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the efficacy and safety of spiramycin in the treatment of nongonococcal urethritis in men.twenty-five male patients with nongonococcal urethritis including 15 chlamydial infections, were treated with spiramycin for ten days. all but four patients had been treated previously, mostly with tetracyclines. chlamydia trachomatis was cultured in seven patients and was detected in three additional men by immunofluorescent smear. five other patients had antibodies to chlamydia, and one patient yielded a positive culture for ureaplasma urealyticum and mycoplasma hominis. a successful clinical ...19883053571
conjugal transfer of transposon tn916 from streptococcus faecalis to mycoplasma hominis.transposon tn916 was transferred from streptococcus faecalis to mycoplasma hominis by a mating process resembling conjugation with a frequency of 10(-6) to 10(-7). tn916 was inserted into the mycoplasmal chromosome in single and multiple copies.19873038852
sexually communicable micro-organisms in human semen samples to be used for artificial insemination by donor.two hundred and thirty seven semen samples from 10 institutes for artificial insemination by donor (aid) in belgium and the netherlands were tested for the presence of neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, herpes simplex virus, and cytomegalovirus. the incidence of these micro-organisms in the semen samples was 0%, 6.3%, 4.6%, 35.9%, 0%, and 0.4% respectively, and 47% of all samples were infected with one or more of the micro-organisms. as the ...19873034761
prevalence of genital pathogens among female prostitutes in new york city and in rotterdam.the authors studied the prevalence of genital microorganisms among 300 female prostitutes in brothels in new york city and 60 female prostitutes attending a sexually transmitted diseases clinic in rotterdam, the netherlands. rates of isolation of neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum in the two cities were 9.3% and 8.3%, 25.3% and 16.6%, 57.3% and 74.9%, and 73% and 79%, respectively. trichomonas vaginalis was detected in 3.6% of new york pr ...19863012805
tetracycline-resistant mycoplasma hominis strains contain streptococcal tetm sequences.clinical isolates of mycoplasma hominis resistant to high levels of tetracycline contained dna sequences homologous to the streptococcal tetracycline determinant, tetm. in contrast, none of the susceptible m. hominis isolates tested carried this determinant. this is the first description of tetm in an unrelated genus and suggests the spread of tetm from streptococcus spp. to mycoplasma spp.19852994555
the association of sexually transmitted diseases with cervical intraepithelial neoplasia: a case-control study.thirty-three women with histologically confirmed cervical intraepithelial neoplasia (grades i to iii, with one case of microinvasive carcinoma) and 54 women without evidence of the disease were prospectively studied to determine the relationship of genital infection to cervical neoplasia. demographic and sexual data for patients and control subjects were collected, with standardized clinical and colposcopic evaluation by means of predefined diagnostic categories. cultures from the cervix were ex ...19852982264
in vitro susceptibilities of mycoplasmas and ureaplasmas to new macrolides and aryl-fluoroquinolones.in vitro activities of the new macrolides clarithromycin, previously designated a-56268 (te-031), and a-63075 and of the aryl-fluoroquinolones difloxacin (a-56619) and temafloxacin (a-62254) against 14 strains of mycoplasma pneumoniae, 20 strains of mycoplasma hominis, and 28 strains of ureaplasma urealyticum were compared with that of erythromycin. all three macrolides inhibited growth of m. pneumoniae at less than 0.125 micrograms/ml. no macrolide was active against m. hominis. for five strain ...19882973283
[course of pregnancy and labor and pregnancy outcome for mother and fetus in women infected with mycoplasma hominis]. 19852932010
sociocultural factors in puerperal infectious morbidity among navajo women.from 1980 to 1982, a sample of 968 pregnant navajo women in new mexico was enrolled in a prospective study of biologic and sociocultural factors in puerperal infectious morbidity. past studies have independently implicated both genital infection and psychosocial stressors in perinatal complications, but, to the authors' knowledge, no previous work has concurrently investigated the interactive effects of genital pathogens and psychosocial processes. endocervical cultures for mycoplasma hominis an ...19892916554
case-control study of men with suspected chronic idiopathic prostatitis.we studied prospectively 50 asymptomatic men (24 men from infertile couples and 26 normal volunteers) with no history of genitourinary infection and 34 men referred for symptoms of chronic prostatitis. both groups were evaluated by urethral and prostatic secretion cultures for chlamydia trachomatis, 4-glass prostatic localization cultures for facultative aerobic bacteria (ureaplasma urealyticum, mycoplasma hominis and selected fungi) and counts of prostatic fluid leukocytes. the men with symptom ...19892913355
ureaplasmal pneumonia and sepsis associated with persistent pulmonary hypertension of the newborn.ureaplasma urealyticum was isolated from the lower respiratory tract of three infants with persistent pulmonary hypertension of the newborn. in one, cultures positive for u urealyticum were obtained on multiple occasions from trachea, blood, and pleural fluid prior to the infant's death on postnatal day 6. autopsy findings confirmed the presence of severe pneumonia and the organism was again recovered from multiple sites. a second infant had no apparent predisposing factors for development of pe ...19892909979
sexually transmitted diseases in women with urethral syndrome.frequency of micturition and dysuria were prominent symptoms in 135 (57%) of 237 women with urethral syndrome. ureaplasma urealyticum, mycoplasma hominis and chlamydia trachomatis were the principal organisms associated with the urethral syndrome (38.41%, 28.14% and 11.11%, respectively). escherichia coli was cultured from four patients and herepes genitalis and neisseria gonorrhoea were isolated from five patients. infections with more than one organism were frequent. thirty-one of 135 patients ...19882903082
chronic ureaplasma urealyticum and mycoplasma hominis infections of central nervous system in preterm infants.in a prospective study of meningitis in 100 predominantly preterm infants, ureaplasma urealyticum was isolated from the cerebrospinal fluid (csf) of 8 and mycoplasma hominis from the csf of 5 babies undergoing investigation of suspected sepsis or treatment of hydrocephalus. u urealyticum was isolated from 6 infants with severe intraventricular haemorrhage and from 3 with hydrocephalus. in 4 babies multiple isolations were made over several weeks. there were clinical features of congenital infect ...19882891889
