Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| responses of adipose and muscle lipoprotein lipase to chronic infection and subsequent acute lipopolysaccharide challenge. | infection of male swiss webster mice with toxoplasma gondii or neospora caninum leads to long-term alterations in energy balance. following an initial 20 to 30% weight loss in all t. gondii-infected mice, half of the animals regain most of the lost weight (gainers), whereas the others maintain their low body weight (nongainers). infection with n. caninum does not elicit weight loss. lipoprotein lipase (lpl), the enzyme responsible for plasma triglyceride (tg) clearance and partitioning among tis ... | 2002 | 12093671 |
| coprodiagnosis of hammondia heydorni in dogs by pcr based amplification of its 1 rrna: differentiation from morphologically indistinguishable oocysts of neospora caninum. | hammondia heydorni is thought to be a non-pathogenic coccidian parasite of dogs that is closely related to neospora caninum, an important parasite of cattle and dogs. oocysts of these two species are morphologically indistinguishable from each other. a population of 2240 dogs in the czech republic was screened for the presence of h. heydorni/n. caninum oocysts and five (0.22%), represented by five of 3135 faecal samples (0.16%), were positive. the internal transcribed spacer 1 region of the rrna ... | 2002 | 12093189 |
| neosporosis in a white rhinoceros (ceratotherium simum) calf. | a 16-day-old white rhinoceros calf died suddenly while in excellent condition and showing no obvious previous clinical signs. it was the 9th calf of a mature female kept free-ranging with 11 other rhinoceros as well as various other game species on a 2000 hectare game breeding centre adjacent to the town of lichtenburg and outlying cattle farmlands in the northern province. at post mortem examination, death was ascribed to heart failure. there was marked multifocal to coalescing subacute parasit ... | 2002 | 12088073 |
| serological survey of neospora caninum and leishmania infantum co-infection in dogs. | a seroprevalence survey and risk analysis of neospora caninum and leishmania infantum was conducted in dogs from an area of the campania region of southern italy, in order to investigate the co-infection of these two protozoa. blood samples were collected from 1058 asymptomatic dogs over a 18 months period. serum samples were tested for antibodies to n. caninum and to l. infantum using the indirect fluorescent antibody test. epidemiological data (breed, age, sex, and utilization) were collected ... | 2002 | 12079736 |
| p38-avidity-elisa: examination of herds experiencing epidemic or endemic neospora caninum-associated bovine abortion. | an enzyme-linked immunosorbent assay (elisa) was established to measure the avidity of bovine igg directed against p38, a surface antigen (ncsrs2) of neospora caninum tachyzoites. in the sera of dams intravenously infected with n. caninum nc-1, the p38-specific avidity increased from initially below 40% up to avidity indices between 50 and 80% after days 23 and 91 p.i. the p38-avidity-elisa was used to examine various herds that had experienced endemic and epidemic n. caninum-associated bovine a ... | 2002 | 12079735 |
| redescription of neospora caninum and its differentiation from related coccidia. | neospora caninum is a protozoan parasite of animals, which before 1984 was misidentified as toxoplasma gondii. infection by this parasite is a major cause of abortion in cattle and causes paralysis in dogs. since the original description of n. caninum in 1988, considerable progress has been made in the understanding of its life cycle, biology, genetics and diagnosis. in this article, the authors redescribe the parasite, distinguish it from related coccidia, and provide accession numbers to its t ... | 2002 | 12076623 |
| pattern of recognition of neospora caninum tachyzoite antigens by naturally infected pregnant cattle and aborted foetuses. | different aspects of neospora tachyzoite antigen recognition by neospora-infected heifers and cows and aborted foetuses were studied. the pattern of antigen recognition and the relationship between ifat titres and number of neospora antigens detected, were evaluated. in addition, the tachyzoite antigens involved in the humoral immune response developed against infection in normal cows and cows that aborted were also characterised throughout pregnancy. comparison of tachyzoite antigen recognition ... | 2002 | 12072210 |
| immunisation of mice against neosporosis. | in the present study a murine encephalitis model was used to investigate if protection against neosporosis could be achieved by immunisation. groups of 10 mice were immunised with a sublethal dose of live neospora caninum tachyzoites, n. caninum antigens incorporated into iscoms, n. caninum lysate mixed with quil a, or n. caninum lysate in pbs. control mice were given quil a only. challenge infection with 2.5x10(6) n. caninum tachyzoites resulted in clinical symptoms that remained until the end ... | 2002 | 12062558 |
| a longitudinal study of neospora caninum infection on a dairy farm in new zealand. | a 600-cow new zealand dairy herd experienced an abortion storm in 1997 and was monitored (blood sampling at about 3-month intervals) from may 1997 until january 1999. abortion risk reached 9% in 1997 and was highest in heifers at 19%. the abortion risk decreased in 1998 to 3.2% (still somewhat higher than during the years prior to the outbreak). the serological reaction pattern for neospora caninum showed an association with abortion risk only around the time of the 1997 outbreak when seropositi ... | 2002 | 12062516 |
| vero cell surface proteoglycan interaction with the microneme protein ncmic(3) mediates adhesion of neospora caninum tachyzoites to host cells unlike that in toxoplasma gondii. | neospora caninum and toxoplasma gondii are characterised by a very low host cell specificity, thus they are able to infect a wide range of different cells in vivo and in vitro. infection of the host cell by tachyzoites is a process which is preceded by adhesion onto the host cell surface. the receptors on the host cell surface which would allow n. caninum to establish a physical interaction have not been investigated so far. here we report the role of host cell surface proteoglycans as receptors ... | 2002 | 12062488 |
| prevalence of antibodies to neospora caninum in white-tailed deer, odocoileus virginianus, from the southeastern united states. | serum samples from 305 white-tailed deer (odocoileus virginianus) from 14 states in the southeastern united states were examined for antibodies to neospora caninum using a direct agglutination test. positive agglutination titers were found in 145 (48%) of the white-tailed deer examined: 21 (7%) had titers of 1:25, 92 (30%) had titers of 1:50, and 32 (10%) had titers of > or = 1:500. these findings that antibodies to n. caninum are common in white-tailed deer support the concept that a sylvatic c ... | 2002 | 12054027 |
| prevalence of neospora caninum antibodies in dogs from dairy cattle farms in parana, brazil. | serum samples from 134 dogs from 22 cattle dairy farms in the northern region of parana state, brazil, were tested for antibodies to neospora caninum in an indirect fluorescent antibody test. antibodies (> or = 1:50) to n. caninum were found in 29 (21.6%) of the 134 dogs, and seropositive dogs were found on 14 (63.6%) of the 22 dairy cattle farrms. the antibody titers of dogs were 1:50 (3 dogs), 1:100 (7 dogs), 1:200 (7 dogs), 1:400 (6 dogs), and > or = 1:800 (6 dogs). the low prevalence (9%) in ... | 2002 | 12054023 |
