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microbial products from probiotic bacteria inhibit salmonella enteritidis 857-induced il-8 synthesis in caco-2 cells.oral administration of lactobacillus spp. as probiotics is gaining importance in the treatment of intestinal inflammations. however, their mechanism of action is unknown. we investigated whether nonspecific binding lactobacillus casei shirota (lcs) and mannose-specific lactobacillus plantarum 299v (lp) and their spent culture supernatant (scs) affect salmonella enteritidis 857 (se) growth, il-8 and hsp70 syntheses. in one set of experiments human enterocyte-like caco-2 cells were infected with l ...201020680581
images in cardiovascular medicine: emphysematous aortitis. 201020679577
effect of delivery method on the efficacy of salmonella vaccination in chickens.to investigate whether the efficacy of live vaccines is influenced by the mode of vaccine delivery, a widely-used uk live commercial salmonella enteritidis vaccine was delivered to pullet chicks either by spray, in drinking water, or in combination with a bivalent vaccine containing inactivated salmonella enteritidis and salmonella typhimurium. the birds were subsequently challenged with 10(2) or 10(8) colony-forming units (cfu) of salmonella enteritidis through drinking water at either six or 2 ...201020675624
microarray analysis of response of salmonella during infection of hla-b27- transfected human macrophage-like u937 cells.human leukocyte antigen (hla)-b27 is strongly associated with the development of reactive arthritis (rea) in humans after salmonellosis. human monocytic u937 cells transfected with hla-b27 are less able to eliminate intracellular salmonella enterica serovar enteritidis than those transfected with control hla antigens (e.g. hla-a2). to investigate further the mechanisms by which hla-b27-transfected cells allow increased replication of these bacteria, a dna-based microarray was used for comparativ ...201020670450
dietary fish oil impairs induction of gamma-interferon and delayed-type hypersensitivity during a systemic salmonella enteritidis infection in rats.fish oil that is rich in n-3 polyunsaturated fatty acids markedly modulates immunological responses. literature data indicate that the fish oil reduces cellular immunity and therefore impairs resistance to infections. we have investigated how dietary fish oil affects the immune response against a facultative intracellular pathogen, salmonella enteritidis. wistar rats were fed a diet containing 16% (w/w) of either fish oil or corn oil. after a 4-week adaptation period, rats were intraperitoneally ...201020666739
quantitative studies of the distribution pattern for salmonella enteritidis in the internal organs of chicken after oral challenge by a real-time pcr.this research was undertaken to identify and understand the regular distribution pattern for salmonella enteritidis (s. enteritidis) in the internal organs of chicken after oral challenge over a 3 wk period. we used a real-time, fluorescence-based quantitative polymerase chain reaction (fq-pcr) to detect genomic dna of s. enteritidis in the blood and the internal organs, including heart, liver, spleen, kidney, pancreas, and gallbladder, from chicken after oral challenge at different time points. ...201020665111
contribution of the type vi secretion system encoded in spi-19 to chicken colonization by salmonella enterica serotypes gallinarum and enteritidis.salmonella gallinarum is a pathogen with a host range specific to poultry, while salmonella enteritidis is a broad host range pathogen that colonizes poultry sub-clinically but is a leading cause of gastrointestinal salmonellosis in humans and many other species. despite recent advances in our understanding of the complex interplay between salmonella and their hosts, the molecular basis of host range restriction and unique pathobiology of gallinarum remain largely unknown. type vi secretion syst ...201020661437
protection of epithelial cells from salmonella enterica serovar enteritidis invasion by antibodies against the spi-1 type iii secretion system.salmonella enterica serovar enteritidis (salmonella enteritidis) is one of the major causes of bacterial food-borne illness in humans. during the course of infection, salmonella enteritidis uses 2 type iii secretion systems (t3ss), one of which is encoded on salmonella pathogenicity island 1 (spi-1). spi-1 plays a major role in the invasion process. in the present study, we evaluated the effect of sera against the spi-1 t3ss components on invasion in vitro using polarized human intestinal epithe ...201020657623
a multipathogen selective enrichment broth for simultaneous growth of salmonella enterica serovar enteritidis, staphylococcus aureus, and listeria monocytogenes.a selective enrichment broth (ssl) was formulated to allow concurrent growth of 3 prominent food-borne pathogens: salmonella enterica serovar enteritidis, staphylococcus aureus, and listeria monocytogenes. nalidixic acid, lithium chloride, and potassium tellurite were added as the selective agents, while sodium pyruvate and mannitol were employed as the supplemented elements. in the individual growth trial, the target pathogens were capable of growing in ssl to as high as 7-8 log(10) colony-form ...201020651858
antibacterial activity of extracts from plants of central argentina--isolation of an active principle from achyrocline satureioides.the great increase in bacterial infections is fueling interest in the search for antibacterial products of plant origin. extracts obtained from 51 native and naturalized plants from central argentina were therefore evaluated for their in vitro inhibitory activity on pathogenic bacteria with the aim of selecting the most active ones as new sources of effective antibiotics. the susceptibility of reference and clinical strains of enterococcus faecalis, escherichia coli, proteus mirabilis, pseudomon ...201120645245
comparative antimicrobial susceptibility of biofilm versus planktonic forms of salmonella enterica strains isolated from children with gastroenteritis.in the present study, 194 salmonella enterica strains, isolated from infected children and belonging to various serotypes, were investigated for their ability to form biofilms and the biofilm forms of the isolated strains were compared to their corresponding planktonic forms with respect to the antimicrobial susceptibility. for the biofilm-forming strains, the minimum inhibitory concentration for bacterial regrowth (micbr) from the biofilm of nine clinically applicable antimicrobial agents was d ...201020640867
