Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| plasma cotinine, smoking, and lung cancer in china. | 2007 | 1971071 | |
| surface-disorganized, attenuated mutants of aeromonas salmonicida as furunculosis live vaccines. | a slow-growing, aminoglycoside-resistant mutant and a rapidly-growing pseudo-revertant were isolated from aeromonas salmonicida, the causative agent of salmonid furunculosis. these mutants continued to elicit a variety of classical virulence factors associated with a. salmonicida pathogenesis. they differed morphologically from the wild-type and from one another with respect to a-layer organization, membrane antagonist sensitivity and particularly to aerobic metabolism. both mutants were drastic ... | 1991 | 1961112 |
| self-acceptance, acceptance of others, and symlog: equivalent measures of the two central interpersonal dimensions? | after 50 hours of small group participation during 9 weeks, 91 young adults rated each same-group member's conduct on symlog's dimensions of dominance, friendliness, and task-orientedness. earlier, they made similar ratings twice, several weeks apart, on separate measures of self-acceptance and acceptance of others. individuals' mean symlog dominance ratings by group peers correlated much more highly with aggregated ratings for self-acceptance (.83) than for other-acceptance (.02), while symlog ... | 2007 | 1939704 |
| anesthesia for male aesthetic surgery. | the male patient for aesthetic plastic surgery should expect to have a good experience, with few, if any complications from anesthesia. proper planning is essential to the process, from setting up the surgical facility to selection and preparation of patients. anesthetic techniques should be adapted to the needs of each patient, with his safety and comfort the most important consideration. anesthesia for plastic surgery has progressed from simple injection of local anesthetics to sophisticated s ... | 2006 | 1934898 |
| society, health, and the nhs. | 2007 | 1932997 | |
| significance of the p210 versus p190 molecular abnormalities in adults with philadelphia chromosome-positive acute leukemia. | we investigated the significance of p210 and p190 molecular abnormalities in 32 adults with philadelphia chromosome (ph)-positive acute leukemia. p210 was detected in 15 patients (47%), p190 in 16 (50%), and both in one (3%). p210 was noted in 11 of 24 patients (46%) with acute lymphocytic leukemia, and in four of eight patients (50%) with acute myelogenous or undifferentiated leukemia. among 29 patients with untreated disease (p210, 14 patients; p190, 15 patients), no significant differences in ... | 2007 | 1932753 |
| psychological stress-induced increases in noradrenaline release in rat brain regions are attenuated by diazepam, but not by morphine. | by measuring levels of noradrenaline (na) and its major metabolite, 3-methoxy-4-hydroxyphenylethyleneglycol sulfate (mhpg-so4) in the hypothalamus, amygdala and locus coeruleus region, we investigated the effects of diazepam 5.0 mg/kg, morphine 6.0 mg/kg, or naloxone at 5.0 or 10 mg/kg injected sc immediately before stress exposure, on increases in na release caused by psychological stress. psychological stress, wherein rats were exposed to emotional responses which were displayed by other elect ... | 1991 | 1924502 |
| dynamic computed tomography as an ancillary screening test for traumatic thoracic aortic injuries: a critical review. | while the divergence of opinion on the matter of diagnostic modalities will probably continue, dynamic computed tomography scans should be considered as a necessary study in assessing a possible traumatic thoracic aortic injury. the use of this modality will decrease the need for thoracic angiograms and invariably increase the yield. | 2007 | 1920521 |
| atypical characteristics of the salmonid pathogen aeromonas salmonicida. | incubation of aeromonas salmonicida at supra-optimal temperatures, i.e. 30-37 degrees c, resulted in the expression of motility by polar flagella, and changes in sugar fermentation patterns, e.g. loss of acid production from mannitol, loss of the ability to degrade complex molecules (aesculin, dna, elastin and gelatin), and an increase in antibiotic resistance (notably co-trimoxazole). motility was enhanced in cultures grown in brain heart infusion broth supplemented with 18% (w/v) ficoll. | 1991 | 1919508 |
| [a statistical model of evolution with stabilizing selection]. | we consider a population of fixed size and reproducing asexually, evolving in a rugged fitness landscape. selection takes place only via the elimination of individuals with unfit genomes. unfit genotypes are distributed at random in genotypic space. the genetic structure of the population and the speed of genetic drift are explicitly computed in the infinite genome limit. | 2007 | 1913251 |
| effect of diabetes and aging on carboxymethyllysine levels in human urine. | carboxymethyllysine (cml) has been identified as a modified amino acid that accumulates with age in human lens proteins and collagen. cml may be formed by oxidation of fructoselysine (fl), the amadori adduct formed on nonenzymatic glycosylation of lysine residues in protein, or by reaction of ascorbate with protein under autoxidizing conditions. we proposed that measurements of tissue and urinary cml may be useful as indices of oxidative stress or damage to proteins in vivo. to determine the ext ... | 1991 | 1899406 |
| introduction of vascular smooth muscle cells expressing recombinant genes in vivo. | vascular smooth muscle cells contribute to the formation of atherosclerotic plaques by proliferating in response to vascular injury and releasing growth-promoting factors. because their autocrine and paracrine effects are not fully understood, expression of such growth factor genes in specific cell types in vivo would help to determine their mechanism of action. we describe a method to transfer vascular smooth muscle cells expressing recombinant gene products to localized segments of the arteria ... | 1991 | 1899366 |
