Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| molecular epidemiology of invasive listeriosis due to listeria monocytogenes in a spanish hospital over a nine-year study period, 2006-2014. | we investigated the pathogenicity, invasiveness, and genetic relatedness of 17 clinical listeria monocytogenes stains isolated over a period of nine years (2006-2014). all isolates were phenotypically characterised and growth patterns were determined. the antimicrobial susceptibility of l. monocytogenes isolates was determined in e-tests. invasion assays were performed with epithelial hela cells. finally, l. monocytogenes isolates were subtyped by pfge and mlst. all isolates had similar phenotyp ... | 2015 | 26539467 |
| multiplex pcr serogrouping of listeria monocytogenes isolated in japan. | pcr serogrouping methods were used to examine strains of l. monocytogenes isolated in japan. among 187 strains, 99.5% were classified into 4 pcr serogroups corresponding to conventional serotypes. only one isolate had a new pcr profile, which may be a variant of serogroup ivb. | 2016 | 26537550 |
| highly invasive listeria monocytogenes strains have growth and invasion advantages in strain competition. | multiple listeria monocytogenes strains can be present in the same food sample; moreover, infection with more than one l. monocytogenes strain can also occur. in this study we investigated the impact of strain competition on the growth and in vitro virulence potential of l. monocytogenes. we identified two strong competitor strains, whose growth was not (or only slightly) influenced by the presence of other strains and two weak competitor strains, which were outcompeted by other strains. cell co ... | 2015 | 26529510 |
| inactivation of the mapk signaling pathway by listeria monocytogenes infection promotes trophoblast giant cell death. | listeria monocytogenes has a well-characterized ability to cross the placental barrier, resulting in spontaneous abortion and fetal infections. however, the mechanisms resulting in infection-associated abortion are not fully understood. in this study, we demonstrate that the dephosphorylation of mapk family proteins caused by l. monocytogenes infection of trophoblast giant (tg) cells, which are placental immune cells, contributes to infectious abortion. dephosphorylation of c-jun, p38, and erk1/ ... | 2015 | 26528279 |
| phenotypes associated with the essential diadenylate cyclase cdaa and its potential regulator cdar in the human pathogen listeria monocytogenes. | cyclic diadenylate monophosphate (c-di-amp) is a second messenger utilized by diverse bacteria. in many species, including the gram-positive human pathogen listeria monocytogenes, c-di-amp is essential for growth. here we show that the single diadenylate cyclase of l. monocytogenes, cdaa, is an integral membrane protein that interacts with its potential regulatory protein, cdar, via the transmembrane protein domain. the presence of the cdar protein is not required for the membrane localization a ... | 2016 | 26527648 |
| comparative evaluation of veriflow® listeria monocytogenes to usda and aoac culture based methods for the detection of listeria monocytogenes in food. | veriflow® listeria monocytogenes (lm) is a molecular based assay for the presumptive detection of listeria monocytogenes from environmental surfaces, dairy, and ready-to-eat (rte) food matrixes (hot dogs and deli meat). the assay utilizes a pcr detection method coupled with a rapid, visual, flow-based assay that develops in 3 min post pcr amplification and requires only 24 h of enrichment for maximum sensitivity. the veriflow lm system eliminates the need for sample purification, gel electrophor ... | 2015 | 26525251 |
| method modification of the thermo scientific suretect listeria monocytogenes assay for raw meat, dairy, produce, and seafood. | the thermo scientific™ suretect™ listeria monocytogenes assay is a real-time pcr assay for the detection of listeria monocytogenes in food and environmental samples, which was certified during 2013 by the aoac research institute (ri) as performance tested method(sm) (ptm) 061302 for a representative range of key food matrixes and production surfaces. this report details the method modification study, which was conducted during 2014, using the aoac-ri ptm program to extend the validated matrix cl ... | 2015 | 26525250 |
| biofilm formation of the l. monocytogenes strain 15g01 is influenced by changes in environmental conditions. | listeria monocytogenes 15g01, a strain belonging to the persistent pulsotype 5132, was isolated from a seafood processing plant in new zealand. simple monoculture assays using crystal violet staining showed good biofilm formation for this strain and it was therefore chosen to be further investigated in regard to its biofilm forming ability. to evaluate its behaviour in different conditions commonly encountered in food processing environments, biofilm assays and growth studies were performed usin ... | 2015 | 26524221 |
| tracing sources of listeria contamination in traditional italian cheese associated with a us outbreak: investigations in italy. | in 2012 a us multistate outbreak of listeriosis was linked to ricotta salata imported from italy, made from pasteurized sheep's milk. sampling activities were conducted in italy to trace the source of listeria monocytogenes contamination. the cheese that caused the outbreak was produced in a plant in apulia that processed semi-finished cheeses supplied by five plants in sardinia. during an 'emergency sampling', 179 (23·6%) out of 758 end-products tested positive for l. monocytogenes, with concen ... | 2016 | 26522501 |
| effect of x-ray treatments on escherichia coli o157:h7, listeria monocytogenes, shigella flexneri, salmonella enterica and inherent microbiota on whole mangoes. | the aims of this investigation were to; (i) study the effect of x-ray treatments in reducing escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri on whole mangoes, and (ii) study the effect of x-ray treatments on microflora counts (mesophilic counts, psychrotrophic counts and yeast and mould counts) of whole mangoes during storage at ambient temperature (22°c) for 30 days. a mixture of three strains of each test organism was spot inoculated (100 μl; approx. ... | 2016 | 26518738 |
| listeria monocytogenes isolates from food and food environment harbouring tetm and ermb resistance genes. | listeria monocytogenes is a foodborne pathogen that has become an important cause of human and animal diseases worldwide. the purpose of this study was to evaluate the serotypes, virulence potential, antimicrobial resistance profile, and genetic relationships of 50 l. monocytogenes isolates from food and food environment in southern brazil. in this study, the majority of l. monocytogenes isolates belonged to the serotypes 1/2b (42%) and 4b (26%), which are the main serotypes associated with huma ... | 2016 | 26518475 |
| synergistic effect of copper and low temperature over listeria monocytogenes. | the capacity to grow at low temperatures has allowed listeria monocytogenes to become one of the primary food pathogens to date, representing a major public health problem worldwide. several works have described the homeostatic response of l. monocytogenes under different copper (cu) treatments growing at mild temperature (30 °c). the aims of this report were to evaluate if changes in the external concentration of cu affected viability and cu homeostasis of l. monocytogenes growing at low temper ... | 2015 | 26515293 |