| susceptibility of psammomys obesus and meriones tristrami to tachyzoites of neospora caninum. | the susceptibility of psammomys obesus (sand rat) and meriones tristrami (tristram's jird) to neospora caninum was investigated by subcutaneous (s.c.) and intraperitoneal (i.p.) inoculation of 10-fold doses of culture-derived tachyzoites. groups of 5 animals were inoculated with doses of 10-10(7) parasites via each route of inoculation. all but 2 of the sand rats inoculated with doses of 10-10(4) parasites succumbed to the infection by 7-18 days postinfection. all jirds inoculated with 10(7) tac ... | 2002 | 12054004 |
| immune factors influencing the course of infection with neospora caninum in the murine host. | this paper investigates the role of specific immune factors on the course of infection in genetic knockout (gko) mice infected with 3 different strains of neospora caninum. survival time and parasite persistence were examined in interferon-gamma (ifn-gamma), tumor necrosis factor receptor-2 (tnfr2), interleukin 10 (il-10), beta 2 microglobulin (beta2m), and inducible nitric oxide synthase (inos2) gko or wild-type (wt) mice following infection with either pathogenic (nc-1 or nc-2) or attenuated ( ... | 2002 | 12053997 |
| hepatic sarcocystosis in a striped dolphin (stenella coeruleoalba) from the spanish mediterranean coast. | fatal hepatic sarcocystosis was diagnosed in a striped dolphin (stenella coeruleoalba) from the northeastern spanish mediterranean coast based on pathologic findings and the microscopic and ultrastructural characteristics of the intralesional parasite. main gross lesions were icterus, subcutaneous hemorrhages, and hepatic congestion. the most prominent microscopic lesions consisted of severe acute multifocal to coalescing necrotizing hepatitis with cholestasis and intralesional protozoa. there w ... | 2002 | 12053971 |
| occurrence of neospora caninum antibodies in sera from dogs of the city of são paulo, brazil. | neospora caninum is an important cause of abortion in dairy cattle worldwide. dogs are important in the epidemiology of this parasite because they are the only hosts known to excrete n. caninum oocysts. in order to understand the prevalence of n. caninum in dogs, sera from 500 owned dogs and from over 600 feral street dogs from the city of são paulo, brazil were assayed for antibodies to n. caninum. sera were examined by the neospora agglutination test (nat) using mouse-derived tachyzoites. anti ... | 2002 | 12031819 |
| serological survey of neospora caninum infection in dairy cattle herds in aguascalientes, mexico. | a serological survey for antibody activity to neospora caninum was carried out in aguascalientes, a state in the central part of mexico. one-hundred and eighty-seven serum samples from 13 dairy herds were tested by the elisa test. the herd prevalence was 100% and the overall prevalence was 59% (n=110). seventy-six of 97 seropositive cows had previous records of abortion. there was a statistically significant difference between the groups (p<0.05). however, the odds ratio was 1.4, suggesting an a ... | 2002 | 12031815 |
| trigeminal neuropathy in dogs: a retrospective study of 29 cases (1991-2000). | the medical records of 29 dogs unable to close their mouths due to flaccid paralysis or paresis of the muscles innervated by the mandibular branch of the trigeminal nerve, were reviewed. idiopathic trigeminal neuropathy was diagnosed in 26 dogs based on complete resolution of clinical signs and lack of any long-term neurological disease. of these dogs, golden retrievers were overrepresented. no age, sex, or seasonal predispositions were identified. trigeminal sensory innervation deficits were ob ... | 2002 | 12022413 |
| epidemiological investigations of abortions due to neospora caninum on swiss dairy farms. | abortions apparently due to neospora caninum in two swiss dairy herds were investigated by means of a pcr and elisa, and other potential causes were eliminated. in addition, a case-control study of 24 case herds and 24 control herds indicated that n caninum-associated abortions were more likely to occur in herds with antibodies to coxiella burnetii (with an odds ratio [or] of 3.38 with a 95 per cent confidence interval [ci] of 1.82 to 6.22), whereas the likelihood was less in herds with antibodi ... | 2002 | 12019533 |
| identification of a neospora caninum microneme protein (ncmic1) which interacts with sulfated host cell surface glycosaminoglycans. | the invasive stages of apicomplexan parasites enter their host cells through mechanisms which are largely conserved throughout the phylum. host cell invasion is divided into two distinct events, namely, adhesion onto the host cell surface and the actual host cell entry process. the former is mediated largely through microneme proteins which are secreted at the onset of establishing contact with the host cell surface. many of the microneme proteins identified so far contain adhesive domains. we h ... | 2002 | 12011014 |
| immunological relationship between neospora caninum and besnoitia besnoiti. | neospora caninum is a coccidian parasite identified as a major cause of abortion in cattle. a combined infection of n. caninum with another taxonomically related parasite of cattle, besnoitia besnoiti can occur in geographical areas endemic for both species. both infections are routinely diagnosed serologically, and incorrect diagnosis could occur if immunological cross-reactivity exists between the two parasites. to investigate the possible degree of cross-reactivity, we compared results obtain ... | 2002 | 11992709 |
| sensitive and specific identification of neospora caninum infection of cattle based on detection of serum antibodies to recombinant ncp29. | neosporosis is an economically important disease of dairy cattle caused by the protozoan neospora caninum. diagnostic tests for neosporosis are complicated by the potential for cross-reaction of antibodies to antigens that are similar between n. caninum and closely related parasites toxoplasma gondii and sarcocystis cruzi. to provide a sensitive and specific assay for detecting antibodies to n. caninum in the serum of infected animals, we have investigated a recombinant form of the antigen known ... | 2002 | 11986268 |
| use of a serum-free medium to produce in vitro neospora caninum and toxoplasma gondii tachyzoites on vero cells. | neospora caninum and toxoplasma gondii are cyst-forming coccidian parasites of human and veterinary clinical relevance. in vitro cultivation of the protozoans using vero cells is usually performed in order to produce antigenic materials. quantitative and qualitative comparisons of vero cells grown in rpmi medium supplemented either with foetal calf serum (fcs), horse serum (hs) or a specific serum-free additive (defcell) were performed. a serum-free cell culture system used to propagate n. canin ... | 2002 | 11944805 |
| diagnosis and seroepidemiology of neospora caninum-associated bovine abortion. | a round table was conducted at the viiith international coccidiosis conference on neospora diagnosis with particular emphasis on strategies to diagnose bovine abortion. the strength and weakness of different assays for neospora caninum infection and whether these methods have resulted in the overdiagnosis of neosporosis was discussed. it was evident that each diagnostic method, namely histology, immunohistochemistry, molecular detection and serological assays were, under certain circumstances, v ... | 2002 | 11943234 |