| does prestretching stainless-steel wire have any clinical significance? | clinical parameters of prestretch and tension forces placed on stainless-steel wire by 20 oral and maxillofacial surgeons were obtained. unstretched wire was clinically as tough or tougher than prestretched wire of a similar gauge. both prestretched and unstretched wires, twisted to their maximum, loosened when under tension. as the prestretch forces increased, the clinical strength of the wire diminished. | 2007 | 1890520 |
| health status of salmonids in river systems in natal. iii. isolation and identification of bacteria. | both pathogenic and non-pathogenic bacteria were isolated from fish, both salmonid and non-salmonid, from selected river systems in natal. pasteurella pisicida was isolated for the first time from fish in south africa. the isolation of yersinia ruckeri, aeromonas salmonicida, and edwardsiella tarda were recorded for the first time from fish in natal. a. hydrophila and flexibacter columnaris were found to be widespread throughout the river systems in natal. the streptococcus species which caused ... | 1991 | 1881658 |
| survival of aeromonas salmonicida in lake water. | the survival of aeromonas salmonicida subsp. salmonicida in lake water was investigated by using a variety of techniques. they included acridine orange epifluorescence, respiration, cell culture, cell revival, flow cytometry, plasmid maintenance, and membrane fatty acid analysis. during a 21-day study, a. salmonicida became nonculturable in sterile lake water samples. flow cytometry and direct microscopy indicated that cells were present. although the nonculturable cells could not be revived, th ... | 1991 | 1872606 |
| structure of the tetragonal surface virulence array protein and gene of aeromonas salmonicida. | the paracrystalline surface protein array of the pathogenic bacterium aeromonas salmonicida is a primary virulence factor with novel binding capabilities. the species-specific structural gene (vapa) for this array protein (a-protein) was cloned into lambda gt11 but was unstable when expressed in escherichia coli, undergoing an 816-base pair deletion due to a 21-base pair direct repeat within the gene. however, the gene was stable in cosmid pla2917 as long as expression was poor. a-protein was lo ... | 1991 | 1869553 |
| a citation analysis of the work of lee b. lusted, md. | 2007 | 1865779 | |
| human serum stimulates endothelin-1 synthesis more potently than prostacyclin production by cultured vascular endothelial cells. | human serum stimulated the synthesis of a vasoconstrictive peptide, endothelin-1 (et-1), and a vasodilatory prostanoid, prostacyclin (pgi2), by cultured human umbilical vein endothelial cells in a concentration- and time-dependent manner. incubation in 20% concentration of the serum for 24 h stimulated et-1 synthesis almost six-fold while pgi2 production increased two-fold. in addition, a tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate (pma), inhibited the serum-induced et-1 produ ... | 1991 | 1865754 |
| site-directed mutagenesis of a single tryptophan near the middle of the channel-forming toxin aerolysin inhibits its transfer across the outer membrane of aeromonas salmonicida. | the channel-forming protein aerolysin must cross both the inner and outer bacterial membranes during its secretion from aeromonas hydrophila or from aeromonas salmonicida containing the cloned structural gene. we examined the fate of three mutant proteins in which trp-227, near the middle of the amino acid chain, was replaced with glycine, leucine, or phenylalanine by site-directed mutagenesis. all three proteins crossed the inner membrane and entered the periplasm in the same way as wild-type, ... | 1991 | 1860853 |
| analysis and pyrolysis of some n-nitrosamino acids in tobacco and tobacco smoke. | a new tobacco-specific nitrosamine, 4-(n-nitrosomethylamino)-4-(3-pyridyl)butyric acid (iso-nnac), has been identified in tobacco, and its structure was confirmed by gas chromatography-mass spectrometry following enrichment of a tobacco extract. the levels of iso-nnac ranged from 0.01 to 0.95 ppm. it does not induce dna repair in primary rat hepatocytes and is inactive as a tumorigenic agent in strain a mice. in order to study the fate of nitrosamino acids during smoking, we spiked cigarettes wi ... | 1991 | 1855899 |
| high blood pressure during pregnancy and working conditions among hospital personnel. | the relationship between working conditions and high blood pressure during pregnancy was analysed in a sample of 621 women hospital employees in the paris region over the period 1979-1981. data were collected by interviews during the routine medical visit at the end of postnatal leave. women who had to work standing up for extended periods of time, who had to carry heavy loads or who had to perform heavy cleaning tasks had high blood pressure during their pregnancy more often than women not expo ... | 1991 | 1855606 |
| [assisted ventilation: prevention of nosocomial infections]. | 2007 | 1853087 | |
| effect of monocular enucleation or impulse blockage on gamma-aminobutyric acid and cytochrome oxidase levels in neurons of the adult cat lateral geniculate nucleus. | much attention has been paid to the effect of various types of neonatally induced retinal insults on neurons of the cat lateral geniculate nucleus (lgn). little is known about cellular adjustment to functional alterations commencing in the adult. the present study was aimed at examining the effect of monocular enucleation or retinal impulse blockade on mature neurons of the cat lgn and perigeniculate nucleus. in addition to labeling the relay neurons with cytochrome oxidase (co) histochemistry a ... | 1991 | 1851037 |
