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circulating antibodies after experimental chronic infection in the root canal of teeth in monkeys.root canals of 49 teeth with necrotic pulp tissue in five monkeys were infected with streptococcus faecalis ss liquefaciens, actinomyces bovis, fusobacterium nucleatum, peptostreptococcus anaerobius, and bacteroides oralis in various combinations. after 6 months the root canals and the periapical tissues were subjected to radiographic, bacteriologic, and histologic examinations. signs of periapical inflammation were radiographically registered in 41 teeth, most frequently in teeth inoculated wit ...19826960463
characterization of nadp-dependent 7 beta-hydroxysteroid dehydrogenases from peptostreptococcus productus and eubacterium aerofaciens.peptostreptococcus productus strain b-52 (a human fecal isolate) and eubacterium aerofaciens atcc 25986 were found to contain nadp-dependent 7 beta-hydroxysteriod dehydrogenase activity. the enzyme was synthesized constitutively by both organisms, and the enzyme yields were suppressed by the addition of 0.5 mm 7 beta-hydroxy bile acid to the growth medium. purification of the enzyme by chromatography resulted in preparations with 3.5 (p. productus b-52, on sephadex g-200) and 1.8 (e. aerofaciens ...19826954878
n-formimidoyl thienamycin (mk0787): in vitro activity against anaerobic bacteria.the in vitro activity of n-formimidoyl thienamycin (mk0787) was tested against 239 anaerobic bacteria clinical isolates: 70 of bacteroides fragilis, 18 of b. distasonis, 16 of b. thetaiotaomicron, 10 of b. vulgatus, 24 of bacteroides spp., 22 of b. melaninogenicus (all three subspecies), 26 of fusobacterium spp., 10 of peptococcus spp., 15 of peptostreptococcus spp., 15 of clostridium perfringens, and 13 of clostridium spp. ninety-five percent of the isolates were inhibited by less than or equal ...19826952818
[study on anaerobic gram-positive cocci in human dental plaque (author's transl)]. 19816950002
[comparative study of the flora of the pocket and gingival sulcus]. 19816949889
bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy.the presence of bacteria in 17 single-rooted teeth, with periapical lesions, was studied throughout a whole period of treatment. the root canals were irrigated with physiologic saline solution during instrumentation. no antibacterial solutions or dressings were used. bacteria were found in all initial specimens from the teeth (median number of bacterial cells 4 x 10(5), range 10(2) - 10(7)) and the number of strains in the specimens ranged from 1 to 10.88% of the strains were anaerobic. the most ...19816947391
n-formimidoyl-thienamycin activity against anaerobes: effect of the inoculum, ph and culture media. 19816945303
[a bacteriological study of peritonsillar abscess and its chemotherapy (author's transl)]. 19806942141
heparin degradation by eubacterium and peptostreptococcus species from bovine endometritis. 19826890997
anaerobic bacteria in uterine infection following first trimester abortion.samples of uterine fluid and cervical mucus for bacterial culture were obtained from 23 patients with endometritis following first trimester abortion. uterine samples were obtained via a sheathed system which minimizes contamination by the cervical flora. a mean of 3.4 isolates were identified in cervical samples as compared to 1.8 in uterine samples. anaerobic bacteria were found in 57% of the uterine samples and dominated in both uterine and cervical samples. peptostreptococcus species dominat ...19826890898
[serodiagnosis of crohn's disease].three serological tests, recommended as being of diagnostic value for crohn's disease, were evaluated in 39 patients with crohn's disease and--as controls--in 27 patients with ulcerative colitis, 45 healthy persons and 65 patients with inflammatory diseases other than crohn's disease or ulcerative colitis. the tests were the determination of (1) serum antibodies to pseudomonas-like organisms (plo) by means of indirect immunofluorescence; (2) agglutinating serum antibodies to 4 strains of anaerob ...19836872878
ct in the diagnosis and follow-up of multiple abscesses in the liver.computed tomography (ct) in the diagnosis of liver abscesses is discussed. the accuracy of ct scan in differentiating hepatic masses is reviewed. two ct control scans were performed during the follow-up period.19836872821
[nonclostridial anaerobic soft tissue infections].fourteen cases of nonclostridial anaerobic infections of soft tissues caused by peptococci, peptostreptococci, bacteroids or their combinations with e. coli, proteus, and staphylococcus aureus were studied. they are characterized by a rapid spread of serous-suppurative inflammation and the causative agents in loose fibrous connective tissue of subcutaneous adipose tissue (cellutitis), surface fascia (fasciitis), or skeletal muscles (myositis). dissolution of intercellular structures of the conne ...19836860161
aspiration and lung abscess in cystic fibrosis.large abscess cavities are not commonly encountered in patients with cystic fibrosis (cf). one such patient is reported in whom an abscess developed secondary to aspiration of sand and salt water. material obtained at bronchoscopy revealed anaerobes as well as the aerobic organisms previously cultured from her sputum. rapid improvement occurred after institution of antibiotic therapy specifically directed against the anaerobic organisms.19836859663
effect of eight growth media upon fermentation profiles of ten anaerobic bacteria.a study was made of the influence of eight growth media upon the fermentation end-product patterns obtained with ten species of anaerobes. acidic and neutral end-products of fermentation were analysed by gas chromatography and the results were examined statistically by computer. differences in end-product profile were at least as great between different media used to grow a single anaerobic species, as between different anaerobes grown in a single type of medium. when individual fermentation end ...19836853401
anaerobic bacteria associated with osteomyelitis in domestic animals.specimens of bony tissue or adjacent soft tissue from 19 animals with osteomyelitis were cultured aerobically and anaerobically. fourteen specimens (74%) yielded anaerobic bacteria in pure culture or mixed with aerobic or facultative anaerobic bacteria. the most predominant genus encountered was an obligate anaerobe, bacteroides. the most frequently isolated bacteroides species was bacteroides asaccharolyticus, which was isolated 5 times. the most frequently isolated anaerobe was peptostreptococ ...19836853311
