Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
evaluation of bacteriophages during the treatment of sludge.the aim of this work was to determine the effect of liming and composting on the fate of three bacteriophages (somatic coliphages, f-rna phages, bacteroides fragilis phages) considered as potential indicators of viral contamination. it was shown that the three bacteriophages studied exhibited variable densities in sludge. somatic coliphages were most abundant (10(4) to 10(5) x 10 g(-1) dm) then f-rna bacteriophages (10(2) to 10(4) x 10 g(-1) dm) and bacteroides fragilis phages (10(1) to 10(2) x ...200212479470
[enterotoxigenic bacteroides fragilis as a factor in diarrhea].the etiology of almost half of the diarrheal diseases has not been cleared yet, in spite of modern diagnostic methods. bacteroides fragilis strains which secrete an enterotoxin are termed as enterotoxigenic b.fragilis (etbf). these strains are associated with diarrheal diseases in children above 1 year of age and in adults. b. fragilis toxin (bft) stimulates intestinal secretion and in-vitro cytotoxic response in ht29/c1 cells. recent studies suggest that bft is related to inflammatory bowel dis ...200212476774
increase in resistance to new fluoroquinolones from 1998 to 2001 in the bacteroides fragilis group.susceptibility to anti-anaerobic agents in the bacteroides fragilis group varies according to the geographical region studied. in recent years there has been a reduction in the susceptibility of such isolates, especially to certain antibiotics such as clindamycin and cefoxitin. the antimicrobial susceptibilities of 200 strains of the b. fragilis group isolated in 1998 (100) and 2001 (100) from faecal samples of healthy people to anti-anaerobic agents were determined. meropenem, metronidazole and ...200212461033
microbiology of polymicrobial abscesses and implications for therapy.abscesses that develop as a result of introduction of the normal flora into a normally sterile body site are often polymicrobial. this review summarizes past studies published by our group on the microbiology of polymicrobial abscesses that occur at various body sites. staphylococcus aureus and group a beta-haemolytic streptococci are the most prevalent aerobes in skin and soft tissue abscesses and are isolated at all body sites. in contrast, organisms that colonize the mucous membranes predomin ...200212460997
phages of enteric bacteria in fresh water with different levels of faecal pollution.levels of somatic and f-specific coliphages, and phages infecting bacteroides fragilis were measured in 257 samples collected in different freshwater environments with different levels and characteristics of faecal pollution. in samples with recent pollution of domestic origin, the numbers of the three groups of phages were highly correlated, thus showing that their excretion is fairly constant. in this set of samples somatic coliphages, which were the most abundant, and f-specific coliphages ou ...199712455890
microbial source tracking: current methodology and future directions. 200212450798
occurrence and levels of indicator bacteriophages in bathing waters throughout europe.somatic coliphages, f-specific rna bacteriophages, bacteriophages infecting bacteroides fragilis, escherichia coli and enterococci were counted in bathing waters in the late spring and summer. we tested fresh and marine bathing waters from north, south, east and west europe expected to contain between 100 and 500 e. coli per 100 ml, although wider ranges were sometimes found. bacteriophages were counted after concentration, since a preliminary study proved that this step was necessary to obtain ...200212448544
zwitterionic polysaccharides stimulate t cells by mhc class ii-dependent interactions.polysaccharides of pathogenic extracellular bacteria commonly have negatively charged groups or no charged groups at all. these molecules have been considered classic t cell-independent ags that do not elicit cell-mediated immune responses in mice. however, bacterial polysaccharides with a zwitterionic charge motif (zpss), such as the capsular polysaccharides of many strains of bacteroides fragilis, staphylococcus aureus, and streptococcus pneumoniae type 1 elicit potent cd4(+) t cell responses ...200212444118
effect of a single percutaneous abscess drainage puncture and imipenem therapy, alone or in combination, in treatment of mixed-infection abscesses in mice.the importance of supplementary imipenem therapy after a single percutaneous abscess drainage puncture was studied in a mouse model of established mixed-infection abscesses. animals were treated for 3 days with daily dosing regimens of 384 to 1,536 mg/kg of body weight that took into account the short half-life of this antibiotic in mice. imipenem therapy in conjunction with abscess drainage was significantly better than drainage alone in reducing the escherichia coli and bacteroides fragilis co ...200212435666
antianaerobic activity of moxifloxacin compared with that of ofloxacin, ciprofloxacin, clindamycin, metronidazole and beta-lactams.minimal inhibitory concentrations of moxifloxacin were compared with those of ofloxacin, ciprofloxacin, clindamycin, metronidazole and six beta-lactams for 159 anaerobes isolated from human clinical samples. unlike other fluoroquinolones, moxifloxacin demonstrated high activity against the 76 strains of the bacteroides fragilis group as the minimal inhibitory concentration(50) was 0.5 mg/l. porphyromonas, prevotella, fusobacterium and gram-positive anaerobic cocci were inhibited by 1 mg/l or les ...200212431872
necrotising infections of soft tissues--a clinical profile.to study the clinical profile and outcome of patients with necrotising soft-tissue infections.200212428876
acute mediastinal widening.mediastinal abscess resulting from descending necrotizing mediastinitis is a rare infectious process. odontogenic infections are the most commonly implicated underlying process. descending necrotizing mediastinitis is a rapidly progressive infectious process that spreads through the fascial planes of the neck to gain access to the mediastinum. early recognition of descending necrotizing mediastinitis is important because the reported mortality rate is 30% to 50% even in the antibiotic era. clues ...200212425517
in vitro susceptibilities of the bacteroides fragilis group species: change in isolation rates significantly affects overall susceptibility data.a comparison of antimicrobial susceptibility data of species of the bacteroides fragilis group for 1989-1990 and 1998-1999 studies showed statistically significant increases or decreases in in vitro activity. overall significant increases in resistance were noted for ampicillin-sulbactam and clindamycin, while significant decreases in resistance were noted for ertapenem and cefoxitin. susceptibilities to piperacillin-tazobactam, imipenem, meropenem, and trovafloxacin remained virtually the same ...200212409429