| isolation and phenotypic characterization of an oxidase-negative aeromonas salmonicida causing furunculosis in coho salmon (oncorhynchus kisutch). | an oxidase-negative aeromonas salmonicida was isolated from coho salmon (oncorhynchus) kisutch) suffering from an epizootic of furunculosis at the state hatchery near belfair, washington. typical, oxidase-positive a. salmonicida was isolated concurrently from the same population of fish. mortality was controlled with medicated feed treatments. evidence supporting the identification of the two types of a. salmonicida is presented. methods for the proper identification of oxidase-negative a. salmo ... | 1991 | 1850808 |
| siderophore production by aeromonas salmonicida. | growth under conditions of iron-restriction and the production of siderophores was examined in 21 typical and 14 atypical strains of aeromonas salmonicida. with the exception of one atypical strain, all strains grew and multiplied in the presence of the high-affinity iron chelators ethylenediamine di(o-hydroxyphenylacetic acid), alpha, alpha'-dipyridyl or transferrin. chrome azurol s agar was used to screen bacterial strains growing under these conditions for the production of siderophores. side ... | 1991 | 1830896 |
| the arcuate line of the rectus sheath--does it exist? | the lower halves of the anterior abdominal wall of 40 human specimens of both sexes and various ages were studied. the anatomical features seen were widely variable and quite different from the conventional picture. the fibres of the posterior lamina of the internal oblique and transversus abdominis aponeuroses gradually shift from the posterior to the anterior rectus sheath starting from the level of the umbilicus down to the level of the symphysis pubis. thus, these anterior laminae gradually ... | 1991 | 1828798 |
| production of colony-stimulating factors during pneumonia caused by chlamydia trachomatis. | the colony-stimulating factors (csfs) are cytokines involved in the production, differentiation, and activation of host phagocytes. during murine infection with chlamydia trachomatis (mopn), plasma csf levels increased in euthymic (nu/+) and athymic (nu/nu) balb/c mice. levels declined later in infection, with the nu/+ mice resolving the infection but the nu/nu mice succumbing by day 16. either live or heat-killed chlamydia organisms could induce csf increases on day 7 postchallenge in nu/+ mice ... | 1991 | 1828791 |
| in vitro susceptibility of the fish pathogen aeromonas salmonicida to flumequine. | the activity of the fluoroquinolone flumequine was investigated against the fish pathogen aeromonas salmonicida and was compared with that of oxolinic acid. flumequine was more active than oxolinic acid in terms of its mic against oxolinic acid-resistant isolates of a. salmonicida and was as active as oxolinic acid against susceptible isolates. in contrast to oxolinic acid, flumequine was bactericidal, with only 1% of the bacteria surviving 6 h of exposure to the drug at concentrations slightly ... | 1991 | 1810198 |
| cyanide antagonism in a rodent model. | 1991 | 1805737 | |
| survival of the fish pathogen aeromonas salmonicida in seawater. | survival of aeromonas salmonicida in natural (non-sterile) seawater, as determined from colony counts on marine agar, was found to be influenced by the presence of potentially inhibitory organisms, i.e., acinetobacter, aeromonas hydrophila, chromobacterium, escherichia coli, flavobacterium and pseudomonas, and their metabolites. yet, samples, thought to be devoid of culturable a. salmonicida, were found to contain cells, which were filterable through 0.22 and 0.45 microns millipore millex porosi ... | 1991 | 1769545 |
| isolation and characterization of turbot (scophtalmus maximus)-associated bacteria with inhibitory effects against vibrio anguillarum. | more than 400 isolates from the intestine and the external surface of farmed scophtalmus maximus as well as from fish food and hatchery water were screened for inhibitory effects against the fish pathogen vibrio anguillarum hi 11345 and seven other fish pathogens. the bacteria with inhibitory effects were then characterized with regard to their sites of colonization, especially the intestinal regions and sites within each region. of the total number of bacterial isolates from the intestine, 28% ... | 1991 | 1768092 |
| binding of laminin and fibronectin by the trypsin-resistant major structural domain of the crystalline virulence surface array protein of aeromonas salmonicida. | the surface of aeromonas salmonicida is covered by a tetragonal paracrystalline array (a-layer) composed of a single protein (a-protein, mr = 50,778). this array is a virulence factor. cells containing a-layer and isolated a-layer sheets specifically bound laminin and fibronectin with high affinity. binding by cells was inactivated by selective removal of a-layer at ph 2.2, and neither isogenic a-layer-deficient a. salmonicida mutants nor tetragonal paracrystalline array producing aeromonas hydr ... | 1992 | 1730607 |
| developmental cycle-specific host-free rna synthesis in chlamydia spp. | the incorporation of radiolabeled gtp into rna in host-free chlamydia trachomatis serovar l2 organisms was investigated. the incorporation was partially inhibited by rifampin and dactinomycin and hydrolyzed by rnase. rna made by host-free chlamydiae consisted mainly of species of fewer than 800 bases in size, although 16s and 23s species were noted by agarose-gel electrophoresis. the hybridization of radiolabeled host-free rna to restriction fragments of the gene encoding the major outer membran ... | 1990 | 1698176 |
| preliminary results of intensive therapy of children and adolescents with acute nonlymphocytic leukemia--a childrens cancer study group report. | 2002 | 1691130 | |