perineal crohn's disease complicated by pyogenic liver abscess during metronidazole therapy.a patient with ileal and perineal crohn's disease developed a pyogenic liver abscess caused by streptococcus milleri. the perineal process had been treated with high-dose metronidazole for the preceding 6 mo, and culture of a perianal abscess 2 mo before admission revealed heavy growth of streptococcus milleri (which was misidentified as an anaerobic peptostreptococcus). this case illustrates the difficulty in correctly identifying streptococcus milleri, the most frequent isolate from pyogenic l ...19836852451
anaerobic pyogenic sacroiliitis. 19836844970
effect of glucose concentration in the growth medium upon neutral and acidic fermentation end-products of clostridium bifermentans, clostridium sporogenes and peptostreptococcus anaerobius.clostridium bifermentans, c. sporogenes and peptostreptococcus anaerobius were grown in fastidious anaerobe broth with glucose concentrations from 0-1.5% (w/v). gas chromatographic (glc) analysis of fermentation end-products revealed that a change in glucose concentration markedly affected fermentation patterns of each microorganism. increased glucose concentration resulted in stimulation of ethanol production by all strains, and of butanol and butyric acid by c. sporogenes. with c. bifermentans ...19836822993
pediatric infectious cervical lymphadenitis.infectious cervical lymphadenitis is a frequently encountered problem in the pediatric population. the recent experience with infectious cervical lymphadenitis at the children's hospital of philadelphia was reviewed. the most common etiologic agent was staphylococcus aureus. beta-hemolytic streptococcus and mycobacterium were found in 15% and 6% of cases respectively. other agents included pneumococcus, cat scratch, toxoplasma gondii, actinomycosis, and mixed bacteroides and peptostreptococcus. ...19806821410
leucine dissimilation to isovaleric and isocaproic acids by cell suspensions of amino acid fermenting anaerobes: the stickland reaction revisited.freshly compared cell suspensions of clostridia (clostridium bifermentans, c. botulinum proteolytic type a, c. difficile, c. sordellii, and c. sporogenes) and peptostreptococcus anaerobius converted leucine to isovaleric (iv) and isocaproic (ic) acids in the absence of other amino acids. the optimal ph for conversion was between 8 and 9 at 37 degrees c. the stoichiometry of reaction was compatible with that expected for the stickland reaction, as the ratio of iv to ic was 1:2, the amount of co2 ...19826805929
the fermentation of lactulose by colonic bacteria.sixty-four strains of intestinal bacteria were cultured under anaerobic conditions in lactulose-containing media to assess their ability to ferment lactulose. some organisms were unable to metabolize the disaccharide, while others, e.g. clostridia and lactobacilli, metabolized lactulose extensively. quantitative analyses of the fermentation products indicated that the major non-gaseous metabolites were acetic, lactic and butyric acids. hydrogen and carbon dioxide were the only gases detected. fe ...19826804597
bacterial interference between clostridium difficile and normal fecal flora.clostridium difficile has been shown to be the cause of virtually all cases of pseudomembranous colitis related to the administration of antimicrobial agents. it is possible that some antimicrobial agents alter the normal bacterial flora of the gastrointestinal tract so as to permit colonization and/or proliferation by c. difficile. the inhibitory activity of representative fecal bacteria from 23 anaerobic and aerobic genera against c. difficile was examined using two in vitro procedures. strain ...19816785366
serum antibodies to anaerobic coccoid rods in patients with crohn's disease or ulcerative colitis, and in medical and nursing staff. 19816779893
kinetic parameters of lactate dehydrogenases of some rumen bacterial species, the anaerobic ciliate isotricha prostoma and mixed rumen microorganisms.a number of kinetic parameters of the lactate dehydrogenases of three rumen bacterial species (peptostreptococcus productus, propionibacterium acnes and actinomyces viscosus), the rumen ciliate isotricha prostoma and mixed rumen microorganisms (mrm) with respect to nadh, pyruvate, fructose-1,6-diphosphate (fdp) as well as the effects of several nucleotide phosphates were studied. partially purified ldh of peptostr. productus had the same kinetic parameters as in crude cell free extracts. values ...19806778389
antigenic relationships and rapid identification of peptostreptococcus species.antisera against whole cells of each peptostreptococcus species (p. anaerobius, p. micros, p. parvulus, and p. productus) were produced in rabbits. when these antisera were reacted against sonically disrupted cells and culture supernatant fluids in ouchterlony tests, lines of identity were obtained among the antigens from all the species and uninoculated culture medium. when the antisera were subsequently absorbed with the dehydrated culture medium used to grow the peptostreptococci, all cross-r ...19806769956
antibiotic resistance in anaerobic bacteria: molecular biology and clinical aspects.the patterns of antibiotic susceptibility among anaerobes isolated in the united states during the past 14 years were reviewed. resistance to the tetracyclines, the penicillins, clindamycin, and other antibiotics has emerged among strains of bacteroides, clostridium, and anaerobic cocci. genetic transfer of antibiotic resistance has been documented in anaerobic environments. inter- and intrageneric transfer by conjugation and transformation has been described. plasmids have been identified in an ...19826760336
achromopeptidase for lysis of anaerobic gram-positive cocci.achromopeptidase, which has potent bacteriolytic activity for most of the gram-positive aerobic bacteria, was for the first time used for the lysis of anaerobic cocci. most of the lysozyme-resistant gram-positive anaerobic cocci were lysed with this new enzyme. peptococcus magnus was the only organism tested resistant to achromopeptidase. p. saccharolyticus was quite unusual because it was very sensitive to both achromopeptidase and lysozyme.19826759529