giant splenic abscess due to salmonella enteritidis in a returning traveler.we report the case of an immunocompetent traveler returning from morocco who presented with a giant splenic abscess, revealing an infection by salmonella enterica serovar enteritidis.201020636602
in vitro penetration of salmonella enteritidis through yolk membranes of eggs from 6 genetically distinct commercial lines of laying hens.although deposition of salmonella enteritidis inside yolks is less common than deposition in albumen or on the vitelline (yolk) membrane in naturally contaminated eggs laid by infected hens, bacterial migration into the yolk to reach its nutrient-rich contents could lead to extensive multiplication. the present study used an in vitro egg contamination model to assess the ability of small initial numbers of salmonella enteritidis to penetrate the vitelline membrane and multiply inside yolks of eg ...201020634530
changes in fourier transform infrared spectra of salmonella enterica serovars typhimurium and enteritidis after adaptation to stressful growth conditions.the effects of growth conditions (temperature in the range 10-45 degrees c, sodium chloride concentration in the range 0-4%, aerobic vs. anaerobic growth and acidification of the growth medium, up to ph 4.5) on the fourier transform infrared (ft-ir) spectra of salmonella enterica serovars typhimurium and enteritidis were studied using multivariate statistical methods (hierarchical cluster analysis and factor analysis). although all environmental factors tested affected s. typhimurium and s. ente ...201020633942
poly(anhydride) nanoparticles act as active th1 adjuvants through toll-like receptor exploitation.the mechanisms that underlie the potent th1-adjuvant capacity of poly(methyl vinyl ether-co-maleic anhydride) nanoparticles (nps) were investigated. traditionally, polymer nps have been considered delivery systems that promote a closer interaction between antigen and antigen-presenting cells (apcs). our results revealed that poly(anhydride) nps also act as agonists of various toll-like receptors (tlrs) (tlr2, -4, and -5), triggering a th1-profile cytokine release (gamma interferon [ifn-gamma], 4 ...201020631332
antimicrobial effects of wine: separating the role of polyphenols, ph, ethanol, and other wine components.while the antimicrobial effectiveness of wine is well documented, relative contributions of the wine components to its antimicrobial activity is controversial. to separate the role of wine phenolics, ethanol, and ph from other wine constituents, the antimicrobial effects of intact wine were compared to that of phenols-stripped wine, dealcoholized wine, ethanol, and low ph applied separately and in combination, against 2 common foodborne pathogens, salmonella enterica serovar enteritidis and esch ...201020629891
emergency endovascular treatment of early spontaneous nonaneurysmal popliteal artery rupture in a patient with salmonella bacteremia.rupture of a nonaneurysmal popliteal artery and subsequent pseudoaneurysm formation is an exceedingly rare event after bacteremia caused by salmonella spp. only a few cases have been reported in the literature. moreover, spontaneous popliteal artery rupture resulting from this pathology, to our knowledge, has not been reported. we describe an early spontaneous rupture of the popliteal artery complicated by acute compartment syndrome in a 67-year-old man who had recently experienced fever, chills ...201020620008
simple and rapid detection of salmonella serovar enteritidis under field conditions by loop-mediated isothermal amplification.the objective of this study is to develop a serovar-specific loop-mediated isothermal amplification (lamp) method for sensitive, rapid, and inexpensive detection of salmonella serovar enteritidis under field conditions.201020618889
reduction of salmonella on inoculated almonds exposed to hot oil.the heat resistance of salmonella inoculated onto almonds was determined after immersion in hot oil. whole almonds were inoculated with salmonella enteritidis pt 30 or salmonella senftenberg 775w and heated in oil. after heating, almonds were drained, transferred to cold tryptic soy broth, and mixed with a stomacher, and samples were plated onto tryptic soy and bismuth sulfite agars. salmonella survivor inactivation curves were upwardly concave. rapid reductions of 2.9, 3.0, or 3.6 log cfu/g for ...201020615336
colonization of avian reproductive-tract tissues by variant subpopulations of salmonella enteritidis.leghorn hens were infected with salmonella enteritidis cultures of known genomic content and subpopulation characteristics to determine comparative abilities to colonize the avian reproductive tract. group 1 received phage-type (pt)4 22079, which is a dimorphic subpopulation that can both contaminate eggs and form biofilm. group 2 received a 90:10 mixture of monomorphic pt13a strains 21027 and 21046, which produce biofilm or contaminate eggs, respectively. group 3 received a 10:90 mixture of the ...201020608530
antimicrobial and antioxidant activities of turkish extra virgin olive oils.turkish extra virgin olive oils (evoo) from different varieties/geographical origins and their phenolic compounds were investigated in terms of their antimicrobial and antioxidant properties in comparison to refined olive, hazelnut, and canola oils. antimicrobial activity was tested against three foodborne pathogenic bacteria, escherichia coli o157:h7, listeria monocytogenes , and salmonella enteritidis. although all evoos showed a bactericidal effect, the individual phenolic compounds demonstra ...201020604567
antibacterial effect (in vitro) of moringa oleifera and annona muricata against gram positive and gram negative bacteria.antibacterial effects of aqueous and ethanolic extracts of seeds of moringa (moringa oleifera) and pods of soursop (annona muricata) in the concentration of 1:5 and 1:10 in volumes 50, 100, 150 and 200 microl were examined against staphylococcus aureus, vibrio cholerae, escherichia coli (isolated from the organism and the aquatic environment) and salmonella enteritidis. antibacterial activity (inhibition halo > 13 mm) against s. aureus, v. cholerae and e. coli isolated from the whiteleg shrimp, ...201020602021
microbial contamination of raw meat and its environment in retail shops in karachi, pakistan.this study was conducted to examine the frequency of contamination in retail meat available in karachi, pakistan.201020601790
salmonella typhimurium and salmonella enteritidis in england: costs to patients, their families, and primary and community health services of the nhs.this is the first study comparing societal costs of acute illness with salmonella typhimurium (st) and salmonella enteritidis (se) in the uk. it included the cost and severity of the illness and explored the impact of each salmonella serovar on the patients, their families, the nhs, and the wider economy. the study ascertained confirmed cases of st and se between july and november 2008. the mean costs per case were £1282 (st) and £993 (se). the indirect costs associated with the work-time lost b ...201120598211