| evaluation of the child with idiopathic mental retardation. | a review of the advances in diagnostic techniques for evaluation of children with idiopathic mental retardation is presented. the current status of the use of clinical genetics, cytogenetics, molecular genetics, and neuroimaging in evaluating children with mental retardation is emphasized. special attention is given to the evaluation of children with mental retardation and "autism" or "cerebral palsy." | 2006 | 1635812 |
| toxicity of aeromonas salmonicida cells to atlantic salmon salmo salar peritoneal macrophages. | several strains of aeromonas salmonicida were toxic to cultured peritoneal macrophages of atlantic salmon, salmo salar, in minimum doses of 5 x 10(6) cfu, whereas other strains were not. there was no correlation between cytotoxicity and in vivo virulence of the bacteria, the presence or absence of the two major surface components of a. salmonicida (namely, the a-layer and the o-polysaccharide chain of the lps) nor, finally, with the ability of the strains to produce the following enzymes in vitr ... | 1992 | 1618355 |
| glycosidase activities in gingival crevicular fluid in subjects with adult periodontitis or gingivitis. | specific glycosidase activities were determined in samples of gingival crevicular fluid (gcf) collected from eight predetermined sites in two groups, each of 20 adult patients, with either gingivitis or periodontitis. the total activities (as units of enzyme activity per sample) of alpha-l-fucosidase, sialidase, beta-n-acetylglucosaminidase, beta-galactosidase, beta-glucosidase and alpha-glucosidase were significantly greater in the periodontitis group. in contrast, the total beta-mannosidase an ... | 2006 | 1610303 |
| a comparison of total and specific immunoglobulin levels in healthy atlantic salmon (salmo salar l.) and in salmon naturally infected with aeromonas salmonicida subsp. achromogenes. | healthy atlantic salmon and salmon with a history of chronic natural aeromonas salmonicida subsp. achromogenes infection were compared with respect to total serum protein and the concentration and specificity of serum immunoglobulin. the immunoglobulin level was measured using competitive elisa and the specific antibody activity against aeromonas salmonicida subsp. achromogenes was measured using double sandwich elisa. significant elevation of serum protein and immunoglobulin concentration was o ... | 1992 | 1604799 |
| [mono-arthritis of uncertain etiology--a follow-up study]. | 78 patients in whom the cause of a monoarticular arthritis remained unclear after an initial workup were contacted for a follow-up interview (and an additional clinical examination in 64 cases) after 6 to 11 years (mean 8 years). the mean age at the onset of symptoms was 39 years, with 51% of the patients presenting in the range between 20 and 40 years. there was a slight male preponderance (56%). large joints, mainly the knee, and less often the wrist, ankle or hip, were affected in 79%. finger ... | 1992 | 1594908 |
| role of catecholamines in the courtship behavior of male ring doves. | the role of catecholamines in the expression of male courtship behavior in ring doves was examined using central administration of pharmacological agents. males treated with 6-hydroxydopamine or u-14,624, which depleted norepinephrine (ne) levels in the preoptic-hypothalamic area, showed increased levels of bow-coo and nest-coo displays. conversely, males treated with tyramine or desipramine, which elevated ne levels in the preoptic-hypothalamic area, showed decreased levels of bow-coo and nest- ... | 2006 | 1594642 |
| [the treatment concept and results of peri-/sub-prosthetic fractures]. | the treatment of choice in ipsilateral femoral shaft fractures in patients with hip prosthesis depends on the location of the fracture lines and the observation of loosening of the prosthesis. either a change of prosthesis, if necessary in combination with refixation of the greater trochanter or simple open reduction with internal fixation (asif) along with autologous and/or homologus spongiosa are the used techniques. the rate of complications in peri- or subprothetic fractures is high. lack of ... | 2006 | 1590026 |
| dna probe for aeromonas salmonicida. | a dna fragment that is specific to aeromonas salmonicida has been isolated from a genomic dna library by differential hybridization. the specificity of this fragment as a dna probe for a. salmonicida was shown by hybridization against reference strains and clinical isolates of a. salmonicida, related aeromonads, and species from several other bacterial genera. the sensitivity of detection by a polymerase chain reaction test, based on this fragment, was approximately two a. salmonicida cells. | 1992 | 1575477 |
| pulsatile growth hormone secretion during the breeding season in male reindeer and its association with hypophagia and weight loss. | male deer reduce food intake and lose weight during the breeding season (rut) in autumn. growth hormone (gh) is lipolytic in ruminants; therefore, pulsatile gh secretion was measured in three young reindeer (rangifer tarandus tarandus) bulls during the natural period of weight loss during rut. blood samples were withdrawn through a jugular cannula every 20 min for 16 hr on 5 occasions from late summer to early winter in unanaesthetised animals. the deer were group fed a pelleted concentrate rati ... | 1992 | 1563616 |
| animal rights/liberation philosophy. | 2006 | 1563603 | |
| reform and restructuring in nursing education: a necessity for nursing's future in florida. | 2006 | 1563527 | |
| executive empowerment enhances profession. | 2006 | 1560269 | |
| nurse prescribing: how and when. | legislation for nurse prescribing now seems destined to be implemented in october 1993, writes judith moreton who is on the nurse prescribers formulary subcommittee. a private members bill from chislehurst mp roger sims, received its second reading on the 31 january. it has full government support and should become law in this session of parliament. | 2005 | 1559831 |