prevalence of chlamydia trachomatis and the genital mycoplasmas in a nonmetropolitan population.results of cultures from patients with nonspecific urethritis (nsu) during a 4-year period were retrospectively analyzed. nsu cultures included chlamydiae and mycoplasmas. seventy-five of 2,408 cultures run by the missoula community hospital microbiology laboratory were positive for chlamydiae (3%). seventy-five of 1,944 mycoplasma cultures were positive for mycoplasma hominis (4%), 532 of 1,944 for ureaplasma urealyticum (27%), and 305 of 1,944, for both (15%), for a total of 46% culture positi ...19872891633
heterogeneity of mycoplasma hominis as detected by a probe for atp genes.use of a plasmid containing part of the atp operon of mycoplasma pg50 as a probe in southern blots show that this region can be used to detect the presence of mycoplasma species in general. dna from 14 different strains of m. hominis was analyzed for restriction fragment length polymorphism (rflp) by the use of the same pg50 probe. the m. hominis strains exhibit considerable polymorphism, although some groupings can be observed. these groupings are compared to those obtained for the same strains ...19872889697
relationship of vaginal ph and papanicolaou smear results to vaginal flora and pregnancy outcome.prematurity is a major cause of perinatal morbidity. studies have implicated components of the vaginal flora in the etiology of some cases of preterm birth. current scoring systems do not include factors which directly reflect the vaginal flora. since papanicolaou smears and the vaginal ph may be affected by the vaginal flora and are easy tests to perform, we studied their relationship to vaginal flora and pregnancy outcome. among 231 patients, those with a vaginal ph greater than or equal to 4. ...19872883041
role of ureaplasma urealyticum and other pathogens in the development of chronic lung disease of prematurity.a prospective cohort study enrolling 107 infants weighing less than 1250 g was conducted between september 1, 1986, and november 15, 1987 in order to determine the role of microorganisms on the development of chronic lung disease (cld). ureaplasma urealyticum was isolated significantly more frequently from gastric aspirates and nasopharyngeal or endotracheal aspirates from 43 infants developing cld than from 56 who did not (51% vs. 16%; p less than 0.005). infants developing cld, defined by radi ...19882845345
in vitro comparison of the activity of doxycycline, tetracycline, erythromycin and a new macrolide, cp 62993, against mycoplasma pneumoniae, mycoplasma hominis and ureaplasma urealyticum.the in vitro activities of doxycycline, tetracycline, erythromycin and a new macrolide, cp 62993, against clinical isolates of mycoplasma pneumoniae (n = 3), mycoplasma hominis (n = 64) and ureaplasma urealyticum (n = 33), were compared using an agar dilution method. m. pneumoniae seemed equally susceptible to the two tetracyclines, while the minimum inhibitory concentrations (mics) of doxycycline were one or two dilution steps lower than the mics of tetracycline for most strains of m. hominis a ...19882843982
elevated antibody titers against cytomegalovirus among patients with testicular cancer.the epidemiology of testicular cancer (tc) has 2 distinguishing features. first, it has an unusual unimodal age-incidence curve which peaks about age 30, and decreases with increasing age. second, risk of the disease has been consistently associated with a high standard of living inasmuch as it is a disease of young white men of generally higher social class in economically developed populations. these features suggest that risk may be related to relatively late age of exposure to common infecti ...19882831159
is genital colonization with mycoplasma hominis or ureaplasma urealyticum associated with prematurity/low birth weight? 19892797647
comparative in vitro activity of lomefloxacin, a difluoro-quinolone.lomefloxacin is a new difluoro-quinolone. in this study, we have determined the in vitro activity of lomefloxacin against a wide range of clinical bacterial isolates and compared it with that of other fluoro-quinolones and some unrelated antimicrobials. lomefloxacin was very active against enterobacteriaceae (mic90, 0.5 micrograms/ml) with activity comparable to that of ofloxacin (mic90, 0.25 micrograms/ml). lomefloxacin was moderately active against isolates of pseudomonas aeruginosa (mic90, 4 ...19892791500
evidence relating bacterial vaginosis to intraamniotic infection.we performed a two-part study to determine relationships of bacterial vaginosis and intraamniotic infection. in the first part of the study, we determined whether bacterial vaginosis organisms (gardnerella vaginalis, mycoplasma hominis, and anaerobes) were associated with each other in the amniotic fluid of 408 cases of intraamniotic infection. in the second part, we determined the association of bacterial vaginosis itself with intraamniotic infection in 125 cases at high risk for intraamniotic ...19892782365
successful management of mycoplasma hominis septic arthritis involving a cementless prosthesis.prompt identification of the infectious agent and antibiotic treatment are essential to the prevention of mortality or serious morbidity in patients with septic arthritis. of concern is the increasing incidence of mycoplasma hominis saprophytes as a cause of joint infections given the problems in isolating these microbes. the case of a 32-year-old black woman with a 9-year history of systemic lupus erythematous who presented with an m hominis-related septic arthritis involving hip and knee joi ...19892773970
neonatal serologic response at term to the genital mycoplasmas.genital mycoplasmas are frequently found in the amniotic fluid (af) of women with ruptured membranes but are infrequent pathogens in the neonates born to these women. the serologic response to the genital mycoplasmas, mycoplasma hominis and ureaplasma urealyticum, was studied in 35 mother-baby pairs following term deliveries. amniotic fluid and neonatal surface cultures were obtained in all cases, as were maternal and neonatal acute and convalescent sera. despite significant maternal serologic r ...19892771528
transmission of genital mycoplasmas from mother to neonate in women with prolonged membrane rupture.colonization of the neonate with genital mycoplasmas occurs during passage through a colonized birth canal or in utero via contamination of the amniotic fluid. to define further the route of transmission we obtained cultures from the maternal vagina, the amniotic fluid and the neonatal pharynx in 131 mother-baby pairs. sixty-six percent (33 of 50) of the corresponding amniotic fluids were colonized when the vagina was colonized with mycoplasma hominis. when the amniotic fluid contained m. homini ...19892771527
