhimurium and staphylococcus aureus are most common causes of food-associated disease. a raman based biosensor was developed for s. typhimurium and s. aureus detection simultaneously. the biosensor was based on nanoparticles enhanced raman intensity and the specific recognition of aptamer. the raman signal probe and the capture probe are built. gold nanoparticles (gnps) modified with raman molecules (mercaptobenzoic acid and 5,5'-dithiobis(2-nitrobenzoic acid)) and aptamer are used ... | 2015 | 26241735 |
| tumor-targeting salmonella typhimurium a1-r arrests a chemo-resistant patient soft-tissue sarcoma in nude mice. | a patient-derived nude-mouse model of soft-tissue sarcoma has been established and treated in the following groups: (1) untreated controls; (2) gemcitabine (gem) (80 mg/kg, ip, weekly, 3 weeks); (3) pazopanib (100 mg/kg, orally, daily, 3 weeks) and (4) salmonella typhimurium a1-r (5 × 10(7) cfu/body, ip, weekly, 3 weeks). the sarcoma was resistant to gem (p = 0.879). pazopanib tended to reduce the tumor volume compared to the untreated mice, but there was no significant difference (p = 0.115). s ... | 2015 | 26237416 |
| tailored delivery of analgesic ziconotide across a blood brain barrier model using viral nanocontainers. | the blood brain barrier (bbb) is often an insurmountable obstacle for a large number of candidate drugs, including peptides, antibiotics, and chemotherapeutic agents. devising an adroit delivery method to cross the bbb is essential to unlocking widespread application of peptide therapeutics. presented here is an engineered nanocontainer for delivering peptidic drugs across the bbb encapsulating the analgesic marine snail peptide ziconotide (prialt®). we developed a bi-functional viral nanocontai ... | 2015 | 26234920 |
| monitoring the incidence and causes of diseases potentially transmitted by food in australia: annual report of the ozfoodnet network, 2011. | this report summarises the incidence of diseases potentially transmitted by food in australia and details outbreaks associated with food in 2011. ozfoodnet sites reported 30,957 notifications of 9 diseases or conditions that may be transmitted by food. the most commonly notified infections were campylobacter (17,733 notifications) followed by salmonella (12,271 notifications). the most frequently notified salmonella serotype was salmonella typhimurium, accounting for 48% of all salmonella notifi ... | 2015 | 26234259 |
| regulation and function of the salmonella mgtc virulence protein. | salmonella enterica serovar typhimurium produces many virulence proteins to cause diseases. the salmonella mgtc protein is one of such virulence proteins specially required for intracellular proliferation inside macrophages and mouse virulence. in this review, we will cover how the mgtc gene is turned on or off and what the signals required for mgtc expression are. later in this review, we will discuss a recent understanding of mgtc function in salmonella pathogenesis by identifying its target p ... | 2015 | 26231375 |
| proteomics evidence for the activity of the putative antibacterial plant alkaloid (-)-roemerine: mainstreaming omics-guided drug discovery. | discovery of new antibacterials with novel mechanisms is important to counteract the ingenious resistance mechanisms of bacteria. in this connection, omics-guided drug discovery offers a rigorous method in the quest of new antibacterials. (-)-roemerine is a plant alkaloid that has been reported to possess putative antibacterial activity against escherichia coli, bacillus subtilis, and salmonella typhimurium. the aim of the present study was to characterize the activity of (-)-roemerine in escher ... | 2015 | 26230533 |
| immunological study of an attenuated salmonella typhimurium expressing apxia, apxiia, apxiiia and ompa of actinobacillus pleuropneumoniae in a mouse model. | salmonella typhimurium strain expressing the actinobacillus pleuropneumoniae antigens, apxia, apxiia, apxiiia and ompa, was previously constructed as a vaccine candidate for porcine pleuropneumonia. this strain was a live attenuated (∆lon∆cpxr∆asd)salmonella as a delivery host and contained a vector containing asd. an immunological study of lymphocyte proliferation, t-lymphocyte subsets and cytokines in the splenocytes of a mouse model was carried out after stimulation with the candidate salmone ... | 2016 | 26227587 |
| methionine sulfoxide reductase a (msra) contributes to salmonella typhimurium survival against oxidative attack of neutrophils. | salmonella typhimurium (st) must evade neutrophil assault for infection establishment in the host. myeloperoxidase generated hocl is the key antimicrobial agent produced by the neutrophils; and methionine (met) residues are the primary targets of this oxidant. oxidation of mets leads to methionine sulfoxide (met-so) formation and consequently compromises the protein function(s). methionine sulfoxide reductase a (msra) reductively repairs met-so to mets. in this manner, msra maintains the functio ... | 2015 | 26224245 |