bacteriologic and serologic investigation in diffuse sclerosing osteomyelitis (dso) of the mandible.an attempt was made to clarify the obscure origin of diffuse sclerosing osteomyelitis (dso) of the mandible. bacteriologic investigations of specimens from diseased mandibles were performed with special attention to anaerobic culture technique. propionibacterium acnes and peptostreptococcus intermedius were in some patients found to be of etiologic importance. sampling techniques and associated problems are discussed. an examination was made for antibodies in the patients' sera against antigens ...19826757825
isolation of anaerobic organisms from kidney in serious renal infections.we have studied 45 patients who underwent nephrectomy owing to unilateral renal sepsis for anaerobic and aerobic bacterial growth in the urine and kidney. anaerobic organisms were recovered from 11 patients: 10 had positive kidney cultures, and only 1 had positive kidney and urine cultures. there was a distinct relationship between anaerobic infection of the kidney and urinary tract obstruction; 44 per cent of the obstructed kidneys yielded anaerobic organisms versus only 11 per cent of the unob ...19826755857
[evaluation of clinical efficacy of a cephem antibiotic, cefmetazole, in inflammatory infections of the upper respiratory tract].we had reported the fundamental study on the utility of a new cephem antibiotic, cefmetazole (cmz). on the basis of the results we administered cmz to patients to investigate its clinical utility in this study. 1. cmz was administered to 53 patients including 4 infants. they consisted of 30 cases of acute tonsillitis, 10 of peritonsillar abscess, 10 of laryngitis or pharyngitis, and 3 of sinusitis. 2. one of 2 g cmz was administered to an adult patient except for l case and 0.5 g to an infant pa ...19826752466
the use of an anaerobic incubator for the isolation of anaerobes from clinical samples.an anaerobic incubator was compared with a standard jar system for the isolation of anaerobes from clinical material. seventy specimens were selected as likely to yield anaerobes: 342 different anaerobes were isolated in the incubator and 347 in anaebrobic jars. these included bacteroides spp (43%), peptococcus spp (26%), peptostreptococcus spp (13%), veillonella spp (7%), fusobacterium spp (7%), clostridium spp (2%) and miscellaneous gram-positive nonsporing bacilli (2%). differences in isolati ...19826752210
vaginal organisms in prepubertal children with and without vulvovaginitis. a vaginoscopic study.in a prospective clinical and microbiological study covering 67 prepubertal children the vaginal flora of 31 asymptomatic children was compared to 36 children suffering from abnormal vaginal discharge and/or vulvovaginitis. vaginoscopy was employed to obtain bacteriological specimens without contamination. swabs were inoculated into prereduced transportmedia (port a cul, bbl) and cultured aerobically and anaerobically. the most prevalent aerobic organisms were staphylococcus epidermidis. enteroc ...19826751240
antibody reactive with peptostreptococcus micros in the sera of patients with periodontal disease.subgingival plaque samples were cultured for the isolation of peptostreptococcus micros from individuals with and without chronic periodontitis. humoral antibody from each person reacted with p. micros in an indirect fluorescent-antibody test, but not all of the sera reacted in a passive hemolysis test. no correlations were observed between the presence of antibody reactive with p. micros and the isolation of p. micros or the gingival health of the individual.19826749898
a note on fermentation reactions of anaerobic bacteria on a solid medium. 19826749787
pathogenicity of anaerobic gram-positive cocci.the pathogenicity of 20 strains of facultative or anaerobic gram-positive cocci (agpc) was investigated by injecting them alone or mixed with other flora into mice, utilizing the subcutaneous abscess model. abscesses induced by a mixture of two organisms were uniformly larger than those induced by single organisms. the relationships among seven agpc strains, eight aerobes, and two bacteroides spp. were determined by treating the infected animals with antibiotics and observing the effect of thera ...19846746091
radiological case of the month. anaerobic pyopneumopericardium. 19846741898
osteomyelitis in dogs and cats caused by anaerobic bacteria.localised osteomyelitis was diagnosed in 2 dogs and 2 cats. the disease was caused by fight wounds in 3 cases. radiographic examination demonstrated a circumscribed zone of cortical bone lysis, sequestra and periosteal new bone. each case was treated surgically by sequestrectomy and debridement. infection was due mainly to anaerobic bacteria. the pathogenic bacteria isolated from the lesions of dogs were actinomyces viscosus, fusobacterium nucleatum and bacteroides spp, and from the lesions in c ...19846732670
bactericidal and cytotoxic effects of hypothiocyanite-hydrogen peroxide mixtures.lactoperoxidase catalyzes the oxidation of thiocyanate by hydrogen peroxide into hypothiocyanite, a reaction which can protect bacterial and mammalian cells from killing by hydrogen peroxide. the present study demonstrates, however, that lactoperoxidase in the presence of thiocyanate can actually potentiate the bactericidal and cytotoxic effects of hydrogen peroxide under specific conditions, such as when hydrogen peroxide is present in the reaction mixtures in excess of thiocyanate. the toxic a ...19846724690
agglutinins to anaerobic bacteria in crohn's disease and in indian patients with diarrhoea.agglutinins to certain species of eubacterium and peptostreptococcus have been reported in sera from a high proportion of patients with crohn's disease. because this might be a non-specific finding common to patients with diarrhoea associated with damaged intestinal mucosa, we have compared the incidence of such agglutinins in patients with crohn's disease with that seen in patients in north-east india with acute or chronic diarrhoea. the incidence of agglutinins in crohn's disease was 44%, comp ...19846708086