incidence of mycoplasma hominis and ureaplasma urealyticum infection in pregnant women and gynaecological patients; the effectivity of doxycycline therapy.the authors report on the results of mycoplasma hominis and ureaplasma urealyticum cultivations performed within two and a half years in 1,421 pregnant women and gynaecological patients. considering the obstetrical-gynaecological patients mycoplasma hominis was identified in 7.25% and ureaplasma urealyticum in 38.11% of cases. no significant difference was found in the incidence of mycoplasma infection when comparing the results of cultivations of samples obtained from a clinically non-inflamed ...19892756511
characterization of a strain of mycoplasma hominis lacking 120 kda membrane protein isolated from vero cell culture.a strain of mycoplasma hominis lacking a major membrane protein of 120 kda was isolated from a vero cell culture. this strain showed very slow growth rate and formed nipple-less colonies on agar medium.19892755364
[genital infections in adolescence].in 126 patients with relapsing cervicitis or abnormal cytologic findings, aged 13-19 years (12.5% were virgins), the most frequently bacteria were ureaplasma urealyticum (30.2%), mycoplasma hominis, and gardnerella vaginalis (14.3%), and chlamydia trachomatis (9.5%). in patients with abnormal cytologic findings, ureaplasma urealyticum was isolated most frequently--5 times in group iiia after papanicolaou and 4 times in group iiib, followed by mycoplasma hominis 3 times in group iiia and iiib eac ...19892739437
prosthetic valve endocarditis caused by mycoplasma hominis. 19892729342
association of chlamydia trachomatis and mycoplasma hominis with intrauterine growth retardation and preterm delivery. the john hopkins study of cervicitis and adverse pregnancy outcome.the authors conducted a prospective study of risk factors for intrauterine growth retardation (birth weight less than a standard (race- and sex-adjusted) 10th percentile for gestational age) and preterm birth (birth prior to 37 weeks gestation) in a high-risk, inner-city, obstetric population, with special interest in pathogens colonizing the maternal genital tract. a total of 801 women within 22 to 30 weeks of gestation were enrolled and interviewed. subjects were cultured for gardnerella vagin ...19892729260
[changes in the biological properties of mycoplasma hominis under the influence of prednisolone and cyclophosphane].the results of experimental investigations on the influence of prednisolone and cyclophosphane on biological properties of m. hominis are presented. changes in the morphology of mycoplasmal colonies depending on the type and dose of the preparation were stated. the virulence of mycoplasmas was found to be intensified with an increase of the concentration of prednisolone in the culture medium from 0.1 to 20.0 micrograms/ml and that of cyclophosphane from 0.2 to 20.0 micrograms/ml.19892725356
susceptibilities of genital mycoplasmas to the newer quinolones as determined by the agar dilution method.the increasing resistance of genital mycoplasmas to tetracycline poses a problem because tetracycline is one of the few antimicrobial agents active against mycoplasma hominis, ureaplasma urealyticum, chlamydiae, gonococci, and other agents of genitourinary-tract disease. since the quinolones are a promising group of antimicrobial agents, the susceptibilities of m. hominis and u. urealyticum to the newer 6-fluoroquinolones were determined by the agar dilution method. ciprofloxacin, difloxacin, an ...19892712541
mycoplasma hominis as a respiratory pathogen in x-linked hypogammaglobulinaemia.we report a case of lower respiratory tract infection with mycoplasma hominis in a patient with x-linked hypogammaglobulinaemia.19892708833
morphological lesions of the rat urinary tract induced by inoculation of mycoplasmas and other urinary tract pathogens.the effects on the urinary tract after inoculation of ureaplasma urealyticum into the rat bladder were evaluated and compared to that seen after mycoplasma hominis, escherichia coli and proteus mirabilis inoculation. the inoculation of the urease-producing organisms p. mirabilis and u. urealyticum were associated with the formation of struvite bladder stones and predominantly hyperplastic lesions of the bladder. the p. mirabilis inoculated rats also displayed marked pyelonephritis. a similar but ...19892678669
infection and labor. v. prevalence, microbiology, and clinical significance of intraamniotic infection in women with preterm labor and intact membranes.amniotic fluid was retrieved by amniocentesis from 264 patients with preterm labor and intact membranes admitted to yale-new haven hospital from jan. 1, 1985, to july 31, 1988. the prevalence of a positive amniotic fluid culture was 9.1% (24/264). a total of 111 patients (42%) delivered preterm neonates, and 24 (21.6%) of those had positive amniotic fluid cultures. the diagnostic indexes of the gram stain of amniotic fluid in the prediction of a positive amniotic fluid culture were as follows: s ...19892675611
detection of chlamydia trachomatis, mycoplasma hominis and ureaplasma urealyticum in pregnant dutch women.a prospective study was performed to determine the prevalence of endocervical infection by chlamydia trachomatis and vaginal colonization by mycoplasma hominis and ureaplasma urealyticum in pregnant women seeking routine obstetrical care in two clinics in the southern part of the netherlands. c. trachomatis was detected using the direct immunofluorescence staining technique. for the genital mycoplasmata, generally accepted culture methods were used. evaluable samples were obtained from 691 of 77 ...19892673884
mycoplasma hominis septic thrombophlebitis in a patient with multiple trauma: a case report and literature review.mycoplasma hominis was recovered from the site of a septic thrombophlebitis on the left cephalic veins of a patient with pelvic and other multiple trauma. the organisms were initially isolated from routine cultures in conventional blood agar media incubated anaerobically. the absence of other demonstrable pathogens and the patient's serologic response to the isolate support the role of the organism as the cause of this previously unreported mycoplasmal infection. m. hominis should be considered ...19892666016
chlamydiazyme test for rapid detection of chlamydia trachomatis.endocervical (120) and endourethral (104) swabs collected from patients attending the gynaecological opd and std clinic of a hospital, in north india were subjected to the chlamydiazyme test to detect c. trachomatis antigen. this antigen was detected in 25 per cent (30 of 120) of cervical and 20.19 per cent (21 of 104) of urethral specimens. of the 51 antigen positive cases, 30 (58.8%) presented with the clinical picture of cervicitis, 11 (21.5%) with urethritis, 6 (11.7%) were cases of primary ...19892661422