[pyomyositis due to salmonella enteritidis in an immunocompetent patient]. 201020591756
inactivation of campylobacter jejuni by exposure to high-intensity 405-nm visible light.although considerable research has been carried out on a range of environmental factors that impact on the survival of campylobacter jejuni, there is limited information on the effects of violet/blue light on this pathogen. this investigation was carried out to determine the effects of high-intensity 405-nm light on c. jejuni and to compare this with the effects on two other important gram-negative enteric pathogens, salmonella enteritidis and escherichia coli o157:h7. high-intensity 405-nm ligh ...201020590423
salmonella enterica enteritidis arthritis following trauma in a child with thalassemia major.osteoarticular infections caused by non-typhi salmonella are exceptionally encountered. we report a case of a bacteriologically documented knee joint infection due to salmonella enterica serotype enteritidis, following trauma in a child with thalassemia major. emergency arthrotomy combined with antimicrobial therapy was helpful in eradication of infection. physicians should be aware of this rare manifestation of non-typhi salmonella infections in thalassemic patients.201020589479
inhibitory effects of hinokitiol on tyrosinase activity and melanin biosynthesis and its antimicrobial activities.the inhibitory effects of hinokitiol, a constituent of the woody oils isolated from cupressaceae heartwood, on mushroom tyrosinase and melanin formation in b16 melanoma cells as well as its antimicrobial activity were investigated. our results showed that hinokitiol could strongly inhibit both monophenolase activity and diphenolase activity of the enzyme and the inhibition was reversible. the ic(50) values were estimated as 9.67 μm for monophenolase activity and 0.21 μm for diphenolase activity. ...201020578978
salmonella enteritidis-infected intra-abdominal lymphangioma. 201020575889
lipopolysaccharides directly decrease ca2+ oscillations and the hyperpolarization-activated nonselective cation current if in immortalized hl-1 cardiomyocytes.lipopolysaccharide (lps) has been implicated in sepsis-mediated heart failure and chronic cardiac myopathies. we determined that lps directly and reversibly affects cardiac myocyte function by altering regulation of intracellular ca2+ concentration ([ca2+]i) in immortalized cardiomyocytes, hl-1 cells. [ca2+]i oscillated (<0.4 hz), displaying slow and transient components. lps (1 microg/ml), derived either from escherichia coli or from salmonella enteritidis, reversibly abolished ca2+ oscillation ...201020573997
safety assessment and evaluation of probiotic potential of bacteriocinogenic enterococcus faecium kh 24 strain under in vitro and in vivo conditions.in the present investigation, a previously isolated enterococcus faecium kh 24 strain was evaluated for the presence of virulence determinants (agg, esp, efaafm, gele, cyla, cylb, clym, cpd, cob, ccf, ace and hyl), sensitivity to various antibiotics and production of biogenic amines. no virulence determinants were detected, except efaafm. kh 24 was found to be sensitive to most of the tested antibiotics and none of the biogenic amines were produced by it. moreover, kh 24 showed good in vitro tol ...201020570005
transcriptomic responses of salmonella enterica serovars enteritidis and typhimurium to chlorine-based oxidative stress.salmonella enterica serovars enteritidis and typhimurium are the leading causative agents of salmonellosis in the united states. s. enteritidis is predominantly associated with contamination of shell eggs and egg products, whereas s. typhimurium is frequently linked to tainted poultry meats, fresh produce, and recently, peanut-based products. chlorine is an oxidative disinfectant commonly used in the food industry to sanitize the surfaces of foods and food processing facilities (e.g., shell eggs ...201020562293
deletion of a prophage-like element causes attenuation of salmonella enterica serovar enteritidis and promotes protective immunity.salmonella enterica serovar enteritidis (s. enteritidis) is a wide host range serovar belonging to the s. enterica genus. worldwide, it is one of the most frequent causes of food borne disease. similar to s. typhimurium, some virulence genes of s. enteritidis are located in pathogenicity islands and prophages. in this study we have generated a mutant strain of s. enteritidis lacking a prophage-like element, denominated varphise12. the resulting mutant strain was attenuated and promoted protectiv ...201020558245
antimicrobial properties of prunin, a citric flavanone glucoside, and its prunin 6″-o-lauroyl ester.to determine the antimicrobial potential of prunin (p), a flavanone glucoside resulting from the hydrolysis of naringin present in grapefruit, and of its prunin 6″-o-lauroyl ester (pl), synthesized by enzymatic catalysis.201020553344
reactive arthritis and other musculoskeletal sequelae following an outbreak of salmonella hadar in castellon, spain.in 2005 a large outbreak of salmonella hadar occurred in spain following the consumption of commercial precooked roast chicken. we estimated the incidence and risk factors for reactive arthritis (rea) and other musculoskeletal sequelae in the patients of this outbreak in 2 health departments of castellon province.201020551098
antimicrobial effectiveness of oregano and sage essential oils incorporated into whey protein films or cellulose-based filter paper.in this study the antimicrobial effectiveness of oregano and sage essential oils (eos) incorporated into two different matrices, whey protein isolate (wpi) and cellulose-based filter paper, was analysed.201020549805
antimicrobial resistance and subtyping of salmonella enterica subspecies enterica serovar enteritidis isolated from human outbreaks and poultry in southern brazil.to investigate antimicrobial resistance, 96 salmonella enterica subspecies enterica serovar enteritidis strains isolated from salmonellosis outbreaks and poultry-related products obtained in southern brazil were analyzed. macrorestriction patterns, obtained by pulsed-field gel electrophoresis and phage types, were assessed. although 43.75% of samples were sensitive to all drugs tested, resistance to sulfonamide (34.37%), trimethoprim-sulfamethoxazole (25.00%), nalidixic acid (14.58%), streptomyc ...201020548083
