Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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a small rna virus with a divided genome from heteronychus arator (f.) [coleoperai scarabaeidae]. | some properties of a small rna virus isolated from larvae of heteronychus arator [coleoptera: scarabaeidae] are described. the virus develops in the cytoplasm of gut and fat body cells and is 30 nm in diamter. the sedimentation coefficient of virus was 137s and the buoyant density was 1-33 g/ml. the single stranded rna was isolated as two species which sedimented at 22s and 15s, corresponding to mol. wt. of 1-0 and 0-5 x 10(6) respectively. the base ratios of the two rna species were 22 s: a=29- ... | 1976 | 978185 |
the allatotropic activity of the larval brain of galleria mellonella cultured in vitro. | 1976 | 955387 | |
ultrastructure of the neurosecretory cells of the pars intercerebralis of galleria mellonella (lepidoptera) after noradrenaline administration. | 1977 | 924116 | |
the purification by affinity chromatography of a glutathione s-transferase from larvae of galleria mellonella. | 1977 | 834106 | |
[host range of drosophila melanogaster c virus among diptera and lepidoptera (author's transl)]. | the host range of the c picornavirus of drosophila melanogaster was studied, using numerous strains of drosophila together with four other genera of diptera and two species of lepidoptera. c virus was injected into the different hosts and serially passaged in them. the extracts from each passage were biologically assayed on virus free d. melanogaster. four different situations were found. 1) a high level of multiplication leading, in 45 strains of drosophilidae, to the early death of the hosts. ... | 1976 | 823856 |
[reestablishment of the molt by brain implantation in the permanent larvae of galleria mellonella]. | postcephalic ligatures of larvae of galleria mellonella resulted in the production of permanent larvae. implantation of brains into such permanent larvae restored moulting. the percentage of successful reactivation of permanent larvae depended on the number of implanted brains. | 1976 | 816532 |
effect of cobra venom factor on the in vivo immune response in galleria mellonella to pseudomonas aeruginosa. | 1976 | 815440 | |
the pathogenicity of rough strains of pseudomonas aeruginosa for galleria mellonella. | rough mutants of two strains of pseudomonas aeruginosa have been isolated by selection for resistance to virulent lipopolysaccharide-specific phages. the rough mutants fell into two classes on the basis of colonial morphology and agglutination by acriflavine and nacl. the pigment and exoenzyme-synthesizing properties of these derivatives were identical with those of the parental cultures. the rough strains were 8- to 62-fold less pathogenic for the larvae of the wax moth (galleria mellonella) th ... | 1975 | 814978 |
[indirect effect of the larval food of an alternative host (galleria mellonella l., lepidoptera, galleriidae) on the reproduction of an entomophagous insect (lixophaga distraeae towns, dipt. tachinidae)]. | g. mellonella reared on its natural food, beeswax and pollen, is not suitable for a continuous rearing of l. diatraeae. as a matter of fact, l. diatraeae cannot be maintained for more than 10 generations on g. mellonella because the effect of adverse factors during the larval stage progressively reduces both mating rate and female fertility. but adding vitamin e to the natural larval food of g. mellonella eliminates these reproduction disturbances of the parasite, and l. diatraeae rearing can be ... | 1975 | 813895 |
[occurrence and function of a proteinase inhibitor in the hemolymph of insects]. | the presence of proteinase inhibitor has been proved in the hemolymph of a number of insect species from seven different insect orders. the amount of proteinase inhibitor in the hemolymph significantly increases after injection of inactivated bacteria into the hemocoelom of galleria mellonella-larvae. moreover the larvae show an increased resistance against normally lethal concentrations of trypsin, chymotrypsin, pronase p and extracellular proteinase produced by pseudomonas aeruginosa. it is di ... | 1975 | 811027 |