| companion animal parasitology: a clinical perspective. | in recent years there have been many changes to the ways that clinical veterinary science is conducted and nowhere is this more evident than in companion animal practice. veterinarians working with pet dogs and cats are facing new challenges associated with the emergence and re-emergence of parasitic diseases. some, such as neospora caninum, have been recently recognised; others like giardia and cryptosporidium have been reported with increasing frequency, in part as a result of laboratory tests ... | 2002 | 11943231 |
| infection of organotypic slice cultures from rat central nervous tissue with neospora caninum: an alternative approach to study host-parasite interactions. | neospora caninum is an apicomplexan parasite which has emerged as an important cause of bovine abortion worldwide. abortion is usually triggered by reactivation of dormant bradyzoites during pregnancy and subsequent congenital infection of the foetus, where the central nervous system appears to be most frequently affected. we here report on an organotypic tissue culture model for neospora infection which can be used to study certain aspects of the cerebral phase of neosporosis within the context ... | 2002 | 11943226 |
| factors associated with neospora caninum serostatus in cattle of 20 specialised costa rican dairy herds. | twenty-five specialised costa rican dairy farms (located in the poás area) were used to determine neosporosis seroprevalence and the association of seropositivity with environmental and management factors. the farms involved were selected intentionally and all of them use vampp 5.1 (veterinary automated management and production control programme) as management-information system. holstein-friesian, jersey and crosses between them were the most-frequent breeds in these herds. the number of femal ... | 2002 | 11937233 |
| neospora caninum is an invalid species name: an evaluation of facts and statements. | an evaluation of both the formal requirements of the international rules of zoological nomenclature and the scientific reasons for the description of new genera and species shows that the name neospora caninum is a nomen nudum. the only characteristic criteria for discriminating between the previously described species hammondia heydorni and the proposed new species n. caninum (i.e. the lack of a parasitophorous vacuole) has been shown to be wrong in many publications. furthermore, absolutely no ... | 2002 | 11936509 |
| seroprevalence of antibodies against neospora caninum in diagnostic equine serum samples and their possible association with fetal loss. | a case-control study of the association between the presence of serum antibodies against neospora spp. and fetal loss was performed on serum samples submitted to a veterinary diagnostic laboratory in northwestern united states. control sera were randomly selected from those submitted from healthy horses for routine equine infectious anemia testing required for regulatory health certification. case sera were randomly selected from those submitted from aborting mares for diagnostic workup. based o ... | 2002 | 11934465 |
| first confirmed case of neospora caninum-associated abortion outbreak in portugal. | in a dairy herd experiencing an abortion outbreak, 49% of the 119 cows were positive for neospora caninum antibodies and the remaining 51% were seronegative. using immunohistochemical analysis of foetal brain tissue, neospora cysts and bradyzoite clusters were identified in two of the 15 aborted foetuses submitted for testing. other agents that commonly cause abortions were not found. these results, coupled with neurological clinical symptoms in new-born calves, implicated n. caninum as the majo ... | 2001 | 11928926 |
| quantitative detection of neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time pcr. | we report the development of a real-time pcr assay for the quantitative detection of neospora caninum in infected host tissues. the assay uses the double-stranded dna-binding dye sybr green i to continuously monitor product formation. oligonucleotide primers were designed to amplify a 76-bp dna fragment corresponding to the nc5 sequence of n. caninum. a similar method was developed to quantify the 28s rrna host gene in order to compare the parasite load of different samples and to correct for th ... | 2002 | 11923330 |
| necrotizing cerebellitis due to neospora caninum infection in an old dog. | a severe, necrotizing, non-suppurative inflammation of the cerebellum associated with neospora caninum infection was identified in a 14-year-old male labrador retriever. on presentation, clinical signs included mild depression and head tremor, marked ataxia of both thoracic and pelvic limbs, and abnormal postural reactions. in the central nervous system, inflammatory lesions were mainly restricted to the cerebellar leptomeninges and cerebellar cortex, which appeared necrotic and atrophic. protoz ... | 2002 | 11913826 |
| effect of neospora caninum on in vitro development of preimplantation stage bovine embryos and adherence to the zona pellucida. | 2002 | 11913589 | |
| studies on serological cross-reaction of neospora caninum with toxoplasma gondii and hammondia heydorni. | the enzyme-linked immunosorbent assay (elisa) was used to examine cross-reactivity of neospora caninum with toxoplasma gondii and hammondia heydorni. anti-t. gondii mouse and cat sera cross-reacted with n. caninum soluble antigen (nla), but not with the recombinant surface antigen (ncsrs2). anti-h. heydorni dog sera showed no cross-reactivity with either the nla antigen or the ncsrs2. lack of cross-reactivity between anti-h. heydorni sera and n. caninum antigens, and the cross-reactivity of anti ... | 2002 | 11913555 |
| a method of probability diagnostic assignment that applies bayes theorem for use in serologic diagnostics, using an example of neospora caninum infection in cattle. | to develop a method of probability diagnostic assignment (pda) that uses continuous serologic measures and infection prevalence to estimate the probability of an animal being infected, using neospora caninum as an example. | 2002 | 11911564 |
| natural transmission routes of neospora caninum between farm dogs and cattle. | twelve dairy herds with evidence of post-natal infection with neospora caninum were compared with 21 control herds with no evidence of post-natal infection. on the former farms, dogs consumed placenta or licked uterine discharge in 75 and 67% of the farms, respectively, while on control farms these activities occurred in 38 and 24% of the farms, respectively. on all control farms and all but three post-natally infected farms the dogs were fed colostrum or milk. defecation of dogs on the feeding ... | 2002 | 11900923 |
| point source exposure of cattle to neospora caninum consistent with periods of common housing and feeding and related to the introduction of a dog. | eight dairy herds with evidence of post-natal transmission of neospora caninum were used to test the hypothesis of a point source exposure by a retrospective analysis of the housing and feeding of infected age-groups. the first n. caninum-associated abortion or birth of n. caninum-seropositive offspring from the post-natally infected age-group was considered as the first indication of the infection. in seven of the eight dairy herds, a point source exposure to n. caninum of the infected age-grou ... | 2002 | 11900922 |
