Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [meningitis caused by acinetobacter calcoaceticus]. | 1982 | 7144321 | |
| [optimization of culture conditions for acinetobacter calcoaceticus grown on n-alkanes in a laboratory fermenter]. | the growth conditions for the cultivation of acinetobacter calcoaceticus in a commercial laboratory fermenter have been optimized. this was done taking former results of shake cultures as a basis. at the end of the logarithmic phase up to 10 g/l dry weight were obtained. the following culture conditions have been used: the medium contained 10 ml/l n-alkane and 6 g/l nh4cl. the ph was adjusted to 6.5 and po2 employed to 70% saturation. a 12 times higher amount of dry weight was observed compared ... | 1982 | 7136011 |
| bacteriuria caused by acinetobacter calcoaceticus biovars in a normal population and in general practice. | a total of 14 strains of acinetobacter calcoaceticus biovar anitratus and 93 strains of a. calcoaceticus biovar lwoffi from a total of 97 patients participating in studies of significant bacteriuria were cultured. these studies involved an interview survey of asymptomatic significant bacteriuria and a multiple-center investigation of urinary tract infection diagnosed in general practice. antibiotic susceptibilities of the strains as determined by a disk diffusion technique were generally in agre ... | 1982 | 7130359 |
| [antibiotic sensitivity of acinetobacter calcoaceticus in patients with infected burns]. | sensitivity of acinetobacter calcoaceticus var. anitratum isolated from infected burn wounds was studied with respect to 24 antibiotics. it was found that the isolates were multiple drug resistant. sensitivity was observed at least to 8 out of 24 antibiotics. the majority of the isolates were resistant to 10--15 drugs. many strains were resistant to 16--17 antibiotics. 11 antibacterial drugs, i. e. tobramycin, sisomicin, gentamicin, amicacin, neomycin, carbenicillin, polymyxin m, erythromycin, t ... | 1982 | 7114831 |
| glucose-nonfermenting gram-negative bacilli associated with clinical veterinary specimens. | glucose-nonfermenting gram-negative bacilli (nfb) have been recognized recently as opportunistic pathogens of humans. with few exceptions, strains of nfb have not been considered important enough to be identified when isolated from animals. in this study, all nfb isolated during a 1-year period in a clinical veterinary microbiology laboratory were identified to determine their prevalence. of the 347 strains of nfb obtained, the most common species were pseudomonas aeruginosa, acinetobacter calco ... | 1982 | 7107835 |
| nosocomial bacteremia due to acinetobacter calcoaceticus. | in 1980, 13 patients with positive blood cultures for acinetobacter calcoaceticus were detected in an 800 bed university medical center. twelve of the 13 isolates were identified as acinetobacter calcoaceticus var. anitratus and one as var. lwoffi. in the same period there were 361 positive specimens of a. calcoaceticus. eight of the patients were classified as having significant bacteremia (group a) with a serious infection in which acinetobacter was considered a significant pathogen. five addi ... | 1982 | 7107014 |
| acinetobacter meningitis: acquired infection in a neonatal intensive care unit. | a cluster of 4 cases of meningitis due to acinetobacter calcoaceticus var anitratus occurred during a 5-day period in a neonatal intensive care unit. three of the infants were preterm and all had a history of other medical problems. initiation of intravenous therapy with carbenicillin was accompanied by clinical recovery and a bacteriological cure. intensive bacteriological investigation failed to show a common source for the infections. | 1982 | 7103551 |
| hand carriage of aerobic gram-negative rods by health care personnel. | a quantitative culture technique (hand washed in a glove containing broth for 30 s) was used to determine the frequency of hand carriage of aerobic gram-negative rods by various groups of health care workers and 104 control subjects. overall, 31% of health care workers carried aerobic gram-negative rods on their hands compared to 59% of control subjects (p < 0.001). enterobacter agglomerans accounted for 40% of the isolates, and other enterobacter spp. 7%. other organisms included acinetobacter ... | 1982 | 7097001 |
| the effects of temperature and ph on the growth of eight enteric and nine glucose non-fermenting species of gram-negative rods. | we studied the heat resistance and the range of growth temperature o gram-negative rods to find one of the bacterial factors governing their infectivity in exogenous and endogenous infections in predisposed patients. escherichia coli, klebsiella pneumoniae, serratia marcescens, pseudomonas aeruginosa, and acinetobacter calcoaceticus grew equally well at 25, 30, 37, and 42 c. among other sugar non-fermenting gram-negative rods, six species showed suppressed growth at either or both ends of the in ... | 1982 | 7087800 |
| [acinetobacter colcoaceticus: its properties, identification key and a study of its role in acute intestinal diseases]. | the authors present their new scheme for the identification of acinetobacter calcoaceticus, requiring a lesser number of test, and the characterstics of 84 strains isolated from patients with acute intestinal diseases and from the environment. some strains are atypical. acinetobacter has been detected in 7.8 +/- 1.0% of patients with acute intestinal diseases. the authors' observations confirm that the detection of acinetobacter in washings from such patients is more convincing for etiological d ... | 1982 | 7080744 |
| weigle reactivation in acinetobacter calcoaceticus. | 1982 | 7071193 | |
| different forms of quinoprotein aldose-(glucose-) dehydrogenase in acinetobacter calcoaceticus. | the ratios of the oxidation rates of aldose sugars, determined in cell-free extracts of acinetobacter calcoaceticus, vary with the strain and growth conditions used. three distinct forms of glucose dehydrogenase with different substrate specificities, occurring in variable proportions in these extracts, are responsible for this effect. one form is the already known "soluble glucose dehydrogenase", the other two forms are complexes containing enzyme and components of the respiratory chain. the pr ... | 1982 | 7065812 |
| isolation, purification, and chemical analysis of the lipopolysaccharide and lipid a of acinetobacter calcoaceticus nctc 10305. | the lipopolysaccharide of acinetobacter calcoaceticus nctc 10305 (london) was obtained by a modified phenol/chloroform/light petroleum method from the bacterial cells and from the culture medium in yields of 1.6% and 2.2% respectively (based on the bacterial dry weight). on chemical analysis, both preparations proved to be identical. the lipopolysaccharide obtained from the cells was purified by repeated ultracentrifugation, electrodialysis, and precipitation with sodium chloride. it was free of ... | 1982 | 7060573 |
