Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| time for a fresh look at the bacterial chromosome. | 1999 | 10366858 | |
| [neurological manifestations indicative of brucellosis]. | eight patients presented neurological signs secondary to brucella infection. the clinical presentation was a meningoencephalitis in three cases, a meningoencephalomyelitis in one case, an epiduritis with spinal cord compression in one case, an acute polyradiculoneuritis in two cases and a chronic polyradiculoneuritis in one case. acoustic nerve was impaired in seven cases. cerebrospinal fluid (csf) analysis revealed a lymphocytic meningitis and a high protein concentration in all cases. the aggl ... | 1999 | 10339791 |
| molecular characterization of a brucella species large dna fragment deleted in brucella abortus strains: evidence for a locus involved in the synthesis of a polysaccharide. | a brucella melitensis 16m dna fragment of 17,119 bp, which contains a large region deleted in b. abortus strains and dna flanking one side of the deletion, has been characterized. in addition to the previously identified omp31 gene, 14 hypothetical genes have been identified in the b. melitensis fragment, most of them showing homology to genes involved in the synthesis of a polysaccharide. considering that 10 of the 15 genes are missing in b. abortus and that all the polysaccharides described in ... | 1999 | 10338472 |
| effect of early antibiotic treatment on the antibody response to cytoplasmic proteins of brucella melitensis in mice. | to test whether antibiotic therapy hampers the antibody response to brucella antigens, 30 balb/c mice were infected with brucella melitensis h38 and randomized for treatment with doxycycline administered intraperitoneally for 42 days starting at 7 or 28 days postinfection (p.i.) (groups dox7 and dox28, respectively) or for no treatment (control group). antibodies to smooth lipopolysaccharide (lps) reached peak levels (mean optical density [od] = 2.618) between days 56 and 70 p.i. in the control ... | 1999 | 10225853 |
| in vitro activities of antibiotics alone and in combination against brucella melitensis at neutral and acidic phs. | brucellae survive acidic phs in phagolysosomes. azithromycin, streptomycin, and quinolones were active against brucella melitensis at ph 7.0 but not at ph 5.0; rifampin and doxycycline retained activity at ph 5.0. regardless of ph, azithromycin-rifampin and ofloxacin-rifampin showed less synergy than established streptomycin-doxycycline and rifampin-doxycycline combinations. | 1999 | 10223958 |
| [development of a method for alkaline extraction of dna from brucella for diagnosing brucellosis using the polymerase chain reaction]. | several methods of alkaline extraction of chromosome dna from brucella in the presence of 50 microliters model diagnostic material blood serum are developed for the diagnosis of brucellosis by the polymerase chain reaction (pcr). these methods are based on the capacity of naoh to effectively denature proteins and destroy brucella cell wall, thus isolating the genome dna without exposure to proteolytic enzymes, detergents, deproteinization, or ph neutralization. the first method consists in alkal ... | 1999 | 10190109 |
| brucella melitensis 16m: characterisation of the gale gene and mouse immunisation studies with a gale deficient mutant. | the gale gene of streptomyces lividans was used to probe a cosmid library harbouring brucella melitensis 16m dna and the nucleotide sequence of a 2.5 kb clai fragment which hybridised was determined. an open reading frame encoding a predicted polypeptide with significant homology to udp-galactose-4-epimerases of brucella arbortus strain 2308 and other bacterial species was identified. dna sequences flanking the b. melitensis gale gene shared no identity with other gal genes and, as for b. abortu ... | 1999 | 10068125 |
| performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis. | competitive and standard enzyme-linked immunosorbent assays (elisas), rose bengal (rb), complement fixation, and agar gel immunoprecipitation with native hapten (agid-nh) were compared by using sera from brucella-free, brucella melitensis-infected, and b. melitensis rev1-vaccinated sheep. the most sensitive tests were indirect elisa and rb, and the most specific tests were agid-nh and competitive elisa. we show that rb followed by agid-nh is a simple and effective system for diagnosing sheep bru ... | 1999 | 10066666 |
| differentiation of microorganisms based on pyrolysis-ion trap mass spectrometry using chemical ionization. | the ability to differentiate microorganisms using pyrolysision trap mass spectrometry was demonstrated for five gram-negative disease-causing organisms: brucella melitensis, brucella suis, vibrio cholera, yersinia pestis, and francisella tularensis. bacterial profiles were generated for gamma-irradiated bacterial samples using pyrolytic methylation and compared for electron ionization and chemical ionization using several liquid reagents with increasing proton affinities. electron ionization com ... | 1999 | 9989380 |
| immunogenicity of recombinant escherichia coli expressing the omp31 gene of brucella melitensis in balb/c mice. | balb/c mice were immunized with recombinant escherichia coli expressing the omp31 gene of brucella melitensis, a gene coding for a major outer membrane protein. immunization resulted in the production of specific antibodies to b. melitensis in the serum, the production of which was considerably increased after boosting with a dose ten times lower than the first. a significant specific proliferative response of immune spleen cells to b. melitensis was observed 5 weeks after the first immunization ... | 1999 | 9987174 |
| leukocytoclastic vasculitis as a cutaneous manifestation of systemic infection caused by brucella melitensis. | a 22-year-old man with leukocytoclastic vasculitis as a cutaneous manifestation of brucellosis is presented. skin lesions resolved 48 hours after starting treatment with tetracycline and rifampicin. although skin manifestations are rarely associated with infection due to brucella and do not seem to worsen the prognosis, they may cause the patient to seek medical attention as well as contribute to the diagnosis. | 1999 | 9951590 |
| quantitative assessment by flow cytometry of t-lymphocytes producing antigen-specific gamma-interferon in brucella immune cattle. | peripheral blood mononuclear cells (pbmc) isolated from cattle infected with brucella secreted gamma-interferon (ifn-gamma) after antigen-specific stimulation with brucellergene, which is a mixture of cytoplasmic proteins of rough brucella melitensis b115. following the depletion of the monocyte-macrophages from the pbmc, the enriched lymphocyte populations stimulated with brucellergene did not produce ifn-gamma. two-colour immunofluorescence staining of intracellular ifn-gamma and bovine cell s ... | 1998 | 9880107 |