in vitro activity of u-57930e against anaerobic bacteria and its comparison with clindamycin, ampicillin, carbenicillin and tetracycline.the in vitro activity of u-57930e, a pipecolic acid amide of clindamycin, was compared with those of clindamycin, ampicillin, carbenicillin and tetracycline against 321 anaerobic clinical isolates. the mic (micrograms/ml) of u-57930e that inhibited 95% bacteroides fragilis, peptococcus prevotii, b. melaninogenicus and p. asaccharolyticus was 0.0625; 0.03125 for peptostreptococcus anaerobius, b. vulgatus, propionibacterium and peptococcus species. clindamycin, on the other hand, gave mic values o ...19836678915
[clinical evaluation of tinidazole on anaerobic infections in the field of obstetrics and gynecology].the bacteriological and clinical effect of tinidazole (tdz) was evaluated in 16 cases of intrauterine, intrapelvic and vulvar infection caused by anaerobic organisms and the following results were obtained. anaerobes were detected in 16 cases, including 1 case with anaerobes alone and 15 cases with mixed anaerobes and aerobes. eight different species and 24 strains were detected. a single species was isolated from 9 cases, 2 species from 6 cases and 3 species from 1 case. the main species detect ...19836674545
selected bacterial antibodies in crohn's disease and ulcerative colitis.agglutinins to four strains of anaerobic gram-positive coccoid rods (species of eubacterium, peptostreptococcus and coprococcus) were found in significantly higher frequency in crohn's disease (cd) than in ulcerative colitis (uc) and in other diseased control subjects and were virtually absent in apparently healthy subjects. when the posterior probability of having cd was calculated on the basis of these agglutination reactions, 64% of patients with cd and 34% of patients with uc but only 10% of ...19836673050
the effect of lincomycin-neomycin treatment on experimental anaerobic bacterial bovine mastitis.three healthy lactating quarters of a friesland cow were each experimentally infected with a pure culture of a strain of either bacteroides fragilis, eubacterium lentum or a peptostreptococcus sp. respectively. the onset and progression to clinical mastitis was monitored 12 hourly by examination for clinical signs of inflammation, bacterial culture, somatic cell counts and with a strip cup. all infected quarters developed clinical mastitis within 24 hours. the 2 quarters infected with b. fragili ...19836668573
[clinical study of cefroxadine in the field of obstetrics and gynecology].clinical study of cefroxadine (cxd), an orally active cephalosporin, for the treatment of infections in the field of obstetrics and gynecology was carried out and the following results were obtained. fifteen strains were isolated in present study. these isolates were mainly e. coli and anaerobes (peptococcus sp., peptostreptococcus sp.). the distribution of susceptibilities to cxd of e. coli was between 6.25 approximately 12.5 micrograms/ml and that of anaerobes was between 0.39 approximately 1. ...19836655870
[experimental and clinical evaluation of cefroxadine in the field of obstetrics and gynecology].cefroxadine (cxd) was studied in the field of obstetrics and gynecology and the following results were obtained. mic values of cxd against 55 clinical isolates were investigated, from 6.25 to 12.5 micrograms/ml for 8 strains of e. coli, 6.25 micrograms/ml for 3 strains of k. pneumoniae and from 0.39 to 3.13 micrograms/ml for 9 strains of anaerobes such as peptostreptococcus and peptococcus in 10(6) cells/ml. transfer of cxd into intrapelvic organ such as uterus, uterine tube and ovary after the ...19836655868
death of an african elephant from probable toxemia attributed to chronic pulpitis.a 31-year-old captive male african elephant (loxodonta africana) of 5,000-kg body weight died suddenly in ventral recumbency. lesions seen at necropsy were bilateral purulent pulpitis and periodontitis of both tusks, serous atrophy of coronary groove fat, grammocephalus cholangitis, myocardial and skeletal lipofuscinosis, and scattered segmental necrosis in the pectoral muscles. nonhemolytic streptococci, corynebacterium sp, peptostreptococcus anaerobius, fusobacterium nucleatum, and bacteroides ...19836643242
tubo-ovarian abscess: contemporary approach to management.two hundred and thirty-two patients with tubo-ovarian abscesses (toas) were evaluated. ruptured toas were documented in seven (3%) of the patients. one hundred and seventy-five patients with toas were treated with antibiotics alone; for 15 of these patients, toas were confirmed by laparoscopy. the remaining 57 patients required surgical intervention: drainage (five patients), unilateral salpingo-oophorectomy (19) and total abdominal hysterectomy and bilateral salpingo-oophorectomy (33). a unilat ...19836635426
bacteriology of dental abscesses of endodontic origin.aspirates have been cultured from 10 dental abscesses of endodontic origin, all of which had penetrated beyond the bony alveolus to produce fluctuant swelling. sampling was by syringe aspiration. strict anaerobic techniques, including the use of an anaerobic chamber, were used for serial dilution and plating. randomly selected colonies (100) from each culture were purified, characterized, and identified. seventy percent of the bacterial isolates were either strict anaerobes or microaerophilic. o ...19836630460
an international study of agglutinins to eubacterium, peptostreptococcus and coprococcus species in crohn's disease, ulcerative colitis and control subjects.the world-wide occurrence of agglutinating antibodies to four coccoid anaerobes belonging to eubacterium, peptostreptococcus and coprococcus spp. was investigated in 937 coded sera from patients suffering from crohn's disease, ulcerative colitis, various other diseases and from healthy controls. positive results were found in 59% of patients with crohn's disease, 29% of patients with ulcerative colitis, and 8% of both diseased and healthy control subjects. patients with crohn's disease of the co ...19836628839
b-lymphocyte blastogenesis in response to periodontitis-associated bacteria. kinetics and proportion of total response.a role for activated b-lymphocytes in mediating the initiation and/or progression of periodontal diseases has been proposed in previous work. the present study was conducted to: (1) assess the proportion of total lymphocyte blastogenic response to selected oral bacteria which is composed of t-cell and b-cell activation, respectively, and (2) to determine whether different kinetic patterns exist for the t-cell vs. the b-cell responses to these bacteria. using lymphocyte rosetting and culturing te ...19846610740