is genital colonization with mycoplasma hominis or ureaplasma urealyticum associated with prematurity/low birth weight?mycoplasma species have been implicated in the pathogenesis of prematurity, intrauterine growth retardation, low birth weight (lbw), and preterm premature rupture of membranes. the purpose of this study was to review the available literature to determine whether there is an association between genital colonization with mycoplasma hominis or ureaplasma urealyticum and prematurity/lbw. twelve studies were reviewed: nine cohort studies, two case-control studies, and one randomized clinical trial of ...19892644604
[the significance of ureaplasma urealyticum and mycoplasma hominis in non-specific urethritis].184 urethral swabs from patients with unspecific urethritis were taken. ureaplasma urealyticum was isolated with 86 (46.74%), and mycoplasma hominis with 26 (14.13%) patients. in the control group of 156 examinees u. urealyticum was isolated in 43 (27.56%), and m. hominis in 13 (8.33%) cases. u. urealyticum with patients was isolated in a significantly larger number than with the control group (p less than 0.05) which shows possible part of this mycoplasma in the etiology of unspecific urethriti ...19892640277
oestradiol-induced infection of the genital tract of female mice by mycoplasma hominis.treatment of female balb/c mice with oestradiol rendered them susceptible to vaginal colonization by three of four different strains of mycoplasma hominis. overall, the organisms were recovered persistently from the vagina of 68 (87%) of 78 of these mice. strain to mice given one of the strains were at least susceptible, all of ten becoming colonized and larger numbers of organisms being recovered. the hormone arrested the reproductive cycle in the oestrous phase, characterized by non-nucleated, ...19892632670
[isolation of mycoplasmas from female genitalia].the author tested ways of collection, transport and storage of material for mycoplasmatological examination. the use of tampons on a stick during transport in urea substrate medium proved useful. the site of maximum occurrence of mycoplasmas was the posterior vaginal vault. on examination of the vaginal secretion of 804 women mycoplasma hominis was isolated in 29.6% and ureaplasma urealyticum in 65.2% of the cases. concurrent isolation of mycoplasma hominis and ureaplasma urealyticum was recorde ...19892632013
mycoplasma hominis and ureaplasma urealyticum. comparison of media for isolation and prevalence among patients with nongonococcal nonchlamydial genital infections.one hundred and twelve genital specimens were cultured for mycoplasma hominis and ureaplasma urealyticum using a conventional system and the mycotrim gu biphasic culture system. both systems gave the same percentage of recovery (60%) of mycoplasma hominis, while the recovery rates of ureaplasma urealyticum were 95% with the conventional system and 83% with the mycotrim gu. the media contamination was similar for both systems. the average time for mycoplasma isolation and identification was short ...19892628275
[detection of mycoplasma hominis and ureaplasma urealyticum in patients with leukospermia].cytological and microbiological tests were applied to urethral and prostate secretion, initial, midstream, and postmassage urine, and ejaculate of 61 patients with granulocyte counts of approximately 2 million/ml sperm, using the working principle of four-tube testing. findings included 8 cases of chronic urethritis (caused by chlamydia trachomatis in 5 instances, ureaplasma (u.) urealyticum in 2, and mycoplasma (m.) hominis in 1), 11 cases of chronic bacterial prostatitis, most of them in conco ...19892619480
[the capacity of ureaplasma urealyticum, mycoplasma hominis and seven other mycoplasma species for hemadsorption, sperm adsorption, hemolysis and peroxide formation].searching for potential virulence markers of ureaplasma (u.) urealyticum and mycoplasma (m.) hominis, simple laboratory methods were used to detect strain specific properties. haemadsorption, sperm adsorption, haemolysis, and peroxide formation were tested on clinical isolates and type strains of u. urealyticum and m. hominis in comparison to reference strains of seven other mycoplasma species. in contrast to m. bovis, m. gallisepticum and m. pulmonis, all strains of m. hominis and u. urealyticu ...19892619479
[a preclinical and clinical study of cefmenoxime in newborns].1. cefmenoxime (cmx) was administered with a dosage regimen of 20-25 mg/kg, 2-3 times daily (40-75 mg/kg/day) by intravenous drip over 30 minutes to 9 neonates with bacterial infections including purulent meningitis and septicemia. clinical responses to the treatment were excellent in 7 and poor in 2. bacteriological responses were "eradication of pathogens" from 8 of them except another patient with an infection due to staphylococcus aureus. 2. adverse reactions to cmx were observed in 6 of 18 ...19892614917
[the clinical characteristics of diseases caused by mycoplasma hominis].of 1125 patients with acute respiratory diseases (complicated) and uncomplicated 49 (4.4%) showed infection caused by mycoplasma hominis. the diagnosis was established by serological and microbiological methods. the authors describe the microbiological method of detection of the pathogen in nasopharyngeal wash offs and sputum. illustrative clinical examples are presented.19892609572
septic osteomyelitis and polyarthritis with ureaplasma in hypogammaglobulinemia.we describe a hypogammaglobulinemic woman with a one-year history of destructive septic osteomyelitis and polyarthritis with positive cultures for ureaplasma urealyticum from joint exudate and blood. the clinical course was complicated by subcutaneous abscesses from which both u. urealyticum and mycoplasma hominis were grown. multiple routine cultures had been negative, except for sporadic findings of staphylococcus epidermidis before specific cultures for mycoplasmas were performed. therapy wit ...19892599651
a phylogenetic analysis of the mycoplasmas: basis for their classification.small-subunit rrna sequences were determined for almost 50 species of mycoplasmas and their walled relatives, providing the basis for a phylogenetic systematic analysis of these organisms. five groups of mycoplasmas per se were recognized (provisional names are given): the hominis group (which included species such as mycoplasma hominis, mycoplasma lipophilum, mycoplasma pulmonis, and mycoplasma neurolyticum), the pneumoniae group (which included species such as mycoplasma pneumoniae and mycopla ...19892592342