| neosporosis with cerebellar involvement in an adult dog. | a case of an adult dog with multifocal, progressive neurological signs caused by neospora caninum is reported. pathological studies showed cerebellar lesions due to the parasite, which was also present in other parts of the nervous system and muscle. cerebellar atrophy related to neospora infection has been rarely reported in veterinary medicine, and has been shown to affect ruminants and dogs. the cerebellar involvement and the age of the present dog make this case uncommon. | 2002 | 11873953 |
| vaccine development against neospora caninum infection. | neospora caninum is a recognized protozoan parasite of a wide range of mammalian hosts, and was reported for the first time in 1988. the isolation of its oocysts in dog's faeces in 1998 led to its establishment as a parasitic species undergoing typical coccidian life cycle. infection with n. caninum causes paralysis and death in young livestock and companion animals, and is associated with abortions and stillbirth in cattle, and neurologic disease in calves. considering the economic and agricult ... | 2002 | 11853138 |
| neospora caninum and waddlia chondrophila strain 2032/99 in a septic stillborn calf. | characteristics of an intracellularly growing micro-organism isolated from an aborted bovine foetus are described. the organism replicated within cytoplasmic vacuoles, was resistant to penicillin and exhibited structural characteristics compatible with waddlia chondrophila. an elisa specific for chlamydia spp., immunofluorescence tests using antibodies directed against chlamydia spp. or simkania negevensis, and pcr using chlamydia-specific primers showed that the agent was distinct from chlamydi ... | 2002 | 11852195 |
| neospora 2001. | infection with the protozoan parasite neospora caninum is emerging as a major cause of reproductive loss in cattle worldwide. recent advances in research with the ultimate goal of devising effective and sustainable control measures were discussed at neospora 2001. neospora 2001 was held, 13-15 september 2001, at the moredun research institute, edinburgh, uk. the international meeting covered many aspects of bovine neosporosis including diagnosis, epidemiology and immunology. | 2002 | 11850000 |
| neospora caninum and hammondia heydorni are separate species/organisms. | neospora caninum and hammondia heydorni are two coccidian parasites with morphologically similar oocysts in canine feces. it was recently proposed that they are one species. in this paper, we review the biology and morphology of these parasites and present evidence that n. caninum and h. heydorni are separate species. | 2002 | 11832296 |
| in contrast to dogs, red foxes (vulpes vulpes) did not shed neospora caninum upon feeding of intermediate host tissues. | to clarify whether red foxes (vulpes vulpes) can be final hosts of neospora caninum, foxes and dogs were fed in parallel on tissues of a sheep and a goat experimentally infected with n. caninum. the faeces of at least two of five dogs contained n. caninum oocysts, as determined by bioassay. in the faeces of all six foxes fed in parallel, oocysts were detected that were larger in size (length 12.6 +/- 0.5 microm, width 11.8 +/- 0.4 microm) than the oocysts shed by the dogs. ribosomal rna sequence ... | 2002 | 11822736 |
| red foxes (vulpes vulpes) in ireland as hosts for parasites of potential zoonotic and veterinary significance. | intestinal washes, faecal flotations and serological examinations for antibodies to toxoplasma gondii and neospora caninum were used to assess the prevalence of parasites in carcases of foxes killed on roads or shot in the dublin area and surrounding counties. the ascarids uncinaria stenocephala and toxocara canis were prevalent, as was the trematode alaria alata. taenia species, eggs of capillaria species and sporocysts of sarcocystis species were also found. only one fox out of 70 examined was ... | 2001 | 11808662 |
| serological diagnosis of neosporosis in a herd of dairy cattle in southern brazil. | neospora caninum-specific antibodies were detected in 60 of 172 (34.8%) dairy cattle by enzyme-linked immunosorbent assay (elisa) in a herd from parana state, brazil. the seropositive animals included 47 of 126 (37.3%) adult cows, 7 of 29 (24%) heifers (1-2 yr), 4 of 15 (27%) heifers (5 mo-1 yr), and 2 precolostral samples. data collected over a 9-yr follow-up period revealed that the proportion of pregnancies ending in abortion was 20% (31/154) among elisa seropositive cows and 8% (15/193) amon ... | 2001 | 11780849 |
| isolation of neospora caninum from the brain of a pregnant sheep. | neospora caninum was isolated from the brain of a naturally infected pregnant sheep by inoculation of immunodeficient mice with a homogenate of the brain tissue. the ewe showed no clinical signs. tachyzoites were observed in the tissues of the nu/nu mice injected with the brain tissue homogenate and the diagnosis was confirmed by immunohistochemical staining with anti-n. caninum antibodies and by detecting n. caninum-specific dna by polymerase chain reaction. | 2001 | 11780846 |
| application of real-time fluorescent pcr for quantitative assessment of neospora caninum infections in organotypic slice cultures of rat central nervous system tissue. | the previously described nc5-specific pcr test for the diagnosis of neospora caninum infections was used to develop a quantitative pcr assay which allows the determination of infection intensities within different experimental and diagnostic sample groups. the quantitative pcr was performed by using a dual fluorescent hybridization probe system and the lightcycler instrument for online detection of amplified dna. this assay was successfully applied for demonstrating the parasite proliferation ki ... | 2002 | 11773124 |
| placentophagia--an alternative way for horizontal transmission of neospora caninum in cattle? | 2001 | 11756037 | |
| in vitro antiprotozoal effects of artemisinin on neospora caninum. | neospora caninum is an intracellular apicomplexan parasite that infects a wide range of mammals and has been associated with abortion in cattle worldwide. artemisinin is an effective antimalarial compound derived from a traditional chinese herbal remedy, qinghao or artemisia annua l. in the study reported, the cultured host cells (vero cells or mouse peritoneal macrophages) infected with n. caninum tachyzoites were incubated with alpha-mem (minimal essential medium) 10%hs supplemented with vario ... | 2002 | 11751000 |
| neosporosis as a cause of abortion in dairy cattle in rio grande do sul, southern brazil. | forty-six aborted bovine fetuses submitted to the faculty of veterinary, department of clinical pathology, universidade federal do rio grande do sul, were examined histopathologically. non-suppurative inflammation was observed mainly in the brain and heart of 22 fetuses. brain lesions consisted primarily of mononuclear inflammatory cell infiltrates with occasional foci of necrosis. protozoa that reacted with neospora caninum antisera were seen in 18 of the 22 (81.8%) brain specimens from fetuses ... | 2002 | 11750112 |