| amoxycillin in the control of furunculosis in atlantic salmon parr. | the efficacy of amoxycillin in the control of laboratory induced aeromonas salmonicida infection in atlantic salmon parr was investigated. when given in the diet at a dose rate of 80 mg per kg bodyweight it was effective against both a moderate and severe challenge (with mortality rates in untreated groups of 75 per cent and 45 per cent). at 40 mg per kg it was effective against the moderate challenge only. the plasma levels in these regimens were 1.25 micrograms per ml and 0.3 to 0.6 micrograms ... | 1992 | 1546434 |
| effect of monensin and supplemental hay on ruminal 3-methylindole formation in adult cows after abrupt change to lush pasture. | the effect of feeding monensin, with or without dry hay plus wilted forage, on ruminal formation of 3-methylindole (3mi) was investigated in pastured cattle. eighty-two cows were allotted to 3 groups. cows of group-1 served as controls and were given a daily energy supplement (1 kg/head) without monensin for 1 day before and for 7 days after being allowed access to lush pasture. cows of groups 2 and 3 were given the same daily energy supplement, which also contained monensin (200 mg/kg of supple ... | 1992 | 1539904 |
| relationship between intracellular ph and tension development in resting ventricular muscle and myocytes. | simultaneous measurements of intracellular ph (phi) and tension development were made in resting cat papillary muscles and resting ventricular myocytes (cat, guinea pig). ph microelectrodes and the fluorescent indicator carboxy-seminaphthorhodafluor-1 (snarf-1) were used to measure phi in muscles and myocytes, respectively. phi-induced changes in isometric muscle tension and myocyte length were elicited by variations in pco2, hco3-, and [nh4cl]. increased phi elevated resting tension and decreas ... | 1992 | 1539624 |
| occurrence of a capsule in aeromonas salmonicida. | aeromonas salmonicida grown in a medium with excess glucose as carbon source produces both capsular and exocellular polysaccharides. the capsular polysaccharide is composed of glucose, mannose, rhamnose, n-acetylmannosamine and mannuronic acid in the molar ratios of approximately 5:3:0.75:2:1. the extracellular polysaccharide is similarly constituted, but in the molar ratios of approximately 4.75:10.5:1.5:2:1. the capsular and exocellular polysaccharides did not cross-react with monoclonal antib ... | 1992 | 1526444 |
| erythropoietin use in the critical care setting. | biotechnology products signify a major advancement in our world today. products resulting from biotechnology will revolutionize how health care is delivered. one of these technologic breakthroughs is recombinant human erythropoietin (epoetin). its impact on the delivery of care to the anemic renal patient is changing the roles of nurses who provide care for these patients. epoetin alfa has virtually eliminated the necessity of transfusions in the renal patient population, while simultaneously im ... | 1992 | 1524935 |
| colotomy with minimum resection for advanced irreducible intussusception. | 2006 | 1501020 | |
| mechanisms of ion and acid-base regulation at the gills of freshwater fish. | this review examines the branchial mechanisms utilized by freshwater fish to regulate internal acid-base status and presents a model to explain the underlying basis of the compensatory processes. rainbow trout, oncorhynchus mykiss, and brown bullhead, ictalurus nebulosus, were examined under a variety of experimental treatments which induced respiratory and metabolic acid-base disturbances. acid-base regulation was achieved by appropriate adjustments of na+ and cl- net fluxes across the gills wh ... | 2006 | 1500882 |
| pay review body: body blow or a minor injury? | 2006 | 1489700 | |
| avoidance of endotoxin-induced inflammation during studies of albumin clearance from caprine joints. | intra-articular injection of radiolabelled, commercial goat serum albumin (gsa) produces acute arthritis in caprine joints. this inflammation distorts clearance values and vitiates studies of normal lymphatic function. endotoxin, routinely found in commercial albumin preparations, appears to cause this local reaction. we describe a simple method for the preparation of low-endotoxin, radioiodinated serum albumin from aseptically collected serum. we have used this technique to prepare gsa for use ... | 1992 | 1489542 |
| factors affecting the strength of flexor tendon repair. | the effects of different thicknesses and configurations of core sutures were studied in human cadaveric flexor tendon repairs. both straight and cyclic load tests were employed. to exploit the full strength of 4/0 suture material, the kessler repair using four locked single knots would seem to be appropriate. | 1992 | 1479249 |
| detection of aeromonas salmonicida from fish by using polymerase chain reaction amplification of the virulence surface array protein gene. | a dna-based assay was developed to detect aeromonas salmonicida from infected fish by analyzing tissues, feces, and the tank water in which the infected fish were held. this analysis was done both by direct detection from samples and after a bacterial outgrowth step. polymerase chain reaction (pcr) amplification of a 421-bp sequence from the 3' region of the surface array protein gene (vapa) of a. salmonicida provided a specific and sensitive method for the detection and identification of this i ... | 1992 | 1476426 |
| asterric acid, a new endothelin binding inhibitor. | 1992 | 1473998 | |
| the cloning and nucleotide sequence of the serine protease gene (aspa) of aeromonas salmonicida ssp. salmonicida. | the gene for aeromonas salmonicida serine protease has been cloned into phagemid ptz18r in two restriction fragments, 2.0-kb psti and 2.3-kb kpni, of genomic dna. the nucleotide sequences of the two fragments have been determined, in both directions, after subcloning, by double-stranded sequencing of nested deletions. an open reading frame of 1863 bp translated into a sequence of 621 amino acids, a 24-amino acid signal peptide and a 597-amino acid mature enzyme of molecular mass 64,173 da. the c ... | 1992 | 1468618 |