| intestinal inflammation leads to a long-lasting increase in resistance to systemic salmonellosis that requires macrophages but not b or t lymphocytes at the time of pathogen challenge. | intestinal inflammation is associated with systemic translocation of commensal antigens and the consequent activation of b and t lymphocytes. the long-term consequences of such immune activation are not completely understood. | 2015 | 26222341 |
| distribution and molecular characterization of salmonella enterica hypermutators in retail food in china. | hypermutable pathogens can easily acquire mutation opportunities, as well as antimicrobial resistance, and are tremendous hazards to food safety and public health. in this study, a total of 96 (7.6%) hypermutators were identified from 1,264 salmonella isolates recovered from retail foods. pulsed-field gel electrophoresis analysis indicated that hypermutators were genetically diverse. amino acid substitution of val421phe was detected in muts in one hypermutator and val246ala in 56 other hypermuta ... | 2015 | 26219361 |
| in vitro synergistic effect of the cm11 antimicrobial peptide in combination with common antibiotics against clinical isolates of six species of multidrug-resistant pathogenic bacteria. | during the last decades, increase of antibiotic resistance among pathogenic bacteria has been considered as a global concern. therefore, it is important to find new antimicrobial agents and/or therapeutic strategies. in previous studies we investigated antibacterial activity of the cm11 peptide against multiple drug resistant clinical isolates of six bacteria species including pseudomonas aeruginosa, staphylococcus aureus, acinetobacter baumannii, escherichia coli, klebsiella pneumoniae and salm ... | 2015 | 26216264 |
| a tpl2 (map3k8) disease-risk polymorphism increases tpl2 expression thereby leading to increased pattern recognition receptor-initiated caspase-1 and caspase-8 activation, signalling and cytokine secretion. | ibd is characterised by dysregulated intestinal immune homeostasis and cytokine secretion. in the intestine, properly regulating pattern recognition receptor (prr)-mediated signalling and cytokines is crucial given the ongoing host-microbial interactions. tpl2 (map3k8, cot) contributes to prr-initiated pathways, yet the mechanisms for tpl2 signalling contributions in primary human myeloid cells are incompletely understood and its role in intestinal myeloid cells is poorly defined. furthermore, f ... | 2016 | 26215868 |
| polyphenols rich fraction from geoffroea decorticans fruits flour affects key enzymes involved in metabolic syndrome, oxidative stress and inflammatory process. | geoffroea decorticans (chañar), is widely distributed throughout northwestern argentina. its fruit is consumed as flour, arrope or hydroalcoholic beverage. the chañar fruits flour was obtained and 39 phenolic compounds were tentatively identified by hplc-ms/ms(n). the compounds comprised caffeic acid glycosides, simple phenolics (protocatechuic acid and vanillic acid), a glycoside of vanillic acid, p-coumaric acid and its phenethyl ester as well as free and glycosylated flavonoids. the polypheno ... | 2016 | 26212988 |
| [outbreak of salmonella typhimurium infections associated with consumption of chorizo in bizkaia]. | a report is presented on an outbreak of salmonella enterica serovar typhimurium infection that affected six people. the epidemiological and laboratory investigation associated the outbreak with the consumption of homemade chorizo purchased at a local street market. the vendor and producer were informed that the sale of meat products without sanitary authorization is prohibited, and the product was withdrawn from sale. | 2016 | 26211840 |
| salmonella occurrence and enterobacteriaceae counts in pig feed ingredients and compound feed from feed mills in ireland. | the purpose of this study was to assess the occurrence of non-typhoidal salmonellae and enterobacteriaceae counts in raw ingredients and compound feeds sampled from feed mills manufacturing pig diets. between november 2012 and september 2013, feed ingredients (n=340) and compound pig feed (n=313) samples were collected from five commercial feed mills and one home compounder at various locations throughout ireland. feed ingredients included cereals, vegetable protein sources and by-products of oi ... | 2015 | 26211839 |