| evaluation of the automicrobic system for identification of glucose-nonfermenting gram-negative rods. | with the enterobacteriaceae plus biochemical card, the automicrobic system (vitek systems, inc., hazelwood, mo.) is purported to identify members of the family enterobacteriaceae and seven species of glucose-nonfermenting gram-negative rods: pseudomonas aeruginosa, p. fluorescens, p. putida, p. maltophilia, p. cepacia, and acinetobacter calcoaceticus (saccharolytic and non-saccharolytic). the latter capability was examined in this investigation. of 410 glucose-nonfermenting rods included in the ... | 1982 | 7040451 |
| quinoprotein alcohol dehydrogenase from a non-methylotroph, acinetobacter calcoaceticus. | acinetobacter calcoaceticus grown on ethanol contains an nad(p)+-independent alcohol dehydrogenase which resembles methanol dehydrogenase from methylotrophic bacteria in many respects. likewise, the prosthetic group of this enzyme appears to be identical to that of methanol dehydrogenase, namely, pyrrolo quinoline quinone. the organism is unable to grow on methanol, which means that quinoprotein alcohol dehydrogenases are not restricted to methylotrophs. arguments are presented for the idea that ... | 1981 | 7033448 |
| trimethoprim-sulfamethoxazole therapy for infective endocarditis. | a man with stenosis of the aortic valve acquired endocarditis after abdominal surgery. klebsiella pneumoniae and acinetobacter calcoaceticus were cultured from his blood. the blood cultures remained positive despite intravenous gentamicin and cephalothin to which the organisms were sensitive in vitro. ultimately, the blood was sterilized by a combination of gentamicin and trimethoprim-sulfamethoxazole taken orally. the course of the patient was complicated by cardiac arrest and pericardial tampo ... | 1981 | 7029718 |
| behaviour of bacteriophage mu in acinetobacter calcoaceticus ebf65/65. | transfer of rp4::mu plasmids from escherichia coli k12 to acinetobacter calcoaceticus ebf65/65 was very inefficient compared with rp4 and was only detectable to strains of ebf65/65 lacking pav2, a cryptic plasmid thought to code for a restriction/modification system. rp4::mu-derived plasmids transferred from pav2-strains of ebf65/65 back to e. coli k12 were found to carry defective prophages which had lost the ability to produce detectable phage particles. re-transfer of these defective plasmids ... | 1980 | 7021766 |
| restriction mediated by pav2 affects the transfer of plasmids in acinetobacter calcoaceticus. | the naturally occurring plasmid pav2 restricts the entry of the p class plasmid rp4 and the w class plasmids r388 and s-a into acinetobacter calcoaceticus strain ebf65/65 from escherichia coli. the w class plasmids only transfer from e. coli into pav2-strains. plasmid rp4 is modified in the presence of pav2 such that it is no longer restricted on entry into pav2 recipients of strain ebf65/65. | 1980 | 7021765 |
| solubilization and stabilization of l(+)-mandelate dehydrogenase from acinetobacter calcoaceticus. | 1980 | 7004952 | |
| studies on glucose nonfermentative gram-negative bacilli. ii. transferable drug resistance in acinetobacter calcoaceticus. | 1980 | 7000977 | |
| evaluation of the four-hour micro-id technique for direct identification of oxidase-negative, gram-negative rods from blood cultures. | a 4-h micro-id technique for direct identification of oxidase-negative gram-negative rods from positive blood cultures was compared to subculture and species identification of single colonies by api 20e and micro-id, using standardized inocula. a total of 127 patients (220 positive cultures) were studied. isolates included 96 escherichia coli, 46 klebsiella pneumoniae, 7 klebsiella oxytoca, 8 enterobacter aerogenes, 17 enterobacter cloacae, 19 serratia marcescens, 2 serratia liquefaciens, 8 prot ... | 1980 | 6999020 |
| a case of neonatal meningitis caused by acinetobacter calcoaceticus var anitratus. | 1982 | 6981750 | |
| purification and characterization of rhodanese from acinetobacter calcoaceticus. | rhodanese (thiosulfate : cyanide sulfur transferase, ec 2,8,1,1) was found to be contitutively present as an intracellular enzyme in acinetobacter calcoaceticus. the soluble enzyme was purified 40.9-fold by a procedure which included ultracentrifugation, ethanol precipitation, cm-sephadex batchwise separation, qae-50 ion exchange chromatography, and socrose density gradient ultracentrifugation. the enxyme had a molecular weight of approximately 35000 with a ph optimum of 8-8.5. activity was subs ... | 1980 | 6931629 |
| a common source outbreak of acinetobacter pulmonary infections traced to wright respirometers. | over a 30-day period, acinetobacter calcoaceticus var. antiratus was the responsible pathogen for hospital-acquired pneumonia in 10 patients, and resulted in the colonization of the upper respiratory tract in an additional 9 patients. wright respirometers contaminated by this organism were shown to be the common source for the outbreak as indicated by the recovery of a single serotype (8j), the inability to recover acinetobacter from any other environmental source, and the demonstration that moi ... | 1980 | 6930637 |
| comparative in vitro study of temocillin (brl 17421), a new penicillin. | the activity of temocillin (brl 17421), a new penicillin, was tested in vitro against 653 isolates of gram-negative bacilli and gram-positive cocci. the drug was compared with other beta-lactam antibiotics and tobramycin. it inhibited the majority of gram-negative bacilli tested except for pseudomonas aeruginosa and acinetobacter calcoaceticus, which were highly resistant. it was active against more than 50% of the multiresistant strains tested. temocillin was more active than mezlocillin agains ... | 1982 | 6919412 |
| progressive resistance in a single strain of acinetobacter calcoaceticus recovered during a nosocomial outbreak. | a study was undertaken to investigate a nosocomial outbreak of acinetobacter calcoaceticus. the outbreak occurred in a shock-trauma intensive care unit (icu) during a 6-month period and involved a total of 37 cases. although no deaths were attributable to acinetobacter, 60% of patients from whom acinetobacter was isolated were determined to have a lower respiratory tract infection. a significant difference (p less than 0.05) was noted between the length to stay in the unit of patients who acquir ... | 1982 | 6919395 |