| identification of a cyprinid fish, the tench tinca tinca l., as a carrier of the bacterium aeromonas salmonicida, causative agent of furunculosis in salmonids. | a typical, pigment-producing strain of aeromonas salmonicida (a. sal.), the causative agent of furunculosis in salmonid fish species, was isolated from a cyprinid species, the tench tinca tinca l. with papilloma-like skin alterations. histopathology of the papilloma-like skin alterations in tench revealed "round holes", distinctly lined by thick layers of epithelial cells, but no bacteria. the organism was isolated from skin, gills and fins, but not internal organs. the isolate proved highly vir ... | 1992 | 1462724 |
| fine needle aspiration of pancreatic cystic epithelial neoplasms. | pancreatic cystic epithelial neoplasms present diagnostic challenges in cytology. an accurate diagnosis is important since the prognosis and treatment may vary. we report the cytologic features in fine needle aspirates of four cases of cystic neoplasms of the pancreas (two micro-cystic adenomas, one mucinous cystic neoplasm and one mucinous cystadenocarcinoma). smears were evaluated as to their cellularity, content and predominant cell type. aspirates from the microcystic adenomas yielded hypoce ... | 2006 | 1449026 |
| modification of the disc assay by the incorporation of monoclonal antibody staining. | the use of immunohistochemical staining with the disc assay is described. this modified staining procedure allows differentiation of viable human tumor cells from normal cells in biopsy preparations, facilitating the evaluation of slides from chemosensitivity assays. samples from all and cll patients were processed using both the disc and modified moab-disc procedure. the predictive results were compared and found to be identical. this procedure is advantageous in determining the chemosensitivit ... | 1992 | 1434746 |
| phototransduction in drosophila: a paradigm for the genetic dissection of sensory transduction cascades. | a combination of molecular, genetic and physiological studies is providing fundamental insight into the function and regulation of the phototransduction cascade. the availability of drosophila mutants with defects in visual physiology allows for an in vivo dissection of this complex sensory signal transduction process. | 1993 | 1422119 |
| temperature-dependent in vitro antimicrobial activity of four 4-quinolones and oxytetracycline against bacteria pathogenic to fish. | the in vitro antimicrobial activities of oxolinic acid, flumequine, sarafloxacin, enrofloxacin, and oxytetracycline against strains of bacteria pathogenic to fish (aeromonas salmonicida subsp. salmonicida, atypical a. salmonicida, vibrio salmonicida, vibrio anguillarum, and yersinia ruckeri) were determined at two different incubation temperatures, 4 and 15 degrees c, by a drug microdilution method. the main objective of the study was to examine the effect of incubation temperature on the in vit ... | 1992 | 1416857 |
| interaction of the fish pathogen aeromonas salmonicida with rainbow trout macrophages. | a procedure was developed to culture rainbow trout macrophages (m phi) on supported glass coverslips. using this method and a variety of well-characterized aeromonas salmonicida strains with normal or altered cell surfaces, we investigated the role of this unusual bacterial surface in the bacterium-m phi interaction. an intact crystalline protein array, the a-layer, mediated adherence of a. salmonicida cells to m phi even in the absence of opsonins. in contrast, unopsonized cells of an a-layer-n ... | 1992 | 1398975 |
| s-layer-mediated association of aeromonas salmonicida with murine macrophages. | the interaction of aeromonas salmonicida with the murine macrophage (m phi) cell line p388d1 was used as a convenient model to study the involvement of the bacterial crystalline surface array (or a-layer) in the association with m phi s. a-layer-positive (a+) cells readily associated with m phi s in phosphate-buffered saline, whereas a- mutants were unable to do so, even when the bacterium-m phi interaction was forced by centrifugation. m phi s selectively interacted with a+ cells when challenge ... | 1992 | 1398947 |
| structure of the core oligosaccharide in the lipopolysaccharide isolated from aeromonas salmonicida ssp. salmonicida. | the core oligosaccharide isolated from the lipopolysaccharide of aeromonas salmonicida ssp. salmonicida has been investigated by methylation analysis, nmr spectroscopy (13c and 1h), oxidation with periodate and chromium trioxide, and smith degradation. the following structure is proposed: [formula: see text] | 1992 | 1394331 |
| health checks on patients 75 years and over in nottinghamshire after the new gp contract. | to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners. | 2006 | 1393076 |
| time vs. distance as measures of exposure in driving surveys. | a survey of drivers carried out in ontario in 1988 has provided data on time spent driving as well as the distances driven for licensed drivers of both sexes in six age groups and three regions. substantial differences were found in times, distances, and distance/time ratios among these groups. men drove 50% greater distances, but spent only 30% more time driving than women; speed, averaged over each day's driving, was lower for older drivers than for younger drivers. differences in speed reflec ... | 1995 | 1388587 |