| enzyme-linked immunosorbent assay for brucella antigen detection in human sera. | a sandwich enzyme-linked immunosorbent assay (elisa) with monoclonal antibody coupled to the solid phase was evaluated for the detection of brucella antigen in serum samples. under optimum conditions, 100 brucella cells/well or 10(5) fg/ml of lipopolysaccharide (lps) were detected in spiked specimens. the standardised assay was performed on 1607 sera from random blood donors, 146 patients with brucellosis, 20 persons in high risk groups and 264 sera from patients with diseases other than brucell ... | 1998 | 9879960 |
| risk factors for brucellosis seropositivity of goat herds in the mexicali valley of baja california, mexico. | a case-control study was conducted in the mexicali valley to identify risk factors for goat-herd seropositivity for brucella melitensis. nineteen case herds (> or = 2 positive results with the 8% rose bengal plate test (rbt)) and 55 control herds (zero positive results in rbt), matched for herdsize and geographic location, were enrolled. conditional logistic regression was used to construct a multivariable model of the odds of seropositivity using variables assessed in a questionnaire administer ... | 1998 | 9879591 |
| efficacy of elisa compared to conventional tests (rbpt and cft) for the diagnosis of brucella melitensis infection in sheep. | an indirect elisa (ielisa) using a b. abortus smooth lipopolysaccharide (s-lps) as coating antigen and a polyclonal anti-sheep igg peroxydase-labeled serum as conjugate was developed. the ielisa was assessed in comparison to the food and agriculture organisation/international atomic energy agency (fao/iaea) standard bovine elisa kit (iaea kit) using an anti-bovine igg conjugate that cross-reacts with sheep antibodies, and with complement fixation test (cft) and rose bengal plate test (rbpt). the ... | 1998 | 9874104 |
| in vitro activities of six new fluoroquinolones against brucella melitensis. | we have tested the in vitro activities of eight fluoroquinolones against 160 brucella melitensis strains. the most active was sitafloxacin (mic at which 90% of the isolates are inhibited [mic90], 0.12 microg/ml). in decreasing order, the activities (mic90s) of the rest of the tested fluoroquinolones were as follows: levofloxacin, 0.5 microg/ml; ciprofloxacin, trovafloxacin, and moxifloxacin, 1 microg/ml; and ofloxacin, grepafloxacin, and gatifloxacin, 2 microg/ml. | 1999 | 9869594 |
| antibrucella activity of quinolone sparfloxacin. | background: sparfloxacin is a new fluoroquinolone with excellent in vitro activity against a variety of gram-positive and gram-negative bacteria. methods: in vitro antibacterial activity of this new drug was tested against 139 strains of brucella melitensis. these strains were isolated from 138 patients at a major tertiary care referral center in riyadh, saudi arabia. results: seven percent of the strains were inhibited by 0.06 mg per l and 99% by 0.12 mg per l of sparfloxacin. of the five other ... | 1995 | 9815397 |
| [epidemiological analysis on the rising of brucellosis prevalence in the area already under controlled]. | an epidemiological analysis was made on the outbreak of brucellosis in sui-de county, shaanxi province in 1995-1996. result showed that it was one of the most serious brucellosis outbreaks since 1986 when national brucellosis under controlled critiria had been met in the province, and the number of cases was continuously spreading and growing. the incidence was up to 84.8 per 10(5) and the prevalence rate reached 17.5 percent. patients had typical symptoms with bad conditions. most all the cases ... | 1997 | 9812474 |
| comparison of polyclonal, monoclonal and protein g peroxidase conjugates in an enzyme-linked immunosorbent assay for the diagnosis of brucella ovis in sheep. | the sensitivities of three commercial peroxidase conjugates (polyclonal anti-sheep igg, recombinant protein g and a monoclonal anti-ruminant igg1) in an enzyme-linked immunosorbent assay for brucella ovis were evaluated. the monoclonal and protein g conjugates reduced, but did not totally remove, the characteristic background reactivity observed with the sera from brucella-free sheep. the protein g conjugate provided the best sensitivity, similar to that obtained with the classical gel diffusion ... | 1998 | 9802197 |
| evaluation of lipopolysaccharides and polysaccharides of different epitopic structures in the indirect enzyme-linked immunosorbent assay for diagnosis of brucellosis in small ruminants and cattle. | brucella abortus and brucella melitensis have surface lipopolysaccharides and polysaccharides carrying b. melitensis-type (m) and b. abortus-type (a) epitopes as well as common (c) epitopes present in all smooth brucella biotypes. crude lipopolysaccharides, hydrolytic o polysaccharides, and native hapten polysaccharides of mc or ac specificity were evaluated in indirect enzyme-linked immunosorbent assays with polyclonal, monoclonal, or protein g conjugates by using sera from cattle, sheep, and g ... | 1998 | 9801329 |
| effect of p39 gene deletion in live brucella vaccine strains on residual virulence and protective activity in mice. | the 39-kilodalton protein (p39) has previously been shown to be an immunodominant protein in brucella infections. p39 gene deletion mutants of vaccine strains brucella abortus s19 and brucella melitensis rev.1 were constructed by gene replacement. this deletion did not significantly modify the residual virulence of both vaccine strains in cd-1 mice. cd-1 mice vaccinated with the parent or mutant strains were protected against a virulent challenge. mutant vaccine strains devoid of p39 could provi ... | 1998 | 9784574 |
| identification of the perosamine synthetase gene of brucella melitensis 16m and involvement of lipopolysaccharide o side chain in brucella survival in mice and in macrophages. | brucella organisms are facultative intracellular bacteria that may infect many species of animals as well as humans. the smooth lipopolysaccharide (s-lps) has been reported to be an important virulence factor of these organisms, but the genetic basis of expression of the s-lps o antigen has not yet been described. likewise, the role of the o side chain of s-lps in the survival of brucella has not been clearly defined. a mini-tn5 transposon mutant library of brucella melitensis 16m was screened b ... | 1998 | 9784561 |
