Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
bacterial population of a two-phase anaerobic digestion process treating effluent of cassava starch factory.different groups of microorganisms in a two-phase anaerobic system were enumerated to evaluate the prevalence of specific groups and species. total and fecal coliforms showed similar values both in acidogenic and methanogenic reactors. the fecal streptococci were 4-fold higher in the acidogenic reactor, when compared with those of the methanogenic reactor. as expected, no methane forming or sulfate reducing bacteria were found in the acidogenic reactor. the populations of methanogenic bacteria w ...200212088382
co-adaptation between cassava mosaic geminiviruses and their local vector populations.four cassava mosaic geminivirus (cmg) isolates; african cassava mosaic virus from namulonge, uganda (acmv-[nam]), east african cassava mosaic virus from mtwara, tanzania (eacmv-[mtw]), eacmv-uganda from namulonge (eacmv-ug[nam]) and indian cassava mosaic virus from trivandrum, india (icmv-[tri]) were compared for their ability to be transmitted by four colonies of cassava whitefly, bemisia tabaci (gennadius), collected from namulonge (nam), mtwara (mtw), kumasi (kum) and trivandrum (tri). with 2 ...200212076831
a distinct bemisia tabaci (gennadius) (hemiptera: sternorrhyncha: aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in uganda.during the 1990s, an epidemic of cassava mosaic virus disease caused major losses to cassava production in uganda. two factors associated with the epidemic were the occurrence of a novel recombinant begomovirus, eacmv-ug, and unusually high populations of the whitefly vector, bemisia tabaci. here we present molecular evidence for the occurrence of two cassava-colonizing b. tabaci genotype clusters, ug1 and ug2, one of which, ug2, can be consistently associated with the cmd epidemic in uganda at ...200212074729
cassava diet--a cause for mucopolysaccharidosis?studies were carried out to determine the changes in glycosaminnoglycan (gag) metabolism in rats fed cassava with varying cyanoglucoside levels and two levels of protein. results indicated that there was an enhancement in the level of total and individual gag with a corresponding reduction in the activity of enzymes involved in the degradation of glycosaminoglycan. these changes were significant for rats given a cassava diet (raw and boiled cassava) and low protein. the changes in total and indi ...200212049146
food safety and amino acid balance in processed cassava "cossettes".processed cassava (manihot esculenta crantz) roots provide more than 60% of the daily energy intake for the population of the democratic republic of congo. insufficiently processed cassava roots in a diet deficient in sulfur amino acid have been reported to cause the irreversible paralytic disease konzo, afflicting thousands of women and children in the remote rural areas of bandundu province. "cossettes" (processed cassava roots) purchased in several markets of kinshasa were analyzed for their ...200211982439
population history of manihot esculenta (euphorbiaceae) inferred from nuclear dna sequences.the nature of gene flow in plants -- including the propensity for interspecific introgression -- makes them interesting candidates for phylogeographical analysis. plant phylogeography studies have been limited, however, by the availability of suitable intraspecific variation. in this study, dna sequence variation from a nuclear gene [glyceraldehyde 3-phosphate dehydrogenase; (g3pdh)] was used to examine the population history of manihot esculenta ssp. flabellifolia and a potentially hybridizing ...200211975706
effects of different fibre sources and fat addition on cholesterol and cholesterol-related lipids in blood serum, bile and body tissues of growing pigs.knowledge is limited on the efficacy of hindgut-fermentable dietary fibre to reduce blood, bile and body tissue cholesterol levels. in three experiments with growing pigs the effects of different kinds and levels of bacterially fermentable fibre (bfs) on cholesterol metabolism were examined. various diets calculated to have similar contents of metabolizable energy were supplied for complete fattening periods. in the first experiment, a stepwise increase from 12 to 20% bfs was performed by supple ...200211972674
replacement of maize with cassava sievates in cockerel starter diets: effect on performance and carcass characteristics. 200211969113
solid-state fermentation of phytase from cassava dregs.phytases produced by numerous microorganisms and plants degrade phytic acid that has chelated with metal ions in food and feed. it is important to study phytase for the role of metal ions in nutrition of animals and humans as well as in the reduction of organic phosphate content of aqueous environment. this article reports on solid-state fermentation of phytase from a new substrate of cassava dregs. large quantities of cassava dregs are produced in tropical areas as a byproduct of cassava starch ...200111963905
cassava starch maltodextrinization/monomerization through thermopressurized aqueous phosphoric acid hydrolysis.kinetic conditions were established for the depolymerization of cassava starch for the production of maltodextrins and glucose syrups. thin-layer chromatography and high-performance liquid chromatography analyses corroborated that the proper h3po4 strength and thermopressurization range (e.g., 142-170 degrees c; 2.8-6.8 atm) can be successfully explored for such hydrolytic purposes of native starch granules. because phosphoric acid can be advantageously maintained in the hydrolysate and generate ...200111963875
mutation of three cysteine residues in tomato yellow leaf curl virus-china c2 protein causes dysfunction in pathogenesis and posttranscriptional gene-silencing suppression.the nuclear localized c2 protein of the monopartite begomovirus tomato yellow leaf curl virus-china (tylcv-c) contributes to viral pathogenicity. here, we have investigated tylcv-c c2 protein domains that play a role in the phenotype. alignment of the c2 protein with 67 homologues from monopartite and bipartite begomoviruses revealed that a putative zinc-finger motif c36-x1-c38-x7-c46-x6-h53-x4-h58c59 and four potential phosphorylation sites (t52, s61, y68, and s74) are highly conserved. when ex ...200211952122
effect of food plants on the volume of repellent secretion obtained in adult zonocerus variegatus (orthoptera: pyrgomorphidae).the volume of secretion obtained from adult zonocerus variegatus (orthoptera: pyrgomorphidae) was influenced by the type of food plants. insects fed on leaves of cassava manihot esculenta, bitter leaves vernonia amygdalina, and a mixture of m. esculenta and acalypha wilkesiana gave a good volume of secretion while chromolaena odorata, elaeis guinensis, aspilia africana and citrus sinensis did not favour secretion production. no significant difference was recorded in the volume of secretion obtai ...200111935920
progress in breeding for trace minerals in staple crops.staples are not considered an important source of minerals in the diet. however, because of high staple consumption, any increase in mineral concentration might well have a significant effect on human nutrition and health. the nutritional quality of staple crops (rice, cassava, wheat, maize and beans) can be improved by breeding. studies have shown the potential to exploit the genetic variation in seed concentration of iron and other minerals without the general negative effect on yield of addin ...200211880579