| exposure of salmonella enterica serovar typhimurium to three humectants used in the food industry induces different osmoadaptation systems. | common salt (nacl) is frequently used by the food industry to add flavor and to act as a humectant in order to reduce the water content of a food product. the improved health awareness of consumers is leading to a demand for food products with reduced salt content; thus, manufacturers require alternative water activity-reducing agents which elicit the same general effects as nacl. two examples include kcl and glycerol. these agents lower the water activity of a food matrix and also contribute to ... | 2015 | 26209672 |
| investigation of nalidixic acid resistance mechanism in salmonella enterica using molecular simulation techniques. | the emergence of nalidixic acid-resistant strains of salmonella typhimurium remains to be a major public health problem. in particular, the substitution of asn in place of asp at the 87 loci in the gyra of s. typhimurium was experimentally stated for nalidixic acid resistance. however, the data on the possible mechanism of nalidixic acid resistance are limited. in this study, i-mutant2.0 and duet program were employed to explore the impact of mutation on the stability of gyra protein. subsequent ... | 2015 | 26208690 |
| effect of dietary nonphytate phosphorus content on ileal lymphocyte subpopulations and cytokine expression in the cecal tonsils and spleen of laying hens that were or were not orally inoculated with salmonella typhimurium. | to evaluate the effects of dietary nonphytate phosphorus (npp) content on ileal lymphocyte subpopulations and cytokine expression in the cecal tonsils and spleen of hens that were or were not inoculated with salmonella typhimurium. | 2015 | 26207969 |
| vasoactive intestinal peptide (vip) differentially affects inflammatory immune responses in human monocytes infected with viable salmonella or stimulated with lps. | we compared the effect of vip on human blood monocytes infected with salmonella typhimurium 4/74 or stimulated with lps. vip (10(-7)m) increased monocyte viability by 24% and 9% when cultured for 24h with 4/74 or salmonella lps (100ng/ml), respectively. significantly increased (p<0.05) numbers of 4/74 were also recovered from monocytes co-cultured with vip after 6h post-infection (pi) and this remained high after 24h pi. both 4/74 and lps increased (p<0.05) the concentration of tnf-α, il-1β and ... | 2015 | 26206287 |
| a multiplex oligonucleotide ligation-pcr as a complementary tool for subtyping of salmonella typhimurium. | subtyping below the serovar level is essential for surveillance and outbreak detection and investigation of salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) and its monophasic variant 1,4,[5],12:i:- (s. 1,4,[5],12:i:-), frequent causes of foodborne infections. in an attempt to overcome the intrinsic shortcomings of currently used subtyping techniques, a multiplex oligonucleotide ligation-pcr (mol-pcr) assay was developed which combines different types of molecular markers ... | 2015 | 26205523 |
| microencapsulated sorbic acid and pure botanicals affect salmonella typhimurium shedding in pigs: a close-up look from weaning to slaughter in controlled and field conditions. | the aim of this study was to assess the efficacy of a combination of sorbic acid, thymol, and carvacrol in reducing the prevalence and shedding level of salmonella typhimurium in pigs either in a controlled challenge environment or in a production setting. in the first study, 24 weaned piglets were separated in 4 isolation units (6 piglets/isolation unit). each unit received either a basal diet (no treatment) or a microencapsulated mixture of sorbic acid, thymol, and carvacrol at 1, 2, or 5 g/kg ... | 2015 | 26203634 |
| salmonella typhimurium co-opts the host type i ifn system to restrict macrophage innate immune transcriptional responses selectively. | innate immune inflammatory responses are subject to complex layers of negative regulation at intestinal mucosal surfaces. although the type i ifn system is critical for amplifying antiviral immunity, it has been shown to play a homeostatic role in some models of autoimmune inflammation. type i ifn is triggered in the gut by select bacterial pathogens, but whether and how the type i ifn might regulate innate immunity in the intestinal environment have not been investigated in the context of salmo ... | 2015 | 26202980 |
| combination of dynamic magnetophoretic separation and stationary magnetic trap for highly sensitive and selective detection of salmonella typhimurium in complex matrix. | foodborne illnesses have always been a serious problem that threats public health, so it is necessary to develop a method that can detect the pathogens rapidly and sensitively. in this study, we designed a magnetic controlled microfluidic device which integrated the dynamic magnetophoretic separation and stationary magnetic trap together for sensitive and selective detection of salmonella typhimurium (s. typhimurium). coupled with immunomagnetic nanospheres (imns), this device could separate and ... | 2015 | 26201979 |