incidence of salmonella enteritidis in chicken layer flocks in turkey: results by real-time polymerase chain reaction and international organization for standardization culture methods.this study presents salmonella enteritidis incidence in chicken layer flocks in turkey determined by real-time pcr (rpcr) and by international organization for standardization (iso) method 6579:2002/amd 1:2007. a total of 259 samples, composed of 1,036 individual samples each pooled into 4, including 175 cloacal swab, 14 intestine, 35 gizzard swab, and 35 cecal swab samples, belonging to 6 major companies, were collected from 50 layer flocks and tested by rpcr and iso culture methods. overall in ...201020548068
evaluation of recombinant salmonella expressing cd154 for persistence and enhanced antibody response in commercial turkeys.foodborne illness due to salmonella is a worldwide public health concern and epidemiological evidence has identified poultry and poultry products as a significant source of human salmonella infection. to discover an effective vaccine that protects poultry against multiple salmonella serotypes, several novel attenuated salmonella enteritidis strains (deltase) were developed to express variations of a potential immune-enhancing cd154 peptide sequence on the outer membrane protein lamb in associati ...201020548067
hatchery hygiene evaluation by microbiological examination of hatchery samples.this study was conducted to investigate the bacterial contamination of air and the surface of equipment and facilities in hatchery. in addition, the inhibitory effects of formaldehyde application methods on aerosol bacterial counts in the hatchers were also investigated. in the operating hatchers, the contamination of air by aerobic bacteria, coliform, and fungi was high, measuring over 300 cfu/63.6 cm(2). in the egg sorting room, contamination was moderate, whereas in the remaining sampling sit ...201020548066
isolation and characterization of a novel analyte from bacillus subtilis sc-8 antagonistic to bacillus cereus.in this study, an effective substance was isolated from bacillus subtilis sc-8, which was obtained from traditionally fermented soybean paste, cheonggukjang. the substance was purified by hplc, and its properties were analyzed. it had an adequate antagonistic effect on bacilluscereus, and its spectrum of activity was narrow. when tested on several gram-negative and gram-positive foodborne pathogenic bacteria such as salmonella enterica, salmonella enteritidis, staphylococcus aureus, and listeria ...201020547349
effects of salmonella on spatial-temporal processes of jejunal development in chickens.to study effects of salmonella enteritidis on morphological and functional changes in chicken jejunal development, we analysed gene expression profiles at seven points post-infection in 1-21 day-old broiler chickens. nine clusters with different gene expression patterns were identified, and the genes in each cluster were further analyzed by a functional annotation clustering method (david). functional and morphological developmental processes dominated in all the nine clusters. salmonella infect ...201020541565
survival of salmonella and staphylococcus aureus in mexican red salsa in a food service setting.mexican red salsa is one of the most common side dishes in mexican cuisine. according to data on foodborne illnesses collected by the centers for disease control and prevention, salsa was associated with 70 foodborne illness outbreaks between 1990 and 2006. salsa ingredients such as tomatoes, cilantro, and onions often have been implicated in foodborne illness outbreaks. mexican-style restaurants commonly prepare a large batch of red salsa, store it at refrigeration temperatures, and then serve ...201020537270
antimicrobial activity of basil (ocimum basilicum) oil against salmonella enteritidis in vitro and in food.nine essential oils were examined for antimicrobial activity against reference and clinical strains of salmonella enteritidis. based on the size of the inhibition zone and the minimal inhibitory concentration, basil oil had the strongest antimicrobial activity against all the tested bacteria, and s. enteritidis se3 was the most sensitive strain to all the tested oils. gas chromatography/mass spectrometry analysis revealed that the major constituents of the oil were linalool (64.35%), 1,8-cineole ...201020530897
mass spectrometry-based quantitative proteomic analysis of salmonella enterica serovar enteritidis protein expression upon exposure to hydrogen peroxide.salmonella enterica, a common food-borne bacterial pathogen, is believed to change its protein expression profile in the presence of different environmental stress such as that caused by the exposure to hydrogen peroxide (h2o2), which can be generated by phagocytes during infection and represents an important antibacterial mechanism of host cells. among salmonella proteins, the effectors of salmonella pathogenicity island 1 and 2 (spi-1 and spi-2) are of particular interest since they are expres ...201020529336
successful treatment of late salmonella infections in total hip replacement - report of two cases.salmonella species can be rarely isolated from periprosthetic joint infections, however when present, are usually part of a severe septic clinical picture.201020529326
expression of chicken leap-2 in the reproductive organs and embryos and in response to salmonella enterica infection.in recent years host antimicrobial peptides and proteins have been recognised as key mediators of the innate immune response in many vertebrate species, providing the first line of defense against potential pathogens. in chickens a number of cationic antimicrobial peptides have been recently identified. however, although these peptides have been studied extensively in the avian gastrointestinal tract, little is known about their function in the chicken reproductive organs and embryos. chicken li ...201020526889
plasmid fingerprinting and virulence gene detection among indigenous strains of salmonella enterica serovar enteritidis.salmonella enterica serovar enteritidis is an important frequently reported zoonotic pathogen and a common cause of human gastroenteritis worldwide. the highly conserved sero-specific plasmids (ssps) and salmonella plasmid virulence (spy) genes have been shown to mediate extra-intestinal colonization and systemic infection. the objective of current study was to document the presence of ssps and spvb/spvc genes prevailing in the indigenous population of serovar enteritidis.200920524477