characterisation of sri lankan cassava mosaic virus and indian cassava mosaic virus: evidence for acquisition of a dna b component by a monopartite begomovirus.two bipartite begomoviruses, indian cassava mosaic virus (icmv) and sri lankan cassava mosaic virus (slcmv), have been isolated from mosaic-diseased cassava originating from central india and sri lanka, respectively. icmv was transmitted with low efficiency from cassava to nicotiana benthamiana by sap inoculation to give leaf curl symptoms. slcmv was much more virulent in this host, producing severe stunting, leaf curl, and chlorosis. these symptoms were reproduced when their cloned genomic comp ...200211853400
[an epidemiological study on the risk factors of irritable bowel syndrome in zhanjiang, guangdong province].to assess the risk factors in the pathogenicity of irritable bouel syndrome(ibs) in zhanjiang city.200111851063
exopolysaccharide production from sclerotium glucanicum nrrl 3006 and botryosphaeria rhodina dabac-p82 on raw and hydrolysed starchy materials.evaluation of fermentative usage of raw starchy materials for exopolysaccharide (eps) production by sclerotium glucanicum nrrl 3006 and botryosphaeria rhodina dabac-p82.200211849493
[is manioc a diabetogenic factor? apropos of a case of diabetes mellitus with a large consumption of crude manioc].the case of a 24 year-old comorian male patient consuming large amounts of cooked and uncooked cassava and suffering of malnutrition since his boyhood is reported. the patient presented a diabetes mellitus by chronic calcific pancreatitis with retinopathy and neuropathy. the protein deficiency associated with eating uncooked cassava may be recognised as a factor of calcific pancreatitis diabetes. other factors might be associated such as the environment as well as immunological and genetic chara ...200111845523
new efficient amylase-producing strains of lactobacillus plantarum and l. fermentum isolated from different nigerian traditional fermented foods.amylolytic lactic acid bacteria (alab) were isolated from nigerian traditional fermented foods (fufu, burukutu, ogi-baba and kunu-zakki) with the aim of selecting efficient amylase-producing strains. nine isolates were characterized on the basis of their phenotypic and taxo-molecular characteristics. three groups could be distinguished by their fermentation profiles and this was confirmed by dna restriction analysis. though fermentation profiles gave good identification of strain k9 (unique repr ...200211843413
properties and applications of starch-converting enzymes of the alpha-amylase family.starch is a major storage product of many economically important crops such as wheat, rice, maize, tapioca, and potato. a large-scale starch processing industry has emerged in the last century. in the past decades, we have seen a shift from the acid hydrolysis of starch to the use of starch-converting enzymes in the production of maltodextrin, modified starches, or glucose and fructose syrups. currently, these enzymes comprise about 30% of the world's enzyme production. besides the use in starch ...200211796168
phytate from an alternative dietary supplement has no effect on the calcium, iron and zinc status in undernourished rats.a mixture of cereal bran, eggshells and cassava leaf powder, known as multimixture (mm), has been widely used in developing countries as a dietary supplement to combat malnutrition in children. the introduction of phytate from cereal bran in infant diets has generated serious controversy about mm due to the mineral chelating effect of phytate. this paper reports on a study to investigate the bioavailability of calcium, iron and zinc in rats fed with a deficient diet supplemented with mm. underno ...200111791478
effects of a nonforage diet on milk production, energy, and nitrogen metabolism in dairy goats throughout lactation.the objective of the experiment was to compare a silage-based control diet (c) with a nonforage diet (nf) in dairy goats throughout lactation in terms of animal performance and energy utilization. eight saanen goats were divided into two groups and fed c or nf, a commercial blend that included sunflower meal, cassava, coconut meal, and whole cottonseeds as the main ingredients that was characterized by a small particle size and a high crude protein content. in early, mid, and late lactation (44, ...200111768086
upflow anaerobic sludge blanket treatment of starch wastewater containing cyanide.treatment of tapioca starch wastewater containing cyanide using an upflow anaerobic sludge blanket (uasb) process was investigated. sludge from an anaerobic lagoon treating tapioca starch wastewater was used as seed. performance of the uasb reactor with influent cyanide concentrations up to 25 mg/l was assessed. the inhibitory effects of cyanide were temporary and reversible. the process required longer recovery period for higher cyanide dosage. for 25 mg/l of cyanide concentration in the feed, ...200611765999
differential esterase expression in leaves of manihot esculenta crantz infected with xanthomonas axonopodis pv. manihotis.the polyacrylamide gel electrophoresis system (page) and inhibition tests for biochemical characterization of alpha- and beta-esterases were used to obtain a functional classification of esterases in plants and to show a differential expression of esterases as markers of pathogenesis in cassava plants (manihot esculenta crantz). the characterization of alpha- and beta-esterases from leaves of m. esculenta by the page system was possible using an extraction solution containing two phenol-complexi ...200111758725
structure determinants of substrate specificity of hydroxynitrile lyase from manihot esculenta.tryptophan 128 of hydroxynitrile lyase of manihot esculenta (mehnl) covers a significant part of a hydrophobic channel that gives access to the active site of the enzyme. this residue was therefore substituted in the mutant mehnl-w128a by alanine to study its importance for the substrate specificity of the enzyme. wild-type mehnl and mehnl-w128a showed comparable activity on the natural substrate acetone cyanohydrin (53 and 40 u/mg, respectively). however, the specific activities of mehnl-w128a ...200211742123
water quality of a reservoir as affected by agriculture in the east of thailand: a preliminary study.a preliminary study on the water quality of a reservoir, affected by agriculture, in the east of thailand was conducted during 1996-1997. monitoring water quality of a reservoir is important because the sloping lands surrounding the reservoirs are mainly utilized for cultivating cash crops (pineapple, cassava, etc). a lot of fertilizers and agrochemicals were applied to soil and crops which can polluted the water. the results from the preliminary studied will be applied for the monitoring of the ...200111724479
tropical plant pathology: at home and abroad.i first describe my introduction to plant pathology and early experiences with employment, the environment, diseases, pests, and various plant pathologists. then i recount a decade of stimulating studies at the university of minnesota and the route i followed to a career in international agriculture with the rockefeller foundation in colombia and later at cornell university. my appreciation for and knowledge of traditional farmers and sustainable agriculture occurred as a slow awakening. comment ...200111701856