| identification of brucella by ribosomal-spacer-region pcr and differentiation of brucella canis from other brucella spp. pathogenic for humans by carbohydrate profiles. | molecular and chemical characteristics often provide complementary information in the differentiation of closely related organisms. the genus brucella consists of a highly conserved group of organisms. identification of the four species pathogenic in humans (brucella melitensis, brucella abortus, brucella suis, and brucella canis) is problematic for many clinical laboratories that depend primarily on serology and phenotypic characteristics to differentiate species. pcr amplification of the 16s-2 ... | 1998 | 9774568 |
| differentiation of brucella melitensis, b. ovis and b. suis biovar 2 strains by use of membrane protein- or cytoplasmic protein-specific gene probes. | the possibility of differentiating brucella species and biovars by southern blot hybridization of agarose gel-electrophoresed hindiii-digested genomic dna with membrane protein- or cytoplasmic protein-specific gene probes was investigated on 92 reference and field strains representative of all known species and biovars. based on the rflp pattern observed, three gene probes, i.e. br25, 39ugpa and omp16 coding for membrane or cytoplasmic proteins differentiated b. melitensis, b. ovis and b. suis b ... | 1998 | 9766202 |
| management of brucella endocarditis of a prosthetic valve. | a case of chronic brucella endocarditis of a prosthetic valve is reported. the diagnosis of this infection was established by positive blood cultures and high brucella agglutination titre. the patient was successfully managed by combination of medical therapy (consisting of streptomycin, trimethoprim-sulphamethoxazole, rifampin and tetracycline) and surgery. | 1998 | 9733385 |
| paediatric neurobrucellosis: case report and literature review. | neurological complications are rare in childhood brucellosis: there are only 33 reported cases. in children, neurobrucellosis is usually of acute presentation involving the central nervous system. we report our experience with an 8-year-old boy with brucella meningitis who demonstrated a jarisch-herxheimer-like reaction, i.e. initial clinical deterioration following the commencement of antibrucella treatment, associated with increased pleocytosis and shift from lymphocytic to polymorphic predomi ... | 1998 | 9733381 |
| non-simultaneous brucella peritonitis and meningitis in a patient with a ventriculoperitoneal shunt. | 1998 | 9721968 | |
| molecular cloning and characterization of cgs, the brucella abortus cyclic beta(1-2) glucan synthetase gene: genetic complementation of rhizobium meliloti ndvb and agrobacterium tumefaciens chvb mutants. | the animal pathogen brucella abortus contains a gene, cgs, that complemented a rhizobium meliloti nodule development (ndvb) mutant and an agrobacterium tumefaciens chromosomal virulence (chvb) mutant. the complemented strains recovered the synthesis of cyclic beta(1-2) glucan, motility, virulence in a. tumefaciens, and nitrogen fixation in r. meliloti; all traits were strictly associated with the presence of an active cyclic beta(1-2) glucan synthetase protein in the membranes. nucleotide sequen ... | 1998 | 9721274 |
| evaluation of a rough mutant of brucella melitensis in pregnant goats. | brucella melitensis strain vtrm1, a rough derivative of b melitensis strain 16m, is able to colonise the lymph nodes of goats, does not induce abortion in pregnant goats when used at doses leading to abortions with virulent strain 16m, and does not induce anti-o chain antibodies. however, strain vtrm1 as a single dose vaccine induces only partial protection against both infection and abortion following challenge. | 1998 | 9690613 |
| brucella melitensis infection in sheep: present and future. | sheep brucellosis, a zoonosis mainly due to b. melitensis (biovar 1, 2 or 3), remains widespread world-wide. pathologically and epidemiologically, the disease is very similar to b. abortus infection in cattle. the live b. melitensis rev 1 strain is currently considered as the best vaccine available for the control of sheep brucellosis, especially when used at the standard dose by the conjunctival route. used exhaustively in whole-flock vaccination programmes, it induces a great decrease in the p ... | 1998 | 9689741 |
| central nervous system brucellosis: presentation, diagnosis and treatment. | although neurological symptoms in brucellosis are frequent, central nervous system (cns) involvement is uncommon. five patients with neurobrucellosis are presented. three patients presented with meningoencephalitis, one with polyradiculoneuritis and one with myelitis and an eighth nerve palsy. all patients had lymphocytic pleocytosis, low glucose and elevated levels of protein in the cerebrospinal fluid (csf). gamma-globulin and igg values in the csf were also increased. results of agglutination ... | 1998 | 9661940 |
| diarrhoea as the first manifestation of brucellosis. | 1998 | 9629985 | |
| evaluation of north american antibody detection tests for diagnosis of brucellosis in goats. | the sensitivities and specificities of 17 antibody detection tests for brucellosis in goats were estimated. tests evaluated included the u.s. department of agriculture (usda) card test with 8% cell concentration (8%card), usda rapid automated presumptive test (rap), mexican rose bengal plate tests with 8 and 3% cell concentrations (8%rb and 3%rb), french rose bengal plate test with 4.5% cell concentration (4.5%rb), usda standard plate test (spt), usda buffered acidified plate agglutination test ... | 1998 | 9620406 |
| identification, cloning and initial characterisation of feupq in brucella suis: a new sub-family of two-component regulatory systems. | to cause disease, brucella species have to adapt to a range of different environments. environmental sensing and adaptive responses in bacteria often involve the concerted action of a two-component regulatory system, consisting of sensor and response regulator components. amplification and sequence analysis of response regulators from brucella species identified a response regulator sequence with 96% similarity to rhizobium leguminosarum feup. in r. leguminosarum, the feupq two-component system ... | 1998 | 9595675 |
| isolation of brucella melitensis from alpine ibex. | eleven alpine ibex (capra ibex) and 27 chamois (rupicapra rupicapra) from gran paradiso national park (italy) were examined in march 1996. a 7-yr-old ibex buck had thick-walled carpal joints and enlargement of the right testicle characterized by necrosis and fibrosis. microscopically, testicular lesions were characterized by large areas of necrosis, fibrosis with irregular aggregates of macrophages and lymphocytes, and scattered foci of suppuration. specimens of the carpal bursae and testicle we ... | 1998 | 9577795 |
| [43-year-old patient with undulating fever of unknown origin and abdominal symptoms]. | 1998 | 9561451 | |