development of 16s rrna-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces.for the detection and identification of predominant bacteria in human feces, 16s rrna-gene-targeted group-specific primers for the bacteroides fragilis group, bifidobacterium, the clostridium coccoides group, and prevotella were designed and evaluated. the specificity of these primers was confirmed by using dna extracted from 90 species that are commonly found in the human intestinal microflora. the group-specific primers were then used for identification of 300 isolates from feces of six health ...200212406736
joint and bone infections due to anaerobic bacteria in children.the current review describes the microbiology, diagnosis and management of septic arthritis and osteomyelitis due to anaerobic bacteria in children. staphylococcus aureus, haemophilus influenzae type-b, and group a streptococcus, streptococcus pneumoniae, kingela kingae, neisseria meningiditis and salmonella spp are the predominant aerobic bacteria that cause arthritis in children. gonococcal arthritis can occur in sexually active adolescents. the predominant aerobes causing osteomyelitis in chi ...200212396847
molecular dynamics simulations of the dinuclear zinc-beta-lactamase from bacteroides fragilis complexed with imipenem.herein, we present results from md simulations of the michaelis complex formed between the dizinc beta-lactamase from b. fragilis and imipenem. we considered two catalytically important configurations, which differ in the presence or absence of a hydroxide bridge connecting the two zinc ions in the active site. the structural and dynamical effects induced by substrate binding, the specific roles of the conserved residues and the zinc-bound water molecules, the near attack conformers of the micha ...200212395427
conservation of phage reference materials and water samples containing bacteriophages of enteric bacteria.the survival was determined in different conservation conditions of: somatic coliphages, f-specific rna bacteriophages and phages infecting bacteroides fragilis proposed as model micro-organisms for water quality control. titres of phages of all groups either in pure culture phage suspensions or in naturally occurring phage suspensions were stable at (-70+/-10) degrees c and at (-20+/-5) degrees c when protected with glycerol. moreover, phage analysis of stored suspensions demonstrated that thei ...200212393152
recognition of anaerobic bacterial isolates in vitro using electronic nose technology.use of an electronic nose (e.nose) system to differentiation between anaerobic bacteria grown in vitro on agar media.200212390482
cefditoren, a new aminothiazolyl cephalosporin.cefditoren pivoxil, an oral third-generation cephalosporin, was approved by the food and drug administration in september 2001. it has been used in japan for several years. the greatest therapeutic potential of cefditoren appears to be its activity against gram-positive and gram-negative organisms causing respiratory tract infections and skin and skin-structure infections, such as haemophilus influenzae, streptococcus pneumoniae, and moraxella catarrhalis. cefditoren is also effective against me ...200212389878
effects of laparoscopic lavage on adhesion formation and peritoneum in an animal model of pelvic inflammatory disease.to develop an animal model of pelvic inflammatory disease (pid) that simulates intraluminal tubal damage and adhesion formation seen in human pid, and to evaluate peritoneal effects of laparoscopic lavage with 1% povidone-iodine, 0.5% povidone-iodine, and 0.05% chlorhexidine gluconate solutions.200212386364
in vitro activities of faropenem against 579 strains of anaerobic bacteria.the activity of faropenem, a new oral penem, was tested against 579 strains of anaerobic bacteria by using the nccls-approved reference method. drugs tested included amoxicillin-clavulanate, cefoxitin, clindamycin, faropenem, imipenem, and metronidazole. of the 176 strains of bacteroides fragilis group isolates tested, two isolates had faropenem mics of 64 micro g/ml and imipenem mics of >32 micro g/ml. faropenem had an mic of 16 micro g/ml for an additional isolate of b. fragilis; this strain w ...200212384389
a multiplex polymerase chain reaction-based diagnostic method for bacterial vaginosis.to develop a polymerase chain reaction (pcr)-based diagnostic method for bacterial vaginosis using bacterial vaginosis-associated anaerobes.200212383546
endocarditis due to anaerobic bacteria.this review describes the microbiology, diagnosis and management of endocarditis due to anaerobic bacteria. anaerobic bacteria are an uncommon but important cause of endocarditis. most cases of anaerobic endocarditis are caused by anaerobic cocci, propionibacterium acnes and bacteroides fragilis group. predisposing factors and signs and symptoms of endocarditis caused by anaerobic bacteria are similar to those seen in endocarditis with facultative anaerobic bacteria with the following exceptions ...200212373039
[a comparative study of the various organisms causing salpingitis and their potential presence in iud wearers].a prospective study was undertaken to compare the enbdometrial and vaginal bacteria of a continuous series of 283 patients who had iuds removed between june 1, 1979 and june 1, 1983, by the same clinician. measures were taken to avoid contamination of the iud upon removal by the vaginal bacteria, but as a practical matter it was impossible to avoid all contact. the iuds involved were multiload copper 250 or 375, gyne t and t 200, nova t, and dimelys. the number of positive cultures from the ...198512339972
nuclear factor-kappa b activation pathway in intestinal epithelial cells is a major regulator of chemokine gene expression and neutrophil migration induced by bacteroides fragilis enterotoxin.although intestinal epithelial cells are known to up-regulate the expression of several chemokine genes in response to the stimulation with b. fragilis enterotoxin (bft), there has been little understanding on the cellular mechanisms of bft-induced mucosal inflammation. to test whether nuclear transcriptional factor-kappa b (nf-kappab) is involved in the process, we stimulated intestinal epithelial cells with bft, and evaluated the signalling nf-kappab pathways. bft increased signals of nf-kappa ...200212296854
[open comparative study between the combination clyndamicin/gentamycin and penicillin/gentamycin in septic abortion].septic abortion is not uncommon in countries where abortion is illegal, and antibiotics are second only to uterine evacuation in the treatment of such cases. although the combination of penicillin and gentamycin has given excellent results, the high incidence of bacteroides fragilis and other anerobics in septic abortion prompted a comparison of penicillin and gentamycin with clindamycin and gentamycin at the hospital santo tomas in panama city, panama, beginning in may 1984. among 30 febrile ...198512280266