| emulsan production by acinetobacter calcoaceticus in the presence of chloramphenicol. | when exponentially growing cultures of acinetobacter calcoaceticus rag-1 or rag-92 were either treated with inhibitors of protein synthesis or starved for a required amino acid, there was a stimulation in the production of emulsan, an extracellular polyanionic emulsifier. emulsan synthesis in the presence of chloramphenicol was dependent on utilizable sources of carbon and nitrogen and was inhibited by cyanide or azide or anaerobic conditions. radioactive tracer experiments indicated that the en ... | 1982 | 6896872 |
| acinetobacter calcoaceticus--a pathogen? | 1983 | 6886450 | |
| acinetobacter mediastinitis and pneumonia in a thorotrastoma patient. the oropharyngeal flora as source of infection. | a case of bilateral pneumonia, mediastinitis and septicaemia caused by acinetobacter calcoaceticus and candida albicans is described. the infections occurred after a palliative operation for an esophagotracheal fistula in a thorotrastoma patient. the oropharynx was colonized by the two microorganisms at admission and is presented as the source of these infections. clinical management and antimicrobial policy, including oropharyngeal decontamination, leading to a good outcome are reported. | 1983 | 6863724 |
| biological activities of the lipopolysaccharide and lipid a from acinetobacter calcoaceticus. | 1983 | 6842571 | |
| [acinetobacter calcoaceticus strains in the environmental objects of a burn unit]. | 1983 | 6840587 | |
| [bacteria of the species acinetobacter calcoaceticus as the possible etiological factor in infectious complications in burns]. | the results of the study of the etiological role of acinetobacter calcoaceticus in infectious complications of burning lesions are presented. these bacteria were shown to occur in 28% of burn wounds, quite frequently as a monoculture or association with diphtheroids. a. calcoaceticus were capable of persisting in the wounds during the whole period of treatment (up to 2-3 months). a high level of the contamination of burn wounds with these microorganisms, reaching 10(5)-10(6) cells per 1 g of tis ... | 1983 | 6837196 |
| reversible effects of cross-linking on the regulatory cooperativity of acinetobacter citrate synthase. | citrate synthase was purified from acinetobacter calcoaceticus and treated with the cleavable cross-linking reagent dithiobis(succinimidyl propionate). cross-linking of the enzyme resulted in the abolition of the sigmoidal responses to inhibition by nadh and re-activation by amp displayed by the native enzyme. inhibition and re-activation were still observed but without any cooperativity. cleavage of the disulphide bonds in the cross-links by treatment with dithiothreitol restored the sigmoidal ... | 1983 | 6832355 |
| outbreak of pyrogenic reactions at a dialysis center. association with infusion of heparinized saline solution. | twenty-three pyrogenic reactions occurred in 16 patients undergoing hemodialysis at a private dialysis center in the south central united states between november 23 and december 2, 1978. no deaths were attributed to reactions; however, 10 patients were hospitalized for observation after experiencing a reaction. cultures of all blood specimens obtained from the patients gave negative results. chills (75 percent), nausea and/or vomiting (30 percent), and fever (90 percent) were the most common sig ... | 1983 | 6829590 |
| [studies on acinetobacter calcoaceticus. protective effects of antiserum and vaccine to infection]. | 1982 | 6816861 | |
| [studies on acinetobacter calcoaceticus. the virulence and the therapeutic efficacy of antibiotics in mice]. | 1982 | 6816860 | |
| the isolation of cell surface mutants of acinetobacter calcoaceticus rag-1. | the isolation of cell surface mutants of acinetobacter calcoaceticus rag-1 was demonstrated by a method based on the differential immunoprecipitating properties of wild-type cells and mutants. in the presence of antiserum, about 99% of wild-type cells in suspension sedimented following overnight agglutination. the kinetics of antiserum-induced agglutination was quantified by measuring the time-dependent decrease in turbidity. for mutant isolation, a sample of the cells remaining in the supernata ... | 1981 | 6806434 |
| [rubredoxin reductase in crude extracts of acinetobacter calcoaceticus in relation to carbon source and growth phase]. | by immunization of rabbits with discelectrophoretically purified rubredoxin-reductase from ac. calcoaceticus an antiserum against this enzyme was prepared. the antiserum was monospecifical to a diaphoretic activity, which had a rf-value identical with the rubredoxin-reductase-rf value. it has been shown by discelectrophoresis, immunoelectrophoresis, ouchterlony technique and kinetic investigations that crude extracts of bacteria grown on c16, malate, succinate or acetate contain rubredoxin-reduc ... | 1982 | 6803449 |
| acinetobacter meningitis - a diagnostic pitfall. a report of 3 cases. | three fatal cases of meningitis caused by acinetobacter calcoaceticus var. anitratus are reported, 1 in a premature infant who was being artificially ventilated and 2 in adults who had had neurosurgical operations. the microscopic resemblance of this organism to neisseria meningitidis is high-lighted and the problems of antimicrobial therapy are discussed. | 1982 | 6801786 |
| transfer of the gonococcal penicillinase plasmid: mobilization in escherichia coli by incp plasmids and isolation as a dna-protein relaxation complex. | a 4.4-megadalton penicillinase plasmid, pwd2, from neisseria gonorrhoeae was transformed into escherichia coli. pwd2 was efficiently mobilized by incp plasmids in e. coli but not by flac, r1drd-19, or r64drd-11. pwd2 could be isolated as a dna-protein relaxation complex with properties similar to the well characterized cole1 complex. the host range of pwd2 was shown to include gonococci, enterobacteriaceae, and hemophilus influenzae, but not acinetobacter calcoaceticus or pseudomonas aeruginosa. ... | 1982 | 6801022 |
| bb-k 122: in vitro and in vivo activity against ocular pathogens. | bb-k 122 is a new aminoglycoside antibiotic and an analogue of amikacin. this study evaluated the in vitro and in vivo activity of bb-k 122 and gentamicin against important ocular pathogens. bb-k 122 and gentamicin demonstrated generally equivalent in vitro antibacterial activity, except that gentamicin was more active against streptococcus sp. bb-k 122 showed significant in vitro activity against important ophthalmic pathogens, including pseudomonas aeruginosa, staphylococcus aureus, acinetobac ... | 1981 | 6797791 |