| brucella-infected ovarian dermoid cyst causing initial treatment failure in a patient with acute brucellosis. | a 37-year-old woman was diagnosed with acute brucellosis. despite appropriate antimicrobial therapy, high fever persisted. a contrast enhanced ct scan of the abdomen and pelvis revealed a left ovarian dermoid cyst. on the first postoperative day after left ovariectomy and right ovarian cystectomy were performed, the patient defervesced abruptly and continued to be completely afebrile. cultures from bone marrow and dermoid cyst grew brucella melitensis. the observation of a brucella-infected ovar ... | 1998 | 9561386 |
| characterization of an immuno-dominant antigen in brucella ovis and evaluation of its use in an enzyme-linked immunosorbent assay. | a panel of 45 brucella ovis serologically positive sera were tested in immunoblots against b. ovis outer membrane proteins omp31 and omp25, purified by preparative sds-gel electrophoresis. forty-three sera reacted with omp31, while only 11 reacted with omp25, suggesting that omp31 is identical to the previously reported immuno-dominant 29-kda protein. attempts to purify omp31 on a larger scale by using procedures such as ion exchange-, reversed phase-, affinity- and gel filtration chromatography ... | 1998 | 9549861 |
| [the transmission of brucellosis via breast feeding. a report of 2 cases]. | 1998 | 9542227 | |
| human infection by brucella melitensis: an outbreak attributed to contact with infected goats. | although several outbreaks of brucella melitensis infection have been reported among laboratory workers or goat cheese consumers, outbreaks related to rural labour have been rarely studied. an outbreak of human brucellosis among farm workers of argentina was studied and revealed a close relationship with an epidemic of caprine abortions which occurred shortly before on the same farm. high rates of b. melitensis infection were found among goats. active brucellosis was diagnosed in 33 subjects (14 ... | 1997 | 9537757 |
| prostatitis and hepatitis due to brucella melitensis: a case report. | a case is reported of a 43-year-old man who presented prostatitis and hepatitis due to brucella melitensis. his symptoms were icterus, weakness, anorexia, fever, and urinary discomfort. physical examination revealed icterus and hepatosplenomegaly. lymphomonocytosis, elevated erythrocyte sedimentation rate and abnormal liver functions had been detected in laboratory tests. brucella melitensis was isolated from prostatic fluid and blood cultures. | 1998 | 9515679 |
| early clinical response to different therapeutic regimens for human brucellosis. | seventy-three cases of acute brucellosis were studied in relation to fever duration and hospital stay following different drug combinations, including gentamicin plus cotrimoxazole, rifampicin plus doxycycline, rifampicin plus cotrimoxazole, rifampicin plus tetracycline, streptomycin plus doxycycline, doxycycline plus cotrimoxazole, tetracycline plus cotrimoxazole, and tetracycline plus streptomycin. no statistical significant difference was found between these combinations regarding early clini ... | 1998 | 9502602 |
| [aneurysm of the thoracic aorta and spondylodiscitis disclosing brucellosis]. | brucellosis, which decreased during the eighties in france, remains a public health problem in many mediterranean countries. we report the case of a 65-year old patient native of morocco, of thoracic aorta aneurysm and lumbar spondylodiscitis due to brucella melitensis, revealed by haemoptysis and lumbar pains, with a favourable outcome after aortic graft resection, spinal plaster immobilization and specific lengthy antibiotic treatment. this case report is characterized by the absence of endoca ... | 1997 | 9499988 |
| evaluation of immunogenicity and protective activity in balb/c mice of the 25-kda major outer-membrane protein of brucella melitensis (omp25) expressed in escherichia coli. | the antibody response specific to the 25-kda major outer-membrane protein (omp25) of brucella melitensis expressed in escherichia coli was assessed in balb/c mice. groups of mice were immunised and boosted either with sonicated e. coli carrying plasmid pac2533-e. coli (pac2533)-expressing the gene coding for omp25 (omp25 gene) of b. melitensis, or with e. coli carrying plasmid puc19-e. coli (puc19). one control group received saline. the evolution of antibody responses was investigated by indire ... | 1998 | 9449948 |
| the characterisation of brucella strains isolated from marine mammals. | small gram negative coccobacilli isolated from seals, porpoises, dolphins and from an otter road casualty were identified as brucellae by their colonial and cell morphology, staining characteristics, biochemical activity, agglutination by monospecific antisera and susceptibility to lysis by brucella specific bacteriophage. their characterisation, including metabolic profiles, is described. these strains could not be assigned to recognised nomen species of the genus brucella and it is suggested t ... | 1997 | 9444074 |
| [spontaneous bacterial peritonitis caused by brucella]. | 1997 | 9441188 | |
| a brucella melitensis high-temperature-requirement a (htra) deletion mutant is attenuated in goats and protects against abortion. | it has been previously demonstrated that a brucella melitensis high-temperature-requirement a (htra) deletion mutant is more susceptible to oxidative killing in vitro than the parental strain and is attenuated in mice. to evaluate the contribution of the b melitensis htra protease to virulence in ruminants, the capacity of the b melitensis htra mutant rwp5 to produce abortion in goats was compared to that of the virulent parental strain 16m. experimental infection with strain 16m caused abortion ... | 1997 | 9429252 |
| detection and differentiation of the six brucella species by polymerase chain reaction. | brucelosis is a severe acute febrile disease caused by bacteria of the genus brucella. its current diagnosis is based on clinical observations that may be complemented by serology and microbiological culture tests; however, the former is limited in sensitivity and specificity, the latter is time consuming. to improve brucelosis diagnosis we developed a test which is specific and sensitive and is capable of differentiating the six species of brucella. | 1997 | 9407549 |
| a 74-year-old man with persistent fevers. | an elderly man with a history of extensive world travel presents with a chronic illness and fevers. the febrile illness has been present for eight years, and no diagnosis has been made despite extensive evaluation and testing. the differential diagnosis of this unusual case of fever of unknown origin is discussed. | 1997 | 9392941 |