efficacy of single-agent therapy for the treatment of acute pelvic inflammatory disease with ciprofloxacin.a prospective, randomized, controlled, non-blind clinical trial was conducted to compare the efficacy of monotherapy with ciprofloxacin with that of a combination of clindamycin plus gentamicin in the treatment of patients with acute pelvic inflammatory disease. pretreatment and post-treatment cervical culture specimens were obtained for neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum. pretreatment and post-treatment endometrial culture specimens were ...19892589358
the susceptibility of germ-free, oestradiol-treated, mice to mycoplasma hominis.conventionally reared female balb/c mice, rendered susceptible to mycoplasma hominis infection of the genital tract by treatment with oestradiol, have increased numbers of endogenous vaginal bacteria. the latter was reflected by the occurrence of bacterial growth in 95 (65.5%) of 145 cultures undertaken to isolate m. hominis from oestradiol-treated mice, but in only seven (4.8%) of 146 cultures from untreated animals. in addition, larger numbers of bacteria were seen in vaginal smears from oestr ...19892585474
morphological response of mycoplasma to fleroxacin.quinolones exhibit partially reduced activity in vitro against genital mycoplasma. therefore, the morphological response to fleroxacin, a difluorinated quinolone, by mycoplasma hominis was studied. even at subinhibitory concentrations the ultrastructural investigations revealed distinct alterations such as cytolysis, vacuole and cell "ghost" formation. the typical dumbbell-shaped cells observed with monofluorinated compounds were not found, which may indicate a qualitatively different mode of ac ...19892515157
in vitro activity of lomefloxacin against chlamydia trachomatis, neisseria gonorrhoeae, haemophilus ducreyi, mycoplasma hominis, and ureaplasma urealyticum.the in vitro activity of lomefloxacin, a new difluorinated quinolone antimicrobial was compared to comparative agents against organisms causing sexually transmitted diseases. against chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, neisseria gonorrhoeae, and haemophilus ducreyi, lomefloxacin exhibited mic90 of 2.0, 8.0, 8.0, less than or equal to 0.015, and 0.003 micrograms/ml, respectively. overall, the lomefloxacin activity was very similar to ciprofloxacin.19892507222
sexually transmitted pathogens in acute pelvic inflammatory disease.the prevalence of sexually transmitted pathogens in two groups of women was studied: 50 women with clinical diagnoses of acute pelvic inflammatory disease (pid) and 50 asymptomatic women attending a family planning clinic (fpc). genital specimens, collected by non-invasive procedures, were examined. endocervical neisseria gonorrhoeae was present in 62% of the pid group and 10% of the fpc group (p less than 0.0001). one-third of n. gonorrhoeae isolates were penicillinase-producing strains. chlamy ...19892506653
[diagnosis of sexually transmitted diseases in chilean pregnant adolescents].we searched in 100 healthy pregnant women by isolation, the presence of neisseria gonorrhoeae, herpes simplex, mycoplasma hominis, ureaplasma urealyticum and chlamydia trachomatis. blood was also taken for examination of specific antibodies to these microorganisms. we studied only for antibodies titled cytomegalovirus (cmv), treponema pallidum and human immunodeficiency virus, and condyloma acuminatum by cervical cytology. in 85 adolescents we found 5 (6%) patients with c. trachomatis, four of t ...19892490169
comparison of methods for diagnosing bacterial vaginosis among pregnant women.the diagnosis of bacterial vaginosis is usually based on clinical criteria including homogeneous vaginal discharge, an elevated vaginal ph, the presence of clue cells, and an amine odor. we have evaluated the vaginal flora and clinical signs for 593 pregnant women. gardnerella vaginalis, bacteroides spp., and mycoplasma hominis were isolated more frequently among women with clinical signs than among those without clinical signs of bacterial vaginosis in multivariable analyses that controlled for ...19892473992
[isolation and use of a mycoplasma hominis antigen in the laboratory diagnosis of respiratory diseases]. 19872471038
diagnosis of chlamydia trachomatis infection--culture versus serology.the diagnostic value of different laboratory methods in detecting chlamydia trachomatis infections in high risk groups was analysed. the efficiency of a direct specimen test was compared with serology (igg and igm elisa) and culture in l929 cells, stained either with fluorescein conjugated monoclonal antibodies or with iodine. patients (no. = 1041) with localized genital infections attending a std clinic, sexual contacts and patients with ascending infections from urological and gynecological cl ...19882451616
genomic and gene variation in mycoplasma hominis strains.dnas from 14 strains of mycoplasma hominis isolated from various habitats, including strain pg21, were analyzed for genomic heterogeneity. dna-dna filter hybridization values were from 51 to 91%. restriction endonuclease digestion patterns, analyzed by agarose gel electrophoresis, revealed no identity or cluster formation between strains. variation within m. hominis rrna genes was analyzed by southern hybridization of ecori-cleaved dna hybridized with a cloned fragment of the rrna gene from the ...19872444564
human mycoplasmal infections: serologic observations.three species of mycoplasmas have been implicated in human disease. mycoplasma pneumoniae, which causes upper respiratory infections, pneumonia, and other complications, is an uncommon isolate from the ororespiratory tract except during outbreaks of disease. mycoplasma hominis and ureaplasma urealyticum are the two principal species of genital mycoplasmas. these are responsible for some cases of urogenital infections, and more recent studies show that they are associated with perinatal disorders ...19852408311
a clinical and microbiologic analysis of risk factors for puerperal endometritis.predictors of postpartum endometritis were identified in 607 asymptomatic, laboring women. one hundred (16.5%) developed postpartum endometritis. multivariate analysis using stepwise logistic regression identified cesarean delivery (relative risk 12.8; p less than .0001) as the dominant overall predictor. in patients with cesarean delivery (n = 124), prophylactic antibiotics (relative risk 0.54; p less than .0002) and high-virulence bacteria or mycoplasma hominis (relative risk 1.4; p less than ...19902406660
infection and labor. vi. prevalence, microbiology, and clinical significance of intraamniotic infection in twin gestations with preterm labor.the purpose of this study was to establish the prevalence, microbiology, and outcome of microbial invasion of the amniotic cavity in twin gestation presenting with preterm labor and intact membranes. amniocenteses were performed on both sacs of 46 women with twin gestations, preterm labor, and intact membranes. indigo carmine was injected to ensure sampling of both amniotic sacs. amniotic fluid was cultured for aerobic and anaerobic bacteria, mycoplasma hominis, and ureaplasma urealyticum. a pos ...19902403156
