Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| major outer membrane protein omp25 of brucella suis is involved in inhibition of tumor necrosis factor alpha production during infection of human macrophages. | brucella spp. can establish themselves and cause disease in humans and animals. the mechanisms by which brucella spp. evade the antibacterial defenses of their host, however, remain largely unknown. we have previously reported that live brucellae failed to induce tumor necrosis factor alpha (tnf-alpha) production upon human macrophage infection. this inhibition is associated with a nonidentified protein that is released into culture medium. outer membrane proteins (omps) of gram-negative bacteri ... | 2001 | 11447156 |
| protection of balb/c mice against brucella abortus 544 challenge by vaccination with bacterioferritin or p39 recombinant proteins with cpg oligodeoxynucleotides as adjuvant. | the p39 and the bacterioferrin (bfr) antigens of brucella melitensis 16m were previously identified as t dominant antigens able to induce both delayed-type hypersensivity in sensitized guinea pigs and in vitro gamma interferon (ifn-gamma) production by peripheral blood mononuclear cells from infected cattle. here, we analyzed the potential for these antigens to function as a subunitary vaccine against brucella abortus infection in balb/c mice, and we characterized the humoral and cellular immune ... | 2001 | 11447155 |
| [detection of brucella melitensis by bactec 9050 system]. | to evaluate bactec 9050 system capacity for detection of bacteremia due to brucella spp. | 2001 | 11440664 |
| identification of brucella spp. genes involved in intracellular trafficking. | after uptake by host cells, the pathogen brucella transits through early endosomes, evades phago-lysosome fusion and replicates in a compartment associated with the endoplasmic reticulum (er). the molecular mechanisms underlying these processes are still poorly understood. to identify new bacterial factors involved in these processes, a library of 1800 brucella melitensis 16m mini-tn5catkm mutants was screened for intracellular survival and multiplication in hela cells and j774a.1 macrophages. t ... | 2001 | 11437834 |
| diagnostic value of brucella elisa igg and igm in bacteremic and non-bacteremic patients with brucellosis. | the diagnostic value of brucella elisa igg and igm has been evaluated in patients with brucellosis. serum samples and blood cultures were collected from 83 patients with brucellosis. the sera were tested by brucella elisa for brucella igm and igg antibodies. all 44 controls were negative for igg and igm. brucella melitensis was isolated from blood cultures of 30/83 (36.1%) patients. among the 30 bacteremic patients, 24 (80%) had an increased igm titer of > or = 200. of the 53 non-bacteremic pati ... | 2001 | 11434531 |
| brucellosis control in saudi arabia: prospects and challenges. | brucellosis is a zoonotic disease of worldwide distribution. despite its control in many developed countries the disease remains endemic in saudi arabia where the national seroprevalence of the disease is 15%. in saudi arabia the disease is introduced through uncontrolled importation of animals that are poorly screened for the disease. every year the kingdom imports a few million heads of sheep and goats for sacrifice during hajj from africa, india, and autstralia. brucella melitensis remains th ... | 2001 | 11434523 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 20-2001. a 64-year-old man with fever and gram-negative bacteremia. | 2001 | 11430331 | |
| infective endocarditis in renal transplant recipients. | because of the increasing number of renal transplantations performed and the rarity of reported cases of infective endocarditis in these patients, we studied the clinical characteristics of this infection in this population. we report on two cases from our experience and review reported cases of infective endocarditis in renal transplant recipients retrieved from the medline system. in addition, we reviewed a large series of infective endocarditis looking for patients with renal transplants. in ... | 1999 | 11428982 |
| use of recombinant bp26 protein in serological diagnosis of brucella melitensis infection in sheep. | previously a brucella protein named cp28, bp26, or omp28 has been identified as an immunodominant antigen in infected cattle, sheep, goats, and humans. in the present study we evaluated antibody responses of infected and b. melitensis rev.1-vaccinated sheep to the bp26 protein using purified recombinant bp26 protein produced in escherichia coli in an indirect enzyme-linked immunosorbent assay (i-elisa). the specificity of the i-elisa determined with sera from healthy sheep (n = 106) was 93%. the ... | 2001 | 11427425 |
| re-examination of the role of the brucella melitensis htra stress response protease in virulence in pregnant goats. | based on previously reported studies describing the experimental infection of pregnant goats with b. melitensis strain rwp5, we proposed that the htra protease plays an important role in the virulence of b. melitensis in its natural ruminant host. subsequent studies, however, have shown that rwp5 is actually an htra cycl double mutant. in order to definitively evaluate the role of the b. melitensis htra in virulence, we constructed an authentic htra mutant and examined this strain in pregnant go ... | 2001 | 11423199 |
