Publications

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[localization of the antigenic material from densonucleosis virus during infection of galleria mellonella l]. 19684885384
[bacterial infection due to salmonella typhimurium in an invertebrate, galleria mellonella l]. 19684871974
neuronal modulation of photoreceptor activity in the bee moth galleria mellonella. 19744847282
polypeptides in tipula iridescent virus (tiv) and in tiv-infected hemocytes of galleria mellonella (l.) larvae. 19744844416
the fate of exotoxin of bacillus thuringiensis in galleria mellonella caterpillars. 19744825257
galleria mellonella nerve cords in vitro: stage-specific survival and differential responsiveness to beta-ecdysone. 19744819573
mechanisms of action of co2 on the visual response of galleria mellonella. 19744819571
components of the erg of the moth, galleria mellonella. 19744811212
microtubules and tracheole migration in wing disks of galleria mellonella. 19734789610
metamorphic shortening of interganglionic connectives of galleria mellonella (lepidoptera) in vitro: stimulation by ecdysone analogues. 19734773932
studies on insect bacteriolytic enzymes. i. lysozyme in haemolymph of galleria mellonella and bombyx mori. 19734717478
studies on insect bacteriolytic enzymes. i. lysozyme in haemolymph of galleria mellonella and bombyx mori. 19734712882
radiation effects on the hemolymph free amino acid pool of the honeybee prepupa (apis mellifera) and the waxmoth larva (galleria mellonella). 19734703888
developmental changes in the rate of oxygen consumption in egg vesicles of galleria mellonella (lepidoptera). 19724640879
antibiotic properties of bacillus subtilis strain no. 53 isolated from the intestinal flora of galleria mellonella l. larvae. 19734633785
[amino acid makeup and properties of the polyhedral protein of the virus of nuclear polyhedrosis in galleria mellonella]. 20034597929
the effects of juvenile hormone on the prothoracic glands in galleria mellonella l. ii. the jha-induced changes at the light microscopic level. 19744464728
fat body of galleria mellonella during metamorphosis. cytochemical and ultrastructural studies. 19744455576
the influence of illuminiation conditions on growth and development of galleria mellonella. 19744455363
bacteria associated with the nematode neoplectana carpocapsae and the pathogenicity of this complex for galleria mellonella larvae. 19744443607
subviral infectivity in nuclear polyhedrosis of the great wax moth (galleria mellonella l.). 19724404101
the effect of subsequent passage on the activation of densonucleosis virus in galleria mellonella l. 20144392081
[separation of hemolymph proteins in prepupae and pupae of galleria mellonella l. by means of gel filtration on sephadex g-200. determination of their isoelectric points and their molecular weights]. 19694390024
cyclic nucleotide binding and protein kinase in the central nervous system of galleria mellonella (l.). 19744367845
insect neurometamorphosis. v. fine structure of axons and neuroglia in the transforming interganglionic connectives of galleria mellonella (l.) (lepid optera). 19724338500
pathogenicity of bacillus cereus for insects. ii. toxicity of phospholipase c for galleria mellonella. 19724337759
selective blocking of somatic neuromuscular transmission in the larval wax moth, galleria mellonella, by bromolysergic acid diethylamide. 19714328942
determination of the molecular weight of proteins, esterases, and basic phospho-monoesterases in the hemolymph of galleria mellonella l. prepupa and pupa using a thin-layer chromatography. 19694306706
innervation and neural excitation of ventral muscle fibres of the larva of the waxmoth, galleria mellonella. 19694305886
the mechanism of pathogenicity of pseudomonas aeruginosa. v. isolation and properties of the proteinases toxic for larvae of the greater wax moth galleria mellonella l. 19684300545
influence of starvation on metabolic activities of the midgut epithelium of galleria mellonella larvae. 19744280375
[observations and experimental data on the functioning of the ovaries in adult galleria mellonella l. (lepidoptera, pyralidae)]. 19664228011
studies on the 3'-terminal sequences of the large ribosomal ribonucleic acid of different eukaryotes and those associated with "hidden" breaks in heart-dissociable insects 26s ribonucleic acid.the 3'-terminal sequences associated with the large rrna complex from a range of eukaryotes were determined after pancreatic or t(1)-ribonuclease digestion of rna terminally labelled with [(3)h]isoniazid. in all higher eukaryotes examined except drosophila melanogaster, the 3'-terminal sequences y-g-u(oh) and g-c-u(oh) were demonstrated for the large rna component(s) and for 6s rna respectively. the 3'-terminal sequence of saccharomyces cerevisiae 26s rna was y-g-u(oh) and that of 6s rna y-a-u-u ...19744219141