activity of endodontic antibacterial agents against selected anaerobic bacteria.the antimicrobial activity of substances used as antibacterial agents (solutions of 10% calcium hydroxide, camphorated paramonochlorophenol - pmcc, 2% chlorhexidine digluconate and 10% castor oil plant detergent) on anaerobic bacteria (fusobacterium nucleatum atcc 25586, prevotella nigrescens atcc 33563, clostridium perfringens atcc 13124 and bacteroidesfragilis atcc 25285), using a broth dilution technique, was evaluated in vitro. for determination of minimum inhibitory and minimum bactericide ...200212238802
in vitro activities of newer quinolones against bacteroides group organisms.the activities of bms-284576, clinafloxacin, moxifloxacin, sitafloxacin, trovafloxacin, imipenem, cefoxitin, and clindamycin against 589 bacteroides fragilis group isolates were determined. the activity of bms-284576 was comparable to that of trovafloxacin. sitafloxacin and clinafloxacin were the most active quinolones, and moxifloxacin was the least active. b. fragilis was the most susceptible of the species, and bacteroides vulgatus was the most resistant. association of specific antibiotic re ...200212234859
fates of bacteriophages and bacterial indicators in the moselle river (france).it has been suggested that bacteriophages can provide useful information about the pathogenic microorganisms, particularly enteric viruses, present in water. this information is complementary to that obtained from bacterial indicators of faecal contamination, which would be of great value for evaluating the risks associated with the use of certain types of water. before bacteriophages can be used as indicators of faecal contamination, we need to confirm that bacteriophages give a different respo ...200212230209
binding of d- and l-captopril inhibitors to metallo-beta-lactamase studied by polarizable molecular mechanics and quantum mechanics.the bacterial zn2+ metallo-beta-lactamase from b. fragilis is a zinc-enzyme with two potential metal ion binding sites. it cleaves the lactam ring of antibiotics, thus contributing to the acquired resistance of bacteria against antibiotics. the present study bears on the binuclear form of the enzyme. we compare several possible binding modes of captopril, a mercaptocarboxamide inhibitor of several zinc-metalloenzymes. two diastereoisomers of captopril were considered, with either a d- or an l-pr ...200212210153
environmental factors influencing human viral pathogens and their potential indicator organisms in the blue mussel, mytilus edulis: the first scandinavian report.this study was carried out in order to investigate human enteric virus contaminants in mussels from three sites on the west coast of sweden, representing a gradient of anthropogenic influence. mussels were sampled monthly during the period from february 2000 to july 2001 and analyzed for adeno-, entero-, norwalk-like, and hepatitis a viruses as well as the potential viral indicator organisms somatic coliphages, f-specific rna bacteriophages, bacteriophages infecting bacteroides fragilis, and esc ...200212200309
toll-like receptor 4 (tlr4)-deficient murine macrophage cell line as an in vitro assay system to show tlr4-independent signaling of bacteroides fragilis lipopolysaccharide.bacterial lipopolysaccharides (lps) activate cells of innate immunity, such as macrophages, by stimulating signaling through toll-like receptor 4 (tlr4). we and others have hypothesized that lps derived from different bacterial species may function through tlr4-independent mechanisms. to test this hypothesis, we have generated using a nonviral transformation procedure a bone marrow-derived macrophage cell line called 10scncr/23 from mouse strain c57bl/10scncr. this mouse strain has a deletion of ...200212183533
high incidence of cefoxitin and clindamycin resistance among anaerobes in taiwan.susceptibilities to 16 antimicrobial agents were determined by measurement of mics for 344 isolates of anaerobic bacteria recovered from patients with significant infections. resistance rates varied among antimicrobial agents and the species tested. the beta-lactams were more active in gram-positive than in gram-negative anaerobes. resistance to meropenem was low (<1%). for beta-lactam-beta-lactamase inhibitors, piperacillin-tazobactam was most active for all species (resistance, <6%). the rates ...200212183246
modulation of bft expression by the bacteroides fragilis pathogenicity island and its flanking region.to establish a recombinant system for high-level expression of biologically active bacteroides fragilis toxin (bft), we studied the expression of bft in non-toxigenic b. fragilis (ntbf) strains. the bft gene and the b. fragilis pathogenicity island (bfpai) were cloned into ntbf strains with two distinct genetic patterns: (i) pattern ii, strains lacking the bfpai and its flanking region; and (ii) pattern iii, strains lacking the bfpai but containing its flanking region. analysis of bft activity o ...200212180925
peritoneal infection: a possible redox disease.in a recent report, pertaining to bacteroidesfragilis peritonitis, the influence of oxidation-reduction (redox) potential provided experimental evidence for b. fragilis penetration into hela cell monolayers (using 3d imaging techniques). bacteria grown under oxidizing conditions (+mv redox) penetrated into tissue cells unlike that of reducing conditions (-mv redox). the present results emphasise the significance of the level of redox potential during infection with an interpretation based on ana ...200212173778
national survey on the susceptibility of bacteroides fragilis group: report and analysis of trends for 1997-2000.the results of a multicenter us survey using the national committee for clinical laboratory standards currently recommended methodology for measuring in vitro susceptibility of 2673 isolates of bacteroides fragilis group species were compared from 1997 to 2000. the test panel consisted of 14 antibiotics: 3 carbapenems, 3 beta-lactam-beta-lactamase inhibitors, 3 cephamycins, 2 fluoroquinolones, clindamycin, chloramphenicol, and metronidazole. declines in the geometric mean minimum inhibitory conc ...200212173121
anaerobic infections in the surgical patient: microbial etiology and therapy.anaerobic infections occur in surgical patients in part because of structural or functional defects in the host that (1) cause a breech in the normal mucosal barriers, (2) create localized vascular insufficiencies, or (3) produce an obstruction. any or all of these events may compromise the oxidation-reduction potential within the tissues, encouraging rapid anaerobic growth. although diverse anaerobic populations are spread throughout the gastrointestinal tract, a relatively limited number of or ...200212173119