| microbial colonization of prosthetic devices. ii. scanning electron microscopy of naturally infected intravenous catheters. | forty two nonselected naturally infected intravenous catheters were investigated by scanning electron microscopy (sem) and usual bacteriological methods. in many catheter samples an amorphous deposited substance could be detected by sem investigation, mostly associated with the isolation of staphylococci, acinetobacter calcoaceticus and pseudomonas aeruginosa. the thickest layers of such a substance were found in catheters infected by coagulase-negative staphylococci. the bacteria seemed to be c ... | 1981 | 6792814 |
| selective nonroutine microbial surveillance of in-use hospital nebulizers by aerosol entrapment and direct sampling analyses of solutions in reservoirs. | effluent aerosols and liquid reservoir samples from 255 in-use hospital nebulization devices were analyzed by aero-test samplers (olympic corp.) and direct dilution procedures (0.1-, 0.01-, and 0.001-ml plated samples). thirty-five percent (89 of 255) of the in-use hospital reservoir samples were positive by direct dilution, and 24% (61 of 255) were positive by aero- test samplers. acinetobacter calcoaceticus var. anitratus was found either alone or in association with pseudomonas spp. in 50% of ... | 1980 | 6785304 |
| evolutionary relationships among gamma-carboxymuconolactone decarboxylases. | gamma-carboxymuconolactone decarboxylase (ec 4.1.1.44) from azotobacter vinelandii resembled the isofunctional enzymes from acinetobacter calcoaceticus and pseudomonas putida. all three decarboxylases appeared to be hexamers formed by association of identical subunits of about 13,300 daltons. the a. vinelandii and p. putida decarboxylases cross-reacted immunologically with each other, and the nh2-terminal amino acid sequences of the enzymes differed in no more than 7 of the first 36 residues. in ... | 1981 | 6783616 |
| evolutionarily homologous alpha 2 beta 2 oligomeric structures in beta-ketoadipate succinyl-coa transferases from acinetobacter calcoaceticus and pseudomonas putida. | homogeneous beta-ketoadipate succinyl-coa transferase (ec 2.8.3.6) preparations were obtained from extracts of acinetobacter calcoaceticus and pseudomonas putida. gel filtration indicated that the respective transferases have similar molecular weights of 108,000 and 109,000; each transferase appears to have an alpha 2 beta 2 oligomeric structure formed by association of nonidentical subunits with a molecular weight of about 25,000. the subunits were separated by sodium dodecyl sulfate-gel electr ... | 1981 | 6780551 |
| antimicrobial susceptibility of acinetobacter calcoaceticus, serratia marcescens, pseudomonas fluorescens and pseudomonas maltophilia to three novel cephalosporin-like antibiotics. | 1980 | 6777362 | |
| intergeneric evolutionary homology revealed by the study of protocatechuate 3,4-dioxygenase from azotobacter vinelandii. | protocatechuate 3,4-dioxygenase (ec 1.13.1.3) was purified to homogeneity from extracts of azotobacter vinelandii. the molecular weight of the oligomeric protein was estimated to be 510 000 by gel filtration and 480 000 by ultracentrifugation. the oligomer appears to be formed by association of equal amounts of nonidentical subunits which were estimated by sodium dodecyl sulfate gel electrophoresis to have respective molecular weights of 23 300 and 25 250. ten gram-atoms of iron was associated w ... | 1980 | 6766312 |
| fingerprinting acinetobacter strains from clinical sources by numerical analysis of electrophoretic protein patterns. | a total of 57 strains of acinetobacter calcoaceticus was fingerprinted by sds-page of cellular protein. all strains were also examined by conventional, api and n/f-tek methods, and antibiotic sensitivity patterns were determined. in general, using the api 20e and n/f-tek methods, it was possible to assign isolates of a. calcoaceticus to the two accepted "biotypes" or "varieties", a.c. anitratus and a.c. lwoffi, but these methods did not offer useful subdivision of the biotypes. gel electrophores ... | 1984 | 6748041 |
| in vitro activity of apalcillin compared with those of piperacillin and carbenicillin against 6,797 bacterial isolates from four separate medical centers. | quantitative susceptibility tests were performed in four separate medical centers, in which apalcillin was compared with piperacillin and carbenicillin. data from tests of 6,797 isolates confirmed that apalcillin and piperacillin had nearly identical spectra of activity but that apalcillin was significantly more active against pseudomonas aeruginosa (mic required to inhibit 90% of strains, 2.0 versus 64 micrograms/ml) and acinetobacter calcoaceticus subsp. anitratus (mic required to inhibit 90% ... | 1984 | 6732234 |
| not gliding but twitching motility of acinetobacter calcoaceticus. | 1984 | 6707215 | |
| bacterial degradation of emulsan. | emulsan is a polyanionic heteropolysaccharide bioemulsifier produced by acinetobacter calcoaceticus rag-1. a mixed bacterial population was obtained by enrichment culture that was capable of degrading emulsan and using it as a carbon source. from this mixed culture, an emulsan-degrading bacterium, termed yuv-1, was isolated. strain yuv-1 is an aerobic, gram-negative, non-spore-forming, rod-shaped bacterium which grows best in media containing yeast extract. when placed on preformed lawns of a. c ... | 1983 | 6688940 |
| enzymatic depolymerization of emulsan. | emulsan, the polyanionic emulsifying agent synthesized by acinetobacter calcoaceticus rag-1, was depolymerized by an enzyme obtained from a soil bacterium yuv-1. the extracellular emulsan depolymerase was produced when strains rag-1 and yuv-1 were grown together on agar medium. the enzyme was extracted from the agar and concentrated by ultrafiltration and ammonium sulfate precipitation. the molecular weight of the enzyme was estimated to be 89,000. emulsan depolymerase activity was due to an eli ... | 1983 | 6688620 |
| immunochemical identification of the major cell surface agglutinogen of acinetobacter calcoaceticus rag-92. | the immunochemical and immunocytochemical characteristics of three acinetobacter calcoaceticus rag strains were compared in order to clarify the relationship between antibody-induced agglutination and the production of polyanionic extracellular emulsifier (termed emulsan). in addition to the parent, rag-92, two mutant strains were examined: (1) a non-agglutinating emulsan-producer (ab15), and (2) an agglutinating mutant (16tlu) defective in the production of emulsan. a combined genetic-immunoche ... | 1983 | 6688443 |
| localization of emulsan-like polymers associated with the cell surface of acinetobacter calcoaceticus. | various immunochemical techniques were employed to probe the relationship between the extracellular emulsifying agent (emulsan) and the cell-associated form of the polymer in acinetobacter calcoaceticus rag-1. using an emulsan-specific antibody preparation, immunocytochemical labeling revealed that an emulsan-like antigen is a major component of the 125-nm minicapsule which envelopes the exponential-phase cell of the parent strain. the marked reduction of this capsule in stationary-phase cells w ... | 1983 | 6687725 |