| bovine neosporosis: immunoblot improves foetal serology. | the purpose of the study is the comparative evaluation of the immunofluorescent antibody test (ifat) and an immunoblot (ib) test for the examination of foetal fluids for specific antibodies against neospora caninum. peritoneal and pleural fluids as well as abomasal contents were analysed. the results of the serological examinations were compared to those obtained by histological, immunohistochemical, and pcr analysis of foetal tissues as well as to the results of maternal serological examination ... | 2001 | 11731071 |
| neosporosis in bovine dairy herds from the west of france: detection of neospora caninum dna in aborted fetuses, seroepidemiology of n. caninum in cattle and dogs. | neospora caninum is considered one of the major causes of abortion in cattle in most parts of the world. in this study, the role of n. caninum was investigated in groups of aborted cattle and dairy herds from the west of france. good correlation was found between parasite dna detection in fetuses and serologic statuses of dams. in groups with documented abortion status and no antibodies to other pathogens, 17-45% of aborted animals were seropositive for n. caninum, and significant relationship b ... | 2001 | 11731070 |
| seroprevalences of toxoplasma gondii and neospora caninum in swedish red foxes (vulpes vulpes). | the prevalences of antibodies to the protozoan parasites toxoplasma gondii and neospora caninum were investigated by the direct agglutination test (dat) and elisa, respectively, in 221 red foxes (vulpes vulpes) from different parts of sweden. a total of 84 (38%) of the analysed sera had antibodies to t. gondii, but none of the foxes had antibodies to n. caninum. the results indicate that t. gondii infection is fairly common in swedish red foxes and that the infection is present in most parts of ... | 2001 | 11705663 |
| leishmania infantum and neospora caninum simultaneous skin infection in a young dog in italy. | leishmania infantum, the agent of canine leishmaniasis in mediterranean countries, and neospora caninum, a recently recognized protozoal pathogen in dogs, were diagnosed in a 9-month-old argentine dogo dog. both skin lesions and neurological signs were present. histopathology of cutaneous lesions revealed a suppurative, diffuse dermatitis with numerous intracellular protozoa. serology was positive for both l. infantum (1:640) and n. caninum (1:800). double-label immunohistochemical staining of s ... | 2001 | 11705654 |
| serodiagnosis of toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant sag1. | the gene encoding surface antigen 1 (sag1, p30) of toxoplasma gondii (t. gondii) was cloned into the plasmid pgex-4t-3 and subsequently expressed in escherichia coli (e. coli) as a glutathione-s-transferase (gst) fusion protein. the recombinant sag1 (rsag1) was refolded using 8m urea solution followed by dialysis and thereafter evaluated in an enzyme-linked immunosorbent assay (elisa) for serological diagnosis of toxoplasmosis. the test sera were adsorbed with gst to block non-specific reactivit ... | 2001 | 11705650 |
| a swiss case-control study to assess neospora caninum-associated bovine abortions by pcr, histopathology and serology. | neospora caninum is one of the most frequent infectious organisms causing abortion in cattle worldwide. the present case-control study was designed to assess the importance of bovine neosporosis for causing abortion in swiss cattle and to identify selected risk factors. infection was primarily diagnosed by a n. caninum-specific pcr and serology, complemented with histopathology and immunohistochemistry. a total of 113 case and 113 corresponding control-farms were studied for 1.5 year. during thi ... | 2001 | 11705647 |
| association of serologic status for neospora caninum and postweaning feed efficiency in beef steers. | to determine the effects of serologic status for neospora caninum on short-term weight gain, feed intake, and feed efficiency (feed intake/gain). | 2001 | 11697371 |
| prevalence of agglutinating antibodies to neospora caninum in raccoons, procyon lotor. | neospora caninum is an apicomplexan parasite that causes neonatal neuromuscular disease in dogs and abortions in cattle. dogs are the only proven definitive host. little is known about the prevalence of antibodies to this parasite in wildlife. sera from 99 raccoons (procyon lotor) were examined for agglutinating antibodies to n. caninum using the modified agglutination test employing formalin-fixed tachyzoites as antigen. raccoons originated in florida (n = 24, collected in 1996), new jersey (n ... | 2001 | 11695399 |
| comparative molecular investigation of nc5-pcr amplicons from neospora caninum nc-1 and hammondia heydorni-berlin-1996. | the clinical relevance of neospora caninum as a cyst-forming coccidian parasite is increasingly acknowledged within veterinary medicine, although the pathways of transmission are far from being solved. the parasite is well known for causing diaplacental infections in cows associated with abortion and/or severe damage of the fetus. in addition, it may cause neuromuscular disease in dogs, which thus apparently act as intermediate hosts as well as final hosts. in our previous studies, we have demon ... | 2001 | 11688897 |
| cyclic transmission of neospora caninum: serological findings in dogs shedding oocysts. | in a previous paper we demonstrated that hammondia heydorni-like oocysts isolated in 1996 from a naturally infected dog could not be distinguished from the isolate neospora caninum nc-1. the isolate, designated as h. heydorni-berlin-1996, was cyclically transmitted using dogs as the final hosts. the present study provides information on the antibody responses of the dogs used for the cyclical transmission of this isolate. the majority of dogs that had shed oocysts showed no sero-conversion with ... | 2001 | 11688895 |
| immune responses in pregnant cattle and bovine fetuses following experimental infection with neospora caninum. | humoral and cell-mediated immune (cmi) responses [i.e. proliferative responses and gamma interferon (ifn-gamma) production], were elicited in five cows infected between 159 and 169 days of gestation by a combined intravenous-intramuscular inoculation of neospora caninum tachyzoites. analysis of antigen-specific immunoglobulin (igg) subclasses revealed a predominant igg2 response in two cows, a mixed igg1-igg2 response in two other cows and a predominant igg1 response in one cow. no correlation w ... | 2001 | 11688887 |
| hammondia heydorni-like oocysts shed by a naturally infected dog and neospora caninum nc-1 cannot be distinguished. | this study describes transmission experiments using hammondia heydorni-like oocysts isolated in 1996 from a naturally infected dog. the isolate was designated as h. heydorni-berlin-1996. examination of sera from infected intermediate hosts showed immunoblot reactions that resembled patterns observed after neospora caninum nc-1 infection. furthermore, n. caninum dna could be demonstrated in tissue samples (e.g. heart, brain) of experimentally infected intermediate hosts and in oocyst preparations ... | 2001 | 11688886 |
| neospora caninum in persistently infected, pregnant cows: spontaneous transplacental infection is associated with an acute increase in maternal antibody. | nine cows which were naturally and persistently infected with neospora caninum were housed and observed intensively throughout pregnancy. no recrudescence of a latent infection was detected by pcr tests on maternal blood but fetal infection, implying a recrudescence of maternal parasitosis, was associated with a marked increase in maternal antibody. the increase occurred in the second half of pregnancy in five cows which infected their calves, and before mid-pregnancy in one cow which aborted. t ... | 2001 | 11688746 |