| efflux pump-mediated benzalkonium chloride resistance in listeria monocytogenes isolated from retail food. | in this study, efflux pump-mediated benzalkonium chloride (bc) resistance, including plasmid-encoded (qac protein family and bcrabc) and chromosome-borne efflux pumps, was investigated in listeria monocytogenes from retail food in china. among the 59 l. monocytogenes strains, 13 (22.0%) strains were resistant to bc. the pcr results showed that bcrabc was harbored by 2 of 13 bc resistant strains. however, none of the qac genes were detected among the 59 strains. the bcrabc was absent in both of t ... | 2016 | 26513255 |
| characterization of a transferable bcrabc and cadac genes-harboring plasmid in listeria monocytogenes strain isolated from food products of animal origin. | in this study, we characterized a bcrabc cassette and its genetic environment harbored by a plasmid in listeria monocytogenes (l. monocytogenes) 11gzl18, a strain isolated from raw meat in 2011. the bcrabc cassette and its genetic environment were characterized, with a total of 33,727 nt nucleotide sequence obtained. the nucleotide sequences of the bcrabc cassette in strain 11gzl18 exhibited 100% identity to that on plasmid plm80, which is harbored by l. monocytogenes strain h7550 and h7858, and ... | 2016 | 26513251 |
| the intrinsic cephalosporin resistome of listeria monocytogenes in the context of stress response, gene regulation, pathogenesis and therapeutics. | intrinsic resistance to antibiotics is a serious therapeutic problem in the case of many bacterial species. the gram-positive human pathogen listeria monocytogenes is intrinsically resistant to broad spectrum cephalosporin antibiotics, which are commonly used in therapy of bacterial infections. besides three penicillin-binding proteins the intrinsic cephalosporin resistome of l. monocytogenes includes multidrug resistance transporter transporters, proteins involved in peptidoglycan biosynthesis ... | 2016 | 26509460 |
| lactobacillus plantarum lb95 impairs the virulence potential of gram-positive and gram-negative food-borne pathogens in ht-29 and vero cell cultures. | listeria monocytogenes, salmonella enterica and verocytotoxigenic escherichia coli (vtec) are amongst the most important agents responsible for food outbreaks occurring worldwide. in this work, two lactobacillus spp. strains (labs), lactobacillus plantarum (lb95) and lactobacillus paraplantarum (lb13), previously isolated from spontaneously fermenting olive brines, and two reference probiotic strains, lactobacillus casei shirota and lactobacillus rhamnosus gg, were investigated for their ability ... | 2016 | 26506821 |
| a tail of two phages: genomic and functional analysis of listeria monocytogenes phages vb_lmos_188 and vb_lmos_293 reveal the receptor-binding proteins involved in host specificity. | the physical characteristics of bacteriophages establish them as viable candidates for downstream development of pathogen detection assays and biocontrol measures. to utilize phages for such purposes, a detailed knowledge of their host interaction mechanisms is a prerequisite. there is currently a wealth of knowledge available concerning gram-negative phage-host interaction, but little by comparison for gram-positive phages and listeria phages in particular. in this research, the lytic spectrum ... | 2015 | 26500641 |
| cd8 t cells enter the splenic t cell zones independently of ccr7, but the subsequent expansion and trafficking patterns of effector t cells after infection are dysregulated in the absence of ccr7 migratory cues. | ccr7 is an important chemokine receptor that regulates t cell trafficking and compartmentalization within secondary lymphoid organs. however, the t cell-intrinsic role of ccr7 during infection in the spleen is not well understood. this study was designed to understand how ccr7-dependent localization and migration of cd8(+) t cells in different compartments of the spleen affected the primary and recall responses after infection. to this end, we used adoptive transfer of naive ag-specific cd8 t ce ... | 2015 | 26500349 |
| the connection between persistent, disinfectant-resistant listeria monocytogenes strains from two geographically separate iberian pork processing plants: evidence from comparative genome analysis. | the aim of this study was to investigate the basis of the putative persistence of listeria monocytogenes in a new industrial facility dedicated to the processing of ready-to-eat (rte) iberian pork products. quaternary ammonium compounds, which included benzalkonium chloride (bac), were repeatedly used as surface disinfectants in the processing plant. clean and disinfected surfaces were sampled to evaluate if resistance to disinfectants was associated with persistence. of the 14 isolates obtained ... | 2015 | 26497458 |
| a dual microscopy-based assay to assess listeria monocytogenes cellular entry and vacuolar escape. | listeria monocytogenes is a gram-positive bacterium and a facultative intracellular pathogen that invades mammalian cells, disrupts its internalization vacuole, and proliferates in the host cell cytoplasm. here, we describe a novel image-based microscopy assay that allows discrimination between cellular entry and vacuolar escape, enabling high-content screening to identify factors specifically involved in these two steps. we first generated l. monocytogenes and listeria innocua strains expressin ... | 2015 | 26497455 |
| enhanced antimicrobial effect of organic acid washing against foodborne pathogens on broccoli by vacuum impregnation. | this study was undertaken to evaluate the effect of vacuum impregnation applied to the washing process for removal of salmonella typhimurium and listeria monocytogenes from broccoli surfaces. broccoli was inoculated with the two foodborne pathogens and treated with simple dipping washing or with vacuum impregnation in 2% malic acid for 5, 10, 20, or 30 min. there were two methods of vacuum impregnation: continuous and intermittent. after 30 min of 101.3 kpa (=14.7 psi, simple dipping), 61.3 kpa ... | 2016 | 26496412 |
| clonal clustering using 10-gene multilocus sequence typing reveals an association between genotype and listeria monocytogenes maximum growth rate in defined medium. | we used a 10-gene (10g) multilocus sequence typing scheme to investigate the diversity and phylogenetic distribution of 124 listeria monocytogenes strains across major lineages, major serotypes, and seven epidemic clones that have been previously associated with outbreaks. the 124 isolates proved to be diverse, with a total of 81 sequence types (10g-sts) belonging to 13 clonal complexes (ccs), where all sts of the same cc differ from one another in up to 3 of the 10 alleles (named as 10g-triple- ... | 2015 | 26495863 |
| ring-enhancing brain lesions: listeria monocytogenes abscesses. | 2016 | 26494469 | |
| outbreak of hospital-acquired gastroenteritis and invasive infection caused by listeria monocytogenes, finland, 2012. | during one week in july 2012, two patients from the same ward at the municipal hospital in vaasa, finland, were diagnosed with septicaemia caused by listeria monocytogenes. an outbreak investigation revealed eight concomitant cases of febrile gastroenteritis caused by l. monocytogenes on the same ward. median age of the cases was 82 years and median incubation time for listerial gastroenteritis was 21 h (range 9-107). an additional 10 cases of invasive listeriosis caused by the same outbreak str ... | 2016 | 26493730 |