| rescuing chemotaxis of the anticancer agent salmonella enterica serovar typhimurium vnp20009. | the role of chemotaxis and motility in salmonella enterica serovar typhimurium tumor colonization remains unclear. we determined through swim plate assays that the well-established anticancer agent s. typhimurium vnp20009 is deficient in chemotaxis, and that this phenotype is suppressible. through genome sequencing, we revealed that vnp20009 and four selected suppressor mutants had a single nucleotide polymorphism (snp) in chey causing a mutation in the conserved proline residue at position 110. ... | 2015 | 26200833 |
| microarray on digital versatile disc for identification and genotyping of salmonella and campylobacter in meat products. | highly portable, cost-effective, and rapid-response devices are required for the subtyping of the most frequent food-borne bacteria; thereby the sample rejection strategies and hygienization techniques along the food chain can be tailor-designed. here, a novel biosensor is presented for the generic detection of salmonella and campylobacter and the discrimination between their most prevalent serovars (salmonella enteritidis, salmonella typhimurium) and species (campylobacter jejuni, campylobacter ... | 2015 | 26198111 |
| efficacy of lytic bacteriophage preparation in reducing salmonella in vitro, on turkey breast cutlets, and on ground turkey. | the efficacy of the recently approved salmonella lytic bacteriophage preparation (salmofresh) in reducing salmonella enterica serotype heidelberg on turkey breast cutlets and ground turkey was evaluated. in a broth model assay, the phage preparation completely inhibited the growth of four s. enterica serotypes (salmonella enteritidis, salmonella heidelberg, salmonella kentucky, and salmonella typhimurium) at 37°c at a multiplicity of infection of 10,000 pfu/cfu. at 4°c in 0.1% peptone water (pw) ... | 2015 | 26197288 |
| the o-antigen capsule of salmonella enterica serovar typhimurium facilitates serum resistance and surface expression of flic. | group iv polysaccharide capsules are common in enteric bacteria and have more recently been described in nontyphoidal salmonella species. such capsules are known as o-antigen (o-ag) capsules, due to their high degree of similarity to the o-ag of the lipopolysaccharide (lpso-ag). capsular polysaccharides are known virulence factors of many bacterial pathogens, facilitating evasion of immune recognition and systemic dissemination within the host. previous studies on the o-ag capsule of salmonellae ... | 2015 | 26195553 |
| inhibition of pathogenic bacterial growth on excision wound by green synthesized copper oxide nanoparticles leads to accelerated wound healing activity in wistar albino rats. | an impaired wound healing is one of the major health related problem in diabetic and non-diabetic patients around the globe. the pathogenic bacteria play a predominant role in delayed wound healing, owing to interaction in the wound area. in our previous work we developed green chemistry mediated copper oxide nanoparticles using ficus religiosa leaf extract. in the present study we make an attempt to evaluate the anti-bacterial, and wound healing activity of green synthesized copper oxide nanopa ... | 2015 | 26194977 |
| characterization of salmonella enterica serovar 4,[5],12:i:- isolates from korean food animals. | salmonella enterica subspecies enterica serovar 4,[5],12:i:-, a monophasic variant of salmonella typhimurium, has emerged as one of the most common serotypes related to human salmonellosis. in this study, the 22 isolates of s. 4,[5],12:i:- from food animals were identified by a specific multiplex polymerase chain reaction between 2009 and 2012. the isolation rate of s. 4,[5],12:i:- accounted for 1.7% (22/1271) of salmonella spp. isolates from food animal origins: more specifically, 7.6% (18/235) ... | 2015 | 26192872 |
| iron regulatory proteins mediate host resistance to salmonella infection. | macrophages are essential for systemic iron recycling, and also control iron availability to pathogens. iron metabolism in mammalian cells is orchestrated posttranscriptionally by iron-regulatory proteins (irp)-1 and -2. here, we generated mice with selective and combined ablation of both irps in macrophages to investigate the role of irps in controlling iron availability. these animals are hyperferritinemic but otherwise display normal clinical iron parameters. however, mutant mice rapidly succ ... | 2015 | 26190773 |