poly(anhydride) nanoparticles as adjuvants for mucosal vaccination.in the last years, many efforts have been directed toward the enhancement of vaccine delivery by using polymeric nanoparticles as adjuvants for mucosal immunization. however, conventional nanoparticles usually display a low capability to target specific sites within the gut and, thus, the elicited immune responses are not as high as necessary to offer the adequate protection to the host. to overcome these drawbacks, one possible strategy can be the association of nanoparticles with compounds inv ...201020515831
henoch-schönlein nephritis triggered by salmonella enteritidis infection. 201020514113
the interaction between prior antimicrobial drug exposure and resistance in human salmonella infections.the use of antimicrobial drugs for food animals selects for resistant non-typhoid salmonella strains, but human consumption of antimicrobial drugs may also increase the risk of subsequent infection. the aim of this study was to determine the risk of salmonellosis attributable to human consumption of antimicrobial drugs in a case-control study of 22 602 laboratory-confirmed salmonella infections, diagnosed in denmark between 1997 and 2005.201020507862
antiviral and anti-inflammatory diterpenoids from the soft coral sinularia gyrosa.chemical investigation of the soft coral sinularia gyrosa led to the purification of three new diterpenoids, designated as gyrosanols a-c (1-3). the structures of 1-3 were elucidated through extensive spectroscopic analyses. compounds 1 and 2 exhibited antiviral activity against hcmv with ic(50)'s of 2.6 and 3.7 microm, respectively. in addition, compounds 1 and 2 showed significant anti-inflammatory activity by reducing the levels of the cox-2 protein (19.6 + or - 3.9% and 29.1 + or - 9.6%, res ...201020499851
chicken tlr21 is an innate cpg dna receptor distinct from mammalian tlr9.tlrs comprise a family of evolutionary conserved sensory receptors that respond to distinct classes of ligands. for one major evolutionary branch of tlrs, the ligands are still largely unknown. here we report the cloning and function of one member of this group, chicken tlr21 (chtlr21). this tlr is absent in the human species but has homologs in fish and frog and displays similarity with mouse tlr13. expression of chtlr21 in hek293 cells resulted in activation of nf-kappab in response to unmethy ...201020498358
salmonella enterica serovar enteritidis tatb and tatc mutants are impaired in caco-2 cell invasion in vitro and show reduced systemic spread in chickens.salmonella enterica subsp. enterica serovar enteritidis is a leading causative agent of gastroenteritis in humans. this pathogen also colonizes the intestinal tracts of poultry and can spread systemically in chickens. transfer to humans usually occurs through undercooked or improperly handled poultry meat or eggs. the bacterial twin-arginine transport (tat) pathway is responsible for the translocation of folded proteins across the cytoplasmic membrane. in order to study the role of the tat syste ...201020498258
ema-real-time pcr as a reliable method for detection of viable salmonella in chicken and eggs.culture-based salmonella detection takes at least 4 d to complete. the use of taqman probes allows the real-time pcr technique to be a rapid and sensitive way to detect foodborne pathogens. however, unlike rna-based pcr, dna-based pcr techniques cannot differentiate between dna from live and dead cells. ethidium bromide monoazide (ema) is a dye that can bind to dna of dead cells and prevent its amplification by pcr. an ema staining step prior to pcr allows for the effective inhibition of false p ...201020492302
efficacy of aqueous and alcohol-based quaternary ammonium sanitizers for reducing salmonella in dusts generated in almond hulling and shelling facilities.large volumes of fine particulate matter or "dust" (soil, hulls, and shells) generated when hulls and shells are removed from almond kernels complicate cleaning and sanitation procedures in the huller-sheller (hs) environment. this study evaluated the efficacy of 3 aqueous quaternary ammonium sanitizers (aquats) and an isopropyl alcohol-based quaternary ammonium sanitizer (ipaquat) for reducing salmonella in dust collected from 2 hs facilities. dust (1 g) was thoroughly mixed with 1 to 2 ml of i ...201020492187
thermal inactivation and postthermal treatment growth during storage of multiple salmonella serotypes in ground beef as affected by sodium lactate and oregano oil.we assessed the heat resistance of salmonella in raw ground beef in both the absence and presence of sodium lactate, oregano oil, and in combinations of these 2 gras-listed ingredients, and determined their bactericidal or bacteriostatic activities during postthermal treatment storage at 15 degrees c. a cocktail of 8 serotypes of salmonella spp. was inoculated into ground beef supplemented with sodium lactate (nal) (1.5% and 3%) and/or oregano oil (0.5% and 1%) to obtain approximately 8 log cfu/ ...201020492179
salmonella enterica subspecies enterica serovar enteritidis salmonella pathogenicity island 2 type iii secretion system: role in intestinal colonization of chickens and systemic spread.salmonella enterica subspecies enterica serovar enteritidis (s. enteritidis) has been identified as a significant cause of salmonellosis in humans. salmonella pathogenicity islands 1 and 2 (spi-1 and spi-2) each encode a specialized type iii secretion system (t3ss) that enables salmonella to manipulate host cells at various stages of the invasion/infection process. for the purposes of our studies we used a chicken isolate of s. enteritidis (sal18). in one study, we orally co-challenged 35-day-ol ...201020488876
prevalence of salmonella enterica in poultry and eggs in uruguay during an epidemic due to salmonella enterica serovar enteritidis.salmonella enterica serovar enteritidis (s. enteritidis) is frequently associated with food-borne disease worldwide. poultry-derived products are a major source. an epidemic of human infection with s. enteritidis occurred in uruguay, and to evaluate the extent of poultry contamination, we conducted a nationwide survey over 2 years that included the analysis of sera from 5,751 birds and 12,400 eggs. serological evidence of infection with salmonella group o:9 was found in 24.4% of the birds. all p ...201020484605
early host gene expression responses to a salmonella infection in the intestine of chickens with different genetic background examined with cdna and oligonucleotide microarrays.so far the responses of chickens to salmonella have not been studied in vivo on a whole genome-wide scale. furthermore, the influence of the host genetic background on gene expression responses is unknown. in this study gene expression profiles in the chicken (gallus gallus) intestine of two genetically different chicken lines were compared, 24 h after a salmonella enteritidis inoculation in 1-day-old chicks. the two chicken lines differed in the severity of the systemic infection. for gene expr ...200620483261