molecular evidence for diverse populations of cassava-infecting begomoviruses in southern africa.in this paper we describe results obtained from screening field populations of begomoviruses from infected cassava from six countries in southern africa. it was found that african cassava mosaic virus occurs in five of the six countries (except angola), east african cassava mosaic virus is present in five countries (except zambia) and south african cassava mosaic virus was detected only in south africa and swaziland. in addition, we report for the first time in southern africa, the appearance of ...200111699964
the influenza pandemic of 1918-19 and the spread of cassava cultivation on the lower niger: a study in historical linkages. 198111632223
comparison of culture media, simplate, and petrifilm for enumeration of yeasts and molds in food.the efficacy of three culture media, dichloran rose bengal chloramphenicol (drbc), dichloran 18% glycerol agar (dg18), and potato dextrose agar (pda) supplemented with two antibiotics, were compared with the simplate and petrifilm techniques for mold and yeast enumeration. the following foods were analyzed: corn meal, wheat flour, cassava flour, bread crumbs, whole meal, sliced bread, ground peanuts, mozzarella cheese, grated parmesan cheese, cheese rolls, orange juice, pineapple pulp, pineapple ...200111601710
carbon from cassava peel, an agricultural waste, as an adsorbent in the removal of dyes and metal ions from aqueous solution.cassava (manihot esculenta) is a short lived erect perennial shrub, planted vegetatively from hard wood stem cuttings. it is an important crop across a wide range of tropical environments and is a significant component of cropping systems. cassava peel is an agricultural waste from the food processing industry. activated carbons prepared from waste cassava peel employing physical and chemical methods were tested for their efficiency in the removal of dyes and metal ions from aqueous solution. wh ...200111601548
enrichment of eggs with n-3 polyunsaturated fatty acids: effects of vitamin e supplementation.eggs enriched with n-3 polyunsaturated fatty acids (pufa) could contribute to dietary intake of these healthful fatty acids (fa). because n-3 pufa are highly susceptible to peroxidation, a first part of the study with leghorn laying hens was carried out to investigate the influence of different levels of fish oil (0, 0.7, 1.4, 2.8, or 5.6%, respectively) in the diet on n-3 pufa, cholesterol, vitamin e, and lipid peroxidation product contents in eggs. addition of fish oil to a complete diet based ...200111592735
[discovery of phytopathogenic bacteria 100 years ago: transatlantic controversies and polemics].the demonstration of a bacterial cause of some plant diseases has been claimed few years after it was commonly recognized that bacteria were able to cause diseases of human and animal. nevertheless, some sharp controversies took place, between german and american specialists (1897-1901), before the existence of bacterial diseases of plants was accepted by all phytopathologists. nowadays, about 350 bacteria are described, which infect plants: they are pathovars, or subspecies, belonging to 21 gen ...200111570278
identification of a target population for immunisation against east coast fever in coastal kenya.two experiments were carried out to identify the target population of cattle for immunisation against east coast fever (ecf) using the infection-and-treatment method. firstly, a sentinel-calf study was used to determine the age window for ecf immunisation by determining ages at clinical detection of infection with theileria parva. six groups of five naive cross-bred (bos taurus/bos indicus) male calves, introduced at intervals of 2 months at a mean age of 26 days, were exposed to natural tick ch ...200111566376
a longitudinal study of disease incidence and case-fatality risks on small-holder dairy farms in coastal kenya.a longitudinal study was carried out in the coastal lowlands coconut-cassava agro-ecological zone of kaloleni division, coast province, kenya between june 1990 and december 1991 to estimate disease incidence and cause-specific case-fatality risk in an average of 120 cattle in 26 small-holder dairy herds kept in two grazing-management systems. east coast fever (ecf) was the predominant disease diagnosed; the mean monthly incidence rate was 2.5 and 6.9% in animals < or = 18 months of age under sta ...200111566375
seroprevalences of vector-transmitted infections of small-holder dairy cattle in coastal kenya.a cross-sectional study was carried out from july to september 1989 in kaloleni division, coast province, kenya to estimate the prevalence of vector-transmitted diseases in small-holder dairy cattle and to identify the risk factors associated with different management systems. one hundred and thirty of the 157 herds with dairy cattle in kaloleni division were surveyed. these were from three agro-ecological zones (coconut-cassava, cashew nut-cassava and livestock-millet), comprised two management ...200111566374
unmanaged sexual reproduction and the dynamics of genetic diversity of a vegetatively propagated crop plant, cassava (manihot esculenta crantz), in a traditional farming system.occurrence of intervarietal or interspecific natural crosses has been reported for many crop plants in traditional farming systems, underlining the potential importance of this source of genetic exchange for the dynamics of genetic diversity of crop plants. in this study, we use microsatellite loci to investigate the role of volunteer seedlings (plants originating from unmanaged sexual reproduction) in the dynamics of genetic diversity of cassava (manihot esculenta crantz), a vegetatively propag ...200111555234
starch digestion rate in the small intestine of broiler chickens differs among feedstuffs.dietary starch is the major energy source for broiler chickens, and knowledge about its digestive behavior can be important. in a digestibility trial with 720 broiler chickens, site, rate and extent of starch digestion were measured for 12 feedstuffs. starch digestion was determined using the slaughter technique, which involves removal of the small intestine from the recently killed chicken, with manual collection of the contents. starch digestion coefficients were calculated from remaining star ...200111533275
effect of different cultivation conditions on lactobacillus manihotivorans ond32t, an amylolytic lactobacillus isolated from sour starch cassava fermentation.study of the cassava sour starch fermentation has led to the isolation of a new homofermentative amylolytic lactic acid bacterium, lactobacillus manihotivorans ond32t, whose nutritional requirements have been investigated in this work. the main effect of deleting one of the substrate components of the mrs-starch medium was to reduce the amylase production. when starch fermentation with nitrogen as a gas phase was compared to fermentation under aerobic conditions, both growth and amylase producti ...200111518431
[use of diet containing yeast protein (saccharomyces cerevisiae): effects upon pregnancy, lactation and development in rats].the nutritive value of manioc flour (manihot esculenta) enriched with yeast protein (saccharomyces cerevisiae) added to a food mixture most frequently consumed by low-income populations was assessed in female wistar rats (n = 30; 100-120 days old). animals were divided into three groups, mated and had free access to diets and water. diets were as follows: beans, rice, yeast-enriched manioc flour (brymf17); beans, rice, manioc flour (brmf13); casein (17% protein) (cas17). body weight gains and fo ...200111515236