[data on the organogenesis of the female genital tract of the pyralid lepidoptera galleria mellonella l. obtained after implantation of the ovaries in castrated and non-castrated larvae]. 19734211004
regulation of larval corpora allata in galleria mellonella. 19744153842
ultrastructural changes in larval wax moth, galleria mellonella, midgut and silk gland after exposure to chlorcyclizine, phenobarbital, or promazine. 19724144199
the effects of juvenile hormone on the prothoracic glands in galleria mellonella l. i. morphology of the glands in the course of the postembryonic development. 19744142915
the structure of the ovariole wall in galleria mellonella. 19734130455
the influence of starvation on ribonucleic acid and protein synthesis in the mid-gut epithelium of galleria mellonella larvae. 19734130447
a cytological and immunological study of tipula iridescent virus-infected galleria mellonella larval hemocytes. 19734120908
insect mucosubstances. 3. some mucosubstances of the nervous systems of the wax-moth (galleria mellonella) and the stick insect (carausius morosus). 19714110368
[characteristics of nuclear dna of a lepidoptera, the great wax moth galleria mellonella, using reassociation kinetics].reassociation kinetics of great wax moth--galleria mellonella nuclear dna short fragments was studied within the c0t values of 0.02 and 10(4). the least squares analysis of the experimental curve obtained revealed two components with c0t1/2 = 0.72 and c0t1/2 = 750 appropriately. the fraction of the component with c0t1/2 = 0.72 in the genome of great wax moth is 22% and that of the other one is 61%. there is also a rapid component which renatured at the c0t values less then 0,02. the fraction of ...20064033638
in vitro host range of five baculoviruses in lepidopteran cell lines.the in vitro host range of five nuclear polyhedrosis viruses (npv) was assessed in five lepidopteran cell lines from three genera. multiple-enveloped baculoviruses of autographa californica (acmnpv), trichoplusia ni (tnmnpv), and galleria mellonella (gmmnpv) replicated in cells of t. ni, spodoptera frugiperda, and heliothis virescens to a titer of approximately 10(7) tcid50/ml. the multiple-enveloped baculovirus of s. frugiperda (sfmnpv) replicated only in s. frugiperda cells. the single-envelop ...19853988484
suppression of high-affinity ligand binding to the major glutathione s-transferase from galleria mellonella by physiological concentrations of glutathione.the major glutathione s-transferase from larvae of galleria mellonella binds a number of synthetic triphenylmethane dyes with dissociation constants of the order of 10(-6) m or less. the organ distribution of the enzyme activity does not parallel the uptake of such dyes by the insect's organs in vivo. the affinity of the protein for such dyes is decreased by about an order of magnitude by the presence of glutathione in normal physiological concentration. this appears to be the cause of this prot ...19863954738
annual changes in the greater wax moth (galleria mellonella l.) development and sensitivity to juvenilizing treatments.the greater wax moth (galleria mellonella l.) larvae reared in constant conditions showed endogenous annual changes in the sensitivity to juvenilizing treatments, i.e. cooling and jha administration. also control, untreated larvae showed annual changes in normal development. the number of spontaneously appearing extra-larval molts, the number of animals entering the state of permanent larva, as well as the sex-ratio in galleria population changes with respect to the season of the year. the possi ...19853870854
juvenile-hormone-binding protein from the hemolymph of galleria mellonella (l). isolation and characterization.a juvenile-hormone-binding protein (jhbp) has been isolated from galleria mellonella hemolymph by gel filtration, phosphocellulose chromatography, and by chromatofocusing. the isolated protein is homogeneous as judged by column chromatography and gel electrophoresis in the presence and absence of denaturing agent. it has a relative molecular mass of 32,000, stokes radius 2.4 nm, sedimentation coefficient of 2.3 s, molar absorption coefficient at 280 nm epsilon = 2.34 x 10(4) m-1 cm-1, and is com ...19873830162