| small-subunit rdna sequencing of the italian bovine neospora caninum isolate (nc-pv1 strain). | the small-subunit (ssu) rdna of the neospora sp. nc-pv1 strain isolated in italy from cattle has been sequenced and compared to the other five n. caninum strains ssu rdna sequences deposited in the data bases. the nc-pv1 strain sequence is identical to three published sequences. minor differences, respectively four nucleotide bases and one nucleotide base, have been found when comparing the nc-pv1 sequence with two other available sequences of n. caninum. according to these results, the neospora ... | 2000 | 11686077 |
| characterization of neospora caninum surface protein ncsrs2 based on baculovirus expression system and its application for serodiagnosis of neospora infection. | the baculovirus expression system has proved to be a useful tool for the production of recombinant proteins. here we have characterized the neospora caninum surface protein ncsrs2 produced by two types of the recombinant virus and also have developed an enzyme-linked immunosorbent assay (elisa) using recombinant ncsrs2 for the serologic diagnosis of neospora infection. western blot analysis showed two major protein bands that were detectable in insect cells infected with each recombinant baculov ... | 2001 | 11682519 |
| validation of a commercially available monoclonal antibody-based competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to neospora caninum in cattle. | a previously described monoclonal antibody (mab)-based competitive-inhibition enzyme-linked immunosorbent assay (celisa) was modified to optimize performance, and the assay was validated in various defined cattle populations for detection of serum antibody to neospora caninum, a major cause of bovine abortion. modifications to the celisa included capturing native n. caninum antigen with a parasite-specific mab (mab 5b6-25) and directly conjugating the competitor mab (mab 4a4-2), with both mabs b ... | 2001 | 11682497 |
| protection against vertical transmission in bovine neosporosis. | in this study we were interested to determine whether infection of cattle prior to pregnancy would afford any protection to the foetus if the dams were challenged with neospora caninum at mid-gestation. the experiment comprised four groups of cattle: group 1, uninfected controls; group 2, inoculated with n. caninum tachyzoites 6 weeks prior to mating and then challenged with n. caninum at mid-gestation; group 3, naive cattle challenged with n. caninum at mid-gestation and group 4 were infected w ... | 2001 | 11595240 |
| determination of antigenic domain in gst fused major surface protein (nc-p43) of neospora caninum. | the antigenic domain of the major surface protein (nc-p43) of neospora caninum was examined by polymerase chain reaction of its gene fragments and recombinant expression as gst fusion proteins. the fragments of nc-p43 were as follow: a total open reading frame (ofr), t; ofr without signal sequence and c-terminal hydrophobic sequence, s; n-terminal 2/3 parts of s, a; c-terminal 2/3 parts, p; n-terminal 1/3 part, x; middle 1/3 part, y; and c-terminal 1/3 part, z, respectively. the dna fragments we ... | 2001 | 11590914 |
| isolation of neospora caninum from the brain of a naturally infected dog, and production of encysted bradyzoites in gerbils. | neospora caninum was isolated from the brain of an adult dog in brazil. cerebral tissue from the dog was inoculated into mongolian gerbils. gerbils were euthanized 3-4 months later and bradyzoite-containing tissue cysts were observed in their brains. n. caninum (designated nc-bahia) was isolated in cell culture after inoculation with tissue cysts from the gerbils. the identity of the parasite was confirmed by immunohistochemical examination and polymerase chain reaction (pcr). gerbils may be a u ... | 2001 | 11587828 |
| granulomatous encephalitis in a neurologically impaired goat kid associated with degeneration of neospora caninum tissue cysts. | congenital neospora caninum infection was diagnosed in a saanen goat from a farm in southern brazil. the kid was unable to nurse and had difficulty rising, ataxia, and opistothotonos. the neurologic signs became more severe 3 days after birth, when it was euthanized. no gross lesions were observed at necropsy. multifocal infiltrates primarily of mononuclear cells, nodular microgliosis, and perivascular cuffs of lymphocytes, plasma cells, and few neutrophils were observed in the brain, mostly in ... | 2001 | 11580064 |
| seroprevalence study of bovine neosporosis in mexico. | an indirect enzyme-linked immunosorbent assay was used to obtain epidemiologic information on bovine neosporosis in dairy herds of the mexican central plateau. sera were collected from 1,003 cows from 50 dairy herds. forty-three herds (group a) had been experiencing a high abortion rate. the abortion rates for the remaining 7 herds (group b) were within normal limits for mexico. five-hundred sixty-one (56%) of the 1,003 sera were positive. the seroprevalence of neospora caninum antibodies was 72 ... | 2001 | 11580063 |
| epidemiological pattern and risk factors associated with bovine viral-diarrhoea virus (bvdv) infection in a non-vaccinated dairy-cattle population from the asturias region of spain. | a survey of bovine viral-diarrhoea virus (bvdv) infection was carried out in a non-vaccinated cattle population from the asturias region of spain in 1997 to assess seroprevalence and identify risk factors associated with infection. twenty-eight herds were included; 529 cows were bled. information regarding the herd and each animal sampled were recorded through a personal interview with the farmer. the true prevalence was estimated to be 21%. according to the antibody-age profiles and the herd-ma ... | 2001 | 11566379 |
| ranch-management factors associated with antibody seropositivity for neospora caninum in consignments of beef calves in texas, usa. | a study was conducted with a 1998 retained-ownership population of texas (usa) beef calves to determine the ranch-management practices associated with calf seroprevalence to neospora caninum. management practices of 76 texas ranches that consigned 760 calves to a retained-ownership feedlot program were reviewed from a mailed questionnaire. ninety-nine of 760 (13%; 95% ci, 9.4%, 17.7%) calves were positive to n. caninum and 59% of the ranches consigned at least one positive calf. in the logistic ... | 2001 | 11566378 |
| protease activity and host cell binding of the 42-kda rhoptry protein from toxoplasma gondii after secretion. | three proteases were identified in the excretory/secretory proteins (esp) from toxoplasma gondii by the gelatin acrylamide gel electrophoresis (gage), of which the molecular masses were 80, 70, and 42 kda. one of the proteases with 42 kda was reactive to a monoclonal antibody (mab), tg786 clone, which was localized in the rhoptry of t. gondii by immunohistochemistry. the protease was maximally active at the ph range between 7.5 and 8.5, and was sensitive to inhibition by tpck and egta. the gelat ... | 2001 | 11563841 |