[social exchange and inference: an experimental study with the wason selection task].social contract theory (cosmides, 1989) posits that the human mind was equipped with inference faculty specialized for cheater detection. cosmides (1989) conducted a series of experiments employing the wason selection task to demonstrate that her social contract theory could account for the content effects reported in the literature. the purpose of this study was to investigate the possibility that the results were due to experimental artifacts. in the current experiment, the subject was given t ...200111494657
subcellular targeting of the coat protein of african cassava mosaic geminivirus.the coat protein (cp) of geminiviruses is involved in a number of processes during the life cycle of the virus. the predominant function is encapsidation of single-stranded dna and formation of the virus particle to protect viral dna during transmission. the cp of monopartite geminiviruses is absolutely essential for virus movement, whereas cp mutants of bipartite geminiviruses are able to infect some host plants systemically, indicating an involvement of the cp in host specificity. during the l ...200111485405
screening of cassava and yam cultivars for resistance to anthracnose using toxic metabolites of colletotrichum species.collectotrichum gloeosporioides f. sp. manihotis and c. gloeosporioides, causal agents of cassava (manihot spp.) and yam (dioscorea spp.) anthracnose diseases, respectively, produce toxic metabolites in culture that fluoresce at 254 nm and 366 nm, producing bands with rf of 0.65 and 7.0, respectively. symptoms induced on yam and cassava by the extracted metabolites were similar to those induced by the pathogens. twenty-four clones of tropical d. rotundata (tdr), d. alata (tda), d. esculenta (tde ...200111469761
physiopathological effects of the administration of chronic cyanide to growing goats--a model for ingestion of cyanogenic plants.ingestion of cyanogenic plants, such as cassava and sorghum, has been associated with goitre and tropical pancreatic diabetes in both humans and animals. thus, the objective of the present study was to determine the toxic effects on the thyroid and pancreas in growing goats of prolonged exposure to potassium cyanide (kcn). thirty-four male goats were divided into five groups dosed with kcn at 0 (control). 0.3, 0.6, 1.2 or 3.0 mg/kg daily for 5 months. blood samples were obtained in order to dete ...200111469509
characterization of sucrose uptake system in cassava (manihot esculenta crantz).a leaf disc system was developed to study sucrose uptake in cassava (manihot esculenta crantz). the uptake of (u-(14)c) sucrose by cassava leaf discs followed michaelis-menten kinetics with a k(m) value for sucrose of 1.3 mm. it was found to be strongly inhibited by sulfhydryl reagents, n-ethylmaleimide, p-chloromercuribenzene sulfonate and iodoacetate. several metabolic inhibitors were also tested. among these, dinitrophenol, chlorocarbonyl cyanide phenylhydrazone, phloridzin and vanadate inhib ...200111448748
characteristics and significance of yeasts' involvement in cassava fermentation for 'fufu' production.six different strains of yeast, namely candida krusei, c. tropicalis, pichia saitoi, saccharomyces cerevisiae, p. anomala and zygosaccharomyces bailii were found present in cassava-fermenting water in the early part of the fermentation. the latter part of the fermentation was dominated in all cases by three strains of yeast namely c. krusei, c. tropicalis and z. bailii. all the yeast strains exhibited amylolytic capabilities while none was able to produce cellulase. all the strains except zygosa ...200111393690
microbial ecology of muffins baked from cassava and other nonwheat flours.cassava flour was evaluated as a partial substitute for wheat flour in gluten-free muffins. four replicate test bakes were carried out to evaluate the microbial ecology of two muffin mixes, made with different combinations of cassava, soya, sorghum, sago, and potato flour. the muffins were baked at 180 degrees c for 25 min, stored at 30 degrees c and analysed microbiologically for up to 10 days post-baking. raw materials were similarly analysed microbiologically on the day of baking. characteriz ...200111368089
origin and environmental setting of ancient agriculture in the lowlands of mesoamerica.archaeological research in the gulf coast of tabasco reveals the earliest record of maize cultivation in mexico. the first farmers settled along beach ridges and lagoons of the grijalva river delta. pollen from cultivated zea appears with evidence of forest clearing about 5100 calendar years b.c. (yr b.c.) [6200 (14)c years before the present (yr b.p.)]. large zea sp. pollen, typical of domesticated maize (zea mays), appears about 5000 calendar yr b.c. (6000 yr b.p.). a manihot sp. pollen grain ...200111359011
dietary factors in relation to the distribution of duodenal ulcer in india as assessed by studies in rats.the prevalence of duodenal ulcer is less in the northern wheat-eating regions of india and china than in the southern rice-eating areas.200111350544
expression of the oligomerization domain of the replication-associated protein (rep) of tomato leaf curl new delhi virus interferes with dna accumulation of heterologous geminiviruses.the minimal dna binding domain of the replication-associated protein (rep) of tomato leaf curl new delhi virus was determined by electrophoretic mobility gel shift analysis and co-purification assays. dna binding activity maps to amino acids 1-160 (rep-(1-160)) of the rep protein and overlaps with the protein oligomerization domain. transient expression of rep protein (rep-(1-160)) was found to inhibit homologous viral dna accumulation by 70-86% in tobacco protoplasts and in nicotiana benthamian ...200111342533
[cloning, expression and preliminary application of a alpha-hydroxynitrile lyase from cassave].alpha-hydroxynitrile lyase (me-hnls, e.c. 4.1.2.3.37) from the cyanogenic crop cassava(manihot esculentz, crantz) catalyze the condensation of hydrocyanic acid and aldehydes or ketone into (s)-cyanohydrins, which are valuable starting material for various optically active compounds, such as pharmaceuticals and agrochemicals. the cdna of a me-hnl were obtained by rt-pcr and cloned. the sequencing result for the cdna showed that the sequence encoded for the me-hnl was inconsistent with all those w ...200111330194
amylopectin aggregation as a function of starch phosphate content studied by size exclusion chromatography and on-line refractive index and light scattering.starches with a natural 65-fold span in covalently bound phosphate content were prepared from five different crops including sorghum, cassava, three potato varieties and an exotic ginger plant, curcuma zedoaria, with extreme starch phosphate content. these starches were subjected to size exclusion chromatography with refractive index detection (sec/ri). a simple and rapid method for starch solubilisation was used. the conditions during solubilisation (2 m naoh) and separation (10 mm naoh, 50 deg ...200111325429
simultaneous analysis of the bidirectional african cassava mosaic virus promoter activity using two different luciferase genes.the expression of geminivirus genes is controlled by bidirectional promoters which are located in the large intergenic region of the circular dna genomes and specifically regulated by virus encoded proteins. in order to study the simultaneous regulation of both orientations of the dna a and dna b promoters of african cassava mosaic virus (acmv), they were cloned between two different luciferase genes with the firefly luciferase gene in complementary-sense and the renilla luciferase gene in virio ...200111324760