ultracytochemical localization and microprobe quantitation of calcium stores in the insect oocyte.detection of calcium in the follicles of galleria mellonella (lepidoptera) was performed using two cytochemical methods. calcium precipitation was obtained either with ammonium oxalate (ao) or with n,n-naphtaloylhydroxylamine (nha). in both cases the x-ray "on line" analysis monitored the presence of calcium in the oocytes, which was correlated with the accumulation of yolk spheres. concentration of calcium in oocytes filled with yolk and treated with ao amounted to 9 mmoles per 1,000 g tissue w ...20133744899
heterorhabditis spp. and steinernema (= neoaplectana) spp.: temperature, and aspects of behavior and infectivity.the infectivity of several species/strains of nematodes of the genera heterorhabditis and steinernema for postfeeding, 3rd instar larvae of the sheep blowfly, lucilia cuprina, was tested in sand at various temperatures. of the two genera, the steinernematids were more active at lower temperatures and parasitized l. cuprina over a greater temperature range. the temperature range of infectivity for l. cuprina differed between nematodes of the same genus and between strains of the same species. par ...19863743713
a cobra venom factor (cvf)-induced c3 convertase activity in the hemolymph of galleria mellonella.a cobra venom factor (cvf)-induced c3 convertase has been generated from the hemolymph of galleria mellonella. cvf was immobilized on sepharose 4b and treated with cell-free hemolymph obtained from either unvaccinated g. mellonella larvae or larvae immunized with formalized pseudomonas aeruginosa. the c3-cleaving activity was detected by the ability to cleave the alpha-chain of bovine c3 in a manner analogous to the cvf-induced mammalian c3 convertase, cvf,bb. the insect-derived c3 convertase fo ...19873647909
further characterization of an alkaline protease activity associated with iridescent virus type 6. brief report.iridescent virus type 6 infecting galleria mellonella larvae contained an associated alkaline protease activity which appeared to be tightly bound to the virions and essentially localized on the outside of the viral particle. under alkaline conditions the virus was degraded by proteolytic cleavage of viral envelope proteins. proteolytic activity was not present in virus propagated in a permissive insect cell line or when purified from isolated larval fat-bodies instead from whole larvae. the res ...19863511892
preliminary characterization of toxins from the straw itch mite, pyemotes tritici, which induce paralysis in the larvae of a moth.homogenates of whole mites (pyemotes tritici) paralyze larvae of the greater wax moth galleria mellonella. injection of these homogenates into larvae produces symptoms identical to those obtained by bites from female mites. since the paralytic activity is destroyed by heat and proteolytic enzymes and retained during dialysis, the toxic compounds appear to be proteins. two protein fractions which differ both in molecular weight and toxicity were found following gel filtration of whole mite extrac ...19883363564
the meisenheimer complex of glutathione and trinitrobenzene. a potent inhibitor of the glutathione s-transferase from galleria mellonella.1. the meisenheimer complex formed between reduced glutathione and 1,3,5-trinitrobenzene is characterised by an extinction coefficient at 470 nm of 20400 and by an association constant at ph 9.18 of 42 l.mol-1. 2. trinitrobenzene is a moderately good inhibitor of the glutathione s-transferase from larvae of the moth galleria mellonella. it acts by competition with the electrophilic substrate. at ph 7.4, it has a ki value of 10 microm. its mode of inhibition with respect to gsh appears to be non- ...19883342082
synthesis and binding affinity of an iodinated juvenile hormone.the synthesis of the first iodinated juvenile hormone (jh) in enantiomerically enriched form is reported. this chiral compound, 12-iodo-jh i, has an iodine atom replacing a methyl group of the natural insect juvenile hormone, jh i, which is important in regulating morphogenesis and reproduction in the lepidoptera. the unlabeled compound shows approximately 10% of the relative binding affinity for the larval hemolymph jh binding protein (jhbp) of manduca sexta, which specifically binds natural 3h ...19883335551
cold-induced supernumerary moults of galleria mellonella larvae as bioassay for anti-juvenile hormone compounds. 20083213548