microbiologic efficacy of intravaginal clindamycin cream for the treatment of bacterial vaginosis.bacterial vaginosis is characterized by replacement of the normal lactobacillus-predominant vaginal flora with gardnerella vaginalis, anaerobic bacteria, and mycoplasma hominis. the present study evaluated the vaginal flora of women with bacterial vaginosis before and after treatment with intravaginal clindamycin cream. sixty-seven nonpregnant women with symptoms and signs of bacterial vaginosis, and without other genital tract infections, were randomly assigned to receive placebo cream or 0.1, ...19902381617
polymicrobial infections of the female low genital tract.a complete microbiologic analysis of nine infectious agents causing low genital tract infection (lgti) was obtained in 240 young women, of whom 193 were symptomatic for lgti and 47 were asymptomatic (control group). the polymicrobial nature of lgti was demonstrated by both the increased frequency of different microorganisms (1.77 +/- 1.09 sd in symptomatic vs. 1.08 +/- 0.98 in asymptomatic patients) and the increased total number of agents (0 agents 8.3 and 36.2%, 1-2 agents 67.4 and 55.3%, grea ...19902380032
urethral infection of male monkeys by mycoplasma hominis and ureaplasma urealyticum.seven male macaca nemestrina, eight male macaca mulatta and three male papio hamadrias were infected with m. hominis or u. urealyticum separately or simultaneously into the urethra. animals of the first two species exhibited moderate infection and mild clinical urethritis characterized by increased number of pmnl in the urethral discharge and colonization and lymphocytic infiltration of the mucous membrane of the urethra and urinary bladder. animals of the third species proved to be less sensiti ...19902363322
near fatal puerperal fever due to mycoplasma hominis.we describe a case of severe septicaemia caused by mycoplasma hominis in a 23 year old patient following childbirth. she developed disseminated intravascular coagulation and acute respiratory distress syndrome which have not hitherto been described in association with septicaemia due to this organism. investigation and treatment leading to full recovery is outlined.19902349190
mycoplasma hominis pneumonia and pleural effusion in a postpartum adolescent. 19902336318
mycoplasmal infections of cerebrospinal fluid in newborn infants from a community hospital population.mycoplasma hominis or ureaplasma urealyticum have previously been isolated from cerebrospinal fluid (csf) in 13 of 100 newborn infants tested from a high risk university hospital population where the mothers were of predominantly lower income and socioeconomic status and had often received little or no prenatal care. we sought to determine whether such infections occur in neonates born to women cared for mainly through private obstetric practices and who delivered in 4 suburban community hospita ...19902336309
human ureaplasmas show diverse genome sizes by pulsed-field electrophoresis.contour clamped homogeneous field (chef) agarose gel electrophoresis (age), ramped to give linear separation of dna molecules of 600-1600 kilobase pairs (kbp), was used to determine mobilities for full-sized genomic dna of the serotype standard strains of the human genital mollicutes, ureaplasma urealyticum relative to yeast chromosomal dna markers. indicated genome sizes (in kbp) were 760 for the four biotype 1 strains and 840-1140 for eleven biotype 2 strains. other estimates were: 720 for myc ...19902326188
heart transplantation for intractable prosthetic valve endocarditis.a 25-year-old woman with active systemic lupus erythematosus and infective endocarditis was seen initially with porcine aortic bioprosthetic stenosis, perivalvar regurgitation, and native mitral regurgitation 9 years after aortic valve replacement for lupus endocarditis. double-valve replacement was performed with st. jude medical mechanical prostheses. after operation the patient developed fever and an elevated white blood cell count. one month later she had increasing mitral and aortic perival ...19902319373
common sulfoglycolipid receptor for mycoplasmas involved in animal and human infertility.sulfoglycolipids are ubiquitous components of the male germ cell membrane. sulfogalactoglycerolipid (sgg) is restricted to mammalian cells and has recently been implicated in sperm/egg interactions. mycoplasma infections have been implicated in infertility in a variety of species, including humans. four such species-specific mycoplasmas, ureaplasma urealyticum and mycoplasma hominis (humans), mycoplasma pulmonis (rodents), and ureaplasma diversum (cattle) are not shown to specifically recognize ...19902289018
wound infections after cesarean section with mycoplasma hominis and ureaplasma urealyticum. a report of three cases.mycoplasma hominis and ureaplasma urealyticum were isolated from the surgical wounds of three patients who developed endometritis and a wound infection after cesarean section. in all patients, aspiration of the incision yielded a cloudy serosanguinous exudate. gram stain of the fluid revealed numerous white blood cells but no bacteria. all patients responded to antibiotic therapy and local wound care.19902282776
differences in accumulation of radiolabeled amino acids and polyamines by mycoplasma and acholeplasma species.four species in the order mycoplasmatales, mycoplasma capricolum, mycoplasma hominis, mycoplasma arginini, and acholeplasma laidlawii, were compared for their ability to accumulate radiolabeled amino acids and polyamines. the use of a novel high-molecular-weight (hmw) medium, from which molecules of less than 12,000 molecular weight had been removed by extensive dialysis, allowed us to discern significant differences among the species in their relative accumulations of [3h]methionine and [3h]leu ...19902275861
mycoplasma hominis infections in female genital tract & use of immunofluorescence for antibody detection.a total of 325 women with genital tract infection and 108 healthy controls were screened for the presence of mycoplasmas. of these, 325 patients, mycoplasmas were recovered in 186. thirty five isolates were m. hominis and 151 isolates of ureaplasma urealyticum. the fluorescent-antibody technique (fat) has been employed for the rapid identification of mycoplasma colonies growing on agar plates. the method was found to be rapid for detection of m. hominis growing on primary isolation plates. the a ...19902269508