| antagonizing peroxisome proliferator-activated receptor α activity selectively enhances th1 immunity in male mice. | females exhibit more robust th1 responses than males. our previous work suggested that this sex disparity is a consequence of higher activity of the androgen-induced gene peroxisome proliferator-activated receptor α (pparα) in male cd4(+) t cells. the objective of this study was to elucidate the cellular and molecular mechanism of how pparα inhibits th1 responses in male mice. in this study, we found that pparα functions within cd4(+) and cd8(+) t lymphocytes and nkt cells to negatively regulate ... | 2015 | 26491197 |
| development of prototypes of bioactive packaging materials based on immobilized bacteriophages for control of growth of bacterial pathogens in foods. | due to lack of adequate control methods to prevent contamination in fresh produce and growing consumer demand for natural products, the use of bacteriophages has emerged as a promising approach to enhance safety of these foods. this study sought to control listeria monocytogenes in cantaloupes and rte meat and escherichia coli o104:h4 in alfalfa seeds and sprouts under different storage conditions by using specific lytic bacteriophage cocktails applied either free or immobilized. bacteriophage c ... | 2016 | 26490649 |
| inactivation of bacterial pathogens in yoba mutandabota, a dairy product fermented with the probiotic lactobacillus rhamnosus yoba. | mutandabota is a dairy product consumed as a major source of proteins and micronutrients in southern africa. in this study the microbial safety of traditional and a variant of mutandabota fermented with the probiotic lactobacillus rhamnosus yoba (yoba mutandabota) was investigated by challenging the products with five important food pathogens: listeria monocytogenes, salmonella spp., campylobacter jejuni, escherichia coli o157:h7 and bacillus cereus. pasteurized full-fat cow's milk was used for ... | 2016 | 26490648 |
| synergism between carvacrol or thymol increases the antimicrobial efficacy of soy sauce with no sensory impact. | here, we examined the antimicrobial effects of soy sauce containing essential oils (eos) against escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes at 22°c and 4°c. to screen a variety of combined effects, soy sauce was mixed with six different eos (carvacrol, thymol, eugenol, trans-cinnamaldehyde, β-resorcylic acid, and vanillin), each at a concentration of 1mm for 10 min. none of the oils showed bactericidal activity when used alone. soy sauce combined with carvacrol ... | 2016 | 26490647 |
| the listeria monocytogenes core-genome sequence typer (lmcgst): a bioinformatic pipeline for molecular characterization with next-generation sequence data. | next-generation sequencing provides a powerful means of molecular characterization. however, methods such as single-nucleotide polymorphism detection or whole-chromosome sequence analysis are computationally expensive, prone to errors, and are still less accessible than traditional typing methods. here, we present the listeria monocytogenes core-genome sequence typing method for molecular characterization. this method uses a high-confidence core (hcc) genome, calculated to ensure accurate identi ... | 2015 | 26490433 |
| systemic tolerance mediated by melanoma brain tumors is reversible by radiotherapy and vaccination. | immune responses to antigens originating in the central nervous system (cns) are generally attenuated, as collateral damage can have devastating consequences. the significance of this finding for the efficacy of tumor-targeted immunotherapies is largely unknown. | 2016 | 26490306 |
| novel active surface prepared by embedded functionalized clays in an acrylate coating. | the research on a self-decontaminating surface has received significant attention because of the growth of pathogenic microorganisms on surfaces. in this study, a novel and simple technique for producing an active surface with antimicrobial functionality is demonstrated. a tethering platform was developed by grafting the biocide ampicillin (amp) to a nanoclay and dispersing the nanoclay in a uv-curable acrylate coating applied on polypropylene films as the substrate. a coupling agent, [3-(glycid ... | 2015 | 26488557 |
| genome comparison of listeria monocytogenes serotype 4a strain hcc23 with selected lineage i and lineage ii l. monocytogenes strains and other listeria strains. | more than 98% of reported human listeriosis cases are caused by specific serotypes within genetic lineages i and ii. the genome sequence of listeria monocytogenes lineage iii strain hcc23 (serotype 4a) enables whole genomic comparisons across all three l. monocytogenes lineages. protein cluster analysis indicated that strain hcc23 has the most unique protein pairs with nonpathogenic species listeria innocua. orthology analysis of the genome sequences of representative strains from the three l. m ... | 2014 | 26484097 |
| prevalence, enumeration, and pheno- and genotypic characteristics of listeria monocytogenes isolated from raw foods in south china. | listeria monocytogenes is an important foodborne pathogen that can cause serious illness in immunocompromised individuals, pregnant women, the elderly, and newborns. the aim of this study was to: (i) evaluate the prevalence and contamination level [most probable number (mpn)] of l. monocytogenes in 567 retail raw foods (fishery products, n = 154; raw/fresh meat, n = 123; frozen foods, n = 110; edible fungi, n = 108; vegetables, n = 72) collected from south china and (ii) to gain further knowledg ... | 2015 | 26483765 |
| rna helicase important for listeria monocytogenes hemolytic activity and virulence factor expression. | rna helicases have been shown to be important for the function of rna molecules at several levels, although their putative involvement in microbial pathogenesis has remained elusive. we have previously shown that listeria monocytogenes dexd-box rna helicases are important for bacterial growth, motility, ribosomal maturation, and rrna processing. we assessed the importance of the rna helicase lmo0866 (here named csha) for expression of virulence traits. we observed a reduction in hemolytic activi ... | 2015 | 26483402 |
| pathogen-nematode interaction: nitrogen supply of listeria monocytogenes during growth in caenorhabditis elegans. | listeria monocytogenes is a gram-positive facultatively intracellular human pathogen. due to its saprophytic lifestyle, l. monocytogenes is assumed to infect and proliferate within soil organisms such as caenorhabditis elegans. however, little is known about the nutrient usages and metabolite fluxes in this bacterium-nematode interaction. here, we established a nematode colonization model for l. monocytogenes and a method for the efficient separation of the pathogen from the nematodal gut. follo ... | 2016 | 26478569 |
| the first mollusk spätzle homolog gene in the clam, paphia undulate. | spätzle, is the only identified endogenous toll receptor ligand, plays a critical role in initiatinge innate immune responses and controlling dorsal-ventral axis formation in drosophila. here we identified the first spätzle gene homolog, pu-spz, in the marine mollusk paphia undulate. the full-length of pu-spz cdna is 1248 bp, including an open reading frame (orf) of 702 bp, a 5'-untranslated region (utr) of 26 bp and a 3'-utr of 203 bp. the orf encodes a 233-amino-acid protein with conserved dom ... | 2015 | 26477575 |