reactions of hemocytes of immune and non-immune galleria mellonella larvae to proteus mirabilis.immune larval galleria mellonella removed live proteus mirabilis from the hemolymph less effectively than did non-immune larvae. this was attributed to a decline in total hemocyte counts, levels of plasmatocytes and granulocytes and hemocyte adhesion capacity. immune serum possessed factors which reduced bacterial adhesion to the hemocytes. this was not due to altering bacterial surfaces but rather to irreversible suppression of hemocyte activity.19873109970
[transposition and reversion of mutations induced in drosophila by viral dna].injection of solutions of highly polymerized dna isolated from nuclear polyhedrosis virus of galleria mellonella into adult males induced with a considerable frequency visible mutations, two of which were studied in detail. they were detected in about 30 000 flies in the progeny of treated males. much less than notched-wings much greater than (ndw, chromosome 3, location 87.9, dominant) independently arose 12 times, much less than thickened-veins much greater than (thi, chromosome 2, location 71 ...19863006304
transposon-mediated mutagenesis of a baculovirus.spontaneous mutants of insect nuclear polyhedrosis viruses of autographa californica (acmnpv) and galleria mellonella (gmmnpv) were analyzed. these mutants produce few polyhedra in infected cells and have insertions of host cell dna. all the insertions mapped to two adjacent sites in the genome. the junctions between two host insertions and the viral dna were sequenced. one of the insertions contained a perfect 7-bp inverted terminal repeat, and had caused a direct duplication of 4 bp of viral d ...19852992159
ultrastructural alterations of the egg chambers of galleria mellonella l. (lepidoptera) developing after reserpine administration.in the egg chambers of the wax moth galleria mellonella l., developing after application of reserpine, a multilayered epithelium was observed through all the developmental stages. this epithelium consisted of "light" cells loaded with intracellular organelles. no "dark" cells, characteristic of the control material, were observed. all cells of the egg chambers of reserpine-treated insects were characterized mostly by an abundance of microtubules, strong vacuolation of cytoplasm, and dilation of ...19852938376
[effect of temperature on the infectivity of the iridovirus and densonucleosis virus of blood-sucking mosquitoes].mosquito densonucleosis virus and iridovirus were used to study the influence of temperature on their infectivity. biological assays of mosquito densonucleosis virus were carried out in larvae of i-ii instar of aedes aegypti mosquitoes, those of mosquito iridovirus in larvae of galleria mellonella. the rate of inactivation was found to be directly dependent on the dose of the heat treatment. treatment at 60 degrees c led to complete loss of iridovirus infectivity; the virus activity declined con ...20102905849
comparisons of host cell dna insertions and altered transcription at the site of insertions in few polyhedra bacilovirus mutants.few polyhedgra (fp) mutants of autographa californica nuclear polyhedrosis virus (acnpv) and the closely related strain galleria mellonella (gm)npv have been reported which contain trichoplusia ni host cell dna sequences inserted into the viral genome between map units 35.0 and 37.7. new fp mutants are described with alterations of the hindiii-i restriction enzyme fragment (33.8 to 37.7 map units) of acnpv, either deletions of viral dna sequences or insertions of spodoptera frugiperda host cell ...19882829419
identification and distribution of a proctolin-like neuropeptide in the nervous system of the gypsy moth, lymantria dispar, and in other lepidoptera.although the neuropeptide proctolin has important functions in many arthropods, it is reported to be absent in lepidoptera. its possible occurrence in these insects was reinvestigated by bioassays of hplc fractions and immunocytochemistry. a proctolin-like substance was recovered from the frontal and subesophageal ganglia of lymantria dispar. this substance has the same chromatographic retention time as proctolin; enzymatic degradation indicates that it is a peptide; it is bound by proctolin ant ...19892732362
transposon mutagenesis of baculoviruses: analysis of tfp3 lepidopteran transposon insertions at the fp locus of nuclear polyhedrosis viruses.we report the complete sequences of two representatives of the tfp3 transposable element family of the lepidopteran, trichoplusia ni. these elements were isolated as insertions mobilized from the lepidopteran host genome into two closely related nuclear polyhedrosis viruses (npv) during infection. both elements inserted within the 500-bp fp locus of the respective viral genomes (map units 36.0 to 37.0), causing a distinctive plaque morphology phenotype and the loss of a 25-kda viral-specific pro ...19892553540