intra-abdominal anaerobic infections: bacteriology and therapeutic potential of newer antimicrobial carbapenem, fluoroquinolone, and desfluoroquinolone therapeutic agents.intra-abdominal infections are biphasic, synergistic processes with early peritonitis and bacteremia due to aerobes and a later abscess component due to anaerobes. although bacteroides fragilis is the most commonly recognized pathogen, other anaerobes, including other members of the b. fragilis-group species, are major components of infection. anaerobic bacteremia is often associated with an intra-abdominal source. new antimicrobial agents with anaerobic activity are in various stages of develop ...200212173118
outer-membrane pore-forming proteins in gram-negative anaerobic bacteria.the outer-membrane proteins (omps) of bacteria function as the dynamic interface between the bacterium and its surroundings and are involved in maintenance of cell structure, binding a variety of substances, adhesion to other cells, and regulation of transport of both nutrients and bactericidal agents. there is a vast amount of information about aerobic omps and their roles in immunogenicity, virulence, and antimicrobial resistance. knowledge about omps in anaerobic bacteria is much sparser. gen ...200212173111
selection of cefoxitin-resistant bacteroides thetaiotaomicron mutants and mechanisms involved in beta-lactam resistance.the beta-lactam antibiotics are the most widely used of all the groups of antimicrobials, but beta-lactam resistance is increasingly common among members of the bacteroides fragilis group. three major mechanisms are involved in beta-lactam resistance, and they act together in certain instances. in the present study, 2 resistant mutants (238m and 1186m) of bacteroides thetaiotaomicron, obtained from clinical isolates (238 and 1186) by selection with increasing concentrations of cefoxitin, showed ...200212173108
recent taxonomic changes and terminology update of clinically significant anaerobic gram-negative bacteria (excluding spirochetes).because of access to 16s rdna sequencing, changes in the taxonomy and nomenclature of anaerobic gram-negative bacteria have occurred lately. new genera and species have been described, and existing taxa have been reclassified. the present article compiles a list of clinically relevant anaerobes and provides synonyms as well as the old nomenclature used for these bacteria. although names and classifications of anaerobic bacteria are changing quickly, it is important to keep track of new bacterial ...200212173103
design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples.an oligonucleotide-microarray method was developed for the detection of intestinal bacteria in fecal samples collected from human subjects. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to glass slides. cyanine5 (cy5)-labeled 16s rdnas were amplified by polymerase chain reaction (pcr) from human fecal samples or ...200212167534
structural study on lipid a and the o-specific polysaccharide of the lipopolysaccharide from a clinical isolate of bacteroides vulgatus from a patient with crohn's disease.bacteroides vulgatus has been shown to be involved in the aggravation of colitis. previously, we separated two potent virulence factors, capsular polysaccharide (cps) and lipopolysaccharide (lps), from a clinical isolate of b. vulgatus and characterized the structure of cps. in this study, we elucidated the structures of o-antigen polysaccharide (ops) and lipid a in the lps. lps was subjected to weak acid hydrolysis to produce the lipid a fraction and polysaccharide fraction. lipid a was isolate ...200212153568
cross-infection due to imipenem-resistant bacteroides fragilis associated with a totally implantable venous port.four patients in an oncology ward developed bacteroides fragilis bacteremia over a 12-day period. cross-infection between two of them, due to an imipenem-resistant strain, was demonstrated by epidemiological investigation and genotypic typing methods (arbitrarily primed pcr fingerprinting and nucleotide sequencing of the cfia genes and upstream is1186/is1168 elements).200212149371
evaluation of a new etest for detecting metallo-beta-lactamases in routine clinical testing.several etest (ab biodisk, solna, sweden) gradient formats were developed for detection of metallo-beta-lactamases based on the reduction of imipenem (ip) or ceftazidime (tz) mics in the presence of edta or 2-mercaptopropionic acid (mpa). the etest metallo-beta-lactamase (etest mbl) strips consisted of a double-sided seven-dilution range of ip or tz (4 to 256 microg/ml) and ip or tz (1 to 64 microg/ml) overlaid with a constant concentration of edta or mpa. the prototype strips were evaluated on ...200212149325
clinical review: bacteremia caused by anaerobic bacteria in children.this review describes the microbiology, diagnosis and management of bacteremia caused by anaerobic bacteria in children. bacteroides fragilis, peptostreptococcus sp., clostridium sp., and fusobacterium sp. were the most common clinically significant anaerobic isolates. the strains of anaerobic organisms found depended, to a large extent, on the portal of entry and the underlying disease. predisposing conditions include: malignant neoplasms, immunodeficiencies, chronic renal insufficiency, decubi ...200212133179
septic arthritis of the hip joint in cervical cancer patients after radiotherapy: three case reports.cervical cancer patients may experience hip problems related to the cancer itself or therapeutic management for the cancer. septic arthritis should be one of the possibilities but there have been no reports on this. here we present three patients who developed hip problems more than two years after radiotherapy with or without a radical operation. one patient was managed as septic arthritis because of significant inflammatory signs around the affected hip joint even though the causative organism ...200112118130
in-vitro activity of ciprofloxacin combined with flomoxef against bacteroides fragilis, compared with that of ciprofloxacin combined with clindamycin.using checkerboard and time-kill assays, the in-vitro activity of ciprofloxacin alone and in combination with flomoxef against clinical bacteroides fragilis strains was evaluated. in addition, the microbiological efficacy of this combination was compared with that of ciprofloxacin plus clindamycin. in 88% of the 25 strains tested, the combination of ciprofloxacin plus flomoxef exhibited a synergistic or an additive effect, whereas only 56% of the 25 strains ( p< 0.01, chi(2) test) tested with th ...200212111577
changes in the intestinal flora after the administration of prophylactic antibiotics to patients undergoing a gastrectomy.changes in the intestinal flora were investigated in patients administered antibiotics for 4 days after a gastrectomy.200212111513