| occurrence of salmonella, listeria monocytogenes, and enterotoxigenic staphylococcus in goat milk from small and medium-sized farms located in minas gerais state, brazil. | consumption of goat milk has been increasing due to its nutritional characteristics and health benefits. therefore, assessment of the presence of foodborne pathogens in this product is critical to ensure its safety to consumers. the present study aimed to identify common foodborne pathogens in raw goat milk. fifty-three samples of raw goat milk from 11 farms were collected and cultured for the presence of salmonella spp. and listeria monocytogenes, as well as for enumeration and isolation of coa ... | 2015 | 26476939 |
| virus recovering from strawberries: evaluation of a skimmed milk organic flocculation method for assessment of microbiological contamination. | skimmed milk organic flocculation method was adapted, optimized and compared with polyethylene glycol (peg) precipitation and filtration methods for recovering viruses from a strawberry matrix. spiking experiments with norovirus genogroup ii genotype 4 (nov gii.4) and murine norovirus 1 (mnv-1) demonstrated that the organic flocculation method associated with a glycine elution buffer, filter bag and cetyltrimethylammonium bromide (ctab) showed a recovery percentage of 2.5 and 32 times higher tha ... | 2016 | 26476572 |
| genetic diversity, virulence potential and antimicrobial susceptibility of listeria monocytogenes recovered from different sources in india. | listeria monocytogenes isolates (n = 36) recovered from human and animal clinical cases and foods from different geographical regions of india were characterized using multiplex pcr-based serotyping, pulsed field gel electrophoresis (pfge), in vitro and in vivo pathogenicity tests and antibiogram profiling. multiplex pcr-based serotyping distributed l. monocytogenes isolates into 3 serogroups, of which 91.67% belonged to 4b, 4d, 4e serogroup, followed by 5.56% to 1/2a, 3a and 2.78% to 1/2b, 3b s ... | 2015 | 26476275 |
| outbreak of listeria monocytogenes in pheasants. | listeria monocytogenes is capable of infecting almost all animals. however, outbreaks of listeriosis are infrequent in birds. this report describes an outbreak of listeriosis in a small pheasant (phasianus colchicus) breeder farm with more than 2,000 pheasants from hubei province of the people's republic of china. the affected flock consisted of adult and young birds. approximately 300 young birds and a few adult birds were found dead within a few days of the onset of clinical signs. twenty-five ... | 2015 | 26476090 |
| enteric bacteria isolated from diarrheal patients in korea in 2014. | the aim of this study was to characterize the pathogens responsible for causing diarrhea according to season, region of isolation, patient age, and sex as well as to provide useful data for the prevention of diarrheal disease. | 2015 | 26473090 |
| development of bioactive fish gelatin/chitosan nanoparticles composite films with antimicrobial properties. | the objective of this work was to develop active bio-based nanocomposite films from fish gelatin (fg) and chitosan nanoparticles (csnps) incorporated with origanum vulgare l. essential oil (oeo). csnps were obtained by ionic gelation of chitosan with sodium tripolyphosphate, which presented a spherical morphology with size range of 40-80nm. remarkable differences in the surface morphology were observed between the control and bioactive nanocomposite films as revealed by sem and afm images. ftir ... | 2016 | 26471681 |
| total phenolic and flavonoid content and antibacterial activity of punica granatum l. var. pleniflora flowers (golnar) against bacterial strains causing foodborne diseases. | flowers of punica granatum l. (punicaceae) var. pleniflora, known as "golnar" in iranian traditional medicine have been used for the prevention and treatment of foodborne diseases. in this study, antibacterial activities of ethanol extract of golnar and its fractions were scientifically evaluated against bacteria causing foodborne diseases including staphylococcus aureus, bacillus cereus, listeria monocytogenes, escherichia coli, shigella dysantriae, and salmonella typhi. the total phenolic and ... | 2015 | 26470879 |
| [biological activity of antimicrobial peptides of enterococcus faecium]. | isolate bacteriocins from enterococcus faecium metabolites and characterize their effect on cells of gram positive (listeria monocytogenes) and gram negative (escherichia coli) bacteria. | 2015 | 26470413 |
| macrophages mediate flagellin induced inflammasome activation and host defense in zebrafish. | the inflammasome is an innate immune complex whose rapid inflammatory outputs play a critical role in controlling infection; however, the host cells that mediate inflammasome responses in vivo are not well defined. using zebrafish larvae, we examined the cellular immune responses to inflammasome activation during infection. we compared the host responses with two listeria monocytogenes strains: wild type and lm-pyro, a strain engineered to activate the inflammasome via ectopic expression of flag ... | 2016 | 26468080 |
| antilisterial peptides released by enzymatic hydrolysis from grass carp proteins and activity on controlling l. monocytogenes inoculated in surimi noodle. | the primary objective of this study was to prepare the antilisterial peptides by enzymatic (pepsin, trypsin, protamex, neutrase, flavourzyme, papain, alcalase, and acid protease) hydrolysis of grass carp proteins with various degree of hydrolysis. the second objective was to evaluate the antilisterial activity of grass carp proteins hydrolysates (gcph) at differnet levels in the surimi noodle samples with or without boiling treatment inoculated with 10(4) cfu/g of listeria monocytogenes for stor ... | 2015 | 26467537 |
| comparative genomic analysis reveals that the 20k and 38k prophages in listeria monocytogenes serovar 4a strains lm850658 and m7 contribute to genetic diversity but not to virulence. | listeria monocytogenes is a foodborne pathogen of considerable genetic diversity with varying pathogenicity. initially, we found that the strain m7 was far less pathogenic than the strain lm850658 though both are serovar 4a strains belonging to the lineage iii. comparative genomic approaches were then attempted to decipher the genetic basis that might govern the strain-dependent pathotypes. there are 2,761 coding sequences of 100% nucleotide identity between the two strains, accounting for 95.7% ... | 2016 | 26464378 |
| growth of listeria monocytogenes within a caramel-coated apple microenvironment. | a 2014 multistate listeriosis outbreak was linked to consumption of caramel-coated apples, an unexpected and previously unreported vehicle for listeria monocytogenes. this outbreak was unanticipated because both the ph of apples (<4.0) and the water activity of the caramel coating (<0.80) are too low to support listeria growth. in this study, granny smith apples were inoculated with approximately 4 log10 cfu of l. monocytogenes (a cocktail of serotype 4b strains associated with the outbreak) on ... | 2015 | 26463161 |
| a small-scale experiment of using phage-based probiotic dietary supplement for prevention of e. coli traveler's diarrhea. | traveler's diarrhea (td) is caused by escherichia coli in 30% of cases. we have developed a phage cocktail for prophylaxis of td caused by e.coli, shigella flexneri, shigella sonnei, salmonella enterica, listeria monocytogenes or staphylococcus aureus, and investigated its effectiveness against infection caused by the non-pathogenic lac (-) strain of e.coli k12 c600 in animal and human trials. on the 6th day of both animal and human trials e. coli k12 c600 strain was detected in titer of 10(4) c ... | 2017 | 26458758 |