transposon mutagenesis of baculoviruses: analysis of trichoplusia ni transposon ifp2 insertions within the fp-locus of nuclear polyhedrosis viruses.the transposable ifp2 element of trichoplusia ni was originally isolated as a host dna insertion in spontaneous fp mutants of galleria mellonella or autographa californica nuclear polyhedrosis viruses (npvs). the termini of ifp2 insertions from five independently isolated fp mutants were sequenced. in all cases ifp2 is flanked by 13-bp terminal inverted repeats and has additional inverted repeats of 19 bp in length located asymmetrically with respect to the ends of the element. insertion of ifp2 ...19892549707
the hemolytic activity of galleria mellonella hemolymph.a hemolytic activity was identified in the hemolymph of normal and immune galleria mellonella larvae. the hemolysin was active against sheep, human, guinea pig, and rabbit erythrocytes. hemolysis occurred in the presence of 0.04m edta. vaccination of the larvae with formalized pseudomonas aeruginosa increased the hemolytic activity. the increase, and subsequent decline of this activity paralleled the pattern of induced in vivo antibacterial activity that is characteristic of the insect's immune ...19892506085
effects of selected carbohydrates and the contribution of the prophenoloxidase cascade system to the adhesion of strains of pseudomonas aeruginosa and proteus mirabilis to hemocytes of nonimmune larval galleria mellonella.the adhesion of strains of pseudomonas aeruginosa and proteus mirabilis to the plasmatocytes and granular cells of nonimmune larval galleria mellonella was influenced by and varied with the type of carbohydrate. laminarin enhanced prophenoloxidase activation and bacterial adhesion to the hemocytes whereas sucrose suppressed both activities. for all other sugars there was no correlation between bacterial adhesion to the hemocytes and phenoloxidase activity. it is proposed that bacterial adhesion ...19892501016
adherence patterns and virulence for galleria mellonella larvae of isolates of serratia marcescens. 19902405061
plasmid transfer between strains of bacillus thuringiensis infecting galleria mellonella and spodoptera littoralis.to determine the possibility of plasmid transfer occurring between strains of bacillus thuringiensis in infected lepidopterous larvae, galleria mellonella and spodoptera littoralis were infected with two or more strains of b. thuringiensis and the resulting bacteria from the dead insects were examined for plasmid transfer. transfer rates of plasmids coding for crystal production and tetracycline resistance were high, reaching levels similar to those obtained in laboratory broth cultures. transfe ...19902383006
expression of densonucleosis virus gmdnv in galleria mellonella larvae: size analysis and in vitro translation of viral transcription products.the rna of densonucleosis virus type 1 (gmdnv), isolated from gmdnv-infected galleria mellonella larvae, was shown by northern blotting to contain five polyadenylated, viral-specific rna species with sizes of 1.8, 2.4, 3.5, 4.0, and 5.0 kb. poly(a)-containing rna from whole larvae and hybrid-selected viral rna were translated in a rabbit reticulocyte lysate system and the translation products were coelectrophoresed with proteins extracted from cscl-purified virus. all four virion-associated prot ...19902273284
restriction maps and sequence homologies of two densovirus genomes.the genomes of junonia coenia densonucleosis virus (jcdnv) and galleria mellonella densonucleosis virus (gmdnv) were analysed by restriction endonuclease analysis and southern blot hybridization. a total of 37 and 33 restriction sites were mapped on jcdnv and gmdnv dna, respectively. bgli, haeii and bsteii were site-specific for jcdnv dna, and bglii and clai for gmdnv dna. the two genomes had nearly identical maps for several restriction endonucleases and southern blot hybridization using a tota ...19902230740
implication of mitochondria in the replication of nodamura virus in larvae of the lepidoptera, galleria mellonella (l.) and in suckling mice.the replication of nodamura virus, type species of the family nodaviridae, takes place principally into the interfibrillar spaces of the sarcoplasm in close association with mitochondria in the infected muscles of both g. mellonella and suckling mice. the most striking event in infection of g. mellonella muscle cells was the aggregation and shape modification of numerous mitochondria (elongation, interdigitation, and vesiculation) at an early stage of infection. these clusters of mitochondria we ...19902222183