| successful management of brucella mellitensis endocarditis with combined medical and surgical approach. | brucella endocarditis is an underdiagnosed complication of human brucellosis, associated with high morbidity and mortality. we report the successful management of a number of cases of brucella mellitensis endocarditis. | 2001 | 11404134 |
| deletion of wboa enhances activation of the lectin pathway of complement in brucella abortus and brucella melitensis. | brucella spp. are gram-negative intracellular pathogens that survive and multiply within phagocytic cells of their hosts. smooth organisms present o polysaccharides (ops) on their surface. these ops help the bacteria avoid the bactericidal action of serum. the wboa gene, coding for the enzyme glycosyltransferase, is essential for the synthesis of o chain in brucella. in this study, the sensitivity to serum of smooth, virulent brucella melitensis 16m and b. abortus 2308, rough wboa mutants vtrm1, ... | 2001 | 11401980 |
| intracellular survival of brucella spp. in human monocytes involves conventional uptake but special phagosomes. | brucella spp. are facultative intracellular parasites of various mammals, including humans, typically infecting lymphoid as well as reproductive organs. we have investigated how b. suis and b. melitensis enter human monocytes and in which compartment they survive. peripheral blood monocytes readily internalized nonopsonized brucellae and killed most of them within 12 to 18 h. the presence of brucella-specific antibodies (but not complement) increased the uptake of bacteria without increasing the ... | 2001 | 11349069 |
| identification of brucella abortus, b. canis, b. melitensis, and b. suis by carbon substrate assimilation tests. | by using the results of seven carbon substrate assimilation tests from the biotype 100 system (biomérieux, marcy-l'etoile, france), we correctly identified 79 (85.9%) of 92 brucella strains tested. the specificity of the method varied from 97.4 to 100% depending on the species. although a biological safety cabinet must be used, this method represents an easy and fast alternative for the identification of brucella species. | 2001 | 11348772 |
| severe epistaxis in brucellosis-induced isolated thrombocytopenia: a report of two cases. | brucellosis can present initially with its haematological findings including anaemia, leukopenia, and thrombocytopenia and may mimic primary haematological diseases. we present two patients with complaints of severe epistaxis and isolated thrombocytopenia which was initially diagnosed as idiopathic thrombocytopenic purpura but which was finally attributed to brucellosis. their platelet count reverted to normal within 2-3 weeks of initiating antibrucellosis treatment with recovery from the diseas ... | 2000 | 11318806 |
| limited diagnostic usefulness of antibodies to cytoplasmic proteins of brucella in early-treated human brucellosis. | antibodies to cytoplasmic proteins (cp) of brucella have been shown to be useful for the diagnosis of human brucellosis; however, some early-diagnosed patients lack such an antibody response while having high titers of antibodies to lipopolysaccharide (lps). to address which factors determine this serological discrepancy in the early stages of brucellosis we examined the antibody response to cp and lps of 21 patients involved in an outbreak of b. melitensis infection who had a short duration of ... | 2001 | 11303810 |
| dactylitis in a patient with brucellosis. | dactylitis is an important feature of inflammatory arthritis and unusual complication of osteoarticular brucellosis. we report a case of dactylitis of the index finger in a female patient with brucellosis. | 2001 | 11281675 |
| bacteriologic and histologic features in mice after intranasal inoculation of brucella melitensis. | to characterize effects of intranasal inoculation of virulent brucella melitensis strain 16m in mice. | 2001 | 11277206 |
| current understanding and management of chronic hepatosplenic suppurative brucellosis. | to outline the characteristics and define appropriate management of chronic hepatosplenic suppurative brucellosis (chsb), 905 patients with brucellosis were analyzed. sixteen episodes of chsb (14 in the liver and 2 in the spleen) were found in 15 patients. six patients had had previous remote brucellosis. twelve patients presented with systemic symptoms, and 12 with local symptoms. cultures of blood samples yielded negative results in all cases except 1, and the results of cultures of pus specim ... | 2001 | 11264030 |
| multiplexing for the detection of multiple biowarfare agents shows promise in the field. | standard amplification of nucleic acids, or polymerase chain reaction (pcr), is replacing the more traditional microbiological assays in the detection of biological agents. however, standard pcr is designed as a one program-to-one agent amplification method, and not knowing what agents to test for makes this approach time consuming. during a field training exercise to detect four biowarfare agents using the standard pcr method, we conducted an additional experiment that reduced the diagnostic ti ... | 2001 | 11263027 |
| [failure to detect brucella melitensis in 3 hemoculture systems]. | 2001 | 11256249 | |
| [bacteremia caused by brucella sp. with negative conventional serology]. | 2001 | 11256247 | |