evaluation of iso 6579 and fda-bam methods to complement real-time polymerase chain reaction for the detection of salmonella in naturally contaminated poultry meat and red meat.in this study, we evaluated the salmonella detection capability and compatibility of a lightcycler polymerase chain reaction (lc pcr) system with two bacteriological methods, united states food and drug administration's bacteriological analytical manual chapter 5: salmonella (fda) and international organization for standardization method 6579 (iso). the aim was to determine which bacteriological method would support lc pcr for testing naturally contaminated poultry and red meat samples with salm ...201020482231
[primary retrofascial abscess in the iliopsoas muscle caused by salmonella enteritidis.].this case report and review of literature demonstrates a rare entity of a primary retrofascial abscess in the iliopsoas muscle caused by salmonella enteritidis, that progressed into the iliac fossa. it is of importance to the orthopedic surgeon in his differential diagnosis of hip pain and vertebrogenic disorders. it can also resemble other infectious diseases or tumors as it occurs mainly in young or imunosuppressed individuals. the primary and secondary etiology of this disease and it's differ ...200020478198
the effect of long-term propionate adaptation on the stress resistance of salmonella enteritidis.adaptive phenotypes of enteric bacterial pathogens in response to in vivo-mimicking stress conditions are important because of their potentiality to enhance stress resistance and ameliorate measures intended to control transmission and infectivity. salmonella enterica serotype enteritidis (s. enteritidis) encounters a variety of such environments throughout its infection cycle, including high concentrations of the short-chain fatty acid, propionate (pa), during food processing and within the gut ...201020477896
suppurative cholecystitis with cholelithiasis in a human "carrier" of the bacillus enteritidis of gaertner. 191120474453
the toxin of the bacillus enteritidis of gärtner. 190620474254
[salmonella and campylobacter infections in 2008].information about whether salmonella and campylobacter infections are acquired during foreign travel has previously been incomplete. we collected such information systematically for 2008.201020470656
hot air treatment for surface decontamination of table eggs experimentally infected with salmonella, listeria, and escherichia coli.hot-air pasteurization was investigated in the eu-funded project "reducing egg susceptibility to contaminations in avian production in europe (rescape)" as an innovative treatment for surface bacterial decontamination of table eggs. possible side effects of the treatment on egg quality traits were also studied. the decontamination power of hot air was evaluated over 1 month on shell eggs that were experimentally inoculated with salmonella enteritidis, escherichia coli, or listeria monocytogenes. ...201020461457
evaluation of pcr inhibitory effect of enrichment broths and comparison of dna extraction methods for detection of salmonella enteritidis using real-time pcr assay.the best enrichment broth and dna extraction scheme was determined for rapid and sensitive detection of salmonella enteritidis in steamed pork using real-time pcr. the inhibitory effect of commonly used salmonella enrichment broths, rappaport-vassiliadis (rv) and muller-kauffmann tetrathionate with novobiocin (mkttn), on real-time pcr was confirmed. the inhibition of pcr was statistically significant (p < 0.05) in rv and mkttn, as compared with buffered peptone water (bpw) or phosphate-buffered ...201020458155
[a boy with a red swelling of the lower leg].a 13-year-old boy presented with erythema nodosum on the anterior and lateral side of his lower legs due to enteritis caused by salmonella enteritidis.201020456800
salmonella enteritidis infections associated with a contaminated immersion blender at a camp.more than 100 foodborne salmonellosis outbreaks occur each year in the united states. contaminated food preparation equipment is implicated in approximately 32% of salmonella outbreaks with a known source. in april 2009, we investigated reported salmonella infections at a camp in new hampshire. camp attendees were contacted to complete a standard questionnaire. the questionnaire asked about foods eaten while at the camp, symptoms of gastrointestinal illness, visits to healthcare providers, and s ...201020455756
investigation of risk factors for salmonella on commercial egg-laying farms in great britain, 2004-2005.in 2004/05, all european union member states were required to carry out standardised prevalence surveys to establish the baseline prevalence of salmonella in commercial laying flocks. as part of the survey in great britain, additional data were collected from 380 of the enrolled laying hen holdings to investigate risk factors for salmonella at farm level. stratified, simple random sampling was used to select holdings from which dust and boot swab samples were collected and tested for salmonella ...201020453235
multidrug-resistant salmonella isolates from retail chicken meat compared with human clinical isolates.to examine the prevalence of antimicrobial-resistant salmonella in chicken meat and correlate with isolates from ill humans.201020443729
the role of bioactive substances in controlling foodborne pathogens derived from metasequoia glyptostroboides miki ex hu.in an attempt to isolate bioactive substances, ethyl acetate cone extract of metasequoia glyptostroboides was subjected to a column chromatographic analysis that resulted in isolation of an abietane type diterpenoid, taxoquinone. its structure was elucidated by spectroscopic means. in further, taxoquinone showed potential antibacterial effect as diameters of zones of inhibition against foodborne pathogenic bacteria such as listeria monocytogenes atcc 19166, salmonella typhimurium kctc 2515, salm ...201020435080
preliminary study on the efficacy and safety of eight individual and blended disinfectants against poultry and dairy indicator organisms.eight individual and blended chemical disinfectants were screened for preliminary evaluation of safety, bactericidal and virucidal effectiveness against poultry and dairy organisms. the test organisms were escherichia coli, salmonella enteritidis, staphylococcus aureus, streptococcus spp. and clostridium perfringens, in addition to avian influenza virus (aiv) and newcastle disease virus (ndv). viable counts of surviving bacteria were determined after 30 min contact with each disinfectant and in ...200720422561