cane sugar factor as an inducing agent of immunity in galleria mellonella.the injection of cane sugar factor (csf) into galleria mellonella larvae results in an immune response similar to that produced by a formalized pseudomonas aeruginosa vaccine. vaccination with csf is followed by: an increase in the ld50 of pseudomonas aeruginosa; an in vivo protective response to p. aeruginosa the development of which can be inhibited by cobra venom factor (cvf); an antibacterial activity in hemolymph 24 h after the injection of csf; the development of a transferrable immune res ...19902128279
conformational change of the haemolymph juvenile-hormone-binding protein from galleria mellonella (l).a juvenile-hormone-binding protein (juvenile-hormone carrier), isolated from galleria mellonella haemolymph, was treated with trypsin, chymotrypsin, carboxypeptidase a and subtilisin. among these enzymes, only subtilisin was able to affect juvenile-hormone-binding activity of this protein. with sds/page it was shown that juvenile-hormone-binding protein, a 32-kda peptide, is first slowly converted into a 30-kda molecule, then into two or three smaller-molecular-mass species (20-25 kda), which in ...19911935932
inhibition by inorganic anions of glutathione s-transferases from insect and mammalian sources.glutathione s-transferases 1-1, 3-3, 3-4 and 4-4 from rat liver and the major glutathione s-transferase from the wax moth (galleria mellonella) are all inhibited by several simple inorganic anions. for each of 3-3, 3-4 and the insect enzyme, the order of inhibitory potency was clo4- greater than or equal to scn- greater than i- greater than no3- greater than br-. a more limited range of anions was tested on the isoenzymes 1-1 and 4-4, but the same trend was apparent. values for ki ranged from ab ...19911883329
in vitro bioassay for the brain allatotropic hormone of galleria mellonella (lepidoptera).an in vitro sensitive bioassay for the galleria mellonella brain allatotropic hormone (atth) was developed. this assay measures the rate of juvenile hormone (jh) synthesis in corpora cardiacacorpora allata complex (cc-ca) stimulated in vitro by atth released from the brain during short-term in vitro incubation, or by atth extracted from the tissue with methanol. cc-ca of the late vith instar (vi3) larvae were used for assessment of atth. the maximum activation of test cc-ca by atth occurred at a ...19911813322
hydrolytic degradation of juvenile hormones in haemolymph and corpora allata of galleria mellonella (l).hydrolytic rates of juvenile hormones (jhs) i, ii and iii by the corpora cardiaca-corpora allata complex (cc-ca) and by the haemolymph of galleria mellonella remain in the same order (iii greater than i greater than ii in cc-ca and i greater than iii greater than ii in haemolymph) throughout the last larval instar. haemolymph hydrolytic activity shows peak at the end of feeding when 80 pmol jh i versus 15 pmol jh ii is degraded per 1 microliter and minute; hydrolysis rapidly declines in the apol ...19911799112
hormonal regulation of protein synthesis in the silk gland of galleria mellonella.synthesis of the low molecular mass silk proteins of 24 and 30 kda in the last larval instar of galleria mellonella starts between 24 and 48 h; synthesis of the former protein significantly preceding that of the latter. posterior silk glands (psg) from day-1 last instar larvae are transiently sensitive in vitro to exogenous 20-hydroxyecdysone (20-he), which stimulates transcription of the 24 kda protein gene and induces transcription of the gene for the 30 kda protein. the glands from the day -3 ...19911796707
induction of immunity by latex beads and by hemolymph transfer in galleria mellonella.injection of sterile latex beads into the hemocoel of last instar larvae of galleria mellonella provoked a strong defense reaction. cellular defense by hemocytes was followed by enhanced antibacterial activity in hemolymph. latex-injected insects showed increased survival rates after a challenge injection with high doses of bacteria. factors which stimulate the production of antibacterial activity could be demonstrated soon after injection by transfer of hemolymph from preinjected to untreated l ...19911773849