| identification of four genes of the brucella melitensis atp synthase operon f0 sector: relationship with the rhodospirillaceae family. | we have determined the nucleotide sequence of a cloned dna fragment from the human and animal pathogen brucella melitensis. four genes were identified from a 4069 bp fragment, corresponding to the b. melitensis a, c, b', and b subunits of the atp synthase f0 sector operon. a duplicated and divergent copy of the b-subunit gene was observed. this feature has been found only in photosynthetic bacteria and chloroplasts. in addition, the gene cluster was separated from the f1 sector, a characteristic ... | 2000 | 11252353 |
| acute cholecystitis caused by brucella melitensis: case report and review. | brucellosis is a common zoonosis in many parts of the world. human brucellosis is a multisystemic disease with a wide range of clinical symptoms. biliary involvement in the form of acute cholecystitis is a very rare manifestation of brucellosis. here we report the case of a 34-year-old male with acute cholecystitis caused by brucella melitensis and review seven other cases. we conclude that brucella may have a focal effect on the gallbladder, in the form of acute cholecystitis. | 2001 | 11243761 |
| diagnosis: a janeway lesion and an osler's node. | 2001 | 11202109 | |
| brucellosis in laboratory workers at a saudi arabian hospital. | background: saudi arabia is hyperendemic for brucellosis, with more than 8000 cases reported each year to public health authorities. during 1998, brucellosis ranked as the no. 1 reportable communicable disease (22.5%) in saudi arabian national guard communities. king fahad hospital is the major referral center for national guard personnel in the nation's central region. methods and results: from 1991 to 2000, brucellosis developed in 7 expatriate hospital employees. six employees were bacteriolo ... | 2001 | 11172318 |
| therapeutic effects of rifampin and erythromycin in experimental murine brucellosis. | 2000 | 11168083 | |
| real-time detection of brucella abortus, brucella melitensis and brucella suis. | real-time pcr-based assays specific for brucella abortus, brucella melitensis and brucella suis were developed. the assays utilize an upstream primer that is derived from 3' end of the genetic element is 711, whereas the downstream primers and probes are designed from signature sequences specific to a species or a biovar. the pcr reactions were monitored for fluorescence resonance energy transfer by including two adjacent labeled probes that hybridize to the amplicons as they are formed. the ups ... | 2001 | 11162079 |
| use of bactec 9240 blood culture system for detection of brucella melitensis in synovial fluid. | synovial fluid specimens aspirated from patients with arthritis were inoculated into an aerobic peds plus blood culture bottle and monitored by the bactec 9240 instrument for 4 weeks. a total of 1,072 synovial fluid cultures were processed, and 15 (0.14%) were positive for brucella melitensis. a single culture, harboring 1.3 cfu of viable organisms per ml, was missed by the instrument. all 14 positive bactec cultures were detected within 3 to 7 days. | 2001 | 11158139 |
| brucella abortus rb51 and hot saline extract from brucella ovis as antigens in a complement fixation test used to detect sheep vaccinated with brucella abortus rb51. | the efficacy of brucella abortus rb51 and hot saline extract (hse) from brucella ovis as antigens in complement fixation (cf) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with b. abortus strain rb51. for this study, four 5-month-old sheep were vaccinated subcutaneously with 5 x 10(9) cfu of rb51, and two sheep received saline. serum samples collected at different times after vaccination were tested for the presence of antibodies to rb51 by a cf test with rb ... | 2001 | 11139204 |
| limited genetic diversity of brucella spp. | multilocus enzyme electrophoresis (mlee) of 99 brucella isolates, including the type strains from all recognized species, revealed a very limited genetic diversity and supports the proposal of a monospecific genus. in mlee-derived dendrograms, brucella abortus and a marine brucella sp. grouped into a single electrophoretic type related to brucella neotomae and brucella ovis. brucella suis and brucella canis formed another cluster linked to brucella melitensis and related to rhizobium tropici. th ... | 2001 | 11136777 |
| pacemaker infection by brucella melitensis: a rare cause of relapsing brucellosis. | 2000 | 11088098 | |
| [meningitis as the first and only manifestation of brucellosis]. | an 8-year-old girl with no previous symptoms was admitted to hospital with aseptic meningitis syndrome. the girls father is a shepherd and there was a familial antecedent of brucellosis, but the patient did not consume unpasteurized goat milk products nor did she take part in the care of the sheep stock. the clinical evolution did not follow the usual development of a viral or partially treated bacterial meningitis. brucella melitensis was isolated in the cerebrospinal fluid and blood. specific ... | 2000 | 11083975 |
| hemin-binding surface protein from bartonella quintana. | bartonella quintana, the agent of trench fever and a cause of endocarditis and bacillary angiomatosis in humans, has the highest reported in vitro hemin requirement for any bacterium. we determined that eight membrane-associated proteins from b. quintana bind hemin and that a approximately 25-kda protein (hbpa) was the dominant hemin-binding protein. like many outer membrane proteins, hbpa partitions to the detergent phase of a triton x-114 extract of the cell and is heat modifiable, displaying ... | 2000 | 11083791 |