dynamics of the microbial community responsible for traditional sour cassava starch fermentation studied by denaturing gradient gel electrophoresis and quantitative rrna hybridization.the microbial community developing during the spontaneous fermentation of sour cassava starch was investigated by cultivation-independent methods. denaturing gradient gel electrophoresis (dgge) of partially amplified 16s rdna followed by sequencing of the most intense bands showed that the dominant organisms were all lactic acid bacteria (lab), mainly close relatives of bifidobacterium minimum, lactococcus lactis, streptococcus sp., enterococcus saccharolyticus and lactobacillus plantarum., clos ...200111322700
methanobacterium congolense sp. nov., from a methanogenic fermentation of cassava peel.strain ct, a non-motile, mesophilic, hydrogenotrophic, methanogenic bacterium, was isolated from an anaerobic digester used for the treatment of raw cassava-peel waste in congo. the cells were rods, 0.4-0.5 x 2-10 microm in size, and stained gram-positive. hydrogen and carbon dioxide were the only substrates that supported growth and methane production. methane production, but not growth, occurred with co2 in the presence of either 2-propanol, 2-butanol or cyclopentanol as hydrogen donors. the t ...200111321095
cassava-soy weaning food: biological evaluation and effects on rat organs.weaning food was formulated using a cassava product, 'tapioca' (tap), supplemented with roasted-sprouted soybeans (ss), with and without additional (10%) malted sorghum flour (ms). biological evaluation was carried out on the formulations using 4-5 week old weanling albino rats, with cerelac (a commercial maize-milk weaning food) as the control diet. there were increases in growth rate of rats fed with both the test and control diets. there were no significant (p >0.05) differences between the t ...200111318505
cooperativity and substrate specificity of an alkaline amylase and neopullulanase complex of micrococcus halobius or-1.the saccharifying alkaline amylase and neopullulanase complex of micrococcus halobius or-1 hydrolyzes both alpha-(1,4)- and alpha-(1,6)-glycosidic linkages of different linear and branched polysaccharides. the following observations were made concerning the analysis of the coexpressed amylase and neopullulanase enzymes. even though the enzymes were subjected to a rigorous purification protocol, the activities could not be separated, because both the enzymes were found to migrate in a single peak ...200111318036
mechanistic aspects of cyanogenesis from active-site mutant ser80ala of hydroxynitrile lyase from manihot esculenta in complex with acetone cyanohydrin.the structure and function of hydroxynitrile lyase from manihot esculenta (mehnl) have been analyzed by x-ray crystallography and site-directed mutagenesis. the crystal structure of the mehnl-s80a mutant enzyme has been refined to an r-factor of 18.0% against diffraction data to 2.1-a resolution. the three-dimensional structure of the mehnl-s80a-acetone cyanohydrin complex was determined at 2.2-a resolution and refined to an r-factor of 18.7%. thr11 and cys81 involved in substrate binding have b ...200111316882
does prolonged cyanide exposure have a diabetogenic effect?cyanide exposure through cassava consumption has been associated with the development of malnutrition-related diabetes mellitus (mrdm). however, there are few experimental reproductions of this disease. in the present study 42 rats received 0, 9.0 or 12.0 mg kcn/kg bw/d for 15 d, 26 pigs were dosed with 0, 2.0, 4.0 or 6.0 mg kcn/kg for 74 d, and 34 goats received 0, 0.3, 0.6, 1.2 or 3.0 mg kcn/kg for 5 mo. at the end of each experimental period, plasma samples were obtained for glucose and thioc ...200111308117
expression of a bifunctional green fluorescent protein (gfp) fusion marker under the control of three constitutive promoters and enhanced derivatives in transgenic grape (vitis vinifera).activity of three constitutive promoters and enhanced derivatives in transgenic grape (vitis vinifera l. cv. thompson seedless) was characterized using a bifunctional fusion marker containing the enhanced green fluorescent protein (egfp) and neomycin phosphotransferase (nptii) genes. relative differences in transient gfp expression and stable transformation efficiencies were used to compare promoter activity. expression patterns in transformed somatic embryos revealed that the act2 promoter from ...200111297784
effect of dietary factors on the detection of fecal occult blood in cats.eight different diets were each fed to 6 cats to evaluate the effect on a guaiac and an o-tolidine fecal occult blood test. fecal samples were collected from day 5 through day 7. canine blood or pure cottage cheese were used as positive and negative controls, respectively. one hundred thirty-four fecal samples were analyzed. the dry fish (capelin) and vegetable (tapioca) diet and the pure cottage cheese diet had only negative results in both tests, whereas a canned chicken and cereal (rice) diet ...200111289220
isozyme diversity in cassava cultivars (manihot esculenta crantz).isoenzyme electrophoresis was used as a method to determine genetic diversity in various m. esculenta cultivars collected in the southwestern (sw) and northwestern (nw) regions of the state of parana, in the south region of brazil, and in cultivars produced at the agronomic institute of campinas (iac), são paulo state, southeastern region of brazil. the cultivars have been maintained by vegetative propagation for 5 years and are useful in production programs. a total of 28 loci in the acid phosp ...200011221580
a comparative study of modified starches in direct compression of a water soluble drug-chloroquine phosphate.some in vitro properties of chloroquine phosphate tablets formulated with four modified starches were investigated. the drug was formulated as tablets containing 250 mg of chloroquine phosphate and produced by the direct compression technique. the starches were isolated from maize, zea mays, rice, oryza sativa, cassava, manihot esculenta and cocoyam, zanthosoma sagittifolium. they were modified through physicochemical process, sta-rx 1500, a directly compressible starch was used as basis for com ...201311213430
efficient production of transgenic cassava using negative and positive selection.in order to improve the efficiency of cassava (manihot esculenta crantz) transformation, two different selection systems were assessed, a positive one based on the use of mannose as the selective agent, and a negative one based on hygromycin resistance encoded by an intron-containing hph gene. transgenic plants selected on mannose or hygromycin were regenerated for the first time from embryogenic suspensions cocultivated with agrobacterium. after the initial selection using mannose and hygromyci ...200011206969
effects of dietary fermentable carbohydrates on energy metabolism in group-housed sows.the effect of dietary nonstarch polysaccharide (nsp) content on the metabolic rate in group-housed sows was studied. twelve groups of six nonpregnant sows were each fed one of four experimental diets similar in composition except for the starch and nsp content. exchanging sugar beet pulp silage (sbps) for tapioca created the difference in starch and nsp ratio in the diet. on a dm basis, diets contained 0, 10, 20, or 30% sbps. sows were group-housed and fed at 1.30 times the assumed maintenance e ...200111204695