| cardiac function in obstructive sleep apnea patients following uvulopalatopharyngoplasty. | obstructive sleep apnea syndrome (osas) is associated with severe cardiac arrhythmias and conduction abnormalities. cor pulmonale and right-sided heart failure may ensue. uvulopalatopharyngoplasty (uppp) is one of several treatment modalities suggested for osas. tracheotomy and cpap treatment in adult osas patients and adenotonsillectomy in children with osas were shown to lead to improvement in some cardiac parameters. cardiac function was prospectively evaluated in 19 osas patients before and ... | 1995 | 1383911 |
| immunochemical analysis and possible biological role of an aeromonas hydrophila surface array protein in septicaemia. | the biochemical, immunological, and biological properties of an s layer purified from an aeromonas hydrophila strain (ah-342) involved in a case of bacteraemia were investigated. the s layer selectively removed from the cell surface was composed of a single acidic (pi 4.56) protein subunit (surface array protein, sap) with a molecular mass of approximately 52 kda. amino acid analysis of this 52 kda protein indicated a molecule composed of 498 amino acids with 46% hydrophobic residues. no cystein ... | 1992 | 1382113 |
| a general method to generate dna probes for microorganisms. | we present a method that permits the rapid generation of dna probes for eubacteria. in the procedure the variable regions for the 16s rrna genes are amplified using polymerase chain reaction (pcr) technology and primers based on the conserved regions of these genes. following sequencing of the variable regions, a choice is possible for a probe specific for that organism. no knowledge of the molecular biology of the microorganism is required prior to the application of this approach. the generali ... | 1990 | 1369980 |
| chemical permeabilization of cells for intracellular product release. | 2006 | 1366880 | |
| [lipid and mineral metabolism and morbidity with temporary loss of work capacity in miners]. | examination is performed on 87 miners from mine for copper production subject to intensive local vibrations with average rate of increase (k) of the norms for the individual octave frequencies 2.4 times for 3 h exposure of the working shift, as well as to intensive noise--113-115 db/a equivalent level, general dust--23 mg/m3, fine dust--3.5 mg/m3 and fine quartz--0.43 mg/m3. there are data for vibration effect--changes in the cold test, vibration sensoriness, ultra sound sonometry, in all miners ... | 2005 | 1364539 |
| urinary kallikrein excretion in non-insulin-dependent diabetes mellitus. | to investigate the status of urinary kallikrein excretion (uke) in patients with non-insulin-dependent diabetes mellitus (niddm), we measured uke in 31 niddm patients. they ranged in age from 40 to 70 years (mean, 54.3 +/- 7.8 years), comprising 18 males and 13 females. their creatinine clearance (ccr) was 91.6 +/- 5.5 ml/min, and the daily excretion rate of protein was 1.15 +/- 0.72 g/24 hours. twenty-five normal persons, aged from 37 to 63 years (mean, 51.7 +/- 8.2 years), comprising 14 males ... | 2006 | 1360304 |
| fimbriation, capsulation, and iron-scavenging systems of klebsiella strains associated with human urinary tract infection. | thirty-two strains of klebsiella pneumoniae and seven strains of klebsiella oxytoca isolated from urinary tract infections in elderly adults were analyzed for capsular antigens, iron-scavenging systems, and fimbriation. all strains were capsulated. twenty-seven different k antigens were identified among the strains, with no particular antigen dominating. all strains produced the iron-scavenging system enterochelin as analyzed by bioassay and dna hybridization. in contrast, the aerobactin iron-se ... | 1992 | 1347287 |
| role of gastric acid suppression in the treatment of gastro-oesophageal reflux disease. | gastro-oesophageal reflux disease is a common condition with a complex pathophysiology. despite the spectrum of abnormalities, gastric acid has a central role in mucosal damage, and the mainstay of medical treatment is suppression of gastric acid secretion. the results of antisecretory treatment as assessed by endoscopic healing are reviewed. h2 receptor antagonists give more rapid symptom relief than placebo and can produce endoscopic improvement in 31-88% of cases depending on the severity of ... | 2006 | 1346769 |
| non-culturable aeromonas salmonicida in lake water. | the survival of aeromonas salmonicida subsp. salmonicida was investigated in lake water. during a 21-day study a. salmonicida became non-culturable in sterile lake water held at 10 degrees c. the incubation of replicate samples between 5 degrees c and 25 degrees c produced similar results. the recovery of colony-forming units of a. salmonicida from different lake water systems indicated that they survived longer in water that was naturally enriched (eutrophic) or enriched with tryptone soya brot ... | 1992 | 1342635 |
| glucose repression of pigment production in atypical isolates of aeromonas salmonicida responsible for goldfish ulcer disease. | the effect of glucose and other carbohydrates on the pigmentation of three atypical isolates of aeromonas salmonicida indicated that the addition of d-glucose at a concentration of 0.1% (w/v) or more, inhibited pigment production and caused a reduction in the size of colonies. the addition of camp reversed the inhibitory effects of d-glucose on pigment production. | 1992 | 1336808 |
| molecular and genetic characterization of cytochrome oxidase-negative aeromonas salmonicida isolated from coho salmon (oncorhynchus kisutch). | cytochrome oxidase variants of the bacterial fish pathogen, aeromonas salmonicida, were characterized for genetic and molecular homology with cytochrome oxidase-positive isolates that typically induce furunculosis in salmonids. protein and lipopolysaccharide moieties of the cytochrome oxidase-negative variants were similar to their typical counterparts, based on sodium-dodecyl-sulfate polyacrylamide gel electrophoresis. pathogenicity of aberrant isolates to brook trout (salvelinus fontinalis) wa ... | 1992 | 1335520 |