| genetic organisation of the lipopolysaccharide o-antigen biosynthesis region of brucella melitensis 16m (wbk). | brucella spp. are gram-negative, facultative intracellular bacteria that cause a zoonotic world-wide disease. as in other gram-negative bacteria, its s-lps (smooth lipopolysaccharide) is a major determinant of virulence. the brucella melitensis 16m lps o-antigen is a homopolymer of 4-formamido-4,6, dideoxymannose. in this study, the previously cloned 14-kb wbk gene cluster was sequenced, and seven open reading frames (orfs) as well as four insertion sequences were identified. six of the seven or ... | 2000 | 11081580 |
| brucella melitensis dacryoadenitis: a case report. | to present a case of brucellosis-related unilateral dacryoadenitis. | 2000 | 11071036 |
| febrile neutropenia caused by brucella melitensis in a child with hypoplastic acute lymphoblastic leukemia. | 2000 | 11070484 | |
| identification and characterization of in vivo attenuated mutants of brucella melitensis. | brucella melitensis 16m is a gram-negative alpha2-proteobacterium responsible for abortion in goats and for malta fever in humans. this facultative intracellular pathogen invades into and survives within both professional and non-professional phagocytes. signature-tagged mutagenesis (stm) was used to identify genes required for the in vivo pathogenesis of brucella. a library of transposon mutants was screened in a murine infection model. out of 672 mutants screened, 20 were not recovered after a ... | 2000 | 11069678 |
| assessment of a monoclonal antibody-based competitive enzyme linked immunosorbent assay (celisa) for diagnosis of brucellosis in infected and rev. 1 vaccinated sheep and goats. | in this study a celisa for the diagnosis of brucellosis due to b. melitensis in sheep and goats was evaluated and its capability of discriminating vaccinated from infected animals was assessed. information is provided indicating that the celisa has a diagnostic sensitivity (99.4%) and specificity (98.9%) in sheep and goats comparable to that of many standard indirect elisa methods. in addition, the test proved able to distinguish between vaccinated and infected animals with an accuracy of up to ... | 2000 | 11061628 |
| thrombocytopenic purpura associated with brucellosis: report of 2 cases and literature review. | mild hematologic abnormalities are common in the course of human brucellosis; however, they generally resolve promptly with treatment of the disease. occasionally, thrombocytopenia is severe and can be associated with bleeding into the skin (purpura) and from mucosal sites. we describe 2 patients infected with brucella melitensis who presented with thrombocytopenic purpura, and we review 41 additional cases from the literature. patients ranged in age from 2 to 77 years, and both sexes were affec ... | 2000 | 11049768 |
| [gene therapy of chronic infections of the urogenital system using cytotoxic peptides]. | gene therapy of chronic infectious diseases of urogenital tract represents a new perspective field in the modern biological and medical sciences. in the review discuss one of the new directions in gene therapy of urogenital infections caused by mycoplasma: inhibition of mycoplasmal infection after administration of recombinant plasmid vectors, expressed the genes of cytotoxic peptides. | 2000 | 11033890 |
| talking to the patient. | 2000 | 11009144 | |
| characterization of the three ribosomal rna operons rrna, rrnb, and rrnc, from brucella melitensis. | the three brucella melitensis ribosomal rna operons rrna, rrnb, and rrnc were characterized individually. each locus consisted of the 16s rrna gene (rrs), followed by an intergenic spacer containing the trna-ile and trna-ala genes, the 23s rrna gene (rrl), an intergenic spacer devoid of trna genes, the 5s rrna gene (rrf), and an f-met trna gene. the dna sequences were identical over a 6271bp region, diverging 594bp upstream of rrs and immediately downstream of the f-met trna gene. the previously ... | 2000 | 10974571 |
| impaired control of brucella melitensis infection in rag1-deficient mice. | after intranasal inoculation, brucella melitensis chronically infects the mononuclear phagocyte system in balb/c mice, but it causes no apparent illness. adaptive immunity, which can be transferred by either t cells or antibody from immune to naive animals, confers resistance to challenge infection. the role of innate, non-b-, non-t-cell-mediated immunity in control of murine brucellosis, however, is unknown. in the present study, we documented that balb/c and c57bl/6 mice had a similar course o ... | 2000 | 10948160 |
| rapid detection and identification of biological and chemical agents by immunoassay, gene probe assay and enzyme inhibition using a silicon-based biosensor. | a rapid biosensor assay procedure that utilizes biotin streptavidin mediated filtration capture onto nitrocellulose membrane, in conjunction with a silicon-based light-addressable potentiometric sensor (laps) was developed for detection and identification of biological and chemical threat agents. sandwich immunoassays, nucleic acid hybridization assays and enzyme inhibition assays are described. for immunoassays, the lower limits of detection (lod) per 100-microl sample were approximately 5 pg/m ... | 2000 | 10945454 |