gardnerella vaginalis and anaerobic bacteria in genital disease.in a study of gardnerella vaginalis and anaerobic bacteria in non-specific vaginitis (nsv) and other genital disease 89 patients attending a genital medicine clinic had vaginal samples examined for conventional pathogens and for quantitative analysis of g vaginalis and aerobic and anaerobic bacterial flora. the overall incidence of g vaginalis was 20%; g vaginalis (mean concentration 7.0 log10/g of secretion) occurred predominantly in patients with nsv (57%) but also in sexual contacts of non-sp ...19836600955
experimental otitis media in guinea pigs. serum antibody response to bacteroides fragilis, propionibacterium acnes and peptostreptococcus anaerobius upon challenge.the production of antibodies in serum was studied after inoculation of bacteroides fragilis, propionibacterium acnes or peptostreptococcus anaerobius into the middle ear of guinea pigs. inoculation of b. fragilis resulted in igg and igm serum antibodies at an early stage of the infection, while iga antibodies were seldom detected and at a later stage. in contrast, inoculation of p. acnes or p. anaerobius induced no serum antibody response. of these three species b. fragilis induced the most inte ...19846598260
[recently detected organisms from oral infectious diseases and their antimicrobial sensitivity]. 19846597502
phase variation of hyaluronidase-producing peptostreptococci associated with periodontal disease.peptostreptococci isolated from diseased periodontal pockets produced large amounts of extracellular hyaluronidase throughout their growth cycle. phase variants, obtained by re-streaking aged colonies of the wild types, had different colonial morphology and shorter generation times. these variants produced less hyaluronidase, and enzyme production occurred only during the early stationary phase.19836575993
purification and characterization of nadp-dependent 7 beta-hydroxysteroid dehydrogenase from peptostreptococcus productus strain b-52.an nadp-dependent 7 beta-hydroxysteroid dehydrogenase was purified 11.5-fold over the activity in crude cell extracts prepared from peptostreptococcus productus strain b-52, by using sephadex g-200 and deae-cellulose column chromatography. 7 beta-dehydrogenation was the sole transformation of bile acids catalyzed by the partially purified enzyme. the enzyme preparation (spec. act. 2.781 iu per mg protein) had an optimum ph of 9.8. lineweaver-burk plots showed a michaelis constant (km) value of 0 ...19836572075
[vertebral osteomyelitis caused by anaerobes. a new case]. 19846531485
suppurative peritonitis in cats associated with anaerobic bacteria. 19846529399
in vitro susceptibility of anaerobic bacteria to ciprofloxacin (bay o 9867).about 80% of 70 clinical isolates of bacteroides fragilis were inhibited by 4 micrograms of ciprofloxacin (bay o 9867) per ml. the 90% mic of ciprofloxacin was 8 micrograms/ml for other bacteroides species, 2 micrograms/ml for peptococcus species, 8 micrograms/ml for peptostreptococcus species, and 16 micrograms/ml for clostridium and eubacterium species.19846517561
intestinal water-soluble mucins in germfree, exgermfree and conventional animals.water-soluble intestinal mucins were investigated in germfree (gf), exgermfree (exg) and conventional (conv) rats and in gf and conv mice. after agar gel electrophoresis, all gf animals had similar specific band patterns demonstrated by pas and toluidine blue. these patterns, never seen in conv animals, disappeared in gf animals infected either with intestinal contents from conv rats or mono-infected with a mucin converting microorganism, labelled peptostreptococcus n. the intestinal microflora ...19846516849
characterization of peptostreptococcus asaccharolyticus glutamate dehydrogenase purified by dye-ligand chromatography.glutamate dehydrogenase (l-glutamate:nad+ oxidoreductase (deaminating); ec 1.4.1.2) has been purified from peptostreptococcus asaccharolyticus in a single step using dye-ligand chromatography. the enzyme (gdh) was present in high yields and was stabilized in crude extracts. a subunit molecular weight of 49000 +/- 500 was determined by sds polyacrylamide gel electrophoresis and six bands were obtained after cross-linking the subunits with dimethyl suberimidate. this bacterial gdh was predominantl ...19846502134
induction in rats of serum agglutinins to eubacterium, peptostreptococcus and coprococcus species by the faecal flora from patients with crohn's disease and healthy subjects.sera from patients with crohn's disease more often than those from other subjects contain agglutinins against anaerobic coccoid rods, identified as peptostreptococcus productus, eubacterium contortum (two strains) and coprococcus comes. the presence of antigens of the four coccoid rods in faeces of patients with crohn's disease and healthy subjects was investigated by inoculation of rats with faecal suspensions. antigens of the coccoid rods were detected in faeces from both patients with crohn's ...19846501880
microorganisms isolated from infected aural fistulas.aural fistula is a congenital deformity of the external ear relatively common in orientals and rare in caucasians. suppuration tends to occur, and chemotherapy rather than surgical drainage should be attempted. however, the lack of information concerning infected aural fistulas has made appropriate chemotherapy difficult. microorganisms isolated from 13 cases of infected aural fistulas were studied from january 1981 to december 1982. six species and 22 strains were isolated; nonsporeforming facu ...19846492970
peptostreptococcal empyema in akinetic mutism.peptostreptococcal empyema occurred in a 54-year-old man who had suffered from diabetes mellitus and had been in akinetic mutism. ultrasonic tomogram was useful to detect the space of empyema and to identify the decrease in volume of the empyema space with treatment.19846492491
anaerobic ureolytic bacteria from caecal content and soft faeces of rabbit.forty strains of ureolytic bacteria were isolated from the caecal content and soft faeces of seven rabbits by the anaerobic roll tube method and were characterized. the isolates were identified with clostridium coccoides, cl. innocuum, peptostreptococcus productus, p. micros, peptococcus magnus, fusobacterium russii and fusobacterium sp. urease activity of representative strains of the various species was also determined. the study indicated that strongly-ureolytic anaerobic bacteria are present ...19846490567