performance of growing goats fed gliricidia maculata.sixteen female crossbred (localxanglo-nubian) goats raised from 20-30kg were fed a basal diet of cassava chips, cotton seed cake, rice bran and rice straw, where 0, 30, 40 or 50% of the diet dry matter was replaced with leaves from gliricidia maculata. all the diets containing gliricidia resulted in a higher intake than the diet without gliricidia, but the diet with 30% gliricidia gave the best intake and the highest growth rate, 105g per day. total intake was 2.8% of the body weight (bw). five ...200111182303
structure of hydroxynitrile lyase from manihot esculenta in complex with substrates acetone and chloroacetone: implications for the mechanism of cyanogenesis.the crystal structures of hydroxynitrile lyase from manihot esculenta (mehnl) complexed with the native substrate acetone and substrate analogue chloroacetone have been determined and refined at 2.2 a resolution. the substrates are positioned in the active site by hydrogen-bond interactions of the carbonyl o atom with thr11 og, ser80 og and, to a lesser extent, cys81 sg. these studies support a mechanism for cyanogenesis as well as for the stereospecific mehnl-catalyzed formation of (s)-cyanohyd ...200111173464
the size of encapsidated single-stranded dna determines the multiplicity of african cassava mosaic virus particles.transgenic nicotiana benthamiana plants harbouring a defective interfering (di) dna of african cassava mosaic virus (acmv) and control plants were inoculated with acmv. virus particles were purified from infected plants, separated in sucrose gradients and fractions were analysed by southern blotting. transgenic plant-derived virus particles taken from the top fractions of sucrose gradients contained di dna, middle fractions contained a mixture of genomic and di dna and bottom fractions contained ...200111172110
recombination, pseudorecombination and synergism of geminiviruses are determinant keys to the epidemic of severe cassava mosaic disease in uganda.the molecular variability of cassava geminiviruses occurring in uganda was investigated in this study. infected cassava plants and whiteflies were collected from cassava plantings in different geographical areas of the country and pcr was used for molecular characterization of the viruses. two complete sequences of dna-a and -b from african cassava mosaic virus (acmv), two dna-a sequences from east african cassava mosaic virus (eacmv), two dna-b sequences of eacmv and the partial dna-a nucleotid ...200111172108
hydroxypropyl-distarch phosphate from tapioca starch reduces zinc and iron absorption, but not calcium and magnesium absorption, in rats.male rats were fed a fiber-free, purified diet containing either gelatinized tapioca starch that was not modified chemically (ts, 50 g/kg diet) or gelatinized chemically modified tapioca starch (cms, 50 g/kg) for 21 d. ts was used as the control. the six kinds of gelatinized hydroxypropyl distarch phosphate (hdp) from tapioca with two different degrees of substitution (ds) and three different degrees of cross-linking (dc) were used as cms sources. the wet weight and moisture of fecal output of t ...200111160549
microsatellite variation in cassava (manihot esculenta, euphorbiaceae) and its wild relatives: further evidence for a southern amazonian origin of domestication.genetic variation at five microsatellite loci was used to investigate the evolutionary and geographical origins of cassava (manihot esculenta subsp. esculenta) and the population structure of cassava's wild relatives. two hundred and twelve individuals were sampled, representing 20 crop accessions, 27 populations of cassava's closest wild relative (m. esculenta subsp. flabellifolia), and six populations of a potentially hybridizing species (m. pruinosa). seventy-three alleles were observed acros ...200111159133
cloning and characterization of estc from burkholderia gladioli, a novel-type esterase related to plant enzymes.by screening a genomic library of burkholderia gladioli (formerly pseudomonas marginata) for clones exhibiting esterolytic activity, the gene for a novel-type esterase (estc) showing significant homology to plant enzymes could be isolated. high homology was found to two hydroxynitrile lyases originating from hevea brasiliensis (tropical rubber tree) and manihot esculenta (cassava), and to two proteins from oryza sativa (rice) that are specifically induced upon infection by pseudomonas syringae p ...200011152069
identification of essential active-site residues in the cyanogenic beta-glucosidase (linamarase) from cassava (manihot esculenta crantz) by site-directed mutagenesis.the coding sequence of the mature cyanogenic beta-glucosidase (beta-glucoside glucohydrolase, ec 3.2.1.21; linamarase) was cloned into the vector pyx243 modified to contain the suc2 yeast secretion signal sequence and expressed in saccharomyces cerevisiae. the recombinant enzyme is active, glycosylated and showed similar stability to the plant protein. michaelis constants for hydrolysis of the natural substrate, linamarin (k(m)=1.06 mm) and the synthetic p-nitrophenyl beta-d-glucopyranoside (pnp ...200111139381
cassava mosaic virus disease in east africa: a dynamic disease in a changing environment.cassava mosaic disease (cmd), now known to be caused by cassava mosaic geminiviruses (family geminiviridae; genus begomovirus), was first reported in east africa in 1894. epidemics occurred in madagascar and uganda in the 1930s and 1940s, and more localised rapid spread of cmd was observed in parts of coastal tanzania in the 1930s and coastal kenya in the 1970s. during the 1990s, a major regional pandemic of an unusually severe form of cmd has expanded to affect parts of at least five countries, ...200011137168
effects of dietary carbohydrates and buffering capacity on nutrient digestibility and manure characteristics in finishing pigs.a 2 x 3 factorial experiment was conducted with 24 finishing pigs (yorkshire x [finnish landrace x dutch landrace]) to determine the effects of dietary buffering capacity (bc) and carbohydrate sources on apparent total tract digestibility (td), n retention, and manure characteristics. twelve of these pigs were fitted with steered ileo-cecal value cannulas to measure the apparent ileal digestibility (id) of n. experimental variables were two levels of bc (high = 600 meq/kg and low = 530 meq/kg) a ...200011132824
complete nucleotide sequence and host range of south african cassava mosaic virus: further evidence for recombination amongst begomoviruses.complete nucleotide sequences of the dna-a (2800 nt) and dna-b (2760 nt) components of a novel cassava-infecting begomovirus, south african cassava mosaic virus (sacmv), were determined and compared with various new world and old world begomoviruses. sacmv is most closely related to east african cassava mosaic virus (eacmv) in both its dna-a (85% with eacmv-mh and -mk) and -b (90% with eacmv-ug2-mld and eacmv-ug3-svr) components; however, percentage sequence similarities of less than 90% in the ...200111125158
enzyme modification of starch granules: formation and retention of cyclomaltodextrins inside starch granules by reaction of cyclomaltodextrin glucanosyltransferase with solid granules.cyclomaltodextrin glucanosyltransferase (cgtase) was adsorbed into starch granules and allowed to react at 37 degrees c. the reaction was conducted with the granules removed from an aqueous environment, but containing 50% w/w water inside the granule. reaction for 20 h gave a maximum of 1.4%, w/w of cyclodextrins (cds) inside the granule. waxy maize and maize starches gave the highest amounts of cds (1.3 and 1.4%, respectively), with tapioca and amylomaize-7 starches giving about 50% less (0.9 a ...200011093706