| neospora caninum microneme protein ncmic3: secretion, subcellular localization, and functional involvement in host cell interaction. | in apicomplexan parasites, host cell adhesion and subsequent invasion involve the sequential release of molecules originating from secretory organelles named micronemes, rhoptries, and dense granules. microneme proteins have been shown to be released at the onset of the initial contact between the parasite and the host cell and thus mediate and establish the physical interaction between the parasite and the host cell surface. this interaction most likely involves adhesive domains found within th ... | 2001 | 11553593 |
| association between exposure to neospora caninum and milk production in dairy cows. | to determine association between exposure to neospora caninum and milk production in dairy cows. | 2001 | 11549092 |
| oocyst excretion in dogs fed mouse brains containing tissue cysts of a cloned line of neospora caninum. | neospora caninum is an apicomplexan parasite that causes neonatal neuromuscular disease in dogs and abortions in cattle. bovine neosporosis is a major production problem worldwide. the parasite is transmitted to cattle via oocysts excreted by dogs or by transplacental transmission. dogs are the only proven definitive host for n. caninum. one of 3 dogs fed mouse brains containing tissue cysts of a wild-type n. caninum strain ck0160sc3b (cko) excreted oocysts in its feces. two of 3 dogs fed mouse ... | 2001 | 11534658 |
| prevalence of neospora caninum infection in dogs from beef-cattle farms, dairy farms, and from urban areas of argentina. | prevalence of anti-neospora caninum antibodies was determined in sera of 320 dogs from argentina using the indirect fluorescent antibody test (ifat). antibodies to n. caninum were found in 121 of 320 (37.8%) sera with titers of 1:50 (21 dogs), 1:100 (23 dogs), 1:200 (23 dogs), 1:400 (17 dogs), 1:800 (23 dogs), and > or = 1:1.600 (14 dogs). the seropositivity (ifat, > or = 1:50) was higher in dogs from dairy (48% of 125) and beef (54.2% of 35) farms than in dogs from urban areas (26.2% of 160). p ... | 2001 | 11534656 |
| isolation and characterization of two parasitic protozoa from a pacific harbor seal (phoca vitulina richardsi) with meningoencephalomyelitis. | two species of protozoans were isolated from a harbor seal with fatal meninogoencephalitis. serologic reactivity was detected to both sarcocystis neurona and toxoplasma gondii. parasites associated with brain inflammation and necrosis reacted only with immunohistochemical stains utilizing polyclonal antisera raised against sarcocystis neurona. however, 2 distinct parasites were observed in cell cultures derived from the seal's brain tissue. these parasites were separated by mouse passage and lim ... | 2001 | 11534647 |
| tgm2ap participates in toxoplasma gondii invasion of host cells and is tightly associated with the adhesive protein tgmic2. | like other members of the medically important phylum apicomplexa, toxoplasma gondii is an obligate intracellular parasite that secretes several classes of proteins involved in the active invasion of target host cells. proteins in apical secretory organelles known as micronemes have been strongly implicated in parasite attachment to host cells. tgmic2 is a microneme protein with multiple adhesive domains that bind target cells and is mobilized onto the parasite surface during parasite attachment. ... | 2001 | 11532123 |
| seroprevalence of neospora caninum in female water buffaloes (bubalus bubalis) from the southeastern region of brazil. | antibodies to neospora caninum were assayed in sera of 222 female water buffaloes from ribeira valley of são paulo state, brazil, using an indirect fluorescent antibody test (ifat) and neospora agglutination test (nat). ifat antibodies were found in 64% of buffaloes with titers of 1:25 (42 buffaloes), 1:50 (53 buffaloes), 1:100 (31 buffaloes), 1:200 (10 buffaloes), 1:400 (3 buffaloes), or > or =1:800 (3 buffaloes). nat antibodies were found in 53% of buffaloes; in titers of 1:40 in 52 buffaloes, ... | 2001 | 11511420 |
| cutaneous neosporosis in two adult dogs on chronic immunosuppressive therapy. | antemortem diagnosis of generalized ulcerative and pyogranulomatous dermatitis with numerous intralesional tachyzoites was made from skin biopsy specimens from 2 adult dogs on chronic immunosuppressive therapy. a 9-year-old italian greyhound was on long-term corticosteroid therapy for the treatment of a lupus-like systemic autoimmune disorder, and a 7-year-old labrador retriever had received several months of chemotherapy for lymphosarcoma. the tachyzoites were identified as neospora caninum by ... | 2001 | 11482605 |
| interferon-gamma-induced apoptosis in host cells infected with neospora caninum. | interferon-gamma (ifn-gamma) has a crucial role for host defence against parasite infection. it is not clear, however, how ifn-gamma affects the parasite-infected host cells. the effect of ifn-gamma on neospora caninum-infected cells was investigated in murine fibroblasts and canine kidney cells in vitro. in the presence of ifn-gamma, the viability of the infected host cell was decreased and apoptotic cell death occurred, as analysed by dna stainings with propidium iodide and a terminal deoxy-nu ... | 2001 | 11467780 |
| genetic and biological diversity among isolates of neospora caninum. | neospora caninum is a protozoan parasite that causes bovine abortion. the epidemiology of n. caninum is poorly understood and little is known about the genetic diversity of the parasite, or whether individual isolates differ in virulence. such diversity may, among other factors, underlie the range of pathologies seen in cattle. in this study we analysed biological and genetic variation in 6 isolates of n. caninum originating from canine and bovine hosts by measurement of growth rate in vitro, we ... | 2001 | 11467779 |
| prevalence of neospora caninum antibodies in wild ruminants from the italian alps. | 2001 | 11442237 | |
| neosporosis in mexican dairy herds: lesions and immunohistochemical detection of neospora caninum in fetuses. | of 211 aborted bovine fetuses collected from mexican dairy herds between january 1996 and march 1999, 73 showed microscopical lesions consistent with neosporosis. of these 73 fetuses, 58 (79%) showed lymphocytic myocarditis, 39 (53%) showed microgliosis and multifocal necrosis in the brain, 39 (53%) showed lymphocytic hepatitis, and 19 (26%) showed lymphocytic myositis. immunohistochemical examination of brain, myocardium and liver from 53 of the same 73 fetuses demonstrated neospora caninum ant ... | 2001 | 11437517 |
| immunohistochemical and ultrastructural evidence for neospora caninum tissue cysts in skeletal muscles of naturally infected dogs and cattle. | protozoan stages were detected in the skeletal muscles of four dogs suffering from neosporosis and two neonatal calves with confirmed neospora caninum- infection which could be immunohistochemically labelled by an antiserum against the bradyzoite-specific antigen bag-5. in one calf, a tissue cyst was labelled by an antiserum against the n. caninum isolate nc-1. ultrastructurally, a 0.3-1 microm-thick cyst wall surrounded the labelled parasites. the cysts were located within myofibres and contain ... | 2001 | 11429181 |