| structure and properties of the outer membranes of brucella abortus and brucella melitensis. | the brucellae are gram-negative bacteria characteristically able to multiply facultatively within phagocytic cells and which cause a zoonosis of world-wide importance. this article reviews the structure and topology of the main components (lipopolysaccharide, native hapten polysaccharide, free lipids and proteins) of the outer membranes of brucella abortus and b. melitensis, as well as some distinctive properties (permeability and interactions with cationic peptides) of these membranes. on these ... | 1998 | 10943337 |
| isolation of chlamydia psittaci involved in abortion of goats in mexico: first report. | a group of 35 healthy adult goats ranging from two to six years old were bought from a chlamydiosis and brucellosis-free flock; they were vaccinated against brucella melitensis with rev1 vaccine at reduced doses, and one month later placed in isolation units. the animals were one month pregnant at the moment of purchase, and during the third month of pregnancy 10 out of the 35 dams aborted. necropsy of the aborted fetus and examination of the foetal membranes was performed where no macroscopic l ... | 1997 | 10932720 |
| [brucellosis presenting as acute abdomen]. | usually symptoms of brucellosis are nonspecific and characterized by a wide range of complaints. although the disease in israel is almost exclusively food borne (caused by brucella melitensis in unpasteurized goat milk products) so the main route of infection is the gastrointestinal tract, but gastrointestinal complications are rare, and only sporadic cases of ileitis or colitis have been described. we present a 43-year-old woman with an acute abdomen, probably due to diverticulitis. it was diag ... | 1999 | 10914217 |
| bacterial endocarditis caused by brucella melitensis biotype i. | 2000 | 10893900 | |
| [brucellosis]. | brucellosis is a zoonosis, reported for the first time in 1859 by marston, in malta. it has also been referred to as mediterranean fever, malta fever and bang's disease. its causative agent was isolated by david bruce in 1886; this was brucella melitensis: five different strains were later described: b. abortus, b. suis, b. ovis, b. neotomae and b. canis. recently, in 1994, the isolation of brucella was reported in marine mammals, eventually pathogenic to man and unofficially designated "b. mari ... | 1999 | 10892434 |
| in vitro markers and biological activity in mice of seed lot strains and commercial brucella melitensis rev 1 and brucella abortus b19 vaccines. | four seed lots and fourteen batches of brucella melitensis rev 1 and b. abortus b19 living anti-brucella commercial vaccines obtained from six spanish laboratories were tested in vitro and in vivo in the reference mouse model for quality control. all the strains tested showed the characteristic morphology of their respective rev 1 or b19 reference strains with the exception of three rev 1 strains: seed lot sl2 and commercial vaccine r3, in which giant colonies were predominant, and commercial va ... | 2000 | 10885618 |
| osmotic regulation of cyclic 1,2-beta-glucan synthesis. | in contrast to what happens in agrobacterium: tumefaciens and rhizobium: meliloti, synthesis of periplasmic cyclic 1,2-beta-glucan in brucella: spp. was not inhibited when bacteria were grown in media of high osmolarity. studies performed with crude membrane preparations showed that cyclic 1,2-beta-glucan synthetase of brucella: spp. was not inhibited by 0.5 m kcl or potassium glutamate; concentrations that completely inhibit the osmosensitive enzymes of a. tumefaciens a348 or r. meliloti 102f34 ... | 2000 | 10878137 |
| conservation of seven genes involved in the biosynthesis of the lipopolysaccharide o-side chain in brucella spp. | seven genes of the wb locus of brucella melitensis 16m involved in the biosynthesis of the lipopolysaccharide o-side chain have been recently identified, i.e. wbka, gmd, per, wzm, wzt, wbkb, and wbkc, coding, respectively, for proteins homologous to mannosyltransferase, gdp-mannose 4,6 dehydratase, perosamine synthetase, abc-type transporter (integral membrane protein), abc-type transporter (atpase domain), a hypothetical protein of unknown function, and a putative formyl transferase. the seven ... | 2000 | 10865948 |
| complementation of brucella abortus rb51 with a functional wboa gene results in o-antigen synthesis and enhanced vaccine efficacy but no change in rough phenotype and attenuation. | brucella abortus rb51 is a stable rough, attenuated mutant vaccine strain derived from the virulent strain 2308. recently, we demonstrated that the wboa gene in rb51 is disrupted by an is711 element (r. vemulapalli, j. r. mcquiston, g. g. schurig, n. srirauganathan, s. m. halling, and s. m. boyle, clin. diagn. lab. immunol. 6:760-764, 1999). disruption of the wboa gene in smooth, virulent b. abortus, brucella melitensis, and brucella suis results in rough, attenuated mutants which fail to produc ... | 2000 | 10858205 |
| [a comparative evaluation of different diagnostic agents in the pha in brucellosis]. | 1999 | 10852071 | |
| [incidence, etiology and epidemiology of brucellosis in a rural area of the province of lleida]. | this a prospective study of the incidence, etiology and epidemiological profile of human brucellosis in the regions of pallars jussà y sobirà (lleida) for the 1995-1998 period. | 2000 | 10832390 |