| microbiological toxicity of tilmicosin on human colonic microflora in chemostats. | to evaluate the microbiological safety of tilmicosin on human intestinal microflora, four chemostat models of healthy human colonic ecosystems were exposed to tilmicosin (0, 0.436, 4.36, and 43.6 μg/ml) for 7 days. prior to and during drug exposure, three microbiological endpoints were monitored daily including short-chain fatty acids, bacterial counts and macrolide susceptibility. colonization resistance of each community was determined by 3 successive daily challenges of salmonella typhimurium ... | 2015 | 26190303 |
| comparative cytotoxic and genotoxic potential of 13 drinking water disinfection by-products using a microplate-based cytotoxicity assay and a developed sos/umu assay. | the implications of disinfection by-products (dbps) present in drinking water are of public health concern because of their potential mutagenic, carcinogenic and other toxic effects on humans. in this study, we selected 13 main dbps found in drinking water to quantitatively analyse their cytotoxicity and genotoxicity using a microplate-based cytotoxicity assay and a developed sos/umu assay in salmonella typhimurium ta1535/psk1002. with the developed sos/umu test, eight dbps: 3-chloro-4-(dichloro ... | 2016 | 26188195 |
| evaluation of in vitro and in vivo safety profile of the indian traditional medicinal plant grewia tiliaefolia. | grewia tiliaefolia vahl., is a subtropical tree used in ayurvedic formulations and also by tribal communities in india for treatment of wounds, urinary infection and skin diseases. despite of its medicinal use, the plant has not been previously tested for its safety. this study evaluated the toxicological profile of the methanolic extract of g. tiliaefolia leaves (megt) through in vitro (cytotoxic, mutagenic, genotoxic) and in vivo (acute and sub-acute) studies. in vitro assays conclude that the ... | 2015 | 26188119 |
| cold plasma treatment for the microbiological safety of cabbage, lettuce, and dried figs. | microwave-powered cold plasma treatment (cpt) was evaluated as a means to improve the microbiological safety of fresh vegetables and dried fruits. the cpt at 900 w, conducted for 10 min using nitrogen as a plasma-forming gas, inactivated salmonella typhimurium inoculated on cabbage and lettuce by approximately 1.5 log cfu/g. the cpt at 400-900 w and 667 pa, conducted for 1-10 min using a helium-oxygen gas mixture, inactivated listeria monocytogenes on cabbage by 0.3-2.1 log cfu/g in a time-depen ... | 2015 | 26187830 |
| crystal structure of β-n-acetylglucosaminidase cbsa from thermotoga neapolitana. | cbsa from the thermophilic marine bacteria thermotoga neapolitana is a chitinolyitc enzyme that can cleave a glycosidic bond of the polymer n-acetylglucosamine at the non-reducing end. this enzyme has particularly high activity on di-n-acetylchitobiose. cbsa consists of a family of 3 glycoside hydrolase (gh3)-type catalytic domains and a unique c-terminal domain. the c-terminal domain distinguishes cbsa from other gh3-type enzymes. sequence analyses have suggested that cbsa has the asp-his dyad ... | 2015 | 26187666 |
| salmonella infection drives promiscuous b cell activation followed by extrafollicular affinity maturation. | the b cell response to salmonella typhimurium (stm) occurs massively at extrafollicular sites, without notable germinal centers (gcs). little is known in terms of its specificity. to expand the knowledge of antigen targets, we screened plasmablast (pb)-derived monoclonal antibodies (mabs) for salmonella specificity, using elisa, flow cytometry, and antigen microarray. only a small fraction (0.5%-2%) of the response appeared to be salmonella-specific. yet, infection of mice with limited b cell re ... | 2015 | 26187411 |
| correction: granulocytes impose a tight bottleneck upon the gut luminal pathogen population during salmonella typhimurium colitis. | 2015 | 26186334 | |
| from pasteur to probiotics: a historical overview of cheese and microbes. | cheese is a food which has been produced for centuries. while cheese was originally developed as a product which extended the shelf life of milk, over time distinct cheese varieties arose, being shaped by geographic, climate, cultural, and economic factors. global demand for artisan cheeses is creating new economic opportunities. consumers seeking distinctive products with regional flavor, or terroir, are becoming connoisseurs of hand-crafted cheeses with distinctive tastes and character. these ... | 2013 | 26184821 |
| laminarin from irish brown seaweeds ascophyllum nodosum and laminaria hyperborea: ultrasound assisted extraction, characterization and bioactivity. | ultrasound assisted extraction (uae), purification, characterization and antioxidant activity of laminarin from irish brown seaweeds ascophyllum nodosum and laminarina hyperborea were investigated. uae was carried out using 60% ultrasonic power amplitude and 0.1 m hydrochloric acid for 15 min. separately, solid-liquid extraction was carried in an orbital shaker using 0.1 m hydrochloric acid at 70 °c for 2.5 h. uae with hydrochloric acid resulted in the highest concentration of laminarin, 5.82% a ... | 2015 | 26184235 |