| identification and characterization of species-specific nanobodies for the detection of listeria monocytogenes in milk. | listeria monocytogenes (lm), one of the eight species belonging to the genus listeria, is pathogenic for both humans and animals. in this study, two novel lm-specific clones, designated l5-78 and l5-79, were isolated from a phage display antibody library that was derived from the variable domain of heavy-chain antibodies (vhhs) of non-immunized alpaca. these two clones were expressed, purified, and characterized. results showed that both isolated vhhs recognize three serotypes (1/2a, 1/2b, and 4 ... | 2016 | 26456330 |
| comparison of multiple‑locus variable number tandem repeat analysis and pulsed field gel electrophoresis in molecular subtyping of listeria monocytogenes isolates from italian cheese. | subtyping of listeria monocytogenes strains is an essential epidemiological tool to trace contamination and determine evolutionary relationships among different strains. pulsed field gel electrophoresis (pfge) is the current gold standard method for listeria characterization. multiple-locus variable-number tandem repeat analysis (mlva) is a rapid subtyping method based on polymerase chain reaction (pcr) amplification that has been successfully developed for subtyping bacterial genera. the purpos ... | 2015 | 26455371 |
| use of a miniature laboratory fresh cheese model for investigating antimicrobial activities. | hispanic-style fresh cheeses, such as queso fresco, have relatively low salt content, high water activity, and near neutral ph, which predisposes them to growth of listeria monocytogenes. biosafety constraints limit the incorporation of l. monocytogenes into cheeses manufactured via traditional methods in challenge studies, so few have focused on in situ testing of novel antimicrobials in fresh cheeses. we have developed a modular, miniaturized laboratory-scale queso fresco model for testing the ... | 2015 | 26454301 |
| in vitro generation of rabbit anti-listeria monocytogenes monoclonal antibody using single cell based rt-pcr linked cell-free expression systems. | rabbit monoclonal antibodies (mabs) have advantages over mouse antibodies in diagnostics and biotechnological applications owing to higher affinity and specificity.we developed a platform to generate rabbit mabs by a novel monoclonal antibody generation method named “single-cell reverse transcription-pcr linked in vitro-expression (sicrex)” system. in this method,we use single-cell based rt-pcr followed by sequential pcr steps of mab genes and subsequent cell-free protein synthesis (cfps) by usi ... | 2015 | 26454028 |
| survival and high-hydrostatic pressure inactivation of foodborne pathogens in salmorejo, a traditional ready-to-eat food. | salmorejo is a traditional tomato-based creamy product. because salmorejo is not heat-processed, there is a risk of contamination with foodborne pathogens from raw materials. even though bacterial growth in salmorejo is strongly inhibited because of its acidic ph (close to 3.9), the growth and survival of 3 foodborne pathogens in this food has not been studied before. in this study, 3 cocktails consisting of escherichia coli o157, salmonella enterica serovar enteritidis, and listeria monocytogen ... | 2015 | 26448479 |
| effect of honokiol on exotoxin proteins listeriolysin o and p60 secreted by listeria monocytogenes. | listeria monocytogenes is considered one of the most important foodborne pathogens. the virulence-related proteins listeriolysin o (llo) and p60 are critical factors involved in listeria pathogenesis. in the present study, we investigated the effect of honokiol on llo and p60 secreted from l. monocytogenes. a listeriolysin assay was used to investigate the haemolytic activities of l. monocytogenes exposed to honokiol, and the secretion of llo and p60 was detected by immunoblot analysis. addition ... | 2015 | 26445991 |
| development of listeriabase and comparative analysis of listeria monocytogenes. | listeria consists of both pathogenic and non-pathogenic species. reports of similarities between the genomic content between some pathogenic and non-pathogenic species necessitates the investigation of these species at the genomic level to understand the evolution of virulence-associated genes. with listeria genome data growing exponentially, comparative genomic analysis may give better insights into evolution, genetics and phylogeny of listeria spp., leading to better management of the diseases ... | 2015 | 26444974 |
| drosophila melanogaster natural variation affects growth dynamics of infecting listeria monocytogenes. | we find that in a listeria monocytogenes/drosophila melanogaster infection model, l. monocytogenes grows according to logistic kinetics, which means we can measure both a maximal growth rate and growth plateau for the microbe. genetic variation of the host affects both of the pathogen growth parameters, and they can vary independently. because growth rates and ceilings both correlate with host survival, both properties could drive evolution of the host. we find that growth rates and ceilings are ... | 2015 | 26438294 |
| a case of listeria monocytogenes endophthalmitis with recurrent inflammation and novel management. | listeria monocytogenes is a rare cause of endogenous endophthalmitis. in the limited number of reported listeria endophthalmitis cases, visual acuity outcomes have been very poor. | 2015 | 26437938 |
| [listeria monocytogenes meningitis in immunocompetent children: unpasteurized cheese likely cause of infection]. | listeria meningoencephalitis is a rare condition, occurring mainly in immunocompromised patients. we present two cases of listeria monocytogenes meningoencephalitis in immunocompetent children, with successful treatment with betalactam/aminoglycoside combination. unpasteurized cheese was postulated as the source of infection. | 2015 | 26436795 |
| characterization of listeria monocytogenes isolated from human listeriosis cases in china. | 2015 | 26421272 | |
| properties of lactobacillus reuteri chitosan-calcium-alginate encapsulation under simulated gastrointestinal conditions. | the protective effects of encapsulation on the survival of lactobacillus reuteri and the retention of the bacterium's probiotic properties under simulated gastrointestinal conditions were investigated. viable counts and the remaining probiotic properties of calcium (ca)-alginate encapsulated (a group), chitosan-ca-alginate encapsulated (ca group), and unencapsulated, free l. reuteri (f group) were determined. encapsulation improved the survival of l. reuteri subjected to simulated gastrointestin ... | 2015 | 26415668 |
| use of e-beam radiation to eliminate listeria monocytogenes from surface mould cheese. | camembert and brie soft cheese varieties were subjected to e-beam irradiation as a sanitation treatment. the effects of treatments on microbiota and selected physicochemical properties were also studied. the absorbed doses required to meet the food safety objective (fso) according to eu and usda criteria for listeria monocytogenes were 1.27 and 2.59 kgy, respectively. the bacterial load, mainly lactic acid bacteria, was reduced by the treatment but injured cells were recovered during storage at ... | 2015 | 26415665 |