production of menaquinones by intestinal anaerobes.ninety intestinal organisms (71 isolates from fecal samples of neutropenic patients with cancer or from various sites in patients with intraabdominal infections and 19 control strains) were examined by reverse-phase thin-layer chromatography for their ability to produce menaquinones in vitro. menaquinones were found in all of 24 organisms of the bacteroides fragilis group. two other bacteroides species, bacteroides disiens and bacteroides bivius, also produced menaquinones. a single isolate of b ...19846470528
anaerobic bacteria isolated from foot infections in diabetic patients: in vitro susceptibility to nine antibiotics.twenty-nine isolates of anaerobic bacteria from foot infections in diabetic patients were tested, using the agar dilution method, for in vitro susceptibility to nine antimicrobial agents. imipenem and metronidazole showed excellent activity; clindamycin and chloramphenicol were next in terms of efficacy. cefoxitin and moxalactam demonstrated good activity, although three isolates of the bacteroides fragilis group were resistant to moxalactam. penicillin g, cefotaxime, and cefoperazone showed poo ...19846467279
surgical management of piriform sinus fistula.four cases of acute cervical abscess resulting from infection through the left piriform sinus fistula are described. the fistula seems to be related to the cause of infection in many of previously reported cases of acute suppurative thyroiditis or "recurrent lateral cervical fistula." complete removal of the fistula is essential to a permanent cure, and the guide of a fogarty catheter through the fistula is useful at operation for patients with repeated infection.19846465693
diffuse sclerosing osteomyelitis of the mandible.the medical care of patients with diffuse sclerosing osteomyelitis (dso) of the mandible has been unsatisfactory. the main reasons for this have been insufficient knowledge of the disease and its natural history, difficulties in establishing the correct diagnosis, and the unknown etiology. the clinical features and natural history of the disease were studied by means of repeated radiographic and scintigraphic recordings. the biopsy technique was improved by using a slowly rotating coarse trepan ...19846437998
[a case of septic pulmonary emboli due to peptostreptococcus associated with liver abscess]. 19846434659
peptostreptococcus productus strain that grows rapidly with co as the energy source.anaerobic bacteria were enriched with a sewage digestor sludge inoculum and a mineral medium supplemented with b-vitamins and 0.05% yeast extract and with a 50% co-30% n2-20% co2 (2 atm [202 kpa]) gas phase. microscopic observation revealed an abundance of gram-positive cocci, 1.0 by 1.4 micron, which occurred in pairs or chains. the coccus, strain u-1, was isolated by using roll tubes with co as the energy source. based on morphology, sugars fermented, fermentation products from glucose (h2, ac ...19846430231
cefamandole therapy in hand infections.hand lacerations, especially when inadequately treated, may result in infections caused by aerobic or anaerobic bacteria. anaerobic infections most commonly result from human bite injuries in which there is contact between hand and mouth. the search continues for an ideal antibiotic to employ when anaerobic organisms are suspected. in this study cefamandole, a new cephalosporin antibiotic, was employed whenever anaerobic hand infections were suspected following trauma. in each patient quantitati ...19836415153
susceptibility of brain to aerobic, anaerobic, and fungal organisms.the utility of an experimental animal model is dependent on its ability to simulate the actual clinical situation. with a stereotaxic injection procedure, the susceptibility of rat brain to the spectrum of organisms commonly associated with human brain abscess was determined. two strains of escherichia coli were more infective than pseudomonas aeruginosa, staphylococcus aureus, and streptococcus pyogenes. even between the e. coli strains it was possible to document significant differences in deg ...19836409808
[bacteriological and clinical studies in twenty patients with thoracic empyema--significance of anaerobic bacteria]. 19836408199
[anaerobic microflora of the contents of the upper maxillary sinuses in patients with chronic sinusitis]. 19846401097
isolation of obligate anaerobic bacteria from bovine abscesses in sites other than the liver.a survey in japan showed that of 2036 slaughtered cattle 58(3%) had abscesses in sites other than the liver. in 21 of the affected animals the lesions were pulmonary and in 32 abdominal (excluding hepatic); in five animals the lesions were found elsewhere (muscle 2, skin 2, bone 1). nineteen (33%) of the 58 cattle also had abscesses in the liver. obligate anaerobes alone were isolated from 17(29%) of the affected animals (fusobacterium necrophorum 14, bacteroides spp. 2, peptostreptococcus anaer ...19846389873
[growth of anaerobes of the upper small intestine using the glovebox technic].with 80 patients a microecologic analysis of the duodenal flora has been performed. due to the optimal cultivation technique with the glove-box more frequently as supposed till now, anaerobes with partial high numbers could be detected, and classified to different types of dysbiosis. a striking role among the isolated and classified gram-positive roots play the genera eubacterium, propionibacterium, actinomyces, and bifidobacterium. peptococcus and peptostreptococcus are to be found frequently, ...19846387500
clinical efficacy of cefoperazone in obstetric and gynecologic infections.compared with second-generation cephalosporins, cefoperazone, a third-generation cephalosporin, has demonstrated an extended antibacterial spectrum. in addition, it has a long serum half-life and requires only twice-a-day administration. in this study, 66 patients with clinically diagnosed obstetric and gynecologic infections were treated with cefoperazone, and 61 were evaluated for efficacy. an overall cure rate of 93% (57 patients) was achieved. in view of the excellent clinical response and c ...19846383613