| in the absence of endogenous gamma interferon, mice acutely infected with neospora caninum succumb to a lethal immune response characterized by inactivation of peritoneal macrophages. | following infection with neospora caninum, balb/c mice were shown to be resistant to an acute infection but developed a latent chronic infection. however, balb/c background gamma interferon (ifn-gamma)-deficient mice were sensitive to the acute infection. since the immune response in ifn-gamma-deficient mice is scantly known, we examined the function of macrophages, major histocompatibility complex (mhc) class ii expression, t-cell responses, and serum cytokine levels in the mice. all ifn-gamma- ... | 2001 | 11427432 |
| first isolation of neospora caninum from the feces of a naturally infected dog. | neospora caninum is a major cause of abortion in cattle worldwide. cattle become infected with n. caninum by ingesting oocysts from the environment or transplacentally from dam to fetus. experimentally, dogs can act as definitive hosts, but dogs excrete few oocysts after ingesting tissue cysts. a natural definitive host was unknown until now. in the present study, n. caninum was isolated from the feces of a dog. gerbils (meriones unguiculatus) fed feces from the dog developed antibodies to n. ca ... | 2001 | 11426726 |
| detection of igg antibodies to neospora caninum and toxoplasma gondii in dogs examined in a veterinary hospital from brazil. | a total of 163 dogs with neuromuscular, respiratory and/or gastrointestinal disorders, was admitted at the veterinary hospital, federal university of uberlândia, brazil, and submitted to serology for toxoplasma gondii and neospora caninum. assays for t. gondii included indirect haemagglutination (iha), indirect fluorescent antibody (ifat-tg), immunoenzymatic (elisa), and immunoblotting (ib-tg). assays for n. caninum included ifat-nc and immunoprecipitation (ip-nc). based on concordant results by ... | 2001 | 11423182 |
| dogs shed neospora caninum oocysts after ingestion of naturally infected bovine placenta but not after ingestion of colostrum spiked with neospora caninum tachyzoites. | an experiment was carried out to determine whether bovine colostrum or placenta could be a source of infection of neospora caninum for dogs. for this purpose, two dogs were fed bovine colostrum to which culture-derived n. caninum tachyzoites were added and two other dogs were fed placental cotyledonary tissue from n. caninum seropositive cows. one dog served as a negative control during the start of the experiment but this control dog was fed cotyledonary tissue later on. none of the dogs did pr ... | 2001 | 11403764 |
| bovine abortion associated with neospora caninum in hungary. | the authors report the first case of bovine neosporosis in hungary based on investigations made on an aborted fetus. the diagnostic methods included traditional as well as molecular techniques. this record extends further the geographic range of the disease. | 2001 | 11402646 |
| evaluation of the embryo transfer procedure proposed by the international embryo transfer society as a method of controlling vertical transmission of neospora caninum in cattle. | to evaluate efficacy of embryo transfer into seronegative recipients, using the procedure proposed by the international embryo transfer society (iets), for preventing vertical transmission of neospora caninum in cattle. | 2001 | 11394835 |
| reproductive performance of a cow-calf herd following a neospora caninum-associated abortion epidemic. | this study examines the long-term impact of a neospora caninum-associated abortion outbreak in a large cow-calf herd in northern alberta. blood samples were collected 4 times from all bred females and heifer calves born during the spring before the outbreak: (1) at the time of the outbreak, (2) the following spring, (3) the subsequent fall, and, finally, (4) the second spring after the outbreak. the samples were analyzed using a commercially available enzyme-linked immunosorbent assay for n. can ... | 2001 | 11360856 |
| prevalence of antibodies to neospora caninum and toxoplasma gondii in gray foxes (urocyon cinereoargenteus) from south carolina. | little is known about the epidemiology of neospora caninum in wild mammal populations. it has been suggested that a sylvatic cycle exists for n. caninum. dogs and potentially other canids are a definitive host for n. caninum. the present study was done to determine the prevalence of antibodies to n. caninum in a population of gray foxes (urocyon cinereoargenteus) from a nonagricultural setting in south carolina. we also determined the prevalence of antibodies to toxoplasma gondii in these animal ... | 2001 | 11358632 |
| neospora caninum infection associated with stillbirths in captive antelopes (tragelaphus imberbis). | neosporosis was diagnosed in full-term stillborn twin calves of captive antelopes (tragelaphus imberbis) in a german zoo. in both calves a multifocal non-suppurative encephalitis was present and infection with neospora (n.) caninum was confirmed by foetal serology and pcr. n. caninum-dna was detected in brain, heart, lung, liver and spleen of a third full-term stillborn calf. histopathological lesions were absent in this calf and antibodies against n. caninum were not detected in pleural fluid. ... | 2001 | 11358631 |
| failure of dogs to shed oocysts after being fed bovine fetuses naturally infected by neospora caninum. | neospora caninum is a protozoan that causes abortion in cattle. the dog has recently been identified as a definitive host for n. caninum. to verify if bovine fetuses can infect dogs, nine 2-4-month-old dogs were fed bovine fetuses naturally infected by n. caninum. none of the dogs excreted oocysts, seroconverted, had clinical signs or lesions compatible with n. caninum infection. additional studies will be necessary to determine the natural mode of infection of dogs by n. caninum. | 2001 | 11358630 |
| experimental studies on the transmission of neospora caninum between cattle. | three studies were conducted to investigate the transmission of neospora caninum between cattle by the oral route. in the first study, six calves were dosed with 10(7)n caninum tachyzoites (nc livb1) in colostrum and/or milk replacer on four occasions. in the second study, two calves and two cows were fed placental tissues from n caninum -infected cows, and, in the third study, seven uninfected calves were fostered onto n caninum -infected dams. in the first study, all six calves developed antib ... | 2001 | 11356096 |
| comparison of the growth inhibitory effects of canine ifn-alpha, -beta and -gamma on canine cells infected with neospora caninum tachyzoites. | the growth inhibitory effects of recombinant canine interferon alpha (ifn-alpha), beta (ifn-beta) and gamma (ifn-gamma) were examined on madin-darby canine kidney cells infected with neospora caninum tachyzoites. the parasite growth was inhibited by all ifns in a dose-dependent manner. ifn-gamma inhibited the parasite growth with greater efficacy than ifn-alpha or ifn-beta. moreover, the effect of ifns on n. caninum growth associated with the suppression of the host cell viability. the present s ... | 2001 | 11346181 |
| naturally-occurring neospora caninum infection in an adult sheep and her twin fetuses. | neospora caninum tissue cysts were found in the brains of surgically delivered twin fetuses at 119 days of gestation. in the brains of both fetuses, there was an inflammatory reaction involving perivascular cuffings of mononuclear cells, glial nodules. the dam of these fetuses died because of metritis. histopathological examination of the ewe revealed n. caninum tissue cysts and focal gliosis with mononuclear cell cuffings. a n. caninum-specific dna fragment was detected in a brain homogenate of ... | 2001 | 11318580 |