| [the stimulating effect of exometabolites of the marine microalgae phaeodactylum tricornutum bohlin on reproduction of listeria monocytogenes]. | the biological activity of ethyl acetate extract of exometabolites from the marine microalgae phaeodactylum tricornutum against a test culture of listeria monocytogenes 4b has been investigated. it was shown that the stimulating effect of algal exometabolites on the growth of pathogenic bacteria increased maximally by 98.3% on day 6 of the cultivation, if the active substances comprising the culture liquid of p. tricornutum were separated by successive extractions with solvents in order of incre ... | 2016 | 26415278 |
| pathogen loading from canada geese faeces in freshwater: potential risks to human health through recreational water exposure. | canada geese (branta canadensis) faeces have been shown to contain pathogenic protozoa and bacteria in numerous studies over the past 15 years. further, increases in both the canada geese populations and their ideal habitat requirements in the united states (us) translate to a greater presence of these human pathogens in public areas, such as recreational freshwater beaches. combining these factors, the potential health risk posed by canada geese faeces at freshwater beaches presents an emerging ... | 2016 | 26414207 |
| optimization of growth and bacteriocin production by lactobacillus sakei subsp. sakei2a. | lactobacillus sakei subsp. sakei 2a is a bacteriocinogenic lactic acid bacterium isolated from brazilian pork sausage, capable of inhibiting the growth of microbial pathogens, mainly listeria monocytogenes. in order to optimize bacteriocin production for industrial applications, this study evaluated the effect of supplementation of mrs broth with glucose, tween 20, tween 80, sodium citrate, potassium chloride and cysteine, and effect of the initial ph and temperature of incubation of the medium ... | 2017 | 26413066 |
| astaxanthin from psychrotrophic sphingomonas faeni exhibits antagonism against food-spoilage bacteria at low temperatures. | food production and processing industry holds a perpetual relationship with microorganisms and their by-products. in the present study, we aimed to identify beneficial cold-adapted bacteria devoid of any food spoilage properties and study their antagonism against common food-borne pathogens at low temperature conditions. ten isolates were obtained on selective isolation at 5 °c, which were spread across genera pseudomonas, sphingomonas, psychrobacter, leuconostoc, rhodococcus, and arthrobacter. ... | 2015 | 26411893 |
| characterization of epidemic clones of listeria monocytogenes serotype 4b isolated from humans and meat products in brazil. | listeria monocytogenes is an important foodborne pathogen and the 4b serotype is responsible for many cases of human listeriosis reported in brazil. several listeriosis outbreaks worldwide have involved a small number of well-defined clonal groups, designated as epidemic clones (ecs). | 2015 | 26409737 |
| behavior of listeria monocytogenes in sliced ready-to-eat meat products packaged under vacuum or modified atmosphere conditions. | the objective of this research was to determine the behavior of listeria monocytogenes in three types of sliced ready-to-eat meat products packaged under vacuum or modified atmosphere conditions and stored at three temperatures. slices of about 25 g of chorizo (a fermented dry pork sausage), jamón (cured ham), and cecina (a salted, dried beef product) were inoculated with l. monocytogenes nctc 11994. slices were packaged in a vacuum or in a modified atmosphere (20% co2, 80% n2). after packaging, ... | 2015 | 26408140 |
| effects of two application methods of plantaricin bm-1 on control of listeria monocytogenes and background spoilage bacteria in sliced vacuum-packaged cooked ham stored at 4°c. | two application methods were used to investigate the effect of plantaricin bm-1 on the control of listeria monocytogenes and background spoilage bacteria in sliced vacuum-packaged cooked ham without the addition of any chemical preservatives, including sodium nitrite, during 35 days of storage at 4°c. regardless of the application method, plantaricin bm-1 treatment (320, 640, or 1,280 arbitrary units [au]/g of sliced cooked ham) significantly (p < 0.05) reduced the survival of l. monocytogenes ( ... | 2015 | 26408132 |
| persistence of indicator and pathogenic microorganisms in broccoli following manure spreading and irrigation with fecally contaminated water: field experiment. | in 2011 and 2012, trials consisting of experimental plots were carried out to evaluate the presence of pathogenic (listeria monocytogenes, salmonella) and prevalence of indicator (escherichia coli) microorganisms in broccoli fertilized with liquid hog manure or mineral fertilizers and irrigated zero, one, or two times with e. coli-contaminated water. in 2011, results showed that e. coli contamination in broccoli heads was affected by the interval between irrigation and sampling (p = 0.0236), wit ... | 2015 | 26408125 |
| prevalence and distribution of listeria monocytogenes inla alleles prone to phase variation and inla alleles with premature stop codon mutations among human, food, animal, and environmental isolates. | in listeria monocytogenes, 18 mutations leading to premature stop codons (pmscs) in the virulence gene inla have been identified to date. while most of these mutations represent nucleotide substitutions, a frameshift deletion in a 5' seven-adenine homopolymeric tract (ht) in inla has also been reported. this ht may play a role in phase variation and was first identified among l. monocytogenes lineage ii ribotype dup-1039c isolates. in order to better understand the distribution of different inla ... | 2015 | 26407886 |
| isolation and characterization of bacteriocinogenic lactic bacteria from m-tuba and tepache, two traditional fermented beverages in méxico. | mexican tuba (m-tuba) and tepache are mexican fermented beverages prepared mainly with pineapple pulp and coconut palm, respectively. at present, reports on the microbiota and nutritional effects of both beverages are lacking. the purpose of this study was to determine whether m-tuba and tepache contain cultivable lactic acid bacteria (lab) capable of producing bacteriocins. tepache and m-tuba contain mesophilic aerobic bacteria, lab, and yeast. bacillus subtilis, listeria monocytogenes, listeri ... | 2015 | 26405529 |
| the noa gene is functionally linked to the activation of the toll/imd signaling pathways in bactrocera dorsalis (hendel). | the noa gene is an essential gene encoding a very long chain fatty acid elongase. in this study, we cloned the noa gene of bactrocera dorsalis, which encodes a protein sharing 84.50% identity to the noa in drosophila melanogaster. the expression profiles indicated that the transcriptional level of noa was high at the egg stage and in the testis tissue. the results showed that noa expression was up-regulated after listeria monocytogenes, staphylococcus aureus and escherichia coli infection. silen ... | 2016 | 26404497 |
| phytochemical characterization of veronica officinalis l., v. teucrium l. and v. orchidea crantz from romania and their antioxidant and antimicrobial properties. | aerial parts of veronica species are used in romanian traditional medicine for the treatment of various conditions like kidney diseases, cough, and catarrh, and are known for their wound-healing properties. in the present study, the phenolic and sterolic content and the antioxidant and antimicrobial activities of three veronica species (plantaginaceae), v. officinalis l., v. teucrium l. and v. orchidea crantz, were studied. the identification and quantification of several phenolic compounds and ... | 2015 | 26404257 |