stent graft infection after abdominal aortic aneurysm repair: a case report.a 77-year-old man had clinical and radiologic signs of graft infection develop 1 year after stent grafting for abdominal aortic aneurysm. blood cultures grew bacteroides fragilis, and cultures of the aneurysm sac grew enterococcus. the patient's condition was successfully managed with staged extraanatomic revascularization followed by graft excision. although stent graft infection to date is extremely rare, some aspects peculiar to the placement of these devices potentially could increase their ...200212096277
crystal structure of a transcarbamylase-like protein from the anaerobic bacterium bacteroides fragilis at 2.0 a resolution.a transcarbamylase-like protein essential for arginine biosynthesis in the anaerobic bacterium bacteroides fragilis has been purified and crystallized in space group p4(3)2(1)2 (a=b=153.4 a, c=94.8 a). the structure was solved using a single isomorphous replacement with anomalous scattering (siras) and was refined at 2.0 a resolution to an r-factor of 20.6% (r-free=25.2%). the molecular model is trimeric and comprises 960 amino acid residues, two phosphate groups and 422 water molecules. the mon ...200212095263
a novel mechanism for the regulation of amyloid precursor protein metabolism.modifier of cell adhesion protein (moca; previously called presenilin [ps] binding protein) is a dock180-related molecule, which interacts with ps1 and ps2, is localized to brain areas involved in alzheimer's disease (ad) pathology, and is lost from the soluble fraction of sporadic alzheimer's disease (ad) brains. because ps1 has been associated with gamma-secretase activity, moca may be involved in the regulation of beta-amyloid precursor protein (app) processing. here we show that the expressi ...200212093789
[bactericidal activity of trovafloxacin and moxifloxacin against gram-negative anaerobic bacilli].anaerobes are often involved in human pathology, including oral infections. the therapy of anaerobic infection requires certain antibiotics such as: metronidazole, tetracycline or amoxicillin. but the incidence of resistant strains among anaerobes is continuously increasing and it is necessary to find some new agents effective against these bacteria. the aim of our study was to establish the bactericidal activity of two new quinolones against 14 strains of gram-negative anaerobic bacilli. the mi ...200012089939
in vitro bactericidal activity of piperacillin, gentamicin, and metronidazole in a mixed model containing escherichia coli, enterococcus faecalis, and bacteroides fragilis.an anaerobic, mixed model assay was used to study the bactericidal activities of piperacillin, gentamicin, and metronidazole, alone and in double- and triple-antibiotic combinations against a polymicrobial suspension of e. coli, e. faecalis, and b. fragilis. only slight differences were noted with the agents when tested against single (10(5) cfu/ml inoculum) versus polymicrobic suspensions (10(6) cfu/ml final inoculum) of susceptible and resistant organisms. contrary to previous reports in the l ...200212088623
depuration dynamics of viruses in shellfish.the consumption of shellfish has been associated with viral infections even in cases where shellfish complied with the current regulation, which is based on bacterial analysis. in this study, depuration rates of potential indicators and human viruses have been analysed in order to study the use of complementary parameters for evaluating the microbiological quality of depurated shellfish. depuration of naturally highly polluted mussels has been evaluated and analyses for escherichia coli, clostri ...200212076030
[yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2000--ii. gram-negative bacteria].the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2000 were yearly evaluated and compared with those of other cephems, oxacephems, and carbapenems. thirty-two species 2,697 strains of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis (n = 125), escherichia coli (n = 250), citrobacter freu ...200212071094
bacteroides fragilis and escherichia coli bacteriophages in human faeces.some bacteriophages found in human faeces are being evaluated as possible indicators of viral contamination of water. these bacteriophages include somatic coliphages and bacteroides fragilis phages. the aims of this study were to determine the occurrence and concentrations of somatic coliphages and bacteroides fragilis phages in the stools of a human population residing in eastern france (n = 193). somatic coliphages were detected in 68% of the stools at a mean concentration of 4.3 x 10(3) pfu.g ...200212068752
synthesis and utility of sulfated chromogenic carbohydrate model substrates for measuring activities of mucin-desulfating enzymes.a chromogenic substrate, 4-nitrophenyl 2-acetamido-2-deoxy-beta-d-glucopyranoside 6-sodium sulfate was synthesized and used in combination with beta-n-acetylhexosaminidase for detection of the sulfatase, mdsa, by release of 4-nitrophenol. mdsa was originally isolated from the bacterium prevotella strain rs2 and is believed to be involved in desulfation of sulfomucins, major components of the mucus barrier protecting the human colon surface. the exo nature of the mdsa sulfatase was indicated by i ...200212062525
reclassification of bacteroides forsythus (tanner et al. 1986) as tannerella forsythensis corrig., gen. nov., comb. nov.the characteristics of the fusiform species bacteroides forsythus, isolated from human periodontal pockets, were examined. 165 rdna sequence analysis confirmed that b. forsythus was not a species within the genus bacteroides sensu stricto. although b. forsythus was phylogenetically related to bacteroides distasonis and bacteroides merdae in the phylogenetic tree, the ratio of anteiso-15:0 to iso-15:0 in whole-cell methanolysates of b. forsythus was different from those of b. distasonis, b. merda ...200212054248
primary psoas abscess. report of three cases.primary psoas abscesses are a rare clinical entity with subtle and non specific symptoms, most commonly seen in patients predisposed to infections. early diagnosis and appropriate management are therefore challenging aspects for physicians.200212051083
antibiotics and expression of microbial virulence factors: implications for host defense.the discovery that antibiotics are capable of modifying both the structural appearance and physiology of pathogenic bacteria when they are incorporated in the culture medium at sub-growth inhibitory concentrations, has led to numerous examples in which the expression of one or more virulence factors (both structural and soluble) is inhibited or potentiated--some of these bacterial products are recognized in their own right as substances which can interfere with one or more stages of the normal p ...199112041741