[virus disease complexes: transmissible pathological entities in invertebrates]. | virus disease complexes of galleria mellonella l. due respectively to a parvovirus with a baculovirus and a parovirus with an iridovirus have been transmitted to healthy larvae by ingestion of corpses of larvae affected by these disease complexes. the histological and cytological injuries observed are identical to those noted during the study of the initial complexes. | 1975 | 807417 |
[fractionation and amino-acid composition of acid-soluble trypsin of inclusion bodies protein of nuclear polyhedrosis virus of moth, galleria mellonella]. | a mixture of ph 3.5-soluble trypsin peptides of inclusion bodies proteins of moth nuclear polyedrosis virus was fractionated by means of ionic exchange chromatography on dowex 50x2 and purified by paper electrophoresis-chromatography. 32 peptides are isolated, their amino acid composition being studied. | 1976 | 799518 |
[study of the homology of nuclear and polysomal virus-specific rna in nuclear polyhedrosis of galleria mellonella l]. | 2006 | 765687 | |
reprogramming and dna synthesis in galleria mellonella larval epidermal cells. | epidermal cells from ligated day-3 last instar larvae of galleria mellonella secrete a larval cuticle if exposed to ecdysone immediately following ligation; whereas, they deposit a pupal cuticle if exposed to ecdysone three to four days after ligation. during the period of reprogramming the genome in the diploid cells of the wax moth larvae no dna synthesis occurs. this is direct proof that dna synthesis is not required for reprogramming in the epidermis of galleria during larval-pupal transform ... | 1978 | 750267 |
ultrastructural analysis of the action of reserpine on the brain neuroendocrine system of the wax moth, galleria mellonella l., lepidoptera. | this study concerns the influence of reserpine on the fine structure of peptidergic neurosecretory cells in the pars intercerebralis of galleria mellonella, and of neurons containing smaller dense-cored vesicles (presumed to be aminergic) localized in the same area of the brain. the drug, administered in doses of 125 microgram and 250 microgram per g of insect body weight, reduces both the amount and the electron opacity of the dense-cored vesicles with a diameter of 60--80 nm in the neuronal pe ... | 1978 | 688371 |
steroid transport through the surface of the prothoracic gland cells in galleria mellonella l. | steroid transport through the cell surface of the giant polyploid prothoracic gland cells of galleria mellonella l. was studied by an ultracytochemical method. the alkaloid digitonin, known to form a complex with all sterols having a free-oh radical in position 3, proved to be suitable for studying the interiorisation of moulting hormone precursors and the release of synthesized hormones. the results suggest that cholesterol uptake in the last larval instar occurs by macropinocytosis during the ... | 1978 | 639089 |
changes in ecdysone content during postembryonic development of the wax moth, galleria mellonella: the role of the ovary. | 1978 | 631539 | |
morphogenesis of the nodamura virus in the larbae of the lepidopteran galleria mellonella (l.). | the pathogenesis and morphogenesis of the nodamura virus, an insect picorna- virus which can also infect vertebrates such as newborn mice, are described in the larvae of g. mellonella. examination of thin sections of muscle, salivary and moulting glands, hemocytes adipose tissue and hypodermis of the infected larvae of g. mellonella shows in the cytoplasm the accumulation of viral particles either dispersed or in a stringlike or paracrystalline array. these arrays of virions can be within membra ... | 1978 | 626593 |
monoamine-containing cells in the brain of galleria mellonella (lepidoptera). | 1977 | 610395 | |
ultrastructure of the protocerebral neurosecretory cells of larval galleria mellonella, in situ and after culture of the brain in vitro. | three major groups of neurosecretory cells are described in the larval brain of galleria mellonella at two different times during the last larval instar and in larval brains after 72 hr of culture in vitro. the medial group in vivo consists of four distinct neurosecretory cell types, based on characteristic size and morphology, while the posterior and lateral groups each contain a single distinct type of neurosecretory cell. morphological differences between the same neurosecretory cells at the ... | 1977 | 610004 |