pectinolytic enzymes of oral spirochetes from humans.five strains of obligately anaerobic, pectin-fermenting spirochetes were isolated from the subgingival plaque of humans. the strains produced two extracellular enzymatic activities that functioned in pectin degradation. one of these enzymatic activities was pectin methylesterase (ec 3.1.1.11), and the other was pectate lyase (ec 4.2.2.2) of the endo type. the data indicate that the cumulative action of these two enzymatic activities brought about depolymerization of pectin in spirochete cultures ...19846383218
anaerobes as normal oral flora.the human mouth provides a suitable habitat for numerous bacterial genera. anaerobic genera or genera that include anaerobic members found in the oral cavity are actinomyces, arachnia, bacteroides, bifidobacterium, eubacterium, fusobacterium, lactobacillus, leptotrichia, peptococcus, peptostreptococcus, propionibacterium, selenomonas, treponema, and veillonella. the incidence of anaerobes varies with age of the individual and with specific sites sampled. in edentulous infants, the incidence of a ...19846372039
role of anaerobic bacteria in bite-wound infections.the etiologic agents usually involved in wound infections due to human or animal bites are the aerobic skin flora of the victim, e.g., staphylococcus aureus, and/or the aerobic oral flora of the biter, e.g., pasteurella multocida. while anaerobic bacteria are predominant in the normal oral flora of humans and animals, their importance in the pathogenesis of bite-wound infections has not been stressed. most investigators in this field have either not cultured these wounds for anaerobic bacteria o ...19846372026
anaerobic oral and dental infection.anaerobes make up a significant part of the oral and dental indigenous and pathogenic flora. their role in periodontal disease, root canal infections, infections of the hard and soft oral tissue, as well as their importance as foci for disseminated infectious disease is well established. despite the ubiquitous involvement of bacteria, significant progress in our understanding of specific microbial etiologies has occurred only in the past decade. estimates of the number of species recovered from ...19846372018
effects of atmosphere of incubation on recovery of bacteria and yeasts from blood cultures in tryptic soy broth.a comparison was made of the results of blood cultures between 1974 and 1981 in unvented and transiently vented bottles of tryptic soy broth under vacuum with co2. a total of 14,646 isolates were available for statistical analysis. significantly more isolates of staphylococcus epidermidis, staphylococcus aureus, bacillus, escherichia, pseudomonas, klebsiella, serratia, acinetobacter, alcaligenes, neisseria, and candida were recovered from the vented bottle. significantly more isolates of coryneb ...19836370565
the frequency and some characteristics of anaerobic bacteria isolated from various forms of bovine mastitis.the prevalence of strictly anaerobic bacteria in the secretions from untreated cases of mastitis in lactating dairy cows was investigated. the study involved 147 friesland cows in 12 highveld herds. all herds yielded cows with anaerobic udder infections. no anaerobic bacteria were recovered from cows with normal quarters or those with latent aerobic infections. only anaerobes were present in 10% of so-called 'aseptic' mastitis cases. a variety of anaerobic organisms was isolated concurrently wit ...19836355474
continuous opacity monitoring of the growth of bacteria under strict anaerobic conditions.a newly designed instrument is described which generates continuous records of the opacities of six bacterial cultures growing under strict anaerobic conditions. additions (for example, of antibacterial agents) or withdrawal of culture (for example, for viable counting) can be made at any time without breach of anaerobiosis. use of the instrument is illustrated by growth curves obtained from small inocula of two strict anaerobes, bacteroides asaccharolyticus and peptostreptococcus anaerobius and ...19836355193
on the occurrence of enoate reductase and 2-oxo-carboxylate reductase in clostridia and some observations on the amino acid fermentation by peptostreptococcus anaerobius.enoate reductase present in clostridium kluyveri and clostridium spec. la 1 could be detected in three strains of c. tyrobutyricum and ten clostridia belonging to the groups of proteolytic and saccharolytic or proteolytic species, respectively. in c. pasteurianum, c. butyricum and c. propionicum enoate reductase could not be found even after growth on (e)-2-butenoate. a 2-oxo-carboxylate reductase was present in rather low activities in the non-proteolytic clostridia which produce enoate reducta ...19836354130
production of histolytic enzymes by a combination of oral bacteria with known pathogenicity.eight oral bacterial strains, isolated from an infected root canal, have been investigated for their capacity to produce histolytic enzymes. the determination was performed using methods espoused by two different principles. eleven out of 12 enzymes examined were demonstrated in the "eight-strain collection". in no single bacterial strain were all enzymes revealed. it was suggested that the pathogenicity of the bacterial strains, singly or in combination, was not solely dependent on the producti ...19836352755
the recovery of anaerobic bacteria from swabs in three transport systems. statistical evaluation by application of a variance component model.the recovery of six anaerobic species from swabs in three transport systems was quantitatively evaluated. the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum, and clostridium ramosum. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), or a plain cotton swab in either a dry aerobic tube (da) or an aerobic agar tube (aa). swabs were charged ...19836346790
the survival of anaerobic bacteria at 4 degrees c and 22 degrees c on swabs in three transport systems. statistical evaluation by application of a variance component model.the survival of anaerobic bacteria on swabs in three transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), a plain cotton swab in a dry aerobic tube (da) and a plain cotton swab in an aerobic agar tube (aa). the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum and clostridium ra ...19836346789