prevalence of gram negative anaerobic bacilli in routine clinical specimens.a total of 206 gram negative anaerobic bacilli were isolated from 2591 routine clinical specimens. majority of them (78.2%) were part of a mixed flora. a high incidence of gram negative anaerobic bacilli was found in post-operative wound infections and diabetic foot infections (30.1%), followed by necrotizing fasciitis and crush injuries (18%), pus of empyema and lung abscesses (14.5%) and aspirates from other deep abscesses (10.7%). bacteroides fragilis was the most frequently recognized specie ...200112035357
molecular cloning and expression in escherichia coli of an exo-levanase gene from the endophytic bacterium gluconacetobacter diazotrophicus srt4.gluconacetobacter diazotrophicus produces levan from sucrose by a secreted levansucrase (lsda). a levanase-encoding gene ( lsdb), starting 51 bp downstream of the lsda gene, was cloned from strain srt4. the lsdb gene (1605 bp) encodes a protein (calculated molecular mass 58.4 kda) containing a putative 36-amino-acid signal peptide at the n-terminus. the deduced amino acid sequence shares 34%, 33%, 32%, and 29% identities with levanases from actinomyces naeslundii, bacillus subtilis, paenibacillu ...200212029520
insights into the structure and dynamics of the dinuclear zinc beta-lactamase site from bacteroides fragilis.herein, we report quantum chemical calculations and molecular dynamics (md) simulations of the dinuclear form of the bacteroides fragilis zinc beta-lactamase. we studied four different configurations which differ in the protonation state of the asp103 residue and in the presence or absence of a zn1-oh-zn2 bridge. the flexibility of the zn1-oh-zn2 bridge was studied by means of quantum mechanical (qm) calculations on cluster models while the relative stabilities of the different configurations we ...200212022865
identification of a series of tricyclic natural products as potent broad-spectrum inhibitors of metallo-beta-lactamases.this work describes the discovery and characterization of a novel series of tricyclic natural product-derived metallo-beta-lactamase inhibitors. natural product screening of the bacillus cereus ii enzyme identified an extract from a strain of chaetomium funicola with inhibitory activity against metallo-beta-lactamases. sb236050, sb238569, and sb236049 were successfully extracted and purified from this extract. the most active of these compounds was sb238569, which possessed k(i) values of 79, 17 ...200212019104
new findings in beta-lactam and metronidazole resistant bacteroides fragilis group.beta-lactam antibiotics and 5-nitroimidazoles have been extensively used against anaerobic bacteria. however, antibiotic resistance is increasingly common among anaerobic gram-negative bacilli. the classical mechanisms of resistance to beta-lactams are, (1) production of beta-lactamases; (2) alteration of penicillin-binding proteins (pbps); and (3) changes in outer membrane permeability to beta-lactams. the 5-nitroimidazole molecule is a prodrug whose activation depends upon reduction of the nit ...200212007843
evaluation of n-acetylchitooligosaccharides as the main carbon sources for the growth of intestinal bacteria.n-acetylchitooligosaccharides ((glcnac)(n)) with different degrees of polymerization (n=1-6) were prepared as the main carbon sources in media for evaluating the growth of nine intestinal bacteria. a chitohydrolysate was prepared by hydrolyzing shrimp-shell chitin using hcl. after purification, the purity of each (glcnac)(1-6) was >86%. the growth of intestinal bacteria was carried out in a basal medium (bm) containing 0.2% (w/v) of each sugar or glucose as the main carbon source and was evaluat ...200212007653
effect of dietary inulin supplementation on inflammation of pouch mucosa in patients with an ileal pouch-anal anastomosis.inflammation is a constant finding in the ileal reservoir of patients with an ileal pouch-anal anastomosis and is associated with decreased fecal concentrations of the short chain fatty acid butyrate, increased fecal ph, changes in fecal flora, and increased concentrations of secondary bile acids. in healthy subjects, inulin, a dietary fiber, is fermented to short chain fatty acids and leads to a lower ph and potentially beneficial changes in fecal flora. the aim of the present study was to inve ...200212004211
in vitro bactericidal activity and post-antibiotic effect of abt-773 versus co-amoxiclav against anaerobes. 200212003989
the biological activity of a liposomal complete core lipopolysaccharide vaccine.a vaccine that induces humoral immunity to lipopolysaccharide (lps), while remaining non-pyrogenic should be beneficial, as high levels of antibodies against lps are associated with a reduced risk of adverse outcome. however, pure lps or bacteria expressing lps are generally considered too toxic to be used as vaccines. recently, a novel, immunogenic complete core lipopolysaccharide vaccine has been described, which has been designed to prevent endotoxin-related inflammatory reactions in surgical ...200211981444
pericarditis due to anaerobic bacteria.this review describes the microbiology, diagnosis and management of pericarditis due to anaerobic bacteria. the predominant anaerobes recovered from patients with pericarditis were: gram-negative bacilli (mostly of the bacteroides fragilis group), anaerobic streptococci, clostridium spp., fusobacterium spp., and bifidobacterium spp. anaerobic bacteria can be isolated in pericarditis resulting from the following mechanisms: (1) spread from a contiguous focus of infection, either de novo or after ...200211978949
[post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria].as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, monobactams, and carbapenems. changes in czop susceptibility for the bacteria were also evaluated with the bacterial resistance ratio calculated with the breakpoint mic. twenty-five species (3,362 strains) of gram-negative bacteria were isolated from the clinica ...200211977920
physical and genetic map of the bacteroides fragilis ych46 chromosome.the chromosome of bacteroides fragilis strain ych46 was shown to be a single circular dna molecule of about 5.3 mb having 16 noti, seven asci, and six i-ceui sites. a physical map of the chromosome was constructed by four independent experimental approaches: linking clone analysis, cross-southern hybridization, partial restriction digestion, and two-dimensional pulsed-field gel electrophoresis. six rrna operons and 10 known genes were localized on the physical map.200211958939
diversity of the metalloprotease toxin produced by enterotoxigenic bacteroides fragilis.enterotoxigenic bacteroides fragilis (etbf) strains produce a 20-kda zinc metalloprotease toxin (bft) associated with diarrheal disease of animals, young children, and adults. bft stimulates secretion in intestinal loops in vivo and modifies epithelial cell morphology in vitro. the b. fragilis toxin (bft) gene from etbf strain 86-5443-2-2 (piglet; bft-2) revealed significant nucleotide and predicted amino acid differences when compared to the bft gene from etbf strain vpi 13784 (lamb; bft-1). th ...200211953383