defensive reactions of galleria mellonella l. catepillars to the effect of herbicides and the nematode neoaplectana carpocapsae weiser. | 1977 | 564232 | |
comparative levels of lysozyme activity in larvae and pupae of galleria mellonella after particulate and soluble materials injection. | using an assay system for lysozyme activity determination in which haemolymph was compared in activity to known standards, the quantitative shifts in the lysozyme activity level of larvae and pupae of galleria mellonella, after particulate and soluble materials injection, were compared. the level of this activity in all injected larvae was higher fro that of pupae, but the immune response of pupae was more effective in all treatments when compared with activity in the non-injected controls. furt ... | 1979 | 552308 |
ultrastructural changes in the ovaries of the wax moth, galleria mellonella l. (lepidoptera), after treatment with reserpine. | 1979 | 546096 | |
the neural lamella of the wax moth galleria mellonella l. (lepidoptera) brain. | 1979 | 546092 | |
phagocytosis-stimulating mediators in insects. | the ability of insect blood cells to ingest all kinds of synthetic particles and also a wide range of microorganisms in a very short time after injection has up to now been regarded as a phagocytic function without any humoral mediators. in a phagocytosis model with latex beads and nonhagocytosable cells of bacillus thuringiensis subtoxicus, we are able to demonstrate the existence of lymphokine-like factors, which intervene in cellular defence reactions of insect. the following results were obt ... | 1979 | 543373 |
[improved methods for the mass breeding of greater wax moth larvae (galleria mellonella l.)]. | 1979 | 516927 | |
effect of reserpine on the larval-pupal moult of the wax moth galleria mellonella l. (lepidoptera). | significant retardation of g. mellonella development was induced by reserpine injected to the last instar larvae in doses having a long-lasting effect on the neurosecretory system. | 1979 | 510494 |
an ultrastructural and cytochemical study of the interaction between latex particles and the haemocytes of the wax moth galleria mellonella in vitro. | the plasmatocytes are the major phagocytic blood-cell type in the haemolymph of the wax-moth, galleria mellonella. in the present study, these cells were allowed to attach to tissue culture dishes for 1 h, rinsed and then incubated with latex beads for up to 72 h. these cells were then fixed for routine transmission electron microscopy and acid phosphatase cytochemistry. intracellular latex particles were found in tight, ill-defined phagosomes, which were often clearly associated with the golgi ... | 1979 | 466691 |
[studies on the activation of humoral defense factors against bacteria in larvae of the wax moth galleria mellonella l]. | the change of lysozyme activity in the hemolymph of the wax moth larvae caused by vaccination is influenced by the size and the dose of the injected particles. the rate of incorporation of those particles into phagocytes has no effect on this process. the change of lysozyme activity correlats negatively with the amount of granulocytes after vaccination. there were no correlations with the amount of other types of the hemocytes, neither with the resistance of the larvae against pseudomonas aerugi ... | 1977 | 410207 |
studies on the in vivo cellular reactions of insects: an ultrastructural analysis of nodule formation in galleria mellonella. | cellular aggregates or nodules which formed in galleria mellonella larvae in response to injections of killed bacillus cereus were examined during their first 24 hr of development. the initial aggregation stage was very rapid, occurring within 1 min, and was brought about by the release of an adhesive flocculent material by granular haemocytes in contact with the bacteria. this material, which subsequently became increasingly electron dense owing to melanin deposition, consolidated and by 5 min ... | 1977 | 408940 |