hydrogen peroxide and flavan-3-ols in storage roots of cassava (manihot esculenta crantz) during postharvest deterioration.cassava storage roots are an important staple food throughout the lowland humid tropics. however, cassava suffers from a poorly understood storage disorder, known as postharvest physiological deterioration (ppd), which constrains its exploitation. in an attempt to broaden the understanding of ppd, nine different cassava cultivars were analyzed for specific compounds accumulating during the process. the production of hydrogen peroxide (h(2)o(2)) is involved in the early stages of ppd in cassava r ...200011087513
genetic relationships among biotypes of bemisia tabaci (hemiptera: aleyrodidae) based on aflp analysis.genetic similarities between 13 samples belonging to nine reference biotypes and two field populations of bemisia tabaci (gennadius), one field population of b. medinae gómez-menor and another of b. afer priesner & hosny, were evaluated using amplified fragment length polymorphism (aflp) markers. the results indicate that b. tabaci biotypes can be grouped together with a minimum similarity coefficient of 0.32 and separated from the two other species with a similarity coefficient of 0.07. bemisia ...200011082556
cross-species amplification of cassava (manihot esculenta) (euphorbiaceae) microsatellites: allelic polymorphism and degree of relationship.microsatellite amplification was performed on cassava (manihot esculenta) and six other different species (all wild) of the manihot genus. we used ten pairs of microsatellite primers previously developed from cassava, detecting 124 alleles in a sample of 121 accessions of the seven species. the number of alleles per locus ranged from four to 21 alleles, and allelic diversity was greater in the wild species than in cassava. seventy-nine alleles, including unique ones, were detected in the wild sp ...200011080115
starch grains reveal early root crop horticulture in the panamanian tropical forest.native american populations are known to have cultivated a large number of plants and domesticated them for their starch-rich underground organs. suggestions that the likely source of many of these crops, the tropical forest, was an early and influential centre of plant husbandry have long been controversial because the organic remains of roots and tubers are poorly preserved in archaeological sediments from the humid tropics. here we report the occurrence of starch grains identifiable as manioc ...200011057665
the effects of calcium benzoate in diets with or without organic acids on dietary buffering capacity, apparent digestibility, retention of nutrients, and manure characteristics in swine.eight barrows (yorkshire x [finnish landrace x dutch landrace]), initially 30 kg bw, were fitted with ileal cannulas to evaluate the effects of supplementing ca benzoate (2.4%) and organic acids (oa) in the amount of 300 meq acid/kg feed on dietary buffering capacity (bc), apparent digestibility and retention of nutrients, and manure characteristics. swine were allotted in a 2 x 4 factorial arrangement of treatments according to a cyclic (8 x 5) changeover design. two tapioca-corn-soybean meal-b ...200011048928
assessment of genetic variability in a traditional cassava (manihot esculenta crantz) farming system, using aflp markers.despite the urgent need to conserve domesticated plant genetic resources, and developing 'on farm' strategies of conservation, the impact of traditional farming practices and of their interaction with ecological factors on the structure and evolutionary dynamics of the genetic variability of crop populations has been little documented. we assessed the genetic variability of 31 varieties of cassava (m. esculenta crantz) traditionally grown by makushi amerindians from guyana, using aflp markers. w ...200011012725
effect of cassava consumption on open-field behavior and brain neurotransmitters in albino rats.diet exerts a critical influence on human biology and thus studies on the interrelationship of nutrition and behavior continues to be a major and important focus of research in the natural experimental sciences. cassava is known to cause metabolic and neurological derangement on long-term consumption as a staple diet in the tropics. in this article we present the effects of cassava consumption on open-field behavior and catecholamine levels in the hypothalamus of albino rats. cassava consumption ...200010978482
chemical, physical and morphometric properties of peruvian carrot (arracacia xanthorrhiza b.) starch.starch was isolated from peruvian carrot (pc)--or arracacha--(arraccacia xanthorrhiza b.) roots. its chemical, physical, physicochemical and granular structural properties were compared to those of commercial cassava starch. scanning electron microscopy revealed a granular size for pc starch ranging between 4 and 26 microns in diameter, with spherical and truncated-egg shapes. pc and cassava starches were similar regarding gross chemical composition and basic physical characteristics but differe ...199910974715
[xanthan production by xanthomonas campestris in a non-conventional culture medium].among 3 varieties of xanthomonas campestris, the variety ocumo (x. campestris pv. ocumo), showed the greatest capacity for producing xanthan. this bacteria grows appropriately and produces this polysaccharide in a wide diversity of carbohydrate sources. however, this strain does not produce xanthan when the carbohydrate comes from lignocellulosic materials. the glucose syrup favepro was the carbon source that showed the best yield (23 g/l) with the greatest viscosity (7000 cps) of xanthan. the o ...199910974710
a convenient synthesis of optically active 5,5-disubstituted 4-amino- and 4-hydroxy-2(5h)-furanones from (s)-ketone cyanohydrins.(s)-ketone cyanohydrins (s)-2 are accessible by enantioselective hcn addition to ketones 1 by using hydroxynitrile lyase from manihot esculenta ((s)-mehnl) as a biocatalyst. acylation of (s)-2 gave the corresponding (s)-acyloxynitriles (s)-3, which can be cyclized by lhmds to give 5,5-disubstituted (s)-4-amino-2(5h)-furanones (s)-4 and (s)-5. different substituents (h. me, obn, oh) in the 3-position of the furanones were introduced by selecting the appropriate acylating agent, which in the case ...200010961401
effect of some fat replacers on the release of volatile aroma compounds from low-fat meat products.the effect of fat content and carbohydrate fat-replacers on the release of volatile odor compounds from beefburger, salami, and frankfurter has been investigated. the reduction in fat content in any of the three meat products studied resulted in a tendency toward an increase in the quantities of volatiles released in the headspace. tapioca starch and maltodextrin appear to delay the release of certain classes of compounds selectively; for instance, tapioca starch appears to slow the release of s ...200010956136
monosaccharide composition of sweetpotato fiber and cell wall polysaccharides from sweetpotato, cassava, and potato analyzed by the high-performance anion exchange chromatography with pulsed amperometric detection method.the cell wall materials (cwms) from sweetpotato (ipomoea batatas cv. kokei 14), cassava (manihot esculenta), and potato (solanum tuberosum cv. danshaku) and commercial sweetpotato fiber as well as their polysaccharide fractions were analyzed for sugar composition by the high-performance anion exchange chromatography with pulsed amperometric detection (hpaec-pad) method. the separation of arabinose and rhamnose, and xylose and mannose, by this method has been improved using a carbopac pa 10 colum ...200010956132