| secondary metabolites constituents and antioxidant, anticancer and antibacterial activities of etlingera elatior (jack) r.m.sm grown in different locations of malaysia. | etlingera elatior is a well-known herb in malaysia with various pharmaceutical properties. | 2015 | 26399961 |
| antibacterial effect and mechanism of high-intensity 405 ± 5 nm light emitting diode on bacillus cereus, listeria monocytogenes, and staphylococcus aureus under refrigerated condition. | this study investigated the antibacterial effect of 405 ± 5 nm light emitting diode (led) on bacillus cereus, listeria monocytogenes, and staphylococcus aureus, and examined its antibacterial mechanism by determining the bacterial membrane and dna damages. a 405 ± 5 nm led illuminated the gram-positive pathogens until 486 j/cm(2) at 4 °c. weibull model was used to calculate reliable life (tr) to compare bacterial sensitivities to led illumination. the membrane damage was determined by nacl and l ... | 2015 | 26398810 |
| in situ production of pediocin pa-1 like bacteriocin by different genera of lactic acid bacteria in soymilk fermentation and evaluation of sensory properties of the fermented soy curd. | the lactic acid bacteria (lab) are found to produce bacteriocins with enhanced nutritive properties in the fermented foods. in the present study, the ability of lab cultures (pediococcus acidilactici ncim 5424, enterococcus faecium ncim 5423 and lactobacillus plantarum acr2) to produce pediocin pa-1 like bacteriocin was evaluated during soymilk fermentation. the isolates e. faecium ncim 5423 and lb. plantarum acr2 were able to produce bacteriocin as well as ferment soymilk within 6 h of incubati ... | 2014 | 26396327 |
| prevalence and virulence characterization of listeria monocytogenes in chilled pork in zhejiang province, china. | listeria monocytogenes is an important foodborne pathogen that can grow in refrigeration temperature and causes severe human infections. the aims of this work were to estimate the prevalence of l. monocytogenes in chilled pork in zhejiang, china and to examine the virulence features of the isolates. of 331 meat samples, 196 were positive for listeria spp., with l. innocua accounting for 54.4%, l. monocytogenes for 11.5%, and l. welshimeri for 4.2%. the most prevalent l. monocytogenes serotype wa ... | 2016 | 26393675 |
| a physical/psychological and biological stress combine to enhance endoplasmic reticulum stress. | the generation of an immune response against infectious and other foreign agents is substantially modified by allostatic load, which is increased with chemical, physical and/or psychological stressors. the physical/psychological stress from cold-restraint (cr) inhibits host defense against listeria monocytogenes (lm), due to early effects of the catecholamine norepinephrine (ne) from sympathetic nerves on β1-adrenoceptors (β1ar) of immune cells. although cr activates innate immunity within 2h, h ... | 2015 | 26391182 |
| using uvc light-emitting diodes at wavelengths of 266 to 279 nanometers to inactivate foodborne pathogens and pasteurize sliced cheese. | uvc light is a widely used sterilization technology. however, uv lamps have several limitations, including low activity at refrigeration temperatures, a long warm-up time, and risk of mercury exposure. uv-type lamps only emit light at 254 nm, so as an alternative, uv light-emitting diodes (uv-leds) which can produce the desired wavelengths have been developed. in this study, we validated the inactivation efficacy of uv-leds by wavelength and compared the results to those of conventional uv lamps ... | 2015 | 26386061 |
| propofol increases host susceptibility to microbial infection by reducing subpopulations of mature immune effector cells at sites of infection. | anesthetics are known to modulate host immune responses, but separating the variables of surgery from anesthesia when analyzing hospital acquired infections is often difficult. here, the bacterial pathogen listeria monocytogenes (lm) was used to assess the impact of the common anesthetic propofol on host susceptibility to infection. brief sedation of mice with physiologically relevant concentrations of propofol increased bacterial burdens in target organs by more than 10,000-fold relative to inf ... | 2015 | 26381144 |
| antibacterial activities of ankaferd hemostat (abs) on shiga toxin-producing escherichia coli and other pathogens significant in foodborne diseases. | ankaferd hemostat (ankaferd blood stopper®, abs)-induced pharmacological modulation of essential erythroid proteins can cause vital erythroid aggregation via acting on fibrinogen gamma. topical endoscopic abs application is effective in the controlling of gastrointestinal (gi) system hemorrhages and/or infected gi wounds. escherichia coli o157:h7, the predominant serotype of enterohemorrhagic e. coli, is a cause of both outbreaks and sporadic cases of hemorrhagic colitis. the aim of this study i ... | 2017 | 26377624 |
| combined effect of ultrasound and essential oils to reduce listeria monocytogenes on fresh produce. | salads prepared from contaminated fresh produce have a high risk of causing food-borne illnesses. essential oils obtained from plants have antimicrobial activity and may provide a natural approach to reduce the pathogens on fresh produce. additionally, ultrasound treatments have been shown to reduce the microbial counts on different foods. the objective of this study was to investigate the antimicrobial activities of cinnamon and lemon essential oils in vitro and in food applications. mixtures o ... | 2016 | 26377335 |
| nosocomial neonatal listeria monocytogenes transmission by stethoscope. | 2015 | 26376314 | |
| an unusual presentation of paediatric listeria meningitis with selective spinal grey matter involvement and acute demyelinating polyneuropathy. | paediatric listeria meningitis is rare, especially in immuno-competent children, but associated with significant mortality and morbidity and frequent complications. | 2016 | 26371981 |
| potential bio-control agent from rhodomyrtus tomentosa against listeria monocytogenes. | listeria monocytogenes is an important foodborne pathogen implicated in many outbreaks of listeriosis. this study aimed at screening for the potential use of rhodomyrtus tomentosa ethanolic leaf extract as a bio-control agent against l. monocytogenes. twenty-two l. monocytogenes isolates were checked with 16 commercial antibiotics and isolates displayed resistance to 10 antibiotics. all the tested isolates were sensitive to the extract with inhibition zones ranging from 14 to 16 mm. minimum inhi ... | 2015 | 26371033 |
| domain function dissection and catalytic properties of listeria monocytogenes p60 protein with bacteriolytic activity. | the major extracellular protein p60 of listeria monocytogenes (lm-p60) is an autolysin that can hydrolyze the peptidoglycan of bacterial cell wall and has been shown to be required for l. monocytogenes virulence. the predicted three-dimensional structure of lm-p60 showed that lm-p60 could be split into two independent structural domains at the amino acid residue 270. conserved motif analysis showed that v30, d207, s395, and h444 are the key amino acid residues of the corresponding motifs. howeve ... | 2015 | 26363556 |