[microbial flora in semen of infertile african men at garankuwa hospital].this study was undertaken to determine the frequency of bacteria and mycoplasmas in semen of infertile african men and to compare abnormal semen parameters with the presence of these organisms. routine semen analysis were performed on 100 patients, and semen and first-voided urine samples were cultured. mycoplasma hominis was cultured in 28% of seminal fluid and ureaplasma urealyticum in 42%. these organisms were both present in 22% of cultures. bacteriological cultures were positive in 6 urine ...19902264614
cervicovaginal microflora and pregnancy outcome: results of a double-blind, placebo-controlled trial of erythromycin treatment.available information suggests that some instances of preterm birth or premature rupture of membranes are associated with clinically unrecognized infection and inflammation of the lower uterine segment, decidua, and fetal membranes. various cervicovaginal microorganisms have been recovered from these sites. many of these microorganisms produce factors that may lead to weakening of the fetal membranes, release of prostaglandins, or both. this study evaluated the presence of various lower genital ...19902240110
antenatal microbiologic and maternal risk factors associated with prematurity.in a prospective study of 202 women (gestational age 24 +/- 4 weeks), we evaluated possible influences of lower genital tract infection or bacterial conditions on obstetric outcomes, including preterm labor, preterm premature rupture of membranes, and preterm birth. the presence of bacterial vaginosis (18.7%) was associated with an increased risk of preterm labor (relative risk, 2.6; 95% confidence interval, 1.08 to 6.46). for women with bacterial vaginosis who also had mobiluncus species morpho ...19902240089
reservoir of four organisms associated with bacterial vaginosis suggests lack of sexual transmission.this study consisted of a search for the possible reservoir and mode of spread of the four bacterial vaginosis-associated organisms mobiluncus mulieris, mobiluncus curtisii, mycoplasma hominis, and gardnerella vaginalis. their occurrence in rectal, oral, and pharyngeal specimens from women with and without bacterial vaginosis, their male sexual consorts, four homosexual men, and children (altogether, 374 people) was studied. genital samples were also obtained from all adults. all four organisms ...19902229386
enzyme linked immunosorbent assay for immunoglobulins g to mycoplasma hominis.sera (187) from women patients attending the std, obstetrics and gynaecology, and family planning clinics were screened for the presence of the igg class of immunoglobulins against m. hominis, using the elisa technique. sonicates of locally isolated m. hominis serotype (cs1) and standard pg 21 strain were used as antigens. the test was standardized using penicillinase as an enzyme. the elisa showed 90.48 per cent sensitivity and 84.8 per cent specificity, and was also rapid (as compared to cultu ...19902228057
occurrence of ureaplasma urealyticus and mycoplasma hominis in non-gonococcal urethritis before and after treatment in a double-blind trial of ofloxacin versus erythromycin.the efficacy of ofloxacin, a new quinolone derivate, was tested against that of erythromycin in a prospective double-blind trial in patients with non-gonococcal urethritis (ngu) with special reference to the occurrence of mycoplasma hominis and ureaplasma urealyticum. 188 male ngu patients were randomized to treatment with either ofloxacin 200 mg b.i.d. or erythromycin 500 mg b.i.d. for seven days. before treatment eight (4.3%) patients, five in the erythromycin group and three in the ofloxacin ...19902218419
extragenital mycoplasma hominis infections in adults.purpose: to heighten awareness of the role of mycoplasma hominis as an extragenital pathogen in adults. patients and methods and results: patients ranged in age from 14 to 76 years. thirteen patients were immunosuppressed, including nine organ transplant recipients; three were receiving steroids, and two had an underlying malignancy. the remainder were immunocompetent. thirteen patients had prior surgery at or near the site of infection. m. hominis was isolated from normally sterile sites such a ...19902203260
[epidemiology of vaginal mycoplasmas].the authors made in 456 women mycoplasmatological examinations of the vaginal secretion by cultivation. mycoplasma hominis was obtained in 158 women (34.6%). u. urealyticum in 295 women (64.7%) in 120 women (26.3%) both microorganisms were present. the prevalence of vaginal mycoplasms is markedly influenced by the sexual activity of women. the relative risk of colonization of the vagina with m. hominis rises with the frequency of sexual intercourse more than four times. in case of a larger numbe ...19902196998
[evaluation of the mycoplasma plus and the sir mycoplasma kits for quantitative detection and antibiotic susceptibility testing of genital mycoplasma].we compared the results obtained with two commercially available systems (diagnostics pasteur) for the quantitative identification and the antibiotic susceptibility testing of the genital mycoplasmas. ureaplasma urealyticum and mycoplasma hominis with established methodologies, i.e. isolation on agar with enumeration by dilutions in broth medium and mic determinations. the mycoplasma plus system, consisting of six cups, was designed for the identification and quantitation of genital mycoplasmas ...19902195452
[role of mycoplasma hominis in acute infections of the female genital system].a study was carried out on the role of mycoplasma hominis in obstetric and gynecological infections, and the paper reports a case report of acute pelvoperitonitis in a woman fitted with an iud. the important role of early preoperative diagnosis in underlined and the current criteria for correct antibiotic therapy are discussed.19902187167
etiology and treatment of post-cesarean-section endometritis after cephalosporin prophylaxis.to describe the microbiologic etiology of post-cesarean endometritis developing after perioperative cephalosporin prophylaxis, endometrial samples were obtained from 27 women with a triple-lumen catheter. the women were assigned in a double-blind, randomized fashion to receive either ticarcillin/clavulanate, 3.1 g, or cefoxitin, 2 g, administered every six hours, until the clinical signs of infection resolved. a total of 149 microorganisms (84 facultative and 65 obligate anaerobes) were recovere ...19902181119
postgonococcal urethritis: a double-blind study of doxycycline vs placebo.the occurrence of postgonococcal urethritis (pgu) was studied in heterosexual men who were randomly assigned to receive either oral doxycycline 100 mg or a placebo capsule twice daily for seven days in addition to routine single dose treatment of urethral gonorrhoea. of 216 patients suitable for assessment, pgu was diagnosed in 54/104 (51.9%) in the placebo group and 30/112 (26.8%) in the doxycycline group. symptomatic pgu was uncommon (6.7% in the placebo group, 2.7% in the doxycycline group). ...19902179113