| understanding the complexities of salmonella-host crosstalk as revealed by in vivo model organisms. | foodborne infections caused by non-typhoidal salmonellae, such as salmonella enterica serovar typhimurium (st), pose a major challenge in the developed and developing world. with constant rise of drug-resistant strains, understanding the epidemiology, microbiology, pathogenesis and host-pathogen interactions biology is a mandatory requirement to enable health systems to be ready to combat these illnesses. patient data from hospitals, at least from some parts of the world, have aided in epidemiol ... | 2015 | 26179888 |
| evaluation of a water extract of so-cheong-ryong-tang for acute toxicity and genotoxicity using in vitro and in vivo tests. | so-cheong-ryong-tang, a traditional korean medicine, has been used empirically for the treatment of asthma, allergic rhinitis, and colds for hundreds of years. however, its genotoxicity has been rarely examined. | 2015 | 26178777 |
| blue 470 nm light suppresses the growth of salmonella enterica and methicillin-resistant staphylococcus aureus (mrsa) in vitro. | emerging evidence suggests that blue light can photo-inactivate some bacteria of clinical importance. consequently, we tested the hypothesis that 470 nm light can suppress growth of two recalcitrant bacteria, mrsa and salmonella. | 2015 | 26174877 |
| nlrp3 inflammasome activation downstream of cytoplasmic lps recognition by both caspase-4 and caspase-5. | humans encode two inflammatory caspases that detect cytoplasmic lps, caspase-4 and caspase-5. when activated, these trigger pyroptotic cell death and caspase-1-dependent il-1β production; however the mechanism underlying this process is not yet confirmed. we now show that a specific nlrp3 inhibitor, mcc950, prevents caspase-4/5-dependent il-1β production elicited by transfected lps. given that both caspase-4 and caspase-5 can detect cytoplasmic lps, it is possible that these proteins exhibit som ... | 2015 | 26173988 |
| structural features reminiscent of atp-driven protein translocases are essential for the function of a type iii secretion-associated atpase. | many bacterial pathogens and symbionts utilize type iii secretion systems to interact with their hosts. these machines have evolved to deliver bacterial effector proteins into eukaryotic target cells to modulate a variety of cellular functions. one of the most conserved components of these systems is an atpase, which plays an essential role in the recognition and unfolding of proteins destined for secretion by the type iii pathway. here we show that structural features reminiscent of other atp-d ... | 2015 | 26170413 |
| gastroprotective potential against indomethacin and safety assessment of the homology of medicine and food formula cuttlebone complex. | cuttlebone complex (cbc), a homology of medicine and food formula, is comprised of five herbal medicines (endoconcha sepiae, radix paeoniae rubra, fresh ginger, fructus amomi, and radix glycyrrhizae) and two food ingredients (zingiber zerumbet and chitosan). herein, the gastroprotective potential against indomethacin and a safety assessment of cbc were investigated. in a gastroprotective model, cbc effectively decreased the indomethacin-increased gastric ulcerous lesions, and increased the indom ... | 2015 | 26169959 |
| plasma deposition of silver nanoparticles on ultrafiltration membranes: antibacterial and anti-biofouling properties. | a novel and versatile plasma reactor was used to modify polyethersulphone commercial membranes. the equipment was applied to: i) functionalize the membranes with low-temperature plasmas, ii) deposit a film of poly(methyl methacrylate) (pmma) by plasma enhanced chemical vapor deposition (pecvd) and, iii) deposit silver nanoparticles (snp) by gas flow sputtering. each modification process was performed in the same reactor consecutively, without exposure of the membranes to atmospheric air. scannin ... | 2015 | 26166926 |
| induction of immunocastration in pre-pubertal boars immunized with recombinant gonadotropin-releasing hormone conjugated with salmonella typhimurium flagellin fljb. | immunocastration is an alternative method used to replace surgical castration commonly performed in swine farms. in boars, the main effects of immunocastration are reduction of gonadotropin-releasing hormone (gnrh) and the resulting inhibition of testicular function. the aim of this study was to evaluate immunocastration efficacy in pre-pubertal boars vaccinated with a recombinant gnrh protein conjugated with salmonella typhimurium flagellin fljb (stf2). a total of 35 boars were assigned to thre ... | 2015 | 26164876 |