| the differentiation of brucella species by substrate specific tetrazolium reduction. | in the development of a tetrazolium reduction assay to replace substrate stimulated oxygen uptake for the identification of brucella species, nine tetrazolium salts were evaluated. only the more readily reduced compounds (mtt and int) detected increased metabolic activity with the more fastidious, slow growing strains and with b. suis strains on l-arginine and dl-ornithine. the assay was optimised with mtt. mtt reduction profiles offered with the medium on which the cells were grown. cells grown ... | 1997 | 9355260 |
| brucellar osteomyelitis involving prosthetic extra-articular hardware. | we report the case of a patient with brucella melitensis osteomyelitis involving non-joint prosthetic implant of the femur. this is the first case published of osteomyelitis by brucella sp. in a patient with prosthetic implant in bone and the second one with both intra- or extra-articular prosthetic bone implant. brucella melitensis is a rare organism which causes osteomyelitis in patients with prosthetic hardware, and should be added to the list of suspected organisms responsible for this disea ... | 1997 | 9354359 |
| [fever and dry cough in a construction worker from portugal]. | a 33-year-old portugese worker presented with a one-week history of nonproductive cough and fever. a presumptive diagnosis "viral infection of the respiratory tract" was made. however, because of persisting cough and fever further investigations were necessary, and finally brucella melitensis was isolated in blood cultures. three months before admission to the hospital the man was dressing the carcasses of a goat in portugal and consumpted fresh goats milk cheese. antibiotic therapy with rifampi ... | 1997 | 9340710 |
| rapid detection of brucella melitensis from blood cultures by a commercial system. | to assess the capability of the peds plus medium of the bactec 9240 blood culture system to recover brucella melitensis within the routine seven-day protocol used by most clinical microbiology laboratories, inoculated blood culture bottles were monitored by the bactec 9240 instrument for four weeks, and blind subcultures were performed once a week. a total of 2579 blood cultures were drawn, 42 (1.6%) of which were positive for brucella melitensis. forty-one of the 42 (97.6%) positive cultures we ... | 1997 | 9323475 |
| scrotal gray-scale and color doppler sonographic findings in genitourinary brucellosis. | brucellosis is an enzootic disease that is frequently transmitted from brucella-infected animals and their products to humans through the gastrointestinal tract. genitourinary complications are seen in 2-10% of cases. we used sonography to investigate such complications in patients from a brucella-endemic area. | 1997 | 9321717 |
| survival of a bacterioferritin deletion mutant of brucella melitensis 16m in human monocyte-derived macrophages. | a bacterioferritin (bfr) deletion mutant of brucella melitensis 16m was generated by gene replacement. the deletion was complemented with a broad-host-range vector carrying the wild-type bfr gene, pbbr-bfr. the survival and growth of the mutant, b. melitensis pad 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (mdm). these results suggest that bfr is not essential for the intracellular survival of b. melitensis in human mdm. | 1997 | 9317046 |
| dna polymorphism at the omp-31 locus of brucella spp.: evidence for a large deletion in brucella abortus, and other species-specific markers. | the omp-31 gene, encoding a major outer-membrane protein in brucella melitensis, was pcr-amplified from brucella strains representing all species and known biovars by using primers selected according to the b. melitensis 16m omp-31 published sequence. amplification of omp-31 was achieved from dna of all brucella species with the exception of brucella abortus, the only brucella species where expression of omp-31 was not detected by reactivity with an mab specific for an epitope located in omp-31. ... | 1997 | 9308175 |
| [fever, pulmonary infiltrates and hepatosplenomegaly]. | 1997 | 9304141 | |
| identification and characterization of brucella ovis immunogenic proteins using two-dimensional electrophoresis and immunoblotting. | in a previous report, proteins from brucella melitensis were characterized by two-dimensional polyacrylamide gel electrophoresis (2-d page) and n-terminal microsequencing. in the present report, we have extended this study to the second etiologic agent in ovine brucellosis, b. ovis, responsible for ram epididymitis and infertility. the combination of 2-d gel electrophoresis and protein microsequencing facilitated the location and identification of the major proteins of b. ovis on the 2-d pattern ... | 1997 | 9298663 |
| localization and characterization of a specific linear epitope of the brucella dnak protein. | we have previously produced and characterized four monoclonal antibodies to the brucella dnak protein which were derived from mice infected with b. melitensis or immunized with the b. melitensis cell wall fraction. by use of a recombinant dna technique, we have localized a linear epitope, recognized by two of these monoclonal antibodies (v78/07b01/g11 and v78/09d04/d08), in the last 21 amino acids of the c-terminal region of the brucella dnak protein. the c-terminal region has been reported to b ... | 1997 | 9297829 |
| identification of the major t-cell antigens present in the brucella melitensis b115 protein preparation, brucellergene ocb. | brucellergene is a commercial allergen prepared from brucella melitensis strain b115 and containing at least 20 cytoplasmic proteins. these proteins were separated by sds-page. the unstained gel was divided into 18 fractions and proteins were eluted from the gel fractions. the capacity of the separated proteins to elicit delayed-type hypersensitivity (dth) in infected guinea-pigs or to induce the production of interferon-gamma (ifn-gamma) by blood cells from infected cattle was evaluated. the bi ... | 1997 | 9291893 |
| serological findings obtained in cattle herds immunised with the brucella melitensis rev.1 and the b. abortus b19 vaccine in mongolia. | to reduce the economic losses caused by brucella abortus infection under the conditions of nomadic cattle breeding, more than half million cattle over 3 months of age (excluding male animals) were immunised with the b. melitensis rev.1 vaccine in 7 provinces of mongolia in the first year of a programme (1987). in the second year, only heifer calves over 3 months of age, yearling animals not vaccinated in the first year, as well as heifers over 2 years of age and cows were vaccinated. the vaccine ... | 1997 | 9270127 |