long-term ingestion of cassava (tapioca) does not produce diabetes or pancreatitis in the rat model.cassava (tapioca, manihot) is consumed as a staple food in some developing countries. the intake of cassava has been linked to several diseases including fibrocalculous pancreatic diabetes (tropical calcific pancreatitis). there are few long-term studies on the effect of cassava ingestion on the pancreas in animal models. this article reports on the long-term (up to 1 yr) effects of cassava in the rat model. we found that cassava did not produce diabetes in the rat even after a year of cassava f ...200010952402
molecular diversity of lactic acid bacteria from cassava sour starch (colombia).lactic acid bacteria and more particularly lactobacilli and leuconostoc, are widely found in a wide variety of traditional fermented foods of tropical countries, made with cereals, tubers, meat or fish. these products represent a source of bacterial diversity that cannot be accurately analysed using classical phenotypic and biochemical tests. in the present work, the identification and the molecular diversity of lactic acid bacteria isolated from cassava sour starch fermentation were assessed by ...200010930082
sale of street food in latin america. the mexican case: joy or jeopardy?have you ever visited a public market in mexico, guatemala, panama, colombia, venezuela, peru or brazil? have you ever stopped to eat delicious mayan-style pork tacos, turnovers filled with corn fungus or squash flower, octopus stuffed crepes, crab, cassava, 'alcapurrias' or grasshoppers with lime juice and chili, agave worms or a 'come back to life' seafood cocktail? if you have not, you have been missing a large part of the mexican, guatemalan, panamanian, colombian, venezuelan, peruvian and b ...200010912387
optimization of the production of aroma compounds by kluyveromyces marxianus in solid-state fermentation using factorial design and response surface methodology.studies were carried out for the production of aroma compounds in solid-state fermentation using factorial design and response surface methodology (rsm) experiments. five agro-industrial residues were evaluated as substrate for cultivating a strain of kluyveromyces marxianus. the results proved the feasibility of using cassava bagasse and giant palm bran (opuntia ficus indica) as substrates to produce fruity aroma compounds by the yeast culture. in order to test the influence of the process para ...200010908866
effect of wainan rogo on body weight and plasma glucose in wistar rats.wainan rogo (wr) is a deep-fat fried dough made from grated fresh cassava. it was found in a cross sectional case-control study to have a risk factor 3.2 times higher in diabetic than non diabetic indigenes of borno state of nigeria. this study examines the chemical composition, and effects of wr on body weight and blood glucose in wistar rats. the study found a higher fat and lower protein composition of the wr diet, and found a higher plasma glucose in the wr fed rats and a more significant we ...200010904938
comparisons of effects of raw and gelatinized sago and tapioca starches on serum and liver lipid concentrations in rats.effects of raw and gelatinized sago and tapioca starches on serum and liver lipid concentrations were compared in rats fed a cholesterol-enriched diet. total serum cholesterol and the atherogenic index in the rats fed raw sago starch showed a statistically significant decrease and tendency to decrease, respectively, when compared with those of rats fed raw tapioca starch. both the serum total cholesterol and atherogenic index in the rats fed gelatinized sago starch showed a tendency to decrease ...200010868347
gene genealogies and population variation in plants.early in the development of plant evolutionary biology, genetic drift, fluctuations in population size, and isolation were identified as critical processes that affect the course of evolution in plant species. attempts to assess these processes in natural populations became possible only with the development of neutral genetic markers in the 1960s. more recently, the application of historically ordered neutral molecular variation (within the conceptual framework of coalescent theory) has allowed ...200010860966
the use of a starter culture in the fermentation of cassava for the production of "kivunde", a traditional tanzanian food product.three cassava fermentation methods (spontaneous fermentation, back-slopping and the use of starter culture) for the production of kivunde, executed in three trials at 30 degrees c, were compared in terms of cyanide level reduction, microbiology and product quality improvement. among the isolates from spontaneously fermented cassava batches, four strains were selected on the basis of their enzymatic activities and acid production. all were identified as lactobacillus plantarum and were used as st ...200010857544
enhancement of selectivity for producing gamma-cyclodextrin.the production of cyclodextrins (cds) by cyclodextrin-glycosyl-transferase (cgtase) from bacillus firmus was studied, with respect to the effect of the source of starch upon cd yield and on the selectivity for producing gamma-cd. cyclodextrin production tests were run for 24 h at 50 degrees c, ph 8.0, and 1 mg/l of cgtase, and substrates were maltodextrin or the starches of rice, potato, cassava, and corn hydrolyzed up to d.e. 10. cornstarch was the best substrate for producing gamma-cd. later, ...200010849849
anaerobic digestion from residue of industrial cassava industrialization with acidogenic and methanogenic physical separation phases.a trial was carried out in a continuous regimen, using a completely stirred tank reactor, at acidogenic phase, and a hybrid reactor (upflow anaerobic sludge blanket + fixed bed) at methanogenic phase at room temperature. the residue to be treated came from a flour and cassava meal industry, and the reactors operated for 300 d with affluent chemical oxygen demand (cod) concentrations of 7500, 9000, 11,000, and 14,000 mg/l. the final results showed a biogas production with a content of 80% methane ...200010849839
study of biocatalyst to produce ethanol from starch. coimmobilization of glucoamylase and yeast in gel.this article presents a detailed study on the conditions for achieving a stable biocatalyst to be used in the production of ethanol from starch. different pellets were used depending on which characteristic of the biocatalyst was being studied: (a) saccharomyces cerevisiae entrapped in pectin or calcium alginate gel particles; (b) silica containing immobilized glucoamylase entrapped in pectin gel particles; or (c) pectin gel particles, with the silica-enzyme derivative and yeast coimmobilized. t ...200010849824
characterization of a granulovirus from the cassava hornworm (erinnyis ello: sphingidae).a colombian isolate of erinnyis ello granulovirus (eegv) was characterized by electron microscopy, restriction endonuclease digestion, and sds-page. electron microscopy showed the occlusion bodies to have a morphology typical of granuloviruses. the restriction patterns of dna from eegv and the granuloviruses of trichoplusia ni (tngv) and pieris rapae (prgv) show little or no similarity, indicating little relatedness among these viruses. eegv was estimated to possess a relatively small genome of ...200010843834
Displaying items 1401 - 1500 of 1944