a transfer membrane method for in situ detection and quantification of trehalase.a method for the detection and quantification of trehalase activity (ec 3.2.1.28) by immobilization to a membrane support has been developed. protein samples partly enriched for porcine and galleria mellonella wax moth larvae trehalase activities were fractionated by polyacrylamide gel electrophoresis, followed by electrophoretic transfer to pvdf membranes, and incubated in a solution containing trehalose (20 mg/ml), glucose oxidase (40 u/ml), phenazine methosulfate (0.06 mg/ml), and nitro blue ...19921621991
storage proteins of the larval root weevil diaprepes abbreviatus (coleoptera: curculionidae): riboflavin binding and subunit isolation.proteins present at high concentrations in hemolymph of the larval weevil diaprepes abbreviatus were previously shown to bind a synthetic coumarin, 7-amino-3-phenyl coumarin (coumarin-10). one of the two native proteins previously identified (protein i) is now shown to separate into two distinct bands (proteins ia and ib) using native gradient pore-limiting electrophoresis. the high concentration of proteins ia, ib, and ii in larval hemolymph, their disappearance from hemolymph upon pupation, an ...19921504322
brain factor from galleria mellonella (lepidoptera) stimulating silk gland activity.brain extracts from day 1-4 last instar larvae of galleria mellonella (lepidoptera) stimulate rna synthesis in cultured silk glands from day 3 last instar larvae. when the fibroin-synthesizing posterior parts of silk glands were incubated for 3 h in vitro in the presence of brain extract (0.1 brain equivalent), [3h]-uridine incorporation into rna was stimulated more than twofold. the stimulating effect of brain extract showed a dose response relationship. it is suggested that the heat-resistant ...19921381321
effect of galleria mellonella larvae preparation and honeybee products on cell cultures.1. the action of the extract from larvae of the great wax moth galleria mellonella l. used in russian folk medicine was studied. 2. two active components influencing the growth and morphological differentiation of cells in vitro were found. 3. the presence of these components in the extract was conditioned by consumption of honeybee products by galleria mellonella larvae. 4. cytotoxicity of honeybee products was studied.19921358524
the secretion of collagen by insects: uptake of [3h]proline by collagen-synthesizing cells in locusta migratoria and galleria mellonella.the uptake of [h3]proline by collagen-secreting cells of the locust, locusta migratoria, and wax-moth, galleria mellonella, has been investigated by electron autoradiography. the locust cells are around the ejaculatory duct and they secrete collagen in the young adult male, while the wax-moth cells are those which produce the dorsal mass of connective tissue on the abdominal nerve cord during the late pupal stage. the cells were exposed to [h3]proline either by injection of the [3h]proline into ...19761262412
influence of na+, k+, mg2+ and cooling on proteosynthesis in hemocytes of galleria mellonella. 19761248218
effect of heat-killed cells of achromobacter nematophilus poinar et thomas, and the fraction (endotoxin) isolated from them on galleria mellonella l. caterpillars. 19751192248
studies on the incorporation of carbon-14 in tissues of galleria mellonella. 19751184238
the multiplication of nodamura virus in insect and mammalian cell cultures.nodamura virus multiplied in mosquito cell lines, as determined by infectivivity assays in adult honey bees (apis mellifera) and wax moth larvae (galleria mellonella). titres of more than 10-7 and 10-5 bee ld50 /ml were obtained in culture fluids of aedes albopictus and aedes aegypti cells respectively after 10 days. comparable titres were obtained after several months, during which the cultures were subdivided up to six times. nodamura virus also multiplied in bhk cells and yielded titres of 10 ...19751168239
the basement membrane of the galleria mellonella fat body. 19751163145
concanavalin binding by the basal membrane of the ovarial tube in galleria mellonella (lepidoptera). 19751163129
glycoproteins in the basement membranes of the ovarial tube in galleria mellonella (lepidoptera). 19751163128