| a review of the use of b. melitensis rev 1 vaccine in adult sheep and goats. | the live brucella melitensis rev 1 strain is considered the best vaccine available for the prophylaxis of brucellosis in small ruminants. the classically recommended exclusive vaccination of young replacement animals has failed to control brucellosis in some developed countries and is frequently inapplicable in the developing world. accordingly, whole-flock vaccination is the only feasible alternative to control b. melitensis infection in small ruminants under the extensive management conditions ... | 1997 | 9234451 |
| [the functional metabolic activity of the blood neutrophils in different clinical forms of brucellosis]. | the characteristics of the activity of alkaline phosphatase, nonspecific esterase, myeloperoxidase, lysosomal cation proteins, lipids have been determined in the cytochemical study of neutrophils in peripheral blood samples of 127 patients with acute, chronic, primary chronic brucellosis and osteochondrosis. it is expedient to use these characteristics as additional tests for the differential diagnostics of different clinical forms of brucellosis, as well as residual brucellosis and osteochondro ... | 1997 | 9221662 |
| [15th international symposium of salmonellosis and brucellosis: a report]. | 1997 | 9221550 | |
| infection of a pacemaker by brucella melitensis. | we report a case that, to the best of our knowledge, is the only published instance of infection of a pacemaker and its leads by brucella melitensis. furthermore, this case suggests that b. melitensis may be able to persist around pacemaker devices despite its having been eliminated from the rest of the body. the patient was a sheep shearer who had just undergone a 45-day course of antibiotic therapy for brucella and had been considered cured on the basis of negative blood cultures. | 1997 | 9205989 |
| brucellosis: an overview. | brucellosis remains a major zoonosis worldwide. although many countries have eradicated brucella abortus from cattle, in some areas brucella melitensis has emerged as a cause of infection in this species as well as in sheep and goats. despite vaccination campaigns with the rev 1 strain, b. melitensis remains the principal cause of human brucellosis. brucella suis is also emerging as an agent of infection in cattle, thus extending its opportunities to infect humans. the recent isolation of distin ... | 1997 | 9204307 |
| the economic impact of a bioterrorist attack: are prevention and postattack intervention programs justifiable? | understanding and quantifying the impact of a bioterrorist attack are essential in developing public health preparedness for such an attack. we constructed a model that compares the impact of three classic agents of biologic warfare (bacillus anthracis, brucella melitensis, and francisella tularensis) when released as aerosols in the suburb of a major city. the model shows that the economic impact of a bioterrorist attack can range from an estimated $477.7 million per 100,000 persons exposed (br ... | 1997 | 9204289 |
| transmission of brucella melitensis from sheep to lambs. | forty-one pregnant sheep showing positive immune responses to brucella melitensis in serological or allergic tests were selected from naturally infected flocks and kept in an isolated pen for lambing. the resulting 62 lambs were maintained in the same pen with their dams during lactation. when the lambs were weaned, the dams were slaughtered for bacteriological study and the lambs were reared in a clean pen. fourteen ewes excreted b melitensis during lactation and 17 were found to be infected po ... | 1997 | 9203318 |
| lymphocyte proliferative responses of goats vaccinated with brucella melitensis 16m or a delta pure201 strain. | the response to a brucella melitensis purek deletion mutant, delta pure201 (referred to as strain 201), was compared with the response to its parental strain, 16m, in juvenile goats. proliferative responses to gamma-irradiated bacteria were detected earlier in strain 201-infected goats. lymphocytes from strain 16m- or 201-infected goats proliferated in response to one-dimensional polyacrylamide gel electrophoresis-separated proteins of similar mass isolated from strain 16m or brucella abortus rb ... | 1997 | 9199478 |
| serological serum protein fraction and responses to brucella melitensis in lambs fed aflatoxins. | twenty-four lambs were given 2 ppm aflatoxins (af) in their diet for 37 d and 12 were kept as a control group. after this time, toxic feed was removed for a further 35 days (clearance period). on day 17 all lambs were vaccinated with b melitensis strain rev-1, and blood samples were taken regularly to determine the levels of antibodies and serum proteins. aflatoxins decreased titers of rose bengal test and optical densities of elisa, both in the intoxicated and clearance periods. complement fixa ... | 1997 | 9167241 |
| comparison of bactec 9240 peds plus medium and isolator 1.5 microbial tube for detection of brucella melitensis from blood cultures. | the sensitivity and time to detection of brucella melitensis by the bactec 9240 and the isolator blood culture systems were compared in a prospective volume-controlled study. blood sample aliquots, obtained from children with suspected brucellosis, were inoculated into a bactec 9240 peds plus bottle and into an isolator 1.5 microbial tube. overall, 122 pairs of blood samples for culture were obtained, and 28 (23%) were positive by at least one method. the bactec 9240 system detected all 28 posit ... | 1997 | 9163448 |
| a clinical study of brucellosis in adults in the asir region of southern saudi arabia. | one hundred four saudi patients with brucellosis who were admitted to abha general hospital in the asir region of southern saudi arabia were studied. all the patients had brucella melitensis infection; 61.5% were females while 38.5% were males. their mean age was 32 years. most of the patients (61.5%) lived in the lowland (tihama) and the majority were shepherds (84.6%). the most common presenting symptoms were fever (100%), sweating (96.2%), headache (76.9%), joint pains (76.9%), and backache ( ... | 1997 | 9158043 |
| antibody and delayed-type hypersensitivity responses to ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough brucella spp. | immunological cross-reactions between brucella spp. and ochrobactrum anthropi were investigated in animals and humans naturally infected by brucella spp. and in experimentally infected rams (brucella ovis infected), rabbits (brucella melitensis infected), and mice (b. melitensis and brucella abortus infected). in the animals tested, o. anthropi cytosolic proteins evoked a delayed-type hypersensitivity reaction of a frequency and intensity similar to that observed with b. melitensis brucellin. o. ... | 1997 | 9144364 |