| cutaneous manifestations in brucellosis: case report and review of the literature. | brucellosis is a classical common zoonotic disease of worldwide distribution. skin complications are infrequent and affect less than 5% of patients with brucellosis, although they may occasionally occur during the clinical course of the disease. here, we report a case of a shepherd presenting with fever and a diffuse maculopapular rash due to brucella melitensis infection, and we provide a review of the relevant literature. | 2000 | 10782403 |
| diagnosis and treatment of brucellosis. | 2000 | 10781713 | |
| brucella spondylitis: an important treatable cause of low backache. | this study was undertaken to see if brucella spondylitis existed as a cause of backache in mumbai and to identify the clinical setting in patients of backache where brucella serology is indicated. | 1999 | 10778520 |
| characterization of heat, oxidative, and acid stress responses in brucella melitensis. | brucella melitensis is a facultative intracellular pathogen which is able to survive and replicate within phagocytic cells. therefore, it has to adapt to a range of different hostile environments. in order to understand the mechanisms of intracellular survival employed by virulent b. melitensis 16m, an initial approach consisting of analysis of the differences in patterns of protein synthesis in response to heat, oxidative, and acid ph stresses by two-dimensional (2-d) polyacrylamide gel electro ... | 2000 | 10768994 |
| brucella bacteraemia: clinical and laboratory observations in 160 patients. | to describe the clinical, serological, and prognostic features of bacteraemic brucellosis in an endemic region. | 2000 | 10762113 |
| deep venous thrombosis associated with acute brucellosis--a case report. | vascular complications, including arteries and veins associated with brucella infection, have rarely been reported. to date, only three cases of deep venous thrombosis (dvt) of the lower extremities associated with brucellosis have been previously reported. the authors describe another case of dvt of the right leg in association with acute brucella infection. since infection with brucella may be asymptomatic, and the manifestations of acute brucellosis are exceedingly nonspecific, and in view of ... | 2000 | 10744014 |
| exposure of hospital personnel to brucella melitensis and occurrence of laboratory-acquired disease in an endemic area. | in 1997, 7 cases of laboratory-acquired brucella melitensis infections were detected among the hospital personnel of a medical centre serving an endemic area in southern israel. although the onset of symptoms in 6 of the 7 patients occurred during a 2-week period, suggesting a point source exposure, biotype analysis showed that the outbreak was caused by 3 different b. melitensis serovars, indicating multiple exposures. review of the laboratory records showed that during 1997, the microorganism ... | 2000 | 10716074 |
| pathological case of the month. disseminated brucellosis. | 2000 | 10710035 | |
| brucella melitensis infection discovered in cattle for first time, goats also infected. | 2000 | 10707672 | |
| [the epidemiological trend of brucellosis in the provinces of sicily]. | the epidemiological trend of brucellosis in italy has been uneven over the last few years since there was a decrease in incidence in some regions and an increase in others, including sicily. the peak was reached in 1997 when 59% of the cases were reported in sicily alone. appropriate intervention strategies are therefore needed both as regards the general population and exposed workers in order to reduce the spread of this disease. | 1999 | 10703194 |
| molecular characterization of brucella strains isolated from marine mammals. | recently, gram-negative bacteria isolated from a variety of marine mammals have been identified as brucella species by conventional phenotypic analysis. this study found the 16s rrna gene from one representative isolate was identical to the homologous sequences of brucella abortus, b. melitensis, b. canis, and b. suis. is711-based dna fingerprinting of 23 isolates from marine mammals showed all the isolates differed from the classical brucella species. in general, fingerprint patterns grouped by ... | 2000 | 10699036 |
| topology prediction of brucella abortus omp2b and omp2a porins after critical assessment of transmembrane beta strands prediction by several secondary structure prediction methods. | in order to propose a reliable model for brucella porin topology, several structure prediction methods were evaluated in their ability to predict porin topology. four porins of known structure were selected as test-cases and their secondary structure delineated. the specificity and sensitivity of 11 methods were separately evaluated. our critical assessment shows that some secondary structure prediction methods (phd, dsc, sopma) originally designed to predict globular protein structure are usefu ... | 2000 | 10698111 |
| brucellosis in a mother and her young infant: probable transmission by breast milk. | brucellosis, although primarily a zoonotic infection, is also a threat for human health. infection can be transmitted to humans through direct contact with infected animals, products of conception, or animal discharges, and through consumption of potentially infected milk, milk products, or meat. human-to-human transmission is rare. there have been case reports of transmission via blood transfusion and bone marrow transplantation from infected donors. sexual intercourse is a possible means of tr ... | 2000 | 10689217 |