| bacteria-immune system interactions. ii. adherence of acinetobacter calcoaceticus strains to human lymphocytes. | out of twenty-nine strains of acinetobacter calcoaceticus (a. calcoaceticus) only eight adhered to lymphocytes. the percentage of lymphocytes labeled with bacteria varied from 5 to 70%. most of the labeled lymphocytes had large numbers of bacteria attached to them. the variation in the percentages of lymphocytes labeled in various individuals was minimal. | 1983 | 6642551 |
| modulation of isocitrate dehydrogenase activity in acinetobacter calcoaceticus by acetate. | the addition of acetate to a culture of acinetobacter calcoaceticus grown in medium containing limiting succinate as the sole carbon and energy source leads to an increase in the specific activity of isocitrate dehydrogenase. this is in contrast to similar studies with several other microorganisms in which acetate induces an atp-dependent phosphorylation and concomitant decrease in the specific activity of this enzyme. | 1983 | 6641942 |
| in vitro activities of new beta-lactam antibiotics against acinetobacter spp. | the in vitro activities of new beta-lactam antibiotics was studied and compared with those of other known agents against 51 clinical isolates of acinetobacter calcoaceticus subsp. anitratus and 23 isolates of a. calcoaceticus subsp. lwoffi. of the new beta-lactam antibiotics, imipemide (n-formimidoyl thienamycin), ceftazidime, ceftizoxime, ceftriaxone, and piperacillin demonstrated good activities. the minimal inhibitory concentrations for a. calcoaceticus subsp. lwoffi were lower than those obt ... | 1983 | 6638992 |
| acinetobacter calcoaceticus infection in and around manipal (karnataka). | 1983 | 6605922 | |
| n-formimidoyl thienamycin: in vitro comparison with cefoxitin and tobramycin against clinical, bacterial isolates. | the in vitro activity of the novel beta-lactam antibiotic, n-formimidoyl thienamycin (n-f thienamycin) has been compared with those of cefoxitin and tobramycin. an agar dilution method was employed. n-f thienamycin was active against all enterobacteriaceae isolates (mic less than or equal to 4 mg/l). all pseudomonas aeruginosa isolates were inhibited by 2 mg/l. n-f thienamycin was also active against acinetobacter calcoaceticus (96 per cent inhibited by 0.5 mg/l) and gram-positive cocci. all ent ... | 1983 | 6603093 |
| nucleotide sequence of the acinetobacter calcoaceticus trpgdc gene cluster. | a plasmid library of acinetobacter calcoaceticus hindiii fragments was constructed, and clones that complemented an escherichia coli paba mutant were selected. plasmids containing a 3.9-kb fragment of a. calcoaceticus dna that also complemented e. coli trpd and trpc-(trpf+) mutants were obtained. we infer that complementation of e. coli paba mutants was the result of the expression of the amphibolic anthranilate-synthase/p-aminobenzoate-synthase glutamine-amidotransferase gene and that the plasm ... | 1984 | 6599977 |
| enoxacin: worldwide in-vitro activity against 22451 clinical isolates. | a worldwide multicentre study involving 31 centres in eight countries was conducted to compare the in-vitro activity of enoxacin with that of other currently available antibiotics. enoxacin was active against virtually all of the species of enterobacteriaceae tested (mic90 less than or equal to 0.25-2 mg/l). it was also effective at inhibiting pseudomonas aeruginosa (mic90 = 2 mg/l; n = 3540), staphylococcus aureus and epidermidis (mic90 = 2 mg/l; n = 2635 and 837 resp.), acinetobacter calcoacet ... | 1984 | 6594322 |
| p-chloromercuribenzoate specifically modifies thiols associated with the active sites of beta-ketoadipate enol-lactone hydrolase and succinyl coa: beta-ketoadipate coa transferase. | beta-ketoadipate enol-lactone hydrolase (ec 3.1.1.24) and succinyl coa: beta-ketoadipate transferase (ec 2.8.3.6) catalyze consecutive metabolic reactions in bacteria. the enzymes appear to be members of different families of related proteins. enzymes within the enol-lactone hydrolase family appear to have diverged so extensively that common ancestry sometimes is not directly evident from comparison of nh2-terminal amino acid sequences of the proteins. amino acid sequences at or near the active ... | 1984 | 6591865 |
| taxonomic implications of quantitative transformation in acinetobacter calcoaceticus. | quantitative transformation of streptomycin resistance marker was carried out with strains of acinetobacter calcoaceticus. standard recipient strain was the competent bd4-ss. transformation proceeded in a liquid system with a concentration of 20 micrograms/ml of streptomycin resistant dna (sr-dna) from bd4, 17 reference strains of acinetobacter, and 42 recent clinical isolates of the acid forming variant (anitratus) and 12 of the non-acid forming variant (lwoffi) as donors of sr-dna. the exposur ... | 1983 | 6586037 |
| factors affecting quantitative transformation of streptomycin resistance markers in acinetobacter calcoaceticus. | quantitative transformation of acinetobacter calcoaceticus bd4-ss exposed to autologous dna in a liquid system was examined with streptomycin resistance as a marker. the exposure to dna was terminated by addition of dnase at set intervals. the variables tested were concentrations of autologous sr-dna, exposure time, growth phase, recipient cell density, and the ph of the liquid transformation system. the ratio of transformants to total number of recipient cells (t/r-ratio) was proportional to th ... | 1983 | 6586036 |
| meningitis with acinetobacter calcoaceticus in cerebrospinal fluid. a case report. | a case of meningitis due to acinetobacter calcoaceticus occurred after neurosurgery. the cerebrospinal fluid cytology showed intracellular diplococci that strongly resembled neisseria meningitidis. however, subsequent bacteriologic studies revealed a bacterium identical to a. calcoaceticus. it is of practical importance for cytology laboratories to recognize this diplococcal form of organism. | 1983 | 6575544 |
| direct dilution sampling, quantitation, and microbial assessment of open-system ventilation circuits in intensive care units. | in a systematic approach, 37 duplicate samples of open system circuits (bennett ma-1 ventilators) of patients in medical and surgical intensive care units were processed by direct and serial (apha guidelines) dilutions. the paired difference test on 15 of the in-use circuitry solution samples indicated no difference between the direct and serial dilution methods (p less than 0.001). seventy-seven additional respiratory therapy circuitry samples from similar intensive care patients were analyzed ... | 1983 | 6575015 |