| brucella melitensis osteitis following craniotomy in a patient with aids. | 1997 | 9142817 | |
| characterization of smooth lipopolysaccharides and o polysaccharides of brucella species by competition binding assays with monoclonal antibodies. | previously, four epitope specificities on the o chain of brucella species were reported: m, a, c, and c/y. in this work, according to monoclonal antibody binding to smooth lipopolysaccharides of yersinia enterocolitica 0:9, brucella abortus w99 (a-dominant strain), and b. melitensis rev1 (m-dominant strain), seven o-chain epitope specificities were defined: m, a, c (m > a), c (m = a), c/y (m > a), c/y (m = a) and c/y (a > m). competitive binding assays between these monoclonal antibodies suggest ... | 1997 | 9125584 |
| brucellosis in fish. | brucellosis is a zoonotic infectious disease that represents serious economic and health problems. it has been extensively studied in different species of livestock but literary references to fish brucellosis are scarce. therefore, the purpose of the research hereby presented was to investigate the serologic-bacteriologic response of nile catfish to experimental infection with brucellosis. thirty predatory catfishes were used. they were divided into two groups. the first was subjected to s. c. i ... | 1997 | 9123781 |
| total knee arthroplasties infected by brucella melitensis: a case report. | a 74-year-old man with bilateral knee arthroplasties developed brucella arthritis in both joints after a total joint arthroplasty in his left knee. this complication is the second case reported in the literature to date. | 1997 | 9113296 |
| mapping and identification of brucella melitensis proteins by two-dimensional electrophoresis and microsequencing. | two-dimensional (2-d) gel electrophoresis was used to map brucella melitensis proteins. the 2-d proteins map of b. melitensis b115 revealed 595 silver-stained protein spots separated by both isoelectric point and molecular mass. twenty-five proteins were identified either by immunoblotting using monoclonal antibodies (mabs) or by n-terminal microsequencing. the protein spots identified by mabs were the 89 kda outer membrane protein, dnak, bacterioferritin, cp24, and bp26. some spots were identif ... | 1997 | 9059838 |
| [infectious-allergic ophthalmic brucellosis (the validation of a new nosological form)]. | the necessity of distinguishing a new nosological entity: infectious allergic ophthalmic brucellosis-is validated. based on her own clinical and experimental findings and published data, the author describes the etiology, pathogenesis, clinical picture, diagnosis, and treatment of this disease. | 1996 | 9045486 |
| production and characterization of monoclonal antibodies specific for the lipopolysaccharide of escherichia coli o157. | identification of the o157 antigen is an essential part of the detection of escherichia coli o157:h7, which is recognized as a major etiologic agent of hemorrhagic colitis. however, polyclonal antibodies produced against e. coli o157:h7 lipopolysaccharide (lps) may react with several other bacteria including brucella abortus, brucella melitensis, yersinia enterocolitica o9, escherichia hermannii, and stenotrophomonas maltophilia. we produced eight monoclonal antibodies (mabs) specific for the lp ... | 1997 | 9041412 |
| characterization, occurrence, and molecular cloning of a 39-kilodalton brucella abortus cytoplasmic protein immunodominant in cattle. | monoclonal antibodies and polyclonal antisera recognizing a 39-kda protein (p39) of brucellin, a cytoplasmic extract from brucella melitensis rough strain b115, were produced. the p39 was purified by anion-exchange chromatography. eleven of fourteen brucella-infected cows whose infections had been detected by the delayed-type hypersensitivity (dth) test with brucellergen also developed a dth reaction when purified p39 was used as the trigger. the t-cell proliferative responses to p39 of peripher ... | 1997 | 9009303 |
| competitive enzyme-linked immunosorbent assay using monoclonal antibodies to the brucella melitensis bp26 protein to evaluate antibody responses in infected and b. melitensis rev.1 vaccinated sheep. | competitive enzyme-linked immunosorbent assay (c-elisa) was performed using 15 monoclonal antibodies (mabs), specific for brucella bp26 (previously also called cp28), a periplasmic protein antigen, to investigate antibody responses in naturally and b. melitensis h38 experimentally infected and b. melitensis rev.1 vaccinated sheep. the antigen preparation consisted of cytosoluble protein extract (cpe) of b. melitensis b115. by combining the c-elisa results of several mabs, a high percentage of na ... | 1996 | 9008343 |
| [epidemic outbreak of 81 cases of brucellosis following the consumption of fresh cheese without pasteurization]. | in spite of the great effort that has been made in recent years in castilla-la mancha to control brucelosis, a lack of awareness on the part of producers and consumers leads to major epidemic outbreak, such as the one described below. | 1996 | 9005033 |
| congenital brucellosis in a premature infant. | 1997 | 9002111 | |
| detection of brucella melitensis by bactec nr 730 and bactec 9120 systems. | in many parts of the world, brucellosis has significantly decreased, but it is still a problem in some regions of turkey. in our laboratory we have isolated 58 brucella spp. through bactec nr 730 and 30 brucella spp. through bactec 9120 systems. 31 of the 58 isolates were detected by bactec nr 730 system in 72 hours. the majority of the growth values were between 45-65. bactec 9120 system detected all the isolates in 84 hours. | 1996 | 8982628 |
| delayed-type hypersensitivity activity of the brucella l7/l12 ribosomal protein depends on posttranslational modification. | the ribosomal protein l7/l12 isolated from brucella melitensis induces a delayed-type hypersensitivity (dth) reaction in brucella-sensitized guinea pigs. surprisingly, the recombinant brucella l7/l12 protein expressed in escherichia coli as a fusion protein with a six-histidine tag cannot elicit such a reaction. the six histidines tagged to the recombinant l7/l12 protein were removed enzymatically, but the resulting protein did not induce a dth reaction in sensitized animals. incubation of the r ... | 1997 | 8975922 |
| serological and bacteriological study of swine brucellosis. | a serological and bacteriological study was performed with sera taken from 2,228 swine from six states in venezuela. none of the animals were vaccinated against brucellosis, and the prevalence of the disease varied from 5 to 89% on farms located in these states. our studies indicated that the animals could be categorized into four groups depending on the degree of reactivity in serological tests. brucella suis biovar 1 was isolated from the lymph nodes, spleens, and semen samples of seropositive ... | 1997 | 8968931 |