non-occluded particles of nuclear polyhedrosis virus infecting galleria mellonella l.: titration in vivo and in vitro. brief report. | infectivity of non-occluded particles of nuclear polyhedrosis virus infecting galleria mellonella l. was determined with bioassay technique in vivo and with plaque assay method in vitro using scld 135 (quiot) established cell culture. a plaque-forming virus unit corresponds to about 50 id50 for galleria larvae injected into hemocoel for non-cloned and 0.1-1.0 id50 for a virus isolate cloned from an infective plaque. | 1979 | 391180 |
electron microscopical-immunocytochemical evidence of ecdysteroids in the prothoracic gland of galleria mellonella. | fixation of prothoracic glands of galleria mellonella with a solution containing saponin permits immunocytochemical staining of the entire gland. by this means ecdysteroids were demonstrated electron microscopically to be present in the hyaloplasm and microtubules. | 1979 | 385145 |
macromolecular structure of nuclear polyhedrosis virus genome. | dna preparations from nuclear polyhedrosis virus (npv) of galleria mellonella l. (gml) were fractionated in high ionic strength neutral sucrose gradient. this procedure allowed a separation of supercoiled infectious dna molecules with contour length of 48--52 microns from infectious open ring dna molecule, and noninfectious linear dna molecules of the same size. in addition a heterogeneity of supercoiled dna molecules was detected. covalently closed dna molecules did not contain protein or ribon ... | 1979 | 378185 |
the encapsulation of foreign tissue implants in galleria mellonella larvae. | 1977 | 323370 | |
supercoiled dna of nuclear polyhedrosis virus of galleria mellonella l. | supercoiled infectious dna was isolated from nuclear polyhedrosis virus infecting great wax moth galleria mellonella l.) covalently closed dna molecules constitute approximately 10--30 per cent in our preparations. these molecules dissappear during storage. electron micrographs of supercoiled and open circular molecules are presented. length of the open rings is about 50--52 micron. infectivity of different dna forms is discussed. | 1977 | 322646 |
the fragments from the 28 s ribosomal rna of galleria mellonella with unesterified uridine at the 3'-termini. | the three 3'-ends of the 28 s galleria rna are all unesterified uridines. the two 18-s products due to the primary nick of the 28 s rna have a similar 3'-terminal dinucleotide: g-uoh. all of the seven species of secondary products from the galleria 28 s rna were suggested to have unesterified uridines, in common, at the 3'-ends. these results raise the possibility that the enzyme concerned in generating the primary and secondary nicks is a uracil-specific and 5'-phosphate-forming phosphodiestera ... | 1976 | 175853 |
the ultrastructure and ultracytochemistry of the basement membrane of the galleria mellonella fat body. | the fat body lobes of galleria mellonella are surrounded by basement membrane - a fine granular layer of connective tissue. this membrane has an affinity for ruthernium red. the results obtained after treatment of the fat body with neuraminidase, hyaluronidase, phospholipase c and proteolytic enzymes suggest that glycoproteins and phospholipoproteins are constituents of this basement membrane. the basement membrane also has the ability to bind concanavalin a-peroxidase, which is associated with ... | 1977 | 137774 |
ruthenium red staining of the neural lamella of the brain of galleria mellonella. | the outer surface of the neural lamella, the connective tissue ensheathing the brain, shows the ability to bind ruthenium red in the wax moth larva. ruthenium red-positive material is sensitive to neuraminidase, hyaluronidase and to some extent to phospholipase c, what suggests that the negative charge on the external surface of the neural lamella depends on the presence of the anionic groups of sialic and hyaluronic acids and phospholipids. | 1977 | 137773 |
gut flora of galleria mellonella suppressing ingested bacteria. | 1979 | 119813 | |
time and dose studies of the effect of cobra venom factor on the in vivo immune response in galleria mellonella to pseudomonas aeruginosa. | the induced resistance of wax moth larvae vaccinated with formolized pseudomonas aeruginosa 18 hr prior to challenge with the live organism was significantly inhibited by 0.2 to 1.0 units of cobra venom factor (cvf) per insect given 6 hr after vaccination. approximately 90% inhibition of the protective response was caused by 1.0 units cvf/insect. administration of this dose 6 hr before vaccination, with the vaccine, and 6 hr before challenge had no significant effect. the cvf effect was inhibite ... | 1978 | 99334 |