outbreak of salmonella enteritidis b in a family in southern taiwan.this study describes a salmonella outbreak in a 9-member family in southern taiwan, and emphasizes the risk of salmonella infection in extreme age.200920422139
application of chitosan-incorporated ldpe film to sliced fresh red meats for shelf life extension.chitosan lactate was impregnated as an antimicrobial additive into low density polyethylene (ldpe) with different concentrations. the antimicrobial effectiveness was tested with three pathogenic bacteria, specifically listeria monocytogenes, escherichia coli and salmonella enteritidis. also, these chitosan incorporated films were applied on red meat surfaces to determine the effectiveness of chitosan on color shelf life extension and microbial growth inhibition. chitosan was exposed to 0.1% pept ...201020416821
safety and quality of ready-to-eat dry fermented sausages subjected to e-beam radiation.the inactivation kinetics in the death of listeriainnocua ntc 11288 (more radioresistant than five different strains of listeriamonocytogenes) and salmonella enterica serovar enteritidis and s. enterica serovar typhimurium by e-beam irradiation has been studied in two types of vacuum-packed rte dry fermented sausages ("salchichon" and "chorizo") in order to optimize the sanitation treatment of these products. a treatment of 1.29kgy was calculated to reach the food safety objective (fso) accordin ...200920416723
evaluation of the effect of defrosting practices of ground beef on the heat tolerance of listeria monocytogenes and salmonella enteritidis.the effect of common defrosting practices of ground beef, including (i) defrosting in the refrigerator (5 degrees c for 15h), (ii) defrosting at room temperature (25 degrees c for 12h) and (iii) defrosting in the microwave, on the heat tolerance of artificially inoculated listeria monocytogenes and salmonella enteritidis, was studied. the thermal inactivation of s. enteritidis was not, overall, affected by defrosting practices. in contrast, defrosting at room temperature resulted, overall, in an ...200920416679
erythema nodosum associated with salmonella enteritidis.erythema nodosum (en) is the most frequent type of panniculitis in childhood. although frequently idiopathic, it may be associated with a wide variety of conditions ranging from infections, to sarcoidosis, to collagen vascular diseases to drugs.201020411062
isolation of salmonella enterica serovar enteritidis in blue-fronted amazon parrot (amazona aestiva).avian salmonellosis is a disease caused by bacteria of the genus salmonella that can cause three distinct diseases in birds: pullorum diseases, fowl typhoid, and paratyphoid infection. various wildlife species are susceptible to infections by salmonella, regardless of whether they live in captivity or freely in the wild. the present study verified the presence of salmonella enterica serovar enteritidis in three captive specimens of amazona aestiva. the study involved a total of 103 birds undergo ...201020408416
drug resistance, plasmid profile and random amplified polymorphic dna analysis of iranian isolates of salmonella enteritidis.the aim of this study was to characterize 49 salmonella enteritidis (se) isolates from different sources (poultry, human, cow, poultry house environment) in iran with respect to drug resistance, plasmid profile, and random amplified polymorphic dna (rapd) analysis. antimicrobial susceptibility test to 29 agents found 33 resistance patterns among the isolates. no resistance was observed to danofloxacin, levofloxacin, norfloxacin, ceftriaxone, imipenem, and amikacin. the highest resistance (38.8%) ...201020402413
evaluation of efflux activity of bacteria by a semi-automated fluorometric system.a semi-automated method that uses the common efflux pump (ep) substrate ethidium bromide (eb) is described for the assessment of ep systems of bacteria. the method employs the rotor-gene(tm) 3000 thermocycler (corbett research) for the real-time assessment of accumulation and efflux of eb in phosphate-buffered solution (pbs) under varying physiological conditions, such as temperature, ph, presence and absence of the energy source, and presence of efflux pumps inhibitors (epis). the method is suf ...201020401593
characterization of antimicrobial resistant salmonella enterica serovars enteritidis and typhimurium isolates from animal and food in southern italy.in the last two decades, the emergence and spread of antimicrobial-resistant pathogens, among them salmonella, has become a serious health hazard worldwide, and specifically the high incidence of multidrug resistance has been encountered widely in many european countries. this study examines the antimicrobial susceptibility of salmonella enterica strains typhimurium and enteritidis isolated in campania and calabria region (southern italy) from animal and food of animal origin. the relationship o ...201020401533
rck of salmonella enterica, subspecies enterica serovar enteritidis, mediates zipper-like internalization.salmonella can invade non-phagocytic cells through its type iii secretion system (t3ss-1), which induces a trigger entry process. this study showed that salmonella enterica, subspecies enterica serovar enteritidis can also invade cells via the rck outer membrane protein. rck was necessary and sufficient to enable non-invasive e. coli and rck-coated beads to adhere to and invade different cells. internalization analysis of latex beads coated with different rck peptides showed that the peptide con ...201020368731
molecular epidemiology of ampicillin resistance in salmonella spp. and escherichia coli from wastewater and clinical specimens.molecular epidemiology at local scale in sicily (italy) of ampicillin resistance in salmonella spp. isolates from municipal wastewater (n = 64) and clinical specimens (n = 274) is described in comparison with previously examined escherichia coli isolates (n = 273) from wastewater. high prevalence of antibiotic resistance (28.9%) with highest resistance rates against ampicillin (22.7%) was observed in e. coli isolates. different resistance rates were observed in salmonella according to the serova ...201020367333
effectiveness of superheated steam and gas catalytic infrared heat treatments to inactivate salmonella on raw almonds.the majority of the almond-related outbreaks have been associated with salmonella. therefore, it is necessary to find an effective method to inactivate these organisms on the raw almond before distribution in the market. this study was conducted to assess the effectiveness of superheated steam (shs) treatments followed by catalytic infrared (ir) heat treatment to inactivate salmonella populations on raw almond and to determine the effect of these treatments on the quality of raw almond. it has b ...201020367332