| recombinant brucella abortus proteins that induce proliferation and gamma-interferon secretion by cd4+ t cells from brucella-vaccinated mice and delayed-type hypersensitivity in sensitized guinea pigs. | optimal protective immunity to brucella abortus infection is dependent on a coordinate interaction between different t-cell subsets which leads to an antigen-specific t-lymphocyte-mediated activation of macrophages, the main cellular reservoir for the bacterium. as an initial step in the identification of bacterial proteins that mediate cellular immunity, we have subcloned the b. abortus ssb, uvra, groes, and groel genes into the prokaryotic expression vector pmal-c2 using pcr. escherichia coli ... | 1996 | 8964089 |
| breast abscess caused by brucella melitensis. | cutaneous and soft tissue lesions are uncommon manifestations of brucellosis. though breast involvement in animal brucellosis is not uncommon, involvement of the breast in human brucellosis is extremely rare. we report a case of breast abscess in a 39-year-old female caused by brucella melitensis. treatment with combination of trimethoprim/sulphamethoxazole (tmp/ smx; cotrimoxazole) and doxycycline for 3 months resulted in clinical cure. | 1996 | 8945713 |
| outer membrane protein of neisseria meningitidis as a mucosal adjuvant for lipopolysaccharide of brucella melitensis in mouse and guinea pig intranasal immunization models. | a mucosal vaccine against brucellosis consisting of the lipopolysaccharide (lps) of brucella melitensis complexed with the outer membrane protein (gbomp) of group b neisseria meningitidis was tested in small-animal models of intranasal immunization. mice given two doses of the vaccine developed high levels of immunoglobulin g (igg) and iga antibodies specific for b. melitensis lps in lung lavages and specific igg and iga antibody-secreting cells in the lungs and spleen. similarly, in guinea pigs ... | 1996 | 8945575 |
| brucella infection of the thyroid gland. | three cases of brucella infection of the thyroid gland are reported. all three were female, and two were from rural areas in tehran province. all presented with fever, chills, and painful swelling in the neck, had positive wright and 2-mercaptoethanol test; and had normal thyroid function. cultures of fna materials grew brucella in all cases. they were cured following antibiotic treatment. | 1996 | 8936672 |
| significance of environmental conditions (ph and serum) on the in vitro potency of azithromycin against brucella melitensis. | 1995 | 8904096 | |
| cloning, nucleotide sequence, and expression of the gene coding for a ribosome releasing factor-homologous protein of brucella melitensis. | the gene coding for a brucella melitensis cytosoluble protein (cp24) that is immunogenic in infected sheep and a major component of brucellin inra was cloned and sequenced. as in brucella cells, cp24 was located in the cytoplasm of recombinant escherichia coli. the amino acid sequence predicted from the cloned gene revealed 48 and 46% identity with the ribosome releasing factor, a protein factor required for release of the 70s ribosome from the mrna, of e. coli and haemophilus influenzae rd, res ... | 1996 | 8890247 |
| surgical treatment of brucella endocarditis. | between april 1987 and october 1992, six cases of brucella endocarditis were operated on in the authors' hospital for valve replacement. they were five men and one woman with a mean(s.d.) age of 52(15) years (range 30-71 years). three patients were in new york heart association (nyha) class iii and three in class iv. two patients had previous history of rheumatic fever, one was a drug abuser, two had peripheral embolism and one constrictive pericarditis. most were living in rural areas. echocard ... | 1996 | 8861443 |
| brucella melitensis biotype 1 outbreak in goats in northern kwazulu-natal. | brucella melitensis biotype 1 was confirmed in indigenous, outbred goats in three northern districts of the kwazulu-natal province following the diagnosis of human malta fever in the same area. six foci of infection were found during an extensive serological survey involving 6266 goats carried out in most of the districts of the kwazulu-natal province. the prevalence in the positive herds varied between 17% and 100%. the diagnosis was confirmed by culturing milk samples from serologically positi ... | 1996 | 8856768 |
| hepatosplenic abscesses due to brucella melitensis: report of a case involving a child and review of the literature. | suppurative disease of the liver and/or spleen is a rare and serious complication of human brucellosis. in the english-language literature, only nine cases have been reported, all involving adults with chronic infection. we report the case of a young child in whom abscesses of the liver and spleen developed during acute brucellosis. brucella melitensis was cultured from an aspirate of the liver and from the bone marrow. after percutaneous drainage of the liver abscess, the patient responded to a ... | 1996 | 8852967 |
| serological follow-up of human brucellosis by measuring igg antibodies to lipopolysaccharide and cytoplasmic proteins of brucella species. | a serological 2-year follow-up of 35 patients who had brucellosis with different clinical outcomes was performed. patients were followed up by standard tube agglutination (sta) and 2-mercaptoethanol sta (2me-sta) tests and by two enzyme-linked immunosorbent assays (elisas) to independently measure values of serum igg to either lipopolysaccharide (lps) or cytoplasmic proteins of brucella. whereas sta and 2me-sta tests revealed characteristic antibody profiles for patients who recovered (group i), ... | 1996 | 8852961 |
| chronic subdural empyema: a new presentation of neurobrucellosis. | 1996 | 8842288 | |
| inadequate azithromycin activity against brucella melitensis in mice with acute or chronic infections. | the activities of therapeutic regimens with azithromycin (azi) and doxycycline combined with streptomycin (dox-sm) were compared in brucella melitensis infected mice. in a chronic model, azi given over 10, 14 or 21 consecutive days (50 mg/kg/24 h) significantly reduced the infection (1.3-1.6 logs, day 48 post-infection). however, the effectiveness of dox (21 days, 50 mg/kg/12 h) was higher than azi (3.4 logs of reduction, day 48 post-infection). besides, when dox was administered for 45 days, it ... | 1996 | 8835110 |
| first report of a strain of brucella melitensis that was widely sensitive to brucellaphages isolated in the united arab emirates. | 1996 | 8825005 |