| cutting edge: the orphan nuclear receptor nr4a1 regulates cd8+ t cell expansion and effector function through direct repression of irf4. | the transcription factor ifn regulatory factor (irf)4 was shown to play a crucial role in the protective cd8(+) t cell response; however, regulation of irf4 expression in cd8(+) t cells remains unclear. in this article, we report a critical role for nr4a1 in regulating the expansion, differentiation, and function of cd8(+) t cells through direct transcriptional repression of irf4. without nr4a1, the regulation of irf4 is lost, driving an increase in irf4 expression and, in turn, resulting in a f ... | 2015 | 26363057 |
| meta-analysis of the effects of sanitizing treatments on salmonella, escherichia coli o157:h7, and listeria monocytogenes inactivation in fresh produce. | the aim of this study was to perform a meta-analysis of the effects of sanitizing treatments of fresh produce on salmonella spp., escherichia coli o157:h7, and listeria monocytogenes. from 55 primary studies found to report on such effects, 40 were selected based on specific criteria, leading to more than 1,000 data on mean log reductions of these three bacterial pathogens impairing the safety of fresh produce. data were partitioned to build three meta-analytical models that could allow the asse ... | 2015 | 26362982 |
| biofilm-forming abilities of listeria monocytogenes serotypes isolated from different sources. | a total of 98 previously characterized and serotyped l. monocytogenes strains, comprising 32 of 1/2a; 20 of 1/2b and 46 of 4b serotype, from clinical and food sources were studied for their capability to form a biofilm. the microtiter plate assay revealed 62 (63.26%) strains as weak, 27 (27.55%) strains as moderate, and 9 (9.18%) strains as strong biofilm formers. among the strong biofilm formers, 6 strains were of serotype 1/2a and 3 strains were of serotype 1/2b. none of the strain from 4b ser ... | 2015 | 26360831 |
| listeria meningitis complicating a patient with ulcerative colitis on concomitant infliximab and hydrocortisone. | infliximab, a monoclonal antibody directed against tumour necrosis factor, is an effective therapy for moderate-to-severe ulcerative colitis and crohn's disease. uncommonly, serious opportunistic infections have occurred in patients after infliximab administration. here, we describe meningitis caused by listeria monocytogenes developing in a 37-year-old man with ulcerative colitis refractory to intravenous corticosteroids 10 days after receiving his first infusion of infliximab. with the increas ... | 2016 | 26358724 |
| mining of novel species-specific primers for pcr detection of listeria monocytogenes based on genomic approach. | listeria monocytogenes in contaminated food is considered as a serious health threat for consumers due to its high mortality rate. the objective of this study was to obtain novel species-specific target-genes and primers for the molecular detection of l. monocytogenes using a comparative genomic approach. by comparative analysis of l. monocytogenes and non-l. monocytogenes genome sequences in the genbank database with blast program, 26 specific target sequences were used as candidates and the pr ... | 2015 | 26354019 |
| combination of zinc and all-trans retinoic acid promotes protection against listeria monocytogenes infection. | zinc (zn) is the second most abundant transition metal after iron. it plays a vital role in living organisms and affects multiple aspects of the immune system. all-trans retinoic acid (atra) is an isomeric form of the vitamin a or retinol. it possesses the greatest biological activity of vitamin a. vitamin a and related retinoids influence many aspects of immunity. in this study, we demonstrated that treatment with a combination of zn and atra contributes to host resistance against infection by ... | 2015 | 26351852 |
| chemical composition and in vitro antibacterial activity of mentha spicata essential oil against common food-borne pathogenic bacteria. | the aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of mentha spicata plant against common food-borne pathogenic bacteria (staphylococcus aureus, bacillus subtilis, bacillus cereus, listeria monocytogenes, salmonella typhimurium, and escherichia coli o157:h7). chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (gc-ms). the antibacterial activity of the es ... | 2015 | 26351584 |
| reduction of listeria monocytogenes and bacillus cereus in milk by zinc oxide nanoparticles. | direct addition of antimicrobial materials to food during food processing is an effective method for controlling microbial contaminants of food and extending the shelf- life of food products. objective of this research was to study the antimicrobial effect of zinc oxide (zno) nanoparticle and potential applications of zno nanoparticles in terms of controling two food-borne pathogens in milk. | 2015 | 26351469 |
| intra- and inter-species interactions within biofilms of important foodborne bacterial pathogens. | a community-based sessile life style is the normal mode of growth and survival for many bacterial species. under such conditions, cell-to-cell interactions are inevitable and ultimately lead to the establishment of dense, complex and highly structured biofilm populations encapsulated in a self-produced extracellular matrix and capable of coordinated and collective behavior. remarkably, in food processing environments, a variety of different bacteria may attach to surfaces, survive, grow, and for ... | 2015 | 26347727 |
| fisetin protects against listeria monocytogenes infection by reducing the production of listeriolysin o. | 2016 | 26347572 | |
| polymer-based microfluidic chip for rapid and efficient immunomagnetic capture and release of listeria monocytogenes. | infections caused by foodborne pathogens such as listeria monocytogenes pose a threat to public health while timely detection is challenging due to pathogen low numbers. the development of robust and efficient sample preparation techniques is crucial to improve detection sensitivity and workflow. immunomagnetic separation using magnetic nanoparticles (mnps) is attractive, as it can efficiently capture target cells. for food safety applications, a platform is needed to rapidly process large sampl ... | 2015 | 26346021 |
| a study on characterization of new bacteriocin produced from a novel strain of lactobacillus spicheri g2 isolated from gundruk- a fermented vegetable product of north east india: a novel bacteriocin production from lactobacillus spicheri g2. | bacteriocin producing lactobacillus spicheri g2, isolated from gundruk - a traditional fermented vegetable product of north east india. l. spicheri g2 identified by morphological, biochemical techniques followed by 16s rrna gene technique. the 16sr rna sequence of bacteriocin producer is registered in ncbi under accession no. jx481912. the bacteriocin producing potential of l. spicheri is being reported for the first time in the present investigation. bacteriocin of l. spicheri g2 showed strong ... | 2015 | 26344995 |
| nonclinical development of bcg replacement vaccine candidates. | the failure of current mycobacterium bovis bacille calmette-guérin (bcg) vaccines, given to neonates to protect against adult tuberculosis and the risk of using these live vaccines in hiv-infected infants, has emphasized the need for generating new, more efficacious and safer replacement vaccines. with the availability of genetic techniques for constructing recombinant bcg (rbcg) strains containing well-defined gene deletions or insertions, new vaccine candidates are under evaluation at both the ... | 2013 | 26343962 |