ultrastructure of the prothoracic gland cells of the last instar of galleria mellonella in relation to the state of development.the prothoracic glands of the last instar of galleria mellonella undergo characteristic alterations of their cellular fine structure closely related to cellular activity. during progressive secretory activity of the gland cells there are extensive plasmalemmal infoldings and formation of a pronounced lacunar system. mitochondria of the active cell phase are characterized by a specific increase in size and paler colour of the matrix. in contrast to the alterations, nuclei, er and golgi cisterns d ...19751149124
ultrastructural study of the prothoracic glands of galleria mellonella l. in the penultimate last larval, and pupal stages.the prothoracic gland (pgl) of galleria mellonella is a y-shaped, paired organ, consisting of 45-50 polyploid giant cells. the pgl cells are supplied by neurosecretory axons; release of neurosecretory granules (1000-1300 a in diameter) directly on the surface of pgl cells was frequently observed. based on ultrastructure, the last two larval instars can be divided into three phases: 1) restitutive phase immediately after moulting; 2) gradual activation in mid-intermoult as indicated by the logari ...19751131863
polynucleotide fragments from the 28s ribosomal rna of insects.if rna is extracted from the ribosomes which had been isolated from frozen-thawed tissue of galleria mellonella, the 28 s rna, when heated or treated with urea, dissociates into seven different species of polynucleotide fragments. they were designated as r1, r2, r3, r4, r5, r6, and r7, whose molecular weights were estimated to be 1.15x10-6, 0.75x10-6, 0.55x10-6, 0.40x10-6, 0.30x10-6, 0.25x10-6, 0.20x10-6 daltons, respectively. it is likely that r1 and r5 arise from a single nick in original 38 s ...19751129145
eye disk differentiation in the wax moth: induction in vitro.ecdysterone-induced differentiation of galleria mellonella compound eyes in vitro is critically dependent on the age of the donor. it is greatest in explants from young prepupae, decreases with increasing donor age, and is absent in explants from 4-6 hour pupae. failure of eye disks from pupae to differentiate in vitro seems to be the consequence of exposing them to culture conditions at a susceptible time in their development for, unlike eye disks from prepupae, they do not develop when removed ...19751127401
rna synthesis in imaginal disks of galleria mellonella: effects of alpha- and beta- ecdysone and fat body in vitro. 19751120913
control of corpora allata function in larvae of galleria mellonella. 19751115805
proteolytic cleavage of polyhedral protein during dissolution of inclusion bodies of the nuclear polyhedrosis viruses of bombyx mori and galleria mellonella under alkaline conditions. 19751089735
comparative chemical studies of the polyherdral proteins of the nuclear polyhedrosis viruses of bombyx mori and galleria mellonella. 19751089730
the ultrastructural organization of the visual system of the wax moth, galleria mellonella: the optic tract.the ultrastructural organization of the axons of retinula cells of the eye of the wax moth galleria mellonella are described. the axons traverse an appreciable distance between the basement membrane of the retina and the lamina ganglionaris of the optic lobe of the brain. the optic tract was reconstructed from serial thin sections. axons emanating from a single ommatidium are closely associated together in the optic tract. adjacent cartridges fuse together to form large clusters of axons (8 to 1 ...19761000590
the ultrastructural organization of the visual system of the wax moth, galleria mellonella: the retina.the ultrastructural organization of ommatidial components of the retina of the moth. galleria mellonella are described from electron microscopic observations. each ommatidium is composed of 12 common retinula cells and one basal eccentric cell. the retinula cells are connected together by a desmosomal strip along their length. the rhabdom occupies the basal thirty percent of the ommatidium and can be divided into nine segments of parallel microvilli. several cells may contribute to an individual ...19761000589
the granular cells of galleria mellonella during clotting and phagocytic reactions in vitro.light and electron-microscopic observations of the blood-cells (hemocytes) of the wax-moth galleria mellonella showed that hemolymph coagulation was initiated by the rapid release of material from the granular cells. during incubation for short terms in vitro these cells showed progressive degranulation as material derived from the granules was discharged into the hemolymph. attempts to determine the nature of this material by staining with ruthenium red proved mainly unsuccessful. when challeng ...1976982421
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