| specific binding of a bacteriophage at a hydrocarbon-water interface. | emulsan, the extracellular polyanionic emulsifying agent produced by acinetobacter calcoaceticus rag-1, has been implicated as a receptor for a specific virulent rag-1 bacteriophage, ap3. aqueous solutions of emulsan did not interfere with phage ap3 adsorption to rag-1 cells. however, binding of phage ap3 occurred at the interfaces of hexadecane-in-water emulsions specifically stabilized by emulsan polymers. binding of ap3 to emulsions was inhibited either in the presence of anti-emulsan antibod ... | 1984 | 6546308 |
| [acinetobacter calcoaceticus, a potential pathogen with an increasing role in pathology]. | 1984 | 6522946 | |
| phage-types and susceptibility to 26 antibiotics of nosocomial strains of acinetobacter isolated in portugal. | sixty-two strains of acinetobacter calcoaceticus isolated from pathological samples or from the environment in several hospitals in lisbon, were studied by means of two complementary phage-typing systems. eighteen phage-types or sub-types, one group of uncommon types (9.6%) and one group of untypable strains (20.9%) were found. a new phage-type (no. 104) and a new sub-type (no. 18) were defined among the portuguese strains. the in vitro activity of 26 antibiotics against 48 nosocomial strains wa ... | 1984 | 6519351 |
| effect of antibiotic use on the incidence of cephalosporin resistance in two australian hospitals. | the incidence of resistance of gram-negative bacteria to three generations of cephalosporins was surveyed in two large hospitals with widely differing rates of cephalosporin usage. overall resistance (mic greater than 5 mg/l) of enterobacteriaceae to cefotaxime in the hospital using large amounts of cephalosporins was 4% compared with 0.7% in the other. enterobacter species accounted for most resistant isolates and resistant enterobacter cloacae replaced sensitive strains in four patients given ... | 1984 | 6501136 |
| evaluation of the rapid nft system for identification of gram-negative, nonfermenting rods. | this study evaluated the ability of the rapid nft system (api system sa, montalieu-vercieu, france) to accurately identify 262 clinically isolated, gram-negative, nonfermentative rods without additional tests. identifications were classified as correct; low discrimination, with a spectrum of two or more possibilities (additional tests necessary for accurate identification); and incorrect. correct identification rates were analyzed in two categories: (i) correct to species or biotype for all orga ... | 1984 | 6490857 |
| characterization of an antibiotic resistance plasmid pav5 and its constituent replicons in acinetobacter calcoaceticus. | 1984 | 6489752 | |
| inhibitory and bactericidal activities of amifloxacin, a new quinolone carboxylic acid, compared with those of seven other antimicrobial agents. | the activity of amifloxacin, a new quinolone carboxylic acid compound, against 147 strains of microorganisms was studied and was compared with the activities of cinoxacin, trimethoprim, amikacin, and four beta-lactam antimicrobials. the minimal concentration at which 90% of strains were inhibited by amifloxacin was lowest for escherichia coli and klebsiella sp (less than or equal to 0.125 microgram/ml), followed by proteus sp (less than or equal to 0.25 microgram/ml), enterobacter sp and citroba ... | 1984 | 6440708 |
| in vitro antibacterial properties of brl 36650, a novel 6 alpha-substituted penicillin. | brl 36650 is a new type of penicillin in which a formamido group has been introduced into the 6 alpha-position of the nucleus. the compound is highly active against aerobic gram-negative bacteria and is stable to a wide range of beta-lactamases produced by these organisms. against members of the family enterobacteriaceae, brl 36650 was considerably more active than piperacillin, particularly against beta-lactamase-producing strains, and showed a similar level of activity to moxalactam, aztreonam ... | 1984 | 6440479 |
| comparative in vitro activities of apalcillin and piperacillin against gram-negative bacilli. | the susceptibilities of 317 gram-negative bacilli to apalcillin and piperacillin were determined by standardized microdilution and disk diffusion tests. the respective percentages of strains susceptible to less than or equal to 64 micrograms of apalcillin and piperacillin per ml were as follows: members of the family enterobacteriaceae, 90 and 88%; randomly selected pseudomonas aeruginosa, 100 and 100%; multi-drug-resistant p. aeruginosa, 100 and 75%; and other nonfermenters and aeromonas hydrop ... | 1984 | 6440476 |
| postoperative ocular infections: an analysis of laboratory data on 750 cases. | from 1981-1982, 750 clinical specimens from patients who developed postoperative infections were processed in the ocular microbiology laboratory. bacterial cultures were positive in 71.0%, fungal in 1.3% and another 1.4% yielded both. the remaining 26.3% were sterile. infections were due chiefly to gram positive organisms (63.6%); only 6.4% were due to gram negative bacteria while 0.9% were due to both. staphylococcus aureus (52.0%) was the most common isolate, followed by s epidermidis (37.2%). ... | 1984 | 6439098 |
| in-vitro activity of enoxacin against aminoglycoside-resistant gram-negative bacilli and other clinical isolates. | the in-vitro activity of enoxacin was tested against 500 clinical isolates of gram-negative bacilli that were resistant to one or more of gentamicin, tobramycin and amikacin, and against 1060 recent consecutive clinical isolates of gram-negative bacilli and gram-positive cocci. enoxacin was active against staphylococci (mics less than or equal to 4 mg/l) but less active against streptococcus faecalis (mics mostly 8 mg/l). it was active against pseudomonas aeruginosa (mics 0.5-4 mg/l) and very ac ... | 1984 | 6438046 |
| nature and location of amide-bound (r)-3-acyloxyacyl groups in lipid a of lipopolysaccharides from various gram-negative bacteria. | it has previously been demonstrated [eur. j. biochem. 124, 191-198 (1982) and 137, 15-22 (1983)] that the lipid a component of salmonella and proteus lipopolysaccharides contains amide-linked (r)-3-acyloxyacyl residues. in the present study lipid a of other gram-negative bacteria was analysed for the presence of amide-bound 3-acyloxyacyl residues. it was found that such residues are constituents of all lipid a tested (agrobacterium tumefaciens, chromobacterium violaceum, pseudomonas aeruginosa, ... | 1984 | 6437812 |