| inhibition of lipopolysaccharide synthesis in agrobacterium tumefaciens and aeromonas salmonicida. | lipopolysaccharide (lps) synthesis was inhibited, new lipid a metabolites accumulated, and growth ceased, when the plant pathogen agrobacterium tumefaciens and the fish pathogen aeromonas salmonicida were treated with an antibacterial agent which specifically inhibits ctp:cmp-3-deoxy-manno-octulosonate cytidylyltransferase (cmp-kdo synthase). the new lipid a metabolites were purified by chromatography on deae-cellulose and chemically analysed. metabolites isolated from both bacterial species con ... | 1992 | 1324975 |
| novel structural patterns in divalent cation-depleted surface layers of aeromonas salmonicida. | the fish pathogen aeromonas salmonicida possesses a regular surface layer (or a-layer) which is an important virulence determinant. the a-protein, a single bilobed protein organized in a p4 lattice of m4c4 arrangement with two morphological domains, comprises this layer. the role of divalent cations in the a-layer structure was studied to better understand a-protein subunit interactions affecting structural flexibility and function. divalent cation bridges were found to be involved in the integr ... | 1992 | 1296752 |
| bond strength of glass-ionomer cement and composite resin combinations. | the tensile bond strength to dentin was measured for three glass-ionomer cement and composite resin combinations: two light-curing glass-ionomer cements (vitrebond and xr-ionomer) and one traditional glass-ionomer cement (ketac-bond), two adhesive systems (scotchbond, and xr-bonding system), and a corresponding composite resin. the bond strength of this "sandwich" was also compared with that of the same cements used in bulk. vitrebond showed a significantly higher bond strength in bulk than did ... | 2005 | 1287716 |
| [ginkgo biloba--analysis and dosage forms]. | 2005 | 1287670 | |
| estimating trauma centre workload. | 2005 | 1282567 | |
| immunostimulants added to injected aeromonas salmonicida bacterin enhance the defense mechanisms and protection in rainbow trout (oncorhynchus mykiss). | immunostimulants were given to rainbow trout for assaying effects on modulating non-specific defense mechanisms, specific immune response, and protection levels against pathogen challenge with aeromonas salmonicida. three drugs, levamisole (an approved veterinary drug in the usa), a quaternary ammonium compound (qac), and a short-chain polypeptide (isk) were found to affect the non-specific defense mechanism activities, which were measured by changes in circulatory neutrophil and phagocytic acti ... | 1992 | 1280879 |
| blood changes in brook trout induced by infection with aeromonas salmonicida. | furunculosis was induced in brook trout, salvelinus fontinalis, by experimental inoculation with aeromonas salmonicida. total protein, hemoglobin, sialic acid, fatty acids, triglycerides, cholesterol, inorganic-phosphorus, acid-soluble phosphorus, and lipid-phosphorus decreased in the blood of the infected fish while amino acids, urea, total creatinine, ammonia, and glucose increased. pyruvic acid, lactic acid, and ascorbic acid values showed no significant change. | 1976 | 1255917 |
| genetic influence on saline consumption and salt hypertension as exhibited by the response of various rat strains and sub-strains. | female rats of 2 sprague-dawley sub-strains and of the wistar and fischer 344 strains were sensitized to the hypertensogenic effect of excess salt by removal of 1 kidney and by being given 1% nacl solution as their sole drinking fluid. one of the sprague-dawley sub-strains (spd) and the wistar (cfep) rats developed progressive saline polydipsia of equivalent degree, blood pressure rose, and about half of the members became hypertensive during treatment. rats of the other sprague-dawley strain (c ... | 1975 | 1231046 |
| [changes of the inner ear in experimental stapedectomy]. | 1975 | 1230953 | |
| [hearing rehabilitation without a prosthesis following otosclerosis]. | 2005 | 1230951 | |
| [nutrition and taxonomy of "enterobacteriaceae" and related bacteria. iii. nutritional characters and differentiation of the taxonomic groups (author's transl)]. | a batch of 186 strains belonging to the families enterobacteriaceae or vibrionaceae has been studied by determination, for each strain, of the "versatility" towards 146 organic substrates tested as sole source of carbon and energy. this study allowed to work out a classification of these strains into the 32 classes which have been previously described. in the present paper the nutritional characters of these classes are reported. on the basis of these characters, the 32 classes may be regrouped ... | 1975 | 1217784 |
| auto-anti-pra: a 'second' example in a newborn. | a second example of anti-pra was recognized in a newborn infant which exhibited several signs of development disease. the antibody was complement-fixing 19s igm and showed a high thermal range but no detectable haemolytic anaemia was associated. it gave negative reactions with a panel of animal cells as well as with two samples of human en(a-) cells. the latter, however, could absorb the antibody. | 1975 | 1201231 |
| rights and birth by artificial insemination. | 2005 | 1196234 | |
| fish furunculosis caused by aeromonas salmonicida var. achromogenes. | an epizootic of sub-acute furunculosis caused by an acromogenic variety of aeromonas salmonicida is described. bacteriologic, serologic and virulence characteristics of the isolate are presented. | 1975 | 1195489 |
| [letter: adverse effects of antiepileptic drugs]. | 2005 | 1183340 | |
| [carpal tunnel syndrome]. | 2005 | 1183292 | |
| detection of aeromonas salmonicida antigen in diseased fish tissue. | 1975 | 1171156 | |
| proceedings: slow recovery of the inactivation of sodium conductance in myxicola giant axons. | 2005 | 1151857 | |
| balloon obdurator can ease introduction of blunt-tip endoscope. | 2005 | 1140541 |