| lipopolysaccharides of brucella abortus and brucella melitensis induce nitric oxide synthesis in rat peritoneal macrophages. | smooth lipopolysaccharide (s-lps) and lipid a of brucella abortus and brucella melitensis induced the production of nitric oxide (no) by rat adherent peritoneal cells, but they induced lower levels of production of no than escherichia coli lps. the participation of the inducible isoform of no synthase (inos) was confirmed by the finding of an increased expression of both inos mrna and inos protein. these observations might help to explain (i) the acute outcome of brucella infection in rodents, ( ... | 2000 | 10679001 |
| iliopsoas bursitis due to brucella melitensis infection--a case report. | 1999 | 10622492 | |
| [increased production of the protein antigen of brucella melitensis in escherichia coli k-12 cells]. | the production of brucella melitensis protein antigen with molecular weight of 38 kd in escherichia coli k-12 cell lysates has been studied by immunoblotting with various antisera. e. coli strains differed by the vector plasmid and the size of b. melitensis 565 dna fragment with 38 kd protein gene, cloned in this plasmid. the immunoblotting analysis detected increased production of 38 kd protein in the recombinant gse830 strain in comparison with the b. melitensis strain 565, from which the gene ... | 1999 | 10621933 |
| chronic intracerebral brucella abscess. case illustration. | 2000 | 10616102 | |
| [liver abscess probably caused by brucella]. | 1999 | 10614086 | |
| effects of opsonization and gamma interferon on growth of brucella melitensis 16m in mouse peritoneal macrophages in vitro. | entry of opsonized pathogens into phagocytes may benefit or, paradoxically, harm the host. opsonization may trigger antimicrobial mechanisms such as reactive oxygen or nitric oxide (no) production but may also provide a safe haven for intracellular replication. brucellae are natural intramacrophage pathogens of rodents, ruminants, dogs, marine mammals, and humans. we evaluated the role of opsonins in brucella-macrophage interactions by challenging cultured murine peritoneal macrophages with bruc ... | 2000 | 10603396 |
| cold agglutinins as a fast clue to brucellosis? | 1999 | 10593136 | |
| epidemiology and control of brucellosis in ruminants from 1986 to 1996 in malta. | the epidemiology and control of brucella melitensis in malta was analysed using herd test data made available by the veterinary service of malta. the eradication scheme commenced in 1987 with the introduction of a test and slaughter scheme using the rose bengal test. herds registered with malta dairy products limited (mdp) showed a herd prevalence of 23% in 1987 which fell to less than 1.5% by 1993. prevalence rose to 13% in 1995. herds not delivering milk to the mdp showed an initial herd preva ... | 1999 | 10588008 |
| brucellar spondylitis: review of 35 cases and literature survey. | thirty-five patients aged 14-74 years (average, 54 years) who had brucellar spondylitis were treated between january 1991 and december 1997. the time from onset of symptoms to diagnosis of spondylitis ranged from 1 week to 8 months (median, 9 weeks). back or neck pain (100% of patients), fever (66%), and constitutional symptoms (57%) were the most common symptoms. cultures of blood specimens from 26 patients (74%) were positive for brucella melitensis. the duration of antimicrobial therapy (medi ... | 1999 | 10585793 |
| comparison of e test to microdilution for determining in vitro activities of antibiotics against brucella melitensis. | 1999 | 10577353 | |
| [acute cholecystitis during systemic brucellosis]. | 1999 | 10568164 | |
| brucellosis outbreak: analysis of risk factors and serologic screening. | israel is one of the mediterranean countries in which brucellosis is endemic. as recently there has been a brucellosis outbreak in a kibbutz, the aim of this study is to identify asymptomatic infected kibbutz members, and to delineate the manner of infection in this setting. therefore, all the asymptomatic kibbutz members were screened by the rose bengal test for brucellosis, while both patients and healthy members were asked to fill in a questionnaire in order to pinpoint the manner of infectio ... | 1999 | 10567679 |
| supramolecular organization of photosystem ii and its light-harvesting antenna in partially solubilized photosystem ii membranes. | we present an extended analysis of the organization of green plant photosystem ii and its associated light-harvesting antenna using electron microscopy and image analysis. the analysis is based on a large dataset of 16 600 projections of negatively stained psii-lhcii supercomplexes and megacomplexes prepared by means of three different pretreatments. in addition to our previous work on this system [boekema, e.j., van roon, h., calkoen, f., bassi, r. and dekker, j.p. (1999) biochemistry 38, 2233- ... | 1999 | 10561584 |
| protection of mice against brucellosis by vaccination with brucella melitensis wr201(16mdeltapurek). | human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of the conjunctiva or traumatized skin by infected animal products. a vaccine to protect humans from occupational exposure or from zoonotic infection in areas where the disease is endemic would reduce an important cause of morbidity worldwide. vaccines currently used in animals are unsuitable for human use. we tested a live, attenuated, purine-auxotrophic mutant strain of brucella melitensis ... | 1999 | 10531243 |