| immunologically related ketodeoxyoctonate-containing structures in chlamydia trachomatis, re mutants of salmonella species, and acinetobacter calcoaceticus var. anitratus. | the lipopolysaccharides (lps) of chlamydia trachomatis, acinetobacter calcoaceticus var. anitratus, and re mutants of salmonella sp. were shown to share related immunodeterminants , as demonstrated by double immunodiffusion and immunoblotting from sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. the cross-reactive material in the extracellular slime of a. calcoaceticus var. anitratus was shown to be released lps. the acinetobacter lps was found to separate in sodium dodecyl sulfat ... | 1984 | 6427112 |
| bacteriological analysis of water by potentiometric measurement of lipoic acid reduction: preliminary assays for selective detection of indicator organisms. | the practical task of adapting an original potentiometric technique to the bacteriological analysis of water is discussed. various laboratory strains of organisms belonging to the usual aquatic flora were inoculated one by one in a minimal lactose broth supplied with lipoic (thioctic) acid. the time evolution of the redox potential of the cultures was followed during incubation by combined gold versus reference electrodes. when the incubation temperature was regulated at 36 degrees c, most organ ... | 1984 | 6421230 |
| isolation, characterization and mapping of mandelate pathway mutants of acinetobacter calcoaceticus. | mutants of acinetobacter calcoaceticus ebf 65/65 that could not grow on intermediates of the mandelate or benzyl alcohol pathways were isolated and in some cases the enzymic lesions were identified. several catabolic markers were mapped using the plasmid pav1. the mandelate genes appeared to be clustered near the auxotrophic marker phe-1 but were not all contiguous with each other. the gene responsible for the appearance of the novel l(+)-mandelate dehydrogenase appeared to be close to a gene re ... | 1984 | 6396377 |
| direct identification and susceptibility testing of gram-negative bacilli from bactec bottles by use of the ms-2 system with updated bacterial identification software. | the abbott ms-2 system (abbott laboratories, diagnostic division, irving, tex.), equipped with updated bacterial identification software (version 03.02), was used to perform both direct identification and susceptibility tests on gram-negative bacilli from positive bactec blood culture bottles. ninety-eight of 101 enterobacteriaceae strains, one strain of acinetobacter calcoaceticus, and two strains of pseudomonas aeruginosa were correctly identified by following a direct inoculation procedure. o ... | 1984 | 6394624 |
| serotyping of acinetobacter calcoaceticus. | serotyping of acinetobacter calcoaceticus by direct immunofluorescence and a capsule swelling reaction is described. one hundred isolates, including 12 from an outbreak in a neonatal department, were studied. ninety five per cent of the isolates were typable by immunofluorescence and could be divided into 30 separate types, but 42.1% of typable strains, including 11 from the outbreak, were of one type. typing results by the capsular swelling reaction generally followed those of immunofluorescenc ... | 1984 | 6392349 |
| [isolation of the l forms of acinetobacter calcoaceticus var. lwoffii in infectious endocarditis]. | in the bacteriological study of blood samples taken from a patient with infectious endocarditis the hemoculture of the l-forms of a. calcoaceticus, var. lwoffii, was isolated. this hemoculture reverted into the bacterial form in the process of subculturing on solid media. the species of the isolated l-forms was established on the basis of the data obtained in the study of the biological properties of the revertants. to ensure the effectiveness of bacteriological investigations, a special search ... | 1984 | 6380163 |
| [bioenzymatic and lysotypic study of 200 strains of acinetobacter calcoaceticus]. | two hundred strains of acinetobacter calcoaceticus isolated between 1971 and 1978 at the bichat hospital were studied by means of a biochemical and enzymatic technique thanks to 150 tests per strain. most strains (about 60%) were acinetobacter calcoaceticus variety anitratus which is more frequently isolated than var. lwoffi . three bioenzymatic groups have been statistically defined. this classification corresponds to the main lines of previous descriptions by henriksen (1960) and those indicat ... | 1984 | 6371676 |
| ceftazidime: in vitro comparison with cephalothin, cefuroxime, and netilmicin. a norwegian study. | the in vitro activity of ceftazidime has been compared with those of cephalothin, cefuroxime, and netilmicin against a variety of gram-positive and gram-negative bacteria in order to register sensitivity patterns in the western part of norway. an agar dilution method was used for minimal inhibitory concentration (mic) determination. ceftazidime was the most active agent against enterobacteriaceae; all isolates being inhibited by 2 mg/l or less, whereas netilmicin was slightly less active, especi ... | 1984 | 6365480 |
| [clinical importance of acinetobacter calcoaceticus isolations from blood and venous catheters]. | from 1980 to 1982 acinetobacter calcoaceticus (a.c.) derived from blood cultures and venous catheters of 29 patients could be grown. 6 patients with clinical signs of gram-negative septicemia had two or more blood cultures positive for a.c. thus, the role of a.c. as the etiologically relevant pathogen seems to be evident. in 14 cases with clinical signs of septicemia but only one set of blood cultures positive for a.c. it is felt to be the causative agent since no other cause could be found. in ... | 1983 | 6362283 |
| transferable plasmid-mediated antibiotic resistance in acinetobacter. | acinetobacter calcoaceticus strain bm2500 was resistant to ampicillin, aminoglycoside-aminocyclitols, chloramphenicol, sulfonamides, and high levels of trimethoprim. resistance to ampicillin was due to the presence of a beta-lactamase (tem-1) and the aminoglycoside-aminocyclitol resistance was mediated by phosphotransferase (aph(3')(5")i) and adenylyltransferase (aad(3)(9] activities. the resistance genes were carried by a 167 kilobase plasmid, pip1031, belonging to incompatibility group 6-c; th ... | 1983 | 6356187 |
| regulation of expression of novel mandelate dehydrogenases in mutants of acinetobacter calcoaceticus. | wild-type strains of acinetobacter calcoaceticus able to grow on only l(+)- or d(-)-mandelate gave rise to mutants that could grow on the other isomer of mandelate. each mutant contained an additional mandelate dehydrogenase which was not expressed in the parent strain. the novel enzymes were shown to be controlled co-ordinately with the pre-existing enzymes for the conversion of mandelate into benzoate when induced with phenylglyoxylate, gratuitously induced with thiophenoxyacetate, subjected t ... | 1983 | 6355386 |