comparison of genotypes of salmonella enterica serovar enteritidis phage type 30 and 9c strains isolated during three outbreaks associated with raw almonds.in 2000 to 2001, 2003 to 2004, and 2005 to 2006, three outbreaks of salmonella enterica serovar enteritidis were linked with the consumption of raw almonds. the s. enteritidis strains from these outbreaks had rare phage types (pt), pt30 and pt9c. clinical and environmental s. enteritidis strains were subjected to pulsed-field gel electrophoresis (pfge), multilocus variable-number tandem repeat analysis (mlva), and dna microarray-based comparative genomic indexing (cgi) to evaluate their genetic ...201020363782
application of fourier transform infrared spectroscopy and chemometrics for differentiation of salmonella enterica serovar enteritidis phage types.fourier transform infrared (ft-ir) spectroscopy and chemometric techniques were used to discriminate five closely related salmonella enterica serotype enteritidis phage types, phage type 1 (pt1), pt1b, pt4b, pt6, and pt6a. intact cells and outer membrane protein (omp) extracts from bacterial cell membranes were subjected to ft-ir analysis in transmittance mode. spectra were collected over a wavenumber range from 4,000 to 600 cm(-1). partial least-squares discriminant analysis (pls-da) was used t ...201020363777
isolation and identification of the antibacterial active compound from petroleum ether extract of neem oil.from a petroleum ether extract of neem oil (azadirachta indica a. juss) the new tetrahydrofuranyl diester 1 was isolated as an anti-bacterial constituent. 1 showed significant activities against three standard bacterial strains, including staphylococcus aureus atcc 25923, escherichia coli atcc 25922 and salmonella enteritidis cmcc (b) 50041.201020362038
genomic and proteomic evaluation of antibiotic resistance in salmonella strains.using salmonella strains identical to those present in the gastrointestinal tract of different animals we aim to determine and compare the proteome of two serotypes, salmonella typhimurium and enteritidis recovered from faecal samples of wild boars and wild rabbits, respectively. the presence of genes responsible for antibiotic resistance was detected by pcr. proteomes of the two distinct serotypes were determined using 2-de in order to identify proteins associated with antibiotic resistance or ...201020346428
[literature survey of salmonella contamination in eggs and egg products in the world].salmonella species are common bacterial pathogens associated with human gastroenteritis worldwide. in japan, salmonellosis is one of the main food-borne bacterial illnesses and, especially, salmonella enteritidis infections have been strongly associated with the consumption of eggs and egg-containing foods. in this study, we performed the literature survey of salmonella contamination in shell eggs and liquid eggs worldwide for comparing the prevalence among the countries and summarized in the ta ...200920306712
evaluation of suspension array analysis for detection of egg yolk antibodies against salmonella enteritidis.salmonella enterica serovar enteritidis (se) is an important source of food-related diarrhoea in humans, and table eggs are considered the primordial source of contamination of the human food chain. using eggs collected at egg-packing stations as samples could be a convenient strategy to detect colonization of layer flocks. the aim of this study was to evaluate egg yolk anti-salmonella antibody detection using suspension array analysis. an egg yolk panel from contact-infected and non-colonized l ...201020303192
expression of the avian-specific toll-like receptor 15 in chicken heterophils is mediated by gram-negative and gram-positive bacteria, but not tlr agonists.toll-like receptors (tlrs) are a critical component of the innate immune response of mammalian and avian species. while most mammalian tlrs have been well characterized, the chicken-specific tlr15 has not been extensively studied. we recently demonstrated that tlr15 is differentially expressed between salmonella-susceptible-and-resistant chickens, indicating a potential role in the innate immune response to infection with salmonella. the aim of the present study was to gain better insight into t ...201020303182
the high-adhesive properties of the fimh adhesin of salmonella enterica serovar enteritidis are determined by a single f118s substitution.the binding properties of low- and high-adhesive forms of fimh adhesins from salmonella enterica serovars enteritidis and typhimurium (s. enteritidis and s. typhimurium) were studied using chimeric proteins containing an additional peptide that represents an n-terminal extension of the fimf protein. this modification, by taking advantage of a donor strand exchange mechanism, closes the hydrophobic groove in the fimbrial domain of the fimh adhesin. such self-complemented adhesins (scfimh) did not ...201020299404
salmonella enteritidis var. dublin infection in a cow resulting in an outbreak of human gastro-enteritis. 194720295964
septicemia due to salmonella enteritidis. 194720254160
salmonella interitidis infection associated with use of anti-rodent virus. 194720241156
studies on fevers due to paratyphoid organisms and bact. enteritidis. 194720241019
identification by pcr of non-typhoidal salmonella enterica serovars associated with invasive infections among febrile patients in mali.in sub-saharan africa, non-typhoidal salmonella (nts) are emerging as a prominent cause of invasive disease (bacteremia and focal infections such as meningitis) in infants and young children. importantly, including data from mali, three serovars, salmonella enterica serovar typhimurium, salmonella enteritidis and salmonella dublin, account for the majority of non-typhoidal salmonella isolated from these patients.201020231882
inactivation of salmonella enteritidis strains by combination of high hydrostatic pressure and nisin.the effects of high hydrostatic pressure (hhp) and nisin treatment alone and in combination on cellular components and viability of two salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) strains were evaluated by differential scanning calorimetry (dsc) and plate counting in order to evaluate the relative resistance and optimize the treatment conditions. s. enteritidis fda and osu 799 strains were subjected to hhp (0.1-550 mpa for 10 min at 25 degrees c) alone and in combina ...201020226554
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