characterization of the tet m determinants in urogenital and respiratory bacteria.tetracycline-resistant fusobacterium nucleatum, haemophilus ducreyi, mycoplasma hominis, peptostreptococcus spp., ureaplasma urealyticum, and veillonella parvula had dna sequences which showed homology throughout the length of the tet m transposon, tn916. in contrast, gardnerella vaginalis, commensal neisseria spp., and the 25.2-megadalton plasmid family lacked the complete transposon.19902159259
pefloxacin compared with cefotaxime for treating men with uncomplicated gonococcal urethritis.in a randomized comparative study, 83 male patients suffering from acute uncomplicated gonococcal urethritis were treated with a single dose of either 0.8 g pefloxacin, given orally, or 1.0 g cefotaxime, given intramuscularly. the cure rates were 100% in both treatment groups four to seven days and 21 to 31 days, respectively, after therapy. the mics of the isolated neisseria gonorrhoeae ranged from 0.008 to 0.06 mg/l for pefloxacin and from 0.0005 to 0.03 mg/l for cefotaxime. postgonococcal ure ...19902124210
serologic evidence for the role of chlamydia trachomatis, neisseria gonorrhoeae, and mycoplasma hominis in the etiology of tubal factor infertility and ectopic pregnancy.the authors used enzyme immunoassay to determine the prevalence of serum antibodies to the sexually transmitted disease (std) organisms chlamydia trachomatis, neisseria gonorrhoeae, and mycoplasma hominis among 104 infertile women undergoing in vitro fertilization. altogether, 55 (72%) out of 76 women with tubal abnormalities tested positive for one or more std organisms, compared with only 6 (21%) out of 28 infertile women with normal tubes (p less than .001). the authors obtained positive test ...19902106171
mycoplasmas, a review of surveys examining human genital infections and experimental infection in mice with special reference to in vitro fertilization.patients attending an "in vitro fertilization" clinic were shown to be commonly infected in the semen with ureaplasma urealyticum (29%) and mycoplasma hominis (12%). other mycoplasmas were rarely recovered. m. hominis was not eradicated from sperm by washing, although washing removed 71% of the ureaplasmas from infected semen. there was a significantly higher incidence of u. urealyticum and m. hominis in patients with cervical intraepithelial neoplasia (p = less than 0.05 and p = less than 0.01 ...19902097465
increase in resistance of mycoplasma hominis to tetracyclines.isolates of mycoplasma hominis collected from patients in boston and new york between 1976 and 1989 were studied. minimal metabolism-inhibiting concentrations (mmcs) were determined by use of a terminal color change-broth dilution method, as well as by an agar inoculum-broth dilution method. both methods gave comparable results. tetracycline mmcs for 3 (7.0%) of 43 isolates of m. hominis collected from 1976 to 1979 were 8 micrograms/ml or more, but they were 8 micrograms/ml or more for 11 (20.3% ...19902088184
evaluation of the significance of mycoplasma hominis and ureaplasma urealyticum in female genital tract infection--a retrospective case note study.routine screening for sexually transmitted diseases in new patients attending the genitourinary clinic in stoke-on-trent includes a culture for mycoplasma hominis (mh) and ureaplasma urealyticum (uu). a retrospective study was carried out on 400 female patients to ascertain whether there were any significant differences between the group positive for mh and uu and the negative control group. the positive group were found to be younger on average, but to have similar sexual histories to the negat ...19902083293
mycoplasma hominis infection in three renal transplant patients.we describe three cases of extragenital infection by mycoplasma hominis in three patients transplanted with kidneys from cadaver donors. in two patients, the microorganism was isolated in the exudate from the surgical wound after 72 hrs. of culture on blood-agar (columbia + 5% horse blood) in co2 and under anaerobic conditions. in the remaining case, m. hominis was isolated in urine from a suprapubic catheter. all three patients responded satisfactorily to treatment with doxycycline. mycoplasma ...19902076594
[infections by mycoplasma hominis and ureaplasma urealytycum in vaginal discharge. incidence and treatment].this report summarizes the studies carried out on 100 patients who were examined for vaginal discharge. the following observations were made: mycoplasma hominis in 13.59% of the cases, ureaplasma urealytycum in 8.73% of the cases, that is, the presence of at least one of these microorganisms in 22.32 of the cases. all these infections were treated by tetracyclines administered in appropriate doses.19912068483
[value and limitations of the research of mycoplasma hominis and ureaplasma urealyticum in gastric fluid in newborn infants].mycoplasma hominis and ureaplasma urealyticum were cultured and counted in the gastric fluid of 153 neonates divided into three groups: 28 preterm neonates managed in an intensive care unit (group i); 83 full term neonates with suspected infection (group ii); and 42 full term neonates with not evidence of infection (group iii). the colonization rate (17.85%) in the intensive care unit group was not significantly different from the rates seem in the two other groups. these results do not militate ...19912062554
analysis of the nucleotide sequence of the mycoplasma hominis tuf gene and its flanking region.a gene from mycoplasma hominis pg21 similar to the tuf gene encoding the elongation factor tu (ef-tu) of escherichia coli was cloned and sequenced. the 1193-bp open reading frame flanked by a putative promoter and a potential stem-and-loop structure encoded a 44-kda polypeptide. the tuf gene of m. hominis pg21 has the lowest g + c content seen in prokaryotes (38.2%). a gene (mhlmp1) encoding a variable surface exposed membrane protein (lmp1) was found downstream the 3' end of the tuf gene. it wa ...19912060757
peritonitis due to mycoplasma hominis and ureaplasma urealyticum in a liver transplant recipient. 19912060519
isolation of mycoplasma hominis from peritoneal effluent of a capd patient. 19912049432
identification of a mycoplasmal protein which binds immunoglobulins nonimmunologically.immunoblotted protein samples from several strains of mycoplasma hominis and from one strain of mycoplasma arginini each contain a polypeptide of a molecular mass of 95,000 to 105,000 da which binds immunoglobulin nonimmunologically. immunoblots from these organisms were probed with alkaline phosphatase-conjugated goat anti-rabbit immunoglobulin, conjugated goat immunoglobulin g (igg) fab fragments, and conjugated goat igg fc fragments. the polypeptide bound the goat anti-rabbit molecules and th ...19912037376
mycoplasma hominis wound infection following aortobifemoral bypass. 19912037094
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