| [comparative in vitro activity of 6 recent beta-lactams against hospital strains of acinetobacter calcoaceticus: role of the monobactam sq 26,776]. | acinetobacter calcoaceticus is recognized as one of the most resistant nosocomial pathogens. clinical isolates of acinetobacter are usually resistant to most beta-lactam antibiotics and even to carbenicillin; 50% of the strains isolated in 1981-82 at the bichat hospital were inhibited only at a concentration of 180 micrograms/ml. recently several new molecules belonging to the beta-lactam group were discovered. among them, monobactam sq 26 776, a new broad spectrum highly potent monocyclic beta- ... | 1983 | 6353329 |
| acinetobacter contamination of laboratory dental pumice. | micro-organisms of the genus acinetobacter, implicated as opportunistic pathogens, have been recovered from dentures after laboratory repair. a study was undertaken to determine if acinetobacter could be isolated from used dental pumice. cultural studies demonstrated that acinetobacter calcoaceticus variety lwoffi was present in high numbers in used pumice and was a major gram-negative microbial contaminant. | 1983 | 6352756 |
| [identification of gram-negative nonfermentation bacteria in the air of a surgical hospital]. | the method of detecting gram-negative nonfermentative bacteria in the air of surgical hospitals by means of the -1 apparatus has been developed. 167 strains of nonfermentative bacteria have been isolated and studied; of these, acinetobacter calcoaceticus constitute 88%. nonfermentative bacteria occur most frequently in the air of wards for patients with severe burns, as well as in the air of dressing rooms when patients with burns are being bandaged there. the possibility of the spread of nonfer ... | 1983 | 6351508 |
| evaluation of the updated ms-2 bacterial identification system in comparison with the api 20e system. | the recently updated ms-2 bacterial identification system software (abbott laboratories, diagnostic division, irving, tex.) was compared with the original ms-2 bacterial identification software and the api 20e, using 968 strains of enterobacteriaceae. the updated ms-2 software correctly identified 94.4% of the isolates tested. api 20e and the original ms-2 software correctly identified 91 and 85.3% of the strains, respectively. ms-2 responses were considered to be equivocal (needing additional t ... | 1983 | 6350342 |
| mutant strains of acinetobacter calcoaceticus possessing additional mandelate dehydrogenases. identification and preliminary characterization of the enzymes. | acinetobacter calcoaceticus wild-type strain n.c.i.b. 8250 can grow on only the l(+)-isomer of mandelate but mutant strains have been isolated that can grow on d(-)-mandelate. these mutants contain a novel d(-)-mandelate dehydrogenase in addition to the original l(+)-mandelate dehydrogenase. a second wild-type strain, ebf 65/65, shows the opposite pattern and can grow on d(-)-mandelate but not on l(+)-mandelate; mutants have been isolated that possess an l(+)-mandelate dehydrogenase in addition ... | 1983 | 6342613 |
| a new lipopolysaccharide antigen identified in acinetobacter calcoaceticus: occurrence of widespread natural antibody. | serological investigation of the lipopolysaccharide (lps) of acinetobacter calcoaceticus revealed a new antigen to which antibody in high titre is present in the serum of many mammalian species. the passive haemolysis test showed that antibody, in titres ranging from 32-4096, was invariably present in the serum of mice, rats, guinea-pigs, and horses. rabbits and human beings had lower and more variable titres (less than 2-512). the antigen persisted after prolonged hydrolysis of the lps in 1% ac ... | 1983 | 6341597 |
| inhibition of bacterial adherence to hydrocarbons and epithelial cells by emulsan. | acinetobacter calcoaceticus rag-1 and bd413, as well as streptococcus pyogenes m-5, adhered to octane. adherence was inhibited by emulsan (100 micrograms/ml), the polymeric emulsifying agent produced by a. calcoaceticus rag-1. emulsan also inhibited adherence of s. pyogenes and rag-1 to buccal epithelial cells. the mean values of bound s. pyogenes per epithelial cell were 57.2 and 20.7 for the control and emulsan-containing suspensions, respectively; mean values of bound rag-1 per epithelial cel ... | 1983 | 6341225 |
| [long-term preservation of reference cultures of microorganisms in the form of gelatin disks]. | the survival of the various bacteria, yeast and fungal species maintained in gelatinous disks for 1-2 years has been tested. as a rule, cultures with poor lyophilization performance were badly preserved in gelatinous disks (e.g. non-sporulating fungi and certain bacteria and yeast species). acinetobacter calcoaceticus and candida requinyii species after being stored for two years do not change the main taxonomic characteristics and possess the growth ability. | 1983 | 6340744 |
| proteolysis of acinetobacter citrate synthase by subtilisin. | citrate synthase from acinetobacter calcoaceticus was subjected to proteolysis with subtilisin. although the enzyme proved relatively resistant to inactivation by this treatment, sds-polyacrylamide gel electrophoresis clearly revealed breakdown of the citrate synthase to smaller fragments. the regulatory responses of the native enzyme to inhibition by nadh and re-activation by amp were retained on proteolysis, indicating that the fragments bind tightly to each other and preserve the overall coop ... | 1983 | 6339274 |
| susceptibility of gram-negative bacteria to polymyxin b nonapeptide. | subinhibitory concentrations of polymyxin b nonapeptide sensitized all 21 polymyxin-susceptible gram-negative bacterial strains studied to hydrophobic antibiotics such as fusidic acid, novobiocin, and erythromycin. the susceptibility increases were usually 30- to 300-fold. the strains included representatives of escherichia coli with different o- and k-antigens, klebsiella pneumoniae, klebsiella oxytoca, enterobacter cloacae, enterobacter agglomerans, salmonella typhimurium, acinetobacter calcoa ... | 1984 | 6331296 |
| transposon tn5 encodes streptomycin resistance in nonenteric bacteria. | strains of caulobacter crescentus, pseudomonas putida, acinetobacter calcoaceticus, rhizobium meliloti, and rhodopseudomonas sphaeroides carrying the kanamycin resistance-encoding transposon tn5 were 15 to 500 times more resistant to streptomycin than transposon-free strains. the streptomycin resistance determinant, which is separable from the kanamycin resistance determinant of tn5, was not expressed in escherichia coli or klebsiella aerogenes. | 1984 | 6330041 |