| fundamental characteristics of deep-uv light-emitting diodes and their application to control foodborne pathogens. | low-pressure mercury uv (lp-uv) lamps have long been used for bacterial inactivation, but due to certain disadvantages, such as the possibility of mercury leakage, deep-uv-c light-emitting diodes (duv-leds) for disinfection have recently been of great interest as an alternative. therefore, in this study, we examined the basic spectral properties of duv-leds and the effects of uv-c irradiation for inactivating foodborne pathogens, including escherichia coli o157:h7, salmonella enterica serovar ty ... | 2015 | 26162872 |
| attenuated mutant strain of salmonella typhimurium lacking the znuabc transporter contrasts tumor growth promoting anti-cancer immune response. | salmonella typhimurium has been shown to be highly effective as antitumor agent. the aim of this study was to investigate the tumor targeting efficacy and the mechanism of action of a specific attenuated mutant strain of salmonella typhimurium (stm) devoid of the whole operon coding for the high-affinity zinc transporter znuabc, which is required for bacterial growth in environments poor in zinc and for conferring full virulence to different gram-negative pathogens.we showed that stm is able to ... | 2015 | 26158862 |
| efficacy of neutral ph electrolyzed water in reducing escherichia coli o157:h7 and salmonella typhimurium dt 104 on fresh produce items using an automated washer at simulated food service conditions. | the objective of this study was to determine the efficacy of neutral ph electrolyzed (neo) water (155 mg/l free chlorine, ph 7.5) in reducing escherichia coli o157:h7 and salmonella typhimurium dt 104 on romaine lettuce, iceberg lettuce, and tomatoes washed in an automated produce washer for different times and washing speeds. tomatoes and lettuce leaves were spot inoculated with 100 μl of a 5 strain cocktail mixture of either pathogen and washed with 10 or 8 l of neo water, respectively. washin ... | 2015 | 26155998 |
| synthesis, in vitro and in silico studies of some novel 5-nitrofuran-2-yl hydrazones as antimicrobial and antitubercular agents. | in this study, we synthesized two series of novel 5-nitrofuran-2-carbohydrazides 21a-h and 22a-e in addition to a third series of thiophene-2-carbohydrazides 23a-g to develop potent antimicrobial and/or antitubercular agents. the newly synthesized compounds were evaluated in vitro for their antimicrobial and antimycobacterial activities. most of the 5-nitrofuran-2-carbohydrazides 21a-h and 22a-e displayed variable activity against aspergillus fumigates, staphylococcus aureus, streptococcus pneum ... | 2015 | 26155871 |
| factors affecting pathogen survival in finished dairy compost with different particle sizes under greenhouse conditions. | this study investigated the survival of escherichia coli o157:h7 and salmonella typhimurium in finished dairy compost with different particle sizes during storage as affected by moisture content and temperature under greenhouse conditions. the mixture of e. coli o157:h7 and s. typhimurium strains was inoculated into the finished composts with moisture contents of 20, 30, and 40%, separately. the finished compost samples were then sieved into 3 different particle sizes (>1000, 500-1000, and <500 ... | 2015 | 26153914 |
| stool bacteriomic profiling in patients with metastatic renal cell carcinoma receiving vascular endothelial growth factor-tyrosine kinase inhibitors. | diarrhea occurs in approximately half of patients with metastatic renal cell carcinoma (mrcc) receiving vascular endothelial growth factor-tyrosine kinase inhibitors (vegf-tki). we evaluated the relationship between vegf-tki-related diarrhea and stool microbiota. | 2015 | 26152743 |
| functional analysis of a novel cysteine-rich antimicrobial peptide from the salivary glands of the tick rhipicephalus haemaphysaloides. | ticks encounter various microbes while sucking blood from an infected host and carrying these pathogens in themselves. ticks can then transmit these pathogens to vertebrate hosts. the immune system of ticks can be stimulated to produce many bioactive molecules during feeding and pathogen invasion. antimicrobial peptides (amps) are key effector molecules of a tick's immune response, as they can kill invading pathogenic microorganisms. in this study, we identified a novel cysteine-rich amp, design ... | 2015 | 26152423 |