from bacteria to mitochondria: aconitase yields surprises. 200211929988
high prevalence of cfxa beta-lactamase in aminopenicillin-resistant prevotella strains isolated from periodontal pockets.this prospective study was designed to investigate amoxicillin-resistant oral anaerobes, and to identify their beta-lactamase-encoding genes. three subgingival bacterial samples were collected from 12 patients suffering from periodontitis. one to seven beta-lactamase-producing strains were obtained from each patient, mostly belonging to the prevotella genus (bacteroides eggerthii, 2/35 strains; prevotella sp., 33/35 strains). pcr assays were used to detect cfxa and cepa/cbla, the genes encoding ...200211929554
recent developments in carbapenems.carbapenems are beta-lactam antibiotics characterised by the presence of a beta-lactam ring with a carbon instead of sulfone in the 4-position of the thyazolidinic moiety. the first carbapenem to be utilised in therapy was imipenem, the n-formimidoyl derivative of thienamycin. imipenem is coadministered with cilastatin, an inhibitor of human renal dehydropeptidase i, as imipenem is hydrolysed by this enzyme. meropenem was the first carbapenem with a 1-beta-methyl group and 2-thio pyrrolidinyl mo ...200211922861
isolation and characterization of clv25, a bacteroides fragilis chromosomal transfer factor resembling multiple bacteroides sp. mobilizable transposons.horizontal dna transfer contributes significantly to the dissemination of antibiotic resistance genes in bacteroides fragilis. to further our understanding of dna transfer in b. fragilis, we isolated and characterized a new transfer factor, clv25. clv25 was isolated from b. fragilis lv25 by its capture on the nonmobilizable escherichia coli-bacteroides shuttle vector pgat400deltabglii. similar to other bacteroides sp. transfer factors, clv25 was mobilized in e. coli by the conjugative plasmid r7 ...200211889096
characterization of clostridium perfringens strains isolated from polish patients with suspected antibiotic-associated diarrhea.the aim of our research was to investigate the role of enterotoxin- producing anaerobic bacteria other than clostridium difficile in the etiology of antibiotic-associated diarrhea. this article presents data related to c. perfringens.200211887023
combined coliform and anaerobic infection of the lacrimal sac.a case is reported of combined coliform and anaerobic bacterial infection of the lacrimal sac, a condition of which there is only one other published case report. in addition, a literature review is presented of the bacteriology of acute dacryocystitis as it applies to this case. recommendations for the microbiological investigation and management of acute dacryocystitis are made.200211885798
[the effect of antibiotic therapy on lung pathology in experimental models of sepsis].in this study, the effect of an extra-lung sepsis model on lung histopathology is evaluated.200211881307
a mitochondrial-like aconitase in the bacterium bacteroides fragilis: implications for the evolution of the mitochondrial krebs cycle.aconitase and isocitrate dehydrogenase (idh) enzyme activities were detected in anaerobically prepared cell extracts of the obligate anaerobe bacteroides fragilis. the aconitase gene was located upstream of the genes encoding the other two components of the oxidative branch of the krebs cycle, idh and citrate synthase. mutational analysis indicates that these genes are cotranscribed. a nonpolar in-frame deletion of the acna gene that encodes the aconitase prevented growth in glucose minimal medi ...200211880608
characterization of omp200, a porin gene complex from bacteroides fragilis: omp121 and omp71, gene sequence, deduced amino acid sequences and predictions of porin structure.the high mw porin protein complex (omp200, composed of omp121 and omp71) from bacteroides fragilis atcc 25285 was purified and tryptic peptide sequences were used to design degenerate oligonucleotide primers which were then used as a first step in amplification, identification and sequencing of the omp121 gene (genbank accession number af357210). sequence analysis revealed an open reading frame of 3378 bases. the deduced amino acid sequence (which contained the experimentally determined peptide ...200211867216
evaluation of the rapid id 32a system for the identification of the bacteroides fragilis group.objective: to evaluate the use of a rapid identification system, rapid id 32a (biomérieux), for the identification of clinically important species in the b. fragilis group. methods: the use of rapid id 32a was validated on 249 clinical isolates, all of which were tested by conventional techniques, and in selected instances api 20a. rapid id 32a (and api 20a as appropriate) was then applied in a central laboratory to the identification of 1289 b. fragilis group clinical isolates from 22 laborator ...199611866828
enhancement of growth of group a beta-hemolytic streptococci in mixed infections with aerobic and anaerobic bacteria.objective: to evaluate the potential for mutual enhancement of growth of group a beta-hemolytic streptococci (gabhs), and 11 commonly associated aerobic and anaerobic microorganisms frequently isolated in tonsillar infections. methods: enhancement was assessed by measuring the relative increase in colony-forming units (cfu) of gabhs and each of the 11 microorganisms inducing a subcutaneous abscess in mice. results: of the 11 combinations of gabhs and aerobe or anaerobe, gabhs was enhanced in 10 ...199611866754
endocarditis caused by multiply resistant bacteroides fragilis: case report and review.a 78-year-old woman developed fatal endocarditis of her prosthetic aortic valve, caused by bacteroides fragilis fragilis, and associated with ovarian carcinoma. the strain showed multiple antibiotic resistance, including resistance to beta-lactam agents and combinations with beta-lactamase inhibitors. seventeen previously described cases of endocarditis caused by bacteroides spp. have been found in the literature. the mean age of the 18 patients was 50.3 years, the gastro-intestinal tract was th ...199511866720
bal 9141, a new broad-spectrum pyrrolidinone cephalosporin: activity against clinically significant anaerobes in comparison with 10 other antimicrobials.the in vitro potency of bal 9141, a new pyrrolidinone cephalosporin, was tested against non-duplicate strains of anaerobic bacteria. the mic(50) was 1 mg/l against actinomyces species, clostridium species, gram-positive anaerobic cocci, porphyromonas species, fusobacterium species, lactobacillus species, prevotella species and veillonella species. the mic(50) was 16 mg/l for bacteroides fragilis and other bacteroides species. bal 9141 was not active against cefoxitin-resistant bacteroides fragil ...200211864955