polypeptide antibiotic 26a from bacillus subtilis. i. taxonomy and fermentative production. | in surface cultures on nk/2-sym's medium, the isolate no. 26a of bacillus subtilis from the intestinal tract of galleria mellonella larvae produced three antibacterial substances which were separated by gel filtration on sephadex g-25 column. the major bioactive compound named 26a had a close resemblance to bacitracin family of polypeptide antibiotics. two minor active compounds, i.e. a bacteriolytic enzyme with endo-beta-n-acetylmuramidglycanohydrolase (ec. 3. 2. 1. 17) activity and other unide ... | 1978 | 81595 |
an iridovirus from bees. | an iridovirus, apis iridescent virus (aiv), isolated from sick adult specimens of apis cerana (hymenoptera) from kashmir, closely resembles iridescent viruses from tipula and sericesthis spp. (tiv and siv). however, aiv is only distantly related serologically to tiv and siv and is even more remotely related to several other similar viruses that were tested in tube precipitation tests with intact particles. aiv multiplies in apis mellifera, forming cytoplasmic iridescent crystalline aggregates in ... | 1976 | 58966 |
[the cellular reticular structures induced by baculovirus in the lepidoptera galleria mellonella]. | induction of filamentous nuclear structures by baculoviruses are observed in the hemocytes of galleria mellonella larvae. modalities of detection of these virus-induced structures by means of sheep or rabbit globulines labelled with fluorescein isothiocyanate are described. | 1975 | 50151 |
[comparative biochemical studies of polyhedral proteins of nuclear polyhedrosis viruses]. | using disc polyacrylamide gel electrophoresis, the molecular weights of polyhedral proteins of nuclear polyhedrosis viruses (npv) of porthetria dispar, mamestra brassicae, and aporia crataegi were found to be 28000 +/- 3000. it was shown that npv polyhedra of bombyx mori, galleria mellonella, p. dispar, and m. brassicae contain a protease. during dissolution of the polyhedra at ph 10,5 this protease specifically cleaves the matrix protein into 2--5 fragments. the amino acid compositions of npv p ... | 1978 | 33725 |
proteins of bacillus thuringiensis delta-endotoxin crystals. | pure crystals (at least 99% purification) of sigma-endotoxin were isolated from bac. thuringiensis var. galleriae. the complete dissolution of crystals might be achieved by the increase of ph up to 12 and higher or by a combined action of s = s-reducing and denaturing agents. electrophoresis of the solubilized crystal proteins in 5% polyacrylamide gels containing 0,1% sodium dodecyl sulfate and 8 m urea reveals two major bands corresponding to molecular weights of 120000--140000 (65%) and 65000 ... | 1977 | 21000 |
purification of free polyhedrosis virus infecting galleria mellonella l. | free galleria mellonella nuclear polyhedrosis virus was purified 4000--4500 times by tween-80 treatment and repeated sucrose density gradient centrifugation, based on the ratio of infectivity to protein content as compared to the starting virus-containing suspension. | 1977 | 17285 |
exocellular proteases of serratia marcescens and their toxicity to larvae of galleria mellonella. | out of 18 strains of serratia marcescens producing exocellular proteases the strain serratia marcescens cceb 415 was selected according to preliminary experiments. it could be shown that the train exhibits proteolytic activity reaching up to 10 tu per 1 ml of the culture filtrate in a medium with gelatine and peptone. two proteolytic enzyme could be demonstrated by means of specific inhibitors edta and diisopropyfluorophosphate: metalprotease with optimum activity at ph 7.5 and serine protease w ... | 1976 | 11154 |
studies on insect bacteriolytic enzymes--ii. some physical and enzymatic properties of lysozyme from haemolymph of galleria mellonella. | 1976 | 9261 | |
the degradation of the cuticle proteins of greater wax moth larvae (galleria mellonella) by toxic proteolytic enzymes, secreted by the entomophagous fungus beauveria bassiana (author's transl). | 1976 | 8934 |