| acute calcular cholecystitis in a patient with brucellosis. | 1999 | 10517200 | |
| [brucella melitensis biovar 3 in argentina?]. | 1999 | 10509416 | |
| divergence paralysis & intracranial hypertension due to neurobrucellosis. a case report. | case report: a 22 year old female presented with sudden onset of uncrossed diplopia at distance, intracranial hypertension, esotropia and was evaluated. microbiological tests of csf and sera showed for brucellosis and the patient received therapy for this and her intracranial hypertension. the papilledema, headache, esotropia and diplopia all disappeared after therapy. conclusions: diagnostic tests for brucella must be considered for patients who have divergence palsy and papilledema, especially ... | 1999 | 10506689 |
| brucella spp. peritonitis. | 1999 | 10492334 | |
| spontaneous bacterial peritonitis due to brucella melitensis in a cirrhotic patient. | 1999 | 10484037 | |
| bilateral mammary abscess due to brucella melitensis. | brucellosis is an important zoonosis, which is widespread as endemic cases in turkey. unusual clinical features and complications can sometimes lead to diagnostic difficulties. we describe here a case of a pregnant woman with bilateral mammary abscesses due to brucella melitensis. | 1999 | 10482066 |
| identification of an is711 element interrupting the wboa gene of brucella abortus vaccine strain rb51 and a pcr assay to distinguish strain rb51 from other brucella species and strains. | brucella abortus vaccine strain rb51 is a natural stable attenuated rough mutant derived from the virulent strain 2308. the genetic mutations that are responsible for the roughness and the attenuation of strain rb51 have not been identified until now. also, except for an assay based on pulsed-field gel electrophoresis, no other simple method to differentiate strain rb51 from its parent strain 2308 is available. in the present study, we demonstrate that the wboa gene encoding a glycosyltransferas ... | 1999 | 10473532 |
| vertebral osteomyelitis in northern spain. report of 62 cases. | the records of 62 patients with clinical and radiographic evidence of vertebral osteomyelitis and positive bacteriological diagnosis, seen between 1979 and 1996, were reviewed in order to gather data on the epidemiology and the clinical pattern displayed by patients with this condition in northern spain. | 1999 | 10464555 |
| outer membrane proteins omp10, omp16, and omp19 of brucella spp. are lipoproteins. | the deduced sequences of the omp10, omp16, and omp19 outer membrane proteins of brucella spp. contain a potential bacterial lipoprotein processing sequence. after extraction with triton x-114, these three proteins partitioned into the detergent phase. processing of the three proteins is inhibited by globomycin, a specific inhibitor of lipoprotein signal peptidase. the three proteins were radioimmunoprecipitated from [(3)h]palmitic acid-labeled brucella abortus lysates with monoclonal antibodies. ... | 1999 | 10456959 |
| use of mass vaccination with a reduced dose of rev 1 vaccine for brucella melitensis control in a population of small ruminants. | mass vaccination with reduced dose 1/50 rev 1 strain live vaccine (1-2 10(9) colony forming units), administered subcutaneously, over a four and a half year period reduced the prevalence of brucella melitensis in kuwait's small ruminant population from 5.8% in 1993 to 2.02% in 1997. serological test results using the rose bengal plate test, rivanol agglutination test and complement fixation showed no evidence of persistence of positive serology in animals nine or more months after vaccination. q ... | 1999 | 10445249 |
| the effects of electron and chemical ionization modes on the ms profiling of whole bacteria. | free fatty acid profiling of whole bacteria [francisella tularensis, brucella melitensis, yersinia pestis, bacillus anthracis (vegetative and sporulated), and bacillus cereus] was carried out with direct probe mass spectrometry under 70-ev electron ionization (ei) and isobutane chemical ionization in both the positive (ci+) and negative modes (ci-). electron ionization produced spectra that contained molecular ions and fragment ions from various free fatty acids. spectra acquired with isobutane ... | 1999 | 10439512 |
| in vitro activities of five new antimicrobial agents against brucella melitensis. | 1999 | 10418765 | |
| bilateral dacryoadenitis associated with brucellosis. | 1999 | 10406164 | |
| who consultation on the development of new/improved brucellosis vaccines. 17 december 1997, geneva, switzerland. | 1998 | 10403040 | |
| an adolescent pakistani girl with chronic meningitis. | 1999 | 10396591 | |
| increasing resistance of brucellae to co-trimoxazole. | 1999 | 10383374 | |
| investigation into the role of the response regulator ntrc in the metabolism and virulence of brucella suis. | during infection, brucella species have to adapt to a range of different environments. environmental sensing in bacteria often involves the concerted action of two-component regulatory systems consisting of sensor and response regulator components. in this study, we identified, cloned and sequenced four independent response regulator gene fragments from brucella melitensis. one amplified gene fragment showed nearly 90% identity to the response regulator subfamily of ntrc transcriptional activato ... | 1999 | 10373105 |