pharmacodynamic properties of hmr 3004, a novel ketolide, on respiratory pathogens, enterococci and bacteroides fragilis demonstrated by studies of time kill kinetics and postantibiotic effect.objective: the pharmacodynamic properties of the novel ketolide (a new class of macrolide) antibiotic, hmr 3004, were investigated by studying time-kill kinetics and postantibiotic effect. methods: the time-kill kinetics were studied at two inocula against three strains each of staphylococcus aureus, haemophilus influenzae, moraxella catarrhalis, streptococcus pneumoniae, streptococcus pyogenes, enterococcus faecalis, enterococcus faecium and bacteroides fragilis. the postantibiotic effects of h ...199811864324
antianaerobic activity of a cecropin---melittin peptide.objective: several small, 15-residue peptides that contain portions of the amino acid sequences of both cecropin a and melittin have previously been shown to have broad-spectrum antibacterial activities against aerobic microorganisms, with no undesirable hemolytic properties. it would also be useful to know what effect these hybrid peptides have on anaerobic bacteria. methods: the minimum inhibitory concentrations of one hybrid, ca(1--7)m(2--9)nh2, were compared with those of seven other antimic ...199811864323
activity of beta-lactam antimicrobial agents against clinical isolates of bacteroides fragilis in kuwait. 199811864283
early bacterial and inflammatory responses to antibiotic therapy in a model of polymicrobial peritonitis in rats.objective: to assess the consequences of different more or less selective treatments on the microbiological and inflammatory responses within the peritoneum. methods: the early effects of various antibiotic regimens were evaluated in a model of polymicrobial peritonitis with specifically prepared organisms. six regimens (amoxycillin plus gentamicin, pefloxacin, ornidazole, pefloxacin plus ornidazole, imipenem and imipenem plus gentamicin) were evaluated at 24 h and 3 days in a non-fatal model of ...199811864278
pcr ribotyping and arbitrarily primed pcr for the comparison of enterotoxigenic bacteroides fragilis strains from two polish university hospitals.objective: to study the clinical incidence and possible clonal relatedness of enterotoxigenic strains of bacteroides fragilis among pediatric and adult patients in two polish university hospitals. methods: fecal samples from 201 adults and 131 infants (with or without diarrhea) and vaginal samples from 100 pregnant women nursed in two polish university hospitals were analyzed with respect to carriage of enterotoxin-producing bacteroides fragilis (etbf). this putative pathogen was identified by c ...199711864083
postantibiotic effects with bacteroides fragilis determined by viable counts and co2 generation.objective: to study the postantibiotic effect (pae) for bacteroides fragilis after exposure to common anaerobic antimicrobials with two different methods, by viable counting and by measuring co2 generation in a bactec(r) blood culture system. methods: four strains of b. fragilis were exposed for 1, 2 and 4 h to cefoxitin, chloramphenicol, clindamycin, imipenem or metronidazole at concentrations from 1 to 16 x mic. the drugs were removed by dilution into bactec 7a(r) vials and growth determined w ...199711864080
reca and glna sequences separate the bacteroides fragilis population into two genetic divisions associated with the antibiotic resistance genotypes cepa and cfia.the sequences of part of the glutamine synthetase-encoding gene (glna) and of the reca-encoding gene (reca) were determined and aligned for 45 bacteroides fragilis isolates from different clinical and geographical origin. the patterns of sequence divergence of glna and reca were very similar. the sequences of a 303-bp fraction of reca showed 45 nucleotide substitutions, 40 of which allowed the separation of b. fragilis into two major divisions, which were not found when the deduced amino acid se ...200211863263
in vitro activity of gemifloxacin compared to seven other oral antimicrobial agents against aerobic and anaerobic pathogens isolated from antral sinus puncture specimens from patients with sinusitis.using a microbroth method for aerobes and agar dilution for anaerobes, we studied the comparative in vitro activity of gemifloxacin, three fluoroquinolones, two macrolides and two beta-lactams, against 207 aerobic and 162 anaerobic antral sinus puncture isolates. gemifloxacin was active at < or = 0.25 ug/ml against 198/207 [96%] aerobes and 127/162 [78%] anaerobes or 325/369 [88%] of all isolates and was the most active quinolone on a weight basis against gram-positive organisms. all haemophilus ...200211858906
evaluation of the rapid id 32a system for identification of anaerobic gram-negative bacilli, excluding the bacteroides fragilis group.objective: to evaluate the rapid id 32a system (biomérieux, marcy-l'etoile, france) for the identification of anaerobic gram-negative bacilli, excluding the bacteroides fragilis group. methods: five hundred and twenty-eight identified clinical isolates of non-b. fragilis group anaerobic gram-negative bacilli were tested in the rapid id 32a system, and identifications were compared with those obtained with conventional biochemical tests and gas-liquid chromatography. results: the rapid id 32a sys ...199911856276
an archival case of clinically significant infection in a surgical patient by enterotoxigenic bacteroides fragilis: pcr amplification of the metalloprotease enterotoxin gene from non-viable lyophilizates. 199911856233
antimicrobial susceptibility of non-bacteroides fragilis group anaerobic gram-negative bacilli in europe.objective: to investigate the in vitro activity of a range of anti-anaerobe antimicrobials against non-bacteroides fragilis group anaerobic gram-negative bacilli isolated in europe. methods: isolates from 15 laboratories in 13 countries were identified by conventional methods. the mics of 20 antibiotics were determined by an agar dilution method. results: there were 488 prevotella spp., 174 fusobacteria, 69 porphyromonas spp., 33 bacteroides spp., 28 bilophila wadsworthia and 16 campylobacter sp ...199911853565
[retrospective analysis of fusobacterium associated infections; experience at aomori prefectural hospital with 108 cases from 1995 to 1999].we experienced 108 cases of fusobacterium associated infections, including otolaryngeal, oral, pleuropulmonary, intraabdominal, skin and soft tissue infections, at aomori prefectural hospital during the 5 year-period from 1995 to 1999. a total of 433 organisms, included 113 fusobacterium spp. (80 fusobacterium nucleatum, 18 fusobaterium necrophorum, 5 fusobacterium varium, 4 fusobacterium mortiferum, 6 fusobacterium spp.), were recovered with an average of 4.0 organisms per case of the 108 cases ...200211852471
the regulatory effects of whey retentate from bifidobacteria fermented milk on the microbiota of the simulator of the human intestinal microbial ecosystem (shime).to investigate the effects of whey retentate from bifidobacteria fermented milk.200111851820
Displaying items 1401 - 1500 of 10047