| recombinant salmonella expressing burkholderia mallei lps o antigen provides protection in a murine model of melioidosis and glanders. | burkholderia pseudomallei and burkholderia mallei are the etiologic agents of melioidosis and glanders, respectively. these bacteria are highly infectious via the respiratory route and can cause severe and often fatal diseases in humans and animals. both species are considered potential agents of biological warfare; they are classified as category b priority pathogens. currently there are no human or veterinary vaccines available against these pathogens. consequently efforts are directed towards ... | 2015 | 26148026 |
| identification of salmonella typhimurium deubiquitinase ssel substrates by immunoaffinity enrichment and quantitative proteomic analysis. | ubiquitination is a key protein post-translational modification that regulates many important cellular pathways and whose levels are regulated by equilibrium between the activities of ubiquitin ligases and deubiquitinases. here, we present a method to identify specific deubiquitinase substrates based on treatment of cell lysates with recombinant enzymes, immunoaffinity purification, and global quantitative proteomic analysis. as a model system to identify substrates, we used a virulence-related ... | 2015 | 26147956 |
| in vitro probiotic characteristics of lactobacillus plantarum zdy 2013 and its modulatory effect on gut microbiota of mice. | lactobacillus plantarum zdy 2013, a novel strain isolated from chinese traditional fermented acid beans, was systematically evaluated for its survival capacity under stress conditions (ph, bile salt, simulated gastrointestinal tract, and antibiotics), production of exopolysaccharide and antagonism against 8 pathogens. its effect on mice gut microbiota was also investigated by quantitative pcr and pcr-denaturing gradient gel electrophoresis. the results showed that zdy 2013 can grow at ph 3.5 and ... | 2015 | 26142853 |
| electrospun antimicrobial hybrid mats: innovative packaging material for meat and meat-products. | to prevent the development and spread of spoilage/pathogenic microorganisms via meat foodstuffs, antimicrobial nanocomposite packaging can serve as a potential alternative. the objective of this study was to develop a new class of antimicrobial hybrid packaging mat composed of biodegradable polyurethane supplemented with virgin olive oil and zinc oxide via electrospinning. instead of mixing antimicrobial compounds directly with food, incorporation in packaging materials allows the functional eff ... | 2015 | 26139931 |
| selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (gtg)5-pcr fingerprinting. | the aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (lab) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. antimicrobial activity of lab was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of bacillus cereus, listeria monocytogenes, escherichia coli, staphy ... | 2014 | 26139877 |
| expression of the blood-group-related gene b4galnt2 alters susceptibility to salmonella infection. | glycans play important roles in host-microbe interactions. tissue-specific expression patterns of the blood group glycosyltransferase β-1,4-n-acetylgalactosaminyltransferase 2 (b4galnt2) are variable in wild mouse populations, and loss of b4galnt2 expression is associated with altered intestinal microbiota. we hypothesized that variation in b4galnt2 expression alters susceptibility to intestinal pathogens. to test this, we challenged mice genetically engineered to express different b4galnt2 tiss ... | 2015 | 26133982 |
| evaluation of synergistic antibacterial and antioxidant efficacy of essential oils of spices and herbs in combination. | the present study was carried out to evaluate the possible synergistic interactions on antibacterial and antioxidant efficacy of essential oils of some selected spices and herbs [bay leaf, black pepper, coriander (seed and leaf), cumin, garlic, ginger, mustard, onion and turmeric] in combination. antibacterial combination effect was evaluated against six important food-borne bacteria (bacillus cereus, listeria monocytogenes, micrococcus luteus, staphylococcus aureus, escherichia coli and salmone ... | 2015 | 26132146 |
| characterization of salmonella typhimurium isolates from domestically acquired infections in finland by phage typing, antimicrobial susceptibility testing, pfge and mlva. | salmonella enterica spp. enterica serotype typhimurium (stm) is the most common agent of domestically acquired salmonellosis in finland. subtyping methods which allow the characterization of stm are essential for effective laboratory-based stm surveillance and for recognition of outbreaks. this study describes the diversity of finnish stm isolates using phage typing, antimicrobial susceptible testing, pulsed-field gel electrophoresis (pfge) and multilocus variable-number tandem repeat analysis ( ... | 2015 | 26129826 |