[use of cephalosporins as antibiotic prophylaxis in cesarean section].cefoxitin, a second-generation cephalosporin, was compared with cefazolin, a first-generation cephalosporin, and a placebo in a prospective, double-blind study of antibiotic prophylaxis in women undergoing nonelective cesarean section. in the groups that received cefazolin or the placebo there eas no statistically significant change in colonization of the cervix by aerobic bacteria by the fourth day after the operation, but there was a statistically significant increase in colonization by anaero ...19836344970
frequency of occurrence and antimicrobial susceptibility of bacterial isolates from the intestinal and female genital tracts.in the selection of empiric therapy for infections of the female genital tract and intraabdominal infections in humans, the requisite information includes (1) the frequency of isolation of bacterial species dominant in the normal gastrointestinal and female genital tracts and in intraabdominal and female genital tract infections and (2) the in vitro susceptibilities of the dominant species to drugs that may be used against them (e.g., penicillin g, cefoperazone, cefoxitin, chloramphenicol, clind ...19836342102
bacteriology of chronic tonsillitis in young adults.tonsils were obtained from 22 young adults (mean age, 23 years) suffering from chronic tonsillitis. mixed aerobic and anaerobic flora was obtained from core tonsillar cultures in all patients, yielding an average of 9.0 isolates (5.3 anaerobes and 3.7 aerobes) per specimen. the predominant anaerobic isolates were bacteroides sp, fusobacterium sp, and gram-positive cocci. the predominant aerobic isolates were alpha-hemolytic streptococci, staphylococcus aureus, branhamella catarrhalis, beta-hemol ...19846334513
ceftriaxone: therapeutic results in various infections and kinetic studies.ceftriaxone (ro 13-9904, rocephin) was given to 67 patients suffering from 74 various infections. patients had infections of the urinary tract (36), soft tissue phlegmon (12), infections of the respiratory tract (13), osteomyelitis (7), abscesses (5) and meningitis (1). infecting organisms were e. coli (26), proteus spp. (20), p. aeruginosa (7), h. influenzae (6), enterobacter spp. (6), k. pneumoniae (3), c. freundii (2), s. marcescens (1), s. aureus (4), s. pneumoniae (2), peptostreptococcus sp ...19846329238
[cefotiam passage into the maxillary sinus tissues].clinical study was made on cefotiam (ctm) and the following results were obtained. tissue concentrations of ctm were determined 1 hour after intravenous injection (ctm 1 g) in chronic sinusitis and maxillary cyst. concentrations of ctm were 16.53 micrograms/g, 13.26 micrograms/g in mucosa of the maxillary sinus, maxillary cyst, respectively. antibacterial activity of ctm was measured on escherichia coli, staphylococcus epidermidis, aerococcus, acidaminococcus fermentans, peptostreptococcus anaer ...19836325743
[clinical experience with cefotiam in otorhinolaryngologic infections].clinical studies of cefotiam (ctm), a new cephalosporin derivative, in otorhinolaryngological field were performed, and the results were summarized as follows. ctm was intravenously injected to 31 cases of otorhinolaryngological infections in daily dose of 1--4 g. clinical efficacy was 33.3% in acute otitis media and chronic otitis media (acute exacerbation) (6 cases), 90% in acute tonsillitis (including peritonsillitis, peritonsillar abscess) (20 cases), 50% in acute pharyngitis (2 cases), 100% ...19836325742
inactivation of the polyanionic detergent sodium polyanetholsulfonate by hemoglobin.sodium polyanetholsulfonate (sps) has been added to blood culture media for many years. its incorporation results in a higher yield of positive blood cultures due to its inactivation of antimicrobial cationic compounds. the most active of these cations include complement components, aminoglycoside-aminocyclitol antibiotics, and receptors on polymorphonuclear leukocytes. there have been reports from studies conducted outside patient blood culture bottles that sps itself may possess antibacterial ...19836315765
cecal microflora of turkeys fed low or high fiber diets: enumeration, identification, and determination of cellulolytic activity.examination of cecal contents or bacterial cultures thereof from turkeys fed either a high fiber (hf) or low fiber (lf) ration indicated that direct microscopic counts of microbes were significantly higher in hf-fed than in lf-fed birds. there was no significant difference in mean colony counts between the two groups of turkeys. in both lf and hf-fed birds, 77% of the microbes were gram-positive rods, 14% gram-negative rods, and 9% gram-positive cocci. the predominant microorganism was eubacteri ...19836306633
[clinical bacteriological studies on cefotiam and cefsulodin in the field of otorhinolaryngology].clinical bacteriological studies on cefotiam and cefsulodin in the field of otorhinolaryngology were carried out and the following results were obtained. 1) aerobic and anaerobic gram-positive bacteria were dominantly isolated from the clinical materials sent to the center from the clinical institutes. 2) it was considered that streptococcus pneumoniae, haemophilus influenzae and beta-streptococcus played an important role in the primary infections in the field of otorhinolaryngology. staphyloco ...19826290705
[a fundamental and clinical study of ceftizoxime in the field of obstetrics and gynecology (author's transl)].a fundamental and clinical study of ceftizoxime (czx), a new cephalosporin antibiotic, has brought about the following results. 1. the antibacterial activity of czx against bacteriodes fragilis and b. thetaiotaomicron was superior to that of cez, but inferior to that of cfx. czx was the most active of the 3 drugs against b. distasonis, and was as active as cfx against b. vulgatus. the mics of czx against peptococcus, peptostreptococcus and anaerobic streptococcus were 1.56 microgram/ml or lower. ...19816278175
[comparison of antibacterial activity of cefmenoxime with other cephalosporins against clinically isolated bacteria (author's transl)].we examined the antibacterial activity of mx in comparison with those of other ceps, using aerobic gram-positive cocci, aerobic gram-negative bacilli and anaerobic bacteria, 870 strains in total, all isolated from clinical specimens, in 1979 and 1980. against streptococcus, cmx showed superior antibacterial activity than those of cfx, cmz, cxm and ctm. against h. influenzae, cmx also showed superior antibacterial activity than those of cfx, cmx, cxm, ctm and cez. abpc-and pipc-resistant strains ...19816278174
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